Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0006614

Physical Location & Seq

Genomic Coordinates Reverse (-)

20605349 .. 20615256

Gene Structure 9908 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2880 bp

ATGGAGCTCCAGAACACAGTGAAAGAAGCCCTCAATGCACTCTACCACCACCCCGACGACGGCGTCCGCCTGCAGGCCGATCGCTGGCTGCAGGACTTCCTGCGGACGCTGGACGCCTGGCAGGTTGCTGATAACTTGCTTCATGATGCAACTAGCAATCTAGAAACATTAATATTTTGTTCTCAGACCCTTAGAAGCAAGGTACAACGAGATATTGAAGAACTACCTTCTGAAGCTTTTCGCCCGTTACGTGATTCGTTAAATAACTTATTGAGAAAATTCCACAAAGGCCCCCCTAAAGTCAGAACTCAGATTAGCATTGCTGTGGCTGCTTTGGCTGTACATGTTCGCGCTGAAGATTGGGGGGATGGTGGGATTGTGAAGTGGCTTCGTGATGAGATGAATTTGCATCCAGAGTACATACCAGGACTTTTGGAGTTACTAACAGTTTTGCCTGAGGAAGTATTCAACTACAAAATTGCAGCTCGCCCGGAAAGACGCCGCCAATTTGAAAAGGAGCTTACTTCACAAATTGAGGTTGCTCTTGACATCCTGACTGCCTGTTTGAATATTACTGAACTTAAGGAGCAGGTTCTTGAAGCGTTTGCTTCTTGGCTTCGACTGAAACATGGGATCCCTGGATCTGTGCTTGCATCCCATCCATTGGTGCTCACAGCTCTCTCAAGCTTGAATTCTGAGCTACTTTCAGAGGCCGCAGTAAATGTTATATCTGAATTGATCCACTATACAGCAGCTGGAAGCTCAGGTGGTGTCTCTGTGCAGATGCCCTTAATTCAAGTTCTTGTCCCTCAAGTAATGAATCTGAAGGGACAGCTAAGAGATTCTTCAAAGGATGAGGAAGATGTGAAGGCCATTGCTCGCTTATTTTCTGACATGGGTGATTCATATGTTGAACTTATTGCAACTGGCTCAGATGAATCAATGTTGATTGTACATGCGTTATTGGAAGTTGCATCACACCCAGAGTACTATATTGCTTCCATGACATTCAACTTTTGGCACAGTCTTCAGGTGAACTTGACCAGAAGGGAATCGTATATTTCATTTGTGAATGAATCTTCCATTGAAGCTGAGAGAAATAGAAGGCTGCAAGTTTTTCGTCCAGCTTATGAGTCACTTGTATCCTTAGTTAGCTTTCGGATTCAATATCCTCAAGATTATCAAGACCTTTCAAATGAGGATCTTAAGGAGTTCAAGCATACTAGATATGCTGTTGCGGATGTCTTAATTGACGCAGCATCAGTTTTAGGGGGGGATGCCACACTTAGAATTCTTTACATGAAGCTTGATGAGGCTGCAGCATGCTGTCACAATGAACAAAGTGAATGGCGCCCAGCGGAAGCTGCTTTGTTCGGCATCCGGGCAATATCAAGTTATGTTTCAGCAGTTGAAGCTGAAGTAATGCCCAAGGTTATGGATAGGCTACTTAAATTGCCTCAACATCCTCAACTACTTCAGACAGTTTGCTTAACTATTGGAGCGTATTCAAAGTGGCTTGATTCTGCATCAGGCGGACTGTCCATACTGCCTTCAGTTCTAGATATCCTCATGAGTGGCATGGGTATATCTGAAGATTCTGCAGCAGCTGCAGCTGTGGCATTTAGACAGATTTGTGACGATTGCCGATTAAAGCTCTGTGGATGTTTGGATGGACTCTTCCATATATACCGTAGGGCAGTGAGTAGTGAAGGCAGTTTCAAGGTTGCTGCTGATGATTCGCTACATCTAGTTGAAGCCTTAAGCAAGGTCATTACTGAACTTCCTCCAGACCATGCTAAGAGGGCATTGGAGGCCTTGTGCTTGCCCGTTGTTACTCCTCTACAGGAAATTGTCAGCCAAGGCCCAGACACGCTAAAGAGCAAGCCTGCTCGTGATTTAACAGTTCACATCGATCGATTTGGATACATCTTTAGGTATGTGAATCATGCAGAAGCTGTGGCAGATGCAATTCAAAGACTTTGGCCAATTTTTAAAGCCATATTTGATCTTCGTGCTTGGGACATGCGGACAATGGAGTCTCTTTGCCGAGCTTGCAAATACGCTGTGAGGACTTCTGGAAGGTGCATGGGAATTACAATTGGAGCAATGCTAGAAGAGATTCAAGGTTTATATCAACAGCATCACCAACCGTGCTTCCTCTATTTATCTAGTGAAGTTATAAAGATATTCGGTTCTGACCCATCTTGTGCAAATTATTTGAAAAGTTTGATTGAAGCACTTTTTATGCACACAACACGTCTTCTCGCGAGCATTCAGGAATTTACTGCCAGACCTGATATAGCGGATGATTGTTTTCTGTTGGCATCAAGATGCATTCGCTATTGTCCTCAATTATTTATTCCATCAGCTGTATTTCCATCATTAGTAGATTGCTCGTTGATTGGGATTACAGTGCATCACAGAGAGGCATCAAATTCAATATTAACGTTCTTATCTGATATATTTGATCTTGGAAACTCTGCCGGGGGAGAACAATACTTACCAATCAGGAATTGTGTCATTCTTCCTCGAGGGTCCAGCATAACAAGAATTTTAATTGCTTCACTAACAGGAGCACTTCCAAGTTCCCGACTAGAATCGGTAACCTACACGCTACTATCTTTATCTCGAGCTTATGGGCCTCAGTCGTTGGAGTGGGCCAAGGAGAGTATTTCTTTAGTGCCTTTGACGGCTGTGACAGAAGTTGAGCGTTCGAGATTCTTGAAAGCATTGTCAGATGCTGCGTCCGGGGCTAACGTTAATGCCCTGTCTGTTCCAATTGAGGACCTATCGGATGTTTGCCGCCGTAATCGAACAGTTATGGAGATCGTTCAACAATCTTTGAGGCCACTTGAGTTGAACATAGCACCGGGATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0006606 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0010468 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0017038 GO:0019219 GO:0019222 GO:0031090 GO:0031323 GO:0031965 GO:0031967 GO:0031975 GO:0033036 GO:0033218 GO:0033365 GO:0034504 GO:0034613 GO:0042277 GO:0042802 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043484 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050789 GO:0050794 GO:0051169 GO:0051170 GO:0051171 GO:0051179 GO:0051234 GO:0051252 GO:0051641 GO:0051649 GO:0060255 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0080090

