pycom08g17530

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012629
Physical Location & Seq
Reverse (-)
17750246 .. 17758548
8303 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 7983 bp
ATGCAAACAATTTTAGAAAACTGCAATAACAAGAGTTCATTTGATGGCTTAGAGCATTTCAAGCTCCTACTTGCATCAACGGATCCCGAGGTCCTTATAAATGCATTAGAAACTCTTTCCGCACTTGTGAAAATAAATCCCTCCAAGCTGCATGCCAGTGGGAAGATGATCGGTTGTGGTTCTGTAAACAGCTATCTCCTGTCTCTGGCACAGGGCTGGGGCAGCAAGGAAGAGGGATTGGGTTTATATTCATGTGTTATGGCAAATGAGACTAACCAAGACGACGGGCTCAATTTGTTCCCATCTGATGTAGAAAATGATTCTGACAAGTTGCAGTGCCGAATCGGTTCCACGCTCTATTTTGAATTGCATGGGAATGCCCAAAGTACAGAGGAGAGCTCTAGCAATGTAAATAATTCTTCGAGTTTGGGGGTTATAAACATTCCAGATTTGCATTTGCAAAAGGAGGATGATTTAAAGCTAATGAAACGATATATAGAAGAGTACAGGGTACCTTCTGAACTGCGGTTCTCACTGCTGACAAGAATCAGATATTCTCGTGCCTTCCGTTCTCCAAAAATATGTAGGCTGTACAGCAGGATTTGCCTTCTTGCATTTGTTGTGCTCGTTCAGTCTAGTGATGCCAGTGCAGAACTGAATTCCTTCTTTGCTAATGAGCCAGAGTTTACAAATGAATTAATCAGAATTGTGCGGTCCGAAGAATCGGTGTCTGGGACCATCAGAACGCAGGCAATGCTTGCCTTGGGTGCTCAGTTAGCTGCATATTCAGCGTCTCATGAGCGGGCACGAATCTTGAGTGTATCTAGTATCAGTTTTGCAGGGGGAAATCGTATGATACTCCTGAATGTGCTACAGAGAGCTGTTAGATCATTAAAAAACTCCAATGATCCAACTTCTCTTGCTTTTGTTGAAGCACTTCTTCAGTTCTATCTGCTCCATGTGGTTTCATCATCAGCTACAGGGAGTAATATTAGAGGGTCAGGCATGGTTCCAACATTCTTGCCTCTTTTGGAGGATTCTGACCCTTTGCATTTGCATCTAGTCTGTTTTGCTGTCAAAACTCTACAAAAGCTCATGGATTACAGTAGTTCAGCTGTGTCTCTGTTTAAAGAGTCGGGGGGAGTTGAGCTTTTGTCTCAGAGACTACAGATAGAGGTACACAGAGTTATTGGTTTGGCCGGCAATAATGATAACTCAGTGGTCATCGGAGAAAGCTCGAGATACAGTGACAACCAGCTGTACTCACAAAAGAGGCTCATTAAAGCTTCGTTGAAGGCACTTGGGTCTGCTACATATGCCGCTGGGAATTCCTCCAGAGTGCAACACTCCCATGACAGTTCTTTACCTGCAACTCTCTCTCTGATATTTGGGAATGTGGACAAATTTGGTGGTGACATTTATTACTCTGCAGTGACTGTTTTGAGTGAAACTATCCACAAAGACCCAACTTGCTTTTCTGCTTTGCATGAAATGGGTCTGCCAGATGCATTTATATCCTCAGTTGTAGCTGGAATTCTTCCCTCTGCAAAGGCTCTTACATGTGTACCTAACGGTCTTGGTGCTATTTGTCTTAATGCCAAAGGCTTGGAGGCTGTGAAGGAAAGTTTTGCATTACGCTTTCTTGTTGACATCTTCACGAGCAAGAAATATGTTACTGCAATGAATGAAGCTATTGTTCCTTTGGGAAATGCAGTGGAAGAGCTTTTGCGCCATGTATCTTCGCTGCGAAGCACTGGTGTTGATATCATCCTTGAGATAATTGATAAAATTGCTTCCTTCACTGACAGCCACAATACAGGGGCAGCAGGAAAGGCAAATGCGAGTACTGCAATGGAAATGGATTCTGAAGAGAGAGAAAATGAAGGTAGTTGCTGTCTGGTTGATTCTGTAGATTCAGCTGCAGATGGGATCGGTAATGAGCAGTTCATTCAATTATCCCTCTTTCATTTGATGATACTGGTCCACCGAACAATGGAGAATTCCGAAACATGTCGGTTGTTTGTCGAGAAGTCAGGAATTGATGCATTACTTAAGCTTCTTCTCCAACCTGCTATTGTACAATCATCCGATGGGATGTCTATTGCTTTGCACAGTACTATGGTTTTCAAGGGTTTTACTCAACATCACTCTGCTGCCCTTGCCCGTGCATTTTGCTCCTCCCTTGGGGACCACCTAAAGAAAGCTTTGTCTGGTTTTGGGGCGGTTTCAGGATCCTTTTTGCTGGAACCTAGGATGGCATCAGATGGTGGAATTTTTTCTTCTCTGTTTCTTGTTGAGTTCCTTTTGTTTATTGCTGCCTCAAAGGACAACCGCTGGGTAACTGCATTGCTTACGGAATTTGTAAATGGTAGCAAGGATGTTCTTGAAGATATAGGACATGTCCATCGTGAAGTTTTATGGCAGATTGCTCTCTTAGAAGATACAAAATTGGACGTGGAGGATGATAATGCTGGTTCTACCAGTGAGTCACAACAGTCAGAAACAAATACAAGTGAAACTGAAGAGCAAAGGTTCAACTCTTTTAGGCAGTTCCTTGATCGATTGCTCAGAAGGAGGACTTCTGGGTGGAGCATTGAATCCCAGTTTCTGGACCTTATTAGCCTATACCGTGACCTTGGTCGCGCCACTAGTTCTCAACAAAGAACAAATTCTGATGGTCATTCAAATCTTCGGATTGGATCCAGTCATCAGTTCCATCATTCTGGGTCTTCTGATGCTGTTGGCCCTCTTAATAAAAAGGAGTATGACCAGCAGAGGTCATATTATACTTCTTGTTGTGACATGGTGAGATCACTTTCTTTTCACATTACCCATCTGTTTCAAGAGCTGGGGAAGGTCATGTCGGTACCTTCTCGTCGACGGGATGATATTGTGAATGTTTCTCCTGCTGCAAAATCTGTGGCTTCTACTTTTGCGTCCATTGCATTTGATCACTTGAACTTCGAAGGGCATGCTAATTCTTCTGAATCCAAGGCTTCCATATCAACAAAGTGTCGGTACTTTGGTAAAGTTATTGATTTCATTGATGTCAGTCTACTGGAGAGAGCTGATTCGTGTAATGCTGTTTTGCTAAATTGCTTGTATGGTCATGGAGTTGTCCAGTCAGTTTTGAAGACATTTGTAGCTACCAGTGAATTGCTCTTCACAGTCAGGGCTCCTGCATCCCCCATGGAAACGGATGATGGGAATTCAAAGCAGGATGAAAGGGAAGATATTGACCATTCATGGATTTATGGTCCTTTAGCTAGCTATGGTAAACTTATGGATCACCTGGTAACATCATCTTTCATTTTATCTCCATTCACTAAACACTTGCTAGCACAACCCCTGGCAAATGGAAATATTCCTTTCCCGCGTGATGCTGAGACCTTTGTAAAGGTCCTCCAGTCCATGGTGCTGAAGGCAATTCTTCCTCTTTGGACTCATCCTCAGTTTGTTGATTGTAGTTATGATTTTACTGGTACTGTTATTTCTATAATCAGGCATATATACTCTGGGGTTGAAGTGAAGAATGTTAGTAGCAGCAACACTACCCGTATTACTGGCCCTCCTCCTAATGAGACAACTATTTCAATGATTGTGGAGATGGGCTTTACCAGGTCTAGGGCAGAAGAAGCTCTGAGGCAAGTTGGATCAAATAGTGTGGAGTTGGCAATGGAGTGGTTGTTTTCCCATCAAGAGGAGGCTGAAGAAGACAATGAACTTGCTCGCGCACTTGCTATGTCTTTGGGTAATGAATCAGACACTAAGGAAGCTGTTGCAAATGACAATGCTCAGCAGCTTGAAGAAGAGATGGTCCAACTTCCTCCTGTTGAGGAGTTGTTATCTACATGCGCAAAGCTTCTGCAGATGAAGGAACCTCTAGCTTTTCCAGTTCGAGACCTGCTAGTAATGATATGCTCCCAAAATGATGGTCAATATCGGCCTAATATAATCTCTTTCTTTGTTGAGCAGATTAAAGAATCTAGTTTGAGTTTTGACAGTGGGAACAGTACTTTGCTATCTGCTCTTTTTCACGTTCTTGCTTTAATTCTTCAAGAAGACGCAATTGCACGTGAACTTGCCTCGAAGAATGGTCTTGTGAAAGTTGTATCAGATCTACTCTTCCAGTGGGATTCTGGTTCAGTTGGCAGGGAGAAACGTGAAGTTCCAAGGTGGGTAACAACAGCTTTTCTTGCTGTCGATCGGTTGTTGCAGGTGGATCAAAAACTGAACCCTGAAATTGTAGAGCAGTTGAAGAAGGATAGTGTCAGTAGCCACCAAACACCACTTAGCATTGATGAGGACAAGCAAAATAAGTTGCAGTCTGCATTGGGATTATCTTCAAAGCATATAGAAATGAAAGAGCAGAAGAGGCTTATTGAGATAGCTTGTTCTTGCATCAGGAACCAGCTTCCTTCTGAAACAATGCATGCTGTTCTGCAGCTTTGCTCTACTCTCACAAAAACTCATGCTGTTGCTGTTCAATTTCTTGATGTCGGGGGTTTAAGTTTACTGCTTTCTTTGCCGACAAGTTGCCTCTTTCCTGGGTTTGACAATATTGCTGCTACCATTATCCGTCATGTTCTGGAAATGATCGATCGATCTCAAACTCTTCAGCAGGCAATGGAATTTGAGATAAGGCACAGCCTTGTGGCAGCTGCCAACAGGCATTCTAACGGTAGGGTGTCTCCCCGCAATTTTCTATTAAGTTTAAGTTCTGCAATTTCTCGTGATCCGGTGATATTTATGCGTGCTGCTCAGTCTATTTGTCAAGTTGAGATGGTTGGTGAAAGGCCTTACATTGTTTTGCTGAAAGATCGCGAGAAAGATAAAAGCAAGGAAAAGGAAAAAGAGAAGGATAAATCTTTGGATAAAGACAAAACACTAATGGCTGATAGTAAAGCTGCTCTGGGTAATATAAATTCAGTAGCTTCTGGCAATGGACATGGCAAGGTTCCTGATTCAAATTCAAAGAGTGCAAAAGTTCATCGAAAATACCCCCAGAGTTTTGTCTCTGTGATTGAGCTTCTTCTGGATTCTGTGTGTACTTATGTCCCGCTGACTAAGGATAATGTTGTCACTGATGCACCACCTTCAAATGACATGGATATTGATGTTGCTGCTATAAAAGGGAAGGGTAAAGCAATTGCTTCAGTGTCTGAAGATAACGAAACTTGTACCCAAGAAGCTCCTGCATCTCTTGCAAAGGTTGTATTTGTTTTGAAGCTTCTAACTGAGATCTTATCAATGTATGCTTCGTCTGCGCATGTTCTGCTTCGGAAAGATGCTGAAATTAGCAGTTGCAGGGCCCCCAGTCAAAAGGGTCCTACTGCTGTATGCACTGGTGGAATATTTAACCATGTTCTTCATAAGTTTCTTCCGTATTCACGCAGTGCCAAGAAGGAAAAGAAAGTTGATGGTGACTGGAGGCACAAATTAGCAAGTAGGGCTAGCCAGTTTTTAGTTGCATCCTGTGTTCGTTCTTCTGAGGCAAGGAAGAGAGTATTTACTGAGATCAGTTTAATATTTAATGACTTTGTAGAATCATGCAATGGTTTCCGGCCACCTAACAACGAAATACAAGCTTTTTGTGATCTACTTAATGATGTTCTGGCTGCCCGTACTCCTACAGGTTCGTACATTTCTGCGGAAGCTTCTGTCACTTTTATAGATGTTGGTCTGATTGGGGCATTGACTCGAACTCTCCAAATGCTGGACTTGGACCATGCTGACTCACCTAAAGTTGTTACTGGTCTTCTCAAAGCTCTTGAGTTGGTAACCAAAGAACATGTTCATTCTGCTGACTCAAATTCTGGTAAGGATGACAATTCTACCAAACCTCCTGATCATAATCAAGCTGGAACGACAGACAATATTGTTGAGAGATCCGAATCTATGGAAACACCATCCCAGTCCCACCATGATTCTGGTACAGCTGAGCCCATTGAGTCTTATAACGCGGTCCAATCTTTTGGTGGTTCTGTGGCTGTGACTGATGATATGGAACATGACCAAGATCTCGATGGAGGTTTTGCTCCTGCAAATGAAGATGAATATATGCATGAAAATTCGGAGGAAACAAGGGGTCTTGAAAATGGTATTGATACTATGGGTATACGGTTTGAAATTCAACCTCACGAGCAAGAGAATCTTGATGACGACGACGACGACGATGAAGATATGTCTGAGGATGATGCAGATGAAGTAGACGATGACGAAGATGATGACGATGAGGAGCATAATGATCTGGAAGATGACGTGCATCACCTGCCACATCCTGACACTGACCAAGATGATCATGAGATTGATGATGATGAGTTTGATGAGGAAGTATTAGAGGAAGATGATGAAGAAGATGACGATGAGGAGGATGGAGTCATACTGAGGCTGGAGGAAGGCATAAATGGGATTAATGTTTTTGACCGTATTGAAGTTTTTGGTAGAGATCCTGGTTTTCCTAATGAAGGACGTCAGGTGATGCCAGTTGAAGTTTTTGGTTCTAGACGTCAAGGGCGCACTACTTCTATTTACAGTCTTTTGGGAAGAACTGGCGAGAATGCCACTCCATCAAGGCATCCTCTTCTGGTTGGACCGTTGTCTCTATCGTCAGCCCCGCCTAGACAATCAGAGAATACCCGTGATATGGTGTTACCAGATTTAAACTCGGAGGTCACTTCGTCAAGATTGGATAATATTTTCCGTTCATTGAGGAATGGGCGTCATGGACATCGACTGAACTTGTGGATGGATGATAACCAGCAAGGTGGTGGATCAAATCCAAGTTCAGTGCCACAAGGACTTGAGGATTTGCTTGTGTCCCAATTAAGGCGACCGATGGCTGACAAGACATCTGAAGAGAATAATACAAAAGCGGTGGATTCTCAGAACACAGCTGAAGCTTTAGAGTTACAAGAACCACAAACAGGTGTCAGGCCTGAAATTCCGGTTGAGAACAATGTCACTATTGAAAGTGGAAGCCTGCCCCCTCCTGAGACAACTGATGACTCTGGAAATGCTGATTTGAGACCAACTACAGTGAGCGAATCTCTACAATCAATGGACATGTCTAGCATGCATCCACAGTCCGTTCAGATGCAGTTCGAACCCAATGATGCAGCTGTGAGGGATGTAGAAGCTGTGAGCCAAGAAAGTGGTGGAAGTGGAGCAACTTTAGGGGAGAGCCTTCGTAGCCTTGATGTTGAAATTGGGAGTGCTGATGGTCATGATGATGGAGCAGAGAGGCAAGGTTCTTCTGATCGAATGCCACTGGGTGATTCACAGGCAACTCGAGCAAGAAGAACAAATGTATCTTCCGGGAATTCTGCCACTGTCAGTGCAAGAGATGTTTCTCTACATAGCGTAACAGAAGTTTCTGAAAATTCTAGCCGTGAGGCAGATCAGGAGGGTCCAGCAGCAGAACAGCAACTTAACAGTGATGCCGGTTCTGGTGCTATTGACCCTGCTTTCTTGGATGCCCTTCCTGAGGAGTTGCGTGCTGAAGTTCTCTCGGCACAGCAGGGTCAAGCAGCTCCACAGTCAAATGCTGAGCCACAAAATGCTGGAGATATTCCAACCACCGGTCTCCCCGATCCAGAATTCCTTGCTGCCCTTCCTCCTGATATCAGAGCAGAAGTTTTAGCCCAACAGCAAGCTCAAAGGCTACATCAGTCTCAGGAACTTGAAGGTCAACCAGTTGAAATGGACACAGTCTCAATAATTGCAACATTTCCTTCAGAGTTGCGTGAAGAAGTTCTCTTGACTTCATCCGATGCTATACTTGCCAATCTTACCCCTGCACTAGTTGCGGAGGCAAATCTGTTACGTGAAAGATTTGCCCATCGTTATAATCGTACCCTGTTTGGTATGTATCCGAGAAACCGTAGGGGTGAGACTTCTAGACCAGGTGAAGGCATTGGATCTAGCCTGGACAGGATTGGGGGAAGCATTGCCTCACGTATCGATCAATTGGTGCCAAGGTAG

Protein Analysis

2661

Amino Acids

291.43

Weight (kDa)

4.87

Isoelectric Point (pI)

49.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF908 PF06012 2 - 295 5.2e-27 Domain of Unknown Function (DUF908)
DUF913 PF06025 358 - 690 1.3e-63 Domain of Unknown Function (DUF913)
UBA PF00627 1192 - 1229 5.1e-09 UBA/TS-N domain
UBA_7 PF22562 1193 - 1239 1.1e-14 UBA-like domain
UBM PF14377 2486 - 2508 3.3e-07 Ubiquitin binding region
UBM PF14377 2531 - 2555 2.2e-09 Ubiquitin binding region
UBM PF14377 2563 - 2594 7.2e-07 Ubiquitin binding region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 98, 437, 5958, 7848
AarI CACCTGC 2 cut(s) 4207, 6279
AatII GACGTC 2 cut(s) 6475, 6511
Acc16I TGCGCA 2 cut(s) 3856, 5268
Acc36I ACCTGC 5 cut(s) 1375, 2077, 3912, 4207, 6279
Acc65I GGTACC 2 cut(s) 511, 2866
AccB1I GGYRCC 3 cut(s) 511, 2866, 7972
AccB7I CCANNNNNTGG 3 cut(s) 2608, 3412, 5716
