Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0011231

Physical Location & Seq

Genomic Coordinates Reverse (-)

14649511 .. 14653327

Gene Structure 3817 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00093230.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3132 bp

ATGTTTCGCTCTTTCCTCACTCACAAAGGAAAACTCCGCTGTTCACACCCTGCTTTCACTTCTCTCGCCGCTTCACCTCTCGTCTCAAACTCAGTTCACCACAGCCAATCATCTCCGCCCCACATCGAAACCCCACTCCCAAATTCCAATGCAACTTACGGCTTTCTTCTCAATCGGTTCAAGGACTCCCGCACCTCAAATGAAGCCGAAAGCTTTCATTTGCGGATTTTCAAACATGGCTTCACTCAAGATTTGTTCTTGTGCAATACCCTTATCAACGTTTATGTCAAAATTGGTGCTTTCGCTAATGCACGTAAGCTGTTCGACGAAATGCCGGAGAAGAACTCGGTTTCTTGGGCTTGCTTGATTTCCGGGTACGCCCGGAGCGGTGTGCCAGAGGAGGCTTGTGTGCATTTTAAAAGGATGGTTTGTGATGGTTTGGTGCCGAGTGTTTATGCTTTTGGGAGTGTTCTAAAGGCTTGCCAGGAGTTGGGCGCTTGCAAGCTTAAGTTTGGGATACAAATTCATGGGTTGTTATCGAAAATGCATGATTCGGATGTGTTGTTAGCTAATGTGGTGATGTCAATGTATGGGAGATGTTTGGGTTCTGCGGATGATGCTTACCGGGCGTTTTGTGAGATCAAGTTTAGAAATTTAGTGTCCTGGAATTCTATTATTTCGGTCTACTGCCAAAGAGGGGATGCAGTCTCTGCTTATAAGCTTTTCTCAAACATGCAAAAGGATGTTGTGGATTTTGATTTGCAACCGAATGAGTATACTTTCGGTAGCTTGGTACCTGCCTGTTGTGGTTTGGCTGATTCTGGTTTGGTTTTGCTTCAGCAAATGCTGAGCAGGGTCAGCAAAGCTGGATTTTTACAGGACTTGTACGTAGGAAGTGCTTTGGTTAGTGGATTTGCGAGGGTTGGATTAGTCGATTATGCCAGGAAGATTTTTGAGCAGATGGGTGAGAGGAATGTAGTTTCGATGAATGGGTTGATGGTTGGACTGGTGAGGCAGAAGCGAGGAGAAGAAGCCACTCAAGTTTTCATGGAGATGAAGGACTTGGTTGGAATAAATATTGATTCACTTGTGGTTCTTTTAAGTTCATTCACTGAATTTTCTGCTTTAGAGGAAGGGAAAAGAAAGGGAAGAGAAGTTCATGCATATGTAATTGCAACTGGCTTGATTAACAGCAAGGTTGCAATAGAGAATGGGCTTGTAAATATGTATGCTAAATGTGGTGCTATTGATGATGCTTGTTCAGTTTTCAGGCTCATGGTAGACAGAGACTTAATCTCATGGAACTCTTTGATCTCTGGTCTTGACCAGAATGAGCGGTTTGAAGATGCAGTAATGAAGTTCTGTGAAATGAGAAAATCTGAATTGAAGCCTTCAATTTTCACATTAATTAGTGCTTTGAGTTCATGTGCAAGCTTGGAGTGGATCATGATGGGACAGCAAATACATTGTGAAGCACTTAAATTGGGACTTGATTTGGATGTTTCGGTTTCAAATGCTCTTCTTGCATTGTATTCTGACACAGGGTGTCTTAATGAATCCCAGAATGTTTTCTTCTTGATGCAGGAGTATGACCAAGTGTCGTGGAATTCCATAGTTGGGGCTTTAGCTAGTTCAGAGGCTTCAGTTTTTGAAGCTGTAGAATATTTATTGGAAATGATGCGATCTGGATGGGAACTTAATAGAGTAACATTTATTAGCATTCTTTCTGCAGTTTCATCCCTCTCACTTCACGAGCTAGGCCAGCAGATTCATGCTGTAGTTTTAAAATACAATGCTGCAAAGGACTGTGCTATTGAGAATGCACTTATTGCTTGCTATGGAAAGTGTGGACAGATAGATGACTGTGAGAAGATATTTTCTAGAATGCCTGAAAGAAGGGATGAAGTAAGTTGGAATTCCATGATTTCTGGATACATACATAATGAGCTCTTGCCCAAGGCCATGGATTTGGTCTGGTTTATGATTGAGAGGGGTCAGAAATTAGACTCTTTTACTTTTGCTACTGTTCTGAGTGCTTGTGCTTCAGTTGCAACATTAGAGCGTGGGATGGAAGTTCATGCTTGTGCGGTAAGAGCTTATTTGGAATCTGATGTTGTAGTTGGGAGTGCACTCGTCGACATGTATTCAAAGTGTGGAAGGATTGATTATGCTTCAAGATTCTTTGAGTTGATGCCAGTAAGGAATGTGTATTCCTGGAATTCATTAATATCAGGATATGCTCGCAATGGAGATGGACAGAAAGCTCTGAGGCTTTTCACACAAATGAAGCAACAAGATCAGCCACCAGATCACGTTACCTTTGTTGGGGTCCTATCAGCTTGTAGCCATGCAGGGCTCGTGGATGAAGGGTTTCAGCATTTCAATTCCATGAGTAAAGTACATGGACTGGCTCCTAGGATGGAGCACTTTTCATGTATGGTTGATCTTCTCGGAAGGGCAGGAAAAATTAATATGATAGAGGACTTCATCAAAAAAATGCCGATGAAGCCTAATGTGCTTATTTGGAGGACAGTTTTAGGGGCTTGTTGCCGAGGCAATGGTCGTAACACAGAGCTAGGCAGGATGGCTGGTGAAATGCTATTGGAGTTGGAACCCCAAAATGCTGTGAACTATGTGCTTCTTGCTAACATGTATGCTTCTGGTGGGAAGTGGGATGACGTGGCAAAGGCTAGGATGACAATGAGAAAGGCAGCAGTGAAGAAGGAAGCTGGGTGCAGTTGGGTCACCATGAAAGATGGAGTTCATGTTTTTGTAGCTGGGGACAAATTACACCCAGAGAAAGATTTGATATATGAAAAACTGAAGGAACTTAACAAGAAAATGAGGGATGCTGGATATGTGCCTGAAACAAAATTTGCACTATATGATCTCGAACTGGAGAACAAGGAAGAACTCCTGAGTTATCACAGTGAAAAGCTTGCTGTTGCATATGTTCTTACTCGCCAGTCACAGTTGCCTATAAGGATTATGAAAAACCTTCGGGTTTGTGGTGACTGCCACACTGCCTTCAAATACATATCAAATATTGTTGGCAGGCAAATAGTATTACGCGATTCAAACAGATTTCATCACTTTCAAGATGGCAAGTGTTCTTGTGGGGATTTTTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

1043

Amino Acids

116.51

Weight (kDa)

6.83

Isoelectric Point (pI)

39.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	114 - 161	3e-07	PPR repeat family
PPR_2	PF13041	218 - 269	1.1e-10	PPR repeat family
PPR	PF01535	220 - 247	9.5e-06	PPR repeat
PPR_2	PF13041	430 - 477	5.7e-07	PPR repeat family
PPR_2	PF13041	634 - 681	1.3e-08	PPR repeat family
PPR_2	PF13041	734 - 782	1.2e-12	PPR repeat family
PPR_1	PF12854	734 - 763	8.1e-06	PPR repeat
PPR	PF01535	737 - 764	5.5e-08	PPR repeat
E_motif	PF20431	856 - 915	2.1e-19	E motif
Eplus_motif	PF20430	921 - 948	6.4e-06	E+ motif
DYW_deaminase	PF14432	952 - 1043	6.4e-40	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011231)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09950 AT5G09950 AT5G09950
fragaria_vesca	FvH4_6g40670
malus_domestica	MD09G1126300.v1.1
prunus_persica	Prupe.3G198300_v2.0.a1
pyrus_communis	pycom09g05060
