pycom08g16670

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0004574
Physical Location & Seq
Reverse (-)
16743139 .. 16745231
2093 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 759 bp
ATGGCTTCCGTTGTCCCCGATAACCTCACCAGGGAGCAGTACGTGTACCTGGCGAAGCTCTCCGAGCAAGCGGAGCGCTATGAGGAGATGGTCCAGTTCATGGAGAAGCTGGTCGTTGGTTCGACTCCGGCCGCCGAGCTGACCGTCGAGGAGCGCAACCTTCTCTCCGTCGCGTACAAGAACGTGATCGGCTCGCTCCGTGCCGCCTGGCGCATCGTGTCGTCGATCGAGCAGAAGGAGGAGGGCCGGAAGAACGAGGAACACGTGGTGCTTGTCAAGGAGTACAGATCCAAGGTCGAGTCCGAGCTCTCTGATGTCTGCGCTAGCATATTGAAGCTCTTGGACTCCAATCTCGTGCCGTCGGCGTCGGCCAGTGAGTCCAAGGTCTTCTATCTGAAGATGAAGGGCGACTACCACCGCTACATGGCGGAGTTCAAGTCCGCCGAGGAGAGAAAGACTGCTGCTGAGGACACCATGCTTGCTTACAAGGCTGCCCAGGACATAGCGCTTGCGGATCTGTCACCAACTCATCCAATAAGATTGGGTCTGGCACTGAATTTCTCAGTGTTCTACTATGAGATTCTCAATCAGTCTGATAAAGCTTGCAGCATGGCCAAACAGGCATTTGAGGAAGCTATTGCTGAGCTAGACACTCTGGGAGAAGAATCATACAAGGACAGCACTCTAATCATGCAACTTCTAAGGGACAACCTCACTCTTTGGACTTCTGATGCTTCGGACCAGTTGGACGAGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

253

Amino Acids

28.42

Weight (kDa)

