pycom08g15080

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003440
Physical Location & Seq
Forward (+)
14584690 .. 14589460
4771 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3021 bp
ATGTCGAGGATCACTAGGTGGAAGCTTGAGAAGACAAAAGTAAAAGTGGTTTTCAGGTTACAGTTCCATGCCACACATGTTCCACAAACCGGATGGGATAAGCTGTTTATATCTTTCATACCTGCTGATTCCGGAAAAGCAACTGCAAAAACAACCAAGGCAAACGTGAGGAATGGGACCTGCAAATGGGGAGACCCCATCTATGAAACTACAAGGCTTCTACAAGACACCAAAACCAAACAATACGATGAGAAGCTTTACAAACTTGTGGTAACCATGGGTTCTTCAAGGTCTAGTATCCTTGGTGAGACTAATATCAACCTTGCTGATTATGCTGATGCATCAAAGCCTTCGTCCGTTGCTCTGCCTCTTCTTGGATGTGATTCTGGAACTGTTTTACATGTCACGGTACAGCTGCTAACTTCTAAAACTGGCTTCAGAGAGTTTGAGCAGCAGAGGGAGCTCAGAGAGAGCGGTCTACGAACAACCTCTGACCAAAACAGAAATGATGTATCCACTACTAGAAGAATATCATCTTCTGAAGATGCAGTAAATGAGCAGATGGATAAGATGAATTTGAAAGTTAGATTCAAAGAACTCTGTCCCCATGAAGAAGGGGCTGGTCTGAATGAAGAGTATGCTGATTCAACTGGTGGATTTGAATATTCTTCCACTACTTCAGAAAGTTTATATGCTGAGAAACATGACGCGTCTAGCACCCATGAGATTGACAGTATTAAGAGTACCACCTCTGCTGATCTAGGGGGATTGTCCCTTAGTCAAAGTCCCGGACAAGAGAAACAACATCCCTCGGATCAGCAATTCTTGGTACAGGGGACCAGTGAGTGGGCTCATAGCTGGGGCTCTGAATTTTCTCCAGATGCTTATTTACCAAATGCTTCTGAGGAAAATAGCAGGCTTAGCAGAAGCTTGGAAGCAGCTGAATCATCCATTCTTGACCTTAAGCAGGAGGTGAACTCTTTACAGGTTCATGCTGATGAAATCGGCAATGATGCAGAAAAATTTAGTCTTCAACTTGATGCTGAAATTGCTTCTGGAGAACAATTAGCAAAAGAAATTTCCACACTGAGATCCGAATGTTCAAAGTTAAAAGAAGATCTTGAGGAGCAAAAAAGTTTCAAACTAAGCATTCCATTCTCCCACAGGGAAACTATTGAGACAGGCCAAGATCGCATTTTTCATGAGTTACGGCTCAGGTGGTTTAAGGGCCTTTCAAGCATGGAGGATAAATTAAAAGGGCTTCAGAGCAAGGCATTATTTGGAGCAAATGAAAGGGACTTCCAGTCCTGTCACTCGGATTTCGAGGCATTGCTTGGTGTTTTGCAAGTTCTCAAACAAGAGACTGGACAGGCAAGTTCAGGGCTGAACGTGACAAGCGTAAAGGCTGATGAAATAAGTTTACATAAAGGTGAGCAATTAGTACTAGGAACGAGGGTGGATGCAGATTCCCATCAACCCGAGGGTGCCGTTCATTGTCTAAGTATACCTGGCCTGGTGTCACTAGACTTTGATTCTGTAGATGCTGCCAATGCAATGAAAGGGAAGTTTGTTGAAATTTTAAGAGAGTTGGATGAGATGAAAGCTGATCGAGAAAGCCTTGCAAAGAAAGCAGACCAGATGGAGTGCTACTATGAAGCTCTCATTCATGAACTTGAGGAAAACCAGAGACAGATGATTGGAGAATTGCAGAATCTCAGGAATGAGCATTCTACTTGCTTGTACACAATTTCATCTGCTAACGCTGAGATGGAGAGAATCCAGCAAGACATGAACAACGAGCGAAGAATATTTTCCAAAGAAAAGCGTGATTTGGACTCTCTGAACAAGGAGTTTGAAAGAAGGGCTACTACAGCAGAAGCAGCACTTAAAAGGGCACGCATGAATTACTCTATTGCAGTGAACCAGTTACAGAAGGACCTTGAATTGCTTTCATTCCAGGTTCAGTCCATGCATGAGACTAATGAGAACCTCATTAAGCAGGCTTTTGAAGATTCTTTGATTCCAATGTTTCAGGGTTGTGAAGAAACAGTGCAGAATAAGAAATCGGATTCAGAGGAATTTCCATCTGCAAAACACTTACAGTGTCAGAATCAATGCTACGGGATAAAGAAACAGCAGTTAGATGGGAATGCATTATCAGATGATTTGAGAAGATCCCTCCACTTGCAGAAGGGTATCTACCAAAAGATTGAAGAAGAACTTTATGAGGTGCATCTGGTGAATGTGTATTTGGATGTATTGTCAAAGACTCTACAAGTTACTTTGATTGAAGCAAGTGCTGCCTTCGGATTGACGAAAGAGAAAGTGCATGAACTTTCACAACAGCTGGAGCTTTCAACTGAGTCCAATGAGTTGTTGATGCTGAGGCTGCAGACTGCATTGGATGAGATTCGCCGCCTGAAGGAGTACAAGGAAACTTGTAATTCAATCCGCAATGACTTGGTTCTGAAGAACCAAATTTTGGAAGCAGATTTACAAAACGCTATTAGTGAAAATGGCCTTCTTACCCAGAAGATTGTTGAATGGAAAGGTATGATAAAAGAATATGAAACTTATGAGAGCAAATACAAGGCCTGCACTACTGAAAAATTACAGCTGGAAAATTTGTTGCAAAAGGAAACCCTAGAAAACGGCATTCTTCAGAATAAACTTTCCTCTTTGCAGGAAGAGTTGAAATCTGTCAGAACCGACTTTGATGAGCTAGCTTGCACGAAGGAGGATCTGCAGAATATTGTGAATTTTCTACAGGGTAAGTTATGGAATCTGTTGGCATCATATGACCAAAAGTATAAAAGCCTGGCTCTTTGCGGCGGATGTGTCTGTCAAGGCTTGGAATCCAGAGATTTAACAGGTGTAGTGATGCAAATAGAAGAGCTTCAGAACAATGTATATGGAAAGTTTCTCCAAATGATGGAAGAGAAGAAAGAGCTAGCACAAGAAAGAGATATTGCTCAAGAGTCTTTACGTGCAGCAGAGTCTGATAATCTGATCGATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1007

Amino Acids

113.93

Weight (kDa)

5.1

Isoelectric Point (pI)

50.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 11 - 141 1.3e-26 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 130, 188
AccB1I GGYRCC 1 cut(s) 1484
AccB7I CCANNNNNTGG 3 cut(s) 846, 2482, 2932
AccBSI CCGCTC 1 cut(s) 474
AccI GTMKAC 2 cut(s) 478, 1504
AccII CGCG 1 cut(s) 710
AccIII TCCGGA 1 cut(s) 131
AciI CCGC 5 cut(s) 474, 2416, 2452, 2829, 2832
AclWI GGATC 5 cut(s) 17, 822, 1086, 2169, 2748
AcsI RAATTY 9 cut(s) 574, 869, 1022, 1077, 1575, 2078, 2478, 2623, 2758
AcuI CTGAAG 8 cut(s) 421, 561, 663, 1247, 2441, 2489, 2645, 2882
AdeI CACNNNGTG 1 cut(s) 18
AfaI GTAC 6 cut(s) 411, 745, 831, 1443, 1742, 2429
AfiI CCNNNNNNNGG 8 cut(s) 89, 186, 374, 846, 967, 2422, 2482, 2932
AflII CTTAAG 1 cut(s) 962
AflIII ACRYGT 3 cut(s) 76, 400, 708
AhdI GACNNNNNGTC 1 cut(s) 1303
AjnI CCWGG 4 cut(s) 1507, 1512, 1956, 2817