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

IBN_N Xpo1

Protein Analysis

960

Amino Acids

106.68

Weight (kDa)

5.53

Isoelectric Point (pI)

49.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Xpo1	PF08389	98 - 243	5.3e-19	Exportin 1-like protein
TPR_TNPO3_IPO13_2nd	PF24138	285 - 352	5.3e-15	Transportin-3/Importin-13 second TPR domain
TPR_TNPO3_IPO13_3rd	PF24140	473 - 562	5.8e-08	Transportin-3/Importin-13 third TPR domain
TPR_TNPO3_IPO13_4th	PF24139	655 - 754	3.7e-18	Transportin-3/Importin-13 fourth TPR domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006614)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G62600
fragaria_vesca	FvH4_5g16030 FvH4_5g16030 FvH4_5g16030 FvH4_5g16030 FvH4_5g16030 FvH4_5g16030
malus_domestica	MD06G1183500.v1.1 MD14G1189400.v1.1
prunus_persica	Prupe.5G182200_v2.0.a1 Prupe.6G220700_v2.0.a1
pyrus_communis	pycom06g16250 pycom14g15710
rosa_chinensis	RchiOBHm_Chr7g0180041
rosa_laevigata	RLG00000005300
rosa_multiflora	Rmu_co8039046.1_g000001 Rmu_ssc0000119.1_g000007 Rmu_ssc0000119.1_g000020 Rmu_ssc0000119.1_g000024
rosa_roxburghii	Rroxscaffold_3G00273180
rosa_rugosa	Rorug06G0430100
rosa_samantha	Rh7AG030300 Rh7BG030200 Rh7CG032000 Rh7DG031000
rosa_wichuraiana	Rw7G002520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2180
AasI	GACNNNNNNGTC	2 cut(s)	62, 2035
AbsI	CCTCGAGG	1 cut(s)	2529
Acc36I	ACCTGC	2 cut(s)	112, 580
AccB1I	GGYRCC	1 cut(s)	1350
AccB7I	CCANNNNNTGG	1 cut(s)	664
AccII	CGCG	2 cut(s)	351, 2267
AclI	AACGTT	2 cut(s)	2447, 2757
AclWI	GGATC	5 cut(s)	628, 641, 649, 733, 1209
AcoI	YGGCCR	1 cut(s)	1982
AcsI	RAATTY	7 cut(s)	278, 403, 691, 1290, 2279, 2434, 2550
AcuI	CTGAAG	8 cut(s)	252, 375, 845, 1013, 1437, 1460, 1536, 1611
AcyI	GRCGYC	4 cut(s)	63, 114, 499, 1351
AfaI	GTAC	5 cut(s)	204, 342, 419, 954, 989
AfiI	CCNNNNNNNGG	4 cut(s)	59, 73, 84, 664
AflII	CTTAAG	3 cut(s)	581, 1205, 1759
AflIII	ACRYGT	2 cut(s)	343, 2255
AjiI	CACGTC	1 cut(s)	2258
AjnI	CCWGG	3 cut(s)	116, 424, 637
Alw21I	GWGCWC	3 cut(s)	9, 672, 2578
Alw26I	GTCTC	2 cut(s)	778, 2043
AlwI	GGATC	5 cut(s)	628, 641, 649, 733, 1209
AlwNI	CAGNNNCTG	4 cut(s)	755, 1607, 1955, 2741
Ama87I	CYCGRG	2 cut(s)	2529, 2628
ApoI	RAATTY	7 cut(s)	278, 403, 691, 1290, 2279, 2434, 2550
ArsI	GACNNNNNNTTYG	2 cut(s)	547, 579
AseI	ATTAAT	1 cut(s)	170
Asp700I	GAANNNNTTC	2 cut(s)	237, 2118