rosa_chinensis	RchiOBHm_Chr2g0156061 RchiOBHm_Chr2g0156111
rosa_laevigata	RLG00000020884 RLG00000020888
rosa_multiflora	Rmu_sc0000260.1_g000021 Rmu_sc0011922.1_g000009
rosa_roxburghii	Rroxscaffold_2G00093230
rosa_rugosa	Rorug02G0451500
rosa_samantha	Rh2AG518400 Rh2BG529500 Rh2CG503100 Rh2DG538700
rosa_wichuraiana	Rw2G042680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	717
Acc36I	ACCTGC	1 cut(s)	805
Acc65I	GGTACC	1 cut(s)	793
AccB1I	GGYRCC	2 cut(s)	442, 793
AccB7I	CCANNNNNTGG	1 cut(s)	490
AccBSI	CCGCTC	2 cut(s)	387, 1336
AccI	GTMKAC	4 cut(s)	684, 776, 1281, 2136
AccII	CGCG	1 cut(s)	3072
AciI	CCGC	9 cut(s)	37, 69, 116, 190, 223, 387, 611, 1336, 2087
AclI	AACGTT	1 cut(s)	279
AclWI	GGATC	1 cut(s)	1451
AcsI	RAATTY	9 cut(s)	142, 522, 652, 667, 1115, 1606, 1915, 2218, 2873
AcuI	CTGAAG	4 cut(s)	821, 1626, 2028, 2843
AdeI	CACNNNGTG	1 cut(s)	1545
AfaI	GTAC	4 cut(s)	377, 795, 887, 2400
AfiI	CCNNNNNNNGG	4 cut(s)	490, 697, 1617, 2325
AflII	CTTAAG	1 cut(s)	506
AflIII	ACRYGT	2 cut(s)	2139, 2649
AhdI	GACNNNNNGTC	1 cut(s)	1597
AjiI	CACGTC	1 cut(s)	2680
AjnI	CCWGG	4 cut(s)	483, 662, 941, 2213
AloI	GAACNNNNNNTCC	2 cut(s)	1140, 1172
Alw21I	GWGCWC	3 cut(s)	1950, 2131, 2427
Alw26I	GTCTC	3 cut(s)	88, 712, 1281
Alw44I	GTGCAC	1 cut(s)	2127
AlwI	GGATC	1 cut(s)	1451
AlwNI	CAGNNNCTG	1 cut(s)	710
AoxI	GGCC	2 cut(s)	1759, 1959
ApaLI	GTGCAC	1 cut(s)	2127
ApeKI	GCWGC	2 cut(s)	1796, 2711
ApoI	RAATTY	9 cut(s)	142, 522, 652, 667, 1115, 1606, 1915, 2218, 2873
AseI	ATTAAT	3 cut(s)	1406, 2225, 2469
Asp700I	GAANNNNTTC	3 cut(s)	213, 1568, 2370
Asp718I	GGTACC	1 cut(s)	793
AspA2I	CCTAGG	1 cut(s)	2414
AspLEI	GCGC	1 cut(s)	497
AspS9I	GGNCC	1 cut(s)	2329
AsuC2I	CCSGG	3 cut(s)	373, 382, 626
AsuHPI	GGTGA	8 cut(s)	66, 89, 589, 977, 1021, 2603, 2737, 3023
AvaII	GGWCC	1 cut(s)	2329
AvrII	CCTAGG	1 cut(s)	2414
BaeGI	GKGCMC	1 cut(s)	2131
BanI	GGYRCC	2 cut(s)	442, 793
BanII	GRGCYC	2 cut(s)	1950, 2358
BauI	CACGAG	2 cut(s)	1751, 2357
Bbv12I	GWGCWC	3 cut(s)	1950, 2131, 2427
BbvI	GCAGC	2 cut(s)	1783, 2723
BceAI	ACGGC	1 cut(s)	175
BciT130I	CCWGG	4 cut(s)	485, 664, 943, 2215
BciVI	GTATCC	2 cut(s)	510, 1925
BcnI	CCSGG	3 cut(s)	373, 382, 626
BcoDI	GTCTC	3 cut(s)	88, 712, 1281
BfaI	CTAG	6 cut(s)	1629, 1757, 1881, 2415, 2576, 2691