AccBSI CCGCTC 1 cut(s) 802
AccI GTMKAC 4 cut(s) 2878, 3055, 6118, 6210
AccII CGCG 5 cut(s) 2643, 3376, 3732, 4824, 5963
AcoI YGGCCR 2 cut(s) 1197, 5564
AcvI CACGTG 1 cut(s) 4076
AcyI GRCGYC 3 cut(s) 6472, 6508, 6721
AdeI CACNNNGTG 2 cut(s) 5396, 7274
AflII CTTAAG 1 cut(s) 2051
AflIII ACRYGT 5 cut(s) 1559, 2009, 2398, 5791, 7065
AgeI ACCGGT 1 cut(s) 7580
AhlI ACTAGT 2 cut(s) 2648, 7801
AjiI CACGTC 2 cut(s) 2455, 6262
AjnI CCWGG 7 cut(s) 3291, 3348, 3617, 4546, 6451, 7903, 7926
AjuI GAANNNNNNNTTGG 2 cut(s) 1459, 1491
Alw21I GWGCWC 3 cut(s) 401, 627, 772
AlwNI CAGNNNCTG 3 cut(s) 1436, 2608, 5162
Ama87I CYCGRG 3 cut(s) 86, 1237, 7290
AoxI GGCC 8 cut(s) 1197, 2743, 3565, 3944, 4796, 5310, 5564, 6935
ApaI GGGCCC 1 cut(s) 5314
ArsI GACNNNNNNTTYG 2 cut(s) 3919, 3951
AseI ATTAAT 2 cut(s) 698, 6414
AsiGI ACCGGT 1 cut(s) 7580
Asp700I GAANNNNTTC 7 cut(s) 346, 662, 936, 2275, 2668, 5793, 7752
Asp718I GGTACC 2 cut(s) 511, 2866
AspA2I CCTAGG 1 cut(s) 2249
AspLEI GCGC 6 cut(s) 1731, 2645, 3734, 3857, 5269, 6519
AsuC2I CCSGG 1 cut(s) 7318
AsuII TTCGAA 2 cut(s) 2964, 7104
AsuNHI GCTAGC 3 cut(s) 3266, 3337, 5453
AvaI CYCGRG 3 cut(s) 86, 1237, 7290
AvrII CCTAGG 1 cut(s) 2249
AxyI CCTNAGG 1 cut(s) 7485
BaeGI GKGCMC 2 cut(s) 808, 5314
BaeI ACNNNNGTAYC 6 cut(s) 1548, 1581, 3010, 3043, 7293, 7326
BamHI GGATCC 3 cut(s) 82, 2231, 2699
BanI GGYRCC 3 cut(s) 511, 2866, 7972
BanII GRGCYC 5 cut(s) 291, 401, 3178, 5314, 5946
BauI CACGAG 4 cut(s) 558, 1657, 4731, 6140
BbrPI CACGTG 1 cut(s) 4076
BbsI GAAGAC 5 cut(s) 2721, 3140, 3720, 4068, 5750
Bbv12I GWGCWC 3 cut(s) 401, 627, 772
BceAI ACGGC 1 cut(s) 7374
BcgI CGANNNNNNTGC 2 cut(s) 2954, 2988
BciT130I CCWGG 7 cut(s) 3293, 3350, 3619, 4548, 6453, 7905, 7928
BciVI GTATCC 1 cut(s) 7881
BclI TGATCA 3 cut(s) 2950, 5848, 6298
BcnI CCSGG 1 cut(s) 7318
BcuI ACTAGT 2 cut(s) 2648, 7801
BfrI CTTAAG 1 cut(s) 2051
BfuAI ACCTGC 5 cut(s) 1375, 2077, 3912, 4207, 6279
BfuI GTATCC 1 cut(s) 7881
BglII AGATCT 3 cut(s) 4117, 5241, 6019
BlnI CCTAGG 1 cut(s) 2249
BlpI GCTNAGC 3 cut(s) 3795, 5940, 7548
BmcAI AGTACT 3 cut(s) 1846, 2116, 4015
Bme1390I CCNGG 8 cut(s) 3293, 3350, 3619, 4548, 6453, 7318, 7905, 7928
BmeT110I CYCGRG 3 cut(s) 86, 1237, 7290
BmgBI CACGTC 2 cut(s) 2455, 6262
BmrFI CCNGG 8 cut(s) 3293, 3350, 3619, 4548, 6453, 7318, 7905, 7928
BmrI ACTGGG 4 cut(s) 2596, 5310, 5908, 7280
BmtI GCTAGC 3 cut(s) 3270, 3341, 5457
BmuI ACTGGG 4 cut(s) 2596, 5310, 5908, 7280
BoxI GACNNNNGTC 2 cut(s) 2637, 6598
BpiI GAAGAC 5 cut(s) 2721, 3140, 3720, 4068, 5750
BplI GAGNNNNNCTC 4 cut(s) 383, 415, 7033, 7065
BpmI CTGGAG 6 cut(s) 1318, 3080, 3389, 5449, 6413, 7584
Bpu1102I GCTNAGC 3 cut(s) 3795, 5940, 7548
Bpu14I TTCGAA 2 cut(s) 2964, 7104
BpuEI CTTGAG 4 cut(s) 835, 1793, 5792, 6824
BpuMI CCSGG 1 cut(s) 7318
Bsa29I ATCGAT 4 cut(s) 2560, 4599, 4603, 7962
BsaAI YACGTR 3 cut(s) 4076, 7826, 7958
BsaBI GATNNNNATC 2 cut(s) 5853, 5893
BsaHI GRCGYC 3 cut(s) 6472, 6508, 6721
BsaI GGTCTC 4 cut(s) 3380, 3894, 7021, 7589
BsaWI WCCGGW 3 cut(s) 4738, 6946, 7580
BsaXI ACNNNNNCTCC 6 cut(s) 3553, 3583, 3671, 3701, 5827, 5857