AjuI GAANNNNNNNTTGG 4 cut(s) 2234, 2266, 2465, 2497
Alw21I GWGCWC 1 cut(s) 465
Alw26I GTCTC 6 cut(s) 186, 302, 1172, 1355, 1681, 1970
AlwI GGATC 5 cut(s) 17, 822, 1086, 2169, 2748
AlwNI CAGNNNCTG 1 cut(s) 2999
Ama87I CYCGRG 1 cut(s) 1478
Aor13HI TCCGGA 1 cut(s) 131
AoxI GGCC 5 cut(s) 1183, 1228, 1510, 2518, 2592
ApeKI GCWGC 8 cut(s) 415, 451, 938, 1544, 1880, 2300, 2389, 2990
ApoI RAATTY 9 cut(s) 574, 869, 1022, 1077, 1575, 2078, 2478, 2623, 2758
ArsI GACNNNNNNTTYG 2 cut(s) 338, 370
Asp700I GAANNNNTTC 2 cut(s) 1810, 2895
AspS9I GGNCC 4 cut(s) 177, 837, 1228, 1936
AsuC2I CCSGG 1 cut(s) 789
AsuHPI GGTGA 4 cut(s) 317, 985, 1442, 2251
AsuNHI GCTAGC 2 cut(s) 2722, 2950
AvaI CYCGRG 1 cut(s) 1478
AvaII GGWCC 3 cut(s) 177, 837, 1936
BaeGI GKGCMC 1 cut(s) 1897
BanI GGYRCC 1 cut(s) 1484
BanII GRGCYC 3 cut(s) 465, 853, 866
BarI GAAGNNNNNNTAC 6 cut(s) 1852, 1884, 2183, 2215, 2478, 2510
BbsI GAAGAC 2 cut(s) 38, 1022
Bbv12I GWGCWC 1 cut(s) 465
BbvCI CCTCAGC 1 cut(s) 2384
BbvI GCAGC 8 cut(s) 402, 463, 950, 1531, 1892, 2287, 2376, 3002
BccI CCATC 9 cut(s) 87, 206, 556, 1479, 1633, 1762, 2092, 2138, 2926
BceAI ACGGC 3 cut(s) 1226, 1472, 2668
BciT130I CCWGG 4 cut(s) 1509, 1514, 1958, 2819
BciVI GTATCC 2 cut(s) 308, 523
BcnI CCSGG 1 cut(s) 789
BcoDI GTCTC 6 cut(s) 186, 302, 1172, 1355, 1681, 1970
BfmI CTRYAG 5 cut(s) 1536, 1869, 2390, 2744, 2765
BfrI CTTAAG 1 cut(s) 962
BfuAI ACCTGC 2 cut(s) 130, 188
BfuI GTATCC 2 cut(s) 308, 523
BglII AGATCT 1 cut(s) 1117
BlpI GCTNAGC 1 cut(s) 920
BmcAI AGTACT 1 cut(s) 1443
Bme1390I CCNGG 5 cut(s) 789, 1509, 1514, 1958, 2819
Bme18I GGWCC 3 cut(s) 177, 837, 1936
BmeRI GACNNNNNGTC 1 cut(s) 1303
BmeT110I CYCGRG 1 cut(s) 1478
BmgT120I GGNCC 4 cut(s) 177, 837, 1228, 1936
BmiI GGNNCC 3 cut(s) 178, 838, 1486
BmrFI CCNGG 5 cut(s) 789, 1509, 1514, 1958, 2819
BmtI GCTAGC 2 cut(s) 2726, 2954
BpiI GAAGAC 2 cut(s) 38, 1022
BplI GAGNNNNNCTC 2 cut(s) 962, 994
BpmI CTGGAG 3 cut(s) 861, 1077, 2369
Bpu10I CCTNAGC 2 cut(s) 1214, 2384
Bpu1102I GCTNAGC 1 cut(s) 920
BpuEI CTTGAG 4 cut(s) 47, 1142, 1694, 2958
BpuMI CCSGG 1 cut(s) 789
Bsa29I ATCGAT 2 cut(s) 3012, 3016
