Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0011231

Physical Location & Seq

Genomic Coordinates Reverse (-)

3771074 .. 3774295

Gene Structure 3222 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom09g05060.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3222 bp

ATGTTTCGCTGCTTTCTCTCCTTCACTCCCTCTCGCTCTCACAGAAAACTTCCCCACCGTTCAAACCCTGCTCTCACAACATCAGCTGCCGCCTCCCCTGCCGTCTCAAGCCCACCGCGTGCCTCCCTATTCTTCCAATCTCAAAACCCAACTCGCCATTTGATCCCATTCAAGAGCTTGGCTGATCAGTACACATCATCCGAGCCGCAGATTAAAACACAAGATTTGGATCATGCCAGCAATGGTGCATATGGGTATCTTGCTACTCGATTCAGGGACTCGCGTACCTCAAACGACGCGCAGAACTTTCATTTGCAGATTTGCAAACTTGGGTTTGCTAATGATTTGTTTTTGTGCAATACTCTTATCAATGTTTACGTCAGAAGCGGTGCTTTGGTGGAAGCAGGCATGCTGTTCGAGGAAATGCCTGACAAGAACTCGGTTACTTGGGCTTGCTTGATTTCGGGGTATACCCAGAATGGCATGCCAAATGAGGCGTGTGCCCATTTTAAACGGATGGTTTCTGGCGGTTTTTCGCCTAGTCCTTACGCTTTTGGTAGCGTTCTTCGAGCTTGCCAGGAGTCTGGTCCTAGCAAGCTTAAGTTTGGGATGCAAACACATGGGTTGATATCTAAAACGGATCATGCTTCTGATATGGTAATGGCTAATGTGCTGATGTCAATGTATGGGAAATGTTCGGGGCCTGTTGATGATGCTTACCGGGTTTTTTGCGAGATAAAGATTAGAAATTCAATATCTTGGAACTCAATTATTTCAGTTTATTGCCAAAGAGGTGATGCAGTCTCTGCATACAAGCTTTTCTCGAGCATGCAAAAGGACTGTGCAGGTTTCAGTTTGAAACCTAATGAGTACACATTTGGTAGCTTAATAACTGCAGCTTGTTCTTTGGCTGGTTCGGGTTTGAGTTTACTTCAGCAAATGCTGACTAGCATTAAGAAATCTGGAATTTTACAGGACTTGTACGTGGGAAGTGCTTTGGTTAGTGGATTTGCAAGGTTTGGGTTAATTGATTATGCTAGGAATATTTTTGAGCAGATGAGTCAGAGGAATGCAGTGTCAATGAACGGGTTGATGGTTGCATTGGTGAGGCAGAAGCGAGGAGAAGAAGCCACTGAAGTTTTCATGGAGATGAAGGACTTGGTTGGAACAAACCTTGATTCGCTTGTGGTTCTTTTAAGTTCTTTTCCTGAATTTTTGGTTTTGGAGGAAGGGAAAAGAAGGGGAAGAGAGGTTCATGCATACATAATCGGGGCTGGTTTAATTTACAGAAAAGTTGCAATAGGTAATGGACTGGTTAATATGTATGCTAAATGTGGCGCTATTAATGATGCTTGCTCAGTTTTCAGGCACATGGTTGACAAAGACTTAATCTCATGGAATTCTCTAATCTCTGGTCTTGACCAGAATAAGCGTTTTGAAGATGCAGTCATGAACTTCCGTGACATGAGGAGATCAGAGTTGATGCCTTCAAATTTTACATTAATTAGTGCTTTGAGTTCATGTGCAAGCTTGGGGTGGATCATGTTGGGACAACAGATACATTGTGAAGCACTTAAATTGGGACTTGATTTCGATGTTTCAGTGTCAAATGCTCTTCTTGCATTATATTCCGACACTGGGTATCTTAGTGAATGCCGAAATGTTTTCTTCTTGATGCAGGAGTATGACCAAGTGTCATGGAATTCCATAATTGGGGCTTTAGCTGGTTCAGAGGCATCAGAAGCTGTTCACTATTTCTTGGACATGATGCAATCTGGGTGGGAGCTTAATAGAGTAACACTTTTGAGCATTCTTTCAGCAGTTTCATCTCTCTTACTTCCAGAGTTGGGCCAGCAGATTCATGCTGTAGTCTTAAAATACAATGCTATCGAGGACTGTGCTATTGAGAATGCACTAATTACTTTCTATGGAAAGTGTGGAGGGATAGATGACTGTGAGAGGATATTTTCTAGAATGTCTGAGAGAAGGGATGAAATAAGTTGGAATGCCATGATTTCTGGATACATACACAATGAGCTCTTGCCCAAAGCCATGGATTTGGTCTGGTTTATGATGCAAAGGGGTCAGAGATTAGACTCGTTTACCTTTGCTACCGTTCTCAGTGCTTGTGCCTCCATTGCAACATTAGAACGTGGTATGGAAGTTCATGCGTGCGGGATAAGAGCTTGTTTGGAATCTGATGTTGTAGTGGGAAGTGCACTTGTTGACATGTACTCAAAATGTGGAAGGATTGATTATGCTTCAAGGTTCCTTGAATCAATGCCAGTGAAGAATGCATATTCTTGGAATTCACTGATATCAGGTTATGCTCGTAATGGACAGGGACAAGAAGCTCTGAGACTTTTCGTGCAAATGAAGCAACAAGGTCAGATGCCAGATCATGTTACTTTTGTCGGGGTCTTATCAGCTTGTAGCCATGCAGGAATGGTGGATGAAGGGTTTCACCATTTCGAATCCATGACCAAAGTACATGGACTGGCTCCTAGGATGGAGCACTTTTCATGTATGGTTGATCTTCTTGGCAGGGCAGGAAAACTTAATATGATAGAGGACTTCATCAACAAAATGCCAATGAAGCCTAATGTTCTTATTTGGAGGACAGTTTTAGGGGCTTGTGGCCGAGCTAGTGGTTGCAACACCGAACTAGGTAGAAGGGCTGCTGAAATGCTATTAGAGTTGGAACCCCAAAACGCTACAAACTACGTGCTTCTTGCTAACATGTATGCTTCTGGAGGGAAGTGGGATGATGTGGCCAAGGCGCGGATGGCAATGAGGAAGGCATCAGCAAAGAAGGAAGCAGGTTGTAGTTGGGTCACCATGAAGGATGGTGTTCATGTGTTTGTAGCAGGAGACAAATCACACCCGGAGAAAGATTTAATATACGATAAACTGAAGGAACTCAATGGAAAAATGAGGGATGCAGGGTATGTGCCTGAAACGAAATTTGCGCTTTATGATCTTGAACAGGAGAACAAGGAAGAACTCCTGAGCTATCACAGCGAGAAGCTTGCGGTTGCATATGTTCTTACTCGCCCGTCACAGTTGCCTATAAGGATTATGAAGAACCTCCGTGTTTGTGGTGACTGCCACACCGCCTTCAAATATATATCGAAGATTGTTGGTAGGCAAATAGTATTGCGAGACTCAAACAGATTTCATCATTTTGCAGATGGCAAGTGCTCGTGCGGGGATTACTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

1074

Amino Acids

119.26

Weight (kDa)

7.82

Isoelectric Point (pI)

42.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	145 - 192	3.6e-08	PPR repeat family
PPR_2	PF13041	250 - 301	1.2e-10	PPR repeat family
PPR	PF01535	252 - 280	8.8e-06	PPR repeat
PPR_2	PF13041	462 - 509	6.3e-07	PPR repeat family
PPR_2	PF13041	664 - 711	9.1e-08	PPR repeat family
PPR_2	PF13041	765 - 812	1.5e-12	PPR repeat family
PPR	PF01535	767 - 796	1.1e-08	PPR repeat
E_motif	PF20431	886 - 945	1.3e-20	E motif
Eplus_motif	PF20430	951 - 975	7.5e-07	E+ motif
DYW_deaminase	PF14432	982 - 1073	5.1e-41	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011231)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09950 AT5G09950 AT5G09950
fragaria_vesca	FvH4_6g40670
malus_domestica	MD09G1126300.v1.1
prunus_persica	Prupe.3G198300_v2.0.a1
pyrus_communis	pycom09g05060
rosa_chinensis	RchiOBHm_Chr2g0156061 RchiOBHm_Chr2g0156111
