pycom08g17930

von Willebrand factor type A domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0014904
Physical Location & Seq
Reverse (-)
18121474 .. 18123586
2113 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g17930.1

Sequence Viewer

Length: 765 bp
ATGAATTGGAAGATCCCCCGGCCGGGACCAACACAATTTGTCTATCGAGTTGCGGGTTCCACGCTCTCTAGGAGTTCGTGGATCCCGACACGAAATGTCCGTCCGGTGTCTCTACAAGGATCCTGCAAATTGGTTGAGCACAATGACTACTCTAGTGCGCATCATTTGCTTTCATCGGCTCGGGCTCTGCTCATGAAATCCGGCTCAGTATCGGCTGACGAGTACGTGCGCGGCCTGGAAGCCGAGCTCTCAGAGCTGCATTGGAGAAGGCAGCACAAGATCATGGAGGAGCAGCAGCAGATGATGATGATGATCCAACGGCGGAGAGGTGGCGTGGAAAGGGACGTGGCGGGGACCGCTGTAGTGGATGAAAACAGGGAGCCGCTTACACCGAGTTCGGCTTGGAGAGCGGCTGAGAAGCTGGCTAAGGTGGCGATCATGTCGAAGAAGTCGCTGAATAGAGTGAGCGACTTGCACGGCTTTGAAAATAAAATGGTAGGAGCTTCTTGGCCTCATTACCCTCCTTGTGTCAGCGAAGGATGTACAAATGCTGCATGTTGTGGTCAAGAATTCAAATATGTTTGGCCTGAGCTAGTTGGAAAGCCTGCGTCGGAGGCCGCAGCGACAATCAACAAGAACAACCCAGCTGTTACAGTTCTCATCTTAAAACCTGGATTCATTAGATTGGACAACTTTTGCTGCAACCGTGTTTATGTTTTCACTGATGAAAAAGGCATTGTCTCGGAAGTACCTACAGTCGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA_2 zf-RING_11 zf-RING_2

Protein Analysis

255

Amino Acids

28.3

Weight (kDa)

