pycom09g00140

DSBA-like thioredoxin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0005301
Physical Location & Seq
Forward (+)
110373 .. 112431
2059 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 777 bp
ATGCCACTCAGGATTATCTCTCGGAAAAGTCCGCAGAGCATCTATAAAGCCAGTACGTGGGAGTGCGATTGCCTTCTCTTTCTCCAAAGACGCAAGCTTTCGATAAAATTACCGTCTTTACAGACAGACAGATACATGGCTGGTGTGAAAAAGCTCATCCAAATCGACATCGTTTCGGATACGGTATGCCCATGGTGCTTTGTGGGCAAGAGAAATCTTGAAAAAGCTTTCGAGGCATCTCGTGATCGATTCAACTTTGAGGTTAGATGGCATCCATTCCAACTTAATCCCAATGCGCCTAAAGAAGGCGTTGATAAGAGAAGTTATTACGAGGAAAAATTCGGGTCTAGTAGGTCGAAACAAATGGAGGCTCGGATGCAAGAGATTTTCAGAAGCCACGGCCTCGAATATAACCTGTCCGGACTCACGGGAAGTACTCTAGATAGCCACAGGCTTATCTATTTTGCTGGGACTCAAGGGCATGATAAGCAACACGATCTTGTGGGTGAACTGTTTCTAGGATACTTCACACAGGCAAAATACATCGGGGACAGGGAATATCTTCTGCAAAGTGCTCGAAAGGTCGGAGTAGAAGGGGCGGCAGAGTTTCTTGAAGACCCCAACAATGGGCTCAATGAGGTCAATGAAGAACTCAAGAAGTACTCAAGAAATATCAATGGAGTTCCACATTTTCTGATTAACAACGGAAAGCACGCGTTAAGTGGCGGTCAGCCACCTGAGGTGTTCTTGAGAGCTTTTGAAGCAGCTACAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DSBA

Protein Analysis

259

Amino Acids

29.6

Weight (kDa)

9.04

Isoelectric Point (pI)