rosa_laevigata	RLG00000020884 RLG00000020888
rosa_multiflora	Rmu_sc0000260.1_g000021 Rmu_sc0011922.1_g000009
rosa_roxburghii	Rroxscaffold_2G00093230
rosa_rugosa	Rorug02G0451500
rosa_samantha	Rh2AG518400 Rh2BG529500 Rh2CG503100 Rh2DG538700
rosa_wichuraiana	Rw2G042680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	836, 2810
AccI	GTMKAC	1 cut(s)	470
AccII	CGCG	4 cut(s)	118, 283, 299, 2782
AclWI	GGATC	4 cut(s)	157, 237, 648, 1547
AcoI	YGGCCR	2 cut(s)	2638, 2772
AcsI	RAATTY	8 cut(s)	748, 966, 1211, 1399, 1492, 1702, 2308, 2963
AcuI	CTGAAG	3 cut(s)	917, 1155, 2933
AdeI	CACNNNGTG	1 cut(s)	119
AfaI	GTAC	6 cut(s)	191, 286, 872, 983, 2234, 2490
AfiI	CCNNNNNNNGG	4 cut(s)	1638, 1713, 1847, 2781
AflII	CTTAAG	1 cut(s)	599
AflIII	ACRYGT	2 cut(s)	2229, 2739
AhdI	GACNNNNNGTC	1 cut(s)	1693
AjnI	CCWGG	1 cut(s)	576
AloI	GAACNNNNNNTCC	2 cut(s)	1236, 1268
Alw21I	GWGCWC	4 cut(s)	2040, 2221, 2517, 3203
Alw26I	GTCTC	5 cut(s)	109, 808, 2353, 2865, 3156
Alw44I	GTGCAC	1 cut(s)	2217
AlwI	GGATC	4 cut(s)	157, 237, 648, 1547
AlwNI	CAGNNNCTG	2 cut(s)	806, 1745
Ama87I	CYCGRG	1 cut(s)	823
AoxI	GGCC	4 cut(s)	701, 1849, 2638, 2772
ApaLI	GTGCAC	1 cut(s)	2217
ApeKI	GCWGC	4 cut(s)	9, 86, 896, 2678
ApoI	RAATTY	8 cut(s)	748, 966, 1211, 1399, 1492, 1702, 2308, 2963
AseI	ATTAAT	2 cut(s)	1344, 1502
Asp700I	GAANNNNTTC	2 cut(s)	1746, 2460
AspA2I	CCTAGG	1 cut(s)	2504
AspLEI	GCGC	4 cut(s)	301, 1340, 2782, 2971
AspS9I	GGNCC	3 cut(s)	587, 701, 1849
AsuC2I	CCSGG	2 cut(s)	722, 2885
AsuHPI	GGTGA	5 cut(s)	806, 1117, 2456, 2827, 3113
AsuII	TTCGAA	1 cut(s)	2472
AvaI	CYCGRG	1 cut(s)	823
AvaII	GGWCC	1 cut(s)	587
AvrII	CCTAGG	1 cut(s)	2504
BaeGI	GKGCMC	2 cut(s)	505, 2221
BaeI	ACNNNNGTAYC	2 cut(s)	268, 301
BalI	TGGCCA	1 cut(s)	2774
BanII	GRGCYC	1 cut(s)	2040
BauI	CACGAG	1 cut(s)	3202
Bbv12I	GWGCWC	4 cut(s)	2040, 2221, 2517, 3203
BbvI	GCAGC	3 cut(s)	73, 908, 2665
BccI	CCATC	6 cut(s)	511, 1087, 2503, 2779, 2840, 3185
BceAI	ACGGC	1 cut(s)	86
BcgI	CGANNNNNNTGC	6 cut(s)	397, 431, 1880, 1914, 3011, 3045
BciT130I	CCWGG	1 cut(s)	578
BciVI	GTATCC	1 cut(s)	2015
BclI	TGATCA	1 cut(s)	184
BcnI	CCSGG	2 cut(s)	722, 2885
BcoDI	GTCTC	5 cut(s)	109, 808, 2353, 2865, 3156
BfaI	CTAG	8 cut(s)	540, 591, 948, 1038, 1971, 2505, 2646, 2666
BfmI	CTRYAG	2 cut(s)	894, 1866
BfoI	RGCGCY	1 cut(s)	1341
