pycom08g07690
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0009047
Physical Location & Seq
Forward (+)
6195355 .. 6196845
1491 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 978 bp
ATGGGCGTGGAACTAATGATGGGATTGGTTGACAGTTTTGCAGCGAGGATGGAGGAGAATGCGGTGAAGGAGGCGGCCTCCGCCGGAATCCAGAGCGTCGAGAAGTTCATTAGCCTGATTTCGGAACACCACCAATCAACGTCGTCGAACTCGGAAGCTGGTACGGAGTATAAAGCCGTGGCGGACATGGCCGTGACCAAGTTCAGAAAGGTCATTTCGTTGTTGGACAAGGGATGTACCGGCCACGCCCGGTTTCGGAAAGCTCCGGTGCCTCTATCTCCGCCTCCCCTGCAGCTGGTGCCTCCGAAGACGATAACCCAAATTCTGAGACCTTCAATTTCAGAACCCCATGATCAAACAACAGAGCAACCCTCTGTTTTCAGAACAGAACAATCTTCTGCCTTTAAGGTTTATTGTCCGAAGCCGGTGGTTCGCCTGCCGCCGCTGCCGCAAAATCCCCATTTGAAAACCAGTCCGGTTGTGCTGACAAAAGATATTGGGTGCTCTGAGAGAAAGATGGATTCTGCTGCTGCCGCCACCATCAACTTCTCGCCTTTGGCGCCCATTTCTGCCGCGAATTCGTTCATTTCGTCTTTAACTACTGGGGATCCCGCTGAGCAATCGATCTCTTCTGGGTTTCAATTTACAAATATGTCACAGTCGTCTTCGGGTAGGCCGCCTCTGTCTTCGTCGTCGTTGAAGAGGAAGTGCAGCTCCGTCGACAATGTTTCTCGTCTCCGGTGCGGATCTACTACCGGGCGCTGCCACTGCTCTAAGAAAAGGGTTCCTGCAATTAGCATGAAACTGGCTGATATTCCACCTGATGATTATTCTTGGAGAAAGTACGGTCAAAAACCCATCAAGGGTTCCCCTCATCCAAGAGGGTATTACAAGTGTAGCAGCCAGAGAGGTTGCCTTGCACGCAAGCACGTGGAGCGCGCTGTAGACGATCCGACCATGCTAGGCCGGAGATCGTGA

Protein Analysis

326

Amino Acids

35.3

Weight (kDa)

9.63

Isoelectric Point (pI)