48.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DSBA PF01323 54 - 253 1.1e-34 DSBA-like thioredoxin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 57
AccII CGCG 1 cut(s) 716
AccIII TCCGGA 1 cut(s) 419
AciI CCGC 3 cut(s) 32, 599, 726
AcsI RAATTY 1 cut(s) 338
AfaI GTAC 3 cut(s) 55, 436, 662
AfiI CCNNNNNNNGG 4 cut(s) 57, 305, 626, 627
AflIII ACRYGT 1 cut(s) 714
AgsI TTSAA 4 cut(s) 221, 253, 614, 761
AluBI AGCT 5 cut(s) 97, 154, 227, 755, 767
AluI AGCT 5 cut(s) 97, 154, 227, 755, 767
Alw21I GWGCWC 1 cut(s) 577
Aor13HI TCCGGA 1 cut(s) 419
AoxI GGCC 1 cut(s) 400
ApeKI GCWGC 1 cut(s) 764
ApoI RAATTY 1 cut(s) 338
Asp700I GAANNNNTTC 2 cut(s) 513, 561
AspLEI GCGC 1 cut(s) 298
AsuHPI GGTGA 1 cut(s) 518
AxyI CCTNAGG 1 cut(s) 738
BanII GRGCYC 1 cut(s) 633
BauI CACGAG 1 cut(s) 240
BbsI GAAGAC 1 cut(s) 621
Bbv12I GWGCWC 1 cut(s) 577
BccI CCATC 1 cut(s) 261
BceAI ACGGC 1 cut(s) 415
BcgI CGANNNNNNTGC 2 cut(s) 557, 591
BciVI GTATCC 2 cut(s) 172, 515
BfaI CTAG 3 cut(s) 348, 440, 518
BfuI GTATCC 2 cut(s) 172, 515
BisI GCNGC 2 cut(s) 600, 765
BlsI GCNGC 2 cut(s) 601, 766
BmcAI AGTACT 2 cut(s) 436, 662
BmsI GCATC 4 cut(s) 48, 245, 280, 366
BpiI GAAGAC 1 cut(s) 621
BpuEI CTTGAG 4 cut(s) 459, 638, 649, 769
Bsa29I ATCGAT 1 cut(s) 247
BsaAI YACGTR 1 cut(s) 57
BsaJI CCNNGG 2 cut(s) 191, 397
BsaWI WCCGGW 1 cut(s) 419
Bsc4I CCNNNNNNNGG 4 cut(s) 57, 305, 626, 627
Bse1I ACTGG 1 cut(s) 51
Bse21I CCTNAGG 1 cut(s) 738
BseAI TCCGGA 1 cut(s) 419
BseCI ATCGAT 1 cut(s) 247
BseDI CCNNGG 2 cut(s) 191, 397
BseGI GGATG 3 cut(s) 156, 271, 381
BseLI CCNNNNNNNGG 4 cut(s) 57, 305, 626, 627
BseMII CTCAG 2 cut(s) 22, 729
BseNI ACTGG 1 cut(s) 51
BseYI CCCAGC 1 cut(s) 467
Bsh1236I CGCG 1 cut(s) 716
BshFI GGCC 1 cut(s) 402
BshVI ATCGAT 1 cut(s) 247
BsiHKAI GWGCWC 1 cut(s) 577
BsiSI CCGG 1 cut(s) 420
BslFI GGGAC 2 cut(s) 484, 563
BslI CCNNNNNNNGG 4 cut(s) 57, 305, 626, 627
BsmFI GGGAC 2 cut(s) 484, 563
BsnI GGCC 1 cut(s) 402
Bsp1286I GDGCHC 2 cut(s) 577, 633
Bsp13I TCCGGA 1 cut(s) 419
Bsp143I GATC 2 cut(s) 244, 496
Bsp19I CCATGG 1 cut(s) 191
BspACI CCGC 3 cut(s) 32, 599, 726
BspANI GGCC 1 cut(s) 402
BspCNI CTCAG 2 cut(s) 21, 730
BspDI ATCGAT 1 cut(s) 247
BspEI TCCGGA 1 cut(s) 419
BspFNI CGCG 1 cut(s) 716
BsrI ACTGG 1 cut(s) 51
BssECI CCNNGG 2 cut(s) 191, 397
BssMI GATC 2 cut(s) 244, 496
BssSI CACGAG 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 191
Bst2BI CACGAG 1 cut(s) 240
Bst4CI ACNGT 3 cut(s) 114, 184, 513
BstBAI YACGTR 1 cut(s) 57
BstC8I GCNNGC 2 cut(s) 95, 714
BstDEI CTNAG 2 cut(s) 8, 738
BstDSI CCRYGG 2 cut(s) 191, 397
BstENI CCTNNNNNAGG 1 cut(s) 303
BstF5I GGATG 3 cut(s) 156, 271, 381
BstFNI CGCG 1 cut(s) 716
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 2 cut(s) 247, 499
BstMBI GATC 2 cut(s) 244, 496
BstMWI GCNNNNNNNGC 5 cut(s) 195, 204, 233, 487, 761
BstUI CGCG 1 cut(s) 716
BstV2I GAAGAC 1 cut(s) 621
Bsu15I ATCGAT 1 cut(s) 247
Bsu36I CCTNAGG 1 cut(s) 738
BsuI GTATCC 2 cut(s) 172, 515
BsuRI GGCC 1 cut(s) 402
BsuTUI ATCGAT 1 cut(s) 247
BtgI CCRYGG 2 cut(s) 191, 397
BtsCI GGATG 3 cut(s) 156, 271, 381
Cac8I GCNNGC 2 cut(s) 95, 714
CfoI GCGC 1 cut(s) 298
ClaI ATCGAT 1 cut(s) 247
CseI GACGC 1 cut(s) 99
Csp6I GTAC 3 cut(s) 54, 435, 661
CviAII CATG 3 cut(s) 136, 192, 482
CviQI GTAC 3 cut(s) 54, 435, 661
DdeI CTNAG 2 cut(s) 8, 738
DpnI GATC 2 cut(s) 246, 498
DpnII GATC 2 cut(s) 244, 496
Eco130I CCWWGG 1 cut(s) 191
Eco24I GRGCYC 1 cut(s) 633