AspLEI	GCGC	2 cut(s)	353, 1353
AspS9I	GGNCC	6 cut(s)	290, 1862, 2535, 2639, 2658, 2785
AsuC2I	CCSGG	5 cut(s)	491, 1382, 2485, 2749, 2871
AsuHPI	GGTGA	3 cut(s)	911, 1045, 2135
AvaI	CYCGRG	2 cut(s)	2529, 2628
AvaII	GGWCC	2 cut(s)	2535, 2785
AxyI	CCTNAGG	1 cut(s)	456
BalI	TGGCCA	1 cut(s)	1984
BamHI	GGATCC	1 cut(s)	633
BanI	GGYRCC	1 cut(s)	1350
BanII	GRGCYC	1 cut(s)	9
BauI	CACGAG	1 cut(s)	1890
BbsI	GAAGAC	2 cut(s)	1019, 2252
Bbv12I	GWGCWC	3 cut(s)	9, 672, 2578
BccI	CCATC	6 cut(s)	362, 666, 1664, 2209, 2371, 2386
BceAI	ACGGC	3 cut(s)	76, 2706, 2790
BcgI	CGANNNNNNTGC	4 cut(s)	62, 96, 1100, 1134
BciT130I	CCWGG	3 cut(s)	118, 426, 639
BciVI	GTATCC	2 cut(s)	1153, 1916
BcnI	CCSGG	5 cut(s)	491, 1382, 2485, 2749, 2871
BcoDI	GTCTC	2 cut(s)	778, 2043
BfaI	CTAG	8 cut(s)	153, 161, 1224, 1559, 1748, 2111, 2169, 2594
BfmI	CTRYAG	6 cut(s)	71, 89, 1317, 1599, 1608, 1841
BfoI	RGCGCY	1 cut(s)	1354
BfrI	CTTAAG	3 cut(s)	581, 1205, 1759
BfuAI	ACCTGC	2 cut(s)	112, 580
BfuI	GTATCC	2 cut(s)	1153, 1916
BmcAI	AGTACT	1 cut(s)	989
Bme1390I	CCNGG	8 cut(s)	118, 426, 491, 639, 1382, 2485, 2749, 2871
Bme18I	GGWCC	2 cut(s)	2535, 2785
BmeT110I	CYCGRG	2 cut(s)	2529, 2628
BmgBI	CACGTC	1 cut(s)	2258
BmgT120I	GGNCC	6 cut(s)	290, 1862, 2535, 2639, 2658, 2785
BmiI	GGNNCC	4 cut(s)	292, 635, 1352, 2536
BmrFI	CCNGG	8 cut(s)	118, 426, 491, 639, 1382, 2485, 2749, 2871
BpiI	GAAGAC	2 cut(s)	1019, 2252
BpmI	CTGGAG	1 cut(s)	1770
Bpu10I	CCTNAGC	1 cut(s)	763
BpuEI	CTTGAG	4 cut(s)	667, 795, 1158, 2873
BpuMI	CCSGG	5 cut(s)	491, 1382, 2485, 2749, 2871
Bsa29I	ATCGAT	2 cut(s)	1911, 1915
BsaAI	YACGTR	1 cut(s)	251
BsaHI	GRCGYC	4 cut(s)	63, 114, 499, 1351
BsaJI	CCNNGG	6 cut(s)	637, 1428, 1858, 2484, 2661, 2748
BsaXI	ACNNNNNCTCC	2 cut(s)	1802, 1832
Bsc4I	CCNNNNNNNGG	4 cut(s)	59, 73, 84, 664
Bse1I	ACTGG	1 cut(s)	931
Bse21I	CCTNAGG	1 cut(s)	456
Bse3DI	GCAATG	3 cut(s)	318, 873, 2112
BseBI	CCWGG	3 cut(s)	118, 426, 639
BseCI	ATCGAT	2 cut(s)	1911, 1915
BseDI	CCNNGG	6 cut(s)	637, 1428, 1858, 2484, 2661, 2748
BseLI	CCNNNNNNNGG	4 cut(s)	59, 73, 84, 664
BseMI	GCAATG	3 cut(s)	318, 873, 2112
BseMII	CTCAG	8 cut(s)	197, 323, 447, 687, 777, 945, 1083, 2657
BseNI	ACTGG	1 cut(s)	931
BseRI	GAGGAG	1 cut(s)	1827
BseYI	CCCAGC	1 cut(s)	1354
BsgI	GTGCAG	1 cut(s)	800
Bsh1236I	CGCG	2 cut(s)	351, 2267
Bsh1285I	CGRYCG	2 cut(s)	82, 1915
BshNI	GGYRCC	1 cut(s)	1350
