pycom09g17760

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0007066
Physical Location & Seq
Reverse (-)
18248778 .. 18251639
2862 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2586 bp
ATGGCAACCAACAAGTTTGCAAGCATGTTGCACAGGAACACTCCCATGTTGATTGTAATTCTAGTCTATGCTGTTCTTGAATGGATTTTGATAATACTCCTCCTCCTCGACTCTTTGCTCAGTTACTTGATCAACAAATATGCCAACTACTTCGGCCTGAAACCATCTTGTCCGTTGTGTACTCCTAGAATCAAACGCGTCCAGGAACAAGGCAAGAGCTCAAACAACTCTTACACAGATGTTGTATGTGAGAGCCATGCCACTCAGATATCCAACATGTTTCGCCGTCCTTGTTCCGAACCTTCTTGCGGTTCTTTGGAGTATGTGCGGAAAGGGAATTCAGCTGTAGAAGCTCTGGATGATGGAAGCAATGGAGGTAAGTATCAAAGCGCAAACAAACCTGAAAGCCTGTCAACTCACAGTTACAATGGCTATGAGTTGGAGACAACGAATGAAGAACATGAGGACGATAGAGTGGCAGACGAGCAAAAACTTACATCGGATGTTTGCAGTTCTAGCTTCAGAGGCATTGCAGTGGAAGATTGTCAAAGGTCTAGATCAGTATTGCTATGTCAGGAAAATGATGCAGATGAAGATAACAAAGGAGGTTCTCTTGACATTGCGCGGTCAGATTCTAACAGCACGAAACTTGTCCATCAATCTTCCGATGCTAGTGCTACTAGCATTCAGTGTTGCTTTGAAGAAGATTACTCTCTTGAAATCATCCCCCTATCTTCCGAGAATGGTTTGATCTGTGCTCACAATCATCGGTTGATTCCCGTTGAGTTGATTGATTTTTCAACTACAGTACACCAAGTAACCCGCAATGTAAAAGAGGAGGTGAGAGAGCATGATCATAAGGAATTAAATTTCGCCTCTGAGTCGAACATCGGATCTGAACCTCAACTCTCTGGAGAACCATCATTAGTACTTGTCACAAACGAGAGTGCAGTTAACAAAAGTAATGGAGAAGTCGAAGGCTTAGATATGTCCAGGGGTTTTACTGATAACTCTTCCGACGTAGACGCGGAAGAGGTAAAACAAGATTTGGTTTGCAAGCCATGTGACGAAGTTGTCACTGCTCCAGGAGCTCAAACATTATTTGTTCGAATTGAAGAACCCGATAAGAAAGAACGAAATGATCCACCAGCATCTGAAGAAGAAAGTATTTTTGCGAACACAGAAGAGTATGACCCTGCAAGTGATCACCCTCGGGCTCAAGAAGGTACAAGGCATACTGAAGCAGCTACACCGCAAGAGGAAACAAGTATGCCTGAAAAGGCTCAAGAACGAGGGGAAGAGAAGATTCCTGACTCACCAACATCTGTAAACAGTCTTTGTTCCTTGCACAAGAAACCAGATGATAAAATAGAGTCGGCGGTAGAAGAGTATTTGGATGGAAGTGCGGTGAGCGAGACGGAAGAGAGGGATCCTTTTGCAACCATTGGACGGCTCAAAGAAGCGCTGACAGCTGAGCGTAAATCTCTAAGTTCTTTATATGCAGAACTAGAGGAAGAGAGGAGTGCGTCTGCAATTGCCGCCAACCAGACTATGGCTATGATAACAAGGCTTCAAGAAGAGAAGGCAGCAATGCAAATGGAGGCATTGCAATACCAGAGGATGATGGAAGAACAATCCGAGTACGACCAGGATGCCTTACAACTTCTGAATGATCTGATGAACAAGAAGGAGAAGGAAAAGCAAGAACTAGAGAGAGAATTGGAAATATATCAAAAGAAAGTCCAAGATTATGAGGAAAAAGAGAAGGCAAGGACCCTGAGCAGAATTAAATATGGAAGTATAAGAAGTAGGAATTCTTCTGCTTCTTGCAGCCAGTCGTGGGATAGTGATGCGCTATCGATTGATCTTAATGTTGAAGTTAGGGACGAAGAAAGCGGCTTCGGTGGCCATCAAGAAAGCAATGACAAAAACAATACCACAGACGATGCAGGTCTGAGTTTGGAAGATTTGGCTCTGGATTGTGTTAAAAATATGAGTGTTCTTGATGAATCACTAGCAGGATTCGAGGAAGAGAGGCTGTCCATTCTGGATCAGCTCAAAGCCTTGGAGGAGAAGCTAATCACATTGGGTGCAAATGAGGAATTAGCCGAGGACTTTAAGCTAGTTGAGCATTCCCCAACGTACAGCCTCACATACTCCGAAGAGAATCATGATGTTAGCAGTCCAGAAGAAAATGGGATTGATAGACATCATCCCGAGGAAAAGACATTGGATTCCATGGCAAAGAGGCTTCTTCCGCTTTTGGATGCAACTGACGATGAAACCGAAGAAGGGTTAACTCATGAAGAACACGCTCATCAATCAGAATCAATTGAAATGATGCGGAGCTCAATTACCAATTTCGAACTGAATGATGGAAAAATAGCCATTGAAGAGGAGGTGGATCATGTTTATGAGAGGCTACAAGCTCTTGAAGCAGACAGGGAGTTTCTCAAGCACTGCATGGGCTCCATACAGAAGGGAGACAAAGGGGTGGATCTTCTCCAAGAGATTTTGCAACATCTGCGGGATCTGAGGGTTGTTGAACTCCGCGTCAAGAACATGAATCGATCCCGAGGGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

862

Amino Acids

96.7

Weight (kDa)

4.6

Isoelectric Point (pI)

55.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 484 - 574 6.8e-33 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1073
Acc36I ACCTGC 1 cut(s) 1940
AccB7I CCANNNNNTGG 1 cut(s) 1552
AccI GTMKAC 1 cut(s) 1023
AccII CGCG 4 cut(s) 198, 625, 1028, 2553
AclWI GGATC 9 cut(s) 901, 1136, 1424, 1437, 2058, 2412, 2505, 2538, 2565
AcoI YGGCCR 1 cut(s) 1906
AcsI RAATTY 3 cut(s) 337, 868, 1813
AcuI CTGAAG 3 cut(s) 505, 1176, 1260
AfaI GTAC 6 cut(s) 181, 810, 930, 1228, 1643, 2144
AfeI AGCGCT 1 cut(s) 1464
AfiI CCNNNNNNNGG 5 cut(s) 308, 1449, 1552, 1840, 2292
AflIII ACRYGT 2 cut(s) 196, 276
AjnI CCWGG 4 cut(s) 201, 992, 1084, 1647
Alw21I GWGCWC 4 cut(s) 221, 760, 1093, 2351
Alw26I GTCTC 3 cut(s) 437, 1409, 2478
AlwI GGATC 9 cut(s) 901, 1136, 1424, 1437, 2058, 2412, 2505, 2538, 2565
AlwNI CAGNNNCTG 1 cut(s) 1154
Ama87I CYCGRG 3 cut(s) 1212, 2216, 2574
Aor51HI AGCGCT 1 cut(s) 1464
AoxI GGCC 2 cut(s) 154, 1906
ApeKI GCWGC 3 cut(s) 1244, 1586, 1830
ApoI RAATTY 3 cut(s) 337, 868, 1813
AspLEI GCGC 4 cut(s) 392, 625, 1465, 1855
AspS9I GGNCC 1 cut(s) 1773
AsuHPI GGTGA 4 cut(s) 853, 1199, 1308, 1420
AsuII TTCGAA 2 cut(s) 1108, 2364
AvaI CYCGRG 3 cut(s) 1212, 2216, 2574
AvaII GGWCC 1 cut(s) 1773
BalI TGGCCA 1 cut(s) 1908
BamHI GGATCC 1 cut(s) 1429
