pycom07g25970

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005995
Physical Location & Seq
Reverse (-)
26129016 .. 26131604
2589 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 819 bp
ATGATCTGCAAAAGTTACATTAGGAGTGTGATTGTTCAAGCCTGGAATATCGTAACGTCTACGAAGGCAAGGGTGCATTTCAACAATACTGATAGAATTTGCGTGCTTGCCAATTCGTGGAACCGAGCATTTAGGTCGAGTTTGAGGATGATGGTTGATACAGGTGCGACGGATGAACAAGGATCCGTTTTTGATGTGCTGCCTGACAAAGATGAGGATGGTGAATATACCAGTGGAGGGTGCAAAAGCGAAGGTGGAGAACTGAGTTGCGGTTATTCGAGTTTCAGGGGAAAAAGAGCAACTATGGAGGATTTTTATGATGTTAAAATGTCCAAAATTGAAGGGCAAACGGTCTGCCTGTTTGGAATTTTCGATGGTCACGGCGGTTCTCGTGCTGCTCAGTATTTGAAGGAGCATCTATTTGAGAATCTCATGAAGCATCCACAATTCATGACAGACACTAAGTTAGCTATTAGTGAATCGTATCAACAGACCGATGCAGACTTTTTGGATTCTGAAAGAGATATATACCGGGATGATGGTTCTACTGCTTCAACAGCAGTCTTGGTTGGTAACCATCTATATGTTGCTAATGTTGGAGATTCACGTACAGTAATATCAAAGGCAGGAAAAGCTATTGCTTTATCCGAGGATCATAAACCAAATCGAAGTGATGAGAGGAAGAGAATTGAGAGTGCTGGGGGTTTTGTAATGTGGACAGGCACTTGGAGAGTAGGCGGTGTGCTGGCCATGTCTCGGGCTTTTGGGAACCGCATGTTGAAGCAATATGTTGTTGCAGAACCTGAGATCGAGACCTAG

Protein Analysis

273

Amino Acids

30.27

Weight (kDa)

5.91

Isoelectric Point (pI)