BfmI	CTRYAG	3 cut(s)	1656, 1728, 1776
BfoI	RGCGCY	1 cut(s)	498
BfrI	CTTAAG	1 cut(s)	506
BfuAI	ACCTGC	1 cut(s)	805
BfuI	GTATCC	2 cut(s)	510, 1925
BisI	GCNGC	3 cut(s)	69, 1797, 2712
BlnI	CCTAGG	1 cut(s)	2414
BlpI	GCTNAGC	1 cut(s)	848
BlsI	GCNGC	3 cut(s)	70, 1798, 2713
Bme1390I	CCNGG	7 cut(s)	373, 382, 485, 626, 664, 943, 2215
Bme18I	GGWCC	1 cut(s)	2329
BmeRI	GACNNNNNGTC	1 cut(s)	1597
BmgBI	CACGTC	1 cut(s)	2680
BmgT120I	GGNCC	1 cut(s)	2329
BmiI	GGNNCC	5 cut(s)	444, 795, 2330, 2412, 2613
BmrFI	CCNGG	7 cut(s)	373, 382, 485, 626, 664, 943, 2215
BmsI	GCATC	8 cut(s)	607, 691, 1243, 1336, 1569, 1668, 2181, 2839
BplI	GAGNNNNNCTC	2 cut(s)	329, 361
BpmI	CTGGAG	1 cut(s)	2918
Bpu1102I	GCTNAGC	1 cut(s)	848
BpuEI	CTTGAG	2 cut(s)	231, 1023
BpuMI	CCSGG	3 cut(s)	373, 382, 626
BsaAI	YACGTR	2 cut(s)	314, 889
BsaJI	CCNNGG	4 cut(s)	1956, 1962, 2414, 2551
BsaXI	ACNNNNNCTCC	2 cut(s)	2751, 2781
Bsc4I	CCNNNNNNNGG	4 cut(s)	490, 697, 1617, 2325
Bse1I	ACTGG	6 cut(s)	1011, 1183, 2195, 2412, 2901, 2965
Bse3DI	GCAATG	2 cut(s)	2251, 2563
BseBI	CCWGG	4 cut(s)	485, 664, 943, 2215
BseDI	CCNNGG	4 cut(s)	1956, 1962, 2414, 2551
BseLI	CCNNNNNNNGG	4 cut(s)	490, 697, 1617, 2325
BseMI	GCAATG	2 cut(s)	2251, 2563
BseMII	CTCAG	5 cut(s)	105, 839, 2021, 2258, 2909
BseNI	ACTGG	6 cut(s)	1011, 1183, 2195, 2412, 2901, 2965
BseRI	GAGGAG	2 cut(s)	413, 1038
BseSI	GKGCMC	1 cut(s)	2131
BseXI	GCAGC	2 cut(s)	1783, 2723
BseYI	CCCAGC	2 cut(s)	2729, 2777
BsgI	GTGCAG	1 cut(s)	2755
Bsh1236I	CGCG	1 cut(s)	3072
BshFI	GGCC	2 cut(s)	1761, 1961
BshNI	GGYRCC	2 cut(s)	442, 793
BsiHKAI	GWGCWC	3 cut(s)	1950, 2131, 2427
BsiSI	CCGG	4 cut(s)	335, 372, 382, 625
BslFI	GGGAC	3 cut(s)	1467, 1500, 2795
BslI	CCNNNNNNNGG	4 cut(s)	490, 697, 1617, 2325
BsmAI	GTCTC	3 cut(s)	88, 712, 1281
BsmBI	CGTCTC	1 cut(s)	88
BsmFI	GGGAC	3 cut(s)	1467, 1500, 2795
BsmI	GAATGC	3 cut(s)	1719, 1825, 1890
BsnI	GGCC	2 cut(s)	1761, 1961
Bsp1286I	GDGCHC	4 cut(s)	1950, 2131, 2358, 2427
Bsp143I	GATC	8 cut(s)	639, 1311, 1443, 1682, 2296, 2308, 2443, 2887
Bsp1720I	GCTNAGC	1 cut(s)	848
Bsp19I	CCATGG	1 cut(s)	1962
BspACI	CCGC	9 cut(s)	37, 69, 116, 190, 223, 387, 611, 1336, 2087
BspANI	GGCC	2 cut(s)	1761, 1961
BspCNI	CTCAG	5 cut(s)	104, 840, 2022, 2259, 2910
