pycom06g18400

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004856
Physical Location & Seq
Forward (+)
22184130 .. 22185799
1670 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 957 bp
ATGTTTTATACCATGCCACCTATTAGTTGGTCTGATATGTCTTATTATCGTAAACAGATTCTTCCGCTGATACAAAAATACAAAGTTGTACATCTGAATAGAACTGATGCTCGACTTGCCAATAACGGACAACCTTTGGAGATTCAGAAACTTCGCTGCCGAGTTAATTTCAGTGCTCTGAGATTCACTTCTCAGATAGAGGAGTTGGGCAGAAGGGTTATCAGGCTTCTAAGGCAAAATGGTCCATTCCTTGTACTTCATCTTAGATATGAAATGGACATGTTGGCATTTTCTGGTTGTACTCAAGGTTGTAACATCGAGGAGGTGGAAGAGTTGACAAGAATGAGATATGCTTATCCCTGGTGGAAAGAGAAAATAATAAATTCTGACCTGAAAAGGAAAGATGGTTTCTGTCCTCTGACACCTGAAGAAACTGCTCTTACACTGAGTGCCCTTGACATTGACCGGGATATTCAGATTTATATCGCAGCTGGTGAAATCTATGGCGCAGAGAGGAGAATGGCAAGTCTTCGAGAGGCTTTTCCAAAATTGGTCAGAAAAGAGACACTGTTGGAACCATCAGACCTTAGGTTCTTTCAAAACCACTCATCCCAAATGGCAGCATTGGATTATCTTGTCTCATTGGAGAGTGATATCTTCGTTCCCACATATGATGGAAACATGGCTAAAGTGGTTGAAGGCCATCGCAGGTTTCTTGGGTTCAAGAAGACAATCTTACTGGACAGAAGGCTTCTTGTTGATTTGATAGACCGGTACACTAGTGGGTCATTGAATTGGGATGAATTTTCATCTGCAGTAAAGGGAGCTCATGCAAATCGCATGGGGAAACCAACTAATAGGTTGATGATCCAAGACCGACCTAAAGAGGAGGACTATTTCTATGCCAACCCAGTAGAGTGTTTGCAACCATCAGATGATGAACTAAGTAGTACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

319

Amino Acids

37.04

Weight (kDa)

7.62

Isoelectric Point (pI)

