Rw6G022540

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0010105
Physical Location & Seq
Forward (+)
44519332 .. 44521388
2057 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G022540.1

Sequence Viewer

Length: 588 bp
ATGTCTCAAAACAAGGTTTTGGACCAATCTGACGAGTACCCAAAAGAGCATTACGGCGGCCAAACATCCATGTCGTCCTCATCAACCACAAGCGTCCACGTCACCGCCCTGGACGGTCTGGTCAACGTCAACTCCCTCTTCACCATCGCGGTCTTCGTGGGCCTGTCCCTGACCGTACCGGGACAAAAGAGCCTCGAGAACCGGACAGCCTGCGACGCCGGCAATGACGTGGCGAAGAAGCTTCTGGTGTTCGAGGTTGTGTCCTTCAGCTTCTTCCTCTTCTCGTCCCTGGTCGCCCAGGGCCTCAAGCTCGCCATCAACTTGCTTAACAGCAAGGAAGTCGACGAGGCCTTCCGGGCCCACATCAACATCAAGGTGCTGAGGTTCGGGATGTTGGGGTCGGCCTTCGGATCCGTCATGGGTTGCGTCTTTCTCATGTTGTCGATGGTCAATGTGATCCAGATCCGGTTGGGGATGTTGTCGTGTGGGAGCAAATCTGCTGTCCACTCTGTTGCGGCTCTTATCGTTTTGGTCTCTACGGCGCTCTTGGTCTATCTCTCTACTGCTATCTATGCTTTTCTTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

195

Amino Acids

20.89

Weight (kDa)

7.76

Isoelectric Point (pI)

