pycom06g19780

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0005794
Physical Location & Seq
Forward (+)
23091781 .. 23094425
2645 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1035 bp
ATGACTGAAACCGAAACCGCCAATCCAGTCCCGACGAACGAGAGTGCAAGAGAGGAGGCGGAGGCCGCGTCAAAGCAAAAGAACAAGCGGGAAGGCGATTCCAGCAATGCTATTTCAGCTGCCAAGAAGTCAAAAACGGAAGAAGATGAAGACCCAGAAGGTGATATGAGCAAAAGGGAAATGGGTTTTGAAATCGAGGCCGATGCGGCGGAGCATAAGGGGCTGAGGCACGCCATGGAGGACGGTTGGGTTGTGCTCTTGGAAGCCGCCGGCGAGGATTTTCCCGGAAATTTGAGATGTGCGCATTTTGCAGTTTATGATGGGCATGGAGGTCGATTGGCTGCGGAGTATGCTCAGAAGCATCTGAACACGAATGTGCTTTCGGCCGGATTACCACGTGAGTTGTTGGATATAAAAGCAGCCAAGAAAGCCATACTTGACGGTTTTCGGAAAACTGATGAGTCTCTTCTTCGAGAAAGTGCTGCAGGGGGGTGGCAGGATGGTGCTACAGCTGTATGTGTTTGGATATTAGGAAAAACTGTTTTTGTTGCTAACATTGGGGATGCAAAAGCAGTGGTAGCAAGATCATCTCCTGCTGAGGGGTTGCAAAATGGTTCTAATGAAGCGTGTCCCCTGAAGGCGATTGTTTTGACAAGGGAACACAAAGCCATCTATGCACAGGAGCGTGCTCGTATTCAGAAGGCTGGTGGCAATGTGAGTTCAAATGGACGACTACAGGGGCGCCTTGAAGTTTCTAGGGCCTTTGGAGATCGCCCGTTCAAAAAGGTGGGCGTTATTGCAACCCCAGATGTTCATTCTTTTGACCTTACAGAAAGAGAGCACTTCATAATTCTTGGTTGCGACGGCCTCTGGGGGGTAATTGGACCAAGTGATGCCGTTGATTTTGTGCAGAAACTGTTGAAGGAGGGACTACCTGTTGCAACTATAAGCCGTCGTCTTGTAAAAGAAGCTGTGCGAGAGAGGCGCTGTAAAGATAACTGCACTGCGATCATAATCGCGTTCAGGCACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

345

Amino Acids

37.34

Weight (kDa)

6.35

Isoelectric Point (pI)