BanII GRGCYC 5 cut(s) 221, 1093, 1219, 2351, 2471
BarI GAAGNNNNNNTAC 2 cut(s) 1801, 1833
Bbv12I GWGCWC 4 cut(s) 221, 760, 1093, 2351
BbvI GCAGC 3 cut(s) 1256, 1598, 1842
BccI CCATC 8 cut(s) 172, 356, 663, 928, 1391, 1618, 1917, 2369
BceAI ACGGC 2 cut(s) 270, 1466
BcgI CGANNNNNNTGC 4 cut(s) 656, 690, 1634, 1668
BciT130I CCWGG 4 cut(s) 203, 994, 1086, 1649
BclI TGATCA 3 cut(s) 129, 853, 1204
BcoDI GTCTC 3 cut(s) 437, 1409, 2478
BfmI CTRYAG 2 cut(s) 345, 804
BfoI RGCGCY 1 cut(s) 1466
BfuAI ACCTGC 1 cut(s) 1940
BisI GCNGC 5 cut(s) 1245, 1539, 1587, 1831, 1897
BlpI GCTNAGC 1 cut(s) 1473
BlsI GCNGC 5 cut(s) 1246, 1540, 1588, 1832, 1898
BmcAI AGTACT 1 cut(s) 930
Bme1390I CCNGG 4 cut(s) 203, 994, 1086, 1649
Bme18I GGWCC 1 cut(s) 1773
BmeT110I CYCGRG 3 cut(s) 1212, 2216, 2574
BmgT120I GGNCC 1 cut(s) 1773
BmiI GGNNCC 3 cut(s) 1431, 1775, 2470
BmrFI CCNGG 4 cut(s) 203, 994, 1086, 1649
BmsI GCATC 8 cut(s) 574, 658, 1160, 1642, 1840, 1936, 2257, 2331
BpmI CTGGAG 2 cut(s) 933, 1068
Bpu10I CCTNAGC 1 cut(s) 1778
Bpu1102I GCTNAGC 1 cut(s) 1473
Bpu14I TTCGAA 2 cut(s) 1108, 2364
BpuEI CTTGAG 3 cut(s) 1203, 1269, 2438
Bsa29I ATCGAT 2 cut(s) 1859, 2569
BsaBI GATNNNNATC 1 cut(s) 2208
BsaJI CCNNGG 7 cut(s) 993, 1211, 2064, 2109, 2217, 2238, 2575
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bsc4I CCNNNNNNNGG 5 cut(s) 308, 1449, 1552, 1840, 2292
Bse1I ACTGG 1 cut(s) 1834
Bse3DI GCAATG 7 cut(s) 376, 528, 618, 832, 1596, 1604, 1927
Bse8I GATNNNNATC 1 cut(s) 2208
BseBI CCWGG 4 cut(s) 203, 994, 1086, 1649
BseCI ATCGAT 2 cut(s) 1859, 2569
BseDI CCNNGG 7 cut(s) 993, 1211, 2064, 2109, 2217, 2238, 2575
BseGI GGATG 8 cut(s) 364, 508, 723, 1402, 1626, 1657, 2212, 2272
BseJI GATNNNNATC 1 cut(s) 2208
BseLI CCNNNNNNNGG 5 cut(s) 308, 1449, 1552, 1840, 2292
BseMI GCAATG 7 cut(s) 376, 528, 618, 832, 1596, 1604, 1927
BseMII CTCAG 7 cut(s) 133, 278, 870, 1464, 1769, 1946, 2525
BseNI ACTGG 1 cut(s) 1834
BseRI GAGGAG 7 cut(s) 89, 92, 95, 851, 1534, 2084, 2411
BseXI GCAGC 3 cut(s) 1256, 1598, 1842
BsgI GTGCAG 1 cut(s) 969
Bsh1236I CGCG 4 cut(s) 198, 625, 1028, 2553