31.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AccI GTMKAC 1 cut(s) 342
AccII CGCG 1 cut(s) 149
AciI CCGC 4 cut(s) 57, 105, 149, 515
AclWI GGATC 4 cut(s) 405, 418, 451, 457
AcoI YGGCCR 1 cut(s) 58
AcuI CTGAAG 1 cut(s) 250
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 2 cut(s) 38, 177
AjiI CACGTC 2 cut(s) 100, 229
AjnI CCWGG 3 cut(s) 108, 288, 297
AluBI AGCT 3 cut(s) 241, 270, 310
AluI AGCT 3 cut(s) 241, 270, 310
Alw26I GTCTC 2 cut(s) 9, 538
AlwI GGATC 4 cut(s) 405, 418, 451, 457
Ama87I CYCGRG 1 cut(s) 194
AoxI GGCC 6 cut(s) 58, 160, 301, 348, 357, 402
ApaI GGGCCC 1 cut(s) 361
ArsI GACNNNNNNTTYG 2 cut(s) 486, 518
AspLEI GCGC 1 cut(s) 544
AspS9I GGNCC 5 cut(s) 22, 160, 301, 357, 358
AsuC2I CCSGG 2 cut(s) 180, 356
AsuHPI GGTGA 2 cut(s) 94, 133
AvaI CYCGRG 1 cut(s) 194
AvaII GGWCC 1 cut(s) 22
BaeGI GKGCMC 1 cut(s) 361
BamHI GGATCC 1 cut(s) 410
BanII GRGCYC 1 cut(s) 361
BbsI GAAGAC 1 cut(s) 145
BbvCI CCTCAGC 1 cut(s) 380
BccI CCATC 3 cut(s) 152, 323, 439
BceAI ACGGC 2 cut(s) 70, 555
BcgI CGANNNNNNTGC 2 cut(s) 322, 356
BciT130I CCWGG 3 cut(s) 110, 290, 299
BcnI CCSGG 2 cut(s) 180, 356
BcoDI GTCTC 2 cut(s) 9, 538
BfaI CTAG 1 cut(s) 586
BfoI RGCGCY 1 cut(s) 545
BglI GCCNNNNNGGC 1 cut(s) 356
BisI GCNGC 2 cut(s) 58, 516
BlsI GCNGC 2 cut(s) 59, 517
Bme1390I CCNGG 5 cut(s) 110, 180, 290, 299, 356
Bme18I GGWCC 1 cut(s) 22
BmeT110I CYCGRG 1 cut(s) 194
BmgBI CACGTC 2 cut(s) 100, 229
BmgT120I GGNCC 5 cut(s) 22, 160, 301, 357, 358
BmiI GGNNCC 2 cut(s) 359, 412
BmrFI CCNGG 5 cut(s) 110, 180, 290, 299, 356
BpiI GAAGAC 1 cut(s) 145
Bpu10I CCTNAGC 1 cut(s) 380
BpuEI CTTGAG 1 cut(s) 290
BpuMI CCSGG 2 cut(s) 180, 356
BsaBI GATNNNNATC 1 cut(s) 461
BsaHI GRCGYC 1 cut(s) 216
BsaI GGTCTC 1 cut(s) 538
BsaJI CCNNGG 4 cut(s) 108, 288, 297, 298
BsaWI WCCGGW 2 cut(s) 201, 465
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bse118I RCCGGY 1 cut(s) 218
Bse3DI GCAATG 1 cut(s) 229
Bse8I GATNNNNATC 1 cut(s) 461
BseBI CCWGG 3 cut(s) 110, 290, 299
BseDI CCNNGG 4 cut(s) 108, 288, 297, 298
BseGI GGATG 3 cut(s) 65, 396, 480
BseJI GATNNNNATC 1 cut(s) 461
BseMI GCAATG 1 cut(s) 229
BseMII CTCAG 1 cut(s) 371
BseSI GKGCMC 1 cut(s) 361
Bsh1236I CGCG 1 cut(s) 149
BshFI GGCC 6 cut(s) 60, 162, 303, 350, 359, 404
BsiHKCI CYCGRG 1 cut(s) 194
BsiSI CCGG 5 cut(s) 179, 202, 219, 355, 466
BslFI GGGAC 3 cut(s) 151, 195, 271
BsmAI GTCTC 2 cut(s) 9, 538
BsmFI GGGAC 3 cut(s) 151, 195, 271
BsnI GGCC 6 cut(s) 60, 162, 303, 350, 359, 404
Bso31I GGTCTC 1 cut(s) 538
BsoBI CYCGRG 1 cut(s) 194
Bsp120I GGGCCC 1 cut(s) 357
Bsp1286I GDGCHC 1 cut(s) 361
Bsp143I GATC 3 cut(s) 410, 456, 462
BspACI CCGC 4 cut(s) 57, 105, 149, 515
BspANI GGCC 6 cut(s) 60, 162, 303, 350, 359, 404
BspCNI CTCAG 1 cut(s) 372
BspFNI CGCG 1 cut(s) 149
BspLI GGNNCC 2 cut(s) 359, 412
