pycom10g09070
ERF Family

Radical SAM superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0007961
Physical Location & Seq
Forward (+)
11996084 .. 11997804
1721 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 909 bp
ATGGACTCCTCAGACCAGCTCACCACCAAGCTGCTCATAAAACTGCAGAATGGGGCTCTTGTGGAAGCTGTGATTATGCGATACGACAGCCGCTTGGGGAGGTATGATGGGAAGCCCCGCCCTGGTGGTCCTAGGTCCACCTTGTGTGTATCATCTCAGGTTGGTTGCAAAATGGGTTGTACTTTCTGTGCTACTGGGAGTATGGGGTTTAAGAGCAATCTGACCTCGGGGGAGATTGTGGAGCAGTTGGTTCATGCTTCTCGCATTGCGCCCATACGCAATGTTGTGTTCATGGGAATGGGGGAACCTTTGAATAATTATGGTTCCTTGGTGGAAGCGGTTCATGTCATGACTGGATCACCATTTCACTTGTCGCCTAAGAAAATTACTGTATCGACGGTTGGTATTATTCATGCTATCAAAAAGCTTCATAGTGATCTTCCAGGTCTGAATTTAGCAGTTTCACTGCATGCACCAGTCCAAGAAATCCGTTGTCAGATAATGCCTGCAGCTCGGGCTTTTCCTTTAGTAAAGCTTATGGATGCACTGCAAGAATATCAAAAGAACACTCAGCAGAAAATCTTCATTGAGTACATAATGCTTGATGGAGTGAATGATGAAGAGGACCATGCACACCTACTCGGAAAATTGCTAGAGACATTTCAAGTGGTAATTAATTTGATACCTTTTAATCCAATCGGTATTCTGAGTAAATTCACAACCAGTAACGAAAACAAAGTAACAAGATTCCAGAAAATTCTACGAGGCACTTATGGCATCCGAACGACAGTACGTAAGGAAATGGGTCAGGATATAAGTGGAGCATGTGGTCAGTTGGTAGTGAACTTACCCAACAAGAGGTCAACTGAGAACACTCTAACTGATATAGAAGATCTTCAGGTCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

303

Amino Acids

33.28

Weight (kDa)