BshFI GGCC 2 cut(s) 156, 1908
BshVI ATCGAT 2 cut(s) 1859, 2569
BsiHKAI GWGCWC 4 cut(s) 221, 760, 1093, 2351
BsiHKCI CYCGRG 3 cut(s) 1212, 2216, 2574
BslFI GGGAC 1 cut(s) 1898
BslI CCNNNNNNNGG 5 cut(s) 308, 1449, 1552, 1840, 2292
BsmAI GTCTC 3 cut(s) 437, 1409, 2478
BsmBI CGTCTC 1 cut(s) 1409
BsmFI GGGAC 1 cut(s) 1898
BsmI GAATGC 2 cut(s) 684, 2131
BsnI GGCC 2 cut(s) 156, 1908
BsoBI CYCGRG 3 cut(s) 1212, 2216, 2574
Bsp119I TTCGAA 2 cut(s) 1108, 2364
Bsp1286I GDGCHC 6 cut(s) 221, 760, 1093, 1219, 2351, 2471
Bsp1720I GCTNAGC 1 cut(s) 1473
Bsp19I CCATGG 1 cut(s) 2238
BspANI GGCC 2 cut(s) 156, 1908
BspCNI CTCAG 7 cut(s) 132, 277, 871, 1465, 1770, 1947, 2526
BspDI ATCGAT 2 cut(s) 1859, 2569
BspFNI CGCG 4 cut(s) 198, 625, 1028, 2553
BspHI TCATGA 2 cut(s) 2170, 2302
BspLI GGNNCC 3 cut(s) 1431, 1775, 2470
BspMI ACCTGC 1 cut(s) 1940
BspPI GGATC 9 cut(s) 901, 1136, 1424, 1437, 2058, 2412, 2505, 2538, 2565
BspT104I TTCGAA 2 cut(s) 1108, 2364
BsrDI GCAATG 7 cut(s) 376, 528, 618, 832, 1596, 1604, 1927
BsrI ACTGG 1 cut(s) 1834
BssECI CCNNGG 7 cut(s) 993, 1211, 2064, 2109, 2217, 2238, 2575
BssT1I CCWWGG 2 cut(s) 2064, 2238
Bst2UI CCWGG 4 cut(s) 203, 994, 1086, 1649
Bst4CI ACNGT 3 cut(s) 422, 808, 1334
BstAPI GCANNNNNTGC 1 cut(s) 2524
BstBI TTCGAA 2 cut(s) 1108, 2364
BstC8I GCNNGC 2 cut(s) 22, 1058
BstDEI CTNAG 9 cut(s) 119, 264, 879, 982, 1473, 1487, 1778, 1955, 2534
BstDSI CCRYGG 1 cut(s) 2238
BstF5I GGATG 8 cut(s) 364, 508, 723, 1402, 1626, 1657, 2212, 2272
BstFNI CGCG 4 cut(s) 198, 625, 1028, 2553
BstH2I RGCGCY 1 cut(s) 1466
BstHHI GCGC 4 cut(s) 392, 625, 1465, 1855
BstMAI GTCTC 3 cut(s) 437, 1409, 2478
BstNI CCWGG 4 cut(s) 203, 994, 1086, 1649
BstNSI RCATGY 2 cut(s) 28, 280
BstSCI CCNGG 4 cut(s) 201, 992, 1084, 1647
BstSFI CTRYAG 2 cut(s) 345, 804
BstUI CGCG 4 cut(s) 198, 625, 1028, 2553
BstV1I GCAGC 3 cut(s) 1256, 1598, 1842
BstX2I RGATCY 4 cut(s) 893, 1429, 2497, 2530
BstYI RGATCY 4 cut(s) 893, 1429, 2497, 2530
Bsu15I ATCGAT 2 cut(s) 1859, 2569
BsuRI GGCC 2 cut(s) 156, 1908
BsuTUI ATCGAT 2 cut(s) 1859, 2569
BtgI CCRYGG 1 cut(s) 2238
