pycom09g02790

SPX and EXS domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0003754
Physical Location & Seq
Forward (+)
2053595 .. 2058132
4538 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1401 bp
ATGTTTGGAGGTGTTGCAACAGTGCCTTCCAATAGTCCTCACTTAAGAAAGTCGGGTACAAGTGAGCTTGAAAATGGTGCAGAGGAGGGCTTCTTGCGTCTGATAGAGGGAAACGATATGAAGGGTGGAAGCATACCAAGGAGTACTGCTGCAATAATGCCATCTCCAGCTATGTTATGGAGATTTAAGGTCCTACTGTTCTTTGCTTGGGGTTTCATTTGTTGCAAGGTTGGATGGGATTCTGTCATGAGGATGAGTGCGGATCTGATAGATTTATTTCTATATGAGGCATTTTTGTATTATAATCCTCTACTTCTTGTGGTTAACTATGATGGTTTGGTTTTGGGGAGTCAATCTTTGGGTTTTTGCTCAGGGCAATGTAAACTATGCCAAAATTATTATTTGATCGATCAAAATCATTTGACCCACAGAGAAATATGGAAGTGTGCGACATGGATGACAATTGTTGTGCCGACAAGCATGACAGCTTATCTTTATCTCTATTCGCATGGAGAAGTGTCACTTGCTGCATCACAACCAGTACTATTATATGCTGCTGTTGCAATGCTTTTGATATTCCCTTTTGACATTTTCTATTTGTCATCTCGCTTTTACTTGTTAAGGACTCTTTGGCGTATAGTTCTCCCATTACAGGCAATATCATTTTCTGACTTTTTCCTGGCTGATATTTTGACCTCCATGTCTAAGGTGCTTTCAGATTTGGAGCGATCAGTATGTCGAATGGTCCATCGACAGGTTGCCACTATTGCATGGTTTGAAGCTGACTCAGTTTGTGGAAGTCACTCTGTTGCAATCCCTGTGGTTCTTGTTTTGCCTTACCTTTTTCGTTTGTTCCAATGCCTTCGACAATATAAGGATACTGGGGAGAAAACAACCCTTCTAAATGCTTTGAAGTATTCTACTGCAGTACCTGTGATTTTTCTTTCAGCCCTCAAATATCATGTCTTCCCTGAAAAGTGGTCGACTTTTTATCGGCCCCTCTGGCTTCTCTCAGGTGTTTTGAACTCTTTGTACTCGTTCTACTGGGACGTGACTAGAGATTGGGACTTAAGTGGTTTCACTAGGATTTTCAAGTTCAGCAAGCAACAATTCTTGTCAAACCTCGTACATGGACGGAAATGGGTTTACTTCTGGGTGATAGGTAGCAACCTGATTCTGCGTTGTACCTGGACTTACAAGCTCTCTTCCCATCTCCGCCACAATTACCTTACCGTATTCGCAATCACTGCCTTGGAGATTTTCCGCCGCTTCCAATGGGTTTTCTTCCGTGTAGAAAACGAGTTGAACAAGATGAGCTCTAAATCAAACATTCAGCTTTCCATGGCCGACAATCCAAATGAGGAAGACAGATTACTCGTCTCTAATGGCCACAGCGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EXS

Protein Analysis

467

Amino Acids

53.67

Weight (kDa)

8.87

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXS PF03124 66 - 434 3.5e-83 EXS family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AasI GACNNNNNNGTC 1 cut(s) 700
AccI GTMKAC 1 cut(s) 983
AciI CCGC 5 cut(s) 260, 1216, 1264, 1267, 1395
AclWI GGATC 1 cut(s) 270
AcoI YGGCCR 2 cut(s) 1344, 1387
AfaI GTAC 7 cut(s) 58, 145, 543, 930, 1034, 1128, 1186
AfiI CCNNNNNNNGG 2 cut(s) 652, 754
AflII CTTAAG 2 cut(s) 43, 1069
AgsI TTSAA 6 cut(s) 71, 779, 913, 1024, 1093, 1306
AjiI CACGTC 1 cut(s) 1051
AjnI CCWGG 2 cut(s) 678, 1187
AluBI AGCT 7 cut(s) 67, 170, 488, 782, 1201, 1317, 1336