Bse118I RCCGGY 3 cut(s) 1199, 7442, 7580
Bse21I CCTNAGG 1 cut(s) 7485
Bse8I GATNNNNATC 2 cut(s) 5853, 5893
BseBI CCWGG 7 cut(s) 3293, 3350, 3619, 4548, 6453, 7905, 7928
BseCI ATCGAT 4 cut(s) 2560, 4599, 4603, 7962
BseJI GATNNNNATC 2 cut(s) 5853, 5893
BseRI GAGGAG 8 cut(s) 407, 2167, 3561, 3716, 3851, 6251, 6383, 7502
BseSI GKGCMC 2 cut(s) 808, 5314
BseYI CCCAGC 4 cut(s) 216, 1322, 2334, 2848
BsgI GTGCAG 2 cut(s) 669, 7782
Bsh1236I CGCG 5 cut(s) 2643, 3376, 3732, 4824, 5963
Bsh1285I CGRYCG 3 cut(s) 4207, 4603, 6836
BshFI GGCC 8 cut(s) 1199, 2745, 3567, 3946, 4798, 5312, 5566, 6937
BshNI GGYRCC 3 cut(s) 511, 2866, 7972
BshTI ACCGGT 1 cut(s) 7580
BshVI ATCGAT 4 cut(s) 2560, 4599, 4603, 7962
BsiEI CGRYCG 3 cut(s) 4207, 4603, 6836
BsiHKAI GWGCWC 3 cut(s) 401, 627, 772
BsiHKCI CYCGRG 3 cut(s) 86, 1237, 7290
BsiSI CCGG 7 cut(s) 1200, 4739, 5563, 6947, 7317, 7443, 7581
BslFI GGGAC 5 cut(s) 748, 2201, 5042, 5902, 6805
BsmBI CGTCTC 1 cut(s) 798
BsmFI GGGAC 5 cut(s) 748, 2201, 5042, 5902, 6805
BsmI GAATGC 4 cut(s) 382, 4672, 6565, 7269
BsnI GGCC 8 cut(s) 1199, 2745, 3567, 3946, 4798, 5312, 5566, 6937
Bso31I GGTCTC 4 cut(s) 3380, 3894, 7021, 7589
BsoBI CYCGRG 3 cut(s) 86, 1237, 7290
Bsp119I TTCGAA 2 cut(s) 2964, 7104
Bsp120I GGGCCC 1 cut(s) 5310
Bsp1286I GDGCHC 8 cut(s) 291, 401, 627, 772, 808, 3178, 5314, 5946
Bsp1407I TGTACA 2 cut(s) 591, 2077
Bsp1720I GCTNAGC 3 cut(s) 3795, 5940, 7548
Bsp19I CCATGG 2 cut(s) 3189, 3411
Bsp68I TCGCGA 1 cut(s) 4824
BspANI GGCC 8 cut(s) 1199, 2745, 3567, 3946, 4798, 5312, 5566, 6937
BspDI ATCGAT 4 cut(s) 2560, 4599, 4603, 7962
BspFNI CGCG 5 cut(s) 2643, 3376, 3732, 4824, 5963
BspHI TCATGA 3 cut(s) 796, 6301, 7225
BspMAI CTGCAG 4 cut(s) 1432, 1924, 3870, 4446
BspMI ACCTGC 5 cut(s) 1375, 2077, 3912, 4207, 6279
BspOI GCTAGC 3 cut(s) 3270, 3341, 5457
BspQI GCTCTTC 3 cut(s) 1713, 2517, 3167
BspT104I TTCGAA 2 cut(s) 2964, 7104
BspT107I GGYRCC 3 cut(s) 511, 2866, 7972
BspTI CTTAAG 1 cut(s) 2051
BspTNI GGTCTC 4 cut(s) 3380, 3894, 7021, 7589
BsrBI CCGCTC 1 cut(s) 802
BsrFI RCCGGY 3 cut(s) 1199, 7442, 7580
BsrGI TGTACA 2 cut(s) 591, 2077
BssAI RCCGGY 3 cut(s) 1199, 7442, 7580
BssNAI GTATAC 1 cut(s) 6119
BssNI GRCGYC 3 cut(s) 6472, 6508, 6721
BssSI CACGAG 4 cut(s) 558, 1657, 4731, 6140
BssT1I CCWWGG 9 cut(s) 762, 2182, 2249, 2635, 2991, 3189, 3411, 4172, 7976
Bst1107I GTATAC 1 cut(s) 6119
Bst2BI CACGAG 4 cut(s) 558, 1657, 4731, 6140
Bst2UI CCWGG 7 cut(s) 3293, 3350, 3619, 4548, 6453, 7905, 7928
BstACI GRCGYC 3 cut(s) 6472, 6508, 6721
BstAFI CTTAAG 1 cut(s) 2051
BstAPI GCANNNNNTGC 7 cut(s) 603, 754, 758, 5204, 5275, 6271, 7805
BstAUI TGTACA 2 cut(s) 591, 2077
BstBAI YACGTR 3 cut(s) 4076, 7826, 7958
BstBI TTCGAA 2 cut(s) 2964, 7104
BstDSI CCRYGG 2 cut(s) 3189, 3411
BstEII GGTNACC 1 cut(s) 5779
BstENI CCTNNNNNAGG 2 cut(s) 6465, 7484
BstFNI CGCG 5 cut(s) 2643, 3376, 3732, 4824, 5963
BstHHI GCGC 6 cut(s) 1731, 2645, 3734, 3857, 5269, 6519
BstMCI CGRYCG 3 cut(s) 4207, 4603, 6836
BstNI CCWGG 7 cut(s) 3293, 3350, 3619, 4548, 6453, 7905, 7928