50.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 8 - 238 9.8e-69 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 699
AciI CCGC 1 cut(s) 65
AclWI GGATC 1 cut(s) 862
AcsI RAATTY 2 cut(s) 382, 803
AcuI CTGAAG 1 cut(s) 447
AdeI CACNNNGTG 1 cut(s) 449
AfaI GTAC 5 cut(s) 90, 255, 301, 776, 952
AflIII ACRYGT 1 cut(s) 279
AgeI ACCGGT 1 cut(s) 771
AgsI TTSAA 4 cut(s) 599, 698, 724, 793
AhlI ACTAGT 1 cut(s) 779
AjnI CCWGG 1 cut(s) 359
AluBI AGCT 2 cut(s) 491, 827
AluI AGCT 2 cut(s) 491, 827
Alw21I GWGCWC 2 cut(s) 178, 829
Alw26I GTCTC 2 cut(s) 557, 643
AlwI GGATC 1 cut(s) 862
AoxI GGCC 1 cut(s) 700
ApeKI GCWGC 3 cut(s) 156, 488, 620
ApoI RAATTY 2 cut(s) 382, 803
AsiGI ACCGGT 1 cut(s) 771
AspLEI GCGC 1 cut(s) 509
AspS9I GGNCC 1 cut(s) 242
AsuC2I CCSGG 1 cut(s) 467
AsuHPI GGTGA 1 cut(s) 506
AvaII GGWCC 1 cut(s) 242
AxyI CCTNAGG 1 cut(s) 587
BaeGI GKGCMC 1 cut(s) 454
BaeI ACNNNNGTAYC 2 cut(s) 766, 799
BanII GRGCYC 1 cut(s) 829
BbsI GAAGAC 2 cut(s) 521, 734
Bbv12I GWGCWC 2 cut(s) 178, 829
BbvI GCAGC 3 cut(s) 143, 500, 632
BccI CCATC 5 cut(s) 398, 586, 668, 711, 937
BciT130I CCWGG 1 cut(s) 361
BcnI CCSGG 1 cut(s) 467
BcoDI GTCTC 2 cut(s) 557, 643
BcuI ACTAGT 1 cut(s) 779
BfaI CTAG 1 cut(s) 780
BfmI CTRYAG 1 cut(s) 813
BfuAI ACCTGC 1 cut(s) 699
BisI GCNGC 3 cut(s) 157, 489, 621
BlsI GCNGC 3 cut(s) 158, 490, 622
Bme1390I CCNGG 2 cut(s) 361, 467
Bme18I GGWCC 1 cut(s) 242
BmgT120I GGNCC 1 cut(s) 242
BmiI GGNNCC 1 cut(s) 576
BmrFI CCNGG 2 cut(s) 361, 467
BmrI ACTGGG 1 cut(s) 905
BmsI GCATC 1 cut(s) 97
BmuI ACTGGG 1 cut(s) 905
BpiI GAAGAC 2 cut(s) 521, 734
BpuEI CTTGAG 1 cut(s) 288
BpuMI CCSGG 1 cut(s) 467
BsaAI YACGTR 1 cut(s) 954
BsaBI GATNNNNATC 1 cut(s) 482
BsaJI CCNNGG 1 cut(s) 359
BsaWI WCCGGW 1 cut(s) 771
Bse118I RCCGGY 1 cut(s) 771
Bse1I ACTGG 2 cut(s) 744, 911
Bse21I CCTNAGG 1 cut(s) 587
Bse8I GATNNNNATC 1 cut(s) 482
BseBI CCWGG 1 cut(s) 361
BseDI CCNNGG 1 cut(s) 359
BseGI GGATG 2 cut(s) 608, 805
BseJI GATNNNNATC 1 cut(s) 482
BseMII CTCAG 3 cut(s) 170, 206, 437
BseNI ACTGG 2 cut(s) 744, 911
BseRI GAGGAG 4 cut(s) 215, 335, 529, 902
BseSI GKGCMC 1 cut(s) 454
BseXI GCAGC 3 cut(s) 143, 500, 632
BshFI GGCC 1 cut(s) 702
BshTI ACCGGT 1 cut(s) 771
BsiHKAI GWGCWC 2 cut(s) 178, 829
BsiSI CCGG 2 cut(s) 466, 772
BsmAI GTCTC 2 cut(s) 557, 643
BsnI GGCC 1 cut(s) 702
Bsp1286I GDGCHC 3 cut(s) 178, 454, 829
Bsp1407I TGTACA 1 cut(s) 88
Bsp143I GATC 1 cut(s) 867
BspACI CCGC 1 cut(s) 65
BspANI GGCC 1 cut(s) 702
BspCNI CTCAG 3 cut(s) 171, 205, 438
BspLI GGNNCC 1 cut(s) 576
BspMAI CTGCAG 1 cut(s) 817
BspMI ACCTGC 1 cut(s) 699
BspPI GGATC 1 cut(s) 862
BsrFI RCCGGY 1 cut(s) 771
