pycom10g12920

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010105
Physical Location & Seq
Forward (+)
16398049 .. 16398721
673 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 576 bp
ATGGATGAATCTGACGGCTACCCAAAAGAGTGCTTCACCCAAGACGGCCGGAGAGTGATGTCGTCATCGTCAACGACGAGCGTGCACGTCACGGCGCTGGACGGCCTCGTCAACGTCAACTCACTCTTCACCATCGCCGTCTTCGTGGGGCTCTCCCTGACAACTCGGGGACAGAAGAGCCTCGAGAACCGGACATCCTGCGACGCCGGGATCGATGTGGCGAAGAAGCTGCTGGTGTTCGAGGTTGTCTCCTTCAGCTTCTTCCTGTTCTCGTCGCTGGTGGCGCAGGGACTGAAGCTGGCGATCAACCTGCTCAACAGCAAGGACGTCGACGAGGCGTTCCGGGCCCACATCAATCTGAAGGCGTTGAGATTCGGGATGTTGGCGTCCGCTTTCGGATCGGTCATGGGTTGTGTGTTTTTGGTGCTGTCGATGGTGAATGTGATTCAAATCAGGCTGGGGATGCTGTCGTGTGGCAGCAAACCCGCCGTCCATTCCGTGGCGGCGCTTGTTGTATTGGTCACGACGGCGCTTCTGGTCTATATTTCCACTGCTGTTTATGCTTTTCTTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

192

Amino Acids

20.49

Weight (kDa)

8.39

Isoelectric Point (pI)