BtsCI GGATG 8 cut(s) 364, 508, 723, 1402, 1626, 1657, 2212, 2272
BtsI GCAGTG 3 cut(s) 540, 1077, 2458
BtsIMutI CAGTG 4 cut(s) 540, 695, 1077, 2458
BveI ACCTGC 1 cut(s) 1940
Cac8I GCNNGC 2 cut(s) 22, 1058
CaiI CAGNNNCTG 1 cut(s) 1154
CciI TCATGA 2 cut(s) 2170, 2302
CfoI GCGC 4 cut(s) 392, 625, 1465, 1855
Cfr13I GGNCC 1 cut(s) 1773
ClaI ATCGAT 2 cut(s) 1859, 2569
CseI GACGC 4 cut(s) 187, 1034, 1515, 2542
Csp6I GTAC 6 cut(s) 180, 809, 929, 1227, 1642, 2143
CviQI GTAC 6 cut(s) 180, 809, 929, 1227, 1642, 2143
DdeI CTNAG 9 cut(s) 119, 264, 879, 982, 1473, 1487, 1778, 1955, 2534
DrdI GACNNNNNNGTC 1 cut(s) 1073
DseDI GACNNNNNNGTC 1 cut(s) 1073
EaeI YGGCCR 1 cut(s) 1906
Ecl136II GAGCTC 3 cut(s) 219, 1091, 2349
Eco130I CCWWGG 2 cut(s) 2064, 2238
Eco24I GRGCYC 5 cut(s) 221, 1093, 1219, 2351, 2471
Eco32I GATATC 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 1773
Eco47III AGCGCT 1 cut(s) 1464
Eco53kI GAGCTC 3 cut(s) 219, 1091, 2349
Eco57I CTGAAG 3 cut(s) 505, 1176, 1260
Eco88I CYCGRG 3 cut(s) 1212, 2216, 2574
EcoICRI GAGCTC 3 cut(s) 219, 1091, 2349
EcoO109I RGGNCCY 1 cut(s) 1773
EcoRI GAATTC 2 cut(s) 337, 1813
EcoRII CCWGG 4 cut(s) 201, 992, 1084, 1647
EcoRV GATATC 1 cut(s) 270
EcoT14I CCWWGG 2 cut(s) 2064, 2238
EcoT38I GRGCYC 5 cut(s) 221, 1093, 1219, 2351, 2471
ErhI CCWWGG 2 cut(s) 2064, 2238
Esp3I CGTCTC 1 cut(s) 1409
FaqI GGGAC 1 cut(s) 1898
FauI CCCGC 2 cut(s) 830, 2520
FbaI TGATCA 3 cut(s) 129, 853, 1204
FblI GTMKAC 1 cut(s) 1023
Fnu4HI GCNGC 5 cut(s) 1245, 1539, 1587, 1831, 1897
FokI GGATG 8 cut(s) 371, 515, 710, 1409, 1633, 1664, 2199, 2279
FriOI GRGCYC 5 cut(s) 221, 1093, 1219, 2351, 2471
Fsp4HI GCNGC 5 cut(s) 1245, 1539, 1587, 1831, 1897
GlaI GCGC 4 cut(s) 391, 624, 1464, 1854
GluI GCNGC 5 cut(s) 1245, 1539, 1587, 1831, 1897
GsuI CTGGAG 2 cut(s) 933, 1068
HaeII RGCGCY 1 cut(s) 1466
HaeIII GGCC 2 cut(s) 156, 1908
HgaI GACGC 4 cut(s) 187, 1034, 1515, 2542
HhaI GCGC 4 cut(s) 392, 625, 1465, 1855
Hin6I GCGC 4 cut(s) 390, 623, 1463, 1853
HinP1I GCGC 4 cut(s) 390, 623, 1463, 1853
HincII GTYRAC 3 cut(s) 414, 955, 2298
HindII GTYRAC 3 cut(s) 414, 955, 2298
