pycom07g22750

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007067
Physical Location & Seq
Forward (+)
23956481 .. 23958605
2125 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1161 bp
ATGGACCTGAAATCAAATCATGCTTCTCCCGTTCTTGCTGATCCTCCACCCTTGAGCAAGTCAAGACTTGGTGGCGTCCATACCAGCCTGTTACCGTATTCAACTCCAGGAGCAGCTTTCCCTGGAGGTCTATACCTAACAATCCCTAGGAAAAAGAATGCATTACTTGATGACGTTCGCTCTAGCTCTTGGCTCGATGCCATGAAAGCTTCATCGCCTCCTCACAGAAAGATAACGAAGGATGTTAACAATGAGTCTGGGGCAACAGATGCTGATATATCTTATCACACATGGATGGTCAAGTATCCATCCGCACTTACATCTTTTGAGCAAATCTCTAATTATGCCAAAGGCAAGAGAATAGCATTGTTTCTTGATTATGACGGGACTCTTTCACCAATTGTAGATAACCCTGACTGTGCCTTTATGTCTGATGCCATGCGTGCATCCGTAAGAAAGGTAGCAAAACATTTCCCAACAGCAATTATCAGTGGAAGAAGCCATGACAAGGTATACGAGTTTGTAAAACTAAATGAACTCTATTATGCGGGTAGTCATGGGATGGACATAATGGCCCCGGTTATACAAAGTATAAACGATGACCAGAGAAACTGTTTTAAGTCAACTGACAAAAAGGGTAAGGCGGTTAATTTGTTCCAGCCTGCAGCTGAATTTTTACCGACGATTAGAGAGGTATTTAAATTGCTCGTTGAAAGTACGAAAGACATTGAAGGAGCTATAGTTGAGGATAATAAGTTCTGTGTCTCTGTACATTACCGCAATGTAGATGAGAAGAGTTGGCCTGCGGTTCTGCAATGTGTCCATGAAATTCTTAAAGACTACCCTCGTTTGCGCTTGACTCATGGGCGAAAGGTTTTAGAGGTTCGACCTACAATAAACTGGGATAAAGGGAAGGCCGTCACGTTTTTACTCGAATCTCTTGGACTAAGTAATTGCGAAGATGTTCTTCCAATATATATTGGCGACGACCGCACAGATGAAGATGCATTCAAGGTCTTGAGGGAAGGAAACCAAGGTTACGGCATTTTAGTCTCGGCCGAGCCCAAGGACAGCAATGCATTTTACTCTCTCCGGGATCCCTCGGAGGTAATGGAGTTTCTTAAGTCACTGGTTATATGGAGGAAGTCAAGTGCCCTATAA

Protein Analysis

387

Amino Acids

43.1

Weight (kDa)

7.14

Isoelectric Point (pI)