26.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 107
AatII GACGTC 1 cut(s) 330
Acc36I ACCTGC 1 cut(s) 318
AccB7I CCANNNNNTGG 1 cut(s) 499
AccI GTMKAC 1 cut(s) 330
AciI CCGC 3 cut(s) 390, 486, 503
AclWI GGATC 2 cut(s) 218, 406
AcoI YGGCCR 1 cut(s) 46
AcuI CTGAAG 3 cut(s) 238, 314, 380
AcyI GRCGYC 3 cut(s) 204, 327, 386
AfiI CCNNNNNNNGG 1 cut(s) 499
AgsI TTSAA 1 cut(s) 449
AjiI CACGTC 1 cut(s) 88
AloI GAACNNNNNNTCC 2 cut(s) 179, 211
AluBI AGCT 3 cut(s) 229, 258, 298
AluI AGCT 3 cut(s) 229, 258, 298
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 1 cut(s) 253
Alw44I GTGCAC 1 cut(s) 83
AlwI GGATC 2 cut(s) 218, 406
AlwNI CAGNNNCTG 1 cut(s) 292
Ama87I CYCGRG 2 cut(s) 165, 182
AoxI GGCC 3 cut(s) 46, 103, 345
ApaI GGGCCC 1 cut(s) 349
ApaLI GTGCAC 1 cut(s) 83
ApeKI GCWGC 2 cut(s) 229, 477
ArsI GACNNNNNNTTYG 2 cut(s) 474, 506
AspLEI GCGC 4 cut(s) 97, 286, 508, 532
AspS9I GGNCC 2 cut(s) 345, 346
AsuC2I CCSGG 2 cut(s) 208, 344
AsuHPI GGTGA 3 cut(s) 28, 121, 448
AvaI CYCGRG 2 cut(s) 165, 182
BaeGI GKGCMC 2 cut(s) 87, 349
BanII GRGCYC 2 cut(s) 153, 349
BbsI GAAGAC 1 cut(s) 133
Bbv12I GWGCWC 1 cut(s) 87
BbvI GCAGC 2 cut(s) 216, 489
BccI CCATC 2 cut(s) 140, 427
BceAI ACGGC 7 cut(s) 31, 61, 108, 118, 122, 473, 543
BcgI CGANNNNNNTGC 8 cut(s) 64, 98, 211, 245, 292, 310, 326, 344
BcnI CCSGG 2 cut(s) 208, 344
BcoDI GTCTC 1 cut(s) 253
BfaI CTAG 1 cut(s) 574
BfoI RGCGCY 3 cut(s) 98, 509, 533
BfuAI ACCTGC 1 cut(s) 318
BisI GCNGC 3 cut(s) 230, 478, 504
BlsI GCNGC 3 cut(s) 231, 479, 505
Bme1390I CCNGG 2 cut(s) 208, 344
BmeT110I CYCGRG 2 cut(s) 165, 182
BmgBI CACGTC 1 cut(s) 88
BmgT120I GGNCC 2 cut(s) 345, 346
BmiI GGNNCC 1 cut(s) 347
BmrFI CCNGG 2 cut(s) 208, 344
BmsI GCATC 1 cut(s) 453
BpiI GAAGAC 1 cut(s) 133
BplI GAGNNNNNCTC 2 cut(s) 233, 265
BpuMI CCSGG 2 cut(s) 208, 344
Bsa29I ATCGAT 1 cut(s) 213
BsaBI GATNNNNATC 1 cut(s) 449
BsaHI GRCGYC 3 cut(s) 204, 327, 386
BsaJI CCNNGG 1 cut(s) 498
BsaWI WCCGGW 1 cut(s) 189
Bsc4I CCNNNNNNNGG 1 cut(s) 499
Bse8I GATNNNNATC 1 cut(s) 449
BseCI ATCGAT 1 cut(s) 213
BseDI CCNNGG 1 cut(s) 498
BseGI GGATG 4 cut(s) 10, 194, 384, 468
BseJI GATNNNNATC 1 cut(s) 449
BseLI CCNNNNNNNGG 1 cut(s) 499
BseSI GKGCMC 2 cut(s) 87, 349
BseX3I CGGCCG 1 cut(s) 46
BseXI GCAGC 2 cut(s) 216, 489
BseYI CCCAGC 1 cut(s) 457
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 3 cut(s) 48, 105, 347
BshVI ATCGAT 1 cut(s) 213
BsiEI CGRYCG 1 cut(s) 49
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 2 cut(s) 165, 182
BsiSI CCGG 4 cut(s) 49, 190, 207, 343
BslFI GGGAC 2 cut(s) 183, 303
BslI CCNNNNNNNGG 1 cut(s) 499
BsmAI GTCTC 1 cut(s) 253
BsmFI GGGAC 2 cut(s) 183, 303
BsnI GGCC 3 cut(s) 48, 105, 347
