Rmu_co8275275.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8275275.1
OG0010105
Physical Location & Seq
Forward (+)
1 .. 645
645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8275275.1_g000001.1.cds

Sequence Viewer

Length: 562 bp
atctgacgagtacccaaaagagcattacggcggccaaacatccatgtcgtcctcatcaaccacaagcgtccacgtcaccgccctggacggtctggtcaacgtcaactccctcttcaccatcgcggtcttcgtgggcctgtccctgaccgtaccgggacaaaagagcctcgagaaccggacagcctgcgacgccggcaatgacgtggcgaagaagcttctggtgttcgaggttgtctccttcagcttcttcctcttctcgtccctggtcgcccagggcctcaagctcgccatcaacttgcttaacagcaaggaagtcgacgaggccttccgggcccacatcaacatcaaggtgctgaggttcgggatgttggggtcagccttcggatccgtcatgggttgcgtctttctcatgttgtcgatggtcaatgtgatccagatccggttggggatgttgtcgtgtgggagcaaatctgctgtccactctgttgcggctcttatcgttttggtctctacggcgctcttggtctatctctctactgctatctatgcttttcttcactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

19.84

Weight (kDa)

7.74

Isoelectric Point (pI)

26.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 93
AccI GTMKAC 1 cut(s) 316
AccII CGCG 1 cut(s) 123
AciI CCGC 4 cut(s) 31, 79, 123, 489
AclWI GGATC 4 cut(s) 379, 392, 425, 431
AcoI YGGCCR 1 cut(s) 32
AcuI CTGAAG 1 cut(s) 224
AcyI GRCGYC 1 cut(s) 190
AfaI GTAC 2 cut(s) 12, 151
AjiI CACGTC 2 cut(s) 74, 203
AjnI CCWGG 3 cut(s) 82, 262, 271
AluBI AGCT 3 cut(s) 215, 244, 284
AluI AGCT 3 cut(s) 215, 244, 284
Alw26I GTCTC 2 cut(s) 239, 512
AlwI GGATC 4 cut(s) 379, 392, 425, 431
Ama87I CYCGRG 1 cut(s) 168
AoxI GGCC 5 cut(s) 32, 134, 275, 322, 331
ApaI GGGCCC 1 cut(s) 335
ArsI GACNNNNNNTTYG 2 cut(s) 460, 492
AspLEI GCGC 1 cut(s) 518
AspS9I GGNCC 4 cut(s) 134, 275, 331, 332
AsuC2I CCSGG 2 cut(s) 154, 330
AsuHPI GGTGA 2 cut(s) 68, 107
AvaI CYCGRG 1 cut(s) 168
BaeGI GKGCMC 1 cut(s) 335
BamHI GGATCC 1 cut(s) 384
BanII GRGCYC 1 cut(s) 335
BbsI GAAGAC 1 cut(s) 119
BbvCI CCTCAGC 1 cut(s) 354
BccI CCATC 3 cut(s) 126, 297, 413
BceAI ACGGC 2 cut(s) 44, 529
BcgI CGANNNNNNTGC 2 cut(s) 296, 330
BciT130I CCWGG 3 cut(s) 84, 264, 273
BcnI CCSGG 2 cut(s) 154, 330
BcoDI GTCTC 2 cut(s) 239, 512
BfaI CTAG 1 cut(s) 560
BfoI RGCGCY 1 cut(s) 519
BglI GCCNNNNNGGC 1 cut(s) 330
BisI GCNGC 2 cut(s) 32, 490
BlsI GCNGC 2 cut(s) 33, 491
Bme1390I CCNGG 5 cut(s) 84, 154, 264, 273, 330
BmeT110I CYCGRG 1 cut(s) 168
BmgBI CACGTC 2 cut(s) 74, 203
BmgT120I GGNCC 4 cut(s) 134, 275, 331, 332
BmiI GGNNCC 2 cut(s) 333, 386
BmrFI CCNGG 5 cut(s) 84, 154, 264, 273, 330
