pycom1094g00120

Lys-63-specific deubiquitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00001094
OG0007344
Physical Location & Seq
Forward (+)
227148 .. 230192
3045 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 966 bp
ATGTATCAACTTCTAGATTCTGGGTTTATCGGGCTCATATTTTCCTGCTTTAGTGAAGATATAAACAAGGTTGGGCGAATCCAAGTCATTGCTTTTCAGTCCTCGGATGGGAAACAGCATCACATGTCAAGACCTATATCTTTATCTCCTGTACATAAAAGTCCAGTTATAGATGTTGAATCGTCTTTAAGTACCTCAGAAAATGCACCAGCGAGATCGATCTTAACTAGAGCAGGGAGTCCTGAACAAGACACAGCTGATTCAAGAACTTCTGGAACTATAAAGGGTGGGGGGAGGTCTTCAGACCTGGGGCTTTTCTTTGCCAATGCTGATGCAAGTCATCTAGGAAGAGTTGGAGGAAACTACCCGACTGACACTTCAAGCAACATCATTGGCGATATAGATCCCATGGATATGTCAGAGAGTATGCAAGAAGCAATGCACCGTTCTAACTTGGATATGAGTGGTGCTGAGTATGCCAGGAAAGAAGTTCCTCTTCACGTTTTACCGACCTCATCTCTCGTGAAGCTCAATTCACCTTTAATGTCATTCACCGAATTGCAACGAGTACTGTATGAAGAGGAGCGAGCGGCTTATAACCAAGCAATCTTGCAAAATATGAGGGATGGGAAAGTGCATCCTCTTACTTTCATACATCACACATCAATGTACCAGTCTTCCATGTGCAAATTAATCGAATATTGTTTGAGTCCTGCCATAAGTGCACTGCAATGTCGGTTAAGAGAGAATGAAACTCGGTTAGCAGTGCTCACCGATCAAGTGAAGATTTTGGAAACTGAGACGCATAGAATGGCTGAGACAAATTCAGGATCTCCTCGTCCAGTTTCATCTCCTGTACTCCGTGGGAGTGCTCCGTTTGGTCACAGGGAAATGTTTAGTCCAGCTGAATCTGTCAGTGCAAGGAGTCCAGCTGGTTCTGGCAGTAGGAGCCGAAAGGGTTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0003674 GO:0003824 GO:0004843 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006282 GO:0006302 GO:0006325 GO:0006464 GO:0006508 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0008237 GO:0009314 GO:0009628 GO:0009893 GO:0009987 GO:0010212 GO:0010564 GO:0010604 GO:0010948 GO:0016043 GO:0016569 GO:0016570 GO:0016578 GO:0016579 GO:0016787 GO:0019219 GO:0019222 GO:0019538 GO:0019783 GO:0023052 GO:0031323 GO:0031325 GO:0031570 GO:0031572 GO:0031593 GO:0032991 GO:0033554 GO:0034641 GO:0035556 GO:0036211 GO:0036459 GO:0042770 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045739 GO:0045786 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051276 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0070011 GO:0070531 GO:0070536 GO:0070537 GO:0070552 GO:0070646 GO:0070647 GO:0071704 GO:0071840 GO:0072395 GO:0072401 GO:0072422 GO:0072425 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0101005 GO:0140030 GO:0140096 GO:1901360 GO:1901564 GO:1901987 GO:1901988 GO:1902749 GO:1902750 GO:2001020 GO:2001022
KEGG Pathways
Metabolic & Signaling
ko03440 ko04621 map03440 map04621
Pfam Domains
Protein Families
JAB

Protein Analysis

322

Amino Acids

35.01

Weight (kDa)

6.42

Isoelectric Point (pI)