8.98

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 51 - 212 4.4e-12 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 91, 118, 338
AclWI GGATC 1 cut(s) 364
AcsI RAATTY 3 cut(s) 451, 713, 756
AcuI CTGAAG 1 cut(s) 881
AdeI CACNNNGTG 1 cut(s) 144
AfaI GTAC 3 cut(s) 181, 593, 792
AfiI CCNNNNNNNGG 2 cut(s) 122, 858
AgsI TTSAA 2 cut(s) 313, 665
AjnI CCWGG 2 cut(s) 121, 442
AluBI AGCT 6 cut(s) 19, 31, 68, 427, 512, 535
AluI AGCT 6 cut(s) 19, 31, 68, 427, 512, 535
Alw26I GTCTC 1 cut(s) 650
AlwI GGATC 1 cut(s) 364
Ama87I CYCGRG 2 cut(s) 226, 513
ApeKI GCWGC 2 cut(s) 31, 509
ApoI RAATTY 3 cut(s) 451, 713, 756
AseI ATTAAT 1 cut(s) 675
Asp700I GAANNNNTTC 3 cut(s) 339, 581, 894
AspA2I CCTAGG 1 cut(s) 131
AspLEI GCGC 1 cut(s) 271
AspS9I GGNCC 3 cut(s) 128, 135, 625
AsuHPI GGTGA 2 cut(s) 13, 351
AvaI CYCGRG 2 cut(s) 226, 513
AvaII GGWCC 3 cut(s) 128, 135, 625
AvrII CCTAGG 1 cut(s) 131
BanII GRGCYC 1 cut(s) 58
BbvI GCAGC 2 cut(s) 18, 521
BccI CCATC 2 cut(s) 101, 599
BciT130I CCWGG 2 cut(s) 123, 444
BcoDI GTCTC 1 cut(s) 650
BfaI CTAG 2 cut(s) 132, 653
BfmI CTRYAG 2 cut(s) 44, 507
BglII AGATCT 1 cut(s) 892
BisI GCNGC 3 cut(s) 32, 91, 510
BlnI CCTAGG 1 cut(s) 131
BlsI GCNGC 3 cut(s) 33, 92, 511
Bme1390I CCNGG 2 cut(s) 123, 444
Bme18I GGWCC 3 cut(s) 128, 135, 625
BmeT110I CYCGRG 2 cut(s) 226, 513
BmgT120I GGNCC 3 cut(s) 128, 135, 625
BmiI GGNNCC 2 cut(s) 306, 325
BmrFI CCNGG 2 cut(s) 123, 444
BmrI ACTGGG 1 cut(s) 204
BmsI GCATC 2 cut(s) 532, 786
BmuI ACTGGG 1 cut(s) 204
BsaAI YACGTR 1 cut(s) 794
BsaJI CCNNGG 4 cut(s) 121, 131, 225, 327
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 858
Bse1I ACTGG 4 cut(s) 199, 358, 476, 723
Bse3DI GCAATG 2 cut(s) 264, 286
BseBI CCWGG 2 cut(s) 123, 444
BseDI CCNNGG 4 cut(s) 121, 131, 225, 327
BseGI GGATG 2 cut(s) 547, 777
BseLI CCNNNNNNNGG 2 cut(s) 122, 858
BseMI GCAATG 2 cut(s) 264, 286
BseMII CTCAG 5 cut(s) 24, 170, 584, 698, 858
BseNI ACTGG 4 cut(s) 199, 358, 476, 723
BseXI GCAGC 2 cut(s) 18, 521
BsiHKCI CYCGRG 2 cut(s) 226, 513
BslI CCNNNNNNNGG 2 cut(s) 122, 858
BsmAI GTCTC 1 cut(s) 650
BsoBI CYCGRG 2 cut(s) 226, 513
Bsp1286I GDGCHC 1 cut(s) 58
Bsp143I GATC 3 cut(s) 356, 436, 892
BspACI CCGC 3 cut(s) 91, 118, 338
BspCNI CTCAG 5 cut(s) 23, 169, 583, 699, 859
BspHI TCATGA 1 cut(s) 348
BspLI GGNNCC 2 cut(s) 306, 325
BspMAI CTGCAG 2 cut(s) 48, 511
BspPI GGATC 1 cut(s) 364
BsrDI GCAATG 2 cut(s) 264, 286
BsrI ACTGG 4 cut(s) 199, 358, 476, 723
BssECI CCNNGG 4 cut(s) 121, 131, 225, 327
BssMI GATC 3 cut(s) 356, 436, 892
BssT1I CCWWGG 2 cut(s) 131, 327
Bst2UI CCWGG 2 cut(s) 123, 444
Bst4CI ACNGT 3 cut(s) 391, 400, 790