9.08

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WAV3_C PF25243 40 - 94 3.4e-24 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
potato_inhibit PF00280 192 - 254 1.2e-20 Potato inhibitor I family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014904)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46860 AT3G50020 AT5G45277
fragaria_vesca FvH4_1g16190
malus_domestica MD15G1392900.v1.1
pyrus_communis pycom08g17930
rosa_chinensis RchiOBHm_Chr2g0105871 RchiOBHm_Chr6g0294471
rosa_roxburghii Rroxscaffold_2G00137770 Rroxscaffold_3G00227700 Rroxscaffold_5G00335710
rosa_rugosa Rorug02G0131200 Rorug02G0131400 Rorug07G0278000
rosa_samantha Rh2BG191900
rosa_wichuraiana Rw6G031180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 159
AccBSI CCGCTC 1 cut(s) 410
AccII CGCG 1 cut(s) 231
AciI CCGC 8 cut(s) 53, 231, 322, 350, 357, 383, 410, 618
AclWI GGATC 6 cut(s) 7, 76, 89, 114, 127, 307
AcoI YGGCCR 1 cut(s) 20
AcsI RAATTY 1 cut(s) 569
AfaI GTAC 3 cut(s) 224, 544, 750
AfiI CCNNNNNNNGG 3 cut(s) 22, 23, 758
AgsI TTSAA 2 cut(s) 485, 574
AjiI CACGTC 1 cut(s) 346
AjnI CCWGG 2 cut(s) 234, 670
AluBI AGCT 6 cut(s) 247, 256, 421, 503, 592, 647
AluI AGCT 6 cut(s) 247, 256, 421, 503, 592, 647
Alw21I GWGCWC 2 cut(s) 141, 249
Alw26I GTCTC 2 cut(s) 114, 745
AlwI GGATC 6 cut(s) 7, 76, 89, 114, 127, 307
Ama87I CYCGRG 1 cut(s) 180
AoxI GGCC 5 cut(s) 20, 232, 509, 584, 615
ApeKI GCWGC 7 cut(s) 256, 271, 292, 295, 551, 620, 699
ApoI RAATTY 1 cut(s) 569
ArsI GACNNNNNNTTYG 2 cut(s) 85, 117
AspLEI GCGC 2 cut(s) 160, 231
AspS9I GGNCC 2 cut(s) 26, 354
AsuC2I CCSGG 2 cut(s) 19, 24
AvaI CYCGRG 1 cut(s) 180
AvaII GGWCC 2 cut(s) 26, 354
BamHI GGATCC 2 cut(s) 81, 119
BanII GRGCYC 2 cut(s) 187, 249
Bbv12I GWGCWC 2 cut(s) 141, 249
BbvI GCAGC 7 cut(s) 243, 283, 304, 307, 538, 632, 686
BceAI ACGGC 2 cut(s) 335, 493
BciT130I CCWGG 2 cut(s) 236, 672
BcnI CCSGG 2 cut(s) 19, 24
BcoDI GTCTC 2 cut(s) 114, 745
BfaI CTAG 3 cut(s) 69, 153, 593
BfmI CTRYAG 2 cut(s) 360, 753
Bme1390I CCNGG 4 cut(s) 19, 24, 236, 672
Bme18I GGWCC 2 cut(s) 26, 354
BmeT110I CYCGRG 1 cut(s) 180
BmgBI CACGTC 1 cut(s) 346
BmgT120I GGNCC 2 cut(s) 26, 354
BmiI GGNNCC 6 cut(s) 27, 58, 83, 121, 355, 381
BmrFI CCNGG 4 cut(s) 19, 24, 236, 672
BmsI GCATC 1 cut(s) 169
Bpu10I CCTNAGC 2 cut(s) 426, 588
BpuMI CCSGG 2 cut(s) 19, 24
BsaAI YACGTR 1 cut(s) 226
BsaBI GATNNNNATC 1 cut(s) 311
BsaJI CCNNGG 1 cut(s) 17
BsaWI WCCGGW 1 cut(s) 103
Bsc4I CCNNNNNNNGG 3 cut(s) 22, 23, 758
Bse8I GATNNNNATC 1 cut(s) 311
BseBI CCWGG 2 cut(s) 236, 672
BseDI CCNNGG 1 cut(s) 17
BseGI GGATG 2 cut(s) 373, 545
BseJI GATNNNNATC 1 cut(s) 311
BseLI CCNNNNNNNGG 3 cut(s) 22, 23, 758
BseMII CTCAG 4 cut(s) 219, 264, 405, 579
BseRI GAGGAG 1 cut(s) 302
BseX3I CGGCCG 1 cut(s) 20
BseXI GCAGC 7 cut(s) 243, 283, 304, 307, 538, 632, 686
BseYI CCCAGC 1 cut(s) 643
Bsh1236I CGCG 1 cut(s) 231
Bsh1285I CGRYCG 1 cut(s) 23
BshFI GGCC 5 cut(s) 22, 234, 511, 586, 617
BsiEI CGRYCG 1 cut(s) 23
BsiHKAI GWGCWC 2 cut(s) 141, 249
BsiHKCI CYCGRG 1 cut(s) 180
BsiSI CCGG 4 cut(s) 19, 23, 104, 201
BslFI GGGAC 3 cut(s) 39, 356, 367