48.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 69 - 332 1.1e-53 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 303
AccB1I GGYRCC 1 cut(s) 741
AccII CGCG 2 cut(s) 68, 1019
AciI CCGC 8 cut(s) 18, 59, 66, 88, 206, 209, 267, 344
AcoI YGGCCR 1 cut(s) 384
AcsI RAATTY 1 cut(s) 289
AcuI CTGAAG 1 cut(s) 656
AcvI CACGTG 1 cut(s) 398
AcyI GRCGYC 1 cut(s) 742
AfiI CCNNNNNNNGG 2 cut(s) 599, 874
AgsI TTSAA 5 cut(s) 191, 723, 749, 781, 922
AleI CACNNNNGTG 1 cut(s) 374
AluBI AGCT 3 cut(s) 119, 512, 971
AluI AGCT 3 cut(s) 119, 512, 971
Alw21I GWGCWC 3 cut(s) 258, 691, 843
Alw26I GTCTC 1 cut(s) 468
AlwNI CAGNNNCTG 1 cut(s) 916
AoxI GGCC 5 cut(s) 63, 198, 384, 759, 865
ApeKI GCWGC 4 cut(s) 119, 341, 419, 482
ApoI RAATTY 1 cut(s) 289
AspLEI GCGC 3 cut(s) 304, 744, 987
AspS9I GGNCC 2 cut(s) 759, 884
AsuC2I CCSGG 1 cut(s) 285
AsuHPI GGTGA 1 cut(s) 173
AvaII GGWCC 1 cut(s) 884
BanI GGYRCC 1 cut(s) 741
BbrPI CACGTG 1 cut(s) 398
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 3 cut(s) 258, 691, 843
BbvCI CCTCAGC 2 cut(s) 224, 597
BbvI GCAGC 4 cut(s) 106, 328, 431, 469
BccI CCATC 3 cut(s) 314, 494, 677
BceAI ACGGC 3 cut(s) 880, 881, 936
BcgI CGANNNNNNTGC 4 cut(s) 185, 219, 314, 348
BcnI CCSGG 1 cut(s) 285
BcoDI GTCTC 1 cut(s) 468
BfaI CTAG 1 cut(s) 756
BfmI CTRYAG 3 cut(s) 483, 507, 734
BfoI RGCGCY 2 cut(s) 745, 988
BglI GCCNNNNNGGC 1 cut(s) 206
BisI GCNGC 7 cut(s) 66, 120, 207, 267, 342, 420, 483
BlsI GCNGC 7 cut(s) 67, 121, 208, 268, 343, 421, 484
Bme1390I CCNGG 1 cut(s) 285
Bme18I GGWCC 1 cut(s) 884
BmgT120I GGNCC 2 cut(s) 759, 884
BmiI GGNNCC 1 cut(s) 743
BmrFI CCNGG 1 cut(s) 285
BmsI GCATC 4 cut(s) 193, 370, 553, 883
BpiI GAAGAC 1 cut(s) 156
Bpu10I CCTNAGC 2 cut(s) 224, 597
BpuMI CCSGG 1 cut(s) 285
BsaAI YACGTR 1 cut(s) 398
BsaBI GATNNNNATC 1 cut(s) 1013
BsaHI GRCGYC 1 cut(s) 742
BsaJI CCNNGG 1 cut(s) 234
BsaXI ACNNNNNCTCC 2 cut(s) 53, 83
Bsc4I CCNNNNNNNGG 2 cut(s) 599, 874
Bse118I RCCGGY 1 cut(s) 269
Bse1I ACTGG 1 cut(s) 26
Bse3DI GCAATG 2 cut(s) 112, 718
Bse8I GATNNNNATC 1 cut(s) 1013
BseDI CCNNGG 1 cut(s) 234
BseGI GGATG 2 cut(s) 505, 568
BseJI GATNNNNATC 1 cut(s) 1013
BseLI CCNNNNNNNGG 2 cut(s) 599, 874
BseMI GCAATG 2 cut(s) 112, 718
BseMII CTCAG 3 cut(s) 215, 368, 588
BseNI ACTGG 1 cut(s) 26
BseRI GAGGAG 1 cut(s) 68
BseX3I CGGCCG 1 cut(s) 384
BseXI GCAGC 4 cut(s) 106, 328, 431, 469
BsgI GTGCAG 2 cut(s) 929, 985
Bsh1236I CGCG 2 cut(s) 68, 1019
Bsh1285I CGRYCG 1 cut(s) 387
BshFI GGCC 5 cut(s) 65, 200, 386, 761, 867
BshNI GGYRCC 1 cut(s) 741
BsiEI CGRYCG 1 cut(s) 387
BsiHKAI GWGCWC 3 cut(s) 258, 691, 843
BsiSI CCGG 3 cut(s) 270, 285, 387
BslFI GGGAC 3 cut(s) 14, 615, 942
BslI CCNNNNNNNGG 2 cut(s) 599, 874
BsmAI GTCTC 1 cut(s) 468
BsmFI GGGAC 3 cut(s) 14, 615, 942
BsnI GGCC 5 cut(s) 65, 200, 386, 761, 867
Bsp1286I GDGCHC 3 cut(s) 258, 691, 843
Bsp143I GATC 3 cut(s) 584, 769, 1008
Bsp19I CCATGG 1 cut(s) 234
BspACI CCGC 8 cut(s) 18, 59, 66, 88, 206, 209, 267, 344
BspANI GGCC 5 cut(s) 65, 200, 386, 761, 867
