pycom06g19660

RING-H2 zinc finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0007225
Physical Location & Seq
Forward (+)
23004292 .. 23006694
2403 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1128 bp
ATGCCTCTCACAGAGAGCTCAACTGCAGGAGCAGAGCACATGAAATGGAGAAGACCCAGAACCCAATTCAACCAACCCATTTCAGAAACGGATCCAGCCACCTCCCCCCTCCAGTCCACCCGCTGCAAATCTACCATCTCCTCCCTCCTCCTCTCCAGCTTCACCACCTCCACCAACAACGAAGCCTTACCCACAAGCCTTACCATCAAGAAAAAGACAAACTTTCCCTCGTCGTCGTTCAGAGGCATGGGCTGCGCCGCCTCCGCCTCGCAGCAAGTCTCGGTGCCGGAGGAGATTCGGAACTCCGCCGATTGGAAGGGAAAGAAGGTGAAGAAGAAAAAGGAGAAGAAGAGCGCCGCCGCCAAGGGCAGCGCAAGCAATGAGAAGAATGAATACCGGGATGTCGGTGATGGCGGGCCCACCATTGGTATAAATTCTGCAACTTGTATGGATTTTCAGGATGTTTGGTGCGGCCCCGGAATTGGGTTCTCGCCGGAAACTGCCGGCTCGGTGGATTGCGTGGTGGCGAGGAGGAATGTCTCCGGCAGAGGCAAGATTTATGGGGATAATAATAAGATGAGCAGCCACAGGGAGCGTCCTTGTTTCGCAAGGCGAAACGTGAGCCCTGAAACCATCTCGTTTCTGGATTCTGAACCGGATTTTGTCTCGAGCCACCCTGAATCAGAGGTATTTGGAAGTAGGTGTTTTCGGCATGTCCGGCATCCATCCCCTGAAGGCCTTGCAGAGATTATGCTGTTTCAAAGTAGTCTTATGATGGGTGGAAGAATGGATAGATTCCGTGACTGGAGACTCGATGTTGATAGCATGTCATATGAGCAACTGCTCGAGTTAGGCGAGAGAATGGGCCACGTGAGTACTGGATTAAAAGAAGATGAAATAAACCACTGCCTTCGAAAGATTAAGCTTCCAATGTCGAGTGACGCGGCATCACATTCATCTGGGAAAGTAGATAGCAAATGCATCATTTGTCAGGAGGAGTATGAAGCAGATGACGAGATGGGCAAACTGCACTGCGGACATAACTTCCACTTACAATGTATAAAACAGTGGCTCACACAAAAGAATACGTGCCCTTTCTGCAAGGTCGAAGCAATATCTAGATGCTAG

Protein Analysis

376

Amino Acids

41.6

Weight (kDa)

8.44

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 322 - 368 3e-11 RING-H2 zinc finger domain
zf-RING_2 PF13639 326 - 368 4.1e-14 Ring finger domain
zf-C3HC4_2 PF13923 326 - 367 2.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 327 - 365 1.3e-07 RING-type zinc-finger
zf-C3HC4 PF00097 327 - 367 5.5e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 283
AccII CGCG 1 cut(s) 944
AclWI GGATC 2 cut(s) 86, 99
AcsI RAATTY 1 cut(s) 433
AcuI CTGAAG 1 cut(s) 753
AcvI CACGTG 1 cut(s) 871
AfaI GTAC 1 cut(s) 877
AfiI CCNNNNNNNGG 4 cut(s) 312, 425, 482, 483
AgsI TTSAA 2 cut(s) 70, 761
AluBI AGCT 3 cut(s) 18, 159, 925
AluI AGCT 3 cut(s) 18, 159, 925
Alw21I GWGCWC 2 cut(s) 20, 39
Alw26I GTCTC 4 cut(s) 283, 544, 670, 802
AlwI GGATC 2 cut(s) 86, 99
Ama87I CYCGRG 2 cut(s) 667, 845
AoxI GGCC 4 cut(s) 416, 472, 736, 865