BsaAI YACGTR 1 cut(s) 2987
BsaBI GATNNNNATC 1 cut(s) 1470
BsaI GGTCTC 1 cut(s) 186
BsaJI CCNNGG 5 cut(s) 156, 276, 301, 810, 1479
BsaWI WCCGGW 2 cut(s) 89, 131
Bsc4I CCNNNNNNNGG 8 cut(s) 89, 186, 374, 846, 967, 2422, 2482, 2932
Bse1I ACTGG 6 cut(s) 436, 655, 840, 1303, 1369, 1924
Bse3DI GCAATG 4 cut(s) 1015, 1328, 1560, 2461
Bse8I GATNNNNATC 1 cut(s) 1470
BseAI TCCGGA 1 cut(s) 131
BseBI CCWGG 4 cut(s) 1509, 1514, 1958, 2819
BseCI ATCGAT 2 cut(s) 3012, 3016
BseDI CCNNGG 5 cut(s) 156, 276, 301, 810, 1479
BseGI GGATG 9 cut(s) 98, 383, 805, 947, 1465, 1597, 2260, 2410, 2840
BseJI GATNNNNATC 1 cut(s) 1470
BseLI CCNNNNNNNGG 8 cut(s) 89, 186, 374, 846, 967, 2422, 2482, 2932
BseMI GCAATG 4 cut(s) 1015, 1328, 1560, 2461
BseMII CTCAG 9 cut(s) 478, 687, 894, 1079, 1228, 1729, 1755, 2352, 2375
BseNI ACTGG 6 cut(s) 436, 655, 840, 1303, 1369, 1924
BseRI GAGGAG 1 cut(s) 1139
BseSI GKGCMC 1 cut(s) 1897
BseXI GCAGC 8 cut(s) 402, 463, 950, 1531, 1892, 2287, 2376, 3002
BseYI CCCAGC 1 cut(s) 858
BsgI GTGCAG 3 cut(s) 2072, 2581, 3009
Bsh1236I CGCG 1 cut(s) 710
Bsh1285I CGRYCG 1 cut(s) 3016
BshFI GGCC 5 cut(s) 1185, 1230, 1512, 2520, 2594
BshNI GGYRCC 1 cut(s) 1484
BshVI ATCGAT 2 cut(s) 3012, 3016
BsiEI CGRYCG 1 cut(s) 3016
BsiHKAI GWGCWC 1 cut(s) 465
BsiHKCI CYCGRG 1 cut(s) 1478
BsiSI CCGG 3 cut(s) 90, 132, 789
BslFI GGGAC 6 cut(s) 190, 588, 757, 771, 850, 1310
BslI CCNNNNNNNGG 8 cut(s) 89, 186, 374, 846, 967, 2422, 2482, 2932
BsmAI GTCTC 6 cut(s) 186, 302, 1172, 1355, 1681, 1970
BsmFI GGGAC 6 cut(s) 190, 588, 757, 771, 850, 1310
BsmI GAATGC 4 cut(s) 1149, 1726, 2155, 2655
BsnI GGCC 5 cut(s) 1185, 1230, 1512, 2520, 2594
Bso31I GGTCTC 1 cut(s) 186
BsoBI CYCGRG 1 cut(s) 1478
Bsp1286I GDGCHC 4 cut(s) 465, 853, 866, 1897
Bsp13I TCCGGA 1 cut(s) 131
Bsp1407I TGTACA 1 cut(s) 1740
Bsp1720I GCTNAGC 1 cut(s) 920
Bsp19I CCATGG 1 cut(s) 276
BspACI CCGC 5 cut(s) 474, 2416, 2452, 2829, 2832
BspANI GGCC 5 cut(s) 1185, 1230, 1512, 2520, 2594
BspCNI CTCAG 9 cut(s) 477, 688, 895, 1080, 1227, 1728, 1756, 2353, 2376
BspDI ATCGAT 2 cut(s) 3012, 3016
BspEI TCCGGA 1 cut(s) 131
BspFNI CGCG 1 cut(s) 710