BspFNI	CGCG	1 cut(s)	3072
BspHI	TCATGA	1 cut(s)	1446
BspLI	GGNNCC	5 cut(s)	444, 795, 2330, 2412, 2613
BspMAI	CTGCAG	1 cut(s)	1732
BspMI	ACCTGC	1 cut(s)	805
BspPI	GGATC	1 cut(s)	1451
BspQI	GCTCTTC	1 cut(s)	1524
BspT107I	GGYRCC	2 cut(s)	442, 793
BspTI	CTTAAG	1 cut(s)	506
BsrBI	CCGCTC	2 cut(s)	387, 1336
BsrDI	GCAATG	2 cut(s)	2251, 2563
BsrI	ACTGG	6 cut(s)	1011, 1183, 2195, 2412, 2901, 2965
BssECI	CCNNGG	4 cut(s)	1956, 1962, 2414, 2551
BssMI	GATC	8 cut(s)	639, 1311, 1443, 1682, 2296, 2308, 2443, 2887
BssNAI	GTATAC	1 cut(s)	777
BssSI	CACGAG	2 cut(s)	1751, 2357
BssT1I	CCWWGG	3 cut(s)	1956, 1962, 2414
Bst1107I	GTATAC	1 cut(s)	777
Bst2BI	CACGAG	2 cut(s)	1751, 2357
Bst2UI	CCWGG	4 cut(s)	485, 664, 943, 2215
Bst4CI	ACNGT	6 cut(s)	1808, 1865, 2026, 2533, 2930, 2973
Bst6I	CTCTTC	2 cut(s)	1144, 1524
BstAFI	CTTAAG	1 cut(s)	506
BstAPI	GCANNNNNTGC	2 cut(s)	710, 1829
BstBAI	YACGTR	2 cut(s)	314, 889
BstDEI	CTNAG	5 cut(s)	91, 848, 2030, 2267, 2918
BstDSI	CCRYGG	1 cut(s)	1962
BstEII	GGTNACC	1 cut(s)	2743
BstFNI	CGCG	1 cut(s)	3072
BstH2I	RGCGCY	1 cut(s)	498
BstHHI	GCGC	1 cut(s)	497
BstKTI	GATC	8 cut(s)	642, 1314, 1446, 1685, 2299, 2311, 2446, 2890
BstMAI	GTCTC	3 cut(s)	88, 712, 1281
BstMBI	GATC	8 cut(s)	639, 1311, 1443, 1682, 2296, 2308, 2443, 2887
BstNI	CCWGG	4 cut(s)	485, 664, 943, 2215
BstNSI	RCATGY	3 cut(s)	736, 2143, 2653
BstPI	GGTNACC	1 cut(s)	2743
BstSCI	CCNGG	7 cut(s)	371, 380, 483, 624, 662, 941, 2213
BstSFI	CTRYAG	3 cut(s)	1656, 1728, 1776
BstSLI	GKGCMC	1 cut(s)	2131
BstSNI	TACGTA	1 cut(s)	889
BstUI	CGCG	1 cut(s)	3072
BstV1I	GCAGC	2 cut(s)	1783, 2723
BstXI	CCANNNNNNTGG	2 cut(s)	1963, 1969
BstZ17I	GTATAC	1 cut(s)	777
BsuI	GTATCC	2 cut(s)	510, 1925
BsuRI	GGCC	2 cut(s)	1761, 1961
BtgI	CCRYGG	1 cut(s)	1962
BtrI	CACGTC	1 cut(s)	2680
BtsI	GCAGTG	2 cut(s)	2721, 3021
BtsIMutI	CAGTG	4 cut(s)	1110, 2721, 2935, 3021
BveI	ACCTGC	1 cut(s)	805
CaiI	CAGNNNCTG	1 cut(s)	710
CciI	TCATGA	1 cut(s)	1446
CfoI	GCGC	1 cut(s)	497
Cfr13I	GGNCC	1 cut(s)	2329
Csp6I	GTAC	4 cut(s)	376, 794, 886, 2399
CspCI	CAANNNNNGTGG	2 cut(s)	1583, 1618
CviQI	GTAC	4 cut(s)	376, 794, 886, 2399
DdeI	CTNAG	5 cut(s)	91, 848, 2030, 2267, 2918
DpnI	GATC	8 cut(s)	641, 1313, 1445, 1684, 2298, 2310, 2445, 2889
