Rh2BG191900

Potato inhibitor I family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0014904
Physical Location & Seq
Forward (+)
17270407 .. 17311881
41475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG191900.1

Sequence Viewer

Length: 738 bp
ATGAAAGTAAACTTAGAAGCATGGTTTTTATCATTGCAGGCTCTTCTTCACAATGGATCAAGGCCACCACCATTTGATAATGTTAGTAATCTGTGTATGCATATTGCTAACTTTGTTGCTGAACTGGTGCCGGCAATAGTCTCTTTATTCAGAGGAATGCTTCAGTTGTGTTCTTTACGTTTCGGTAATTTATACCTGAAATCTAAGGAATCTATACAGGACAATGCTCGGCCAATTGTTTGTGTCTGGCGAGTTAGCAAAGTAGCACGGCCGAATCTTCGGCCACTCGACCAAGGATACAAGCATATATGGCCTGAACTACTTGGGAAGTCTGGGCAGGAAGCCAAAGCGATAATTAAGAAAGACAACCCCTTGGTTACCACTGTGATGGTAAAACCAGGCATGGCGGTAACTAATGATTATTGCTGCAACCGTGTATGGATCAGGGTGGATGACCATGGCAATGTCTACCTTGCACCAAACATAGGTTCAGATCCCTATTGCGGCGGTTGGTCAGAAGCAAAAGGCTGGTACGGCGCGGCTCTTCTGGTAATGGATGCACATGGACATATATGTACTGAACGAGCACTTAACAAGCGCGGGCAGGAAGCTATAGAGGGTCCAGAGTATAGGCGGTCTTCACCATTGCGTTCCGTACAAGTTGATGCCTCAGTGAAAGACAAAATGACATGGGTCAGCGAAGTGCGAGTGCATTTTGCGAACTTTGGAGTCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
potato_inhibit

Protein Analysis

245

Amino Acids

27.44

Weight (kDa)