74.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRCC36_C PF18110 181 - 263 1.3e-22 BRCC36 C-terminal helical domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 597
AccBSI CCGCTC 1 cut(s) 590
AciI CCGC 1 cut(s) 590
AclWI GGATC 2 cut(s) 398, 838
AcsI RAATTY 1 cut(s) 823
AcuI CTGAAG 1 cut(s) 285
AfaI GTAC 5 cut(s) 153, 193, 570, 671, 858
AfiI CCNNNNNNNGG 1 cut(s) 108
AflIII ACRYGT 1 cut(s) 123
AgsI TTSAA 3 cut(s) 179, 264, 381
AjnI CCWGG 2 cut(s) 306, 479
AluBI AGCT 4 cut(s) 257, 529, 905, 932
AluI AGCT 4 cut(s) 257, 529, 905, 932
Alw21I GWGCWC 3 cut(s) 727, 771, 874
Alw26I GTCTC 2 cut(s) 794, 812
Alw44I GTGCAC 1 cut(s) 723
AlwI GGATC 2 cut(s) 398, 838
ApaLI GTGCAC 1 cut(s) 723
ApoI RAATTY 1 cut(s) 823
AseI ATTAAT 1 cut(s) 692
Asp700I GAANNNNTTC 1 cut(s) 958
AsuHPI GGTGA 3 cut(s) 528, 544, 763
BaeGI GKGCMC 1 cut(s) 727
BanII GRGCYC 1 cut(s) 36
BauI CACGAG 1 cut(s) 521
BbsI GAAGAC 2 cut(s) 291, 669
Bbv12I GWGCWC 3 cut(s) 727, 771, 874
BccI CCATC 2 cut(s) 101, 620
BcgI CGANNNNNNTGC 2 cut(s) 676, 710
BciT130I CCWGG 2 cut(s) 308, 481
BcoDI GTCTC 2 cut(s) 794, 812
BfaI CTAG 3 cut(s) 14, 228, 344
BisI GCNGC 1 cut(s) 591
BlsI GCNGC 1 cut(s) 592
BmcAI AGTACT 1 cut(s) 570
Bme1390I CCNGG 2 cut(s) 308, 481
BmiI GGNNCC 1 cut(s) 950
BmrFI CCNGG 2 cut(s) 308, 481
BmsI GCATC 3 cut(s) 127, 322, 646
BpiI GAAGAC 2 cut(s) 291, 669
Bsa29I ATCGAT 1 cut(s) 218
BsaBI GATNNNNATC 1 cut(s) 402
BsaJI CCNNGG 4 cut(s) 102, 307, 408, 862
Bsc4I CCNNNNNNNGG 1 cut(s) 108
Bse1I ACTGG 3 cut(s) 164, 673, 842
Bse3DI GCAATG 3 cut(s) 87, 444, 737
Bse8I GATNNNNATC 1 cut(s) 402
BseBI CCWGG 2 cut(s) 308, 481
BseCI ATCGAT 1 cut(s) 218
BseDI CCNNGG 4 cut(s) 102, 307, 408, 862
BseGI GGATG 3 cut(s) 112, 631, 637
BseJI GATNNNNATC 1 cut(s) 402
BseLI CCNNNNNNNGG 1 cut(s) 108
BseMI GCAATG 3 cut(s) 87, 444, 737
BseMII CTCAG 4 cut(s) 210, 462, 789, 807
BseNI ACTGG 3 cut(s) 164, 673, 842
BseRI GAGGAG 2 cut(s) 596, 825
BseSI GKGCMC 1 cut(s) 727
BshVI ATCGAT 1 cut(s) 218
BsiHKAI GWGCWC 3 cut(s) 727, 771, 874
BslI CCNNNNNNNGG 1 cut(s) 108
BsmAI GTCTC 2 cut(s) 794, 812
BsmBI CGTCTC 1 cut(s) 794
Bsp1286I GDGCHC 4 cut(s) 36, 727, 771, 874
Bsp1407I TGTACA 1 cut(s) 151
Bsp143I GATC 5 cut(s) 215, 219, 403, 775, 830
Bsp19I CCATGG 1 cut(s) 408
BspACI CCGC 1 cut(s) 590
BspCNI CTCAG 4 cut(s) 209, 463, 790, 808
BspDI ATCGAT 1 cut(s) 218
BspLI GGNNCC 1 cut(s) 950
BspPI GGATC 2 cut(s) 398, 838
BsrBI CCGCTC 1 cut(s) 590
BsrDI GCAATG 3 cut(s) 87, 444, 737
BsrGI TGTACA 1 cut(s) 151
BsrI ACTGG 3 cut(s) 164, 673, 842
BssECI CCNNGG 4 cut(s) 102, 307, 408, 862
BssMI GATC 5 cut(s) 215, 219, 403, 775, 830
BssSI CACGAG 1 cut(s) 521
