pycom09g15440

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0010038
Physical Location & Seq
Reverse (-)
15305882 .. 15310613
4732 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1290 bp
ATGGCCGCTCTGAAGCTCCTCCTCTCATCCGCTCGCCGTCACTGCCTCTCAAACCCTCACTCCCGTCTCCACCCATCTCCATTTCTAAGCTACCACAGATCCCTAAGCTCCGAATCCGACCCAAACCCGAATCAGAACCCTCCGCAGCCTCCTAGACGCCAACCCGTGCCGGTCGAACCCGTTTCTTACACTCTCAAACCCAAAGAGCAATCCCTACCCGATCCAGATGCGACTCCCCAAGCAGCACCAACATTTCCGCCGCCTCCACAAATCCCCGGTGACTCGCCCAACTCATCGAGTCAGGACACTCGCGTCACCTGGACCCGTGAAGAGGCGCGTTTCATCAGGGACGTTCCATCTATATCTCCGGTATCGTACCCGGTGAAAGTTGCTCCCTTACCGGAAGATAGGGTCGCAGAGGAGAGCGGAGGTGGCGAAGGCGGAGCTGAAAAGGATGTGGATGGCGTGAAGGACTTGGAGAGAGAGAAGAGGAGGATCGAGGCCGATGGTCGGATGAGGAGGAGGCTTTTTAGGGTTCCTGAGGAGGAGGTGGTGGTTCCTTTTCCCGCGTTGATTAAGGTTGAGAAGAAGGAGAGCAAATCTATTCTCGAGGTCATGGACGCAATTCGCCAAGTCAAGGCTAATGCCCAGTGCAAATTTGATGAAACTGTTGAAGCTCATGTACAATTGGGTATTGATGCAAAGCGAAATGCGGTTCGTGGTAACATGACTTTGCCGCATGGTAGTGGGAAGGTTGTCAGGGTTGCTTTCTTTGCTGAAGGCTCAGATGCAGAAGAAGCTAGAGCTGCAGGAGCAGATATTGTTGGCGGTGTTGAACTGGTTGAGGAAATTGCAAGCAGTCATAAGCTCAATGTTGACAAGTGTTTTGCTACAAATGAAATGATTATGCGCCTGGCAAAGATTGCAAGAATTCTTAGAGAGCGTGGTTTGATGCCTGACCGTAAACTAGGTACTGTCACCAATGACATTACTGGAGCACTGCAGAAGGTAAGACAAGGTCATATTGAGTATAGAATGGACAGAACATCAATTGTACATGTGGGACTTGGAAAGGTGAGCTTCACGGAGGAGTATCTGCAAGAGAACATTGGTGCATTTATGAGTTCTCTTTTGCTGGCAAAGCCTACAGGTTTAAAAAAGTCTTCCAAATATGCGGGGTATATTAACTCGTTCCATATATGTAGCACGATGGGTCCAGGATTCCCGGTTTCAATACAGTCATTGTCCAAAGCTGCAGATCACTACAACAAAGTGCACCTCAAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L1

Protein Analysis

430

Amino Acids

47.24

Weight (kDa)

8.97

Isoelectric Point (pI)

