pycom10g08200

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011916
Physical Location & Seq
Forward (+)
10629889 .. 10630714
826 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 417 bp
ATGTCAAGATTTGAACTTTCATCATCTGCACCATACGACTCATCGTTGCCTCCTGAATATTCCTTCATGTCGATTTCTGGAATTCAAGGCCAGCTTCTTGAAGTCACTGTTGTTGGTTGTAACAAATTGAGAGATACGGAATGGATTTCTCGGCAGGACCCATATGTCTGTCTTGAATATGCTCACAACAAGCACCGCACCAGAACATGCACAGATGGTGGTAAAAATCCCACATTCCAAGAGAAGTTCGTGTTTCCGCTGATTGAAGGTCTTAGGGAACTCAATGTTCTGGTCTGGAATAGCAATACCGTCACATTGGACGACTTCATTGGCGAGGGAAAGGTTCAACTTCAGAAGGTTCTTACTCAGGGTTATGACGACAGCACATGGCAGCTTCAGACTAAACATGGCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04620 map04620
Pfam Domains
Protein Families
C2

Protein Analysis

139

Amino Acids

15.76

Weight (kDa)

5.57

Isoelectric Point (pI)

52.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 32 - 130 2.2e-16 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34150
fragaria_vesca FvH4_2g05480
malus_domestica MD00G1045300.v1.1 MD05G1087200.v1.1
prunus_persica Prupe.8G131800_v2.0.a1 Prupe.8G131800_v2.0.a1
pyrus_communis pycom05g08650 pycom10g08200
rosa_chinensis RchiOBHm_Chr6g0255751
rosa_laevigata RLG00000014816
rosa_multiflora Rmu_sc0001163.1_g000008 Rmu_sc0001163.1_g000009
rosa_roxburghii Rroxscaffold_7G00209700
rosa_rugosa Rorug05G0561000 Rorug05G0561100
rosa_samantha Rh6BG072200 Rh6CG070400 Rh6DG067600
rosa_wichuraiana Rw6G006820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
AciI CCGC 2 cut(s) 196, 257
AcoI YGGCCR 1 cut(s) 409
AcsI RAATTY 1 cut(s) 81
AcuI CTGAAG 2 cut(s) 335, 380
AgsI TTSAA 6 cut(s) 14, 86, 101, 176, 266, 347
AluBI AGCT 2 cut(s) 94, 394
AluI AGCT 2 cut(s) 94, 394
AoxI GGCC 2 cut(s) 88, 409
ApeKI GCWGC 1 cut(s) 391
ApoI RAATTY 1 cut(s) 81
AspS9I GGNCC 1 cut(s) 157
AvaII GGWCC 1 cut(s) 157
BbvI GCAGC 1 cut(s) 403
BccI CCATC 1 cut(s) 209
BisI GCNGC 1 cut(s) 392
BlsI GCNGC 1 cut(s) 393
Bme18I GGWCC 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 157
BmiI GGNNCC 1 cut(s) 159
Bse118I RCCGGY 1 cut(s) 411
BseMII CTCAG 1 cut(s) 380
BseXI GCAGC 1 cut(s) 403
BsgI GTGCAG 1 cut(s) 12
BshFI GGCC 2 cut(s) 90, 411
BsiSI CCGG 1 cut(s) 412
BsnI GGCC 2 cut(s) 90, 411
BspACI CCGC 2 cut(s) 196, 257
BspANI GGCC 2 cut(s) 90, 411
BspCNI CTCAG 1 cut(s) 379
BspLI GGNNCC 1 cut(s) 159
BsrFI RCCGGY 1 cut(s) 411
BssAI RCCGGY 1 cut(s) 411
Bst4CI ACNGT 2 cut(s) 109, 310
BstC8I GCNNGC 1 cut(s) 92
BstDEI CTNAG 2 cut(s) 272, 366
BstNSI RCATGY 1 cut(s) 210