BssT1I CCWWGG 1 cut(s) 408
Bst2BI CACGAG 1 cut(s) 521
Bst2UI CCWGG 2 cut(s) 308, 481
Bst4CI ACNGT 2 cut(s) 446, 573
Bst6I CTCTTC 3 cut(s) 343, 501, 573
BstAUI TGTACA 1 cut(s) 151
BstC8I GCNNGC 1 cut(s) 588
BstDEI CTNAG 4 cut(s) 196, 471, 798, 816
BstDSI CCRYGG 2 cut(s) 408, 862
BstF5I GGATG 3 cut(s) 112, 631, 637
BstKTI GATC 5 cut(s) 218, 222, 406, 778, 833
BstMAI GTCTC 2 cut(s) 794, 812
BstMBI GATC 5 cut(s) 215, 219, 403, 775, 830
BstMWI GCNNNNNNNGC 3 cut(s) 476, 722, 948
BstNI CCWGG 2 cut(s) 308, 481
BstNSI RCATGY 1 cut(s) 127
BstSCI CCNGG 2 cut(s) 306, 479
BstSLI GKGCMC 1 cut(s) 727
BstV2I GAAGAC 2 cut(s) 291, 669
BstX2I RGATCY 2 cut(s) 403, 830
BstYI RGATCY 2 cut(s) 403, 830
Bsu15I ATCGAT 1 cut(s) 218
BsuTUI ATCGAT 1 cut(s) 218
BtgI CCRYGG 2 cut(s) 408, 862
BtsCI GGATG 3 cut(s) 112, 631, 637
BtsI GCAGTG 2 cut(s) 725, 771
BtsIMutI CAGTG 3 cut(s) 725, 771, 922
Cac8I GCNNGC 1 cut(s) 588
ClaI ATCGAT 1 cut(s) 218
CseI GACGC 1 cut(s) 811
Csp6I GTAC 5 cut(s) 152, 192, 569, 670, 857
CviAII CATG 3 cut(s) 124, 409, 682
CviJI RGCY 9 cut(s) 34, 257, 313, 529, 593, 815, 905, 932, 951
CviKI_1 RGCY 9 cut(s) 34, 257, 313, 529, 593, 815, 905, 932, 951
CviQI GTAC 5 cut(s) 152, 192, 569, 670, 857
DdeI CTNAG 4 cut(s) 196, 471, 798, 816
DpnI GATC 5 cut(s) 217, 221, 405, 777, 832
DpnII GATC 5 cut(s) 215, 219, 403, 775, 830
Eam1104I CTCTTC 3 cut(s) 343, 501, 573
EarI CTCTTC 3 cut(s) 343, 501, 573
Eco130I CCWWGG 1 cut(s) 408
Eco24I GRGCYC 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 285
EcoRII CCWGG 2 cut(s) 306, 479
EcoT14I CCWWGG 1 cut(s) 408
EcoT38I GRGCYC 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 408
Esp3I CGTCTC 1 cut(s) 794
FaeI CATG 3 cut(s) 127, 412, 685
FalI AAGNNNNNCTT 2 cut(s) 480, 512
FatI CATG 3 cut(s) 123, 408, 681
Fnu4HI GCNGC 1 cut(s) 591
FokI GGATG 3 cut(s) 119, 624, 638
FriOI GRGCYC 1 cut(s) 36
Fsp4HI GCNGC 1 cut(s) 591
FspBI CTAG 3 cut(s) 14, 228, 344
GluI GCNGC 1 cut(s) 591
HgaI GACGC 1 cut(s) 811
Hin1II CATG 3 cut(s) 127, 412, 685
HinfI GANTC 8 cut(s) 17, 78, 179, 238, 260, 709, 908, 925
HphI GGTGA 3 cut(s) 528, 544, 763
Hpy166II GTNNAC 1 cut(s) 725
Hpy188I TCNGA 4 cut(s) 106, 199, 304, 421
Hpy188III TCNNGA 7 cut(s) 14, 129, 242, 264, 273, 523, 828
Hpy8I GTNNAC 1 cut(s) 725
HpyCH4III ACNGT 2 cut(s) 446, 573
HpyCH4IV ACGT 1 cut(s) 501
HpyF10VI GCNNNNNNNGC 3 cut(s) 476, 722, 948
HpyF3I CTNAG 4 cut(s) 196, 471, 798, 816
HpySE526I ACGT 1 cut(s) 501
Hsp92II CATG 3 cut(s) 127, 412, 685
Kzo9I GATC 5 cut(s) 215, 219, 403, 775, 830
LmnI GCTCC 3 cut(s) 583, 877, 948
LweI GCATC 3 cut(s) 127, 322, 646
MaeI CTAG 3 cut(s) 14, 228, 344