58.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L1 PF00687 213 - 406 8.7e-47 Ribosomal protein L1p/L10e family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 311
AccBSI CCGCTC 3 cut(s) 8, 32, 426
AccII CGCG 3 cut(s) 312, 337, 569
AclWI GGATC 3 cut(s) 93, 215, 503
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 656, 930
AcuI CTGAAG 2 cut(s) 32, 798
AcyI GRCGYC 1 cut(s) 157
AfaI GTAC 4 cut(s) 377, 684, 973, 1056
AfiI CCNNNNNNNGG 3 cut(s) 331, 510, 637
AflIII ACRYGT 1 cut(s) 1057
AgsI TTSAA 3 cut(s) 674, 836, 1233
AjnI CCWGG 3 cut(s) 317, 912, 1216
Alw21I GWGCWC 2 cut(s) 1000, 1278
Alw26I GTCTC 1 cut(s) 71
Alw44I GTGCAC 1 cut(s) 1274
AlwI GGATC 3 cut(s) 93, 215, 503
Ama87I CYCGRG 1 cut(s) 608
AoxI GGCC 2 cut(s) 3, 501
ApaLI GTGCAC 1 cut(s) 1274
ApeKI GCWGC 4 cut(s) 145, 242, 806, 1253
ApoI RAATTY 2 cut(s) 656, 930
ArsI GACNNNNNNTTYG 2 cut(s) 869, 901
AspLEI GCGC 2 cut(s) 337, 912
AspS9I GGNCC 2 cut(s) 321, 1214
AsuC2I CCSGG 3 cut(s) 276, 380, 1226
AsuHPI GGTGA 5 cut(s) 290, 307, 394, 970, 1087
AvaI CYCGRG 1 cut(s) 608
AvaII GGWCC 2 cut(s) 321, 1214
AxyI CCTNAGG 1 cut(s) 540
BaeGI GKGCMC 1 cut(s) 1278
BarI GAAGNNNNNNTAC 2 cut(s) 666, 698
BbsI GAAGAC 1 cut(s) 1155
Bbv12I GWGCWC 2 cut(s) 1000, 1278
BbvI GCAGC 4 cut(s) 157, 254, 793, 1240
BccI CCATC 5 cut(s) 82, 364, 455, 500, 1204
BceAI ACGGC 1 cut(s) 21
BcgI CGANNNNNNTGC 2 cut(s) 209, 243
BciT130I CCWGG 3 cut(s) 319, 914, 1218
BcnI CCSGG 3 cut(s) 276, 380, 1226
BcoDI GTCTC 1 cut(s) 71
BfaI CTAG 3 cut(s) 153, 801, 968
BfmI CTRYAG 4 cut(s) 807, 1001, 1146, 1254
BisI GCNGC 7 cut(s) 6, 146, 243, 260, 737, 807, 1254
BlsI GCNGC 7 cut(s) 7, 147, 244, 261, 738, 808, 1255
Bme1390I CCNGG 6 cut(s) 276, 319, 380, 914, 1218, 1226
Bme18I GGWCC 2 cut(s) 321, 1214
BmeT110I CYCGRG 1 cut(s) 608
BmgT120I GGNCC 2 cut(s) 321, 1214
BmiI GGNNCC 4 cut(s) 323, 537, 558, 1215
BmrFI CCNGG 6 cut(s) 276, 319, 380, 914, 1218, 1226
BmrI ACTGGG 1 cut(s) 643
BmsI GCATC 4 cut(s) 217, 688, 778, 942
BmuI ACTGGG 1 cut(s) 643
BpiI GAAGAC 1 cut(s) 1155
BpmI CTGGAG 1 cut(s) 1014
Bpu10I CCTNAGC 1 cut(s) 104
BpuEI CTTGAG 1 cut(s) 1265
BpuMI CCSGG 3 cut(s) 276, 380, 1226
BsaHI GRCGYC 1 cut(s) 157
BsaJI CCNNGG 1 cut(s) 274
BsaWI WCCGGW 2 cut(s) 367, 400
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 3 cut(s) 331, 510, 637
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 3 cut(s) 649, 843, 997
Bse21I CCTNAGG 1 cut(s) 540
BseBI CCWGG 3 cut(s) 319, 914, 1218
BseDI CCNNGG 1 cut(s) 274
BseGI GGATG 4 cut(s) 26, 460, 466, 519
BseLI CCNNNNNNNGG 3 cut(s) 331, 510, 637
BseMII CTCAG 2 cut(s) 531, 798
BseNI ACTGG 3 cut(s) 649, 843, 997
BseRI GAGGAG 9 cut(s) 8, 11, 434, 505, 532, 535, 557, 560, 1103