BshVI	ATCGAT	2 cut(s)	1911, 1915
BsiEI	CGRYCG	2 cut(s)	82, 1915
BsiHKAI	GWGCWC	3 cut(s)	9, 672, 2578
BsiHKCI	CYCGRG	2 cut(s)	2529, 2628
BsiSI	CCGG	5 cut(s)	491, 1381, 2484, 2748, 2870
BslFI	GGGAC	3 cut(s)	791, 843, 2033
BslI	CCNNNNNNNGG	4 cut(s)	59, 73, 84, 664
BsmAI	GTCTC	2 cut(s)	778, 2043
BsmFI	GGGAC	3 cut(s)	791, 843, 2033
BsmI	GAATGC	2 cut(s)	2271, 2334
BsoBI	CYCGRG	2 cut(s)	2529, 2628
Bsp1286I	GDGCHC	3 cut(s)	9, 672, 2578
Bsp1407I	TGTACA	2 cut(s)	340, 952
Bsp68I	TCGCGA	1 cut(s)	2267
BspCNI	CTCAG	8 cut(s)	196, 322, 448, 688, 776, 944, 1084, 2656
BspDI	ATCGAT	2 cut(s)	1911, 1915
BspFNI	CGCG	2 cut(s)	351, 2267
BspHI	TCATGA	2 cut(s)	142, 1569
BspLI	GGNNCC	4 cut(s)	292, 635, 1352, 2536
BspMAI	CTGCAG	5 cut(s)	75, 93, 1321, 1603, 1612
BspMI	ACCTGC	2 cut(s)	112, 580
BspPI	GGATC	5 cut(s)	628, 641, 649, 733, 1209
BspT107I	GGYRCC	1 cut(s)	1350
BspTI	CTTAAG	3 cut(s)	581, 1205, 1759
BsrDI	GCAATG	3 cut(s)	318, 873, 2112
BsrGI	TGTACA	2 cut(s)	340, 952
BsrI	ACTGG	1 cut(s)	931
BssECI	CCNNGG	6 cut(s)	637, 1428, 1858, 2484, 2661, 2748
BssNI	GRCGYC	4 cut(s)	63, 114, 499, 1351
BssSI	CACGAG	1 cut(s)	1890
BssT1I	CCWWGG	3 cut(s)	1428, 1858, 2661
Bst2BI	CACGAG	1 cut(s)	1890
Bst2UI	CCWGG	3 cut(s)	118, 426, 639
Bst6I	CTCTTC	2 cut(s)	1682, 2109
BstACI	GRCGYC	4 cut(s)	63, 114, 499, 1351
BstAFI	CTTAAG	3 cut(s)	581, 1205, 1759
BstAPI	GCANNNNNTGC	1 cut(s)	1607
BstAUI	TGTACA	2 cut(s)	340, 952
BstBAI	YACGTR	1 cut(s)	251
BstEII	GGTNACC	1 cut(s)	2602
BstFNI	CGCG	2 cut(s)	351, 2267
BstH2I	RGCGCY	1 cut(s)	1354
BstHHI	GCGC	2 cut(s)	353, 1353
BstMAI	GTCTC	2 cut(s)	778, 2043
BstMCI	CGRYCG	2 cut(s)	82, 1915
BstNI	CCWGG	3 cut(s)	118, 426, 639
BstNSI	RCATGY	4 cut(s)	347, 959, 1326, 2026
BstPI	GGTNACC	1 cut(s)	2602
BstSCI	CCNGG	8 cut(s)	116, 424, 489, 637, 1380, 2483, 2747, 2869
BstSFI	CTRYAG	6 cut(s)	71, 89, 1317, 1599, 1608, 1841
BstUI	CGCG	2 cut(s)	351, 2267
BstV2I	GAAGAC	2 cut(s)	1019, 2252
BstX2I	RGATCY	3 cut(s)	633, 641, 1201
BstXI	CCANNNNNNTGG	1 cut(s)	1435
BstYI	RGATCY	3 cut(s)	633, 641, 1201
Bsu15I	ATCGAT	2 cut(s)	1911, 1915
Bsu36I	CCTNAGG	1 cut(s)	456
BsuI	GTATCC	2 cut(s)	1153, 1916
BsuTUI	ATCGAT	2 cut(s)	1911, 1915
BtrI	CACGTC	1 cut(s)	2258
BtsI	GCAGTG	1 cut(s)	1704
BtsIMutI	CAGTG	3 cut(s)	24, 1704, 2418
BtuMI	TCGCGA	1 cut(s)	2267
BveI	ACCTGC	2 cut(s)	112, 580
CaiI	CAGNNNCTG	4 cut(s)	755, 1607, 1955, 2741