BfrI	CTTAAG	1 cut(s)	599
BfuAI	ACCTGC	2 cut(s)	836, 2810
BfuI	GTATCC	1 cut(s)	2015
BisI	GCNGC	6 cut(s)	10, 87, 90, 206, 897, 2679
BlnI	CCTAGG	1 cut(s)	2504
BlsI	GCNGC	6 cut(s)	11, 88, 91, 207, 898, 2680
Bme1390I	CCNGG	3 cut(s)	578, 722, 2885
Bme18I	GGWCC	1 cut(s)	587
BmeRI	GACNNNNNGTC	1 cut(s)	1693
BmeT110I	CYCGRG	1 cut(s)	823
BmgT120I	GGNCC	3 cut(s)	587, 701, 1849
BmiI	GGNNCC	4 cut(s)	702, 2270, 2502, 2703
BmrFI	CCNGG	3 cut(s)	578, 722, 2885
BmrI	ACTGGG	1 cut(s)	1647
BmuI	ACTGGG	1 cut(s)	1647
BpmI	CTGGAG	1 cut(s)	2772
Bpu10I	CCTNAGC	1 cut(s)	3008
Bpu14I	TTCGAA	1 cut(s)	2472
BpuEI	CTTGAG	1 cut(s)	91
BpuMI	CCSGG	2 cut(s)	722, 2885
BsaAI	YACGTR	2 cut(s)	985, 2725
BsaBI	GATNNNNATC	1 cut(s)	228
BsaJI	CCNNGG	3 cut(s)	2052, 2504, 2775
Bsc4I	CCNNNNNNNGG	4 cut(s)	1638, 1713, 1847, 2781
Bse1I	ACTGG	5 cut(s)	1317, 1642, 2285, 2502, 3221
Bse3DI	GCAATG	3 cut(s)	247, 2136, 2796
Bse8I	GATNNNNATC	1 cut(s)	228
BseBI	CCWGG	1 cut(s)	578
BseDI	CCNNGG	3 cut(s)	2052, 2504, 2775
BseJI	GATNNNNATC	1 cut(s)	228
BseLI	CCNNNNNNNGG	4 cut(s)	1638, 1713, 1847, 2781
BseMI	GCAATG	3 cut(s)	247, 2136, 2796
BseMII	CTCAG	5 cut(s)	1371, 1971, 2134, 2348, 2999
BseNI	ACTGG	5 cut(s)	1317, 1642, 2285, 2502, 3221
BseRI	GAGGAG	2 cut(s)	1134, 1483
BseSI	GKGCMC	2 cut(s)	505, 2221
BseXI	GCAGC	3 cut(s)	73, 908, 2665
BsgI	GTGCAG	1 cut(s)	864
Bsh1236I	CGCG	4 cut(s)	118, 283, 299, 2782
BshFI	GGCC	4 cut(s)	703, 1851, 2640, 2774
BsiHKAI	GWGCWC	4 cut(s)	2040, 2221, 2517, 3203
BsiHKCI	CYCGRG	1 cut(s)	823
BsiSI	CCGG	2 cut(s)	721, 2885
BslFI	GGGAC	4 cut(s)	290, 1563, 1596, 2358
BslI	CCNNNNNNNGG	4 cut(s)	1638, 1713, 1847, 2781
BsmAI	GTCTC	5 cut(s)	109, 808, 2353, 2865, 3156
BsmBI	CGTCTC	1 cut(s)	109
BsmFI	GGGAC	4 cut(s)	290, 1563, 1596, 2358
BsmI	GAATGC	6 cut(s)	1075, 1658, 1809, 1915, 2011, 2299
BsnI	GGCC	4 cut(s)	703, 1851, 2640, 2774
BsoBI	CYCGRG	1 cut(s)	823
Bsp119I	TTCGAA	1 cut(s)	2472
Bsp1286I	GDGCHC	5 cut(s)	505, 2040, 2221, 2517, 3203
Bsp143I	GATC	9 cut(s)	162, 184, 229, 640, 1472, 1539, 2398, 2533, 2977
Bsp19I	CCATGG	1 cut(s)	2052
BspANI	GGCC	4 cut(s)	703, 1851, 2640, 2774
BspCNI	CTCAG	5 cut(s)	1370, 1972, 2133, 2349, 3000
BspFNI	CGCG	4 cut(s)	118, 283, 299, 2782
BspHI	TCATGA	1 cut(s)	1449
BspLI	GGNNCC	4 cut(s)	702, 2270, 2502, 2703