BstV1I GCAGC 1 cut(s) 403
BsuRI GGCC 2 cut(s) 90, 411
BtsIMutI CAGTG 1 cut(s) 105
Cac8I GCNNGC 1 cut(s) 92
Cfr10I RCCGGY 1 cut(s) 411
Cfr13I GGNCC 1 cut(s) 157
CviAII CATG 4 cut(s) 67, 207, 387, 407
CviJI RGCY 4 cut(s) 90, 94, 394, 411
CviKI_1 RGCY 4 cut(s) 90, 94, 394, 411
DdeI CTNAG 2 cut(s) 272, 366
DrdI GACNNNNNNGTC 1 cut(s) 164
DseDI GACNNNNNNGTC 1 cut(s) 164
EaeI YGGCCR 1 cut(s) 409
Eco47I GGWCC 1 cut(s) 157
Eco57I CTGAAG 2 cut(s) 335, 380
EcoO109I RGGNCCY 1 cut(s) 157
EcoRI GAATTC 1 cut(s) 81
FaeI CATG 4 cut(s) 70, 210, 390, 410
FaiI YATR 9 cut(s) 34, 68, 163, 165, 180, 208, 375, 388, 408
FalI AAGNNNNNCTT 2 cut(s) 78, 110
FatI CATG 4 cut(s) 66, 206, 386, 406
FauNDI CATATG 1 cut(s) 163
Fnu4HI GCNGC 1 cut(s) 392
Fsp4HI GCNGC 1 cut(s) 392
GluI GCNGC 1 cut(s) 392
HaeIII GGCC 2 cut(s) 90, 411
HapII CCGG 1 cut(s) 412
Hin1II CATG 4 cut(s) 70, 210, 390, 410
HinfI GANTC 1 cut(s) 38
HpaII CCGG 1 cut(s) 412
Hpy188I TCNGA 2 cut(s) 354, 399
Hpy188III TCNNGA 6 cut(s) 6, 53, 78, 98, 173, 295
HpyAV CCTTC 3 cut(s) 73, 260, 349
HpyCH4III ACNGT 2 cut(s) 109, 310
HpyCH4V TGCA 2 cut(s) 29, 210
HpyF3I CTNAG 2 cut(s) 272, 366
Hsp92II CATG 4 cut(s) 70, 210, 390, 410
LpnPI CCDG 8 cut(s) 63, 66, 104, 140, 214, 275, 280, 353
Lsp1109I GCAGC 1 cut(s) 403
MaeIII GTNAC 3 cut(s) 103, 119, 310
MluCI AATT 2 cut(s) 81, 125
MlyI GAGTC 1 cut(s) 32
MnlI CCTC 2 cut(s) 60, 328
MspA1I CMGCKG 1 cut(s) 259
MspI CCGG 1 cut(s) 412
NdeI CATATG 1 cut(s) 163
NlaIII CATG 4 cut(s) 70, 210, 390, 410
NlaIV GGNNCC 1 cut(s) 159
NmeAIII GCCGAG 1 cut(s) 130
NmuCI GTSAC 2 cut(s) 103, 310
NspI RCATGY 1 cut(s) 210
PkrI GCNGC 1 cut(s) 393
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
PpuMI RGGWCCY 1 cut(s) 157
Psp5II RGGWCCY 1 cut(s) 157
PspN4I GGNNCC 1 cut(s) 159
PspPI GGNCC 1 cut(s) 157
PspPPI RGGWCCY 1 cut(s) 157
SatI GCNGC 1 cut(s) 392
Sau96I GGNCC 1 cut(s) 157
SchI GAGTC 1 cut(s) 32
SetI ASST 5 cut(s) 96, 271, 345, 360, 396
SinI GGWCC 1 cut(s) 157
Sse9I AATT 2 cut(s) 81, 125
SsiI CCGC 2 cut(s) 196, 257
SspI AATATT 1 cut(s) 59
TaaI ACNGT 2 cut(s) 109, 310
TaqI TCGA 1 cut(s) 71
TasI AATT 2 cut(s) 81, 125
TscAI CASTG 1 cut(s) 112
TseFI GTSAC 2 cut(s) 103, 310
TseI GCWGC 1 cut(s) 391
Tsp45I GTSAC 2 cut(s) 103, 310
TspDTI ATGAA 3 cut(s) 9, 55, 316
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 1 cut(s) 112
VpaK11BI GGWCC 1 cut(s) 157
XapI RAATTY 1 cut(s) 81
XceI RCATGY 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.