4.74

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 17 - 242 1.6e-99 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 100
AccII CGCG 1 cut(s) 173
AciI CCGC 7 cut(s) 71, 132, 204, 418, 428, 441, 512
AclWI GGATC 2 cut(s) 282, 522
AcoI YGGCCR 3 cut(s) 129, 369, 612
AcsI RAATTY 1 cut(s) 556
AcuI CTGAAG 1 cut(s) 416
AcvI CACGTG 1 cut(s) 265
AcyI GRCGYC 1 cut(s) 365
AdeI CACNNNGTG 1 cut(s) 268
AfaI GTAC 4 cut(s) 41, 47, 176, 284
AfeI AGCGCT 2 cut(s) 77, 507
AfiI CCNNNNNNNGG 3 cut(s) 31, 100, 424
AflIII ACRYGT 2 cut(s) 42, 262
AgsI TTSAA 2 cut(s) 334, 436
AjnI CCWGG 4 cut(s) 29, 48, 206, 495
AluBI AGCT 8 cut(s) 58, 109, 139, 307, 337, 602, 635, 646
AluI AGCT 8 cut(s) 58, 109, 139, 307, 337, 602, 635, 646
Alw21I GWGCWC 1 cut(s) 309
AlwI GGATC 2 cut(s) 282, 522
Aor51HI AGCGCT 2 cut(s) 77, 507
AoxI GGCC 4 cut(s) 129, 244, 369, 612
ApeKI GCWGC 3 cut(s) 461, 491, 606
ApoI RAATTY 1 cut(s) 556
AspLEI GCGC 5 cut(s) 78, 156, 213, 323, 508
AspS9I GGNCC 3 cut(s) 91, 244, 739
AsuHPI GGTGA 2 cut(s) 19, 513
AsuNHI GCTAGC 1 cut(s) 323
AvaII GGWCC 2 cut(s) 91, 739
BalI TGGCCA 1 cut(s) 614
BanII GRGCYC 1 cut(s) 309
BarI GAAGNNNNNNTAC 2 cut(s) 395, 427
BauI CACGAG 1 cut(s) 353
BbrPI CACGTG 1 cut(s) 265
BbsI GAAGAC 1 cut(s) 379
Bbv12I GWGCWC 1 cut(s) 309
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 3 cut(s) 448, 478, 618
BccI CCATC 1 cut(s) 82
BceAI ACGGC 1 cut(s) 343
BciT130I CCWGG 4 cut(s) 31, 50, 208, 497
BfaI CTAG 2 cut(s) 324, 647
BfoI RGCGCY 2 cut(s) 79, 509
BglI GCCNNNNNGGC 1 cut(s) 620
BisI GCNGC 5 cut(s) 132, 204, 462, 492, 607
BlpI GCTNAGC 1 cut(s) 642
BlsI GCNGC 5 cut(s) 133, 205, 463, 493, 608
Bme1390I CCNGG 4 cut(s) 31, 50, 208, 497
Bme18I GGWCC 2 cut(s) 91, 739
BmgT120I GGNCC 3 cut(s) 91, 244, 739
BmrFI CCNGG 4 cut(s) 31, 50, 208, 497
BmsI GCATC 2 cut(s) 222, 721
BmtI GCTAGC 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 379
Bpu10I CCTNAGC 1 cut(s) 465
Bpu1102I GCTNAGC 1 cut(s) 642
BsaAI YACGTR 2 cut(s) 43, 265
BsaHI GRCGYC 1 cut(s) 365
BsaJI CCNNGG 5 cut(s) 30, 291, 381, 444, 495
BsaXI ACNNNNNCTCC 4 cut(s) 149, 179, 422, 452
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 100, 424
Bse1I ACTGG 3 cut(s) 94, 372, 742
BseBI CCWGG 4 cut(s) 31, 50, 208, 497
BseDI CCNNGG 5 cut(s) 30, 291, 381, 444, 495
BseGI GGATG 1 cut(s) 529
BseLI CCNNNNNNNGG 3 cut(s) 31, 100, 424
BseMII CTCAG 3 cut(s) 456, 576, 633
BseNI ACTGG 3 cut(s) 94, 372, 742
BseRI GAGGAG 4 cut(s) 98, 164, 254, 461
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 3 cut(s) 448, 478, 618
Bsh1236I CGCG 1 cut(s) 173
Bsh1285I CGRYCG 2 cut(s) 132, 228
BshFI GGCC 4 cut(s) 131, 246, 371, 614
BsiEI CGRYCG 2 cut(s) 132, 228
BsiHKAI GWGCWC 1 cut(s) 309
BsiSI CCGG 2 cut(s) 128, 247
BslFI GGGAC 1 cut(s) 719
BslI CCNNNNNNNGG 3 cut(s) 31, 100, 424
BsmFI GGGAC 1 cut(s) 719
BsnI GGCC 4 cut(s) 131, 246, 371, 614
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 4 cut(s) 186, 225, 287, 514
Bsp1720I GCTNAGC 1 cut(s) 642
BspACI CCGC 7 cut(s) 71, 132, 204, 418, 428, 441, 512
BspANI GGCC 4 cut(s) 131, 246, 371, 614
BspCNI CTCAG 3 cut(s) 457, 575, 634
BspFNI CGCG 1 cut(s) 173
BspOI GCTAGC 1 cut(s) 327