BsoBI CYCGRG 2 cut(s) 165, 182
Bsp120I GGGCCC 1 cut(s) 345
Bsp1286I GDGCHC 3 cut(s) 87, 153, 349
Bsp143I GATC 3 cut(s) 210, 303, 398
BspACI CCGC 3 cut(s) 390, 486, 503
BspANI GGCC 3 cut(s) 48, 105, 347
BspDI ATCGAT 1 cut(s) 213
BspLI GGNNCC 1 cut(s) 347
BspMI ACCTGC 1 cut(s) 318
BspPI GGATC 2 cut(s) 218, 406
BspQI GCTCTTC 1 cut(s) 170
BssECI CCNNGG 1 cut(s) 498
BssMI GATC 3 cut(s) 210, 303, 398
BssNI GRCGYC 3 cut(s) 204, 327, 386
Bst6I CTCTTC 2 cut(s) 131, 170
BstACI GRCGYC 3 cut(s) 204, 327, 386
BstC8I GCNNGC 2 cut(s) 83, 300
BstDSI CCRYGG 1 cut(s) 498
BstF5I GGATG 4 cut(s) 10, 194, 384, 468
BstH2I RGCGCY 3 cut(s) 98, 509, 533
BstHHI GCGC 4 cut(s) 97, 286, 508, 532
BstKTI GATC 3 cut(s) 213, 306, 401
BstMAI GTCTC 1 cut(s) 253
BstMBI GATC 3 cut(s) 210, 303, 398
BstMCI CGRYCG 1 cut(s) 49
BstMWI GCNNNNNNNGC 4 cut(s) 283, 344, 463, 560
BstSCI CCNGG 2 cut(s) 206, 342
BstSLI GKGCMC 2 cut(s) 87, 349
BstV1I GCAGC 2 cut(s) 216, 489
BstV2I GAAGAC 1 cut(s) 133
BstZI CGGCCG 1 cut(s) 46
Bsu15I ATCGAT 1 cut(s) 213
BsuRI GGCC 3 cut(s) 48, 105, 347
BsuTUI ATCGAT 1 cut(s) 213
BtgI CCRYGG 1 cut(s) 498
BtgZI GCGATG 1 cut(s) 118
BtrI CACGTC 1 cut(s) 88
BtsCI GGATG 4 cut(s) 10, 194, 384, 468
BtsI GCAGTG 1 cut(s) 549
BtsIMutI CAGTG 1 cut(s) 549
BveI ACCTGC 1 cut(s) 318
Cac8I GCNNGC 2 cut(s) 83, 300
CaiI CAGNNNCTG 1 cut(s) 292
CfoI GCGC 4 cut(s) 97, 286, 508, 532
Cfr13I GGNCC 2 cut(s) 345, 346
ClaI ATCGAT 1 cut(s) 213
CseI GACGC 2 cut(s) 212, 375
CviAII CATG 1 cut(s) 406
DpnI GATC 3 cut(s) 212, 305, 400
DpnII GATC 3 cut(s) 210, 303, 398
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
EaeI YGGCCR 1 cut(s) 46
EagI CGGCCG 1 cut(s) 46
Eam1104I CTCTTC 2 cut(s) 131, 170
EarI CTCTTC 2 cut(s) 131, 170
EclXI CGGCCG 1 cut(s) 46
Eco24I GRGCYC 2 cut(s) 153, 349
Eco52I CGGCCG 1 cut(s) 46
Eco57I CTGAAG 3 cut(s) 238, 314, 380
Eco88I CYCGRG 2 cut(s) 165, 182
EcoT38I GRGCYC 2 cut(s) 153, 349
FaeI CATG 1 cut(s) 409
FaiI YATR 3 cut(s) 407, 543, 561
FaqI GGGAC 2 cut(s) 183, 303
FatI CATG 1 cut(s) 405
FauI CCCGC 1 cut(s) 493
FblI GTMKAC 1 cut(s) 330
Fnu4HI GCNGC 3 cut(s) 230, 478, 504
FokI GGATG 4 cut(s) 17, 181, 391, 475
FriOI GRGCYC 2 cut(s) 153, 349
Fsp4HI GCNGC 3 cut(s) 230, 478, 504
FspBI CTAG 1 cut(s) 574
GlaI GCGC 4 cut(s) 96, 285, 507, 531
GluI GCNGC 3 cut(s) 230, 478, 504
GsaI CCCAGC 1 cut(s) 461
HaeII RGCGCY 3 cut(s) 98, 509, 533
HaeIII GGCC 3 cut(s) 48, 105, 347
HapII CCGG 4 cut(s) 49, 190, 207, 343
HgaI GACGC 2 cut(s) 212, 375
HhaI GCGC 4 cut(s) 97, 286, 508, 532
Hin1I GRCGYC 3 cut(s) 204, 327, 386
Hin1II CATG 1 cut(s) 409
Hin6I GCGC 4 cut(s) 95, 284, 506, 530
HinP1I GCGC 4 cut(s) 95, 284, 506, 530
HincII GTYRAC 4 cut(s) 72, 112, 118, 331
