pycom10g12510

Tetraketide alpha-pyrone reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008638
Physical Location & Seq
Reverse (-)
15985036 .. 15986351
1316 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 696 bp
ATGCACATACAGAGAGAAATCCTGGAGCCAGCGATTGAGGGTACCTTGAACGTGTTGCGTTCGTGTAAGAAAAATCCGTCTCTGAGGCGAGTGGTTTTCACTTCATCTTCTTCAACTCTAAGGGTGAAACCGGATGAAGATTTCGCCTCCAGTTTAAAACTGGATGAGTCATCTTGGAGCTCTGTGGAATACTGCCAAAGACTTCAGCTCTGGTACCCTTTGTCAAAAGTTCTAGCCGAGAAGGCAGCAGGGGATTTTTGCAAAGAGAATGGGATCGATTTAGTAACTGTTATACCTGCGTGGTTGATTGGACCTAGTTTGTCACCTGATCTATCTCATACTGCATCGCGGGTTCTCAGCCTGCTTAAAGGCGATACAGTATGGTATGGAAGAATGGGATATGTTCACATTGATGATGTTGCACTCTGTCACATCTTAGTTTATGAGCATGAAAGTGCTCATGGTCGATATCTTTGTAGCTCAACAGTTTTGGACAACGATGAGTTGGCTTCCTTATTATCCATGCGATATCCTTCCTTACCTATTGCCAAAAGTTTGAAGCAAGTAAGCAGACCATATTATGAGTTCGACACGTCAAAGTTGCAGAGTCTAGGATTCAAGTTCAAGACTATCCAGGAGATGTTTGATGATTGCATTGACTCTTTGTTGGAGCAAGGCCATCTTTCTGCACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase UFD1

Protein Analysis

232

Amino Acids

26.02

Weight (kDa)

5.8

Isoelectric Point (pI)