AluI AGCT 7 cut(s) 67, 170, 488, 782, 1201, 1317, 1336
Alw21I GWGCWC 1 cut(s) 1319
Alw26I GTCTC 1 cut(s) 1384
AlwI GGATC 1 cut(s) 270
AlwNI CAGNNNCTG 1 cut(s) 932
AoxI GGCC 3 cut(s) 995, 1344, 1387
ApeKI GCWGC 3 cut(s) 149, 527, 554
ArsI GACNNNNNNTTYG 2 cut(s) 948, 980
AspS9I GGNCC 3 cut(s) 190, 745, 996
AsuHPI GGTGA 1 cut(s) 1168
AvaII GGWCC 2 cut(s) 190, 745
BaeI ACNNNNGTAYC 2 cut(s) 912, 945
BalI TGGCCA 1 cut(s) 1389
BanII GRGCYC 1 cut(s) 1319
BarI GAAGNNNNNNTAC 2 cut(s) 1356, 1388
BbsI GAAGAC 2 cut(s) 958, 1371
Bbv12I GWGCWC 1 cut(s) 1319
BbvI GCAGC 3 cut(s) 136, 514, 541
BccI CCATC 5 cut(s) 169, 228, 326, 756, 1218
BciT130I CCWGG 2 cut(s) 680, 1189
BciVI GTATCC 1 cut(s) 871
BcoDI GTCTC 1 cut(s) 1384
BfaI CTAG 2 cut(s) 1056, 1083
BfmI CTRYAG 1 cut(s) 924
BfrI CTTAAG 2 cut(s) 43, 1069
BfuI GTATCC 1 cut(s) 871
BglI GCCNNNNNGGC 1 cut(s) 1003
BisI GCNGC 4 cut(s) 150, 528, 555, 1267
BlsI GCNGC 4 cut(s) 151, 529, 556, 1268
BmcAI AGTACT 2 cut(s) 145, 543
Bme1390I CCNGG 2 cut(s) 680, 1189
Bme18I GGWCC 2 cut(s) 190, 745
BmgBI CACGTC 1 cut(s) 1051
BmgT120I GGNCC 3 cut(s) 190, 745, 996
BmiI GGNNCC 1 cut(s) 998
BmrFI CCNGG 2 cut(s) 680, 1189
BmrI ACTGGG 2 cut(s) 891, 1054
BmsI GCATC 1 cut(s) 539
BmuI ACTGGG 2 cut(s) 891, 1054
BpiI GAAGAC 2 cut(s) 958, 1371
BpmI CTGGAG 1 cut(s) 150
Bpu10I CCTNAGC 1 cut(s) 370
Bsa29I ATCGAT 1 cut(s) 408
BsaBI GATNNNNATC 1 cut(s) 414
BsaJI CCNNGG 3 cut(s) 137, 1251, 1341
Bsc4I CCNNNNNNNGG 2 cut(s) 652, 754
Bse1I ACTGG 3 cut(s) 539, 886, 1049
Bse3DI GCAATG 2 cut(s) 383, 570
Bse8I GATNNNNATC 1 cut(s) 414
BseBI CCWGG 2 cut(s) 680, 1189
BseCI ATCGAT 1 cut(s) 408
BseDI CCNNGG 3 cut(s) 137, 1251, 1341
BseGI GGATG 3 cut(s) 239, 258, 462
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 2 cut(s) 652, 754
BseMI GCAATG 2 cut(s) 383, 570
BseMII CTCAG 3 cut(s) 384, 801, 1026
BseNI ACTGG 3 cut(s) 539, 886, 1049
BseRI GAGGAG 1 cut(s) 98
BseXI GCAGC 3 cut(s) 136, 514, 541
BsgI GTGCAG 1 cut(s) 99
BshFI GGCC 3 cut(s) 997, 1346, 1389
BshVI ATCGAT 1 cut(s) 408
BsiHKAI GWGCWC 1 cut(s) 1319
BslFI GGGAC 2 cut(s) 1061, 1079
BslI CCNNNNNNNGG 2 cut(s) 652, 754
BsmAI GTCTC 1 cut(s) 1384
BsmBI CGTCTC 1 cut(s) 1384
BsmFI GGGAC 2 cut(s) 1061, 1079
BsnI GGCC 3 cut(s) 997, 1346, 1389
Bsp1286I GDGCHC 1 cut(s) 1319
Bsp143I GATC 4 cut(s) 262, 405, 409, 728
Bsp19I CCATGG 1 cut(s) 1341
BspACI CCGC 5 cut(s) 260, 1216, 1264, 1267, 1395