9.07

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
potato_inhibit PF00280 101 - 163 7.2e-23 Potato inhibitor I family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014904)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46860 AT3G50020 AT5G45277
fragaria_vesca FvH4_1g16190
malus_domestica MD15G1392900.v1.1
pyrus_communis pycom08g17930
rosa_chinensis RchiOBHm_Chr2g0105871 RchiOBHm_Chr6g0294471
rosa_roxburghii Rroxscaffold_2G00137770 Rroxscaffold_3G00227700 Rroxscaffold_5G00335710
rosa_rugosa Rorug02G0131200 Rorug02G0131400 Rorug07G0278000
rosa_samantha Rh2BG191900
rosa_wichuraiana Rw6G031180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 127
AccI GTMKAC 1 cut(s) 468
AccII CGCG 2 cut(s) 539, 600
AciI CCGC 6 cut(s) 407, 504, 507, 539, 600, 634
AclWI GGATC 3 cut(s) 64, 449, 488
AcoI YGGCCR 3 cut(s) 230, 269, 281
AcuI CTGAAG 1 cut(s) 146
AfaI GTAC 3 cut(s) 533, 577, 657
AfiI CCNNNNNNNGG 2 cut(s) 485, 503
AjnI CCWGG 1 cut(s) 397
AjuI GAANNNNNNNTTGG 2 cut(s) 472, 504
AluBI AGCT 1 cut(s) 611
AluI AGCT 1 cut(s) 611
Alw21I GWGCWC 1 cut(s) 589
Alw26I GTCTC 1 cut(s) 145
AlwI GGATC 3 cut(s) 64, 449, 488
AoxI GGCC 5 cut(s) 62, 230, 269, 281, 311
ApeKI GCWGC 1 cut(s) 426
AspLEI GCGC 2 cut(s) 539, 600
AspS9I GGNCC 1 cut(s) 620
AsuHPI GGTGA 1 cut(s) 633
AvaII GGWCC 1 cut(s) 620
BanI GGYRCC 1 cut(s) 127
BbsI GAAGAC 1 cut(s) 630
Bbv12I GWGCWC 1 cut(s) 589
BbvI GCAGC 1 cut(s) 413
BccI CCATC 1 cut(s) 382
BceAI ACGGC 2 cut(s) 284, 550
BciT130I CCWGG 1 cut(s) 399
BciVI GTATCC 1 cut(s) 290
BcoDI GTCTC 1 cut(s) 145
BfmI CTRYAG 1 cut(s) 612
BfuI GTATCC 1 cut(s) 290
BisI GCNGC 3 cut(s) 427, 505, 540
BlsI GCNGC 3 cut(s) 428, 506, 541
Bme1390I CCNGG 1 cut(s) 399
Bme18I GGWCC 1 cut(s) 620
BmgT120I GGNCC 1 cut(s) 620
BmiI GGNNCC 2 cut(s) 129, 621
BmrFI CCNGG 1 cut(s) 399
BmsI GCATC 2 cut(s) 547, 655
BoxI GACNNNNGTC 1 cut(s) 692
BpiI GAAGAC 1 cut(s) 630
BsaJI CCNNGG 3 cut(s) 292, 372, 457
Bsc4I CCNNNNNNNGG 2 cut(s) 485, 503
Bse118I RCCGGY 1 cut(s) 130
Bse1I ACTGG 1 cut(s) 129
Bse3DI GCAATG 3 cut(s) 32, 469, 644
BseBI CCWGG 1 cut(s) 399
BseDI CCNNGG 3 cut(s) 292, 372, 457
BseGI GGATG 2 cut(s) 457, 562
BseLI CCNNNNNNNGG 2 cut(s) 485, 503
BseMI GCAATG 3 cut(s) 32, 469, 644
BseMII CTCAG 1 cut(s) 684
BseNI ACTGG 1 cut(s) 129
BseX3I CGGCCG 1 cut(s) 269
BseXI GCAGC 1 cut(s) 413
Bsh1236I CGCG 2 cut(s) 539, 600
Bsh1285I CGRYCG 1 cut(s) 272
BshFI GGCC 5 cut(s) 64, 232, 271, 283, 313
BshNI GGYRCC 1 cut(s) 127
BsiEI CGRYCG 1 cut(s) 272
BsiHKAI GWGCWC 1 cut(s) 589
BsiSI CCGG 1 cut(s) 131
BslI CCNNNNNNNGG 2 cut(s) 485, 503
BsmAI GTCTC 1 cut(s) 145
BsmI GAATGC 1 cut(s) 162
BsnI GGCC 5 cut(s) 64, 232, 271, 283, 313
Bsp1286I GDGCHC 1 cut(s) 589
Bsp143I GATC 3 cut(s) 56, 441, 493
Bsp19I CCATGG 1 cut(s) 457
BspACI CCGC 6 cut(s) 407, 504, 507, 539, 600, 634
BspANI GGCC 5 cut(s) 64, 232, 271, 283, 313
BspCNI CTCAG 1 cut(s) 683