BspPI GGATC 4 cut(s) 405, 418, 451, 457
BspTNI GGTCTC 1 cut(s) 538
BsrDI GCAATG 1 cut(s) 229
BsrFI RCCGGY 1 cut(s) 218
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 4 cut(s) 108, 288, 297, 298
BssMI GATC 3 cut(s) 410, 456, 462
BssNI GRCGYC 1 cut(s) 216
Bst2UI CCWGG 3 cut(s) 110, 290, 299
Bst4CI ACNGT 2 cut(s) 116, 175
Bst6I CTCTTC 2 cut(s) 143, 284
BstACI GRCGYC 1 cut(s) 216
BstC8I GCNNGC 3 cut(s) 211, 220, 312
BstDEI CTNAG 1 cut(s) 380
BstF5I GGATG 3 cut(s) 65, 396, 480
BstFNI CGCG 1 cut(s) 149
BstH2I RGCGCY 1 cut(s) 545
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 3 cut(s) 413, 459, 465
BstMAI GTCTC 2 cut(s) 9, 538
BstMBI GATC 3 cut(s) 410, 456, 462
BstMWI GCNNNNNNNGC 4 cut(s) 215, 219, 356, 572
BstNI CCWGG 3 cut(s) 110, 290, 299
BstSCI CCNGG 5 cut(s) 108, 178, 288, 297, 354
BstSLI GKGCMC 1 cut(s) 361
BstUI CGCG 1 cut(s) 149
BstV2I GAAGAC 1 cut(s) 145
BstX2I RGATCY 2 cut(s) 410, 462
BstYI RGATCY 2 cut(s) 410, 462
BsuRI GGCC 6 cut(s) 60, 162, 303, 350, 359, 404
BtgZI GCGATG 1 cut(s) 130
BtrI CACGTC 2 cut(s) 100, 229
BtsCI GGATG 3 cut(s) 65, 396, 480
Cac8I GCNNGC 3 cut(s) 211, 220, 312
CfoI GCGC 1 cut(s) 544
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 5 cut(s) 22, 160, 301, 357, 358
CseI GACGC 3 cut(s) 82, 224, 415
Csp6I GTAC 2 cut(s) 37, 176
CviAII CATG 3 cut(s) 70, 418, 436
CviQI GTAC 2 cut(s) 37, 176
DdeI CTNAG 1 cut(s) 380
DpnI GATC 3 cut(s) 412, 458, 464
DpnII GATC 3 cut(s) 410, 456, 462
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 1 cut(s) 58
Eam1104I CTCTTC 2 cut(s) 143, 284
EarI CTCTTC 2 cut(s) 143, 284
Eco147I AGGCCT 1 cut(s) 350
Eco24I GRGCYC 1 cut(s) 361
Eco31I GGTCTC 1 cut(s) 538
Eco47I GGWCC 1 cut(s) 22
Eco57I CTGAAG 1 cut(s) 250
Eco88I CYCGRG 1 cut(s) 194
EcoO109I RGGNCCY 1 cut(s) 301
EcoRII CCWGG 3 cut(s) 108, 288, 297
EcoT38I GRGCYC 1 cut(s) 361
FaeI CATG 3 cut(s) 73, 421, 439
FaiI YATR 4 cut(s) 71, 419, 437, 573
FaqI GGGAC 3 cut(s) 151, 195, 271
FatI CATG 3 cut(s) 69, 417, 435
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 2 cut(s) 58, 516
FokI GGATG 3 cut(s) 52, 403, 487
FriOI GRGCYC 1 cut(s) 361
Fsp4HI GCNGC 2 cut(s) 58, 516
FspBI CTAG 1 cut(s) 586
GlaI GCGC 1 cut(s) 543
GluI GCNGC 2 cut(s) 58, 516
HaeII RGCGCY 1 cut(s) 545
HaeIII GGCC 6 cut(s) 60, 162, 303, 350, 359, 404
HapII CCGG 5 cut(s) 179, 202, 219, 355, 466
HgaI GACGC 3 cut(s) 82, 224, 415
HhaI GCGC 1 cut(s) 544
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 3 cut(s) 73, 421, 439
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HincII GTYRAC 3 cut(s) 124, 130, 343
HindII GTYRAC 3 cut(s) 124, 130, 343
HindIII AAGCTT 1 cut(s) 239
HpaII CCGG 5 cut(s) 179, 202, 219, 355, 466
HphI GGTGA 2 cut(s) 94, 133
Hpy166II GTNNAC 5 cut(s) 97, 124, 130, 343, 505
Hpy188I TCNGA 2 cut(s) 31, 410
Hpy188III TCNNGA 3 cut(s) 196, 388, 460