39.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 91 - 271 3.1e-46 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 117
AccI GTMKAC 1 cut(s) 59
AciI CCGC 4 cut(s) 270, 384, 738, 772
AclWI GGATC 3 cut(s) 177, 190, 660
AcoI YGGCCR 1 cut(s) 747
AcsI RAATTY 2 cut(s) 96, 366
AfaI GTAC 1 cut(s) 610
AfiI CCNNNNNNNGG 3 cut(s) 117, 237, 756
AgsI TTSAA 6 cut(s) 38, 82, 341, 409, 555, 781
AjnI CCWGG 1 cut(s) 41
AluBI AGCT 2 cut(s) 470, 635
AluI AGCT 2 cut(s) 470, 635
Alw26I GTCTC 2 cut(s) 759, 806
AlwI GGATC 3 cut(s) 177, 190, 660
AlwNI CAGNNNCTG 1 cut(s) 803
Ama87I CYCGRG 1 cut(s) 756
AoxI GGCC 1 cut(s) 747
ApeKI GCWGC 2 cut(s) 199, 395
ApoI RAATTY 2 cut(s) 96, 366
AsuC2I CCSGG 1 cut(s) 533
AsuHPI GGTGA 1 cut(s) 233
AvaI CYCGRG 1 cut(s) 756
BalI TGGCCA 1 cut(s) 749
BamHI GGATCC 1 cut(s) 182
BauI CACGAG 1 cut(s) 390
BbvI GCAGC 2 cut(s) 186, 382
BccI CCATC 5 cut(s) 145, 212, 368, 533, 585
BceAI ACGGC 1 cut(s) 397
BcgI CGANNNNNNTGC 2 cut(s) 117, 151
BciT130I CCWGG 1 cut(s) 43
BcnI CCSGG 1 cut(s) 533
BcoDI GTCTC 2 cut(s) 759, 806
BfaI CTAG 1 cut(s) 817
BisI GCNGC 2 cut(s) 200, 396
BlsI GCNGC 2 cut(s) 201, 397
Bme1390I CCNGG 2 cut(s) 43, 533
BmeT110I CYCGRG 1 cut(s) 756
BmiI GGNNCC 3 cut(s) 122, 184, 770
BmrFI CCNGG 2 cut(s) 43, 533
BmsI GCATC 3 cut(s) 424, 448, 487
BpuMI CCSGG 1 cut(s) 533
BsaAI YACGTR 1 cut(s) 608
BsaI GGTCTC 1 cut(s) 806
BsaJI CCNNGG 1 cut(s) 648
Bsc4I CCNNNNNNNGG 3 cut(s) 117, 237, 756
Bse1I ACTGG 1 cut(s) 231
BseBI CCWGG 1 cut(s) 43
BseDI CCNNGG 1 cut(s) 648
BseGI GGATG 5 cut(s) 153, 178, 223, 439, 541
BseLI CCNNNNNNNGG 3 cut(s) 117, 237, 756
BseMII CTCAG 3 cut(s) 254, 413, 795
BseNI ACTGG 1 cut(s) 231
BseXI GCAGC 2 cut(s) 186, 382
BseYI CCCAGC 1 cut(s) 698
BshFI GGCC 1 cut(s) 749
BsiHKCI CYCGRG 1 cut(s) 756
BsiSI CCGG 1 cut(s) 532
BslI CCNNNNNNNGG 3 cut(s) 117, 237, 756
BsmAI GTCTC 2 cut(s) 759, 806
BsnI GGCC 1 cut(s) 749
Bso31I GGTCTC 1 cut(s) 806
BsoBI CYCGRG 1 cut(s) 756
Bsp143I GATC 4 cut(s) 3, 182, 652, 807
BspACI CCGC 4 cut(s) 270, 384, 738, 772
BspANI GGCC 1 cut(s) 749
BspCNI CTCAG 3 cut(s) 255, 412, 796
BspHI TCATGA 2 cut(s) 432, 450
BspLI GGNNCC 3 cut(s) 122, 184, 770
BspPI GGATC 3 cut(s) 177, 190, 660
BspTNI GGTCTC 1 cut(s) 806
BsrI ACTGG 1 cut(s) 231
BssECI CCNNGG 1 cut(s) 648
BssMI GATC 4 cut(s) 3, 182, 652, 807
BssSI CACGAG 1 cut(s) 390
Bst2BI CACGAG 1 cut(s) 390
Bst2UI CCWGG 1 cut(s) 43
Bst4CI ACNGT 2 cut(s) 352, 613
Bst6I CTCTTC 1 cut(s) 677
BstBAI YACGTR 1 cut(s) 608
BstC8I GCNNGC 3 cut(s) 104, 108, 747
BstDEI CTNAG 4 cut(s) 263, 399, 462, 804
BstEII GGTNACC 1 cut(s) 572
BstF5I GGATG 5 cut(s) 153, 178, 223, 439, 541
BstKTI GATC 4 cut(s) 6, 185, 655, 810
BstMAI GTCTC 2 cut(s) 759, 806
BstMBI GATC 4 cut(s) 3, 182, 652, 807
BstMWI GCNNNNNNNGC 2 cut(s) 557, 632
BstNI CCWGG 1 cut(s) 43
BstNSI RCATGY 1 cut(s) 778
BstPI GGTNACC 1 cut(s) 572
BstSCI CCNGG 2 cut(s) 41, 531
BstV1I GCAGC 2 cut(s) 186, 382
BstX2I RGATCY 1 cut(s) 182
BstYI RGATCY 1 cut(s) 182
BsuRI GGCC 1 cut(s) 749
BtsCI GGATG 5 cut(s) 153, 178, 223, 439, 541
BtsIMutI CAGTG 1 cut(s) 238
Cac8I GCNNGC 3 cut(s) 104, 108, 747
CaiI CAGNNNCTG 1 cut(s) 803
CciI TCATGA 2 cut(s) 432, 450
Csp6I GTAC 1 cut(s) 609
CviAII CATG 4 cut(s) 433, 451, 751, 775
CviJI RGCY 5 cut(s) 41, 470, 635, 749, 761
CviKI_1 RGCY 5 cut(s) 41, 470, 635, 749, 761
CviQI GTAC 1 cut(s) 609
DdeI CTNAG 4 cut(s) 263, 399, 462, 804
DpnI GATC 4 cut(s) 5, 184, 654, 809
DpnII GATC 4 cut(s) 3, 182, 652, 807
EaeI YGGCCR 1 cut(s) 747
Eam1104I CTCTTC 1 cut(s) 677
EarI CTCTTC 1 cut(s) 677