BspHI TCATGA 2 cut(s) 1201, 1666
BspLI GGNNCC 3 cut(s) 178, 838, 1486
BspMAI CTGCAG 2 cut(s) 2394, 2748
BspMI ACCTGC 2 cut(s) 130, 188
BspOI GCTAGC 2 cut(s) 2726, 2954
BspPI GGATC 5 cut(s) 17, 822, 1086, 2169, 2748
BspQI GCTCTTC 1 cut(s) 2886
BspT107I GGYRCC 1 cut(s) 1484
BspTI CTTAAG 1 cut(s) 962
BspTNI GGTCTC 1 cut(s) 186
BsrBI CCGCTC 1 cut(s) 474
BsrDI GCAATG 4 cut(s) 1015, 1328, 1560, 2461
BsrGI TGTACA 1 cut(s) 1740
BsrI ACTGG 6 cut(s) 436, 655, 840, 1303, 1369, 1924
BssECI CCNNGG 5 cut(s) 156, 276, 301, 810, 1479
BssNAI GTATAC 1 cut(s) 1505
BssT1I CCWWGG 3 cut(s) 156, 276, 301
Bst1107I GTATAC 1 cut(s) 1505
Bst2UI CCWGG 4 cut(s) 1509, 1514, 1958, 2819
Bst4CI ACNGT 6 cut(s) 63, 394, 409, 734, 2050, 2103
Bst6I CTCTTC 5 cut(s) 375, 627, 2682, 2886, 2931
BstAFI CTTAAG 1 cut(s) 962
BstAPI GCANNNNNTGC 1 cut(s) 2300
BstAUI TGTACA 1 cut(s) 1740
BstBAI YACGTR 1 cut(s) 2987
BstC8I GCNNGC 7 cut(s) 917, 1897, 2001, 2596, 2724, 2728, 2952
BstDSI CCRYGG 1 cut(s) 276
BstEII GGTNACC 1 cut(s) 271
BstENI CCTNNNNNAGG 1 cut(s) 965
BstF5I GGATG 9 cut(s) 98, 383, 805, 947, 1465, 1597, 2260, 2410, 2840
BstFNI CGCG 1 cut(s) 710
BstMAI GTCTC 6 cut(s) 186, 302, 1172, 1355, 1681, 1970
BstMCI CGRYCG 1 cut(s) 3016
BstNI CCWGG 4 cut(s) 1509, 1514, 1958, 2819
BstNSI RCATGY 2 cut(s) 80, 404
BstPI GGTNACC 1 cut(s) 271
BstSCI CCNGG 5 cut(s) 787, 1507, 1512, 1956, 2817
BstSFI CTRYAG 5 cut(s) 1536, 1869, 2390, 2744, 2765
BstSLI GKGCMC 1 cut(s) 1897
BstUI CGCG 1 cut(s) 710
BstV1I GCAGC 8 cut(s) 402, 463, 950, 1531, 1892, 2287, 2376, 3002
BstV2I GAAGAC 2 cut(s) 38, 1022
BstX2I RGATCY 4 cut(s) 1091, 1117, 2174, 2740
BstYI RGATCY 4 cut(s) 1091, 1117, 2174, 2740
BstZ17I GTATAC 1 cut(s) 1505
Bsu15I ATCGAT 2 cut(s) 3012, 3016
BsuI GTATCC 2 cut(s) 308, 523
BsuRI GGCC 5 cut(s) 1185, 1230, 1512, 2520, 2594
BsuTUI ATCGAT 2 cut(s) 3012, 3016
BtgI CCRYGG 1 cut(s) 276
BtsCI GGATG 9 cut(s) 98, 383, 805, 947, 1465, 1597, 2260, 2410, 2840
BtsI GCAGTG 1 cut(s) 1923
BtsIMutI CAGTG 5 cut(s) 847, 1085, 1923, 2055, 2108
BveI ACCTGC 2 cut(s) 130, 188
Cac8I GCNNGC 7 cut(s) 917, 1897, 2001, 2596, 2724, 2728, 2952