Hpy8I GTNNAC 5 cut(s) 97, 124, 130, 343, 505
Hpy99I CGWCG 2 cut(s) 218, 347
HpyAV CCTTC 3 cut(s) 274, 361, 415
HpyCH4III ACNGT 2 cut(s) 116, 175
HpyCH4IV ACGT 3 cut(s) 99, 126, 228
HpyF10VI GCNNNNNNNGC 4 cut(s) 215, 219, 356, 572
HpyF3I CTNAG 1 cut(s) 380
HpySE526I ACGT 3 cut(s) 99, 126, 228
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 3 cut(s) 73, 421, 439
HspAI GCGC 1 cut(s) 542
KroI GCCGGC 1 cut(s) 218
KroNI GCCGGC 1 cut(s) 220
Kzo9I GATC 3 cut(s) 410, 456, 462
LmnI GCTCC 1 cut(s) 489
MaeI CTAG 1 cut(s) 586
MaeII ACGT 3 cut(s) 99, 126, 228
MaeIII GTNAC 1 cut(s) 100
MalI GATC 3 cut(s) 412, 458, 464
MboI GATC 3 cut(s) 410, 456, 462
MboII GAAGA 6 cut(s) 130, 145, 247, 265, 271, 572
MflI RGATCY 2 cut(s) 410, 462
MhlI GDGCHC 1 cut(s) 361
MnlI CCTC 8 cut(s) 88, 146, 203, 247, 287, 314, 340, 375
MroNI GCCGGC 1 cut(s) 218
MseI TTAA 1 cut(s) 327
MslI CAYNNNNRTG 1 cut(s) 374
MspI CCGG 5 cut(s) 179, 202, 219, 355, 466
MspR9I CCNGG 5 cut(s) 110, 180, 290, 299, 356
MvaI CCWGG 3 cut(s) 110, 290, 299
MvnI CGCG 1 cut(s) 149
MwoI GCNNNNNNNGC 4 cut(s) 215, 219, 356, 572
NaeI GCCGGC 1 cut(s) 220
NciI CCSGG 2 cut(s) 180, 356
NdeII GATC 3 cut(s) 410, 456, 462
NgoMIV GCCGGC 1 cut(s) 218
NlaIII CATG 3 cut(s) 73, 421, 439
NlaIV GGNNCC 2 cut(s) 359, 412
NmuCI GTSAC 1 cut(s) 100
PaeR7I CTCGAG 1 cut(s) 194
PasI CCCWGGG 1 cut(s) 298
PceI AGGCCT 1 cut(s) 350
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PdiI GCCGGC 1 cut(s) 220
PkrI GCNGC 2 cut(s) 59, 517
Psp6I CCWGG 3 cut(s) 108, 288, 297
PspGI CCWGG 3 cut(s) 108, 288, 297
PspN4I GGNNCC 2 cut(s) 359, 412
PspOMI GGGCCC 1 cut(s) 357
PspPI GGNCC 5 cut(s) 22, 160, 301, 357, 358
PsuI RGATCY 2 cut(s) 410, 462
RsaI GTAC 2 cut(s) 38, 177
RsaNI GTAC 2 cut(s) 37, 176
RseI CAYNNNNRTG 1 cut(s) 374
SalI GTCGAC 1 cut(s) 341
SaqAI TTAA 1 cut(s) 327
SatI GCNGC 2 cut(s) 58, 516
Sau3AI GATC 3 cut(s) 410, 456, 462
Sau96I GGNCC 5 cut(s) 22, 160, 301, 357, 358
ScrFI CCNGG 5 cut(s) 110, 180, 290, 299, 356
SduI GDGCHC 1 cut(s) 361
SfiI GGCCNNNNNGGCC 1 cut(s) 356
Sfr274I CTCGAG 1 cut(s) 194
SinI GGWCC 1 cut(s) 22
SlaI CTCGAG 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 374
SmlI CTYRAG 2 cut(s) 194, 305
SmoI CTYRAG 2 cut(s) 194, 305
SseBI AGGCCT 1 cut(s) 350
SsiI CCGC 4 cut(s) 57, 105, 149, 515
SspMI CTAG 1 cut(s) 586
StuI AGGCCT 1 cut(s) 350
StyD4I CCNGG 5 cut(s) 108, 178, 288, 297, 354
TaaI ACNGT 2 cut(s) 116, 175
TaiI ACGT 3 cut(s) 102, 129, 231
TaqI TCGA 4 cut(s) 195, 252, 342, 443
TauI GCSGC 2 cut(s) 60, 518
Tru1I TTAA 1 cut(s) 327
Tru9I TTAA 1 cut(s) 327
TseFI GTSAC 1 cut(s) 100
Tsp45I GTSAC 1 cut(s) 100
TspGWI ACGGA 1 cut(s) 403
VpaK11BI GGWCC 1 cut(s) 22
XhoI CTCGAG 1 cut(s) 194
XmiI GTMKAC 1 cut(s) 342
XspI CTAG 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.