BspPI GGATC 2 cut(s) 282, 522
BsrI ACTGG 3 cut(s) 94, 372, 742
BssECI CCNNGG 5 cut(s) 30, 291, 381, 444, 495
BssMI GATC 4 cut(s) 186, 225, 287, 514
BssNI GRCGYC 1 cut(s) 365
BssSI CACGAG 1 cut(s) 353
BssT1I CCWWGG 2 cut(s) 291, 381
Bst2BI CACGAG 1 cut(s) 353
Bst2UI CCWGG 4 cut(s) 31, 50, 208, 497
Bst4CI ACNGT 1 cut(s) 145
BstACI GRCGYC 1 cut(s) 365
BstBAI YACGTR 2 cut(s) 43, 265
BstC8I GCNNGC 6 cut(s) 69, 194, 325, 480, 510, 604
BstDEI CTNAG 4 cut(s) 465, 562, 642, 701
BstF5I GGATG 1 cut(s) 529
BstFNI CGCG 1 cut(s) 173
BstH2I RGCGCY 2 cut(s) 79, 509
BstHHI GCGC 5 cut(s) 78, 156, 213, 323, 508
BstKTI GATC 4 cut(s) 189, 228, 290, 517
BstMBI GATC 4 cut(s) 186, 225, 287, 514
BstMCI CGRYCG 2 cut(s) 132, 228
BstMWI GCNNNNNNNGC 4 cut(s) 64, 73, 488, 620
BstNI CCWGG 4 cut(s) 31, 50, 208, 497
BstSCI CCNGG 4 cut(s) 29, 48, 206, 495
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 3 cut(s) 448, 478, 618
BstV2I GAAGAC 1 cut(s) 379
BstX2I RGATCY 2 cut(s) 287, 514
BstYI RGATCY 2 cut(s) 287, 514
BstZI CGGCCG 1 cut(s) 129
BsuRI GGCC 4 cut(s) 131, 246, 371, 614
BtsCI GGATG 1 cut(s) 529
BtsIMutI CAGTG 3 cut(s) 379, 551, 570
Cac8I GCNNGC 6 cut(s) 69, 194, 325, 480, 510, 604
CfoI GCGC 5 cut(s) 78, 156, 213, 323, 508
Cfr13I GGNCC 3 cut(s) 91, 244, 739
CseI GACGC 1 cut(s) 354
Csp6I GTAC 4 cut(s) 40, 46, 175, 283
CviAII CATG 5 cut(s) 100, 424, 475, 610, 691
CviQI GTAC 4 cut(s) 40, 46, 175, 283
DdeI CTNAG 4 cut(s) 465, 562, 642, 701
DpnI GATC 4 cut(s) 188, 227, 289, 516
DpnII GATC 4 cut(s) 186, 225, 287, 514
DraIII CACNNNGTG 1 cut(s) 268
EaeI YGGCCR 3 cut(s) 129, 369, 612
EagI CGGCCG 1 cut(s) 129
EciI GGCGGA 2 cut(s) 430, 443
Ecl136II GAGCTC 1 cut(s) 307
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 2 cut(s) 291, 381
Eco24I GRGCYC 1 cut(s) 309
Eco47I GGWCC 2 cut(s) 91, 739
Eco47III AGCGCT 2 cut(s) 77, 507
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 1 cut(s) 307
Eco57I CTGAAG 1 cut(s) 416
Eco72I CACGTG 1 cut(s) 265
EcoICRI GAGCTC 1 cut(s) 307
EcoRII CCWGG 4 cut(s) 29, 48, 206, 495
EcoT14I CCWWGG 2 cut(s) 291, 381
EcoT38I GRGCYC 1 cut(s) 309
ErhI CCWWGG 2 cut(s) 291, 381
FaeI CATG 5 cut(s) 103, 427, 478, 613, 694
FaqI GGGAC 1 cut(s) 719
FatI CATG 5 cut(s) 99, 423, 474, 609, 690
Fnu4HI GCNGC 5 cut(s) 132, 204, 462, 492, 607
FokI GGATG 1 cut(s) 516
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 5 cut(s) 132, 204, 462, 492, 607
FspBI CTAG 2 cut(s) 324, 647
GlaI GCGC 5 cut(s) 77, 155, 212, 322, 507
GluI GCNGC 5 cut(s) 132, 204, 462, 492, 607
HaeII RGCGCY 2 cut(s) 79, 509
HaeIII GGCC 4 cut(s) 131, 246, 371, 614
HapII CCGG 2 cut(s) 128, 247
HgaI GACGC 1 cut(s) 354
HhaI GCGC 5 cut(s) 78, 156, 213, 323, 508
Hin1I GRCGYC 1 cut(s) 365
Hin1II CATG 5 cut(s) 103, 427, 478, 613, 694
Hin6I GCGC 5 cut(s) 76, 154, 211, 321, 506
HinP1I GCGC 5 cut(s) 76, 154, 211, 321, 506
HindIII AAGCTT 1 cut(s) 600
HinfI GANTC 6 cut(s) 124, 299, 344, 377, 580, 665
HpaII CCGG 2 cut(s) 128, 247
HphI GGTGA 2 cut(s) 19, 513
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 7 cut(s) 64, 304, 313, 396, 595, 730, 739
Hpy8I GTNNAC 1 cut(s) 46
Hpy99I CGWCG 5 cut(s) 149, 173, 226, 364, 370