BspANI GGCC 3 cut(s) 997, 1346, 1389
BspCNI CTCAG 3 cut(s) 383, 800, 1025
BspDI ATCGAT 1 cut(s) 408
BspHI TCATGA 1 cut(s) 246
BspLI GGNNCC 1 cut(s) 998
BspMAI CTGCAG 1 cut(s) 928
BspPI GGATC 1 cut(s) 270
BspTI CTTAAG 2 cut(s) 43, 1069
BsrDI GCAATG 2 cut(s) 383, 570
BsrI ACTGG 3 cut(s) 539, 886, 1049
BssECI CCNNGG 3 cut(s) 137, 1251, 1341
BssMI GATC 4 cut(s) 262, 405, 409, 728
BssT1I CCWWGG 3 cut(s) 137, 1251, 1341
Bst2UI CCWGG 2 cut(s) 680, 1189
Bst4CI ACNGT 3 cut(s) 22, 198, 1234
Bst6I CTCTTC 1 cut(s) 1210
BstAFI CTTAAG 2 cut(s) 43, 1069
BstAPI GCANNNNNTGC 1 cut(s) 1247
BstC8I GCNNGC 1 cut(s) 1103
BstDEI CTNAG 4 cut(s) 370, 705, 787, 1012
BstDSI CCRYGG 1 cut(s) 1341
BstF5I GGATG 3 cut(s) 239, 258, 462
BstKTI GATC 4 cut(s) 265, 408, 412, 731
BstMAI GTCTC 1 cut(s) 1384
BstMBI GATC 4 cut(s) 262, 405, 409, 728
BstMWI GCNNNNNNNGC 4 cut(s) 560, 767, 1003, 1247
BstNI CCWGG 2 cut(s) 680, 1189
BstSCI CCNGG 2 cut(s) 678, 1187
BstSFI CTRYAG 1 cut(s) 924
BstV1I GCAGC 3 cut(s) 136, 514, 541
BstV2I GAAGAC 2 cut(s) 958, 1371
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
Bsu15I ATCGAT 1 cut(s) 408
BsuI GTATCC 1 cut(s) 871
BsuRI GGCC 3 cut(s) 997, 1346, 1389
BsuTUI ATCGAT 1 cut(s) 408
BtgI CCRYGG 1 cut(s) 1341
BtrI CACGTC 1 cut(s) 1051
BtsCI GGATG 3 cut(s) 239, 258, 462
BtsI GCAGTG 1 cut(s) 1245
BtsIMutI CAGTG 2 cut(s) 27, 1245
Cac8I GCNNGC 1 cut(s) 1103
CaiI CAGNNNCTG 1 cut(s) 932
CciI TCATGA 1 cut(s) 246
Cfr13I GGNCC 3 cut(s) 190, 745, 996
ClaI ATCGAT 1 cut(s) 408
CseI GACGC 1 cut(s) 86
Csp6I GTAC 7 cut(s) 57, 144, 542, 929, 1033, 1127, 1185
CspCI CAANNNNNGTGG 2 cut(s) 801, 836
CviAII CATG 9 cut(s) 247, 453, 481, 509, 700, 771, 962, 1130, 1342
CviQI GTAC 7 cut(s) 57, 144, 542, 929, 1033, 1127, 1185
DdeI CTNAG 4 cut(s) 370, 705, 787, 1012
DpnI GATC 4 cut(s) 264, 407, 411, 730
DpnII GATC 4 cut(s) 262, 405, 409, 728
DrdI GACNNNNNNGTC 1 cut(s) 700
DseDI GACNNNNNNGTC 1 cut(s) 700
EaeI YGGCCR 2 cut(s) 1344, 1387
Eam1104I CTCTTC 1 cut(s) 1210
EarI CTCTTC 1 cut(s) 1210
EciI GGCGGA 2 cut(s) 1205, 1253
Ecl136II GAGCTC 1 cut(s) 1317
Eco130I CCWWGG 3 cut(s) 137, 1251, 1341
Eco24I GRGCYC 1 cut(s) 1319
Eco47I GGWCC 2 cut(s) 190, 745
Eco53kI GAGCTC 1 cut(s) 1317
EcoICRI GAGCTC 1 cut(s) 1317
EcoO109I RGGNCCY 1 cut(s) 190
EcoRII CCWGG 2 cut(s) 678, 1187
EcoT14I CCWWGG 3 cut(s) 137, 1251, 1341
EcoT38I GRGCYC 1 cut(s) 1319