BspCNI CTCAG 3 cut(s) 216, 367, 589
BspFNI CGCG 2 cut(s) 68, 1019
BspLI GGNNCC 1 cut(s) 743
BspMAI CTGCAG 1 cut(s) 487
BspT107I GGYRCC 1 cut(s) 741
BsrDI GCAATG 2 cut(s) 112, 718
BsrFI RCCGGY 1 cut(s) 269
BsrI ACTGG 1 cut(s) 26
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 1 cut(s) 234
BssMI GATC 3 cut(s) 584, 769, 1008
BssNI GRCGYC 1 cut(s) 742
BssT1I CCWWGG 1 cut(s) 234
Bst4CI ACNGT 4 cut(s) 245, 443, 541, 918
Bst6I CTCTTC 1 cut(s) 471
BstACI GRCGYC 1 cut(s) 742
BstBAI YACGTR 1 cut(s) 398
BstC8I GCNNGC 3 cut(s) 231, 271, 687
BstDEI CTNAG 3 cut(s) 224, 354, 597
BstDSI CCRYGG 1 cut(s) 234
BstF5I GGATG 2 cut(s) 505, 568
BstFNI CGCG 2 cut(s) 68, 1019
BstH2I RGCGCY 2 cut(s) 745, 988
BstHHI GCGC 3 cut(s) 304, 744, 987
BstKTI GATC 3 cut(s) 587, 772, 1011
BstMAI GTCTC 1 cut(s) 468
BstMBI GATC 3 cut(s) 584, 769, 1008
BstMCI CGRYCG 1 cut(s) 387
BstSCI CCNGG 1 cut(s) 283
BstSFI CTRYAG 3 cut(s) 483, 507, 734
BstUI CGCG 2 cut(s) 68, 1019
BstV1I GCAGC 4 cut(s) 106, 328, 431, 469
BstV2I GAAGAC 1 cut(s) 156
BstZI CGGCCG 1 cut(s) 384
BsuRI GGCC 5 cut(s) 65, 200, 386, 761, 867
BtgI CCRYGG 1 cut(s) 234
BtsCI GGATG 2 cut(s) 505, 568
BtsI GCAGTG 2 cut(s) 579, 1002
BtsIMutI CAGTG 2 cut(s) 579, 1002
Cac8I GCNNGC 3 cut(s) 231, 271, 687
CaiI CAGNNNCTG 1 cut(s) 916
CfoI GCGC 3 cut(s) 304, 744, 987
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 2 cut(s) 759, 884
CseI GACGC 1 cut(s) 57
CspCI CAANNNNNGTGG 4 cut(s) 384, 419, 555, 590
CviAII CATG 2 cut(s) 235, 326
DdeI CTNAG 3 cut(s) 224, 354, 597
DinI GGCGCC 1 cut(s) 743
DpnI GATC 3 cut(s) 586, 771, 1010
DpnII GATC 3 cut(s) 584, 769, 1008
EaeI YGGCCR 1 cut(s) 384
EagI CGGCCG 1 cut(s) 384
Eam1104I CTCTTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 471
EciI GGCGGA 2 cut(s) 74, 224
EclXI CGGCCG 1 cut(s) 384
Eco130I CCWWGG 1 cut(s) 234
Eco47I GGWCC 1 cut(s) 884
Eco52I CGGCCG 1 cut(s) 384
Eco57I CTGAAG 1 cut(s) 656
Eco72I CACGTG 1 cut(s) 398
EcoO109I RGGNCCY 1 cut(s) 759
EcoT14I CCWWGG 1 cut(s) 234
EgeI GGCGCC 1 cut(s) 743
EheI GGCGCC 1 cut(s) 743
ErhI CCWWGG 1 cut(s) 234
FaeI CATG 2 cut(s) 238, 329
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FaqI GGGAC 3 cut(s) 14, 615, 942
FatI CATG 2 cut(s) 234, 325
FauI CCCGC 1 cut(s) 81
Fnu4HI GCNGC 7 cut(s) 66, 120, 207, 267, 342, 420, 483
FokI GGATG 2 cut(s) 512, 575
Fsp4HI GCNGC 7 cut(s) 66, 120, 207, 267, 342, 420, 483
FspAI RTGCGCAY 1 cut(s) 303
FspBI CTAG 1 cut(s) 756
FspI TGCGCA 1 cut(s) 303
GlaI GCGC 3 cut(s) 303, 743, 986
GluI GCNGC 7 cut(s) 66, 120, 207, 267, 342, 420, 483
HaeII RGCGCY 2 cut(s) 745, 988
HaeIII GGCC 5 cut(s) 65, 200, 386, 761, 867
HapII CCGG 3 cut(s) 270, 285, 387
HgaI GACGC 1 cut(s) 57
HhaI GCGC 3 cut(s) 304, 744, 987
Hin1I GRCGYC 1 cut(s) 742
Hin1II CATG 2 cut(s) 238, 329
Hin6I GCGC 3 cut(s) 302, 742, 985
HinP1I GCGC 3 cut(s) 302, 742, 985
HinfI GANTC 2 cut(s) 98, 461
HpaII CCGG 3 cut(s) 270, 285, 387
HphI GGTGA 1 cut(s) 173
Hpy188I TCNGA 4 cut(s) 357, 366, 450, 699
