Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0011231

Physical Location & Seq

Genomic Coordinates Forward (+)

73886903 .. 73890130

Gene Structure 3228 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG529500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3228 bp

ATGTATTGCGAAAACGAAAACATATATGAAGGAAGAAAGCACATCACTCTTCTTCACTACAACACCATTATGTTCCGCTCTTTCTTCATTACAGGACTATCTTCTCCCACTCACAAAGCAAAACTCCGCCGCTCTTCACACCCTGCTTTCACTTCTCTCACCGCCTCACCTCTCCCCTCAAACTCACTTCACCACACCAACCAATCATCTCCGCCCCACATCGAAACCCAAACCCCACATTCCAATGCTACTTACGGCTATCTTCTCAATCGGTTCAAGGACTCCCGTACCTCAAACGAAGCCGAAACCTTTCATTTGCGGATTTTCAAACACGGGTTCACTCAAGATTTGTTCTTGTGCAATACCCTCATCAACGTTTACGTCAAAATCGGTGCTTTGGCTAATGCGCGTAAGCTGTTCGACGAAATGCCTGAGAAGAACTCGGTTACTTGGGCTTGCTTGATTTCGGGCTATGCCCGGAGCGGTGTGCCAGAGGAGGCGTGTGGGCAGTTTAAACGGATGGTCTGTGATGGTTTCGTGCCGAGTGTTTATGCTTTTGGGAGTGTTTTAAGGGCTTGCCAGGAGTTGGGTCCTTGCAAACTTAAGTTTGGGATGCAAGTTCAGGGGTTGTTATCGAAAATGCATGATTCGGATGTGTTGTTAGCTAATGTGCTGATGTCAATGTATGGGAGATGTTTGGGTTCTGCCGATGATGCTTACCGGGCTTTTTGTGAGATCAAGTTTAGAAATCTAGTGTCTTGGAATTCTATTATTTCGGTTTACTGCCAAAGAGGGGATGCAGTGTTTGCTTATAAGCTTTTCTCAAACATGCAGCAGGATGGTGTGGATTTTGATTTGCAACCGAATGAGTATACTTTTGGTAGCTTGGTACCTGCCTGTTGTGCTTTGGCTGATTCTGGTTTGGTTTTGCTTCAGCAAATGCTGAGCAGGGTCAGCAAAGCTGGATTTTTACAGGATTTGTATGTAGGAAGTGCTTTGGTTAGTGGATTTGCGAGGGTTGGATTAGTCGATTACGCTAGGAAGATTTTTGAGCAGATGGGTGAGAGGAATGTAGTTTCGATGAATGGGTTGATGGTTGGACTGGTGAGGCAGAAGCGAGGAGAAGAAGCCACTCAAGTTTTCATGGAGATGAAGGACTTGGTTGGAATAAATATTGATTCGCTTGTGGTTCTTTTAAGTTCATTCACTGAATTTTCTGCTTTAGAGGAAGGGAAAAGAAAGGGAAGAGAAGTTCATGCATATGTAATTGCAACTGGCTTGATTCACAGCAAGGTTGCAATAGAGAATGGGCTTGTAAATATGTATGCCAAATGTGGTGCTATTGATGATGCTTGTTCAGTTTTTAGGCTCATGGTAGACAAAGACTTAATCTCATGGAACTCTCTGATCTCTGGTCTCGACCAGAACAAGTGTTTTGAAGATGCAGTCATGAAGTTCTCTGAAATGAGAACATCTGAGTTGACACCTTCAATTTTCACATTAATTAGTGCTTTGAGTTCATGTGCAAGCTTGGAGTGGATCATGATGGGACAGCAAATACATTGTGAAGCACTTAAATTGGGACTTGATTTGGATGTTTCGGTTTCAAATGCTCTTCTTGCATTGTATTCTGACACAGGGTGTCTTAATGAATCCCGGAATGTTTTCTTCTTGATGCAGGAATATGACCAAGTGTCATGGAATTCCATAGTTGGGGCTTTGGCTAGTTCAGAGGCTTCAGTTTTTGAAGCTGTAGAATATTTATTGGAAATGATGCGATCTGGATGGGAACTTAATAGAGTAACATTTATTAGCATTCTTTCTGCAGTTTCATCTCTCTCACTTCACGAGCTAGGCCAGCAGATTCATGCTGTAGTTTTAAAATACAATGCTATGAAGGACTGTGCTATTGAGAATGCACTTATTGCTTGCTATGGAAAGTGTGGACAGATAGATGACTGTGAGAAGATATTTTCTAGAATGCCTGAAAGAAGGGATGAAGTAAGTTGGAATTCCATGATTTCTGGATACATACATAATGAGCTCTTGCCCAGGGCCATGGATTTGGTCTGGTTTATGATTGAGAGGGGTCAGAAATTAGACTCTTTTACTTTTGCTACTGTTCTGAGTGCTTGTGCTTCAGTTGCAACATTAGAGCGTGGGATGGAAGTTCATGCTTGTGCGGTAAGAGCTTATTTGGAATCTGATGTTGTAGTTGGGAGTGCACTCGTCGACATGTATTCAAAGTGTGGAAGGATTGATTATGCTTCAAGATTCTTTGAGTTGATGCCAGTAAGGAATGTGTATTCCTGGAATTCATTAATATCAGGATATGCTCGCAATGGAGATGGACAGAAAGCTCTGAGGCTTTTCACACAAATGAAGCAACAAGATCAGCCACCAGATCACGTTACCTTTGTTGGGGTCCTATCAGCTTGTAGCCACGCAGGGCTTGTGGATGAAGGGTTTCAGCATTTCAATTCCATGAGTAAAGTACATGGACTGGCTCCTAGGATGGAGCACTTTTCATGTATGGTTGATCTTCTTGGAAGGGCAGGAAAAATTAATATGATAGAGGACTTCATAAAAAAAATGCCGTTGAAGCCTAATGTGCTTATTTGGAGGACAGTTTTAGGGGCTTGTTGCCGAGGCAATGGTCGTAACACAGAGCTAGGCAGGATGGCTGGTGAAATGCTATTGGAGTTGGAACCCCAAAATGCTGTGAACTATGTGCTTCTTGCTAACATGTATGCTTCTGGTGGGAAGTGGGATGACGTGGCAAAGGCTAGGATGACAATGAGAAAGGCAGCAGTGAAGAAGGAAGCTGGGTGCAGTTGGGTCACCATGAAAGATGGGGTTCATGTTTTTGTAGCTGGGGACAAATTACACCCAGAGAAAGATTTGATATATGAAAAACTGAAGGAACTTAACGAGAAAATGAGGAATGCTGGATATGTGCCTGAAACAAAATTTGCACTATACGATCTCGAACTGGAGAACAAGGAAGAACTCCTGAGTTATCACAGTGAAAAGCTTGCTGTTGCATATGTTCTTACTCGCCAGTCACAGTTGCCTATAAGGATTATGAAAAACCTTCGGGTTTGTGGTGACTGCCACACTGCCTTCAAATACATATCAAATATTGTTGGCCGGCAAATAGTATTACGCGATTCAAACAGATTTCATCACTTTCAAGATGGCAAGTGTTCTTGCGGGGATTTCTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

1075

Amino Acids

120.46

Weight (kDa)

6.66

Isoelectric Point (pI)

41.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	146 - 193	1.6e-07	PPR repeat family
PPR_2	PF13041	250 - 301	1.4e-10	PPR repeat family
PPR	PF01535	252 - 282	2.7e-06	PPR repeat
PPR_2	PF13041	666 - 713	4.8e-08	PPR repeat family
PPR_2	PF13041	766 - 814	1.3e-12	PPR repeat family
PPR_1	PF12854	766 - 795	8.4e-06	PPR repeat
PPR	PF01535	769 - 796	5.7e-08	PPR repeat
E_motif	PF20431	888 - 947	2.1e-19	E motif
DYW_deaminase	PF14432	984 - 1075	6.6e-40	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011231)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09950 AT5G09950 AT5G09950
fragaria_vesca	FvH4_6g40670
malus_domestica	MD09G1126300.v1.1
prunus_persica	Prupe.3G198300_v2.0.a1
pyrus_communis	pycom09g05060
rosa_chinensis	RchiOBHm_Chr2g0156061 RchiOBHm_Chr2g0156111