CciI	TCATGA	2 cut(s)	142, 1569
CfoI	GCGC	2 cut(s)	353, 1353
Cfr13I	GGNCC	6 cut(s)	290, 1862, 2535, 2639, 2658, 2785
ClaI	ATCGAT	2 cut(s)	1911, 1915
CseI	GACGC	6 cut(s)	52, 115, 122, 507, 1262, 2733
Csp6I	GTAC	5 cut(s)	203, 341, 418, 953, 988
CspCI	CAANNNNNGTGG	2 cut(s)	2838, 2873
CviQI	GTAC	5 cut(s)	203, 341, 418, 953, 988
DinI	GGCGCC	1 cut(s)	1352
DraI	TTTAAA	1 cut(s)	1993
DrdI	GACNNNNNNGTC	2 cut(s)	62, 2035
DseDI	GACNNNNNNGTC	2 cut(s)	62, 2035
EaeI	YGGCCR	1 cut(s)	1982
Eam1104I	CTCTTC	2 cut(s)	1682, 2109
EarI	CTCTTC	2 cut(s)	1682, 2109
EciI	GGCGGA	2 cut(s)	56, 1548
Ecl136II	GAGCTC	1 cut(s)	7
Eco130I	CCWWGG	3 cut(s)	1428, 1858, 2661
Eco147I	AGGCCT	1 cut(s)	1814
Eco24I	GRGCYC	1 cut(s)	9
Eco32I	GATATC	1 cut(s)	1564
Eco47I	GGWCC	2 cut(s)	2535, 2785
Eco53kI	GAGCTC	1 cut(s)	7
Eco57I	CTGAAG	8 cut(s)	252, 375, 845, 1013, 1437, 1460, 1536, 1611
Eco81I	CCTNAGG	1 cut(s)	456
Eco88I	CYCGRG	2 cut(s)	2529, 2628
Eco91I	GGTNACC	1 cut(s)	2602
EcoICRI	GAGCTC	1 cut(s)	7
EcoO109I	RGGNCCY	2 cut(s)	290, 2785
EcoO65I	GGTNACC	1 cut(s)	2602
EcoRI	GAATTC	2 cut(s)	691, 1290
EcoRII	CCWGG	3 cut(s)	116, 424, 637
EcoRV	GATATC	1 cut(s)	1564
EcoT14I	CCWWGG	3 cut(s)	1428, 1858, 2661
EcoT22I	ATGCAT	1 cut(s)	2336
EcoT38I	GRGCYC	1 cut(s)	9
EgeI	GGCGCC	1 cut(s)	1352
EheI	GGCGCC	1 cut(s)	1352
ErhI	CCWWGG	3 cut(s)	1428, 1858, 2661
FalI	AAGNNNNNCTT	2 cut(s)	829, 861
FaqI	GGGAC	3 cut(s)	791, 843, 2033
FauNDI	CATATG	1 cut(s)	907
FriOI	GRGCYC	1 cut(s)	9
FspBI	CTAG	8 cut(s)	153, 161, 1224, 1559, 1748, 2111, 2169, 2594
GlaI	GCGC	2 cut(s)	352, 1352
GsaI	CCCAGC	1 cut(s)	1358
GsuI	CTGGAG	1 cut(s)	1770
HaeII	RGCGCY	1 cut(s)	1354
HapII	CCGG	5 cut(s)	491, 1381, 2484, 2748, 2870
HgaI	GACGC	6 cut(s)	52, 115, 122, 507, 1262, 2733
HhaI	GCGC	2 cut(s)	353, 1353
Hin1I	GRCGYC	4 cut(s)	63, 114, 499, 1351
Hin6I	GCGC	2 cut(s)	351, 1351
HinP1I	GCGC	2 cut(s)	351, 1351
HindIII	AAGCTT	3 cut(s)	234, 685, 1304
HpaII	CCGG	5 cut(s)	491, 1381, 2484, 2748, 2870
HphI	GGTGA	3 cut(s)	911, 1045, 2135
Hpy166II	GTNNAC	2 cut(s)	1036, 1906
Hpy8I	GTNNAC	2 cut(s)	1036, 1906
Hpy99I	CGWCG	2 cut(s)	59, 62
HpyAV	CCTTC	8 cut(s)	237, 820, 862, 1041, 1098, 1560, 1703, 2073
HpyCH4IV	ACGT	4 cut(s)	250, 2257, 2447, 2757
HpySE526I	ACGT	4 cut(s)	250, 2257, 2447, 2757
Hsp92I	GRCGYC	4 cut(s)	63, 114, 499, 1351
HspAI	GCGC	2 cut(s)	351, 1351