BpiI GAAGAC 1 cut(s) 119
BplI GAGNNNNNCTC 2 cut(s) 219, 251
Bpu10I CCTNAGC 1 cut(s) 354
BpuEI CTTGAG 1 cut(s) 264
BpuMI CCSGG 2 cut(s) 154, 330
BsaBI GATNNNNATC 1 cut(s) 435
BsaHI GRCGYC 1 cut(s) 190
BsaI GGTCTC 1 cut(s) 512
BsaJI CCNNGG 4 cut(s) 82, 262, 271, 272
BsaWI WCCGGW 2 cut(s) 175, 439
BsaXI ACNNNNNCTCC 2 cut(s) 90, 120
Bse118I RCCGGY 1 cut(s) 192
Bse3DI GCAATG 1 cut(s) 203
Bse8I GATNNNNATC 1 cut(s) 435
BseBI CCWGG 3 cut(s) 84, 264, 273
BseDI CCNNGG 4 cut(s) 82, 262, 271, 272
BseGI GGATG 3 cut(s) 39, 370, 454
BseJI GATNNNNATC 1 cut(s) 435
BseMI GCAATG 1 cut(s) 203
BseMII CTCAG 1 cut(s) 345
BseSI GKGCMC 1 cut(s) 335
Bsh1236I CGCG 1 cut(s) 123
BshFI GGCC 5 cut(s) 34, 136, 277, 324, 333
BsiHKCI CYCGRG 1 cut(s) 168
BsiSI CCGG 5 cut(s) 153, 176, 193, 329, 440
BslFI GGGAC 3 cut(s) 125, 169, 245
BsmAI GTCTC 2 cut(s) 239, 512
BsmFI GGGAC 3 cut(s) 125, 169, 245
BsnI GGCC 5 cut(s) 34, 136, 277, 324, 333
Bso31I GGTCTC 1 cut(s) 512
BsoBI CYCGRG 1 cut(s) 168
Bsp120I GGGCCC 1 cut(s) 331
Bsp1286I GDGCHC 1 cut(s) 335
Bsp143I GATC 3 cut(s) 384, 430, 436
BspACI CCGC 4 cut(s) 31, 79, 123, 489
BspANI GGCC 5 cut(s) 34, 136, 277, 324, 333
BspCNI CTCAG 1 cut(s) 346
BspFNI CGCG 1 cut(s) 123
BspLI GGNNCC 2 cut(s) 333, 386
BspPI GGATC 4 cut(s) 379, 392, 425, 431
BspTNI GGTCTC 1 cut(s) 512
BsrDI GCAATG 1 cut(s) 203
BsrFI RCCGGY 1 cut(s) 192
BssAI RCCGGY 1 cut(s) 192
BssECI CCNNGG 4 cut(s) 82, 262, 271, 272
BssMI GATC 3 cut(s) 384, 430, 436
BssNI GRCGYC 1 cut(s) 190
Bst2UI CCWGG 3 cut(s) 84, 264, 273
Bst4CI ACNGT 2 cut(s) 90, 149
Bst6I CTCTTC 2 cut(s) 117, 258
BstACI GRCGYC 1 cut(s) 190
BstC8I GCNNGC 3 cut(s) 185, 194, 286
BstDEI CTNAG 1 cut(s) 354
BstF5I GGATG 3 cut(s) 39, 370, 454
BstFNI CGCG 1 cut(s) 123
BstH2I RGCGCY 1 cut(s) 519
BstHHI GCGC 1 cut(s) 518
BstKTI GATC 3 cut(s) 387, 433, 439
BstMAI GTCTC 2 cut(s) 239, 512
BstMBI GATC 3 cut(s) 384, 430, 436
BstMWI GCNNNNNNNGC 4 cut(s) 189, 193, 330, 546
BstNI CCWGG 3 cut(s) 84, 264, 273
BstSCI CCNGG 5 cut(s) 82, 152, 262, 271, 328
BstSLI GKGCMC 1 cut(s) 335
BstUI CGCG 1 cut(s) 123
BstV2I GAAGAC 1 cut(s) 119
BstX2I RGATCY 2 cut(s) 384, 436
BstYI RGATCY 2 cut(s) 384, 436
BsuRI GGCC 5 cut(s) 34, 136, 277, 324, 333
BtgZI GCGATG 1 cut(s) 104
BtrI CACGTC 2 cut(s) 74, 203
BtsCI GGATG 3 cut(s) 39, 370, 454
Cac8I GCNNGC 3 cut(s) 185, 194, 286
CfoI GCGC 1 cut(s) 518