BspMAI	CTGCAG	1 cut(s)	898
BspMI	ACCTGC	2 cut(s)	836, 2810
BspPI	GGATC	4 cut(s)	157, 237, 648, 1547
BspQI	GCTCTTC	1 cut(s)	1620
BspT104I	TTCGAA	1 cut(s)	2472
BspTI	CTTAAG	1 cut(s)	599
BsrDI	GCAATG	3 cut(s)	247, 2136, 2796
BsrI	ACTGG	5 cut(s)	1317, 1642, 2285, 2502, 3221
BssECI	CCNNGG	3 cut(s)	2052, 2504, 2775
BssMI	GATC	9 cut(s)	162, 184, 229, 640, 1472, 1539, 2398, 2533, 2977
BssNAI	GTATAC	1 cut(s)	471
BssSI	CACGAG	1 cut(s)	3202
BssT1I	CCWWGG	3 cut(s)	2052, 2504, 2775
Bst1107I	GTATAC	1 cut(s)	471
Bst2BI	CACGAG	1 cut(s)	3202
Bst2UI	CCWGG	1 cut(s)	578
Bst4CI	ACNGT	7 cut(s)	59, 842, 1898, 1955, 2116, 2623, 3063
Bst6I	CTCTTC	2 cut(s)	1240, 1620
BstAFI	CTTAAG	1 cut(s)	599
BstAPI	GCANNNNNTGC	1 cut(s)	806
BstBAI	YACGTR	2 cut(s)	985, 2725
BstBI	TTCGAA	1 cut(s)	2472
BstDEI	CTNAG	6 cut(s)	1357, 1646, 1980, 2120, 2357, 3008
BstDSI	CCRYGG	1 cut(s)	2052
BstEII	GGTNACC	1 cut(s)	2833
BstFNI	CGCG	4 cut(s)	118, 283, 299, 2782
BstH2I	RGCGCY	1 cut(s)	1341
BstHHI	GCGC	4 cut(s)	301, 1340, 2782, 2971
BstKTI	GATC	9 cut(s)	165, 187, 232, 643, 1475, 1542, 2401, 2536, 2980
BstMAI	GTCTC	5 cut(s)	109, 808, 2353, 2865, 3156
BstMBI	GATC	9 cut(s)	162, 184, 229, 640, 1472, 1539, 2398, 2533, 2977
BstMWI	GCNNNNNNNGC	9 cut(s)	98, 806, 1619, 1742, 2138, 2377, 2596, 2786, 3018
BstNI	CCWGG	1 cut(s)	578
BstNSI	RCATGY	5 cut(s)	412, 487, 832, 2233, 2743
BstPI	GGTNACC	1 cut(s)	2833
BstSCI	CCNGG	3 cut(s)	576, 720, 2883
BstSFI	CTRYAG	2 cut(s)	894, 1866
BstSLI	GKGCMC	2 cut(s)	505, 2221
BstUI	CGCG	4 cut(s)	118, 283, 299, 2782
BstV1I	GCAGC	3 cut(s)	73, 908, 2665
BstXI	CCANNNNNNTGG	3 cut(s)	584, 2053, 2059
BstZ17I	GTATAC	1 cut(s)	471
BsuI	GTATCC	1 cut(s)	2015
BsuRI	GGCC	4 cut(s)	703, 1851, 2640, 2774
BtgI	CCRYGG	1 cut(s)	2052
BtsI	GCAGTG	1 cut(s)	1080
BtsIMutI	CAGTG	7 cut(s)	1080, 1131, 1608, 1635, 2128, 2292, 2312
BveI	ACCTGC	2 cut(s)	836, 2810
CaiI	CAGNNNCTG	2 cut(s)	806, 1745
CciI	TCATGA	1 cut(s)	1449
CfoI	GCGC	4 cut(s)	301, 1340, 2782, 2971
Cfr13I	GGNCC	3 cut(s)	587, 701, 1849
CseI	GACGC	1 cut(s)	305
Csp6I	GTAC	6 cut(s)	190, 285, 871, 982, 2233, 2489
CspCI	CAANNNNNGTGG	2 cut(s)	1760, 1795
CviQI	GTAC	6 cut(s)	190, 285, 871, 982, 2233, 2489
DdeI	CTNAG	6 cut(s)	1357, 1646, 1980, 2120, 2357, 3008
DpnI	GATC	9 cut(s)	164, 186, 231, 642, 1474, 1541, 2400, 2535, 2979