57.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 275 - 320 9.3e-20 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 268, 298, 559
AccI GTMKAC 2 cut(s) 720, 945
AccII CGCG 2 cut(s) 575, 939
AclWI GGATC 4 cut(s) 602, 615, 754, 944
AcoI YGGCCR 2 cut(s) 189, 241
AcsI RAATTY 2 cut(s) 321, 577
AcvI CACGTG 1 cut(s) 931
AcyI GRCGYC 1 cut(s) 560
AfaI GTAC 3 cut(s) 163, 238, 845
AfiI CCNNNNNNNGG 4 cut(s) 121, 255, 295, 863
AgsI TTSAA 4 cut(s) 336, 466, 641, 700
AluBI AGCT 4 cut(s) 158, 263, 295, 714
AluI AGCT 4 cut(s) 158, 263, 295, 714
Alw21I GWGCWC 1 cut(s) 506
Alw26I GTCTC 2 cut(s) 322, 740
AlwI GGATC 4 cut(s) 602, 615, 754, 944
AoxI GGCC 5 cut(s) 75, 189, 241, 674, 964
ApeKI GCWGC 8 cut(s) 41, 292, 445, 527, 530, 711, 762, 900
ApoI RAATTY 2 cut(s) 321, 577
Asp700I GAANNNNTTC 1 cut(s) 581
AspLEI GCGC 4 cut(s) 562, 762, 939, 941
AsuC2I CCSGG 2 cut(s) 250, 757
AsuHPI GGTGA 1 cut(s) 76
BamHI GGATCC 1 cut(s) 607
BanI GGYRCC 3 cut(s) 268, 298, 559
BbrPI CACGTG 1 cut(s) 931
BbsI GAAGAC 3 cut(s) 314, 657, 678
Bbv12I GWGCWC 1 cut(s) 506
BbvI GCAGC 8 cut(s) 53, 304, 432, 514, 517, 723, 749, 912
BccI CCATC 5 cut(s) 13, 43, 511, 548, 866
BceAI ACGGC 2 cut(s) 161, 176
BcgI CGANNNNNNTGC 2 cut(s) 700, 734
BclI TGATCA 1 cut(s) 352
BcnI CCSGG 2 cut(s) 250, 757
BcoDI GTCTC 2 cut(s) 322, 740
BfaI CTAG 1 cut(s) 962
BfmI CTRYAG 2 cut(s) 290, 942
BfoI RGCGCY 2 cut(s) 563, 763
BlpI GCTNAGC 1 cut(s) 615
Bme1390I CCNGG 2 cut(s) 250, 757
BmiI GGNNCC 6 cut(s) 270, 300, 561, 609, 786, 868
BmrFI CCNGG 2 cut(s) 250, 757
BmrI ACTGGG 1 cut(s) 612
BmuI ACTGGG 1 cut(s) 612
BpiI GAAGAC 3 cut(s) 314, 657, 678
BplI GAGNNNNNCTC 4 cut(s) 62, 94, 356, 388
Bpu1102I GCTNAGC 1 cut(s) 615
BpuMI CCSGG 2 cut(s) 250, 757
Bsa29I ATCGAT 1 cut(s) 623
BsaAI YACGTR 1 cut(s) 931
BsaHI GRCGYC 1 cut(s) 560
BsaI GGTCTC 1 cut(s) 322
BsaJI CCNNGG 1 cut(s) 177
BsaWI WCCGGW 3 cut(s) 265, 475, 738
Bsc4I CCNNNNNNNGG 4 cut(s) 121, 255, 295, 863
Bse118I RCCGGY 2 cut(s) 239, 424
Bse1I ACTGG 3 cut(s) 471, 607, 810
BseCI ATCGAT 1 cut(s) 623
BseDI CCNNGG 1 cut(s) 177
BseGI GGATG 3 cut(s) 54, 239, 874
BseLI CCNNNNNNNGG 4 cut(s) 121, 255, 295, 863
BseMII CTCAG 3 cut(s) 317, 498, 606
BseNI ACTGG 3 cut(s) 471, 607, 810
BsePI GCGCGC 1 cut(s) 937
BseRI GAGGAG 1 cut(s) 68
BseXI GCAGC 8 cut(s) 53, 304, 432, 514, 517, 723, 749, 912
BsgI GTGCAG 1 cut(s) 730
Bsh1236I CGCG 2 cut(s) 575, 939
BshFI GGCC 5 cut(s) 77, 191, 243, 676, 966
BshNI GGYRCC 3 cut(s) 268, 298, 559
BshVI ATCGAT 1 cut(s) 623
BsiHKAI GWGCWC 1 cut(s) 506
BsiSI CCGG 9 cut(s) 84, 240, 250, 266, 425, 476, 739, 756, 967
BslI CCNNNNNNNGG 4 cut(s) 121, 255, 295, 863
BsmAI GTCTC 2 cut(s) 322, 740
BsmBI CGTCTC 1 cut(s) 740
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 5 cut(s) 77, 191, 243, 676, 966
Bso31I GGTCTC 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 506
Bsp143I GATC 6 cut(s) 352, 607, 624, 746, 949, 971
Bsp1720I GCTNAGC 1 cut(s) 615
BspANI GGCC 5 cut(s) 77, 191, 243, 676, 966
BspCNI CTCAG 3 cut(s) 318, 499, 607
BspDI ATCGAT 1 cut(s) 623
BspFNI CGCG 2 cut(s) 575, 939