CaiI CAGNNNCTG 1 cut(s) 2999
CciI TCATGA 2 cut(s) 1201, 1666
Cfr13I GGNCC 4 cut(s) 177, 837, 1228, 1936
ClaI ATCGAT 2 cut(s) 3012, 3016
CseI GACGC 2 cut(s) 699, 716
Csp6I GTAC 6 cut(s) 410, 744, 830, 1442, 1741, 2428
CviQI GTAC 6 cut(s) 410, 744, 830, 1442, 1741, 2428
DraIII CACNNNGTG 1 cut(s) 18
DriI GACNNNNNGTC 1 cut(s) 1303
Eam1104I CTCTTC 5 cut(s) 375, 627, 2682, 2886, 2931
Eam1105I GACNNNNNGTC 1 cut(s) 1303
EarI CTCTTC 5 cut(s) 375, 627, 2682, 2886, 2931
EciI GGCGGA 1 cut(s) 2847
Ecl136II GAGCTC 1 cut(s) 463
Eco130I CCWWGG 3 cut(s) 156, 276, 301
Eco147I AGGCCT 1 cut(s) 2594
Eco24I GRGCYC 3 cut(s) 465, 853, 866
Eco31I GGTCTC 1 cut(s) 186
Eco47I GGWCC 3 cut(s) 177, 837, 1936
Eco53kI GAGCTC 1 cut(s) 463
Eco57I CTGAAG 8 cut(s) 421, 561, 663, 1247, 2441, 2489, 2645, 2882
Eco88I CYCGRG 1 cut(s) 1478
Eco91I GGTNACC 1 cut(s) 271
EcoICRI GAGCTC 1 cut(s) 463
EcoNI CCTNNNNNAGG 1 cut(s) 965
EcoO109I RGGNCCY 3 cut(s) 177, 1228, 1936
EcoO65I GGTNACC 1 cut(s) 271
EcoRII CCWGG 4 cut(s) 1507, 1512, 1956, 2817
EcoT14I CCWWGG 3 cut(s) 156, 276, 301
EcoT22I ATGCAT 3 cut(s) 343, 1974, 2155
EcoT38I GRGCYC 3 cut(s) 465, 853, 866
ErhI CCWWGG 3 cut(s) 156, 276, 301
FalI AAGNNNNNCTT 6 cut(s) 1104, 1136, 1869, 1901, 2205, 2237
FaqI GGGAC 6 cut(s) 190, 588, 757, 771, 850, 1310
FauNDI CATATG 1 cut(s) 2797
FblI GTMKAC 2 cut(s) 478, 1504
FokI GGATG 9 cut(s) 105, 390, 792, 934, 1472, 1604, 2267, 2417, 2847
FriOI GRGCYC 3 cut(s) 465, 853, 866
GsaI CCCAGC 1 cut(s) 862
GsuI CTGGAG 3 cut(s) 861, 1077, 2369
HaeIII GGCC 5 cut(s) 1185, 1230, 1512, 2520, 2594
HapII CCGG 3 cut(s) 90, 132, 789
HgaI GACGC 2 cut(s) 699, 716
HindIII AAGCTT 3 cut(s) 23, 254, 928
HpaII CCGG 3 cut(s) 90, 132, 789
HphI GGTGA 4 cut(s) 317, 985, 1442, 2251
Hpy166II GTNNAC 6 cut(s) 479, 976, 1421, 1505, 1743, 1921
Hpy8I GTNNAC 6 cut(s) 479, 976, 1421, 1505, 1743, 1921
HpyAV CCTTC 9 cut(s) 360, 608, 1854, 1927, 2185, 2314, 2416, 2531, 2728
HpyCH4III ACNGT 6 cut(s) 63, 394, 409, 734, 2050, 2103
HpyCH4IV ACGT 3 cut(s) 165, 1389, 2986
HpySE526I ACGT 3 cut(s) 165, 1389, 2986
Kpn2I TCCGGA 1 cut(s) 131
LguI GCTCTTC 1 cut(s) 2886