Eco81I CCTNAGG 1 cut(s) 738
EcoNI CCTNNNNNAGG 1 cut(s) 303
EcoT14I CCWWGG 1 cut(s) 191
EcoT38I GRGCYC 1 cut(s) 633
ErhI CCWWGG 1 cut(s) 191
FaeI CATG 3 cut(s) 139, 195, 485
FaiI YATR 6 cut(s) 45, 137, 187, 193, 411, 483
FaqI GGGAC 2 cut(s) 484, 563
FatI CATG 3 cut(s) 135, 191, 481
Fnu4HI GCNGC 2 cut(s) 600, 765
FokI GGATG 3 cut(s) 143, 258, 388
FriOI GRGCYC 1 cut(s) 633
Fsp4HI GCNGC 2 cut(s) 600, 765
FspBI CTAG 3 cut(s) 348, 440, 518
GlaI GCGC 1 cut(s) 297
GluI GCNGC 2 cut(s) 600, 765
GsaI CCCAGC 1 cut(s) 471
HaeIII GGCC 1 cut(s) 402
HapII CCGG 1 cut(s) 420
HgaI GACGC 1 cut(s) 99
HhaI GCGC 1 cut(s) 298
Hin1II CATG 3 cut(s) 139, 195, 485
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HindIII AAGCTT 2 cut(s) 95, 225
HinfI GANTC 3 cut(s) 249, 423, 472
HpaII CCGG 1 cut(s) 420
HphI GGTGA 1 cut(s) 518
Hpy166II GTNNAC 1 cut(s) 509
Hpy188I TCNGA 6 cut(s) 24, 178, 375, 392, 587, 696
Hpy188III TCNNGA 9 cut(s) 10, 218, 242, 420, 440, 611, 655, 666, 748
Hpy8I GTNNAC 1 cut(s) 509
HpyAV CCTTC 3 cut(s) 83, 299, 587
HpyCH4III ACNGT 3 cut(s) 114, 184, 513
HpyCH4IV ACGT 1 cut(s) 56
HpyCH4V TGCA 2 cut(s) 379, 568
HpyF10VI GCNNNNNNNGC 5 cut(s) 195, 204, 233, 487, 761
HpyF3I CTNAG 2 cut(s) 8, 738
HpySE526I ACGT 1 cut(s) 56
Hsp92II CATG 3 cut(s) 139, 195, 485
HspAI GCGC 1 cut(s) 296
Kpn2I TCCGGA 1 cut(s) 419
Kzo9I GATC 2 cut(s) 244, 496
LpnPI CCDG 9 cut(s) 64, 126, 428, 433, 436, 453, 518, 538, 750
LweI GCATC 4 cut(s) 48, 245, 280, 366
MaeI CTAG 3 cut(s) 348, 440, 518
MaeII ACGT 1 cut(s) 56
MalI GATC 2 cut(s) 246, 498
MboI GATC 2 cut(s) 244, 496
MboII GAAGA 3 cut(s) 554, 626, 659
MhlI GDGCHC 2 cut(s) 577, 633
MluCI AATT 2 cut(s) 107, 338
MluI ACGCGT 1 cut(s) 714
MlyI GAGTC 2 cut(s) 417, 466
MmeI TCCRAC 2 cut(s) 304, 565
MnlI CCTC 7 cut(s) 226, 253, 325, 361, 413, 631, 733
MroI TCCGGA 1 cut(s) 419
MroXI GAANNNNTTC 2 cut(s) 513, 561
MseI TTAA 3 cut(s) 285, 699, 719
MspI CCGG 1 cut(s) 420
MvnI CGCG 1 cut(s) 716
MwoI GCNNNNNNNGC 5 cut(s) 195, 204, 233, 487, 761
NcoI CCATGG 1 cut(s) 191
NdeII GATC 2 cut(s) 244, 496
NlaIII CATG 3 cut(s) 139, 195, 485
PdmI GAANNNNTTC 2 cut(s) 513, 561
PfeI GAWTC 1 cut(s) 249
PflMI CCANNNNNTGG 1 cut(s) 57
PkrI GCNGC 2 cut(s) 601, 766
PleI GAGTC 2 cut(s) 417, 466
PpsI GAGTC 2 cut(s) 417, 466
Ppu21I YACGTR 1 cut(s) 57
PspFI CCCAGC 1 cut(s) 467
RsaI GTAC 3 cut(s) 55, 436, 662
RsaNI GTAC 3 cut(s) 54, 435, 661
SaqAI TTAA 3 cut(s) 285, 699, 719
SatI GCNGC 2 cut(s) 600, 765
Sau3AI GATC 2 cut(s) 244, 496
ScaI AGTACT 2 cut(s) 436, 662
SchI GAGTC 2 cut(s) 417, 466
SduI GDGCHC 2 cut(s) 577, 633
SfaNI GCATC 4 cut(s) 48, 245, 280, 366
SmlI CTYRAG 4 cut(s) 474, 653, 664, 748
SmoI CTYRAG 4 cut(s) 474, 653, 664, 748
Sse9I AATT 2 cut(s) 107, 338
SsiI CCGC 3 cut(s) 32, 599, 726
SspMI CTAG 3 cut(s) 348, 440, 518
StyI CCWWGG 1 cut(s) 191
TaaI ACNGT 3 cut(s) 114, 184, 513
TaiI ACGT 1 cut(s) 59
TaqI TCGA 7 cut(s) 101, 165, 231, 247, 356, 405, 577
TasI AATT 2 cut(s) 107, 338
TatI WGTACW 2 cut(s) 434, 660
TauI GCSGC 1 cut(s) 602
TfiI GAWTC 1 cut(s) 249
Tru1I TTAA 3 cut(s) 285, 699, 719
Tru9I TTAA 3 cut(s) 285, 699, 719
TseI GCWGC 1 cut(s) 764
TspDTI ATGAA 1 cut(s) 660
TspGWI ACGGA 1 cut(s) 720
Van91I CCANNNNNTGG 1 cut(s) 57
XagI CCTNNNNNAGG 1 cut(s) 303
XapI RAATTY 1 cut(s) 338
XbaI TCTAGA 1 cut(s) 439
XmnI GAANNNNTTC 2 cut(s) 513, 561
XspI CTAG 3 cut(s) 348, 440, 518
ZrmI AGTACT 2 cut(s) 436, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.