rosa_laevigata	RLG00000020884 RLG00000020888
rosa_multiflora	Rmu_sc0000260.1_g000021 Rmu_sc0011922.1_g000009
rosa_roxburghii	Rroxscaffold_2G00093230
rosa_rugosa	Rorug02G0451500
rosa_samantha	Rh2AG518400 Rh2BG529500 Rh2CG503100 Rh2DG538700
rosa_wichuraiana	Rw2G042680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	813
Acc36I	ACCTGC	1 cut(s)	901
Acc65I	GGTACC	1 cut(s)	889
AccB1I	GGYRCC	1 cut(s)	889
AccB7I	CCANNNNNTGG	1 cut(s)	586
AccBSI	CCGCTC	3 cut(s)	78, 132, 483
AccI	GTMKAC	3 cut(s)	872, 1377, 2232
AccII	CGCG	2 cut(s)	409, 3168
AciI	CCGC	9 cut(s)	76, 127, 130, 162, 212, 319, 483, 2183, 3213
AclI	AACGTT	1 cut(s)	375
AclWI	GGATC	1 cut(s)	1547
AcoI	YGGCCR	1 cut(s)	3148
AcsI	RAATTY	6 cut(s)	763, 1211, 1702, 2011, 2314, 2969
AcuI	CTGAAG	4 cut(s)	917, 1722, 2124, 2939
AdeI	CACNNNGTG	1 cut(s)	1641
AfaI	GTAC	3 cut(s)	289, 891, 2496
AfiI	CCNNNNNNNGG	4 cut(s)	586, 793, 1713, 2421
AflII	CTTAAG	1 cut(s)	602
AflIII	ACRYGT	2 cut(s)	2235, 2745
AhdI	GACNNNNNGTC	1 cut(s)	1693
AjiI	CACGTC	1 cut(s)	2776
AjnI	CCWGG	3 cut(s)	579, 2051, 2309
AloI	GAACNNNNNNTCC	2 cut(s)	1236, 1268
Alw21I	GWGCWC	3 cut(s)	2046, 2227, 2523
Alw26I	GTCTC	1 cut(s)	1421
Alw44I	GTGCAC	1 cut(s)	2223
AlwI	GGATC	1 cut(s)	1547
AoxI	GGCC	3 cut(s)	1855, 2055, 3148
ApaLI	GTGCAC	1 cut(s)	2223
ApeKI	GCWGC	2 cut(s)	832, 2807
ApoI	RAATTY	6 cut(s)	763, 1211, 1702, 2011, 2314, 2969
AseI	ATTAAT	3 cut(s)	1502, 2321, 2565
Asp700I	GAANNNNTTC	3 cut(s)	309, 1664, 2466
Asp718I	GGTACC	1 cut(s)	889
AspA2I	CCTAGG	1 cut(s)	2510
AspLEI	GCGC	1 cut(s)	409
AspS9I	GGNCC	3 cut(s)	590, 2055, 2425
AsuC2I	CCSGG	3 cut(s)	478, 722, 1657
AsuHPI	GGTGA	8 cut(s)	151, 159, 182, 1073, 1117, 2699, 2833, 3119
AvaII	GGWCC	2 cut(s)	590, 2425
AvrII	CCTAGG	1 cut(s)	2510
BaeGI	GKGCMC	1 cut(s)	2227
BaeI	ACNNNNGTAYC	2 cut(s)	271, 304
BanI	GGYRCC	1 cut(s)	889
BanII	GRGCYC	1 cut(s)	2046
BauI	CACGAG	1 cut(s)	1847
Bbv12I	GWGCWC	3 cut(s)	2046, 2227, 2523
BbvI	GCAGC	2 cut(s)	844, 2819
BceAI	ACGGC	2 cut(s)	271, 2581
BciT130I	CCWGG	3 cut(s)	581, 2053, 2311
BciVI	GTATCC	1 cut(s)	2021
BcnI	CCSGG	3 cut(s)	478, 722, 1657
BcoDI	GTCTC	1 cut(s)	1421
BfaI	CTAG	8 cut(s)	752, 1038, 1725, 1853, 1977, 2511, 2672, 2787
BfmI	CTRYAG	3 cut(s)	1752, 1824, 1872
BfrI	CTTAAG	1 cut(s)	602