DpnII	GATC	8 cut(s)	639, 1311, 1443, 1682, 2296, 2308, 2443, 2887
DraI	TTTAAA	2 cut(s)	418, 1785
DraIII	CACNNNGTG	1 cut(s)	1545
DriI	GACNNNNNGTC	1 cut(s)	1597
Eam1104I	CTCTTC	2 cut(s)	1144, 1524
Eam1105I	GACNNNNNGTC	1 cut(s)	1597
EarI	CTCTTC	2 cut(s)	1144, 1524
EciI	GGCGGA	1 cut(s)	105
Ecl136II	GAGCTC	1 cut(s)	1948
Eco105I	TACGTA	1 cut(s)	889
Eco130I	CCWWGG	3 cut(s)	1956, 1962, 2414
Eco24I	GRGCYC	2 cut(s)	1950, 2358
Eco47I	GGWCC	1 cut(s)	2329
Eco53kI	GAGCTC	1 cut(s)	1948
Eco57I	CTGAAG	4 cut(s)	821, 1626, 2028, 2843
Eco91I	GGTNACC	1 cut(s)	2743
EcoICRI	GAGCTC	1 cut(s)	1948
EcoO109I	RGGNCCY	1 cut(s)	2329
EcoO65I	GGTNACC	1 cut(s)	2743
EcoRI	GAATTC	4 cut(s)	667, 1606, 1915, 2218
EcoRII	CCWGG	4 cut(s)	483, 662, 941, 2213
EcoT14I	CCWWGG	3 cut(s)	1956, 1962, 2414
EcoT22I	ATGCAT	2 cut(s)	549, 1165
EcoT38I	GRGCYC	2 cut(s)	1950, 2358
ErhI	CCWWGG	3 cut(s)	1956, 1962, 2414
Esp3I	CGTCTC	1 cut(s)	88
FaqI	GGGAC	3 cut(s)	1467, 1500, 2795
FauI	CCCGC	1 cut(s)	197
FauNDI	CATATG	2 cut(s)	1165, 2950
FblI	GTMKAC	4 cut(s)	684, 776, 1281, 2136
Fnu4HI	GCNGC	3 cut(s)	69, 1797, 2712
FriOI	GRGCYC	2 cut(s)	1950, 2358
Fsp4HI	GCNGC	3 cut(s)	69, 1797, 2712
FspBI	CTAG	6 cut(s)	1629, 1757, 1881, 2415, 2576, 2691
GlaI	GCGC	1 cut(s)	496
GluI	GCNGC	3 cut(s)	69, 1797, 2712
GsaI	CCCAGC	2 cut(s)	2733, 2781
GsuI	CTGGAG	1 cut(s)	2918
HaeII	RGCGCY	1 cut(s)	498
HaeIII	GGCC	2 cut(s)	1761, 1961
HapII	CCGG	4 cut(s)	335, 372, 382, 625
HhaI	GCGC	1 cut(s)	497
Hin6I	GCGC	1 cut(s)	495
HinP1I	GCGC	1 cut(s)	495
HincII	GTYRAC	1 cut(s)	2137
HindII	GTYRAC	1 cut(s)	2137
HindIII	AAGCTT	5 cut(s)	211, 503, 719, 1432, 2936
HpaII	CCGG	4 cut(s)	335, 372, 382, 625
HphI	GGTGA	8 cut(s)	66, 89, 589, 977, 1021, 2603, 2737, 3023
Hpy166II	GTNNAC	9 cut(s)	44, 97, 685, 777, 1282, 1850, 2129, 2137, 2629
Hpy188I	TCNGA	9 cut(s)	556, 1381, 1537, 1636, 1998, 2031, 2110, 2268, 2453
Hpy8I	GTNNAC	9 cut(s)	44, 97, 685, 777, 1282, 1850, 2129, 2137, 2629
Hpy99I	CGWCG	2 cut(s)	329, 2138
HpyCH4III	ACNGT	6 cut(s)	1808, 1865, 2026, 2533, 2930, 2973
HpyCH4IV	ACGT	5 cut(s)	279, 313, 888, 2313, 2679
HpyF3I	CTNAG	5 cut(s)	91, 848, 2030, 2267, 2918
HpySE526I	ACGT	5 cut(s)	279, 313, 888, 2313, 2679
HspAI	GCGC	1 cut(s)	495
KpnI	GGTACC	1 cut(s)	797