LmnI GCTCC 4 cut(s) 460, 1126, 1283, 2350
Lsp1109I GCAGC 8 cut(s) 402, 463, 950, 1531, 1892, 2287, 2376, 3002
MaeII ACGT 3 cut(s) 165, 1389, 2986
MaeIII GTNAC 9 cut(s) 57, 271, 403, 1206, 1310, 1390, 1518, 1926, 2278
MbiI CCGCTC 1 cut(s) 474
MflI RGATCY 4 cut(s) 1091, 1117, 2174, 2740
MhlI GDGCHC 4 cut(s) 465, 853, 866, 1897
MluI ACGCGT 1 cut(s) 708
MlyI GAGTC 5 cut(s) 1829, 2263, 2372, 2987, 3005
MmeI TCCRAC 1 cut(s) 1569
Mph1103I ATGCAT 3 cut(s) 343, 1974, 2155
MroI TCCGGA 1 cut(s) 131
MroXI GAANNNNTTC 2 cut(s) 1810, 2895
MseI TTAA 9 cut(s) 738, 963, 1109, 1224, 1253, 1580, 1887, 1995, 2867
MslI CAYNNNNRTG 2 cut(s) 996, 1428
MspA1I CMGCKG 4 cut(s) 415, 941, 2347, 2617
MspCI CTTAAG 1 cut(s) 962
MspI CCGG 3 cut(s) 90, 132, 789
MspR9I CCNGG 5 cut(s) 789, 1509, 1514, 1958, 2819
Mva1269I GAATGC 4 cut(s) 1149, 1726, 2155, 2655
MvaI CCWGG 4 cut(s) 1509, 1514, 1958, 2819
MvnI CGCG 1 cut(s) 710
NciI CCSGG 1 cut(s) 789
NcoI CCATGG 1 cut(s) 276
NdeI CATATG 1 cut(s) 2797
NheI GCTAGC 2 cut(s) 2722, 2950
NlaIV GGNNCC 3 cut(s) 178, 838, 1486
NmuCI GTSAC 4 cut(s) 403, 1310, 1390, 1518
NsiI ATGCAT 3 cut(s) 343, 1974, 2155
NspI RCATGY 2 cut(s) 80, 404
PagI TCATGA 2 cut(s) 1201, 1666
PceI AGGCCT 1 cut(s) 2594
PciI ACATGT 2 cut(s) 76, 400
PciSI GCTCTTC 1 cut(s) 2886
PcsI WCGNNNNNNNCGW 1 cut(s) 1395
PctI GAATGC 4 cut(s) 1149, 1726, 2155, 2655
PdmI GAANNNNTTC 2 cut(s) 1810, 2895
PflMI CCANNNNNTGG 3 cut(s) 846, 2482, 2932
PfoI TCCNGGA 1 cut(s) 787
Ple19I CGATCG 1 cut(s) 3016
PleI GAGTC 5 cut(s) 1829, 2263, 2371, 2986, 3004
PpsI GAGTC 5 cut(s) 1829, 2263, 2371, 2986, 3004
Ppu21I YACGTR 1 cut(s) 2987
PpuMI RGGWCCY 2 cut(s) 177, 1936
PscI ACATGT 2 cut(s) 76, 400
Psp124BI GAGCTC 1 cut(s) 465
Psp5II RGGWCCY 2 cut(s) 177, 1936
Psp6I CCWGG 4 cut(s) 1507, 1512, 1956, 2817
PspEI GGTNACC 1 cut(s) 271
PspFI CCCAGC 1 cut(s) 858
PspGI CCWGG 4 cut(s) 1507, 1512, 1956, 2817
PspN4I GGNNCC 3 cut(s) 178, 838, 1486
PspPI GGNCC 4 cut(s) 177, 837, 1228, 1936
PspPPI RGGWCCY 2 cut(s) 177, 1936
PstI CTGCAG 2 cut(s) 2394, 2748
PstNI CAGNNNCTG 1 cut(s) 2999
PsuI RGATCY 4 cut(s) 1091, 1117, 2174, 2740