BspLI GGNNCC 6 cut(s) 270, 300, 561, 609, 786, 868
BspMAI CTGCAG 1 cut(s) 294
BspPI GGATC 4 cut(s) 602, 615, 754, 944
BspT107I GGYRCC 3 cut(s) 268, 298, 559
BspTNI GGTCTC 1 cut(s) 322
BsrFI RCCGGY 2 cut(s) 239, 424
BsrI ACTGG 3 cut(s) 471, 607, 810
BssAI RCCGGY 2 cut(s) 239, 424
BssECI CCNNGG 1 cut(s) 177
BssHII GCGCGC 1 cut(s) 937
BssMI GATC 6 cut(s) 352, 607, 624, 746, 949, 971
BssNI GRCGYC 1 cut(s) 560
Bst4CI ACNGT 3 cut(s) 35, 660, 848
Bst6I CTCTTC 2 cut(s) 634, 695
BstACI GRCGYC 1 cut(s) 560
BstAPI GCANNNNNTGC 1 cut(s) 298
BstBAI YACGTR 1 cut(s) 931
BstC8I GCNNGC 4 cut(s) 437, 922, 926, 939
BstDEI CTNAG 4 cut(s) 326, 507, 615, 774
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 3 cut(s) 54, 239, 874
BstFNI CGCG 2 cut(s) 575, 939
BstH2I RGCGCY 2 cut(s) 563, 763
BstHHI GCGC 4 cut(s) 562, 762, 939, 941
BstKTI GATC 6 cut(s) 355, 610, 627, 749, 952, 974
BstMAI GTCTC 2 cut(s) 322, 740
BstMBI GATC 6 cut(s) 352, 607, 624, 746, 949, 971
BstSCI CCNGG 2 cut(s) 248, 755
BstSFI CTRYAG 2 cut(s) 290, 942
BstUI CGCG 2 cut(s) 575, 939
BstV1I GCAGC 8 cut(s) 53, 304, 432, 514, 517, 723, 749, 912
BstV2I GAAGAC 3 cut(s) 314, 657, 678
BstX2I RGATCY 2 cut(s) 607, 746
BstYI RGATCY 2 cut(s) 607, 746
Bsu15I ATCGAT 1 cut(s) 623
BsuRI GGCC 5 cut(s) 77, 191, 243, 676, 966
BsuTUI ATCGAT 1 cut(s) 623
BtgI CCRYGG 1 cut(s) 177
BtsCI GGATG 3 cut(s) 54, 239, 874
BtsI GCAGTG 1 cut(s) 766
BtsIMutI CAGTG 1 cut(s) 766
Cac8I GCNNGC 4 cut(s) 437, 922, 926, 939
CfoI GCGC 4 cut(s) 562, 762, 939, 941
Cfr10I RCCGGY 2 cut(s) 239, 424
ClaI ATCGAT 1 cut(s) 623
CseI GACGC 1 cut(s) 85
Csp6I GTAC 3 cut(s) 162, 237, 844
CviAII CATG 4 cut(s) 187, 350, 799, 958
CviQI GTAC 3 cut(s) 162, 237, 844
DdeI CTNAG 4 cut(s) 326, 507, 615, 774
DinI GGCGCC 1 cut(s) 561
DpnI GATC 6 cut(s) 354, 609, 626, 748, 951, 973
DpnII GATC 6 cut(s) 352, 607, 624, 746, 949, 971
EaeI YGGCCR 2 cut(s) 189, 241
Eam1104I CTCTTC 2 cut(s) 634, 695
EarI CTCTTC 2 cut(s) 634, 695
EciI GGCGGA 3 cut(s) 70, 197, 270
Eco31I GGTCTC 1 cut(s) 322
Eco72I CACGTG 1 cut(s) 931
EcoRI GAATTC 1 cut(s) 577
EgeI GGCGCC 1 cut(s) 561
EheI GGCGCC 1 cut(s) 561
Esp3I CGTCTC 1 cut(s) 740
FaeI CATG 4 cut(s) 190, 353, 802, 961
FaiI YATR 6 cut(s) 171, 188, 351, 653, 800, 959
FatI CATG 4 cut(s) 186, 349, 798, 957
FauI CCCGC 1 cut(s) 619
FbaI TGATCA 1 cut(s) 352
FblI GTMKAC 2 cut(s) 720, 945
FokI GGATG 3 cut(s) 61, 246, 861
FspBI CTAG 1 cut(s) 962
GlaI GCGC 4 cut(s) 561, 761, 938, 940
HaeII RGCGCY 2 cut(s) 563, 763
HaeIII GGCC 5 cut(s) 77, 191, 243, 676, 966
HapII CCGG 9 cut(s) 84, 240, 250, 266, 425, 476, 739, 756, 967
HgaI GACGC 1 cut(s) 85
HhaI GCGC 4 cut(s) 562, 762, 939, 941
Hin1I GRCGYC 1 cut(s) 560
Hin1II CATG 4 cut(s) 190, 353, 802, 961
Hin6I GCGC 4 cut(s) 560, 760, 937, 939
HinP1I GCGC 4 cut(s) 560, 760, 937, 939
HincII GTYRAC 2 cut(s) 31, 721
HindII GTYRAC 2 cut(s) 31, 721
HinfI GANTC 2 cut(s) 87, 521
HpaII CCGG 9 cut(s) 84, 240, 250, 266, 425, 476, 739, 756, 967
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 3 cut(s) 31, 721, 946
Hpy188III TCNNGA 3 cut(s) 91, 100, 975
Hpy8I GTNNAC 3 cut(s) 31, 721, 946