Kzo9I	GATC	8 cut(s)	639, 1311, 1443, 1682, 2296, 2308, 2443, 2887
LguI	GCTCTTC	1 cut(s)	1524
LmnI	GCTCC	3 cut(s)	384, 2416, 2422
Lsp1109I	GCAGC	2 cut(s)	1783, 2723
LweI	GCATC	8 cut(s)	607, 691, 1243, 1336, 1569, 1668, 2181, 2839
MaeI	CTAG	6 cut(s)	1629, 1757, 1881, 2415, 2576, 2691
MaeII	ACGT	5 cut(s)	279, 313, 888, 2313, 2679
MaeIII	GTNAC	6 cut(s)	1705, 2314, 2564, 2743, 2967, 3011
MalI	GATC	8 cut(s)	641, 1313, 1445, 1684, 2298, 2310, 2445, 2889
MbiI	CCGCTC	2 cut(s)	387, 1336
MboI	GATC	8 cut(s)	639, 1311, 1443, 1682, 2296, 2308, 2443, 2887
MhlI	GDGCHC	4 cut(s)	1950, 2131, 2358, 2427
MlyI	GAGTC	2 cut(s)	179, 2000
MmeI	TCCRAC	5 cut(s)	904, 982, 1048, 1892, 2589
Mph1103I	ATGCAT	2 cut(s)	549, 1165
MroXI	GAANNNNTTC	3 cut(s)	213, 1568, 2370
MslI	CAYNNNNRTG	4 cut(s)	1052, 1164, 2082, 2282
MspA1I	CMGCKG	1 cut(s)	39
MspCI	CTTAAG	1 cut(s)	506
MspI	CCGG	4 cut(s)	335, 372, 382, 625
MspR9I	CCNGG	7 cut(s)	373, 382, 485, 626, 664, 943, 2215
Mva1269I	GAATGC	3 cut(s)	1719, 1825, 1890
MvaI	CCWGG	4 cut(s)	485, 664, 943, 2215
MvnI	CGCG	1 cut(s)	3072
NciI	CCSGG	3 cut(s)	373, 382, 626
NcoI	CCATGG	1 cut(s)	1962
NdeI	CATATG	2 cut(s)	1165, 2950
NdeII	GATC	8 cut(s)	639, 1311, 1443, 1682, 2296, 2308, 2443, 2887
NlaIV	GGNNCC	5 cut(s)	444, 795, 2330, 2412, 2613
NmeAIII	GCCGAG	2 cut(s)	471, 2576
NmuCI	GTSAC	3 cut(s)	2743, 2967, 3011
NsiI	ATGCAT	2 cut(s)	549, 1165
NspI	RCATGY	3 cut(s)	736, 2143, 2653
PagI	TCATGA	1 cut(s)	1446
PciI	ACATGT	2 cut(s)	2139, 2649
PciSI	GCTCTTC	1 cut(s)	1524
PctI	GAATGC	3 cut(s)	1719, 1825, 1890
PdmI	GAANNNNTTC	3 cut(s)	213, 1568, 2370
PfeI	GAWTC	8 cut(s)	551, 818, 1082, 1556, 1768, 2105, 2178, 3074
PflMI	CCANNNNNTGG	1 cut(s)	490
PfoI	TCCNGGA	2 cut(s)	662, 2213
PkrI	GCNGC	3 cut(s)	70, 1798, 2713
PleI	GAGTC	2 cut(s)	179, 2000
PpsI	GAGTC	2 cut(s)	179, 2000
Ppu21I	YACGTR	2 cut(s)	314, 889
PpuMI	RGGWCCY	1 cut(s)	2329
PscI	ACATGT	2 cut(s)	2139, 2649
PshBI	ATTAAT	3 cut(s)	1406, 2225, 2469
PsiI	TTATAA	1 cut(s)	717
Psp124BI	GAGCTC	1 cut(s)	1950
Psp1406I	AACGTT	1 cut(s)	279
Psp5II	RGGWCCY	1 cut(s)	2329
Psp6I	CCWGG	4 cut(s)	483, 662, 941, 2213
PspEI	GGTNACC	1 cut(s)	2743
PspFI	CCCAGC	2 cut(s)	2729, 2777
PspGI	CCWGG	4 cut(s)	483, 662, 941, 2213