49.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 7 - 153 2.7e-10 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 304
Acc65I GGTACC 2 cut(s) 41, 213
AccB1I GGYRCC 2 cut(s) 41, 213
AccII CGCG 1 cut(s) 349
AciI CCGC 1 cut(s) 349
AclWI GGATC 1 cut(s) 281
AcuI CTGAAG 1 cut(s) 188
AfaI GTAC 2 cut(s) 43, 215
AflIII ACRYGT 2 cut(s) 51, 591
AgsI TTSAA 5 cut(s) 49, 114, 559, 619, 625
AjiI CACGTC 1 cut(s) 594
AjnI CCWGG 2 cut(s) 21, 633
AluBI AGCT 3 cut(s) 180, 208, 480
AluI AGCT 3 cut(s) 180, 208, 480
Alw21I GWGCWC 2 cut(s) 182, 460
Alw26I GTCTC 1 cut(s) 84
AlwI GGATC 1 cut(s) 281
AoxI GGCC 1 cut(s) 676
ApeKI GCWGC 1 cut(s) 245
Asp718I GGTACC 2 cut(s) 41, 213
AspS9I GGNCC 1 cut(s) 311
AsuHPI GGTGA 2 cut(s) 136, 315
AvaII GGWCC 1 cut(s) 311
BanI GGYRCC 2 cut(s) 41, 213
BanII GRGCYC 1 cut(s) 182
Bbv12I GWGCWC 2 cut(s) 182, 460
BbvI GCAGC 1 cut(s) 257
BccI CCATC 1 cut(s) 687
BciT130I CCWGG 2 cut(s) 23, 635
BcoDI GTCTC 1 cut(s) 84
BfaI CTAG 4 cut(s) 233, 315, 611, 694
BfuAI ACCTGC 1 cut(s) 304
BglI GCCNNNNNGGC 1 cut(s) 242
BisI GCNGC 1 cut(s) 246
BlsI GCNGC 1 cut(s) 247
Bme1390I CCNGG 2 cut(s) 23, 635
Bme18I GGWCC 1 cut(s) 311
BmgBI CACGTC 1 cut(s) 594
BmgT120I GGNCC 1 cut(s) 311
BmiI GGNNCC 3 cut(s) 27, 43, 215
BmrFI CCNGG 2 cut(s) 23, 635
BmsI GCATC 1 cut(s) 353
BpmI CTGGAG 2 cut(s) 44, 133
Bsa29I ATCGAT 1 cut(s) 276
BsaWI WCCGGW 1 cut(s) 130
Bse1I ACTGG 2 cut(s) 150, 165
BseBI CCWGG 2 cut(s) 23, 635
BseCI ATCGAT 1 cut(s) 276
BseGI GGATG 2 cut(s) 139, 169
BseMII CTCAG 2 cut(s) 74, 370
BseNI ACTGG 2 cut(s) 150, 165
BseXI GCAGC 1 cut(s) 257
BsgI GTGCAG 1 cut(s) 672
Bsh1236I CGCG 1 cut(s) 349
BshFI GGCC 1 cut(s) 678
BshNI GGYRCC 2 cut(s) 41, 213
BshVI ATCGAT 1 cut(s) 276
BsiHKAI GWGCWC 2 cut(s) 182, 460
BsiSI CCGG 1 cut(s) 131
BsmAI GTCTC 1 cut(s) 84
BsmBI CGTCTC 1 cut(s) 84
BsnI GGCC 1 cut(s) 678
Bsp1286I GDGCHC 2 cut(s) 182, 460
Bsp143I GATC 2 cut(s) 273, 328
BspACI CCGC 1 cut(s) 349
BspANI GGCC 1 cut(s) 678
BspCNI CTCAG 2 cut(s) 75, 369
BspDI ATCGAT 1 cut(s) 276
BspFNI CGCG 1 cut(s) 349
BspLI GGNNCC 3 cut(s) 27, 43, 215
BspMI ACCTGC 1 cut(s) 304
BspPI GGATC 1 cut(s) 281
BspT107I GGYRCC 2 cut(s) 41, 213
BsrI ACTGG 2 cut(s) 150, 165
BssMI GATC 2 cut(s) 273, 328
Bst2UI CCWGG 2 cut(s) 23, 635
Bst4CI ACNGT 3 cut(s) 289, 379, 487
BstC8I GCNNGC 2 cut(s) 30, 362
BstDEI CTNAG 4 cut(s) 83, 119, 356, 436
BstF5I GGATG 2 cut(s) 139, 169
BstFNI CGCG 1 cut(s) 349
BstKTI GATC 2 cut(s) 276, 331
BstMAI GTCTC 1 cut(s) 84
BstMBI GATC 2 cut(s) 273, 328
BstMWI GCNNNNNNNGC 1 cut(s) 242
BstNI CCWGG 2 cut(s) 23, 635
BstSCI CCNGG 2 cut(s) 21, 633
BstUI CGCG 1 cut(s) 349
BstV1I GCAGC 1 cut(s) 257
Bsu15I ATCGAT 1 cut(s) 276
BsuRI GGCC 1 cut(s) 678
BsuTUI ATCGAT 1 cut(s) 276
BtgZI GCGATG 1 cut(s) 330
BtrI CACGTC 1 cut(s) 594
BtsCI GGATG 2 cut(s) 139, 169
BveI ACCTGC 1 cut(s) 304
Cac8I GCNNGC 2 cut(s) 30, 362
Cfr13I GGNCC 1 cut(s) 311
ClaI ATCGAT 1 cut(s) 276
Csp6I GTAC 2 cut(s) 42, 214
CviAII CATG 3 cut(s) 449, 461, 523
CviJI RGCY 8 cut(s) 28, 180, 208, 236, 360, 480, 509, 678
CviKI_1 RGCY 8 cut(s) 28, 180, 208, 236, 360, 480, 509, 678
CviQI GTAC 2 cut(s) 42, 214
DdeI CTNAG 4 cut(s) 83, 119, 356, 436
DpnI GATC 2 cut(s) 275, 330
DpnII GATC 2 cut(s) 273, 328
DraI TTTAAA 1 cut(s) 156
Ecl136II GAGCTC 1 cut(s) 180
Eco24I GRGCYC 1 cut(s) 182
