pycom08g19090

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008981
Physical Location & Seq
Forward (+)
19142407 .. 19142854
448 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 324 bp
ATGGATTCGGTTAGAGGACAAGGAGGCATCCAGATGTTGCTAACAGCTGAACAGGATGCTCAACAAATTGTGAACAATGCTAGAAACTCGAAGATGGCACGCTTGAGACAAGCTAAAGAAGAAGCTGATAGGGAAGTGCAACTCTATCGCACGAATATGGAGGCTGAGCACCAAAAGAAAATTGCAGATGTACTAACTAGTCTTTCATCAACTACCCAAATGTATCAGCGTCACTCCATTGTTCACCAAAAAAAAAAAAATGATCTTTTTTGTGATGAAATAATTAGCAGCATTATGATTCATACCAACATAACACACGCATAA

Protein Analysis

108

Amino Acids

12.27

Weight (kDa)

7.94

Isoelectric Point (pI)

33.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-ATPase_G PF03179 7 - 84 9e-20 Vacuolar (H+)-ATPase G subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 192
AhlI ACTAGT 1 cut(s) 197
AluBI AGCT 3 cut(s) 47, 113, 125
AluI AGCT 3 cut(s) 47, 113, 125
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 1 cut(s) 100
ApeKI GCWGC 1 cut(s) 288
AsuHPI GGTGA 1 cut(s) 236
Bbv12I GWGCWC 1 cut(s) 171
BbvI GCAGC 1 cut(s) 300
BccI CCATC 1 cut(s) 88
BcgI CGANNNNNNTGC 2 cut(s) 128, 162
BcoDI GTCTC 1 cut(s) 100
BcuI ACTAGT 1 cut(s) 197
BfaI CTAG 2 cut(s) 81, 198
BisI GCNGC 1 cut(s) 289
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 1 cut(s) 290
BmsI GCATC 2 cut(s) 36, 46
Bpu1102I GCTNAGC 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 124
BseGI GGATG 2 cut(s) 27, 61
BseMII CTCAG 1 cut(s) 156
BseXI GCAGC 1 cut(s) 300
BsiHKAI GWGCWC 1 cut(s) 171
BsmAI GTCTC 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 171
Bsp143I GATC 1 cut(s) 262
Bsp1720I GCTNAGC 1 cut(s) 165
BspCNI CTCAG 1 cut(s) 157
BssMI GATC 1 cut(s) 262
BstC8I GCNNGC 1 cut(s) 100
BstDEI CTNAG 1 cut(s) 165
BstF5I GGATG 2 cut(s) 27, 61
BstKTI GATC 1 cut(s) 265
BstMAI GTCTC 1 cut(s) 100
BstMBI GATC 1 cut(s) 262
BstV1I GCAGC 1 cut(s) 300
BtsCI GGATG 2 cut(s) 27, 61
Cac8I GCNNGC 1 cut(s) 100
CseI GACGC 1 cut(s) 218
Csp6I GTAC 1 cut(s) 191
CviJI RGCY 4 cut(s) 47, 113, 125, 164
CviKI_1 RGCY 4 cut(s) 47, 113, 125, 164
CviQI GTAC 1 cut(s) 191
DdeI CTNAG 1 cut(s) 165
DpnI GATC 1 cut(s) 264
DpnII GATC 1 cut(s) 262
FaiI YATR 5 cut(s) 158, 296, 303, 311, 322
Fnu4HI GCNGC 1 cut(s) 289
FokI GGATG 2 cut(s) 14, 68
Fsp4HI GCNGC 1 cut(s) 289
FspBI CTAG 2 cut(s) 81, 198
GluI GCNGC 1 cut(s) 289
HgaI GACGC 1 cut(s) 218
HinfI GANTC 2 cut(s) 5, 298
HphI GGTGA 1 cut(s) 236
Hpy166II GTNNAC 2 cut(s) 73, 244
Hpy188III TCNNGA 1 cut(s) 31
Hpy8I GTNNAC 2 cut(s) 73, 244
HpyCH4V TGCA 2 cut(s) 139, 185
HpyF3I CTNAG 1 cut(s) 165
Kzo9I GATC 1 cut(s) 262
LpnPI CCDG 2 cut(s) 38, 44
Lsp1109I GCAGC 1 cut(s) 300
LweI GCATC 2 cut(s) 36, 46
MaeI CTAG 2 cut(s) 81, 198
MaeIII GTNAC 1 cut(s) 230
MalI GATC 1 cut(s) 264
MboI GATC 1 cut(s) 262
MboII GAAGA 2 cut(s) 103, 131
MhlI GDGCHC 1 cut(s) 171
MluCI AATT 3 cut(s) 66, 180, 282
MnlI CCTC 3 cut(s) 8, 17, 154
MslI CAYNNNNRTG 2 cut(s) 32, 155
MspA1I CMGCKG 1 cut(s) 47
NdeII GATC 1 cut(s) 262
NmuCI GTSAC 1 cut(s) 230
PfeI GAWTC 2 cut(s) 5, 298
PkrI GCNGC 1 cut(s) 290
PvuII CAGCTG 1 cut(s) 47
RsaI GTAC 1 cut(s) 192
RsaNI GTAC 1 cut(s) 191
RseI CAYNNNNRTG 2 cut(s) 32, 155
SatI GCNGC 1 cut(s) 289
Sau3AI GATC 1 cut(s) 262
SduI GDGCHC 1 cut(s) 171
SetI ASST 3 cut(s) 49, 115, 127
SfaNI GCATC 2 cut(s) 36, 46
SmiMI CAYNNNNRTG 2 cut(s) 32, 155
SmlI CTYRAG 1 cut(s) 103
SmoI CTYRAG 1 cut(s) 103
SpeI ACTAGT 1 cut(s) 197
Sse9I AATT 3 cut(s) 66, 180, 282
SspMI CTAG 2 cut(s) 81, 198
TaqI TCGA 1 cut(s) 89
TasI AATT 3 cut(s) 66, 180, 282
TatI WGTACW 1 cut(s) 190
TfiI GAWTC 2 cut(s) 5, 298
TseFI GTSAC 1 cut(s) 230
TseI GCWGC 1 cut(s) 288
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 3 cut(s) 195, 290, 291
XspI CTAG 2 cut(s) 81, 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.