BstPAI GACNNNNGTC 2 cut(s) 2637, 6598
BstPI GGTNACC 1 cut(s) 5779
BstSCI CCNGG 8 cut(s) 3291, 3348, 3617, 4546, 6451, 7316, 7903, 7926
BstSLI GKGCMC 2 cut(s) 808, 5314
BstUI CGCG 5 cut(s) 2643, 3376, 3732, 4824, 5963
BstV2I GAAGAC 5 cut(s) 2721, 3140, 3720, 4068, 5750
BstX2I RGATCY 9 cut(s) 82, 2231, 2699, 4117, 5241, 5888, 6019, 6448, 7919
BstXI CCANNNNNNTGG 7 cut(s) 1606, 2723, 3932, 5930, 5975, 6767, 7154
BstYI RGATCY 9 cut(s) 82, 2231, 2699, 4117, 5241, 5888, 6019, 6448, 7919
BstZ17I GTATAC 1 cut(s) 6119
Bsu15I ATCGAT 4 cut(s) 2560, 4599, 4603, 7962
Bsu36I CCTNAGG 1 cut(s) 7485
BsuI GTATCC 1 cut(s) 7881
BsuRI GGCC 8 cut(s) 1199, 2745, 3567, 3946, 4798, 5312, 5566, 6937
BsuTUI ATCGAT 4 cut(s) 2560, 4599, 4603, 7962
BtgI CCRYGG 2 cut(s) 3189, 3411
BtrI CACGTC 2 cut(s) 2455, 6262
BtsI GCAGTG 5 cut(s) 341, 533, 1437, 1719, 5401
BtuMI TCGCGA 1 cut(s) 4824
BveI ACCTGC 5 cut(s) 1375, 2077, 3912, 4207, 6279
CaiI CAGNNNCTG 3 cut(s) 1436, 2608, 5162
CciI TCATGA 3 cut(s) 796, 6301, 7225
CfoI GCGC 6 cut(s) 1731, 2645, 3734, 3857, 5269, 6519
Cfr10I RCCGGY 3 cut(s) 1199, 7442, 7580
ClaI ATCGAT 4 cut(s) 2560, 4599, 4603, 7962
CpoI CGGWCCG 1 cut(s) 714
CseI GACGC 4 cut(s) 780, 2925, 4073, 6710
CsiI ACCWGGT 3 cut(s) 3291, 3617, 7903
CspAI ACCGGT 1 cut(s) 7580
CspI CGGWCCG 1 cut(s) 714
DraI TTTAAA 3 cut(s) 477, 1129, 6663
DraIII CACNNNGTG 2 cut(s) 5396, 7274
EaeI YGGCCR 2 cut(s) 1197, 5564
Ecl136II GAGCTC 1 cut(s) 399
Eco130I CCWWGG 9 cut(s) 762, 2182, 2249, 2635, 2991, 3189, 3411, 4172, 7976
Eco147I AGGCCT 2 cut(s) 4798, 6937
Eco24I GRGCYC 5 cut(s) 291, 401, 3178, 5314, 5946
Eco31I GGTCTC 4 cut(s) 3380, 3894, 7021, 7589
Eco32I GATATC 2 cut(s) 1765, 7624
Eco53kI GAGCTC 1 cut(s) 399
Eco72I CACGTG 1 cut(s) 4076
Eco81I CCTNAGG 1 cut(s) 7485
Eco88I CYCGRG 3 cut(s) 86, 1237, 7290
Eco91I GGTNACC 1 cut(s) 5779
EcoICRI GAGCTC 1 cut(s) 399
EcoNI CCTNNNNNAGG 2 cut(s) 6465, 7484
EcoO109I RGGNCCY 5 cut(s) 91, 3400, 5310, 5311, 5327
EcoO65I GGTNACC 1 cut(s) 5779
EcoRI GAATTC 7 cut(s) 658, 1327, 1533, 1999, 3208, 7321, 7598
EcoRII CCWGG 7 cut(s) 3291, 3348, 3617, 4546, 6451, 7903, 7926
EcoRV GATATC 2 cut(s) 1765, 7624
EcoT14I CCWWGG 9 cut(s) 762, 2182, 2249, 2635, 2991, 3189, 3411, 4172, 7976
EcoT22I ATGCAT 6 cut(s) 106, 1510, 2047, 4434, 6066, 7080
EcoT38I GRGCYC 5 cut(s) 291, 401, 3178, 5314, 5946
ErhI CCWWGG 9 cut(s) 762, 2182, 2249, 2635, 2991, 3189, 3411, 4172, 7976
Esp3I CGTCTC 1 cut(s) 798
FalI AAGNNNNNCTT 6 cut(s) 924, 956, 2563, 2595, 6138, 6170
FaqI GGGAC 5 cut(s) 748, 2201, 5042, 5902, 6805
FauI CCCGC 5 cut(s) 795, 3381, 4703, 5067, 6624
FauNDI CATATG 1 cut(s) 1315
FbaI TGATCA 3 cut(s) 2950, 5848, 6298
FblI GTMKAC 4 cut(s) 2878, 3055, 6118, 6210
FriOI GRGCYC 5 cut(s) 291, 401, 3178, 5314, 5946
FspI TGCGCA 2 cut(s) 3856, 5268
GlaI GCGC 6 cut(s) 1730, 2644, 3733, 3856, 5268, 6518
GsaI CCCAGC 4 cut(s) 220, 1326, 2338, 2852
GsuI CTGGAG 6 cut(s) 1318, 3080, 3389, 5449, 6413, 7584
HaeIII GGCC 8 cut(s) 1199, 2745, 3567, 3946, 4798, 5312, 5566, 6937
HapII CCGG 7 cut(s) 1200, 4739, 5563, 6947, 7317, 7443, 7581