DpnII	GATC	9 cut(s)	162, 184, 229, 640, 1472, 1539, 2398, 2533, 2977
DraI	TTTAAA	1 cut(s)	511
DraIII	CACNNNGTG	1 cut(s)	119
DriI	GACNNNNNGTC	1 cut(s)	1693
EaeI	YGGCCR	2 cut(s)	2638, 2772
Eam1104I	CTCTTC	2 cut(s)	1240, 1620
Eam1105I	GACNNNNNGTC	1 cut(s)	1693
EarI	CTCTTC	2 cut(s)	1240, 1620
Ecl136II	GAGCTC	1 cut(s)	2038
Eco130I	CCWWGG	3 cut(s)	2052, 2504, 2775
Eco24I	GRGCYC	1 cut(s)	2040
Eco32I	GATATC	2 cut(s)	630, 2319
Eco47I	GGWCC	1 cut(s)	587
Eco53kI	GAGCTC	1 cut(s)	2038
Eco57I	CTGAAG	3 cut(s)	917, 1155, 2933
Eco88I	CYCGRG	1 cut(s)	823
Eco91I	GGTNACC	1 cut(s)	2833
EcoICRI	GAGCTC	1 cut(s)	2038
EcoO109I	RGGNCCY	1 cut(s)	701
EcoO65I	GGTNACC	1 cut(s)	2833
EcoRI	GAATTC	3 cut(s)	1399, 1702, 2308
EcoRII	CCWGG	1 cut(s)	576
EcoRV	GATATC	2 cut(s)	630, 2319
EcoT14I	CCWWGG	3 cut(s)	2052, 2504, 2775
EcoT22I	ATGCAT	2 cut(s)	1261, 2299
EcoT38I	GRGCYC	1 cut(s)	2040
ErhI	CCWWGG	3 cut(s)	2052, 2504, 2775
Esp3I	CGTCTC	1 cut(s)	109
FalI	AAGNNNNNCTT	2 cut(s)	376, 408
FaqI	GGGAC	4 cut(s)	290, 1563, 1596, 2358
FauI	CCCGC	2 cut(s)	2168, 3200
FauNDI	CATATG	2 cut(s)	250, 3040
FbaI	TGATCA	1 cut(s)	184
FblI	GTMKAC	1 cut(s)	470
Fnu4HI	GCNGC	6 cut(s)	10, 87, 90, 206, 897, 2679
FriOI	GRGCYC	1 cut(s)	2040
Fsp4HI	GCNGC	6 cut(s)	10, 87, 90, 206, 897, 2679
FspBI	CTAG	8 cut(s)	540, 591, 948, 1038, 1971, 2505, 2646, 2666
GlaI	GCGC	4 cut(s)	300, 1339, 2781, 2970
GluI	GCNGC	6 cut(s)	10, 87, 90, 206, 897, 2679
GsuI	CTGGAG	1 cut(s)	2772
HaeII	RGCGCY	1 cut(s)	1341
HaeIII	GGCC	4 cut(s)	703, 1851, 2640, 2774
HapII	CCGG	2 cut(s)	721, 2885
HgaI	GACGC	1 cut(s)	305
HhaI	GCGC	4 cut(s)	301, 1340, 2782, 2971
Hin6I	GCGC	4 cut(s)	299, 1338, 2780, 2969
HinP1I	GCGC	4 cut(s)	299, 1338, 2780, 2969
HincII	GTYRAC	2 cut(s)	1378, 2227
HindII	GTYRAC	2 cut(s)	1378, 2227
HindIII	AAGCTT	4 cut(s)	596, 815, 1528, 3026
HpaII	CCGG	2 cut(s)	721, 2885
HphI	GGTGA	5 cut(s)	806, 1117, 2456, 2827, 3113
Hpy99I	CGWCG	1 cut(s)	299
HpyCH4III	ACNGT	7 cut(s)	59, 842, 1898, 1955, 2116, 2623, 3063
HpyCH4IV	ACGT	4 cut(s)	378, 984, 2152, 2724
HpyF10VI	GCNNNNNNNGC	9 cut(s)	98, 806, 1619, 1742, 2138, 2377, 2596, 2786, 3018
HpyF3I	CTNAG	6 cut(s)	1357, 1646, 1980, 2120, 2357, 3008
HpySE526I	ACGT	4 cut(s)	378, 984, 2152, 2724
HspAI	GCGC	4 cut(s)	299, 1338, 2780, 2969