ApaI GGGCCC 1 cut(s) 420
ApeKI GCWGC 5 cut(s) 123, 252, 271, 369, 582
ApoI RAATTY 1 cut(s) 433
AspLEI GCGC 3 cut(s) 257, 356, 374
AspS9I GGNCC 4 cut(s) 416, 417, 473, 865
AsuC2I CCSGG 2 cut(s) 398, 477
AsuHPI GGTGA 3 cut(s) 154, 340, 419
AsuII TTCGAA 1 cut(s) 913
AvaI CYCGRG 2 cut(s) 667, 845
BaeGI GKGCMC 2 cut(s) 420, 1094
BamHI GGATCC 1 cut(s) 91
BanI GGYRCC 1 cut(s) 283
BanII GRGCYC 3 cut(s) 20, 420, 626
BarI GAAGNNNNNNTAC 2 cut(s) 377, 409
BbrPI CACGTG 1 cut(s) 871
BbsI GAAGAC 1 cut(s) 58
Bbv12I GWGCWC 2 cut(s) 20, 39
BbvI GCAGC 5 cut(s) 110, 239, 283, 381, 594
BccI CCATC 7 cut(s) 143, 212, 404, 641, 733, 769, 1012
BcnI CCSGG 2 cut(s) 398, 477
BcoDI GTCTC 4 cut(s) 283, 544, 670, 802
BfaI CTAG 2 cut(s) 1119, 1126
BfmI CTRYAG 1 cut(s) 24
BfoI RGCGCY 1 cut(s) 357
BmcAI AGTACT 1 cut(s) 877
Bme1390I CCNGG 2 cut(s) 398, 477
BmeT110I CYCGRG 2 cut(s) 667, 845
BmgT120I GGNCC 4 cut(s) 416, 417, 473, 865
BmiI GGNNCC 4 cut(s) 93, 285, 418, 475
BmrFI CCNGG 2 cut(s) 398, 477
BmsI GCATC 4 cut(s) 730, 956, 990, 1112
BpiI GAAGAC 1 cut(s) 58
BpmI CTGGAG 3 cut(s) 95, 139, 826
Bpu14I TTCGAA 1 cut(s) 913
BpuMI CCSGG 2 cut(s) 398, 477
BsaAI YACGTR 2 cut(s) 871, 1089
BsaJI CCNNGG 2 cut(s) 363, 475
BsaWI WCCGGW 1 cut(s) 655
Bsc4I CCNNNNNNNGG 4 cut(s) 312, 425, 482, 483
Bse118I RCCGGY 1 cut(s) 503
Bse1I ACTGG 3 cut(s) 112, 809, 883
Bse3DI GCAATG 1 cut(s) 385
BseDI CCNNGG 2 cut(s) 363, 475
BseGI GGATG 4 cut(s) 406, 466, 721, 725
BseLI CCNNNNNNNGG 4 cut(s) 312, 425, 482, 483
BseMI GCAATG 1 cut(s) 385
BseNI ACTGG 3 cut(s) 112, 809, 883
BseRI GAGGAG 6 cut(s) 130, 137, 140, 305, 544, 1010
BseSI GKGCMC 2 cut(s) 420, 1094
BseXI GCAGC 5 cut(s) 110, 239, 283, 381, 594
BsgI GTGCAG 1 cut(s) 1013
Bsh1236I CGCG 1 cut(s) 944
BshFI GGCC 4 cut(s) 418, 474, 738, 867
BshNI GGYRCC 1 cut(s) 283
BsiHKAI GWGCWC 2 cut(s) 20, 39
BsiHKCI CYCGRG 2 cut(s) 667, 845
BsiSI CCGG 8 cut(s) 287, 397, 477, 494, 504, 543, 656, 718
BslI CCNNNNNNNGG 4 cut(s) 312, 425, 482, 483
BsmAI GTCTC 4 cut(s) 283, 544, 670, 802
BsnI GGCC 4 cut(s) 418, 474, 738, 867
BsoBI CYCGRG 2 cut(s) 667, 845
Bsp119I TTCGAA 1 cut(s) 913
Bsp120I GGGCCC 1 cut(s) 416
Bsp1286I GDGCHC 5 cut(s) 20, 39, 420, 626, 1094
Bsp143I GATC 1 cut(s) 91