HgaI GACGC 4 cut(s) 780, 2925, 4073, 6710
HhaI GCGC 6 cut(s) 1731, 2645, 3734, 3857, 5269, 6519
Hin1I GRCGYC 3 cut(s) 6472, 6508, 6721
Hin6I GCGC 6 cut(s) 1729, 2643, 3732, 3855, 5267, 6517
HinP1I GCGC 6 cut(s) 1729, 2643, 3732, 3855, 5267, 6517
HincII GTYRAC 3 cut(s) 1648, 2879, 7691
HindII GTYRAC 3 cut(s) 1648, 2879, 7691
HindIII AAGCTT 8 cut(s) 1284, 2054, 2202, 3860, 5228, 5586, 5655, 6900
HpaII CCGG 7 cut(s) 1200, 4739, 5563, 6947, 7317, 7443, 7581
Hpy99I CGWCG 7 cut(s) 287, 2880, 2883, 6167, 6170, 6173, 6176
HpyCH4IV ACGT 9 cut(s) 2454, 4038, 4075, 4162, 6261, 6472, 6508, 7825, 7957
HpySE526I ACGT 9 cut(s) 2454, 4038, 4075, 4162, 6261, 6472, 6508, 7825, 7957
Hsp92I GRCGYC 3 cut(s) 6472, 6508, 6721
HspAI GCGC 6 cut(s) 1729, 2643, 3732, 3855, 5267, 6517
KpnI GGTACC 2 cut(s) 515, 2870
KroI GCCGGC 1 cut(s) 1199
KroNI GCCGGC 1 cut(s) 1201
Ksp22I TGATCA 3 cut(s) 2950, 5848, 6298
LguI GCTCTTC 3 cut(s) 1713, 2517, 3167
MabI ACCWGGT 3 cut(s) 3291, 3617, 7903
MaeII ACGT 9 cut(s) 2454, 4038, 4075, 4162, 6261, 6472, 6508, 7825, 7957
MbiI CCGCTC 1 cut(s) 802
MfeI CAATTG 3 cut(s) 4068, 5148, 7967
MflI RGATCY 9 cut(s) 82, 2231, 2699, 4117, 5241, 5888, 6019, 6448, 7919
MhlI GDGCHC 8 cut(s) 291, 401, 627, 772, 808, 3178, 5314, 5946
MlyI GAGTC 9 cut(s) 1142, 2495, 3436, 5692, 5729, 5801, 5960, 6387, 7001
MmeI TCCRAC 7 cut(s) 935, 1037, 2089, 3631, 3844, 6571, 7598
Mph1103I ATGCAT 6 cut(s) 106, 1510, 2047, 4434, 6066, 7080
MroNI GCCGGC 1 cut(s) 1199
MroXI GAANNNNTTC 7 cut(s) 346, 662, 936, 2275, 2668, 5793, 7752
MslI CAYNNNNRTG 4 cut(s) 156, 375, 1350, 7230
MspCI CTTAAG 1 cut(s) 2051
MspI CCGG 7 cut(s) 1200, 4739, 5563, 6947, 7317, 7443, 7581
MspR9I CCNGG 8 cut(s) 3293, 3350, 3619, 4548, 6453, 7318, 7905, 7928
MunI CAATTG 3 cut(s) 4068, 5148, 7967
Mva1269I GAATGC 4 cut(s) 382, 4672, 6565, 7269
MvaI CCWGG 7 cut(s) 3293, 3350, 3619, 4548, 6453, 7905, 7928
MvnI CGCG 5 cut(s) 2643, 3376, 3732, 4824, 5963
NaeI GCCGGC 1 cut(s) 1201
NciI CCSGG 1 cut(s) 7318
NcoI CCATGG 2 cut(s) 3189, 3411
NdeI CATATG 1 cut(s) 1315
NgoMIV GCCGGC 1 cut(s) 1199
NheI GCTAGC 3 cut(s) 3266, 3337, 5453
NmeAIII GCCGAG 1 cut(s) 7490
NruI TCGCGA 1 cut(s) 4824
NsbI TGCGCA 2 cut(s) 3856, 5268
NsiI ATGCAT 6 cut(s) 106, 1510, 2047, 4434, 6066, 7080
NspV TTCGAA 2 cut(s) 2964, 7104
PaeI GCATGC 4 cut(s) 155, 2975, 4436, 7078
PaeR7I CTCGAG 2 cut(s) 1237, 7290
PagI TCATGA 3 cut(s) 796, 6301, 7225
PaqCI CACCTGC 2 cut(s) 4207, 6279
PceI AGGCCT 2 cut(s) 4798, 6937
PciI ACATGT 5 cut(s) 1559, 2009, 2398, 5791, 7065
PciSI GCTCTTC 3 cut(s) 1713, 2517, 3167
PcsI WCGNNNNNNNCGW 4 cut(s) 565, 6168, 6171, 7849
PctI GAATGC 4 cut(s) 382, 4672, 6565, 7269
PdiI GCCGGC 1 cut(s) 1201
PdmI GAANNNNTTC 7 cut(s) 346, 662, 936, 2275, 2668, 5793, 7752
PflFI GACNNNGTC 1 cut(s) 7709
PflMI CCANNNNNTGG 3 cut(s) 2608, 3412, 5716
PfoI TCCNGGA 1 cut(s) 7316
PinAI ACCGGT 1 cut(s) 7580
Ple19I CGATCG 2 cut(s) 4207, 4603
PleI GAGTC 9 cut(s) 1141, 2494, 3436, 5692, 5729, 5801, 5959, 6386, 7001
PmaCI CACGTG 1 cut(s) 4076
PmlI CACGTG 1 cut(s) 4076