KasI	GGCGCC	1 cut(s)	1350
LmnI	GCTCC	7 cut(s)	4, 12, 517, 586, 1499, 2102, 2573
MaeI	CTAG	8 cut(s)	153, 161, 1224, 1559, 1748, 2111, 2169, 2594
MaeII	ACGT	4 cut(s)	250, 2257, 2447, 2757
MaeIII	GTNAC	8 cut(s)	246, 438, 1134, 1328, 1634, 1831, 2602, 2695
MfeI	CAATTG	2 cut(s)	2097, 2778
MflI	RGATCY	3 cut(s)	633, 641, 1201
MhlI	GDGCHC	3 cut(s)	9, 672, 2578
MlsI	TGGCCA	1 cut(s)	1984
MluNI	TGGCCA	1 cut(s)	1984
Mly113I	GGCGCC	1 cut(s)	1351
MlyI	GAGTC	3 cut(s)	1142, 1668, 2045
MmeI	TCCRAC	1 cut(s)	2631
Mox20I	TGGCCA	1 cut(s)	1984
Mph1103I	ATGCAT	1 cut(s)	2336
MroXI	GAANNNNTTC	2 cut(s)	237, 2118
MscI	TGGCCA	1 cut(s)	1984
MslI	CAYNNNNRTG	2 cut(s)	323, 2329
Msp20I	TGGCCA	1 cut(s)	1984
MspA1I	CMGCKG	5 cut(s)	755, 1358, 1607, 1613, 2369
MspCI	CTTAAG	3 cut(s)	581, 1205, 1759
MspI	CCGG	5 cut(s)	491, 1381, 2484, 2748, 2870
MspR9I	CCNGG	8 cut(s)	118, 426, 491, 639, 1382, 2485, 2749, 2871
MunI	CAATTG	2 cut(s)	2097, 2778
Mva1269I	GAATGC	2 cut(s)	2271, 2334
MvaI	CCWGG	3 cut(s)	118, 426, 639
MvnI	CGCG	2 cut(s)	351, 2267
NarI	GGCGCC	1 cut(s)	1351
NciI	CCSGG	5 cut(s)	491, 1382, 2485, 2749, 2871
NdeI	CATATG	1 cut(s)	907
NlaIV	GGNNCC	4 cut(s)	292, 635, 1352, 2536
NmeAIII	GCCGAG	1 cut(s)	2072
NmuCI	GTSAC	4 cut(s)	1134, 1328, 1634, 2695
NruI	TCGCGA	1 cut(s)	2267
NsiI	ATGCAT	1 cut(s)	2336
NspI	RCATGY	4 cut(s)	347, 959, 1326, 2026
PaeI	GCATGC	1 cut(s)	1326
PaeR7I	CTCGAG	2 cut(s)	2529, 2628
PagI	TCATGA	2 cut(s)	142, 1569
PceI	AGGCCT	1 cut(s)	1814
PciI	ACATGT	1 cut(s)	343
PcsI	WCGNNNNNNNCGW	1 cut(s)	247
PctI	GAATGC	2 cut(s)	2271, 2334
PdmI	GAANNNNTTC	2 cut(s)	237, 2118
PflFI	GACNNNGTC	1 cut(s)	62
PflMI	CCANNNNNTGG	1 cut(s)	664
Ple19I	CGATCG	2 cut(s)	82, 1915
PleI	GAGTC	3 cut(s)	1141, 1668, 2044
PluTI	GGCGCC	1 cut(s)	1354
PpsI	GAGTC	3 cut(s)	1141, 1668, 2044
Ppu21I	YACGTR	1 cut(s)	251
PpuMI	RGGWCCY	1 cut(s)	2785
PscI	ACATGT	1 cut(s)	343
PshBI	ATTAAT	1 cut(s)	170
PsiI	TTATAA	1 cut(s)	2180
Psp124BI	GAGCTC	1 cut(s)	9
Psp1406I	AACGTT	2 cut(s)	2447, 2757
Psp5II	RGGWCCY	1 cut(s)	2785
Psp6I	CCWGG	3 cut(s)	116, 424, 637
PspEI	GGTNACC	1 cut(s)	2602
PspFI	CCCAGC	1 cut(s)	1354
PspGI	CCWGG	3 cut(s)	116, 424, 637
PspN4I	GGNNCC	4 cut(s)	292, 635, 1352, 2536
PspPI	GGNCC	6 cut(s)	290, 1862, 2535, 2639, 2658, 2785
PspPPI	RGGWCCY	1 cut(s)	2785
PspXI	VCTCGAGB	1 cut(s)	2529
PsrI	GAACNNNNNNTAC	2 cut(s)	2484, 2516