BfuAI	ACCTGC	1 cut(s)	901
BfuI	GTATCC	1 cut(s)	2021
BisI	GCNGC	3 cut(s)	130, 833, 2808
BlnI	CCTAGG	1 cut(s)	2510
BlpI	GCTNAGC	1 cut(s)	944
BlsI	GCNGC	3 cut(s)	131, 834, 2809
Bme1390I	CCNGG	6 cut(s)	478, 581, 722, 1657, 2053, 2311
Bme18I	GGWCC	2 cut(s)	590, 2425
BmeRI	GACNNNNNGTC	1 cut(s)	1693
BmgBI	CACGTC	1 cut(s)	2776
BmgT120I	GGNCC	3 cut(s)	590, 2055, 2425
BmiI	GGNNCC	5 cut(s)	591, 891, 2426, 2508, 2709
BmrFI	CCNGG	6 cut(s)	478, 581, 722, 1657, 2053, 2311
BmsI	GCATC	8 cut(s)	603, 703, 787, 1339, 1432, 1665, 1764, 2277
BplI	GAGNNNNNCTC	2 cut(s)	425, 457
BpmI	CTGGAG	1 cut(s)	3014
Bpu1102I	GCTNAGC	1 cut(s)	944
BpuEI	CTTGAG	2 cut(s)	327, 1119
BpuMI	CCSGG	3 cut(s)	478, 722, 1657
BsaI	GGTCTC	1 cut(s)	1421
BsaJI	CCNNGG	5 cut(s)	2051, 2052, 2058, 2510, 2647
Bsc4I	CCNNNNNNNGG	4 cut(s)	586, 793, 1713, 2421
Bse118I	RCCGGY	1 cut(s)	3150
Bse1I	ACTGG	6 cut(s)	1107, 1279, 2291, 2508, 2997, 3061
Bse3DI	GCAATG	2 cut(s)	2347, 2659
BseBI	CCWGG	3 cut(s)	581, 2053, 2311
BseDI	CCNNGG	5 cut(s)	2051, 2052, 2058, 2510, 2647
BseLI	CCNNNNNNNGG	4 cut(s)	586, 793, 1713, 2421
BseMI	GCAATG	2 cut(s)	2347, 2659
BseMII	CTCAG	6 cut(s)	423, 935, 1467, 2117, 2354, 3005
BseNI	ACTGG	6 cut(s)	1107, 1279, 2291, 2508, 2997, 3061
BseRI	GAGGAG	2 cut(s)	509, 1134
BseSI	GKGCMC	1 cut(s)	2227
BseXI	GCAGC	2 cut(s)	844, 2819
BseYI	CCCAGC	2 cut(s)	2825, 2873
BsgI	GTGCAG	1 cut(s)	2851
Bsh1236I	CGCG	2 cut(s)	409, 3168
BshFI	GGCC	3 cut(s)	1857, 2057, 3150
BshNI	GGYRCC	1 cut(s)	889
BsiHKAI	GWGCWC	3 cut(s)	2046, 2227, 2523
BsiSI	CCGG	4 cut(s)	478, 721, 1657, 3151
BslFI	GGGAC	3 cut(s)	1563, 1596, 2891
BslI	CCNNNNNNNGG	4 cut(s)	586, 793, 1713, 2421
BsmAI	GTCTC	1 cut(s)	1421
BsmFI	GGGAC	3 cut(s)	1563, 1596, 2891
BsmI	GAATGC	4 cut(s)	1815, 1921, 1986, 2950
BsnI	GGCC	3 cut(s)	1857, 2057, 3150
Bso31I	GGTCTC	1 cut(s)	1421
Bsp1286I	GDGCHC	3 cut(s)	2046, 2227, 2523
Bsp143I	GATC	8 cut(s)	735, 1407, 1539, 1778, 2392, 2404, 2539, 2983
Bsp1720I	GCTNAGC	1 cut(s)	944
Bsp19I	CCATGG	1 cut(s)	2058
BspACI	CCGC	9 cut(s)	76, 127, 130, 162, 212, 319, 483, 2183, 3213
BspANI	GGCC	3 cut(s)	1857, 2057, 3150
BspCNI	CTCAG	6 cut(s)	424, 936, 1468, 2118, 2355, 3006
BspFNI	CGCG	2 cut(s)	409, 3168
BspHI	TCATGA	2 cut(s)	1449, 1542