BspANI GGCC 4 cut(s) 418, 474, 738, 867
BspFNI CGCG 1 cut(s) 944
BspLI GGNNCC 4 cut(s) 93, 285, 418, 475
BspMAI CTGCAG 1 cut(s) 28
BspPI GGATC 2 cut(s) 86, 99
BspQI GCTCTTC 1 cut(s) 344
BspT104I TTCGAA 1 cut(s) 913
BspT107I GGYRCC 1 cut(s) 283
BsrDI GCAATG 1 cut(s) 385
BsrFI RCCGGY 1 cut(s) 503
BsrI ACTGG 3 cut(s) 112, 809, 883
BssAI RCCGGY 1 cut(s) 503
BssECI CCNNGG 2 cut(s) 363, 475
BssMI GATC 1 cut(s) 91
BssT1I CCWWGG 1 cut(s) 363
Bst4CI ACNGT 1 cut(s) 1068
Bst6I CTCTTC 1 cut(s) 344
BstAPI GCANNNNNTGC 1 cut(s) 252
BstBAI YACGTR 2 cut(s) 871, 1089
BstBI TTCGAA 1 cut(s) 913
BstC8I GCNNGC 3 cut(s) 376, 416, 505
BstF5I GGATG 4 cut(s) 406, 466, 721, 725
BstFNI CGCG 1 cut(s) 944
BstH2I RGCGCY 1 cut(s) 357
BstHHI GCGC 3 cut(s) 257, 356, 374
BstKTI GATC 1 cut(s) 94
BstMAI GTCTC 4 cut(s) 283, 544, 670, 802
BstMBI GATC 1 cut(s) 91
BstMWI GCNNNNNNNGC 5 cut(s) 252, 263, 375, 718, 1098
BstNSI RCATGY 2 cut(s) 716, 829
BstSCI CCNGG 2 cut(s) 396, 475
BstSFI CTRYAG 1 cut(s) 24
BstSLI GKGCMC 2 cut(s) 420, 1094
BstUI CGCG 1 cut(s) 944
BstV1I GCAGC 5 cut(s) 110, 239, 283, 381, 594
BstV2I GAAGAC 1 cut(s) 58
BstX2I RGATCY 1 cut(s) 91
BstYI RGATCY 1 cut(s) 91
BsuRI GGCC 4 cut(s) 418, 474, 738, 867
BtsCI GGATG 4 cut(s) 406, 466, 721, 725
BtsI GCAGTG 2 cut(s) 904, 1030
BtsIMutI CAGTG 3 cut(s) 904, 1030, 1073
Cac8I GCNNGC 3 cut(s) 376, 416, 505
CfoI GCGC 3 cut(s) 257, 356, 374
Cfr10I RCCGGY 1 cut(s) 503
Cfr13I GGNCC 4 cut(s) 416, 417, 473, 865
CseI GACGC 2 cut(s) 584, 950
Csp6I GTAC 1 cut(s) 876
CviAII CATG 4 cut(s) 40, 247, 713, 826
CviQI GTAC 1 cut(s) 876
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
Eam1104I CTCTTC 1 cut(s) 344
EarI CTCTTC 1 cut(s) 344
EciI GGCGGA 2 cut(s) 253, 295
Ecl136II GAGCTC 1 cut(s) 18
Eco130I CCWWGG 1 cut(s) 363
Eco147I AGGCCT 1 cut(s) 738
Eco24I GRGCYC 3 cut(s) 20, 420, 626
Eco53kI GAGCTC 1 cut(s) 18
Eco57I CTGAAG 1 cut(s) 753
Eco72I CACGTG 1 cut(s) 871
Eco88I CYCGRG 2 cut(s) 667, 845
EcoICRI GAGCTC 1 cut(s) 18
EcoT14I CCWWGG 1 cut(s) 363
EcoT22I ATGCAT 1 cut(s) 983
EcoT38I GRGCYC 3 cut(s) 20, 420, 626
ErhI CCWWGG 1 cut(s) 363
FaeI CATG 4 cut(s) 43, 250, 716, 829
FalI AAGNNNNNCTT 2 cut(s) 206, 238
FatI CATG 4 cut(s) 39, 246, 712, 825
FauI CCCGC 2 cut(s) 128, 407