34.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 124 - 368 2.7e-72 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 513
AciI CCGC 6 cut(s) 312, 548, 644, 778, 806, 991
AclWI GGATC 3 cut(s) 35, 1091, 1104
AcoI YGGCCR 1 cut(s) 1056
AcsI RAATTY 2 cut(s) 671, 828
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 2 cut(s) 718, 771
AfiI CCNNNNNNNGG 1 cut(s) 508
AflII CTTAAG 1 cut(s) 1121
AgsI TTSAA 4 cut(s) 102, 713, 731, 1012
AjnI CCWGG 2 cut(s) 106, 121
AloI GAACNNNNNNTCC 2 cut(s) 740, 772
AluBI AGCT 5 cut(s) 116, 186, 209, 668, 737
AluI AGCT 5 cut(s) 116, 186, 209, 668, 737
Alw26I GTCTC 2 cut(s) 769, 1057
AlwI GGATC 3 cut(s) 35, 1091, 1104
AlwNI CAGNNNCTG 1 cut(s) 272
AoxI GGCC 4 cut(s) 573, 800, 915, 1056
ApeKI GCWGC 2 cut(s) 113, 665
ApoI RAATTY 2 cut(s) 671, 828
Asp700I GAANNNNTTC 1 cut(s) 963
AspA2I CCTAGG 1 cut(s) 146
AspLEI GCGC 1 cut(s) 855
AspS9I GGNCC 2 cut(s) 4, 574
AsuC2I CCSGG 2 cut(s) 578, 1094
AsuHPI GGTGA 1 cut(s) 387
AvaII GGWCC 1 cut(s) 4
AvrII CCTAGG 1 cut(s) 146
BaeGI GKGCMC 1 cut(s) 1156
BamHI GGATCC 1 cut(s) 1096
BanII GRGCYC 1 cut(s) 1065
BbvI GCAGC 2 cut(s) 125, 677
BccI CCATC 3 cut(s) 289, 316, 556
BceAI ACGGC 2 cut(s) 902, 1057
BciT130I CCWGG 2 cut(s) 108, 123
BciVI GTATCC 1 cut(s) 315
BcnI CCSGG 2 cut(s) 578, 1094
BcoDI GTCTC 2 cut(s) 769, 1057
BfaI CTAG 2 cut(s) 147, 183
BfmI CTRYAG 2 cut(s) 663, 738
BfrI CTTAAG 1 cut(s) 1121
BfuI GTATCC 1 cut(s) 315
BisI GCNGC 2 cut(s) 114, 666
BlnI CCTAGG 1 cut(s) 146
BlsI GCNGC 2 cut(s) 115, 667
Bme1390I CCNGG 4 cut(s) 108, 123, 578, 1094
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 2 cut(s) 4, 574
BmiI GGNNCC 2 cut(s) 576, 1098
BmrFI CCNGG 4 cut(s) 108, 123, 578, 1094
BmrI ACTGGG 1 cut(s) 910
BmsI GCATC 5 cut(s) 187, 259, 424, 455, 994
BmuI ACTGGG 1 cut(s) 910
BplI GAGNNNNNCTC 2 cut(s) 320, 352
BpmI CTGGAG 2 cut(s) 90, 144
BpuEI CTTGAG 2 cut(s) 73, 1039
BpuMI CCSGG 2 cut(s) 578, 1094
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 6 cut(s) 121, 146, 576, 1033, 1065, 1101
BsaXI ACNNNNNCTCC 2 cut(s) 726, 756
Bsc4I CCNNNNNNNGG 1 cut(s) 508
Bse1I ACTGG 2 cut(s) 905, 1134
Bse3DI GCAATG 3 cut(s) 787, 821, 1081
BseBI CCWGG 2 cut(s) 108, 123
BseDI CCNNGG 6 cut(s) 121, 146, 576, 1033, 1065, 1101
BseGI GGATG 5 cut(s) 247, 300, 308, 446, 567
BseLI CCNNNNNNNGG 1 cut(s) 508
BseMI GCAATG 3 cut(s) 787, 821, 1081
BseNI ACTGG 2 cut(s) 905, 1134
BseRI GAGGAG 1 cut(s) 210
BseSI GKGCMC 1 cut(s) 1156
BseX3I CGGCCG 1 cut(s) 1056
BseXI GCAGC 2 cut(s) 125, 677