BsrGI TGTACA 1 cut(s) 88
BsrI ACTGG 2 cut(s) 744, 911
BssAI RCCGGY 1 cut(s) 771
BssECI CCNNGG 1 cut(s) 359
BssMI GATC 1 cut(s) 867
Bst2UI CCWGG 1 cut(s) 361
Bst4CI ACNGT 1 cut(s) 570
Bst6I CTCTTC 1 cut(s) 324
BstAUI TGTACA 1 cut(s) 88
BstBAI YACGTR 1 cut(s) 954
BstDEI CTNAG 7 cut(s) 179, 192, 230, 263, 446, 587, 944
BstF5I GGATG 2 cut(s) 608, 805
BstHHI GCGC 1 cut(s) 509
BstKTI GATC 1 cut(s) 870
BstMAI GTCTC 2 cut(s) 557, 643
BstMBI GATC 1 cut(s) 867
BstMWI GCNNNNNNNGC 2 cut(s) 116, 232
BstNI CCWGG 1 cut(s) 361
BstNSI RCATGY 1 cut(s) 283
BstSCI CCNGG 2 cut(s) 359, 465
BstSFI CTRYAG 1 cut(s) 813
BstSLI GKGCMC 1 cut(s) 454
BstV1I GCAGC 3 cut(s) 143, 500, 632
BstV2I GAAGAC 2 cut(s) 521, 734
Bsu36I CCTNAGG 1 cut(s) 587
BsuRI GGCC 1 cut(s) 702
BtgZI GCGATG 1 cut(s) 689
BtsCI GGATG 2 cut(s) 608, 805
BtsIMutI CAGTG 3 cut(s) 178, 443, 566
BveI ACCTGC 1 cut(s) 699
CfoI GCGC 1 cut(s) 509
Cfr10I RCCGGY 1 cut(s) 771
Cfr13I GGNCC 1 cut(s) 242
Csp6I GTAC 5 cut(s) 89, 254, 300, 775, 951
CspAI ACCGGT 1 cut(s) 771
CviAII CATG 5 cut(s) 13, 280, 682, 830, 841
CviJI RGCY 7 cut(s) 226, 491, 539, 686, 702, 751, 827
CviKI_1 RGCY 7 cut(s) 226, 491, 539, 686, 702, 751, 827
CviQI GTAC 5 cut(s) 89, 254, 300, 775, 951
DdeI CTNAG 7 cut(s) 179, 192, 230, 263, 446, 587, 944
DpnI GATC 1 cut(s) 869
DpnII GATC 1 cut(s) 867
DraIII CACNNNGTG 1 cut(s) 449
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
Ecl136II GAGCTC 1 cut(s) 827
Eco24I GRGCYC 1 cut(s) 829
Eco32I GATATC 1 cut(s) 655
Eco47I GGWCC 1 cut(s) 242
Eco53kI GAGCTC 1 cut(s) 827
Eco57I CTGAAG 1 cut(s) 447
Eco81I CCTNAGG 1 cut(s) 587
EcoICRI GAGCTC 1 cut(s) 827
EcoRII CCWGG 1 cut(s) 359
EcoRV GATATC 1 cut(s) 655
EcoT38I GRGCYC 1 cut(s) 829
FaeI CATG 5 cut(s) 16, 283, 685, 833, 844
FalI AAGNNNNNCTT 2 cut(s) 719, 751
FatI CATG 5 cut(s) 12, 279, 681, 829, 840
FauNDI CATATG 1 cut(s) 670
Fnu4HI GCNGC 3 cut(s) 157, 489, 621
FokI GGATG 2 cut(s) 595, 812
FriOI GRGCYC 1 cut(s) 829
Fsp4HI GCNGC 3 cut(s) 157, 489, 621
FspBI CTAG 1 cut(s) 780
GlaI GCGC 1 cut(s) 508
GluI GCNGC 3 cut(s) 157, 489, 621
HaeIII GGCC 1 cut(s) 702
HapII CCGG 2 cut(s) 466, 772
HhaI GCGC 1 cut(s) 509
Hin1II CATG 5 cut(s) 16, 283, 685, 833, 844
Hin6I GCGC 1 cut(s) 507
HinP1I GCGC 1 cut(s) 507
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HinfI GANTC 3 cut(s) 58, 142, 183
HpaII CCGG 2 cut(s) 466, 772
HphI GGTGA 1 cut(s) 506
Hpy166II GTNNAC 3 cut(s) 53, 336, 777
Hpy188III TCNNGA 2 cut(s) 533, 724
Hpy8I GTNNAC 3 cut(s) 53, 336, 777
HpyAV CCTTC 3 cut(s) 207, 692, 741
HpyCH4III ACNGT 1 cut(s) 570
HpyCH4IV ACGT 1 cut(s) 953
HpyCH4V TGCA 3 cut(s) 815, 833, 925