BspFNI CGCG 2 cut(s) 539, 600
BspLI GGNNCC 2 cut(s) 129, 621
BspPI GGATC 3 cut(s) 64, 449, 488
BspQI GCTCTTC 2 cut(s) 48, 549
BspT107I GGYRCC 1 cut(s) 127
BsrDI GCAATG 3 cut(s) 32, 469, 644
BsrFI RCCGGY 1 cut(s) 130
BsrI ACTGG 1 cut(s) 129
BssAI RCCGGY 1 cut(s) 130
BssECI CCNNGG 3 cut(s) 292, 372, 457
BssMI GATC 3 cut(s) 56, 441, 493
BssT1I CCWWGG 3 cut(s) 292, 372, 457
Bst2UI CCWGG 1 cut(s) 399
Bst4CI ACNGT 2 cut(s) 385, 434
Bst6I CTCTTC 2 cut(s) 48, 549
BstC8I GCNNGC 3 cut(s) 39, 132, 602
BstDEI CTNAG 3 cut(s) 13, 204, 670
BstDSI CCRYGG 1 cut(s) 457
BstEII GGTNACC 1 cut(s) 376
BstF5I GGATG 2 cut(s) 457, 562
BstFNI CGCG 2 cut(s) 539, 600
BstHHI GCGC 2 cut(s) 539, 600
BstKTI GATC 3 cut(s) 59, 444, 496
BstMAI GTCTC 1 cut(s) 145
BstMBI GATC 3 cut(s) 56, 441, 493
BstMCI CGRYCG 1 cut(s) 272
BstMWI GCNNNNNNNGC 2 cut(s) 310, 534
BstNI CCWGG 1 cut(s) 399
BstPAI GACNNNNGTC 1 cut(s) 692
BstPI GGTNACC 1 cut(s) 376
BstSCI CCNGG 1 cut(s) 397
BstSFI CTRYAG 1 cut(s) 612
BstUI CGCG 2 cut(s) 539, 600
BstV1I GCAGC 1 cut(s) 413
BstV2I GAAGAC 1 cut(s) 630
BstX2I RGATCY 1 cut(s) 493
BstXI CCANNNNNNTGG 1 cut(s) 388
BstYI RGATCY 1 cut(s) 493
BstZI CGGCCG 1 cut(s) 269
BsuI GTATCC 1 cut(s) 290
BsuRI GGCC 5 cut(s) 64, 232, 271, 283, 313
BtgI CCRYGG 1 cut(s) 457
BtsCI GGATG 2 cut(s) 457, 562
BtsIMutI CAGTG 2 cut(s) 381, 678
Cac8I GCNNGC 3 cut(s) 39, 132, 602
CfoI GCGC 2 cut(s) 539, 600
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 1 cut(s) 620
Csp6I GTAC 3 cut(s) 532, 576, 656
CviAII CATG 5 cut(s) 21, 403, 458, 563, 690
CviQI GTAC 3 cut(s) 532, 576, 656
DdeI CTNAG 3 cut(s) 13, 204, 670
DpnI GATC 3 cut(s) 58, 443, 495
DpnII GATC 3 cut(s) 56, 441, 493
EaeI YGGCCR 3 cut(s) 230, 269, 281
EagI CGGCCG 1 cut(s) 269
Eam1104I CTCTTC 2 cut(s) 48, 549
EarI CTCTTC 2 cut(s) 48, 549
EclXI CGGCCG 1 cut(s) 269
Eco130I CCWWGG 3 cut(s) 292, 372, 457
Eco47I GGWCC 1 cut(s) 620
Eco52I CGGCCG 1 cut(s) 269
Eco57I CTGAAG 1 cut(s) 146
Eco91I GGTNACC 1 cut(s) 376
EcoO65I GGTNACC 1 cut(s) 376
EcoRII CCWGG 1 cut(s) 397
EcoT14I CCWWGG 3 cut(s) 292, 372, 457
EcoT22I ATGCAT 1 cut(s) 102
ErhI CCWWGG 3 cut(s) 292, 372, 457
FaeI CATG 5 cut(s) 24, 406, 461, 566, 693
FatI CATG 5 cut(s) 20, 402, 457, 562, 689
FauI CCCGC 1 cut(s) 593
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 3 cut(s) 427, 505, 540
FokI GGATG 2 cut(s) 464, 569
Fsp4HI GCNGC 3 cut(s) 427, 505, 540
GlaI GCGC 2 cut(s) 538, 599
GluI GCNGC 3 cut(s) 427, 505, 540
HaeIII GGCC 5 cut(s) 64, 232, 271, 283, 313
HapII CCGG 1 cut(s) 131
HhaI GCGC 2 cut(s) 539, 600
Hin1II CATG 5 cut(s) 24, 406, 461, 566, 693
Hin6I GCGC 2 cut(s) 537, 598
HinP1I GCGC 2 cut(s) 537, 598
HinfI GANTC 3 cut(s) 209, 274, 729
HpaII CCGG 1 cut(s) 131
HphI GGTGA 1 cut(s) 633
Hpy166II GTNNAC 2 cut(s) 10, 469
Hpy188I TCNGA 3 cut(s) 152, 493, 517
Hpy188III TCNNGA 1 cut(s) 623
Hpy8I GTNNAC 2 cut(s) 10, 469