PpsI GAGTC 9 cut(s) 1141, 2494, 3436, 5692, 5729, 5801, 5959, 6386, 7001
Ppu21I YACGTR 3 cut(s) 4076, 7826, 7958
PpuMI RGGWCCY 3 cut(s) 91, 3400, 5327
PscI ACATGT 5 cut(s) 1559, 2009, 2398, 5791, 7065
PshAI GACNNNNGTC 2 cut(s) 2637, 6598
PshBI ATTAAT 2 cut(s) 698, 6414
PsiI TTATAA 4 cut(s) 98, 437, 5958, 7848
Psp124BI GAGCTC 1 cut(s) 401
Psp5II RGGWCCY 3 cut(s) 91, 3400, 5327
Psp6I CCWGG 7 cut(s) 3291, 3348, 3617, 4546, 6451, 7903, 7926
PspCI CACGTG 1 cut(s) 4076
PspEI GGTNACC 1 cut(s) 5779
PspFI CCCAGC 4 cut(s) 216, 1322, 2334, 2848
PspGI CCWGG 7 cut(s) 3291, 3348, 3617, 4546, 6451, 7903, 7926
PspOMI GGGCCC 1 cut(s) 5310
PspPPI RGGWCCY 3 cut(s) 91, 3400, 5327
PspXI VCTCGAGB 1 cut(s) 7290
PstI CTGCAG 4 cut(s) 1432, 1924, 3870, 4446
PstNI CAGNNNCTG 3 cut(s) 1436, 2608, 5162
PsuI RGATCY 9 cut(s) 82, 2231, 2699, 4117, 5241, 5888, 6019, 6448, 7919
PsyI GACNNNGTC 1 cut(s) 7709
PvuI CGATCG 2 cut(s) 4207, 4603
PvuII CAGCTG 7 cut(s) 1115, 1258, 1919, 4661, 5939, 6896, 7121
RruI TCGCGA 1 cut(s) 4824
RseI CAYNNNNRTG 4 cut(s) 156, 375, 1350, 7230
Rsr2I CGGWCCG 1 cut(s) 714
RsrII CGGWCCG 1 cut(s) 714
SacI GAGCTC 1 cut(s) 401
SalI GTCGAC 1 cut(s) 2877
SapI GCTCTTC 3 cut(s) 1713, 2517, 3167
ScaI AGTACT 3 cut(s) 1846, 2116, 4015
SchI GAGTC 9 cut(s) 1142, 2495, 3436, 5692, 5729, 5801, 5960, 6387, 7001
ScrFI CCNGG 8 cut(s) 3293, 3350, 3619, 4548, 6453, 7318, 7905, 7928
SduI GDGCHC 8 cut(s) 291, 401, 627, 772, 808, 3178, 5314, 5946
SexAI ACCWGGT 3 cut(s) 3291, 3617, 7903
Sfr274I CTCGAG 2 cut(s) 1237, 7290
SfuI TTCGAA 2 cut(s) 2964, 7104
SgrDI CGTCGACG 1 cut(s) 2877
SlaI CTCGAG 2 cut(s) 1237, 7290
SmiMI CAYNNNNRTG 4 cut(s) 156, 375, 1350, 7230
SmlI CTYRAG 7 cut(s) 814, 1237, 1772, 2051, 5771, 6803, 7290
SmoI CTYRAG 7 cut(s) 814, 1237, 1772, 2051, 5771, 6803, 7290
SpeI ACTAGT 2 cut(s) 2648, 7801
SphI GCATGC 4 cut(s) 155, 2975, 4436, 7078
SseBI AGGCCT 2 cut(s) 4798, 6937
SspI AATATT 7 cut(s) 991, 3364, 4561, 5355, 5529, 5878, 6697
SstI GAGCTC 1 cut(s) 401
StuI AGGCCT 2 cut(s) 4798, 6937
StyD4I CCNGG 8 cut(s) 3291, 3348, 3617, 4546, 6451, 7316, 7903, 7926
StyI CCWWGG 9 cut(s) 762, 2182, 2249, 2635, 2991, 3189, 3411, 4172, 7976
TaiI ACGT 9 cut(s) 2457, 4041, 4078, 4165, 6264, 6475, 6511, 7828, 7960
TaqII GACCGA 1 cut(s) 6850
TatI WGTACW 9 cut(s) 386, 504, 591, 1260, 1844, 2077, 2114, 4013, 5048
TauI GCSGC 1 cut(s) 1322
TspGWI ACGGA 8 cut(s) 95, 557, 2369, 3212, 4568, 5373, 6692, 7078
Tth111I GACNNNGTC 1 cut(s) 7709
Van91I CCANNNNNTGG 3 cut(s) 2608, 3412, 5716
Vha464I CTTAAG 1 cut(s) 2051
VspI ATTAAT 2 cut(s) 698, 6414
XagI CCTNNNNNAGG 2 cut(s) 6465, 7484
XbaI TCTAGA 2 cut(s) 6503, 7898
XcmI CCANNNNNNNNNTGG 6 cut(s) 4136, 5927, 5975, 6023, 6767, 7154
XhoI CTCGAG 2 cut(s) 1237, 7290
XmaJI CCTAGG 1 cut(s) 2249
XmiI GTMKAC 4 cut(s) 2878, 3055, 6118, 6210
XmnI GAANNNNTTC 7 cut(s) 346, 662, 936, 2275, 2668, 5793, 7752
ZraI GACGTC 2 cut(s) 6473, 6509
ZrmI AGTACT 3 cut(s) 1846, 2116, 4015
Zsp2I ATGCAT 6 cut(s) 106, 1510, 2047, 4434, 6066, 7080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.