HpyF10VI GCNNNNNNNGC 2 cut(s) 116, 232
HpyF3I CTNAG 7 cut(s) 179, 192, 230, 263, 446, 587, 944
HpySE526I ACGT 1 cut(s) 953
Hsp92II CATG 5 cut(s) 16, 283, 685, 833, 844
HspAI GCGC 1 cut(s) 507
Kzo9I GATC 1 cut(s) 867
LmnI GCTCC 1 cut(s) 824
Lsp1109I GCAGC 3 cut(s) 143, 500, 632
LweI GCATC 1 cut(s) 97
MaeI CTAG 1 cut(s) 780
MaeII ACGT 1 cut(s) 953
MaeIII GTNAC 1 cut(s) 311
MalI GATC 1 cut(s) 869
MboI GATC 1 cut(s) 867
MboII GAAGA 6 cut(s) 53, 341, 440, 521, 649, 739
MhlI GDGCHC 3 cut(s) 178, 454, 829
MluCI AATT 5 cut(s) 166, 382, 548, 793, 803
MmeI TCCRAC 1 cut(s) 552
MnlI CCTC 8 cut(s) 193, 313, 316, 426, 507, 529, 880, 883
MseI TTAA 1 cut(s) 165
MspA1I CMGCKG 2 cut(s) 67, 491
MspI CCGG 2 cut(s) 466, 772
MspR9I CCNGG 2 cut(s) 361, 467
MvaI CCWGG 1 cut(s) 361
MwoI GCNNNNNNNGC 2 cut(s) 116, 232
NciI CCSGG 1 cut(s) 467
NdeI CATATG 1 cut(s) 670
NdeII GATC 1 cut(s) 867
NlaIII CATG 5 cut(s) 16, 283, 685, 833, 844
NlaIV GGNNCC 1 cut(s) 576
NmeAIII GCCGAG 1 cut(s) 185
NspI RCATGY 1 cut(s) 283
PciI ACATGT 1 cut(s) 279
PfeI GAWTC 3 cut(s) 58, 142, 183
PinAI ACCGGT 1 cut(s) 771
PkrI GCNGC 3 cut(s) 158, 490, 622
Ppu21I YACGTR 1 cut(s) 954
PscI ACATGT 1 cut(s) 279
Psp124BI GAGCTC 1 cut(s) 829
Psp6I CCWGG 1 cut(s) 359
PspGI CCWGG 1 cut(s) 359
PspN4I GGNNCC 1 cut(s) 576
PspPI GGNCC 1 cut(s) 242
PstI CTGCAG 1 cut(s) 817
PvuII CAGCTG 1 cut(s) 491
RsaI GTAC 5 cut(s) 90, 255, 301, 776, 952
RsaNI GTAC 5 cut(s) 89, 254, 300, 775, 951
SacI GAGCTC 1 cut(s) 829
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 3 cut(s) 157, 489, 621
Sau3AI GATC 1 cut(s) 867
Sau96I GGNCC 1 cut(s) 242
ScrFI CCNGG 2 cut(s) 361, 467
SduI GDGCHC 3 cut(s) 178, 454, 829
SfaNI GCATC 1 cut(s) 97
SfcI CTRYAG 1 cut(s) 813
SinI GGWCC 1 cut(s) 242
SmlI CTYRAG 1 cut(s) 303
SmoI CTYRAG 1 cut(s) 303
SpeI ACTAGT 1 cut(s) 779
Sse9I AATT 5 cut(s) 166, 382, 548, 793, 803
SsiI CCGC 1 cut(s) 65
SspMI CTAG 1 cut(s) 780
SstI GAGCTC 1 cut(s) 829
StyD4I CCNGG 2 cut(s) 359, 465
TaaI ACNGT 1 cut(s) 570
TaiI ACGT 1 cut(s) 956
TaqI TCGA 3 cut(s) 112, 318, 532
TaqII GACCGA 1 cut(s) 891
TasI AATT 5 cut(s) 166, 382, 548, 793, 803
TatI WGTACW 3 cut(s) 88, 253, 299
TfiI GAWTC 3 cut(s) 58, 142, 183
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TscAI CASTG 3 cut(s) 178, 450, 573
TseI GCWGC 3 cut(s) 156, 488, 620
TspDTI ATGAA 5 cut(s) 248, 285, 798, 816, 954
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 3 cut(s) 178, 450, 573
VpaK11BI GGWCC 1 cut(s) 242
XapI RAATTY 2 cut(s) 382, 803
XceI RCATGY 1 cut(s) 283
XcmI CCANNNNNNNNNTGG 1 cut(s) 24
XspI CTAG 1 cut(s) 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.