HindII GTYRAC 4 cut(s) 72, 112, 118, 331
HinfI GANTC 3 cut(s) 8, 372, 445
HpaII CCGG 4 cut(s) 49, 190, 207, 343
HphI GGTGA 3 cut(s) 28, 121, 448
Hpy166II GTNNAC 5 cut(s) 72, 85, 112, 118, 331
Hpy188I TCNGA 3 cut(s) 13, 360, 398
Hpy188III TCNNGA 3 cut(s) 184, 376, 523
Hpy8I GTNNAC 5 cut(s) 72, 85, 112, 118, 331
Hpy99I CGWCG 6 cut(s) 79, 206, 277, 332, 335, 529
HpyAV CCTTC 2 cut(s) 262, 355
HpyCH4IV ACGT 3 cut(s) 87, 114, 327
HpyCH4V TGCA 1 cut(s) 85
HpyF10VI GCNNNNNNNGC 4 cut(s) 283, 344, 463, 560
HpySE526I ACGT 3 cut(s) 87, 114, 327
Hsp92I GRCGYC 3 cut(s) 204, 327, 386
Hsp92II CATG 1 cut(s) 409
HspAI GCGC 4 cut(s) 95, 284, 506, 530
Kzo9I GATC 3 cut(s) 210, 303, 398
LguI GCTCTTC 1 cut(s) 170
Lsp1109I GCAGC 2 cut(s) 216, 489
LweI GCATC 1 cut(s) 453
MaeI CTAG 1 cut(s) 574
MaeII ACGT 3 cut(s) 87, 114, 327
MaeIII GTNAC 2 cut(s) 88, 520
MalI GATC 3 cut(s) 212, 305, 400
MboI GATC 3 cut(s) 210, 303, 398
MboII GAAGA 6 cut(s) 118, 133, 187, 235, 253, 560
MhlI GDGCHC 3 cut(s) 87, 153, 349
MnlI CCTC 4 cut(s) 116, 191, 235, 328
MspI CCGG 4 cut(s) 49, 190, 207, 343
MspR9I CCNGG 2 cut(s) 208, 344
MwoI GCNNNNNNNGC 4 cut(s) 283, 344, 463, 560
NciI CCSGG 2 cut(s) 208, 344
NdeII GATC 3 cut(s) 210, 303, 398
NlaIII CATG 1 cut(s) 409
NlaIV GGNNCC 1 cut(s) 347
NmuCI GTSAC 2 cut(s) 88, 520
PaeR7I CTCGAG 1 cut(s) 182
PciSI GCTCTTC 1 cut(s) 170
PcsI WCGNNNNNNNCGW 3 cut(s) 74, 141, 210
PfeI GAWTC 3 cut(s) 8, 372, 445
PflMI CCANNNNNTGG 1 cut(s) 499
PkrI GCNGC 3 cut(s) 231, 479, 505
PspFI CCCAGC 1 cut(s) 457
PspN4I GGNNCC 1 cut(s) 347
PspOMI GGGCCC 1 cut(s) 345
PspPI GGNCC 2 cut(s) 345, 346
PstNI CAGNNNCTG 1 cut(s) 292
SalI GTCGAC 1 cut(s) 329
SapI GCTCTTC 1 cut(s) 170
SatI GCNGC 3 cut(s) 230, 478, 504
Sau3AI GATC 3 cut(s) 210, 303, 398
Sau96I GGNCC 2 cut(s) 345, 346
ScrFI CCNGG 2 cut(s) 208, 344
SduI GDGCHC 3 cut(s) 87, 153, 349
SetI ASST 8 cut(s) 90, 117, 231, 246, 260, 300, 312, 330
SfaNI GCATC 1 cut(s) 453
Sfr274I CTCGAG 1 cut(s) 182
SgrDI CGTCGACG 1 cut(s) 329
SlaI CTCGAG 1 cut(s) 182
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
SsiI CCGC 3 cut(s) 390, 486, 503
SspMI CTAG 1 cut(s) 574
StyD4I CCNGG 2 cut(s) 206, 342
TaiI ACGT 3 cut(s) 90, 117, 330
TaqI TCGA 5 cut(s) 183, 213, 240, 330, 431
TaqII GACCGA 1 cut(s) 391
TauI GCSGC 1 cut(s) 506
TfiI GAWTC 3 cut(s) 8, 372, 445
TscAI CASTG 1 cut(s) 556
TseFI GTSAC 2 cut(s) 88, 520
TseI GCWGC 2 cut(s) 229, 477
Tsp45I GTSAC 2 cut(s) 88, 520
TspDTI ATGAA 1 cut(s) 21
TspGWI ACGGA 1 cut(s) 487
TspRI CASTG 1 cut(s) 556
Van91I CCANNNNNTGG 1 cut(s) 499
VneI GTGCAC 1 cut(s) 83
XhoI CTCGAG 1 cut(s) 182
XmiI GTMKAC 1 cut(s) 330
XspI CTAG 1 cut(s) 574
ZraI GACGTC 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.