Hpy188III TCNNGA 2 cut(s) 31, 473
Hpy99I CGWCG 3 cut(s) 37, 866, 957
HpyAV CCTTC 5 cut(s) 86, 152, 631, 694, 916
HpyCH4III ACNGT 4 cut(s) 245, 443, 541, 918
HpyCH4IV ACGT 1 cut(s) 397
HpyF3I CTNAG 3 cut(s) 224, 354, 597
HpySE526I ACGT 1 cut(s) 397
Hsp92I GRCGYC 1 cut(s) 742
Hsp92II CATG 2 cut(s) 238, 329
HspAI GCGC 3 cut(s) 302, 742, 985
KasI GGCGCC 1 cut(s) 741
KroI GCCGGC 1 cut(s) 269
KroNI GCCGGC 1 cut(s) 271
Kzo9I GATC 3 cut(s) 584, 769, 1008
LmnI GCTCC 2 cut(s) 211, 682
Lsp1109I GCAGC 4 cut(s) 106, 328, 431, 469
LweI GCATC 4 cut(s) 193, 370, 553, 883
MaeI CTAG 1 cut(s) 756
MaeII ACGT 1 cut(s) 397
MalI GATC 3 cut(s) 586, 771, 1010
MboI GATC 3 cut(s) 584, 769, 1008
MboII GAAGA 5 cut(s) 152, 155, 161, 458, 461
MhlI GDGCHC 3 cut(s) 258, 691, 843
MluCI AATT 3 cut(s) 289, 849, 879
Mly113I GGCGCC 1 cut(s) 742
MlyI GAGTC 1 cut(s) 470
MmeI TCCRAC 1 cut(s) 387
MreI CGCCGGCG 1 cut(s) 269
MroNI GCCGGC 1 cut(s) 269
MslI CAYNNNNRTG 1 cut(s) 374
MspA1I CMGCKG 2 cut(s) 119, 512
MspI CCGG 3 cut(s) 270, 285, 387
MspR9I CCNGG 1 cut(s) 285
MvnI CGCG 2 cut(s) 68, 1019
NaeI GCCGGC 1 cut(s) 271
NarI GGCGCC 1 cut(s) 742
NciI CCSGG 1 cut(s) 285
NcoI CCATGG 1 cut(s) 234
NdeII GATC 3 cut(s) 584, 769, 1008
NgoMIV GCCGGC 1 cut(s) 269
NlaIII CATG 2 cut(s) 238, 329
NlaIV GGNNCC 1 cut(s) 743
NsbI TGCGCA 1 cut(s) 303
OliI CACNNNNGTG 1 cut(s) 374
PdiI GCCGGC 1 cut(s) 271
PfeI GAWTC 1 cut(s) 98
PfoI TCCNGGA 1 cut(s) 283
PkrI GCNGC 7 cut(s) 67, 121, 208, 268, 343, 421, 484
PleI GAGTC 1 cut(s) 469
PluTI GGCGCC 1 cut(s) 745
PmaCI CACGTG 1 cut(s) 398
PmlI CACGTG 1 cut(s) 398
PpsI GAGTC 1 cut(s) 469
Ppu21I YACGTR 1 cut(s) 398
PspCI CACGTG 1 cut(s) 398
PspN4I GGNNCC 1 cut(s) 743
PspPI GGNCC 2 cut(s) 759, 884
PstI CTGCAG 1 cut(s) 487
PstNI CAGNNNCTG 1 cut(s) 916
PvuII CAGCTG 2 cut(s) 119, 512
RseI CAYNNNNRTG 1 cut(s) 374
SatI GCNGC 7 cut(s) 66, 120, 207, 267, 342, 420, 483
Sau3AI GATC 3 cut(s) 584, 769, 1008
Sau96I GGNCC 2 cut(s) 759, 884
SchI GAGTC 1 cut(s) 470
ScrFI CCNGG 1 cut(s) 285
SduI GDGCHC 3 cut(s) 258, 691, 843
SetI ASST 9 cut(s) 121, 163, 334, 400, 514, 789, 828, 937, 973
SfaNI GCATC 4 cut(s) 193, 370, 553, 883
SfcI CTRYAG 3 cut(s) 483, 507, 734
SfoI GGCGCC 1 cut(s) 743
SgrAI CRCCGGYG 1 cut(s) 269
SinI GGWCC 1 cut(s) 884
SmiMI CAYNNNNRTG 1 cut(s) 374
Sse9I AATT 3 cut(s) 289, 849, 879
SsiI CCGC 8 cut(s) 18, 59, 66, 88, 206, 209, 267, 344
SspDI GGCGCC 1 cut(s) 741
SspMI CTAG 1 cut(s) 756
StyD4I CCNGG 1 cut(s) 283
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 4 cut(s) 245, 443, 541, 918
TaiI ACGT 1 cut(s) 400
TaqI TCGA 3 cut(s) 195, 334, 472
TasI AATT 3 cut(s) 289, 849, 879
TauI GCSGC 3 cut(s) 68, 209, 269
TfiI GAWTC 1 cut(s) 98
TscAI CASTG 2 cut(s) 579, 1009
TseI GCWGC 4 cut(s) 119, 341, 419, 482
TspDTI ATGAA 4 cut(s) 162, 636, 803, 835
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 2 cut(s) 579, 1009
VpaK11BI GGWCC 1 cut(s) 884
XapI RAATTY 1 cut(s) 289
XspI CTAG 1 cut(s) 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.