HpaI GTTAAC 2 cut(s) 955, 2298
HphI GGTGA 4 cut(s) 853, 1199, 1308, 1420
Hpy166II GTNNAC 7 cut(s) 180, 414, 811, 955, 1024, 1330, 2298
Hpy8I GTNNAC 7 cut(s) 180, 414, 811, 955, 1024, 1330, 2298
Hpy99I CGWCG 1 cut(s) 1022
HpyAV CCTTC 9 cut(s) 312, 971, 1217, 1576, 1681, 1687, 1759, 2285, 2473
HpyCH4III ACNGT 3 cut(s) 422, 808, 1334
HpyCH4IV ACGT 2 cut(s) 1020, 2141
HpyF3I CTNAG 9 cut(s) 119, 264, 879, 982, 1473, 1487, 1778, 1955, 2534
HpySE526I ACGT 2 cut(s) 1020, 2141
HspAI GCGC 4 cut(s) 390, 623, 1463, 1853
Ksp22I TGATCA 3 cut(s) 129, 853, 1204
KspAI GTTAAC 2 cut(s) 955, 2298
LmnI GCTCC 4 cut(s) 1087, 1088, 2346, 2474
Lsp1109I GCAGC 3 cut(s) 1256, 1598, 1842
LweI GCATC 8 cut(s) 574, 658, 1160, 1642, 1840, 1936, 2257, 2331
MaeII ACGT 2 cut(s) 1020, 2141
MaeIII GTNAC 6 cut(s) 122, 422, 817, 934, 1064, 1075
MfeI CAATTG 2 cut(s) 1533, 2331
MflI RGATCY 4 cut(s) 893, 1429, 2497, 2530
MhlI GDGCHC 6 cut(s) 221, 760, 1093, 1219, 2351, 2471
MlsI TGGCCA 1 cut(s) 1908
MluI ACGCGT 1 cut(s) 196
MluNI TGGCCA 1 cut(s) 1908
MlyI GAGTC 4 cut(s) 104, 890, 1307, 1382
MmeI TCCRAC 3 cut(s) 297, 420, 1041
Mox20I TGGCCA 1 cut(s) 1908
MscI TGGCCA 1 cut(s) 1908
MseI TTAA 7 cut(s) 866, 954, 1788, 1869, 1986, 2118, 2297
MslI CAYNNNNRTG 3 cut(s) 44, 533, 2412
Msp20I TGGCCA 1 cut(s) 1908
MspA1I CMGCKG 2 cut(s) 344, 1472
MspR9I CCNGG 4 cut(s) 203, 994, 1086, 1649
MunI CAATTG 2 cut(s) 1533, 2331
Mva1269I GAATGC 2 cut(s) 684, 2131
MvaI CCWGG 4 cut(s) 203, 994, 1086, 1649
MvnI CGCG 4 cut(s) 198, 625, 1028, 2553
NcoI CCATGG 1 cut(s) 2238
NlaIV GGNNCC 3 cut(s) 1431, 1775, 2470
NmeAIII GCCGAG 1 cut(s) 2134
NmuCI GTSAC 3 cut(s) 934, 1064, 1075
NspI RCATGY 2 cut(s) 28, 280
NspV TTCGAA 2 cut(s) 1108, 2364
PagI TCATGA 2 cut(s) 2170, 2302
PciI ACATGT 1 cut(s) 276
PcsI WCGNNNNNNNCGW 1 cut(s) 2283
PctI GAATGC 2 cut(s) 684, 2131
PflMI CCANNNNNTGG 1 cut(s) 1552
PfoI TCCNGGA 2 cut(s) 201, 1084
PkrI GCNGC 5 cut(s) 1246, 1540, 1588, 1832, 1898
PleI GAGTC 4 cut(s) 104, 889, 1307, 1381
PpsI GAGTC 4 cut(s) 104, 889, 1307, 1381
PpuMI RGGWCCY 1 cut(s) 1773
PscI ACATGT 1 cut(s) 276
Psp124BI GAGCTC 3 cut(s) 221, 1093, 2351