HpyAV CCTTC 3 cut(s) 170, 229, 397
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 3 cut(s) 42, 183, 264
HpyCH4V TGCA 2 cut(s) 606, 694
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 73, 488, 620
HpyF3I CTNAG 4 cut(s) 465, 562, 642, 701
HpySE526I ACGT 3 cut(s) 42, 183, 264
Hsp92I GRCGYC 1 cut(s) 365
Hsp92II CATG 5 cut(s) 103, 427, 478, 613, 694
HspAI GCGC 5 cut(s) 76, 154, 211, 321, 506
Kzo9I GATC 4 cut(s) 186, 225, 287, 514
LmnI GCTCC 4 cut(s) 34, 73, 151, 201
Lsp1109I GCAGC 3 cut(s) 448, 478, 618
LweI GCATC 2 cut(s) 222, 721
MaeI CTAG 2 cut(s) 324, 647
MaeII ACGT 3 cut(s) 42, 183, 264
MaeIII GTNAC 1 cut(s) 519
MalI GATC 4 cut(s) 188, 227, 289, 516
MboI GATC 4 cut(s) 186, 225, 287, 514
MboII GAAGA 4 cut(s) 262, 379, 409, 674
MflI RGATCY 2 cut(s) 287, 514
MhlI GDGCHC 1 cut(s) 309
MlsI TGGCCA 1 cut(s) 614
MluCI AATT 1 cut(s) 556
MluNI TGGCCA 1 cut(s) 614
MlyI GAGTC 4 cut(s) 118, 308, 338, 386
MmeI TCCRAC 1 cut(s) 726
Mox20I TGGCCA 1 cut(s) 614
MscI TGGCCA 1 cut(s) 614
Msp20I TGGCCA 1 cut(s) 614
MspI CCGG 2 cut(s) 128, 247
MspR9I CCNGG 4 cut(s) 31, 50, 208, 497
MvaI CCWGG 4 cut(s) 31, 50, 208, 497
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 4 cut(s) 64, 73, 488, 620
NdeII GATC 4 cut(s) 186, 225, 287, 514
NheI GCTAGC 1 cut(s) 323
NlaIII CATG 5 cut(s) 103, 427, 478, 613, 694
NmeAIII GCCGAG 2 cut(s) 160, 469
NmuCI GTSAC 1 cut(s) 519
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PfeI GAWTC 2 cut(s) 580, 665
PflMI CCANNNNNTGG 1 cut(s) 100
PkrI GCNGC 5 cut(s) 133, 205, 463, 493, 608
Ple19I CGATCG 1 cut(s) 228
PleI GAGTC 4 cut(s) 118, 307, 338, 385
PmaCI CACGTG 1 cut(s) 265
PmlI CACGTG 1 cut(s) 265
PpsI GAGTC 4 cut(s) 118, 307, 338, 385
Ppu21I YACGTR 2 cut(s) 43, 265
Psp124BI GAGCTC 1 cut(s) 309
Psp6I CCWGG 4 cut(s) 29, 48, 206, 495
PspCI CACGTG 1 cut(s) 265
PspGI CCWGG 4 cut(s) 29, 48, 206, 495
PspPI GGNCC 3 cut(s) 91, 244, 739
PsuI RGATCY 2 cut(s) 287, 514
PvuI CGATCG 1 cut(s) 228
RsaI GTAC 4 cut(s) 41, 47, 176, 284
RsaNI GTAC 4 cut(s) 40, 46, 175, 283
SacI GAGCTC 1 cut(s) 309
SatI GCNGC 5 cut(s) 132, 204, 462, 492, 607
Sau3AI GATC 4 cut(s) 186, 225, 287, 514
Sau96I GGNCC 3 cut(s) 91, 244, 739
SchI GAGTC 4 cut(s) 118, 308, 338, 386
ScrFI CCNGG 4 cut(s) 31, 50, 208, 497
SduI GDGCHC 1 cut(s) 309
SfaNI GCATC 2 cut(s) 222, 721
SinI GGWCC 2 cut(s) 91, 739
Sse9I AATT 1 cut(s) 556
SsiI CCGC 7 cut(s) 71, 132, 204, 418, 428, 441, 512
SspMI CTAG 2 cut(s) 324, 647
SstI GAGCTC 1 cut(s) 309
StyD4I CCNGG 4 cut(s) 29, 48, 206, 495
StyI CCWWGG 2 cut(s) 291, 381
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 3 cut(s) 45, 186, 267
TaqI TCGA 5 cut(s) 122, 147, 224, 228, 297
TasI AATT 1 cut(s) 556
TatI WGTACW 1 cut(s) 282
TauI GCSGC 2 cut(s) 134, 206
TfiI GAWTC 2 cut(s) 580, 665
TscAI CASTG 3 cut(s) 379, 558, 570
TseFI GTSAC 1 cut(s) 519
TseI GCWGC 3 cut(s) 461, 491, 606
Tsp45I GTSAC 1 cut(s) 519
TspDTI ATGAA 2 cut(s) 88, 416
TspGWI ACGGA 2 cut(s) 157, 188
TspRI CASTG 3 cut(s) 379, 558, 570
Van91I CCANNNNNTGG 1 cut(s) 100
VpaK11BI GGWCC 2 cut(s) 91, 739
XapI RAATTY 1 cut(s) 556
XspI CTAG 2 cut(s) 324, 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.