Bst6I CTCTTC 1 cut(s) 615
BstBAI YACGTR 1 cut(s) 794
BstC8I GCNNGC 2 cut(s) 471, 507
BstDEI CTNAG 6 cut(s) 10, 156, 378, 570, 707, 867
BstF5I GGATG 2 cut(s) 547, 777
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 3 cut(s) 359, 439, 895
BstMAI GTCTC 1 cut(s) 650
BstMBI GATC 3 cut(s) 356, 436, 892
BstMWI GCNNNNNNNGC 2 cut(s) 515, 774
BstNI CCWGG 2 cut(s) 123, 444
BstNSI RCATGY 2 cut(s) 473, 828
BstSCI CCNGG 2 cut(s) 121, 442
BstSFI CTRYAG 2 cut(s) 44, 507
BstSNI TACGTA 1 cut(s) 794
BstV1I GCAGC 2 cut(s) 18, 521
BstX2I RGATCY 1 cut(s) 892
BstYI RGATCY 1 cut(s) 892
BtsCI GGATG 2 cut(s) 547, 777
BtsI GCAGTG 2 cut(s) 464, 545
BtsIMutI CAGTG 2 cut(s) 464, 545
Cac8I GCNNGC 2 cut(s) 471, 507
CciI TCATGA 1 cut(s) 348
CfoI GCGC 1 cut(s) 271
Cfr13I GGNCC 3 cut(s) 128, 135, 625
Csp6I GTAC 3 cut(s) 180, 592, 791
CviAII CATG 8 cut(s) 254, 292, 344, 349, 413, 470, 629, 825
CviQI GTAC 3 cut(s) 180, 592, 791
DdeI CTNAG 6 cut(s) 10, 156, 378, 570, 707, 867
DpnI GATC 3 cut(s) 358, 438, 894
DpnII GATC 3 cut(s) 356, 436, 892
DraIII CACNNNGTG 1 cut(s) 144
Eam1104I CTCTTC 1 cut(s) 615
EarI CTCTTC 1 cut(s) 615
Eco105I TACGTA 1 cut(s) 794
Eco130I CCWWGG 2 cut(s) 131, 327
Eco24I GRGCYC 1 cut(s) 58
Eco47I GGWCC 3 cut(s) 128, 135, 625
Eco57I CTGAAG 1 cut(s) 881
Eco88I CYCGRG 2 cut(s) 226, 513
EcoRII CCWGG 2 cut(s) 121, 442
EcoT14I CCWWGG 2 cut(s) 131, 327
EcoT38I GRGCYC 1 cut(s) 58
ErhI CCWWGG 2 cut(s) 131, 327
FaeI CATG 8 cut(s) 257, 295, 347, 352, 416, 473, 632, 828
FatI CATG 8 cut(s) 253, 291, 343, 348, 412, 469, 628, 824
FauI CCCGC 1 cut(s) 125
Fnu4HI GCNGC 3 cut(s) 32, 91, 510
FokI GGATG 2 cut(s) 554, 764
FriOI GRGCYC 1 cut(s) 58
Fsp4HI GCNGC 3 cut(s) 32, 91, 510
FspBI CTAG 2 cut(s) 132, 653
GlaI GCGC 1 cut(s) 270
GluI GCNGC 3 cut(s) 32, 91, 510
HhaI GCGC 1 cut(s) 271
Hin1II CATG 8 cut(s) 257, 295, 347, 352, 416, 473, 632, 828
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HincII GTYRAC 1 cut(s) 864
HindII GTYRAC 1 cut(s) 864
HindIII AAGCTT 2 cut(s) 425, 533
HinfI GANTC 2 cut(s) 5, 747
HphI GGTGA 2 cut(s) 13, 351
Hpy166II GTNNAC 3 cut(s) 138, 844, 864
Hpy188I TCNGA 8 cut(s) 13, 222, 450, 498, 644, 708, 782, 905
Hpy188III TCNNGA 3 cut(s) 349, 751, 809
Hpy8I GTNNAC 3 cut(s) 138, 844, 864
Hpy99I CGWCG 1 cut(s) 400
HpyCH4III ACNGT 3 cut(s) 391, 400, 790
HpyCH4IV ACGT 1 cut(s) 793
HpyCH4V TGCA 8 cut(s) 46, 168, 469, 473, 509, 545, 550, 632
HpyF10VI GCNNNNNNNGC 2 cut(s) 515, 774
HpyF3I CTNAG 6 cut(s) 10, 156, 378, 570, 707, 867
HpySE526I ACGT 1 cut(s) 793
Hsp92II CATG 8 cut(s) 257, 295, 347, 352, 416, 473, 632, 828
HspAI GCGC 1 cut(s) 269