FauNDI CATATG 1 cut(s) 832
FokI GGATG 4 cut(s) 413, 473, 708, 712
FriOI GRGCYC 3 cut(s) 20, 420, 626
FspBI CTAG 2 cut(s) 1119, 1126
GlaI GCGC 3 cut(s) 256, 355, 373
GsuI CTGGAG 3 cut(s) 95, 139, 826
HaeII RGCGCY 1 cut(s) 357
HaeIII GGCC 4 cut(s) 418, 474, 738, 867
HapII CCGG 8 cut(s) 287, 397, 477, 494, 504, 543, 656, 718
HgaI GACGC 2 cut(s) 584, 950
HhaI GCGC 3 cut(s) 257, 356, 374
Hin1II CATG 4 cut(s) 43, 250, 716, 829
Hin6I GCGC 3 cut(s) 255, 354, 372
HinP1I GCGC 3 cut(s) 255, 354, 372
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 5 cut(s) 295, 647, 680, 795, 810
HpaII CCGG 8 cut(s) 287, 397, 477, 494, 504, 543, 656, 718
HphI GGTGA 3 cut(s) 154, 340, 419
Hpy166II GTNNAC 1 cut(s) 117
Hpy188I TCNGA 5 cut(s) 85, 242, 300, 652, 685
Hpy188III TCNNGA 6 cut(s) 208, 458, 644, 667, 992, 1119
Hpy8I GTNNAC 1 cut(s) 117
Hpy99I CGWCG 2 cut(s) 235, 238
HpyAV CCTTC 4 cut(s) 310, 319, 728, 920
HpyCH4III ACNGT 1 cut(s) 1068
HpyCH4IV ACGT 3 cut(s) 618, 870, 1088
HpyCH4V TGCA 7 cut(s) 26, 126, 440, 743, 981, 1030, 1101
HpyF10VI GCNNNNNNNGC 5 cut(s) 252, 263, 375, 718, 1098
HpySE526I ACGT 3 cut(s) 618, 870, 1088
Hsp92II CATG 4 cut(s) 43, 250, 716, 829
HspAI GCGC 3 cut(s) 255, 354, 372
KroI GCCGGC 1 cut(s) 503
KroNI GCCGGC 1 cut(s) 505
Kzo9I GATC 1 cut(s) 91
LguI GCTCTTC 1 cut(s) 344
LmnI GCTCC 2 cut(s) 29, 592
Lsp1109I GCAGC 5 cut(s) 110, 239, 283, 381, 594
LweI GCATC 4 cut(s) 730, 956, 990, 1112
MaeI CTAG 2 cut(s) 1119, 1126
MaeII ACGT 3 cut(s) 618, 870, 1088
MaeIII GTNAC 2 cut(s) 800, 938
MalI GATC 1 cut(s) 93
MboI GATC 1 cut(s) 91
MboII GAAGA 8 cut(s) 63, 343, 346, 358, 361, 397, 795, 902
MflI RGATCY 1 cut(s) 91
MhlI GDGCHC 5 cut(s) 20, 39, 420, 626, 1094
MluCI AATT 3 cut(s) 65, 433, 480
MlyI GAGTC 1 cut(s) 804
Mph1103I ATGCAT 1 cut(s) 983
MroNI GCCGGC 1 cut(s) 503
MseI TTAA 2 cut(s) 884, 921
MspA1I CMGCKG 1 cut(s) 123
MspI CCGG 8 cut(s) 287, 397, 477, 494, 504, 543, 656, 718
MspR9I CCNGG 2 cut(s) 398, 477
MvnI CGCG 1 cut(s) 944
MwoI GCNNNNNNNGC 5 cut(s) 252, 263, 375, 718, 1098
NaeI GCCGGC 1 cut(s) 505
NciI CCSGG 2 cut(s) 398, 477
NdeI CATATG 1 cut(s) 832
NdeII GATC 1 cut(s) 91
NgoMIV GCCGGC 1 cut(s) 503
NlaIII CATG 4 cut(s) 43, 250, 716, 829
NlaIV GGNNCC 4 cut(s) 93, 285, 418, 475