Eco32I GATATC 2 cut(s) 470, 530
Eco47I GGWCC 1 cut(s) 311
Eco53kI GAGCTC 1 cut(s) 180
Eco57I CTGAAG 1 cut(s) 188
EcoICRI GAGCTC 1 cut(s) 180
EcoRII CCWGG 2 cut(s) 21, 633
EcoRV GATATC 2 cut(s) 470, 530
EcoT38I GRGCYC 1 cut(s) 182
Esp3I CGTCTC 1 cut(s) 84
FaeI CATG 3 cut(s) 452, 464, 526
FalI AAGNNNNNCTT 1 cut(s) 666
FatI CATG 3 cut(s) 448, 460, 522
FauI CCCGC 1 cut(s) 342
Fnu4HI GCNGC 1 cut(s) 246
FokI GGATG 2 cut(s) 146, 176
FriOI GRGCYC 1 cut(s) 182
Fsp4HI GCNGC 1 cut(s) 246
FspBI CTAG 4 cut(s) 233, 315, 611, 694
GluI GCNGC 1 cut(s) 246
GsuI CTGGAG 2 cut(s) 44, 133
HaeIII GGCC 1 cut(s) 678
HapII CCGG 1 cut(s) 131
Hin1II CATG 3 cut(s) 452, 464, 526
HinfI GANTC 4 cut(s) 167, 607, 615, 659
HpaII CCGG 1 cut(s) 131
HphI GGTGA 2 cut(s) 136, 315
Hpy166II GTNNAC 1 cut(s) 406
Hpy188I TCNGA 1 cut(s) 84
Hpy188III TCNNGA 1 cut(s) 625
Hpy8I GTNNAC 1 cut(s) 406
HpyAV CCTTC 2 cut(s) 235, 543
HpyCH4III ACNGT 3 cut(s) 289, 379, 487
HpyCH4IV ACGT 2 cut(s) 51, 593
HpyCH4V TGCA 7 cut(s) 4, 261, 344, 422, 604, 654, 689
HpyF10VI GCNNNNNNNGC 1 cut(s) 242
HpyF3I CTNAG 4 cut(s) 83, 119, 356, 436
HpySE526I ACGT 2 cut(s) 51, 593
Hsp92II CATG 3 cut(s) 452, 464, 526
KpnI GGTACC 2 cut(s) 45, 217
Kzo9I GATC 2 cut(s) 273, 328
LmnI GCTCC 3 cut(s) 25, 177, 670
Lsp1109I GCAGC 1 cut(s) 257
LweI GCATC 1 cut(s) 353
MaeI CTAG 4 cut(s) 233, 315, 611, 694
MaeII ACGT 2 cut(s) 51, 593
MaeIII GTNAC 3 cut(s) 283, 321, 428
MalI GATC 2 cut(s) 275, 330
MboI GATC 2 cut(s) 273, 328
MboII GAAGA 4 cut(s) 99, 102, 149, 402
MhlI GDGCHC 2 cut(s) 182, 460
MlyI GAGTC 3 cut(s) 176, 616, 653
MmeI TCCRAC 1 cut(s) 648
MnlI CCTC 3 cut(s) 31, 78, 157
MseI TTAA 2 cut(s) 155, 366
MslI CAYNNNNRTG 2 cut(s) 411, 453
MspI CCGG 1 cut(s) 131
MspR9I CCNGG 2 cut(s) 23, 635
MvaI CCWGG 2 cut(s) 23, 635
MvnI CGCG 1 cut(s) 349
MwoI GCNNNNNNNGC 1 cut(s) 242
NdeII GATC 2 cut(s) 273, 328
NlaIII CATG 3 cut(s) 452, 464, 526
NlaIV GGNNCC 3 cut(s) 27, 43, 215
NmeAIII GCCGAG 1 cut(s) 262
NmuCI GTSAC 2 cut(s) 321, 428
PfeI GAWTC 1 cut(s) 615
PfoI TCCNGGA 2 cut(s) 21, 633
PkrI GCNGC 1 cut(s) 247
PleI GAGTC 3 cut(s) 175, 615, 653
PpsI GAGTC 3 cut(s) 175, 615, 653
Psp124BI GAGCTC 1 cut(s) 182
Psp6I CCWGG 2 cut(s) 21, 633
PspGI CCWGG 2 cut(s) 21, 633
PspN4I GGNNCC 3 cut(s) 27, 43, 215
PspPI GGNCC 1 cut(s) 311
RsaI GTAC 2 cut(s) 43, 215
RsaNI GTAC 2 cut(s) 42, 214
RseI CAYNNNNRTG 2 cut(s) 411, 453
SacI GAGCTC 1 cut(s) 182
SaqAI TTAA 2 cut(s) 155, 366
SatI GCNGC 1 cut(s) 246
Sau3AI GATC 2 cut(s) 273, 328
Sau96I GGNCC 1 cut(s) 311
SchI GAGTC 3 cut(s) 176, 616, 653
ScrFI CCNGG 2 cut(s) 23, 635
SduI GDGCHC 2 cut(s) 182, 460
SfaNI GCATC 1 cut(s) 353
SinI GGWCC 1 cut(s) 311
SmiMI CAYNNNNRTG 2 cut(s) 411, 453
SsiI CCGC 1 cut(s) 349
SspMI CTAG 4 cut(s) 233, 315, 611, 694
SstI GAGCTC 1 cut(s) 182
StyD4I CCNGG 2 cut(s) 21, 633
TaaI ACNGT 3 cut(s) 289, 379, 487
TaiI ACGT 2 cut(s) 54, 596
TaqI TCGA 3 cut(s) 276, 466, 588
TfiI GAWTC 1 cut(s) 615
Tru1I TTAA 2 cut(s) 155, 366
Tru9I TTAA 2 cut(s) 155, 366
TseFI GTSAC 2 cut(s) 321, 428
TseI GCWGC 1 cut(s) 245
Tsp45I GTSAC 2 cut(s) 321, 428
TspDTI ATGAA 3 cut(s) 93, 150, 465
TspGWI ACGGA 1 cut(s) 66
VpaK11BI GGWCC 1 cut(s) 311
XcmI CCANNNNNNNNNTGG 1 cut(s) 157
XspI CTAG 4 cut(s) 233, 315, 611, 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.