Ksp22I	TGATCA	1 cut(s)	184
Kzo9I	GATC	9 cut(s)	162, 184, 229, 640, 1472, 1539, 2398, 2533, 2977
LguI	GCTCTTC	1 cut(s)	1620
LmnI	GCTCC	3 cut(s)	1783, 2506, 2512
Lsp1109I	GCAGC	3 cut(s)	73, 908, 2665
MaeI	CTAG	8 cut(s)	540, 591, 948, 1038, 1971, 2505, 2646, 2666
MaeII	ACGT	4 cut(s)	378, 984, 2152, 2724
MaeIII	GTNAC	7 cut(s)	442, 1460, 1795, 2404, 2833, 3057, 3101
MalI	GATC	9 cut(s)	164, 186, 231, 642, 1474, 1541, 2400, 2535, 2979
MboI	GATC	9 cut(s)	162, 184, 229, 640, 1472, 1539, 2398, 2533, 2977
MhlI	GDGCHC	5 cut(s)	505, 2040, 2221, 2517, 3203
MlsI	TGGCCA	1 cut(s)	2774
MluNI	TGGCCA	1 cut(s)	2774
MlyI	GAGTC	5 cut(s)	272, 590, 1069, 2090, 3158
MmeI	TCCRAC	4 cut(s)	1144, 1656, 1982, 2679
Mox20I	TGGCCA	1 cut(s)	2774
Mph1103I	ATGCAT	2 cut(s)	1261, 2299
MroXI	GAANNNNTTC	2 cut(s)	1746, 2460
MscI	TGGCCA	1 cut(s)	2774
MslI	CAYNNNNRTG	1 cut(s)	1148
Msp20I	TGGCCA	1 cut(s)	2774
MspA1I	CMGCKG	1 cut(s)	86
MspCI	CTTAAG	1 cut(s)	599
MspI	CCGG	2 cut(s)	721, 2885
MspR9I	CCNGG	3 cut(s)	578, 722, 2885
Mva1269I	GAATGC	6 cut(s)	1075, 1658, 1809, 1915, 2011, 2299
MvaI	CCWGG	1 cut(s)	578
MvnI	CGCG	4 cut(s)	118, 283, 299, 2782
MwoI	GCNNNNNNNGC	9 cut(s)	98, 806, 1619, 1742, 2138, 2377, 2596, 2786, 3018
NciI	CCSGG	2 cut(s)	722, 2885
NcoI	CCATGG	1 cut(s)	2052
NdeI	CATATG	2 cut(s)	250, 3040
NdeII	GATC	9 cut(s)	162, 184, 229, 640, 1472, 1539, 2398, 2533, 2977
NlaIV	GGNNCC	4 cut(s)	702, 2270, 2502, 2703
NmeAIII	GCCGAG	1 cut(s)	2666
NmuCI	GTSAC	4 cut(s)	1460, 2833, 3057, 3101
NsiI	ATGCAT	2 cut(s)	1261, 2299
NspI	RCATGY	5 cut(s)	412, 487, 832, 2233, 2743
NspV	TTCGAA	1 cut(s)	2472
PaeI	GCATGC	3 cut(s)	412, 487, 832
PaeR7I	CTCGAG	1 cut(s)	823
PagI	TCATGA	1 cut(s)	1449
PciI	ACATGT	2 cut(s)	2229, 2739
PciSI	GCTCTTC	1 cut(s)	1620
PctI	GAATGC	6 cut(s)	1075, 1658, 1809, 1915, 2011, 2299
PdmI	GAANNNNTTC	2 cut(s)	1746, 2460
PfeI	GAWTC	6 cut(s)	270, 1178, 1858, 2195, 2276, 2474
PkrI	GCNGC	6 cut(s)	11, 88, 91, 207, 898, 2680
PleI	GAGTC	5 cut(s)	272, 589, 1068, 2090, 3158
PpsI	GAGTC	5 cut(s)	272, 589, 1068, 2090, 3158
Ppu21I	YACGTR	2 cut(s)	985, 2725
PscI	ACATGT	2 cut(s)	2229, 2739
PshBI	ATTAAT	2 cut(s)	1344, 1502
Psp124BI	GAGCTC	1 cut(s)	2040
Psp6I	CCWGG	1 cut(s)	576
PspEI	GGTNACC	1 cut(s)	2833
PspGI	CCWGG	1 cut(s)	576