Psp5II RGGWCCY 1 cut(s) 1773
Psp6I CCWGG 4 cut(s) 201, 992, 1084, 1647
PspGI CCWGG 4 cut(s) 201, 992, 1084, 1647
PspN4I GGNNCC 3 cut(s) 1431, 1775, 2470
PspPI GGNCC 1 cut(s) 1773
PspPPI RGGWCCY 1 cut(s) 1773
PstNI CAGNNNCTG 1 cut(s) 1154
PsuI RGATCY 4 cut(s) 893, 1429, 2497, 2530
PvuII CAGCTG 2 cut(s) 344, 1472
RsaI GTAC 6 cut(s) 181, 810, 930, 1228, 1643, 2144
RsaNI GTAC 6 cut(s) 180, 809, 929, 1227, 1642, 2143
RseI CAYNNNNRTG 3 cut(s) 44, 533, 2412
SacI GAGCTC 3 cut(s) 221, 1093, 2351
SaqAI TTAA 7 cut(s) 866, 954, 1788, 1869, 1986, 2118, 2297
SatI GCNGC 5 cut(s) 1245, 1539, 1587, 1831, 1897
Sau96I GGNCC 1 cut(s) 1773
ScaI AGTACT 1 cut(s) 930
SchI GAGTC 4 cut(s) 104, 890, 1307, 1382
ScrFI CCNGG 4 cut(s) 203, 994, 1086, 1649
SduI GDGCHC 6 cut(s) 221, 760, 1093, 1219, 2351, 2471
SfaNI GCATC 8 cut(s) 574, 658, 1160, 1642, 1840, 1936, 2257, 2331
SfcI CTRYAG 2 cut(s) 345, 804
SfuI TTCGAA 2 cut(s) 1108, 2364
SinI GGWCC 1 cut(s) 1773
SmiMI CAYNNNNRTG 3 cut(s) 44, 533, 2412
SmlI CTYRAG 3 cut(s) 1218, 1284, 2453
SmoI CTYRAG 3 cut(s) 1218, 1284, 2453
SstI GAGCTC 3 cut(s) 221, 1093, 2351
StyD4I CCNGG 4 cut(s) 201, 992, 1084, 1647
StyI CCWWGG 2 cut(s) 2064, 2238
TaaI ACNGT 3 cut(s) 422, 808, 1334
TaiI ACGT 2 cut(s) 1023, 2144
TaqI TCGA 8 cut(s) 108, 884, 975, 1108, 1859, 2025, 2364, 2569
TatI WGTACW 3 cut(s) 179, 808, 928
TauI GCSGC 2 cut(s) 1541, 1899
Tru1I TTAA 7 cut(s) 866, 954, 1788, 1869, 1986, 2118, 2297
Tru9I TTAA 7 cut(s) 866, 954, 1788, 1869, 1986, 2118, 2297
TscAI CASTG 4 cut(s) 540, 695, 1084, 2465
TseFI GTSAC 3 cut(s) 934, 1064, 1075
TseI GCWGC 3 cut(s) 1244, 1586, 1830
Tsp45I GTSAC 3 cut(s) 934, 1064, 1075
TspDTI ATGAA 7 cut(s) 468, 606, 1694, 2022, 2295, 2319, 2579
TspGWI ACGGA 2 cut(s) 162, 1433
TspRI CASTG 4 cut(s) 540, 695, 1084, 2465
Van91I CCANNNNNTGG 1 cut(s) 1552
VpaK11BI GGWCC 1 cut(s) 1773
XapI RAATTY 3 cut(s) 337, 868, 1813
XbaI TCTAGA 1 cut(s) 554
XceI RCATGY 2 cut(s) 28, 280
XcmI CCANNNNNNNNNTGG 1 cut(s) 1549
XmiI GTMKAC 1 cut(s) 1023
ZrmI AGTACT 1 cut(s) 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.