BspLI	GGNNCC	5 cut(s)	591, 891, 2426, 2508, 2709
BspMAI	CTGCAG	1 cut(s)	1828
BspMI	ACCTGC	1 cut(s)	901
BspPI	GGATC	1 cut(s)	1547
BspQI	GCTCTTC	2 cut(s)	139, 1620
BspT107I	GGYRCC	1 cut(s)	889
BspTI	CTTAAG	1 cut(s)	602
BspTNI	GGTCTC	1 cut(s)	1421
BsrBI	CCGCTC	3 cut(s)	78, 132, 483
BsrDI	GCAATG	2 cut(s)	2347, 2659
BsrFI	RCCGGY	1 cut(s)	3150
BsrI	ACTGG	6 cut(s)	1107, 1279, 2291, 2508, 2997, 3061
BssAI	RCCGGY	1 cut(s)	3150
BssECI	CCNNGG	5 cut(s)	2051, 2052, 2058, 2510, 2647
BssMI	GATC	8 cut(s)	735, 1407, 1539, 1778, 2392, 2404, 2539, 2983
BssNAI	GTATAC	1 cut(s)	873
BssSI	CACGAG	1 cut(s)	1847
BssT1I	CCWWGG	2 cut(s)	2058, 2510
Bst1107I	GTATAC	1 cut(s)	873
Bst2BI	CACGAG	1 cut(s)	1847
Bst2UI	CCWGG	3 cut(s)	581, 2053, 2311
Bst4CI	ACNGT	6 cut(s)	1904, 1961, 2122, 2629, 3026, 3069
Bst6I	CTCTTC	4 cut(s)	54, 139, 1240, 1620
BstAFI	CTTAAG	1 cut(s)	602
BstAPI	GCANNNNNTGC	2 cut(s)	806, 1925
BstC8I	GCNNGC	8 cut(s)	457, 577, 1528, 1859, 1930, 2338, 3036, 3152
BstDEI	CTNAG	6 cut(s)	432, 944, 1476, 2126, 2363, 3014
BstDSI	CCRYGG	1 cut(s)	2058
BstEII	GGTNACC	1 cut(s)	2839
BstFNI	CGCG	2 cut(s)	409, 3168
BstHHI	GCGC	1 cut(s)	409
BstKTI	GATC	8 cut(s)	738, 1410, 1542, 1781, 2395, 2407, 2542, 2986
BstMAI	GTCTC	1 cut(s)	1421
BstMBI	GATC	8 cut(s)	735, 1407, 1539, 1778, 2392, 2404, 2539, 2983
BstNI	CCWGG	3 cut(s)	581, 2053, 2311
BstNSI	RCATGY	3 cut(s)	832, 2239, 2749
BstPI	GGTNACC	1 cut(s)	2839
BstSCI	CCNGG	6 cut(s)	476, 579, 720, 1655, 2051, 2309
BstSFI	CTRYAG	3 cut(s)	1752, 1824, 1872
BstSLI	GKGCMC	1 cut(s)	2227
BstUI	CGCG	2 cut(s)	409, 3168
BstV1I	GCAGC	2 cut(s)	844, 2819
BstXI	CCANNNNNNTGG	2 cut(s)	2059, 2065
BstZ17I	GTATAC	1 cut(s)	873
BsuI	GTATCC	1 cut(s)	2021
BsuRI	GGCC	3 cut(s)	1857, 2057, 3150
BtgI	CCRYGG	1 cut(s)	2058
BtrI	CACGTC	1 cut(s)	2776
BtsI	GCAGTG	3 cut(s)	807, 2817, 3117
BtsIMutI	CAGTG	5 cut(s)	807, 1206, 2817, 3031, 3117
BveI	ACCTGC	1 cut(s)	901
Cac8I	GCNNGC	8 cut(s)	457, 577, 1528, 1859, 1930, 2338, 3036, 3152
CciI	TCATGA	2 cut(s)	1449, 1542
CfoI	GCGC	1 cut(s)	409
Cfr10I	RCCGGY	1 cut(s)	3150
Cfr13I	GGNCC	3 cut(s)	590, 2055, 2425
Csp6I	GTAC	3 cut(s)	288, 890, 2495
CviQI	GTAC	3 cut(s)	288, 890, 2495
DdeI	CTNAG	6 cut(s)	432, 944, 1476, 2126, 2363, 3014