NmuCI GTSAC 2 cut(s) 800, 938
NsiI ATGCAT 1 cut(s) 983
NspI RCATGY 2 cut(s) 716, 829
NspV TTCGAA 1 cut(s) 913
PaeR7I CTCGAG 2 cut(s) 667, 845
PceI AGGCCT 1 cut(s) 738
PciSI GCTCTTC 1 cut(s) 344
PcsI WCGNNNNNNNCGW 1 cut(s) 852
PdiI GCCGGC 1 cut(s) 505
PfeI GAWTC 4 cut(s) 295, 647, 680, 795
PleI GAGTC 1 cut(s) 804
PmaCI CACGTG 1 cut(s) 871
PmlI CACGTG 1 cut(s) 871
PpsI GAGTC 1 cut(s) 804
Ppu21I YACGTR 2 cut(s) 871, 1089
Psp124BI GAGCTC 1 cut(s) 20
PspCI CACGTG 1 cut(s) 871
PspN4I GGNNCC 4 cut(s) 93, 285, 418, 475
PspOMI GGGCCC 1 cut(s) 416
PspPI GGNCC 4 cut(s) 416, 417, 473, 865
PspXI VCTCGAGB 1 cut(s) 845
PstI CTGCAG 1 cut(s) 28
PsuI RGATCY 1 cut(s) 91
RsaI GTAC 1 cut(s) 877
RsaNI GTAC 1 cut(s) 876
SacI GAGCTC 1 cut(s) 20
SapI GCTCTTC 1 cut(s) 344
SaqAI TTAA 2 cut(s) 884, 921
Sau3AI GATC 1 cut(s) 91
Sau96I GGNCC 4 cut(s) 416, 417, 473, 865
ScaI AGTACT 1 cut(s) 877
SchI GAGTC 1 cut(s) 804
ScrFI CCNGG 2 cut(s) 398, 477
SduI GDGCHC 5 cut(s) 20, 39, 420, 626, 1094
SfaNI GCATC 4 cut(s) 730, 956, 990, 1112
SfcI CTRYAG 1 cut(s) 24
Sfr274I CTCGAG 2 cut(s) 667, 845
SfuI TTCGAA 1 cut(s) 913
SlaI CTCGAG 2 cut(s) 667, 845
SmlI CTYRAG 2 cut(s) 667, 845
SmoI CTYRAG 2 cut(s) 667, 845
Sse9I AATT 3 cut(s) 65, 433, 480
SseBI AGGCCT 1 cut(s) 738
SspMI CTAG 2 cut(s) 1119, 1126
SstI GAGCTC 1 cut(s) 20
StuI AGGCCT 1 cut(s) 738
StyD4I CCNGG 2 cut(s) 396, 475
StyI CCWWGG 1 cut(s) 363
TaaI ACNGT 1 cut(s) 1068
TaiI ACGT 3 cut(s) 621, 873, 1091
TaqI TCGA 6 cut(s) 668, 813, 846, 913, 935, 1107
TasI AATT 3 cut(s) 65, 433, 480
TatI WGTACW 1 cut(s) 875
TauI GCSGC 5 cut(s) 260, 359, 362, 474, 947
TfiI GAWTC 4 cut(s) 295, 647, 680, 795
Tru1I TTAA 2 cut(s) 884, 921
Tru9I TTAA 2 cut(s) 884, 921
TscAI CASTG 3 cut(s) 911, 1037, 1073
TseFI GTSAC 2 cut(s) 800, 938
TseI GCWGC 5 cut(s) 123, 252, 271, 369, 582
Tsp45I GTSAC 2 cut(s) 800, 938
TspDTI ATGAA 5 cut(s) 56, 405, 909, 945, 1017
TspGWI ACGGA 2 cut(s) 104, 788
TspRI CASTG 3 cut(s) 911, 1037, 1073
XapI RAATTY 1 cut(s) 433
XbaI TCTAGA 1 cut(s) 1118
XceI RCATGY 2 cut(s) 716, 829
XcmI CCANNNNNNNNNTGG 2 cut(s) 640, 875
XhoI CTCGAG 2 cut(s) 667, 845
XspI CTAG 2 cut(s) 1119, 1126
ZrmI AGTACT 1 cut(s) 877
Zsp2I ATGCAT 1 cut(s) 983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.