HpyCH4III ACNGT 2 cut(s) 385, 434
HpyCH4IV ACGT 1 cut(s) 178
HpyCH4V TGCA 6 cut(s) 37, 100, 429, 476, 560, 712
HpyF10VI GCNNNNNNNGC 2 cut(s) 310, 534
HpyF3I CTNAG 3 cut(s) 13, 204, 670
HpySE526I ACGT 1 cut(s) 178
Hsp92II CATG 5 cut(s) 24, 406, 461, 566, 693
HspAI GCGC 2 cut(s) 537, 598
KroI GCCGGC 1 cut(s) 130
KroNI GCCGGC 1 cut(s) 132
Kzo9I GATC 3 cut(s) 56, 441, 493
LguI GCTCTTC 2 cut(s) 48, 549
Lsp1109I GCAGC 1 cut(s) 413
LweI GCATC 2 cut(s) 547, 655
MaeII ACGT 1 cut(s) 178
MaeIII GTNAC 2 cut(s) 376, 409
MalI GATC 3 cut(s) 58, 443, 495
MboI GATC 3 cut(s) 56, 441, 493
MboII GAAGA 5 cut(s) 35, 38, 269, 536, 630
MfeI CAATTG 1 cut(s) 234
MflI RGATCY 1 cut(s) 493
MhlI GDGCHC 1 cut(s) 589
MluCI AATT 3 cut(s) 187, 234, 354
MlyI GAGTC 1 cut(s) 738
MnlI CCTC 3 cut(s) 146, 610, 679
Mph1103I ATGCAT 1 cut(s) 102
MroNI GCCGGC 1 cut(s) 130
MseI TTAA 2 cut(s) 357, 591
MslI CAYNNNNRTG 2 cut(s) 386, 462
MspI CCGG 1 cut(s) 131
MspR9I CCNGG 1 cut(s) 399
MunI CAATTG 1 cut(s) 234
Mva1269I GAATGC 1 cut(s) 162
MvaI CCWGG 1 cut(s) 399
MvnI CGCG 2 cut(s) 539, 600
MwoI GCNNNNNNNGC 2 cut(s) 310, 534
NaeI GCCGGC 1 cut(s) 132
NcoI CCATGG 1 cut(s) 457
NdeII GATC 3 cut(s) 56, 441, 493
NgoMIV GCCGGC 1 cut(s) 130
NlaIII CATG 5 cut(s) 24, 406, 461, 566, 693
NlaIV GGNNCC 2 cut(s) 129, 621
NmeAIII GCCGAG 1 cut(s) 208
NsiI ATGCAT 1 cut(s) 102
PciSI GCTCTTC 2 cut(s) 48, 549
PctI GAATGC 1 cut(s) 162
PdiI GCCGGC 1 cut(s) 132
PfeI GAWTC 2 cut(s) 209, 274
PkrI GCNGC 3 cut(s) 428, 506, 541
PleI GAGTC 1 cut(s) 737
PpsI GAGTC 1 cut(s) 737
PshAI GACNNNNGTC 1 cut(s) 692
Psp6I CCWGG 1 cut(s) 397
PspEI GGTNACC 1 cut(s) 376
PspGI CCWGG 1 cut(s) 397
PspN4I GGNNCC 2 cut(s) 129, 621
PspPI GGNCC 1 cut(s) 620
PsuI RGATCY 1 cut(s) 493
RsaI GTAC 3 cut(s) 533, 577, 657
RsaNI GTAC 3 cut(s) 532, 576, 656
RseI CAYNNNNRTG 2 cut(s) 386, 462
SapI GCTCTTC 2 cut(s) 48, 549
SaqAI TTAA 2 cut(s) 357, 591
SatI GCNGC 3 cut(s) 427, 505, 540
Sau3AI GATC 3 cut(s) 56, 441, 493
Sau96I GGNCC 1 cut(s) 620
SchI GAGTC 1 cut(s) 738
ScrFI CCNGG 1 cut(s) 399
SduI GDGCHC 1 cut(s) 589
SetI ASST 5 cut(s) 181, 198, 474, 490, 613
SfaNI GCATC 2 cut(s) 547, 655
SfcI CTRYAG 1 cut(s) 612
SinI GGWCC 1 cut(s) 620
SmiMI CAYNNNNRTG 2 cut(s) 386, 462
Sse9I AATT 3 cut(s) 187, 234, 354
SsiI CCGC 6 cut(s) 407, 504, 507, 539, 600, 634
StyD4I CCNGG 1 cut(s) 397
StyI CCWWGG 3 cut(s) 292, 372, 457
TaaI ACNGT 2 cut(s) 385, 434
TaiI ACGT 1 cut(s) 181
TaqI TCGA 1 cut(s) 288
TasI AATT 3 cut(s) 187, 234, 354
TatI WGTACW 1 cut(s) 575
TauI GCSGC 2 cut(s) 507, 542
TfiI GAWTC 2 cut(s) 209, 274
Tru1I TTAA 2 cut(s) 357, 591
Tru9I TTAA 2 cut(s) 357, 591
TscAI CASTG 2 cut(s) 388, 678
TseI GCWGC 1 cut(s) 426
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 643
TspRI CASTG 2 cut(s) 388, 678
VpaK11BI GGWCC 1 cut(s) 620
XmiI GTMKAC 1 cut(s) 468
Zsp2I ATGCAT 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.