PstI	CTGCAG	5 cut(s)	75, 93, 1321, 1603, 1612
PstNI	CAGNNNCTG	4 cut(s)	755, 1607, 1955, 2741
PsuI	RGATCY	3 cut(s)	633, 641, 1201
PsyI	GACNNNGTC	1 cut(s)	62
PvuI	CGATCG	2 cut(s)	82, 1915
PvuII	CAGCTG	4 cut(s)	755, 1607, 1613, 2369
RruI	TCGCGA	1 cut(s)	2267
RsaI	GTAC	5 cut(s)	204, 342, 419, 954, 989
RsaNI	GTAC	5 cut(s)	203, 341, 418, 953, 988
RseI	CAYNNNNRTG	2 cut(s)	323, 2329
SacI	GAGCTC	1 cut(s)	9
Sau96I	GGNCC	6 cut(s)	290, 1862, 2535, 2639, 2658, 2785
SbfI	CCTGCAGG	1 cut(s)	75
ScaI	AGTACT	1 cut(s)	989
SchI	GAGTC	3 cut(s)	1142, 1668, 2045
ScrFI	CCNGG	8 cut(s)	118, 426, 491, 639, 1382, 2485, 2749, 2871
SdaI	CCTGCAGG	1 cut(s)	75
SduI	GDGCHC	3 cut(s)	9, 672, 2578
SfcI	CTRYAG	6 cut(s)	71, 89, 1317, 1599, 1608, 1841
SfoI	GGCGCC	1 cut(s)	1352
Sfr274I	CTCGAG	2 cut(s)	2529, 2628
SinI	GGWCC	2 cut(s)	2535, 2785
SlaI	CTCGAG	2 cut(s)	2529, 2628
SmiMI	CAYNNNNRTG	2 cut(s)	323, 2329
SmlI	CTYRAG	9 cut(s)	581, 682, 810, 1173, 1205, 1759, 2529, 2628, 2852
SmoI	CTYRAG	9 cut(s)	581, 682, 810, 1173, 1205, 1759, 2529, 2628, 2852
SphI	GCATGC	1 cut(s)	1326
Sse8387I	CCTGCAGG	1 cut(s)	75
SseBI	AGGCCT	1 cut(s)	1814
SspDI	GGCGCC	1 cut(s)	1350
SspI	AATATT	3 cut(s)	174, 571, 2442
SspMI	CTAG	8 cut(s)	153, 161, 1224, 1559, 1748, 2111, 2169, 2594
SstI	GAGCTC	1 cut(s)	9
StuI	AGGCCT	1 cut(s)	1814
StyD4I	CCNGG	8 cut(s)	116, 424, 489, 637, 1380, 2483, 2747, 2869
StyI	CCWWGG	3 cut(s)	1428, 1858, 2661
TaiI	ACGT	4 cut(s)	253, 2260, 2450, 2760
TaqI	TCGA	7 cut(s)	619, 1911, 1915, 2530, 2629, 2714, 2812
TatI	WGTACW	4 cut(s)	340, 417, 952, 987
TauI	GCSGC	3 cut(s)	504, 716, 2805
TscAI	CASTG	3 cut(s)	24, 1704, 2418
TseFI	GTSAC	4 cut(s)	1134, 1328, 1634, 2695
Tsp45I	GTSAC	4 cut(s)	1134, 1328, 1634, 2695
TspDTI	ATGAA	9 cut(s)	131, 416, 833, 894, 951, 1053, 1089, 1316, 1350
TspRI	CASTG	3 cut(s)	24, 1704, 2418
Tth111I	GACNNNGTC	1 cut(s)	62
Van91I	CCANNNNNTGG	1 cut(s)	664
Vha464I	CTTAAG	3 cut(s)	581, 1205, 1759
VpaK11BI	GGWCC	2 cut(s)	2535, 2785
VspI	ATTAAT	1 cut(s)	170
XapI	RAATTY	7 cut(s)	278, 403, 691, 1290, 2279, 2434, 2550
XbaI	TCTAGA	2 cut(s)	160, 1558
XceI	RCATGY	4 cut(s)	347, 959, 1326, 2026
XhoI	CTCGAG	2 cut(s)	2529, 2628
XmnI	GAANNNNTTC	2 cut(s)	237, 2118
XspI	CTAG	8 cut(s)	153, 161, 1224, 1559, 1748, 2111, 2169, 2594
ZrmI	AGTACT	1 cut(s)	989
Zsp2I	ATGCAT	1 cut(s)	2336

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.