Bsh1285I CGRYCG 2 cut(s) 991, 1059
BshFI GGCC 4 cut(s) 575, 802, 917, 1058
BsiEI CGRYCG 2 cut(s) 991, 1059
BsiSI CCGG 2 cut(s) 578, 1093
BslFI GGGAC 1 cut(s) 400
BslI CCNNNNNNNGG 1 cut(s) 508
BsmAI GTCTC 2 cut(s) 769, 1057
BsmFI GGGAC 1 cut(s) 400
BsmI GAATGC 2 cut(s) 163, 1007
BsnI GGCC 4 cut(s) 575, 802, 917, 1058
Bsp1286I GDGCHC 2 cut(s) 1065, 1156
Bsp1407I TGTACA 1 cut(s) 769
Bsp143I GATC 2 cut(s) 40, 1096
BspACI CCGC 6 cut(s) 312, 548, 644, 778, 806, 991
BspANI GGCC 4 cut(s) 575, 802, 917, 1058
BspLI GGNNCC 2 cut(s) 576, 1098
BspMAI CTGCAG 1 cut(s) 667
BspPI GGATC 3 cut(s) 35, 1091, 1104
BspTI CTTAAG 1 cut(s) 1121
BsrDI GCAATG 3 cut(s) 787, 821, 1081
BsrGI TGTACA 1 cut(s) 769
BsrI ACTGG 2 cut(s) 905, 1134
BssECI CCNNGG 6 cut(s) 121, 146, 576, 1033, 1065, 1101
BssMI GATC 2 cut(s) 40, 1096
BssNAI GTATAC 1 cut(s) 514
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 3 cut(s) 146, 1033, 1065
Bst1107I GTATAC 1 cut(s) 514
Bst2UI CCWGG 2 cut(s) 108, 123
Bst4CI ACNGT 3 cut(s) 96, 419, 614
Bst6I CTCTTC 1 cut(s) 788
BstACI GRCGYC 1 cut(s) 75
BstAFI CTTAAG 1 cut(s) 1121
BstAPI GCANNNNNTGC 1 cut(s) 269
BstAUI TGTACA 1 cut(s) 769
BstC8I GCNNGC 3 cut(s) 444, 663, 804
BstDEI CTNAG 1 cut(s) 947
BstF5I GGATG 5 cut(s) 247, 300, 308, 446, 567
BstHHI GCGC 1 cut(s) 855
BstKTI GATC 2 cut(s) 43, 1099
BstMAI GTCTC 2 cut(s) 769, 1057
BstMBI GATC 2 cut(s) 40, 1096
BstMCI CGRYCG 2 cut(s) 991, 1059
BstMWI GCNNNNNNNGC 4 cut(s) 206, 269, 443, 990
BstNI CCWGG 2 cut(s) 108, 123
BstSCI CCNGG 4 cut(s) 106, 121, 576, 1092
BstSFI CTRYAG 2 cut(s) 663, 738
BstSLI GKGCMC 1 cut(s) 1156
BstV1I GCAGC 2 cut(s) 125, 677
BstX2I RGATCY 1 cut(s) 1096
BstYI RGATCY 1 cut(s) 1096
BstZ17I GTATAC 1 cut(s) 514
BstZI CGGCCG 1 cut(s) 1056
BsuI GTATCC 1 cut(s) 315
BsuRI GGCC 4 cut(s) 575, 802, 917, 1058
BtgZI GCGATG 1 cut(s) 198
BtsCI GGATG 5 cut(s) 247, 300, 308, 446, 567
BtsIMutI CAGTG 2 cut(s) 496, 1127
Cac8I GCNNGC 3 cut(s) 444, 663, 804
CaiI CAGNNNCTG 1 cut(s) 272
CfoI GCGC 1 cut(s) 855
Cfr13I GGNCC 2 cut(s) 4, 574
CseI GACGC 1 cut(s) 64
Csp6I GTAC 2 cut(s) 717, 770
CviAII CATG 8 cut(s) 20, 202, 291, 439, 503, 557, 824, 863
CviQI GTAC 2 cut(s) 717, 770
DdeI CTNAG 1 cut(s) 947
DpnI GATC 2 cut(s) 42, 1098
DpnII GATC 2 cut(s) 40, 1096
DraI TTTAAA 1 cut(s) 700
EaeI YGGCCR 1 cut(s) 1056
EagI CGGCCG 1 cut(s) 1056
Eam1104I CTCTTC 1 cut(s) 788
EarI CTCTTC 1 cut(s) 788
EclXI CGGCCG 1 cut(s) 1056
Eco130I CCWWGG 3 cut(s) 146, 1033, 1065
Eco24I GRGCYC 1 cut(s) 1065
Eco47I GGWCC 1 cut(s) 4
Eco52I CGGCCG 1 cut(s) 1056