DpnI	GATC	8 cut(s)	737, 1409, 1541, 1780, 2394, 2406, 2541, 2985
DpnII	GATC	8 cut(s)	735, 1407, 1539, 1778, 2392, 2404, 2539, 2983
DraI	TTTAAA	2 cut(s)	514, 1881
DraIII	CACNNNGTG	1 cut(s)	1641
DriI	GACNNNNNGTC	1 cut(s)	1693
EaeI	YGGCCR	1 cut(s)	3148
Eam1104I	CTCTTC	4 cut(s)	54, 139, 1240, 1620
Eam1105I	GACNNNNNGTC	1 cut(s)	1693
EarI	CTCTTC	4 cut(s)	54, 139, 1240, 1620
EciI	GGCGGA	2 cut(s)	116, 201
Ecl136II	GAGCTC	1 cut(s)	2044
Eco130I	CCWWGG	2 cut(s)	2058, 2510
Eco24I	GRGCYC	1 cut(s)	2046
Eco31I	GGTCTC	1 cut(s)	1421
Eco47I	GGWCC	2 cut(s)	590, 2425
Eco53kI	GAGCTC	1 cut(s)	2044
Eco57I	CTGAAG	4 cut(s)	917, 1722, 2124, 2939
Eco91I	GGTNACC	1 cut(s)	2839
EcoICRI	GAGCTC	1 cut(s)	2044
EcoO109I	RGGNCCY	2 cut(s)	590, 2425
EcoO65I	GGTNACC	1 cut(s)	2839
EcoRI	GAATTC	4 cut(s)	763, 1702, 2011, 2314
EcoRII	CCWGG	3 cut(s)	579, 2051, 2309
EcoT14I	CCWWGG	2 cut(s)	2058, 2510
EcoT22I	ATGCAT	2 cut(s)	645, 1261
EcoT38I	GRGCYC	1 cut(s)	2046
ErhI	CCWWGG	2 cut(s)	2058, 2510
FaqI	GGGAC	3 cut(s)	1563, 1596, 2891
FauI	CCCGC	1 cut(s)	3206
FauNDI	CATATG	2 cut(s)	1261, 3046
FblI	GTMKAC	3 cut(s)	872, 1377, 2232
Fnu4HI	GCNGC	3 cut(s)	130, 833, 2808
FriOI	GRGCYC	1 cut(s)	2046
Fsp4HI	GCNGC	3 cut(s)	130, 833, 2808
FspBI	CTAG	8 cut(s)	752, 1038, 1725, 1853, 1977, 2511, 2672, 2787
GlaI	GCGC	1 cut(s)	408
GluI	GCNGC	3 cut(s)	130, 833, 2808
GsaI	CCCAGC	2 cut(s)	2829, 2877
GsuI	CTGGAG	1 cut(s)	3014
HaeIII	GGCC	3 cut(s)	1857, 2057, 3150
HapII	CCGG	4 cut(s)	478, 721, 1657, 3151
HhaI	GCGC	1 cut(s)	409
Hin6I	GCGC	1 cut(s)	407
HinP1I	GCGC	1 cut(s)	407
HincII	GTYRAC	2 cut(s)	1482, 2233
HindII	GTYRAC	2 cut(s)	1482, 2233
HindIII	AAGCTT	3 cut(s)	815, 1528, 3032
HpaII	CCGG	4 cut(s)	478, 721, 1657, 3151
HphI	GGTGA	8 cut(s)	151, 159, 182, 1073, 1117, 2699, 2833, 3119
Hpy99I	CGWCG	2 cut(s)	425, 2234
HpyCH4III	ACNGT	6 cut(s)	1904, 1961, 2122, 2629, 3026, 3069
HpyCH4IV	ACGT	4 cut(s)	375, 381, 2409, 2775
HpyF3I	CTNAG	6 cut(s)	432, 944, 1476, 2126, 2363, 3014
HpySE526I	ACGT	4 cut(s)	375, 381, 2409, 2775
HspAI	GCGC	1 cut(s)	407
KpnI	GGTACC	1 cut(s)	893
KroI	GCCGGC	1 cut(s)	3150
KroNI	GCCGGC	1 cut(s)	3152
Kzo9I	GATC	8 cut(s)	735, 1407, 1539, 1778, 2392, 2404, 2539, 2983
LguI	GCTCTTC	2 cut(s)	139, 1620
LmnI	GCTCC	3 cut(s)	480, 2512, 2518
Lsp1109I	GCAGC	2 cut(s)	844, 2819