BseSI GKGCMC 1 cut(s) 1278
BseXI GCAGC 4 cut(s) 157, 254, 793, 1240
Bsh1236I CGCG 3 cut(s) 312, 337, 569
Bsh1285I CGRYCG 1 cut(s) 174
BshFI GGCC 2 cut(s) 5, 503
BsiEI CGRYCG 1 cut(s) 174
BsiHKAI GWGCWC 2 cut(s) 1000, 1278
BsiHKCI CYCGRG 1 cut(s) 608
BsiSI CCGG 6 cut(s) 170, 276, 368, 380, 401, 1226
BslFI GGGAC 2 cut(s) 362, 1077
BslI CCNNNNNNNGG 3 cut(s) 331, 510, 637
BsmAI GTCTC 1 cut(s) 71
BsmBI CGTCTC 1 cut(s) 71
BsmFI GGGAC 2 cut(s) 362, 1077
BsnI GGCC 2 cut(s) 5, 503
BsoBI CYCGRG 1 cut(s) 608
Bsp1286I GDGCHC 2 cut(s) 1000, 1278
Bsp1407I TGTACA 2 cut(s) 682, 1054
Bsp143I GATC 4 cut(s) 98, 220, 495, 1258
BspANI GGCC 2 cut(s) 5, 503
BspCNI CTCAG 2 cut(s) 532, 797
BspFNI CGCG 3 cut(s) 312, 337, 569
BspLI GGNNCC 4 cut(s) 323, 537, 558, 1215
BspMAI CTGCAG 3 cut(s) 811, 1005, 1258
BspPI GGATC 3 cut(s) 93, 215, 503
BsrBI CCGCTC 3 cut(s) 8, 32, 426
BsrFI RCCGGY 1 cut(s) 169
BsrGI TGTACA 2 cut(s) 682, 1054
BsrI ACTGG 3 cut(s) 649, 843, 997
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 1 cut(s) 274
BssMI GATC 4 cut(s) 98, 220, 495, 1258
BssNI GRCGYC 1 cut(s) 157
Bst2UI CCWGG 3 cut(s) 319, 914, 1218
Bst4CI ACNGT 4 cut(s) 670, 962, 976, 1239
Bst6I CTCTTC 2 cut(s) 324, 482
BstACI GRCGYC 1 cut(s) 157
BstAPI GCANNNNNTGC 1 cut(s) 923
BstAUI TGTACA 2 cut(s) 682, 1054
BstC8I GCNNGC 3 cut(s) 34, 856, 1137
BstDEI CTNAG 5 cut(s) 86, 104, 540, 784, 935
BstF5I GGATG 4 cut(s) 26, 460, 466, 519
BstFNI CGCG 3 cut(s) 312, 337, 569
BstHHI GCGC 2 cut(s) 337, 912
BstKTI GATC 4 cut(s) 101, 223, 498, 1261
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 4 cut(s) 98, 220, 495, 1258
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 8 cut(s) 42, 432, 773, 797, 806, 812, 923, 1141
BstNI CCWGG 3 cut(s) 319, 914, 1218
BstNSI RCATGY 1 cut(s) 1061
BstSCI CCNGG 6 cut(s) 274, 317, 378, 912, 1216, 1224
BstSFI CTRYAG 4 cut(s) 807, 1001, 1146, 1254
BstSLI GKGCMC 1 cut(s) 1278
BstUI CGCG 3 cut(s) 312, 337, 569
BstV1I GCAGC 4 cut(s) 157, 254, 793, 1240
BstV2I GAAGAC 1 cut(s) 1155
BstX2I RGATCY 1 cut(s) 98
BstYI RGATCY 1 cut(s) 98
Bsu36I CCTNAGG 1 cut(s) 540
BsuRI GGCC 2 cut(s) 5, 503
BtsCI GGATG 4 cut(s) 26, 460, 466, 519
BtsI GCAGTG 2 cut(s) 40, 998
BtsIMutI CAGTG 3 cut(s) 40, 656, 998
Cac8I GCNNGC 3 cut(s) 34, 856, 1137
CfoI GCGC 2 cut(s) 337, 912
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 2 cut(s) 321, 1214
CseI GACGC 3 cut(s) 165, 301, 629
Csp6I GTAC 4 cut(s) 376, 683, 972, 1055
CviAII CATG 5 cut(s) 616, 680, 727, 740, 1058
CviQI GTAC 4 cut(s) 376, 683, 972, 1055
DdeI CTNAG 5 cut(s) 86, 104, 540, 784, 935
DpnI GATC 4 cut(s) 100, 222, 497, 1260
DpnII GATC 4 cut(s) 98, 220, 495, 1258