PvuI CGATCG 1 cut(s) 3016
PvuII CAGCTG 4 cut(s) 415, 941, 2347, 2617
RsaI GTAC 6 cut(s) 411, 745, 831, 1443, 1742, 2429
RsaNI GTAC 6 cut(s) 410, 744, 830, 1442, 1741, 2428
RseI CAYNNNNRTG 2 cut(s) 996, 1428
SacI GAGCTC 1 cut(s) 465
SapI GCTCTTC 1 cut(s) 2886
SaqAI TTAA 9 cut(s) 738, 963, 1109, 1224, 1253, 1580, 1887, 1995, 2867
Sau96I GGNCC 4 cut(s) 177, 837, 1228, 1936
ScaI AGTACT 1 cut(s) 1443
SchI GAGTC 5 cut(s) 1829, 2263, 2372, 2987, 3005
ScrFI CCNGG 5 cut(s) 789, 1509, 1514, 1958, 2819
SduI GDGCHC 4 cut(s) 465, 853, 866, 1897
SfcI CTRYAG 5 cut(s) 1536, 1869, 2390, 2744, 2765
SinI GGWCC 3 cut(s) 177, 837, 1936
SmiMI CAYNNNNRTG 2 cut(s) 996, 1428
SmlI CTYRAG 5 cut(s) 26, 962, 1121, 1673, 2973
SmoI CTYRAG 5 cut(s) 26, 962, 1121, 1673, 2973
SseBI AGGCCT 1 cut(s) 2594
SsiI CCGC 5 cut(s) 474, 2416, 2452, 2829, 2832
SspI AATATT 3 cut(s) 665, 1809, 2752
SstI GAGCTC 1 cut(s) 465
StuI AGGCCT 1 cut(s) 2594
StyD4I CCNGG 5 cut(s) 787, 1507, 1512, 1956, 2817
StyI CCWWGG 3 cut(s) 156, 276, 301
TaaI ACNGT 6 cut(s) 63, 394, 409, 734, 2050, 2103
TaiI ACGT 3 cut(s) 168, 1392, 2989
TaqI TCGA 5 cut(s) 5, 1323, 1609, 3012, 3016
TatI WGTACW 3 cut(s) 1441, 1740, 2427
TauI GCSGC 2 cut(s) 2418, 2832
Tru1I TTAA 9 cut(s) 738, 963, 1109, 1224, 1253, 1580, 1887, 1995, 2867
Tru9I TTAA 9 cut(s) 738, 963, 1109, 1224, 1253, 1580, 1887, 1995, 2867
TscAI CASTG 5 cut(s) 847, 1092, 1923, 2055, 2108
TseFI GTSAC 4 cut(s) 403, 1310, 1390, 1518
TseI GCWGC 8 cut(s) 415, 451, 938, 1544, 1880, 2300, 2389, 2990
Tsp45I GTSAC 4 cut(s) 403, 1310, 1390, 1518
TspGWI ACGGA 1 cut(s) 346
TspRI CASTG 5 cut(s) 847, 1092, 1923, 2055, 2108
Van91I CCANNNNNTGG 3 cut(s) 846, 2482, 2932
Vha464I CTTAAG 1 cut(s) 962
VpaK11BI GGWCC 3 cut(s) 177, 837, 1936
XagI CCTNNNNNAGG 1 cut(s) 965
XapI RAATTY 9 cut(s) 574, 869, 1022, 1077, 1575, 2078, 2478, 2623, 2758
XceI RCATGY 2 cut(s) 80, 404
XcmI CCANNNNNNNNNTGG 1 cut(s) 90
XmiI GTMKAC 2 cut(s) 478, 1504
XmnI GAANNNNTTC 2 cut(s) 1810, 2895
ZrmI AGTACT 1 cut(s) 1443
Zsp2I ATGCAT 3 cut(s) 343, 1974, 2155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.