Kzo9I GATC 3 cut(s) 356, 436, 892
LmnI GCTCC 2 cut(s) 241, 821
Lsp1109I GCAGC 2 cut(s) 18, 521
LweI GCATC 2 cut(s) 532, 786
MaeI CTAG 2 cut(s) 132, 653
MaeII ACGT 1 cut(s) 793
MaeIII GTNAC 2 cut(s) 725, 739
MalI GATC 3 cut(s) 358, 438, 894
MboI GATC 3 cut(s) 356, 436, 892
MboII GAAGA 5 cut(s) 431, 574, 632, 887, 902
MflI RGATCY 1 cut(s) 892
MhlI GDGCHC 1 cut(s) 58
MluCI AATT 8 cut(s) 316, 384, 451, 647, 672, 676, 713, 756
MnlI CCTC 6 cut(s) 19, 93, 235, 616, 758, 852
MroXI GAANNNNTTC 3 cut(s) 339, 581, 894
MseI TTAA 3 cut(s) 210, 675, 690
MslI CAYNNNNRTG 1 cut(s) 296
MspR9I CCNGG 2 cut(s) 123, 444
MvaI CCWGG 2 cut(s) 123, 444
MwoI GCNNNNNNNGC 2 cut(s) 515, 774
NdeII GATC 3 cut(s) 356, 436, 892
NlaIII CATG 8 cut(s) 257, 295, 347, 352, 416, 473, 632, 828
NlaIV GGNNCC 2 cut(s) 306, 325
NspI RCATGY 2 cut(s) 473, 828
PaeI GCATGC 1 cut(s) 473
PagI TCATGA 1 cut(s) 348
PdmI GAANNNNTTC 3 cut(s) 339, 581, 894
PfeI GAWTC 1 cut(s) 747
PkrI GCNGC 3 cut(s) 33, 92, 511
Ppu21I YACGTR 1 cut(s) 794
PshBI ATTAAT 1 cut(s) 675
Psp6I CCWGG 2 cut(s) 121, 442
PspGI CCWGG 2 cut(s) 121, 442
PspN4I GGNNCC 2 cut(s) 306, 325
PspPI GGNCC 3 cut(s) 128, 135, 625
PstI CTGCAG 2 cut(s) 48, 511
PsuI RGATCY 1 cut(s) 892
RsaI GTAC 3 cut(s) 181, 593, 792
RsaNI GTAC 3 cut(s) 180, 592, 791
RseI CAYNNNNRTG 1 cut(s) 296
SaqAI TTAA 3 cut(s) 210, 675, 690
SatI GCNGC 3 cut(s) 32, 91, 510
Sau3AI GATC 3 cut(s) 356, 436, 892
Sau96I GGNCC 3 cut(s) 128, 135, 625
ScrFI CCNGG 2 cut(s) 123, 444
SduI GDGCHC 1 cut(s) 58
SfaNI GCATC 2 cut(s) 532, 786
SfcI CTRYAG 2 cut(s) 44, 507
SinI GGWCC 3 cut(s) 128, 135, 625
SmiMI CAYNNNNRTG 1 cut(s) 296
SnaBI TACGTA 1 cut(s) 794
SphI GCATGC 1 cut(s) 473
Sse9I AATT 8 cut(s) 316, 384, 451, 647, 672, 676, 713, 756
SsiI CCGC 3 cut(s) 91, 118, 338
SspMI CTAG 2 cut(s) 132, 653
StyD4I CCNGG 2 cut(s) 121, 442
StyI CCWWGG 2 cut(s) 131, 327
TaaI ACNGT 3 cut(s) 391, 400, 790
TaiI ACGT 1 cut(s) 796
TaqI TCGA 1 cut(s) 395
TasI AATT 8 cut(s) 316, 384, 451, 647, 672, 676, 713, 756
TatI WGTACW 2 cut(s) 179, 591
TauI GCSGC 1 cut(s) 93
TfiI GAWTC 1 cut(s) 747
Tru1I TTAA 3 cut(s) 210, 675, 690
Tru9I TTAA 3 cut(s) 210, 675, 690
TscAI CASTG 2 cut(s) 471, 552
TseI GCWGC 2 cut(s) 31, 509
TspDTI ATGAA 7 cut(s) 242, 280, 332, 401, 419, 574, 633
TspGWI ACGGA 1 cut(s) 479
TspRI CASTG 2 cut(s) 471, 552
VpaK11BI GGWCC 3 cut(s) 128, 135, 625
VspI ATTAAT 1 cut(s) 675
XapI RAATTY 3 cut(s) 451, 713, 756
XceI RCATGY 2 cut(s) 473, 828
XmaJI CCTAGG 1 cut(s) 131
XmnI GAANNNNTTC 3 cut(s) 339, 581, 894
XspI CTAG 2 cut(s) 132, 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.