DraI TTTAAA 1 cut(s) 1155
DrdI GACNNNNNNGTC 1 cut(s) 311
DseDI GACNNNNNNGTC 1 cut(s) 311
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 324, 482
EarI CTCTTC 2 cut(s) 324, 482
EciI GGCGGA 2 cut(s) 246, 456
Eco47I GGWCC 2 cut(s) 321, 1214
Eco57I CTGAAG 2 cut(s) 32, 798
Eco81I CCTNAGG 1 cut(s) 540
Eco88I CYCGRG 1 cut(s) 608
EcoRI GAATTC 1 cut(s) 930
EcoRII CCWGG 3 cut(s) 317, 912, 1216
Esp3I CGTCTC 1 cut(s) 71
FaeI CATG 5 cut(s) 619, 683, 730, 743, 1061
FalI AAGNNNNNCTT 2 cut(s) 1064, 1096
FaqI GGGAC 2 cut(s) 362, 1077
FatI CATG 5 cut(s) 615, 679, 726, 739, 1057
FauI CCCGC 2 cut(s) 574, 1168
Fnu4HI GCNGC 7 cut(s) 6, 146, 243, 260, 737, 807, 1254
FokI GGATG 4 cut(s) 13, 467, 473, 526
Fsp4HI GCNGC 7 cut(s) 6, 146, 243, 260, 737, 807, 1254
FspBI CTAG 3 cut(s) 153, 801, 968
GlaI GCGC 2 cut(s) 336, 911
GluI GCNGC 7 cut(s) 6, 146, 243, 260, 737, 807, 1254
GsuI CTGGAG 1 cut(s) 1014
HaeIII GGCC 2 cut(s) 5, 503
HapII CCGG 6 cut(s) 170, 276, 368, 380, 401, 1226
HgaI GACGC 3 cut(s) 165, 301, 629
HhaI GCGC 2 cut(s) 337, 912
Hin1I GRCGYC 1 cut(s) 157
Hin1II CATG 5 cut(s) 619, 683, 730, 743, 1061
Hin6I GCGC 2 cut(s) 335, 910
HinP1I GCGC 2 cut(s) 335, 910
HincII GTYRAC 1 cut(s) 877
HindII GTYRAC 1 cut(s) 877
HinfI GANTC 6 cut(s) 113, 130, 232, 281, 298, 1221
HpaII CCGG 6 cut(s) 170, 276, 368, 380, 401, 1226
HphI GGTGA 5 cut(s) 290, 307, 394, 970, 1087
Hpy166II GTNNAC 3 cut(s) 877, 965, 1276
Hpy188I TCNGA 6 cut(s) 12, 112, 118, 135, 513, 787
Hpy188III TCNNGA 4 cut(s) 224, 302, 539, 608
Hpy8I GTNNAC 3 cut(s) 877, 965, 1276
HpyAV CCTTC 6 cut(s) 431, 463, 583, 745, 773, 1000
HpyCH4III ACNGT 4 cut(s) 670, 962, 976, 1239
HpyCH4IV ACGT 1 cut(s) 351
HpyF10VI GCNNNNNNNGC 8 cut(s) 42, 432, 773, 797, 806, 812, 923, 1141
HpyF3I CTNAG 5 cut(s) 86, 104, 540, 784, 935
HpySE526I ACGT 1 cut(s) 351
Hsp92I GRCGYC 1 cut(s) 157
Hsp92II CATG 5 cut(s) 619, 683, 730, 743, 1061
HspAI GCGC 2 cut(s) 335, 910
Kzo9I GATC 4 cut(s) 98, 220, 495, 1258
LmnI GCTCC 6 cut(s) 21, 113, 397, 443, 812, 995
Lsp1109I GCAGC 4 cut(s) 157, 254, 793, 1240
LweI GCATC 4 cut(s) 217, 688, 778, 942
MaeI CTAG 3 cut(s) 153, 801, 968
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 5 cut(s) 38, 278, 313, 722, 976
MalI GATC 4 cut(s) 100, 222, 497, 1260
MbiI CCGCTC 3 cut(s) 8, 32, 426
MboI GATC 4 cut(s) 98, 220, 495, 1258
MboII GAAGA 6 cut(s) 341, 416, 499, 598, 806, 1155
MfeI CAATTG 2 cut(s) 686, 1050
MflI RGATCY 1 cut(s) 98
MhlI GDGCHC 2 cut(s) 1000, 1278
MluCI AATT 6 cut(s) 624, 656, 686, 849, 930, 1050
MlyI GAGTC 3 cut(s) 226, 275, 307
MmeI TCCRAC 2 cut(s) 141, 491
MseI TTAA 4 cut(s) 576, 1154, 1185, 1288
MslI CAYNNNNRTG 1 cut(s) 744