EcoRII CCWGG 2 cut(s) 106, 121
EcoT14I CCWWGG 3 cut(s) 146, 1033, 1065
EcoT22I ATGCAT 3 cut(s) 163, 1009, 1081
EcoT38I GRGCYC 1 cut(s) 1065
ErhI CCWWGG 3 cut(s) 146, 1033, 1065
FaeI CATG 8 cut(s) 23, 205, 294, 442, 506, 560, 827, 866
FalI AAGNNNNNCTT 2 cut(s) 951, 983
FaqI GGGAC 1 cut(s) 400
FatI CATG 8 cut(s) 19, 201, 290, 438, 502, 556, 823, 862
FauI CCCGC 1 cut(s) 541
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 2 cut(s) 114, 666
FokI GGATG 5 cut(s) 254, 295, 307, 433, 574
FriOI GRGCYC 1 cut(s) 1065
Fsp4HI GCNGC 2 cut(s) 114, 666
FspBI CTAG 2 cut(s) 147, 183
GlaI GCGC 1 cut(s) 854
GluI GCNGC 2 cut(s) 114, 666
GsuI CTGGAG 2 cut(s) 90, 144
HaeIII GGCC 4 cut(s) 575, 802, 917, 1058
HapII CCGG 2 cut(s) 578, 1093
HgaI GACGC 1 cut(s) 64
HhaI GCGC 1 cut(s) 855
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 8 cut(s) 23, 205, 294, 442, 506, 560, 827, 866
Hin6I GCGC 1 cut(s) 853
HinP1I GCGC 1 cut(s) 853
HincII GTYRAC 2 cut(s) 247, 624
HindII GTYRAC 2 cut(s) 247, 624
HindIII AAGCTT 1 cut(s) 207
HinfI GANTC 4 cut(s) 254, 388, 859, 935
HpaI GTTAAC 1 cut(s) 247
HpaII CCGG 2 cut(s) 578, 1093
HphI GGTGA 1 cut(s) 387
Hpy166II GTNNAC 3 cut(s) 247, 514, 624
Hpy188I TCNGA 2 cut(s) 433, 1105
Hpy188III TCNNGA 3 cut(s) 63, 374, 1018
Hpy8I GTNNAC 3 cut(s) 247, 514, 624
Hpy99I CGWCG 2 cut(s) 685, 989
HpyAV CCTTC 4 cut(s) 232, 725, 907, 1019
HpyCH4III ACNGT 3 cut(s) 96, 419, 614
HpyCH4IV ACGT 2 cut(s) 174, 923
HpyCH4V TGCA 6 cut(s) 161, 446, 665, 814, 1007, 1079
HpyF10VI GCNNNNNNNGC 4 cut(s) 206, 269, 443, 990
HpyF3I CTNAG 1 cut(s) 947
HpySE526I ACGT 2 cut(s) 174, 923
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 8 cut(s) 23, 205, 294, 442, 506, 560, 827, 866
HspAI GCGC 1 cut(s) 853
KspAI GTTAAC 1 cut(s) 247
Kzo9I GATC 2 cut(s) 40, 1096
LmnI GCTCC 2 cut(s) 110, 734
Lsp1109I GCAGC 2 cut(s) 125, 677
LweI GCATC 5 cut(s) 187, 259, 424, 455, 994
MaeI CTAG 2 cut(s) 147, 183
MaeII ACGT 2 cut(s) 174, 923
MaeIII GTNAC 4 cut(s) 90, 919, 1037, 1125
MalI GATC 2 cut(s) 42, 1098
MboI GATC 2 cut(s) 40, 1096
MboII GAAGA 5 cut(s) 507, 805, 959, 971, 1013
MfeI CAATTG 1 cut(s) 399
MflI RGATCY 1 cut(s) 1096
MhlI GDGCHC 2 cut(s) 1065, 1156
MluCI AATT 8 cut(s) 340, 399, 483, 649, 671, 701, 828, 952
MlyI GAGTC 3 cut(s) 263, 382, 853
Mph1103I ATGCAT 3 cut(s) 163, 1009, 1081
MroXI GAANNNNTTC 1 cut(s) 963
MseI TTAA 6 cut(s) 246, 618, 648, 699, 834, 1122
MslI CAYNNNNRTG 1 cut(s) 293
MspA1I CMGCKG 1 cut(s) 668
MspCI CTTAAG 1 cut(s) 1121
MspI CCGG 2 cut(s) 578, 1093