PspN4I	GGNNCC	4 cut(s)	702, 2270, 2502, 2703
PspPI	GGNCC	3 cut(s)	587, 701, 1849
PstI	CTGCAG	1 cut(s)	898
PstNI	CAGNNNCTG	2 cut(s)	806, 1745
PvuII	CAGCTG	1 cut(s)	86
RsaI	GTAC	6 cut(s)	191, 286, 872, 983, 2234, 2490
RsaNI	GTAC	6 cut(s)	190, 285, 871, 982, 2233, 2489
RseI	CAYNNNNRTG	1 cut(s)	1148
SacI	GAGCTC	1 cut(s)	2040
SapI	GCTCTTC	1 cut(s)	1620
SatI	GCNGC	6 cut(s)	10, 87, 90, 206, 897, 2679
Sau3AI	GATC	9 cut(s)	162, 184, 229, 640, 1472, 1539, 2398, 2533, 2977
Sau96I	GGNCC	3 cut(s)	587, 701, 1849
SchI	GAGTC	5 cut(s)	272, 590, 1069, 2090, 3158
ScrFI	CCNGG	3 cut(s)	578, 722, 2885
SduI	GDGCHC	5 cut(s)	505, 2040, 2221, 2517, 3203
SfcI	CTRYAG	2 cut(s)	894, 1866
Sfr274I	CTCGAG	1 cut(s)	823
SfuI	TTCGAA	1 cut(s)	2472
SinI	GGWCC	1 cut(s)	587
SlaI	CTCGAG	1 cut(s)	823
SmiMI	CAYNNNNRTG	1 cut(s)	1148
SmlI	CTYRAG	3 cut(s)	106, 599, 823
SmoI	CTYRAG	3 cut(s)	106, 599, 823
SphI	GCATGC	3 cut(s)	412, 487, 832
SspI	AATATT	1 cut(s)	1045
SspMI	CTAG	8 cut(s)	540, 591, 948, 1038, 1971, 2505, 2646, 2666
SstI	GAGCTC	1 cut(s)	2040
StyD4I	CCNGG	3 cut(s)	576, 720, 2883
StyI	CCWWGG	3 cut(s)	2052, 2504, 2775
TaaI	ACNGT	7 cut(s)	59, 842, 1898, 1955, 2116, 2623, 3063
TaiI	ACGT	4 cut(s)	381, 987, 2155, 2727
TaqI	TCGA	8 cut(s)	268, 417, 568, 824, 1593, 1890, 2472, 3131
TatI	WGTACW	4 cut(s)	189, 870, 2232, 2488
TauI	GCSGC	2 cut(s)	92, 208
TfiI	GAWTC	6 cut(s)	270, 1178, 1858, 2195, 2276, 2474
TscAI	CASTG	7 cut(s)	1080, 1138, 1608, 1642, 2128, 2292, 2319
TseFI	GTSAC	4 cut(s)	1460, 2833, 3057, 3101
TseI	GCWGC	4 cut(s)	9, 86, 896, 2678
Tsp45I	GTSAC	4 cut(s)	1460, 2833, 3057, 3101
TspGWI	ACGGA	4 cut(s)	529, 653, 1448, 3080
TspRI	CASTG	7 cut(s)	1080, 1138, 1608, 1642, 2128, 2292, 2319
Vha464I	CTTAAG	1 cut(s)	599
VneI	GTGCAC	1 cut(s)	2217
VpaK11BI	GGWCC	1 cut(s)	587
VspI	ATTAAT	2 cut(s)	1344, 1502
XapI	RAATTY	8 cut(s)	748, 966, 1211, 1399, 1492, 1702, 2308, 2963
XbaI	TCTAGA	1 cut(s)	1970
XceI	RCATGY	5 cut(s)	412, 487, 832, 2233, 2743
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2782
XhoI	CTCGAG	1 cut(s)	823
XmaJI	CCTAGG	1 cut(s)	2504
XmiI	GTMKAC	1 cut(s)	470
XmnI	GAANNNNTTC	2 cut(s)	1746, 2460
XspI	CTAG	8 cut(s)	540, 591, 948, 1038, 1971, 2505, 2646, 2666
Zsp2I	ATGCAT	2 cut(s)	1261, 2299

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.