BslI CCNNNNNNNGG 3 cut(s) 22, 23, 758
BsmAI GTCTC 2 cut(s) 114, 745
BsmFI GGGAC 3 cut(s) 39, 356, 367
BsnI GGCC 5 cut(s) 22, 234, 511, 586, 617
BsoBI CYCGRG 1 cut(s) 180
Bsp1286I GDGCHC 3 cut(s) 141, 187, 249
Bsp1407I TGTACA 1 cut(s) 542
Bsp143I GATC 6 cut(s) 12, 81, 119, 279, 312, 435
BspACI CCGC 8 cut(s) 53, 231, 322, 350, 357, 383, 410, 618
BspANI GGCC 5 cut(s) 22, 234, 511, 586, 617
BspCNI CTCAG 4 cut(s) 218, 263, 406, 580
BspFNI CGCG 1 cut(s) 231
BspHI TCATGA 1 cut(s) 192
BspLI GGNNCC 6 cut(s) 27, 58, 83, 121, 355, 381
BspPI GGATC 6 cut(s) 7, 76, 89, 114, 127, 307
BsrBI CCGCTC 1 cut(s) 410
BsrGI TGTACA 1 cut(s) 542
BssECI CCNNGG 1 cut(s) 17
BssMI GATC 6 cut(s) 12, 81, 119, 279, 312, 435
Bst2UI CCWGG 2 cut(s) 236, 672
Bst4CI ACNGT 3 cut(s) 655, 707, 757
BstAPI GCANNNNNTGC 1 cut(s) 166
BstAUI TGTACA 1 cut(s) 542
BstBAI YACGTR 1 cut(s) 226
BstC8I GCNNGC 2 cut(s) 423, 606
BstDEI CTNAG 5 cut(s) 205, 250, 414, 426, 588
BstF5I GGATG 2 cut(s) 373, 545
BstFNI CGCG 1 cut(s) 231
BstHHI GCGC 2 cut(s) 160, 231
BstKTI GATC 6 cut(s) 15, 84, 122, 282, 315, 438
BstMAI GTCTC 2 cut(s) 114, 745
BstMBI GATC 6 cut(s) 12, 81, 119, 279, 312, 435
BstMCI CGRYCG 1 cut(s) 23
BstMWI GCNNNNNNNGC 6 cut(s) 166, 253, 356, 407, 431, 614
BstNI CCWGG 2 cut(s) 236, 672
BstNSI RCATGY 1 cut(s) 558
BstSCI CCNGG 4 cut(s) 17, 22, 234, 670
BstSFI CTRYAG 2 cut(s) 360, 753
BstUI CGCG 1 cut(s) 231
BstV1I GCAGC 7 cut(s) 243, 283, 304, 307, 538, 632, 686
BstX2I RGATCY 3 cut(s) 12, 81, 119
BstYI RGATCY 3 cut(s) 12, 81, 119
BstZI CGGCCG 1 cut(s) 20
BsuRI GGCC 5 cut(s) 22, 234, 511, 586, 617
BtrI CACGTC 1 cut(s) 346
BtsCI GGATG 2 cut(s) 373, 545
BtsIMutI CAGTG 1 cut(s) 720
Cac8I GCNNGC 2 cut(s) 423, 606
CciI TCATGA 1 cut(s) 192
CfoI GCGC 2 cut(s) 160, 231
Cfr13I GGNCC 2 cut(s) 26, 354
CseI GACGC 1 cut(s) 597
Csp6I GTAC 3 cut(s) 223, 543, 749
CviAII CATG 4 cut(s) 193, 283, 439, 555
CviQI GTAC 3 cut(s) 223, 543, 749
DdeI CTNAG 5 cut(s) 205, 250, 414, 426, 588
DpnI GATC 6 cut(s) 14, 83, 121, 281, 314, 437
DpnII GATC 6 cut(s) 12, 81, 119, 279, 312, 435
EaeI YGGCCR 1 cut(s) 20
EagI CGGCCG 1 cut(s) 20
EciI GGCGGA 1 cut(s) 337
Ecl136II GAGCTC 1 cut(s) 247
EclXI CGGCCG 1 cut(s) 20
Eco24I GRGCYC 2 cut(s) 187, 249
Eco47I GGWCC 2 cut(s) 26, 354
Eco52I CGGCCG 1 cut(s) 20
Eco53kI GAGCTC 1 cut(s) 247
Eco88I CYCGRG 1 cut(s) 180
EcoICRI GAGCTC 1 cut(s) 247
EcoRI GAATTC 1 cut(s) 569
EcoRII CCWGG 2 cut(s) 234, 670
EcoT38I GRGCYC 2 cut(s) 187, 249
FaeI CATG 4 cut(s) 196, 286, 442, 558
FaiI YATR 6 cut(s) 194, 284, 440, 556, 579, 714
FaqI GGGAC 3 cut(s) 39, 356, 367
FatI CATG 4 cut(s) 192, 282, 438, 554
FauI CCCGC 2 cut(s) 46, 343
FokI GGATG 2 cut(s) 380, 552
FriOI GRGCYC 2 cut(s) 187, 249
FspAI RTGCGCAY 1 cut(s) 159
FspBI CTAG 3 cut(s) 69, 153, 593
FspI TGCGCA 1 cut(s) 159
GlaI GCGC 2 cut(s) 159, 230
GsaI CCCAGC 1 cut(s) 647
HaeIII GGCC 5 cut(s) 22, 234, 511, 586, 617
HapII CCGG 4 cut(s) 19, 23, 104, 201
HgaI GACGC 1 cut(s) 597
HhaI GCGC 2 cut(s) 160, 231
Hin1II CATG 4 cut(s) 196, 286, 442, 558
Hin6I GCGC 2 cut(s) 158, 229
HinP1I GCGC 2 cut(s) 158, 229
HinfI GANTC 1 cut(s) 675