MspR9I CCNGG 4 cut(s) 108, 123, 578, 1094
MunI CAATTG 1 cut(s) 399
Mva1269I GAATGC 2 cut(s) 163, 1007
MvaI CCWGG 2 cut(s) 108, 123
MwoI GCNNNNNNNGC 4 cut(s) 206, 269, 443, 990
NciI CCSGG 2 cut(s) 578, 1094
NdeII GATC 2 cut(s) 40, 1096
NlaIII CATG 8 cut(s) 23, 205, 294, 442, 506, 560, 827, 866
NlaIV GGNNCC 2 cut(s) 576, 1098
NmeAIII GCCGAG 2 cut(s) 1034, 1084
NmuCI GTSAC 2 cut(s) 919, 1125
NsiI ATGCAT 3 cut(s) 163, 1009, 1081
PctI GAATGC 2 cut(s) 163, 1007
PdmI GAANNNNTTC 1 cut(s) 963
PfeI GAWTC 1 cut(s) 935
PfoI TCCNGGA 2 cut(s) 106, 1092
PkrI GCNGC 2 cut(s) 115, 667
PleI GAGTC 3 cut(s) 262, 382, 853
PpsI GAGTC 3 cut(s) 262, 382, 853
Psp6I CCWGG 2 cut(s) 106, 121
PspGI CCWGG 2 cut(s) 106, 121
PspN4I GGNNCC 2 cut(s) 576, 1098
PspPI GGNCC 2 cut(s) 4, 574
PstI CTGCAG 1 cut(s) 667
PstNI CAGNNNCTG 1 cut(s) 272
PsuI RGATCY 1 cut(s) 1096
PvuII CAGCTG 1 cut(s) 668
RsaI GTAC 2 cut(s) 718, 771
RsaNI GTAC 2 cut(s) 717, 770
RseI CAYNNNNRTG 1 cut(s) 293
SaqAI TTAA 6 cut(s) 246, 618, 648, 699, 834, 1122
SatI GCNGC 2 cut(s) 114, 666
Sau3AI GATC 2 cut(s) 40, 1096
Sau96I GGNCC 2 cut(s) 4, 574
SchI GAGTC 3 cut(s) 263, 382, 853
ScrFI CCNGG 4 cut(s) 108, 123, 578, 1094
SduI GDGCHC 2 cut(s) 1065, 1156
SfaNI GCATC 5 cut(s) 187, 259, 424, 455, 994
SfcI CTRYAG 2 cut(s) 663, 738
SinI GGWCC 1 cut(s) 4
SmiI ATTTAAAT 1 cut(s) 700
SmiMI CAYNNNNRTG 1 cut(s) 293
SmlI CTYRAG 3 cut(s) 52, 1018, 1121
SmoI CTYRAG 3 cut(s) 52, 1018, 1121
Sse9I AATT 8 cut(s) 340, 399, 483, 649, 671, 701, 828, 952
SsiI CCGC 6 cut(s) 312, 548, 644, 778, 806, 991
SspMI CTAG 2 cut(s) 147, 183
StyD4I CCNGG 4 cut(s) 106, 121, 576, 1092
StyI CCWWGG 3 cut(s) 146, 1033, 1065
SwaI ATTTAAAT 1 cut(s) 700
TaaI ACNGT 3 cut(s) 96, 419, 614
TaiI ACGT 2 cut(s) 177, 926
TaqI TCGA 3 cut(s) 195, 886, 933
TasI AATT 8 cut(s) 340, 399, 483, 649, 671, 701, 828, 952
TatI WGTACW 1 cut(s) 769
TfiI GAWTC 1 cut(s) 935
Tru1I TTAA 6 cut(s) 246, 618, 648, 699, 834, 1122
Tru9I TTAA 6 cut(s) 246, 618, 648, 699, 834, 1122
TscAI CASTG 2 cut(s) 496, 1134
TseFI GTSAC 2 cut(s) 919, 1125
TseI GCWGC 2 cut(s) 113, 665
Tsp45I GTSAC 2 cut(s) 919, 1125
TspDTI ATGAA 5 cut(s) 201, 218, 549, 840, 1014
TspGWI ACGGA 1 cut(s) 439
TspRI CASTG 2 cut(s) 496, 1134
Vha464I CTTAAG 1 cut(s) 1121
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 2 cut(s) 671, 828
XmaJI CCTAGG 1 cut(s) 146
XmiI GTMKAC 1 cut(s) 513
XmnI GAANNNNTTC 1 cut(s) 963
XspI CTAG 2 cut(s) 147, 183
Zsp2I ATGCAT 3 cut(s) 163, 1009, 1081
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.