LweI	GCATC	8 cut(s)	603, 703, 787, 1339, 1432, 1665, 1764, 2277
MaeI	CTAG	8 cut(s)	752, 1038, 1725, 1853, 1977, 2511, 2672, 2787
MaeII	ACGT	4 cut(s)	375, 381, 2409, 2775
MaeIII	GTNAC	7 cut(s)	445, 1801, 2410, 2660, 2839, 3063, 3107
MalI	GATC	8 cut(s)	737, 1409, 1541, 1780, 2394, 2406, 2541, 2985
MbiI	CCGCTC	3 cut(s)	78, 132, 483
MboI	GATC	8 cut(s)	735, 1407, 1539, 1778, 2392, 2404, 2539, 2983
MhlI	GDGCHC	3 cut(s)	2046, 2227, 2523
MlyI	GAGTC	2 cut(s)	275, 2096
MmeI	TCCRAC	5 cut(s)	1000, 1078, 1144, 1988, 2685
Mph1103I	ATGCAT	2 cut(s)	645, 1261
MroNI	GCCGGC	1 cut(s)	3150
MroXI	GAANNNNTTC	3 cut(s)	309, 1664, 2466
MslI	CAYNNNNRTG	6 cut(s)	68, 243, 1148, 1260, 2178, 2378
MspCI	CTTAAG	1 cut(s)	602
MspI	CCGG	4 cut(s)	478, 721, 1657, 3151
MspR9I	CCNGG	6 cut(s)	478, 581, 722, 1657, 2053, 2311
MssI	GTTTAAAC	1 cut(s)	514
Mva1269I	GAATGC	4 cut(s)	1815, 1921, 1986, 2950
MvaI	CCWGG	3 cut(s)	581, 2053, 2311
MvnI	CGCG	2 cut(s)	409, 3168
NaeI	GCCGGC	1 cut(s)	3152
NciI	CCSGG	3 cut(s)	478, 722, 1657
NcoI	CCATGG	1 cut(s)	2058
NdeI	CATATG	2 cut(s)	1261, 3046
NdeII	GATC	8 cut(s)	735, 1407, 1539, 1778, 2392, 2404, 2539, 2983
NgoMIV	GCCGGC	1 cut(s)	3150
NlaIV	GGNNCC	5 cut(s)	591, 891, 2426, 2508, 2709
NmeAIII	GCCGAG	2 cut(s)	567, 2672
NmuCI	GTSAC	3 cut(s)	2839, 3063, 3107
NsiI	ATGCAT	2 cut(s)	645, 1261
NspI	RCATGY	3 cut(s)	832, 2239, 2749
PagI	TCATGA	2 cut(s)	1449, 1542
PasI	CCCWGGG	1 cut(s)	2052
PciI	ACATGT	2 cut(s)	2235, 2745
PciSI	GCTCTTC	2 cut(s)	139, 1620
PctI	GAATGC	4 cut(s)	1815, 1921, 1986, 2950
PdiI	GCCGGC	1 cut(s)	3152
PdmI	GAANNNNTTC	3 cut(s)	309, 1664, 2466
PfeI	GAWTC	9 cut(s)	647, 914, 1178, 1282, 1652, 1864, 2201, 2274, 3170
PflMI	CCANNNNNTGG	1 cut(s)	586
PfoI	TCCNGGA	2 cut(s)	1655, 2309
PkrI	GCNGC	3 cut(s)	131, 834, 2809
PleI	GAGTC	2 cut(s)	275, 2096
PmeI	GTTTAAAC	1 cut(s)	514
PpsI	GAGTC	2 cut(s)	275, 2096
PpuMI	RGGWCCY	2 cut(s)	590, 2425
PscI	ACATGT	2 cut(s)	2235, 2745
PshBI	ATTAAT	3 cut(s)	1502, 2321, 2565
PsiI	TTATAA	1 cut(s)	813
Psp124BI	GAGCTC	1 cut(s)	2046
Psp1406I	AACGTT	1 cut(s)	375
Psp5II	RGGWCCY	2 cut(s)	590, 2425
Psp6I	CCWGG	3 cut(s)	579, 2051, 2309
PspEI	GGTNACC	1 cut(s)	2839
PspFI	CCCAGC	2 cut(s)	2825, 2873
PspGI	CCWGG	3 cut(s)	579, 2051, 2309