PspN4I	GGNNCC	5 cut(s)	444, 795, 2330, 2412, 2613
PspPI	GGNCC	1 cut(s)	2329
PspPPI	RGGWCCY	1 cut(s)	2329
PstI	CTGCAG	1 cut(s)	1732
PstNI	CAGNNNCTG	1 cut(s)	710
RsaI	GTAC	4 cut(s)	377, 795, 887, 2400
RsaNI	GTAC	4 cut(s)	376, 794, 886, 2399
RseI	CAYNNNNRTG	4 cut(s)	1052, 1164, 2082, 2282
SacI	GAGCTC	1 cut(s)	1950
SalI	GTCGAC	1 cut(s)	2135
SapI	GCTCTTC	1 cut(s)	1524
SatI	GCNGC	3 cut(s)	69, 1797, 2712
Sau3AI	GATC	8 cut(s)	639, 1311, 1443, 1682, 2296, 2308, 2443, 2887
Sau96I	GGNCC	1 cut(s)	2329
SchI	GAGTC	2 cut(s)	179, 2000
ScrFI	CCNGG	7 cut(s)	373, 382, 485, 626, 664, 943, 2215
SduI	GDGCHC	4 cut(s)	1950, 2131, 2358, 2427
SfaNI	GCATC	8 cut(s)	607, 691, 1243, 1336, 1569, 1668, 2181, 2839
SfcI	CTRYAG	3 cut(s)	1656, 1728, 1776
SinI	GGWCC	1 cut(s)	2329
SmiMI	CAYNNNNRTG	4 cut(s)	1052, 1164, 2082, 2282
SmlI	CTYRAG	3 cut(s)	246, 506, 1038
SmoI	CTYRAG	3 cut(s)	246, 506, 1038
SnaBI	TACGTA	1 cut(s)	889
SsiI	CCGC	9 cut(s)	37, 69, 116, 190, 223, 387, 611, 1336, 2087
SspI	AATATT	3 cut(s)	1078, 1664, 3046
SspMI	CTAG	6 cut(s)	1629, 1757, 1881, 2415, 2576, 2691
SstI	GAGCTC	1 cut(s)	1950
StyD4I	CCNGG	7 cut(s)	371, 380, 483, 624, 662, 941, 2213
StyI	CCWWGG	3 cut(s)	1956, 1962, 2414
TaaI	ACNGT	6 cut(s)	1808, 1865, 2026, 2533, 2930, 2973
TaiI	ACGT	5 cut(s)	282, 316, 891, 2316, 2682
TaqI	TCGA	7 cut(s)	126, 324, 539, 933, 983, 2136, 2892
TaqII	GACCGA	1 cut(s)	670
TatI	WGTACW	1 cut(s)	2398
TauI	GCSGC	1 cut(s)	71
TfiI	GAWTC	8 cut(s)	551, 818, 1082, 1556, 1768, 2105, 2178, 3074
TscAI	CASTG	4 cut(s)	1117, 2721, 2935, 3028
TseFI	GTSAC	3 cut(s)	2743, 2967, 3011
TseI	GCWGC	2 cut(s)	1796, 2711
Tsp45I	GTSAC	3 cut(s)	2743, 2967, 3011
TspRI	CASTG	4 cut(s)	1117, 2721, 2935, 3028
Van91I	CCANNNNNTGG	1 cut(s)	490
Vha464I	CTTAAG	1 cut(s)	506
VneI	GTGCAC	1 cut(s)	2127
VpaK11BI	GGWCC	1 cut(s)	2329
VspI	ATTAAT	3 cut(s)	1406, 2225, 2469
XapI	RAATTY	9 cut(s)	142, 522, 652, 667, 1115, 1606, 1915, 2218, 2873
XbaI	TCTAGA	1 cut(s)	1880
XceI	RCATGY	3 cut(s)	736, 2143, 2653
XmaJI	CCTAGG	1 cut(s)	2414
XmiI	GTMKAC	4 cut(s)	684, 776, 1281, 2136
XmnI	GAANNNNTTC	3 cut(s)	213, 1568, 2370
XspI	CTAG	6 cut(s)	1629, 1757, 1881, 2415, 2576, 2691
Zsp2I	ATGCAT	2 cut(s)	549, 1165

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.