HpaII CCGG 4 cut(s) 19, 23, 104, 201
Hpy188I TCNGA 4 cut(s) 253, 613, 745, 761
Hpy188III TCNNGA 3 cut(s) 85, 193, 566
Hpy99I CGWCG 1 cut(s) 613
HpyAV CCTTC 2 cut(s) 261, 530
HpyCH4III ACNGT 3 cut(s) 655, 707, 757
HpyCH4IV ACGT 2 cut(s) 225, 345
HpyCH4V TGCA 5 cut(s) 126, 259, 475, 554, 702
HpyF10VI GCNNNNNNNGC 6 cut(s) 166, 253, 356, 407, 431, 614
HpyF3I CTNAG 5 cut(s) 205, 250, 414, 426, 588
HpySE526I ACGT 2 cut(s) 225, 345
Hsp92II CATG 4 cut(s) 196, 286, 442, 558
HspAI GCGC 2 cut(s) 158, 229
Kzo9I GATC 6 cut(s) 12, 81, 119, 279, 312, 435
LmnI GCTCC 3 cut(s) 289, 379, 500
Lsp1109I GCAGC 7 cut(s) 243, 283, 304, 307, 538, 632, 686
LweI GCATC 1 cut(s) 169
MaeI CTAG 3 cut(s) 69, 153, 593
MaeII ACGT 2 cut(s) 225, 345
MaeIII GTNAC 1 cut(s) 649
MalI GATC 6 cut(s) 14, 83, 121, 281, 314, 437
MbiI CCGCTC 1 cut(s) 410
MboI GATC 6 cut(s) 12, 81, 119, 279, 312, 435
MboII GAAGA 2 cut(s) 22, 457
MflI RGATCY 3 cut(s) 12, 81, 119
MhlI GDGCHC 3 cut(s) 141, 187, 249
MluCI AATT 4 cut(s) 4, 35, 128, 569
MmeI TCCRAC 4 cut(s) 340, 577, 591, 739
MnlI CCTC 5 cut(s) 280, 320, 522, 531, 607
MseI TTAA 1 cut(s) 665
MspA1I CMGCKG 2 cut(s) 359, 647
MspI CCGG 4 cut(s) 19, 23, 104, 201
MspR9I CCNGG 4 cut(s) 19, 24, 236, 672
MvaI CCWGG 2 cut(s) 236, 672
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 6 cut(s) 166, 253, 356, 407, 431, 614
NciI CCSGG 2 cut(s) 19, 24
NdeII GATC 6 cut(s) 12, 81, 119, 279, 312, 435
NlaIII CATG 4 cut(s) 196, 286, 442, 558
NlaIV GGNNCC 6 cut(s) 27, 58, 83, 121, 355, 381
NmeAIII GCCGAG 1 cut(s) 268
NsbI TGCGCA 1 cut(s) 159
NspI RCATGY 1 cut(s) 558
PagI TCATGA 1 cut(s) 192
PcsI WCGNNNNNNNCGW 2 cut(s) 83, 97
PfeI GAWTC 1 cut(s) 675
Ppu21I YACGTR 1 cut(s) 226
Psp124BI GAGCTC 1 cut(s) 249
Psp6I CCWGG 2 cut(s) 234, 670
PspFI CCCAGC 1 cut(s) 643
PspGI CCWGG 2 cut(s) 234, 670
PspN4I GGNNCC 6 cut(s) 27, 58, 83, 121, 355, 381
PspPI GGNCC 2 cut(s) 26, 354
PsuI RGATCY 3 cut(s) 12, 81, 119
PvuII CAGCTG 1 cut(s) 647
RsaI GTAC 3 cut(s) 224, 544, 750
RsaNI GTAC 3 cut(s) 223, 543, 749
SacI GAGCTC 1 cut(s) 249
SaqAI TTAA 1 cut(s) 665
Sau3AI GATC 6 cut(s) 12, 81, 119, 279, 312, 435
Sau96I GGNCC 2 cut(s) 26, 354
ScrFI CCNGG 4 cut(s) 19, 24, 236, 672
SduI GDGCHC 3 cut(s) 141, 187, 249
SfaNI GCATC 1 cut(s) 169
SfcI CTRYAG 2 cut(s) 360, 753
SinI GGWCC 2 cut(s) 26, 354
Sse9I AATT 4 cut(s) 4, 35, 128, 569
SsiI CCGC 8 cut(s) 53, 231, 322, 350, 357, 383, 410, 618
SspMI CTAG 3 cut(s) 69, 153, 593
SstI GAGCTC 1 cut(s) 249
StyD4I CCNGG 4 cut(s) 17, 22, 234, 670
TaaI ACNGT 3 cut(s) 655, 707, 757
TaiI ACGT 2 cut(s) 228, 348
TaqI TCGA 2 cut(s) 46, 443
TasI AATT 4 cut(s) 4, 35, 128, 569
TatI WGTACW 1 cut(s) 542
TauI GCSGC 4 cut(s) 234, 385, 413, 620
TfiI GAWTC 1 cut(s) 675
Tru1I TTAA 1 cut(s) 665
Tru9I TTAA 1 cut(s) 665
TscAI CASTG 1 cut(s) 727
TseI GCWGC 7 cut(s) 256, 271, 292, 295, 551, 620, 699
TspDTI ATGAA 6 cut(s) 17, 162, 209, 384, 667, 741
TspGWI ACGGA 1 cut(s) 89
TspRI CASTG 1 cut(s) 727
VpaK11BI GGWCC 2 cut(s) 26, 354
XapI RAATTY 1 cut(s) 569
XceI RCATGY 1 cut(s) 558
XspI CTAG 3 cut(s) 69, 153, 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.