MspI CCGG 6 cut(s) 170, 276, 368, 380, 401, 1226
MspR9I CCNGG 6 cut(s) 276, 319, 380, 914, 1218, 1226
MunI CAATTG 2 cut(s) 686, 1050
MvaI CCWGG 3 cut(s) 319, 914, 1218
MvnI CGCG 3 cut(s) 312, 337, 569
MwoI GCNNNNNNNGC 8 cut(s) 42, 432, 773, 797, 806, 812, 923, 1141
NciI CCSGG 3 cut(s) 276, 380, 1226
NdeII GATC 4 cut(s) 98, 220, 495, 1258
NlaIII CATG 5 cut(s) 619, 683, 730, 743, 1061
NlaIV GGNNCC 4 cut(s) 323, 537, 558, 1215
NmuCI GTSAC 4 cut(s) 38, 278, 313, 976
NspI RCATGY 1 cut(s) 1061
PaeR7I CTCGAG 1 cut(s) 608
PciI ACATGT 1 cut(s) 1057
PfeI GAWTC 3 cut(s) 113, 130, 1221
PflFI GACNNNGTC 1 cut(s) 1017
PfoI TCCNGGA 1 cut(s) 1216
PkrI GCNGC 7 cut(s) 7, 147, 244, 261, 738, 808, 1255
PleI GAGTC 3 cut(s) 226, 275, 306
PpsI GAGTC 3 cut(s) 226, 275, 306
PscI ACATGT 1 cut(s) 1057
Psp6I CCWGG 3 cut(s) 317, 912, 1216
PspGI CCWGG 3 cut(s) 317, 912, 1216
PspN4I GGNNCC 4 cut(s) 323, 537, 558, 1215
PspPI GGNCC 2 cut(s) 321, 1214
PstI CTGCAG 3 cut(s) 811, 1005, 1258
PsuI RGATCY 1 cut(s) 98
PsyI GACNNNGTC 1 cut(s) 1017
RsaI GTAC 4 cut(s) 377, 684, 973, 1056
RsaNI GTAC 4 cut(s) 376, 683, 972, 1055
RseI CAYNNNNRTG 1 cut(s) 744
SaqAI TTAA 4 cut(s) 576, 1154, 1185, 1288
SatI GCNGC 7 cut(s) 6, 146, 243, 260, 737, 807, 1254
Sau3AI GATC 4 cut(s) 98, 220, 495, 1258
Sau96I GGNCC 2 cut(s) 321, 1214
SchI GAGTC 3 cut(s) 226, 275, 307
ScrFI CCNGG 6 cut(s) 276, 319, 380, 914, 1218, 1226
SduI GDGCHC 2 cut(s) 1000, 1278
SfaNI GCATC 4 cut(s) 217, 688, 778, 942
SfcI CTRYAG 4 cut(s) 807, 1001, 1146, 1254
Sfr274I CTCGAG 1 cut(s) 608
SinI GGWCC 2 cut(s) 321, 1214
SlaI CTCGAG 1 cut(s) 608
SmiMI CAYNNNNRTG 1 cut(s) 744
SmlI CTYRAG 2 cut(s) 608, 1280
SmoI CTYRAG 2 cut(s) 608, 1280
Sse9I AATT 6 cut(s) 624, 656, 686, 849, 930, 1050
SspMI CTAG 3 cut(s) 153, 801, 968
StyD4I CCNGG 6 cut(s) 274, 317, 378, 912, 1216, 1224
TaaI ACNGT 4 cut(s) 670, 962, 976, 1239
TaiI ACGT 1 cut(s) 354
TaqI TCGA 4 cut(s) 174, 296, 498, 609
TasI AATT 6 cut(s) 624, 656, 686, 849, 930, 1050
TatI WGTACW 2 cut(s) 682, 1054
TauI GCSGC 3 cut(s) 8, 262, 739
TfiI GAWTC 3 cut(s) 113, 130, 1221
Tru1I TTAA 4 cut(s) 576, 1154, 1185, 1288
Tru9I TTAA 4 cut(s) 576, 1154, 1185, 1288
TscAI CASTG 3 cut(s) 47, 656, 1005
TseFI GTSAC 4 cut(s) 38, 278, 313, 976
TseI GCWGC 4 cut(s) 145, 242, 806, 1253
Tsp45I GTSAC 4 cut(s) 38, 278, 313, 976
TspDTI ATGAA 3 cut(s) 331, 678, 912
TspGWI ACGGA 1 cut(s) 1100
TspRI CASTG 3 cut(s) 47, 656, 1005
Tth111I GACNNNGTC 1 cut(s) 1017
VneI GTGCAC 1 cut(s) 1274
VpaK11BI GGWCC 2 cut(s) 321, 1214
XapI RAATTY 2 cut(s) 656, 930
XceI RCATGY 1 cut(s) 1061
XhoI CTCGAG 1 cut(s) 608
XspI CTAG 3 cut(s) 153, 801, 968
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.