ErhI CCWWGG 3 cut(s) 137, 1251, 1341
Esp3I CGTCTC 1 cut(s) 1384
FaeI CATG 9 cut(s) 250, 456, 484, 512, 703, 774, 965, 1133, 1345
FalI AAGNNNNNCTT 2 cut(s) 507, 539
FaqI GGGAC 2 cut(s) 1061, 1079
FatI CATG 9 cut(s) 246, 452, 480, 508, 699, 770, 961, 1129, 1341
FauI CCCGC 1 cut(s) 1388
FblI GTMKAC 1 cut(s) 983
Fnu4HI GCNGC 4 cut(s) 150, 528, 555, 1267
FokI GGATG 3 cut(s) 246, 265, 469
FriOI GRGCYC 1 cut(s) 1319
Fsp4HI GCNGC 4 cut(s) 150, 528, 555, 1267
FspBI CTAG 2 cut(s) 1056, 1083
GluI GCNGC 4 cut(s) 150, 528, 555, 1267
GsuI CTGGAG 1 cut(s) 150
HaeIII GGCC 3 cut(s) 997, 1346, 1389
HgaI GACGC 1 cut(s) 86
Hin1II CATG 9 cut(s) 250, 456, 484, 512, 703, 774, 965, 1133, 1345
HincII GTYRAC 2 cut(s) 325, 984
HindII GTYRAC 2 cut(s) 325, 984
HinfI GANTC 5 cut(s) 239, 349, 625, 785, 1174
HpaI GTTAAC 1 cut(s) 325
HphI GGTGA 1 cut(s) 1168
Hpy166II GTNNAC 4 cut(s) 325, 383, 984, 1147
Hpy188I TCNGA 4 cut(s) 102, 267, 670, 718
Hpy188III TCNNGA 1 cut(s) 247
Hpy8I GTNNAC 4 cut(s) 325, 383, 984, 1147
HpyAV CCTTC 4 cut(s) 36, 115, 872, 908
HpyCH4III ACNGT 3 cut(s) 22, 198, 1234
HpyCH4IV ACGT 1 cut(s) 1050
HpyCH4V TGCA 9 cut(s) 17, 80, 152, 225, 530, 563, 770, 812, 926
HpyF10VI GCNNNNNNNGC 4 cut(s) 560, 767, 1003, 1247
HpyF3I CTNAG 4 cut(s) 370, 705, 787, 1012
HpySE526I ACGT 1 cut(s) 1050
Hsp92II CATG 9 cut(s) 250, 456, 484, 512, 703, 774, 965, 1133, 1345
KspAI GTTAAC 1 cut(s) 325
Kzo9I GATC 4 cut(s) 262, 405, 409, 728
LmnI GCTCC 1 cut(s) 724
Lsp1109I GCAGC 3 cut(s) 136, 514, 541
LweI GCATC 1 cut(s) 539
MaeI CTAG 2 cut(s) 1056, 1083
MaeII ACGT 1 cut(s) 1050
MaeIII GTNAC 3 cut(s) 519, 800, 1051
MalI GATC 4 cut(s) 264, 407, 411, 730
MboI GATC 4 cut(s) 262, 405, 409, 728
MboII GAAGA 4 cut(s) 958, 1197, 1276, 1376
MfeI CAATTG 1 cut(s) 462
MflI RGATCY 1 cut(s) 262
MhlI GDGCHC 1 cut(s) 1319
MlsI TGGCCA 1 cut(s) 1389
MluCI AATT 4 cut(s) 394, 462, 1109, 1222
MluNI TGGCCA 1 cut(s) 1389
MlyI GAGTC 3 cut(s) 358, 619, 779
MmeI TCCRAC 1 cut(s) 211
Mox20I TGGCCA 1 cut(s) 1389
MscI TGGCCA 1 cut(s) 1389
MseI TTAA 5 cut(s) 44, 186, 324, 620, 1070
MslI CAYNNNNRTG 1 cut(s) 251
Msp20I TGGCCA 1 cut(s) 1389
MspA1I CMGCKG 1 cut(s) 1395
MspCI CTTAAG 2 cut(s) 43, 1069
MspR9I CCNGG 2 cut(s) 680, 1189
MunI CAATTG 1 cut(s) 462
MvaI CCWGG 2 cut(s) 680, 1189
MwoI GCNNNNNNNGC 4 cut(s) 560, 767, 1003, 1247
NcoI CCATGG 1 cut(s) 1341