PspN4I	GGNNCC	5 cut(s)	591, 891, 2426, 2508, 2709
PspPI	GGNCC	3 cut(s)	590, 2055, 2425
PspPPI	RGGWCCY	2 cut(s)	590, 2425
PstI	CTGCAG	1 cut(s)	1828
RsaI	GTAC	3 cut(s)	289, 891, 2496
RsaNI	GTAC	3 cut(s)	288, 890, 2495
RseI	CAYNNNNRTG	6 cut(s)	68, 243, 1148, 1260, 2178, 2378
SacI	GAGCTC	1 cut(s)	2046
SalI	GTCGAC	1 cut(s)	2231
SapI	GCTCTTC	2 cut(s)	139, 1620
SatI	GCNGC	3 cut(s)	130, 833, 2808
Sau3AI	GATC	8 cut(s)	735, 1407, 1539, 1778, 2392, 2404, 2539, 2983
Sau96I	GGNCC	3 cut(s)	590, 2055, 2425
SchI	GAGTC	2 cut(s)	275, 2096
ScrFI	CCNGG	6 cut(s)	478, 581, 722, 1657, 2053, 2311
SduI	GDGCHC	3 cut(s)	2046, 2227, 2523
SfaNI	GCATC	8 cut(s)	603, 703, 787, 1339, 1432, 1665, 1764, 2277
SfcI	CTRYAG	3 cut(s)	1752, 1824, 1872
SinI	GGWCC	2 cut(s)	590, 2425
SmiMI	CAYNNNNRTG	6 cut(s)	68, 243, 1148, 1260, 2178, 2378
SmlI	CTYRAG	3 cut(s)	342, 602, 1134
SmoI	CTYRAG	3 cut(s)	342, 602, 1134
SsiI	CCGC	9 cut(s)	76, 127, 130, 162, 212, 319, 483, 2183, 3213
SspI	AATATT	3 cut(s)	1174, 1760, 3142
SspMI	CTAG	8 cut(s)	752, 1038, 1725, 1853, 1977, 2511, 2672, 2787
SstI	GAGCTC	1 cut(s)	2046
StyD4I	CCNGG	6 cut(s)	476, 579, 720, 1655, 2051, 2309
StyI	CCWWGG	2 cut(s)	2058, 2510
TaaI	ACNGT	6 cut(s)	1904, 1961, 2122, 2629, 3026, 3069
TaiI	ACGT	4 cut(s)	378, 384, 2412, 2778
TaqI	TCGA	8 cut(s)	222, 420, 635, 1029, 1079, 1419, 2232, 2988
TatI	WGTACW	1 cut(s)	2494
TauI	GCSGC	1 cut(s)	132
TfiI	GAWTC	9 cut(s)	647, 914, 1178, 1282, 1652, 1864, 2201, 2274, 3170
TscAI	CASTG	5 cut(s)	807, 1213, 2817, 3031, 3124
TseFI	GTSAC	3 cut(s)	2839, 3063, 3107
TseI	GCWGC	2 cut(s)	832, 2807
Tsp45I	GTSAC	3 cut(s)	2839, 3063, 3107
TspGWI	ACGGA	1 cut(s)	532
TspRI	CASTG	5 cut(s)	807, 1213, 2817, 3031, 3124
Van91I	CCANNNNNTGG	1 cut(s)	586
Vha464I	CTTAAG	1 cut(s)	602
VneI	GTGCAC	1 cut(s)	2223
VpaK11BI	GGWCC	2 cut(s)	590, 2425
VspI	ATTAAT	3 cut(s)	1502, 2321, 2565
XapI	RAATTY	6 cut(s)	763, 1211, 1702, 2011, 2314, 2969
XbaI	TCTAGA	1 cut(s)	1976
XceI	RCATGY	3 cut(s)	832, 2239, 2749
XmaJI	CCTAGG	1 cut(s)	2510
XmiI	GTMKAC	3 cut(s)	872, 1377, 2232
XmnI	GAANNNNTTC	3 cut(s)	309, 1664, 2466
XspI	CTAG	8 cut(s)	752, 1038, 1725, 1853, 1977, 2511, 2672, 2787
Zsp2I	ATGCAT	2 cut(s)	645, 1261

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.