Eco31I GGTCTC 1 cut(s) 806
Eco88I CYCGRG 1 cut(s) 756
Eco91I GGTNACC 1 cut(s) 572
EcoO65I GGTNACC 1 cut(s) 572
EcoRII CCWGG 1 cut(s) 41
FaeI CATG 4 cut(s) 436, 454, 754, 778
FatI CATG 4 cut(s) 432, 450, 750, 774
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 2 cut(s) 200, 396
FokI GGATG 5 cut(s) 160, 185, 230, 426, 548
Fsp4HI GCNGC 2 cut(s) 200, 396
FspBI CTAG 1 cut(s) 817
GluI GCNGC 2 cut(s) 200, 396
GsaI CCCAGC 1 cut(s) 702
HaeIII GGCC 1 cut(s) 749
HapII CCGG 1 cut(s) 532
Hin1II CATG 4 cut(s) 436, 454, 754, 778
HinfI GANTC 4 cut(s) 427, 479, 512, 602
HpaII CCGG 1 cut(s) 532
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 2 cut(s) 60, 717
Hpy188I TCNGA 2 cut(s) 517, 649
Hpy188III TCNNGA 3 cut(s) 433, 451, 811
Hpy8I GTNNAC 2 cut(s) 60, 717
Hpy99I CGWCG 1 cut(s) 172
HpyAV CCTTC 4 cut(s) 58, 245, 335, 403
HpyCH4III ACNGT 2 cut(s) 352, 613
HpyCH4IV ACGT 2 cut(s) 56, 607
HpyCH4V TGCA 5 cut(s) 9, 76, 243, 500, 797
HpyF10VI GCNNNNNNNGC 2 cut(s) 557, 632
HpyF3I CTNAG 4 cut(s) 263, 399, 462, 804
HpySE526I ACGT 2 cut(s) 56, 607
Hsp92II CATG 4 cut(s) 436, 454, 754, 778
Kzo9I GATC 4 cut(s) 3, 182, 652, 807
LmnI GCTCC 1 cut(s) 412
Lsp1109I GCAGC 2 cut(s) 186, 382
LweI GCATC 3 cut(s) 424, 448, 487
MaeI CTAG 1 cut(s) 817
MaeII ACGT 2 cut(s) 56, 607
MaeIII GTNAC 4 cut(s) 14, 52, 377, 572
MalI GATC 4 cut(s) 5, 184, 654, 809
MboI GATC 4 cut(s) 3, 182, 652, 807
MboII GAAGA 1 cut(s) 694
MflI RGATCY 1 cut(s) 182
MlsI TGGCCA 1 cut(s) 749
MluCI AATT 6 cut(s) 96, 112, 336, 366, 446, 687
MluNI TGGCCA 1 cut(s) 749
MmeI TCCRAC 1 cut(s) 577
MnlI CCTC 6 cut(s) 138, 208, 230, 301, 643, 672
Mox20I TGGCCA 1 cut(s) 749
MscI TGGCCA 1 cut(s) 749
MseI TTAA 1 cut(s) 324
MslI CAYNNNNRTG 1 cut(s) 582
Msp20I TGGCCA 1 cut(s) 749
MspI CCGG 1 cut(s) 532
MspR9I CCNGG 2 cut(s) 43, 533
MvaI CCWGG 1 cut(s) 43
MwoI GCNNNNNNNGC 2 cut(s) 557, 632
NciI CCSGG 1 cut(s) 533
NdeII GATC 4 cut(s) 3, 182, 652, 807
NlaIII CATG 4 cut(s) 436, 454, 754, 778
NlaIV GGNNCC 3 cut(s) 122, 184, 770
NmuCI GTSAC 1 cut(s) 377
NspI RCATGY 1 cut(s) 778
PagI TCATGA 2 cut(s) 432, 450
PfeI GAWTC 4 cut(s) 427, 479, 512, 602
PflMI CCANNNNNTGG 1 cut(s) 117
PkrI GCNGC 2 cut(s) 201, 397
Ppu21I YACGTR 1 cut(s) 608
Psp6I CCWGG 1 cut(s) 41
PspEI GGTNACC 1 cut(s) 572
PspFI CCCAGC 1 cut(s) 698
PspGI CCWGG 1 cut(s) 41
PspN4I GGNNCC 3 cut(s) 122, 184, 770
PstNI CAGNNNCTG 1 cut(s) 803
PsuI RGATCY 1 cut(s) 182
RsaI GTAC 1 cut(s) 610
RsaNI GTAC 1 cut(s) 609
RseI CAYNNNNRTG 1 cut(s) 582
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 2 cut(s) 200, 396
Sau3AI GATC 4 cut(s) 3, 182, 652, 807
ScrFI CCNGG 2 cut(s) 43, 533
SetI ASST 9 cut(s) 59, 137, 166, 256, 472, 610, 637, 805, 818
SfaNI GCATC 3 cut(s) 424, 448, 487
SmiMI CAYNNNNRTG 1 cut(s) 582
Sse9I AATT 6 cut(s) 96, 112, 336, 366, 446, 687
SsiI CCGC 4 cut(s) 270, 384, 738, 772
SspMI CTAG 1 cut(s) 817
StyD4I CCNGG 2 cut(s) 41, 531
TaaI ACNGT 2 cut(s) 352, 613
TaiI ACGT 2 cut(s) 59, 610
TaqI TCGA 5 cut(s) 137, 278, 372, 667, 810
TaqII GACCGA 1 cut(s) 509
TasI AATT 6 cut(s) 96, 112, 336, 366, 446, 687
TfiI GAWTC 4 cut(s) 427, 479, 512, 602
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 1 cut(s) 377
TseI GCWGC 2 cut(s) 199, 395
Tsp45I GTSAC 1 cut(s) 377
TspDTI ATGAA 3 cut(s) 189, 439, 449
TspGWI ACGGA 2 cut(s) 175, 185
TspRI CASTG 1 cut(s) 238
Van91I CCANNNNNTGG 1 cut(s) 117
XapI RAATTY 2 cut(s) 96, 366
XceI RCATGY 1 cut(s) 778
XmiI GTMKAC 1 cut(s) 59
XspI CTAG 1 cut(s) 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.