NdeII GATC 4 cut(s) 262, 405, 409, 728
NlaIII CATG 9 cut(s) 250, 456, 484, 512, 703, 774, 965, 1133, 1345
NlaIV GGNNCC 1 cut(s) 998
NmuCI GTSAC 3 cut(s) 519, 800, 1051
PagI TCATGA 1 cut(s) 246
PfeI GAWTC 2 cut(s) 239, 1174
PkrI GCNGC 4 cut(s) 151, 529, 556, 1268
PleI GAGTC 3 cut(s) 357, 619, 779
PpsI GAGTC 3 cut(s) 357, 619, 779
PpuMI RGGWCCY 1 cut(s) 190
PsiI TTATAA 1 cut(s) 303
Psp124BI GAGCTC 1 cut(s) 1319
Psp5II RGGWCCY 1 cut(s) 190
Psp6I CCWGG 2 cut(s) 678, 1187
PspGI CCWGG 2 cut(s) 678, 1187
PspN4I GGNNCC 1 cut(s) 998
PspPI GGNCC 3 cut(s) 190, 745, 996
PspPPI RGGWCCY 1 cut(s) 190
PsrI GAACNNNNNNTAC 2 cut(s) 1016, 1048
PstI CTGCAG 1 cut(s) 928
PstNI CAGNNNCTG 1 cut(s) 932
PsuI RGATCY 1 cut(s) 262
RsaI GTAC 7 cut(s) 58, 145, 543, 930, 1034, 1128, 1186
RsaNI GTAC 7 cut(s) 57, 144, 542, 929, 1033, 1127, 1185
RseI CAYNNNNRTG 1 cut(s) 251
SacI GAGCTC 1 cut(s) 1319
SalI GTCGAC 1 cut(s) 982
SaqAI TTAA 5 cut(s) 44, 186, 324, 620, 1070
SatI GCNGC 4 cut(s) 150, 528, 555, 1267
Sau3AI GATC 4 cut(s) 262, 405, 409, 728
Sau96I GGNCC 3 cut(s) 190, 745, 996
ScaI AGTACT 2 cut(s) 145, 543
SchI GAGTC 3 cut(s) 358, 619, 779
ScrFI CCNGG 2 cut(s) 680, 1189
SduI GDGCHC 1 cut(s) 1319
SfaNI GCATC 1 cut(s) 539
SfcI CTRYAG 1 cut(s) 924
SinI GGWCC 2 cut(s) 190, 745
SmiMI CAYNNNNRTG 1 cut(s) 251
SmlI CTYRAG 2 cut(s) 43, 1069
SmoI CTYRAG 2 cut(s) 43, 1069
Sse9I AATT 4 cut(s) 394, 462, 1109, 1222
SsiI CCGC 5 cut(s) 260, 1216, 1264, 1267, 1395
SspMI CTAG 2 cut(s) 1056, 1083
SstI GAGCTC 1 cut(s) 1319
StyD4I CCNGG 2 cut(s) 678, 1187
StyI CCWWGG 3 cut(s) 137, 1251, 1341
TaaI ACNGT 3 cut(s) 22, 198, 1234
TaiI ACGT 1 cut(s) 1053
TaqI TCGA 5 cut(s) 408, 739, 751, 865, 983
TasI AATT 4 cut(s) 394, 462, 1109, 1222
TatI WGTACW 3 cut(s) 143, 541, 1032
TauI GCSGC 1 cut(s) 1269
TfiI GAWTC 2 cut(s) 239, 1174
Tru1I TTAA 5 cut(s) 44, 186, 324, 620, 1070
Tru9I TTAA 5 cut(s) 44, 186, 324, 620, 1070
TscAI CASTG 2 cut(s) 27, 1252
TseFI GTSAC 3 cut(s) 519, 800, 1051
TseI GCWGC 3 cut(s) 149, 527, 554
Tsp45I GTSAC 3 cut(s) 519, 800, 1051
TspDTI ATGAA 2 cut(s) 134, 205
TspGWI ACGGA 2 cut(s) 1150, 1277
TspRI CASTG 2 cut(s) 27, 1252
Vha464I CTTAAG 2 cut(s) 43, 1069
VpaK11BI GGWCC 2 cut(s) 190, 745
XcmI CCANNNNNNNNNTGG 1 cut(s) 174
XmiI GTMKAC 1 cut(s) 983
XspI CTAG 2 cut(s) 1056, 1083
ZrmI AGTACT 2 cut(s) 145, 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.