Hpy99I CGWCG 6 cut(s) 101, 145, 148, 694, 697, 722
HpyAV CCTTC 2 cut(s) 61, 342
HpyCH4III ACNGT 3 cut(s) 35, 660, 848
HpyCH4IV ACGT 2 cut(s) 140, 930
HpyCH4V TGCA 5 cut(s) 41, 292, 711, 791, 920
HpyF3I CTNAG 4 cut(s) 326, 507, 615, 774
HpySE526I ACGT 2 cut(s) 140, 930
Hsp92I GRCGYC 1 cut(s) 560
Hsp92II CATG 4 cut(s) 190, 353, 802, 961
HspAI GCGC 4 cut(s) 560, 760, 937, 939
KasI GGCGCC 1 cut(s) 559
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 6 cut(s) 352, 607, 624, 746, 949, 971
LmnI GCTCC 3 cut(s) 268, 719, 934
Lsp1109I GCAGC 8 cut(s) 53, 304, 432, 514, 517, 723, 749, 912
MaeI CTAG 1 cut(s) 962
MaeII ACGT 2 cut(s) 140, 930
MaeIII GTNAC 2 cut(s) 193, 654
MalI GATC 6 cut(s) 354, 609, 626, 748, 951, 973
MboI GATC 6 cut(s) 352, 607, 624, 746, 949, 971
MboII GAAGA 6 cut(s) 319, 387, 621, 657, 678, 712
MflI RGATCY 2 cut(s) 607, 746
MhlI GDGCHC 1 cut(s) 506
MluCI AATT 5 cut(s) 321, 336, 577, 641, 792
Mly113I GGCGCC 1 cut(s) 560
MmeI TCCRAC 2 cut(s) 204, 977
MroXI GAANNNNTTC 1 cut(s) 581
MseI TTAA 2 cut(s) 405, 596
MslI CAYNNNNRTG 1 cut(s) 191
MspA1I CMGCKG 3 cut(s) 295, 445, 614
MspI CCGG 9 cut(s) 84, 240, 250, 266, 425, 476, 739, 756, 967
MspR9I CCNGG 2 cut(s) 250, 757
Mva1269I GAATGC 1 cut(s) 64
MvnI CGCG 2 cut(s) 575, 939
NarI GGCGCC 1 cut(s) 560
NciI CCSGG 2 cut(s) 250, 757
NdeII GATC 6 cut(s) 352, 607, 624, 746, 949, 971
NlaIII CATG 4 cut(s) 190, 353, 802, 961
NlaIV GGNNCC 6 cut(s) 270, 300, 561, 609, 786, 868
NmuCI GTSAC 2 cut(s) 193, 654
PauI GCGCGC 1 cut(s) 937
PcsI WCGNNNNNNNCGW 1 cut(s) 587
PctI GAATGC 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 581
PfeI GAWTC 2 cut(s) 87, 521
PluTI GGCGCC 1 cut(s) 563
PmaCI CACGTG 1 cut(s) 931
PmlI CACGTG 1 cut(s) 931
Ppu21I YACGTR 1 cut(s) 931
PspCI CACGTG 1 cut(s) 931
PspN4I GGNNCC 6 cut(s) 270, 300, 561, 609, 786, 868
PstI CTGCAG 1 cut(s) 294
PsuI RGATCY 2 cut(s) 607, 746
PteI GCGCGC 1 cut(s) 937
PvuII CAGCTG 1 cut(s) 295
RsaI GTAC 3 cut(s) 163, 238, 845
RsaNI GTAC 3 cut(s) 162, 237, 844
RseI CAYNNNNRTG 1 cut(s) 191
SalI GTCGAC 1 cut(s) 719
SaqAI TTAA 2 cut(s) 405, 596
Sau3AI GATC 6 cut(s) 352, 607, 624, 746, 949, 971
ScrFI CCNGG 2 cut(s) 250, 757
SduI GDGCHC 1 cut(s) 506
SfcI CTRYAG 2 cut(s) 290, 942
SfoI GGCGCC 1 cut(s) 561
SmiMI CAYNNNNRTG 1 cut(s) 191
Sse9I AATT 5 cut(s) 321, 336, 577, 641, 792
SspDI GGCGCC 1 cut(s) 559
SspMI CTAG 1 cut(s) 962
StyD4I CCNGG 2 cut(s) 248, 755
TaaI ACNGT 3 cut(s) 35, 660, 848
TaiI ACGT 2 cut(s) 143, 933
TaqI TCGA 4 cut(s) 99, 146, 623, 720
TasI AATT 5 cut(s) 321, 336, 577, 641, 792
TauI GCSGC 7 cut(s) 77, 442, 445, 451, 536, 575, 679
TfiI GAWTC 2 cut(s) 87, 521
Tru1I TTAA 2 cut(s) 405, 596
Tru9I TTAA 2 cut(s) 405, 596
TscAI CASTG 1 cut(s) 773
TseFI GTSAC 2 cut(s) 193, 654
TseI GCWGC 8 cut(s) 41, 292, 445, 527, 530, 711, 762, 900
Tsp45I GTSAC 2 cut(s) 193, 654
TspDTI ATGAA 3 cut(s) 97, 574, 815
TspGWI ACGGA 2 cut(s) 179, 706
TspRI CASTG 1 cut(s) 773
XapI RAATTY 2 cut(s) 321, 577
XmiI GTMKAC 2 cut(s) 720, 945
XmnI GAANNNNTTC 1 cut(s) 581
XspI CTAG 1 cut(s) 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.