MaeII ACGT 1 cut(s) 501
MaeIII GTNAC 1 cut(s) 881
MalI GATC 5 cut(s) 217, 221, 405, 777, 832
MbiI CCGCTC 1 cut(s) 590
MboI GATC 5 cut(s) 215, 219, 403, 775, 830
MboII GAAGA 7 cut(s) 68, 291, 360, 488, 590, 669, 796
MflI RGATCY 2 cut(s) 403, 830
MhlI GDGCHC 4 cut(s) 36, 727, 771, 874
MluCI AATT 4 cut(s) 532, 557, 689, 823
MlyI GAGTC 3 cut(s) 247, 718, 934
MmeI TCCRAC 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 958
MseI TTAA 5 cut(s) 188, 224, 542, 692, 740
MslI CAYNNNNRTG 3 cut(s) 413, 665, 730
MspA1I CMGCKG 3 cut(s) 257, 905, 932
MspR9I CCNGG 2 cut(s) 308, 481
MvaI CCWGG 2 cut(s) 308, 481
MwoI GCNNNNNNNGC 3 cut(s) 476, 722, 948
NcoI CCATGG 1 cut(s) 408
NdeII GATC 5 cut(s) 215, 219, 403, 775, 830
NlaIII CATG 3 cut(s) 127, 412, 685
NlaIV GGNNCC 1 cut(s) 950
NmuCI GTSAC 1 cut(s) 881
NspI RCATGY 1 cut(s) 127
PciI ACATGT 1 cut(s) 123
PdmI GAANNNNTTC 1 cut(s) 958
PfeI GAWTC 5 cut(s) 17, 78, 179, 260, 908
PkrI GCNGC 1 cut(s) 592
PleI GAGTC 3 cut(s) 246, 717, 933
PpsI GAGTC 3 cut(s) 246, 717, 933
PscI ACATGT 1 cut(s) 123
PshBI ATTAAT 1 cut(s) 692
PsiI TTATAA 1 cut(s) 597
Psp6I CCWGG 2 cut(s) 306, 479
PspGI CCWGG 2 cut(s) 306, 479
PspN4I GGNNCC 1 cut(s) 950
PsuI RGATCY 2 cut(s) 403, 830
PvuII CAGCTG 3 cut(s) 257, 905, 932
RsaI GTAC 5 cut(s) 153, 193, 570, 671, 858
RsaNI GTAC 5 cut(s) 152, 192, 569, 670, 857
RseI CAYNNNNRTG 3 cut(s) 413, 665, 730
SaqAI TTAA 5 cut(s) 188, 224, 542, 692, 740
SatI GCNGC 1 cut(s) 591
Sau3AI GATC 5 cut(s) 215, 219, 403, 775, 830
ScaI AGTACT 1 cut(s) 570
SchI GAGTC 3 cut(s) 247, 718, 934
ScrFI CCNGG 2 cut(s) 308, 481
SduI GDGCHC 4 cut(s) 36, 727, 771, 874
SfaNI GCATC 3 cut(s) 127, 322, 646
SmiMI CAYNNNNRTG 3 cut(s) 413, 665, 730
Sse9I AATT 4 cut(s) 532, 557, 689, 823
SsiI CCGC 1 cut(s) 590
SspI AATATT 1 cut(s) 701
SspMI CTAG 3 cut(s) 14, 228, 344
StyD4I CCNGG 2 cut(s) 306, 479
StyI CCWWGG 1 cut(s) 408
TaaI ACNGT 2 cut(s) 446, 573
TaiI ACGT 1 cut(s) 504
TaqI TCGA 2 cut(s) 218, 696
TasI AATT 4 cut(s) 532, 557, 689, 823
TatI WGTACW 3 cut(s) 151, 568, 856
TauI GCSGC 1 cut(s) 593
TfiI GAWTC 5 cut(s) 17, 78, 179, 260, 908
Tru1I TTAA 5 cut(s) 188, 224, 542, 692, 740
Tru9I TTAA 5 cut(s) 188, 224, 542, 692, 740
TscAI CASTG 3 cut(s) 732, 771, 922
TseFI GTSAC 1 cut(s) 881
Tsp45I GTSAC 1 cut(s) 881
TspDTI ATGAA 4 cut(s) 591, 640, 765, 837
TspGWI ACGGA 2 cut(s) 851, 864
TspRI CASTG 3 cut(s) 732, 771, 922
VneI GTGCAC 1 cut(s) 723
VspI ATTAAT 1 cut(s) 692
XapI RAATTY 1 cut(s) 823
XbaI TCTAGA 1 cut(s) 13
XceI RCATGY 1 cut(s) 127
XmnI GAANNNNTTC 1 cut(s) 958
XspI CTAG 3 cut(s) 14, 228, 344
ZrmI AGTACT 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.