Cfr10I RCCGGY 1 cut(s) 192
Cfr13I GGNCC 4 cut(s) 134, 275, 331, 332
CseI GACGC 3 cut(s) 56, 198, 389
Csp6I GTAC 2 cut(s) 11, 150
CviAII CATG 3 cut(s) 44, 392, 410
CviQI GTAC 2 cut(s) 11, 150
DdeI CTNAG 1 cut(s) 354
DpnI GATC 3 cut(s) 386, 432, 438
DpnII GATC 3 cut(s) 384, 430, 436
DrdI GACNNNNNNGTC 1 cut(s) 93
DseDI GACNNNNNNGTC 1 cut(s) 93
EaeI YGGCCR 1 cut(s) 32
Eam1104I CTCTTC 2 cut(s) 117, 258
EarI CTCTTC 2 cut(s) 117, 258
Eco147I AGGCCT 1 cut(s) 324
Eco24I GRGCYC 1 cut(s) 335
Eco31I GGTCTC 1 cut(s) 512
Eco57I CTGAAG 1 cut(s) 224
Eco88I CYCGRG 1 cut(s) 168
EcoO109I RGGNCCY 1 cut(s) 275
EcoRII CCWGG 3 cut(s) 82, 262, 271
EcoT38I GRGCYC 1 cut(s) 335
FaeI CATG 3 cut(s) 47, 395, 413
FaiI YATR 4 cut(s) 45, 393, 411, 547
FaqI GGGAC 3 cut(s) 125, 169, 245
FatI CATG 3 cut(s) 43, 391, 409
FblI GTMKAC 1 cut(s) 316
Fnu4HI GCNGC 2 cut(s) 32, 490
FokI GGATG 3 cut(s) 26, 377, 461
FriOI GRGCYC 1 cut(s) 335
Fsp4HI GCNGC 2 cut(s) 32, 490
FspBI CTAG 1 cut(s) 560
GlaI GCGC 1 cut(s) 517
GluI GCNGC 2 cut(s) 32, 490
HaeII RGCGCY 1 cut(s) 519
HaeIII GGCC 5 cut(s) 34, 136, 277, 324, 333
HapII CCGG 5 cut(s) 153, 176, 193, 329, 440
HgaI GACGC 3 cut(s) 56, 198, 389
HhaI GCGC 1 cut(s) 518
Hin1I GRCGYC 1 cut(s) 190
Hin1II CATG 3 cut(s) 47, 395, 413
Hin6I GCGC 1 cut(s) 516
HinP1I GCGC 1 cut(s) 516
HincII GTYRAC 3 cut(s) 98, 104, 317
HindII GTYRAC 3 cut(s) 98, 104, 317
HindIII AAGCTT 1 cut(s) 213
HpaII CCGG 5 cut(s) 153, 176, 193, 329, 440
HphI GGTGA 2 cut(s) 68, 107
Hpy166II GTNNAC 5 cut(s) 71, 98, 104, 317, 479
Hpy188I TCNGA 2 cut(s) 5, 384
Hpy188III TCNNGA 3 cut(s) 170, 362, 434
Hpy8I GTNNAC 5 cut(s) 71, 98, 104, 317, 479
Hpy99I CGWCG 2 cut(s) 192, 321
HpyAV CCTTC 3 cut(s) 248, 335, 389
HpyCH4III ACNGT 2 cut(s) 90, 149
HpyCH4IV ACGT 3 cut(s) 73, 100, 202
HpyF10VI GCNNNNNNNGC 4 cut(s) 189, 193, 330, 546
HpyF3I CTNAG 1 cut(s) 354
HpySE526I ACGT 3 cut(s) 73, 100, 202
Hsp92I GRCGYC 1 cut(s) 190
Hsp92II CATG 3 cut(s) 47, 395, 413
HspAI GCGC 1 cut(s) 516
KroI GCCGGC 1 cut(s) 192
KroNI GCCGGC 1 cut(s) 194
Kzo9I GATC 3 cut(s) 384, 430, 436
LmnI GCTCC 1 cut(s) 463
MaeI CTAG 1 cut(s) 560
MaeII ACGT 3 cut(s) 73, 100, 202
MaeIII GTNAC 1 cut(s) 74
MalI GATC 3 cut(s) 386, 432, 438
MboI GATC 3 cut(s) 384, 430, 436
MboII GAAGA 6 cut(s) 104, 119, 221, 239, 245, 546
MflI RGATCY 2 cut(s) 384, 436
MhlI GDGCHC 1 cut(s) 335
MnlI CCTC 8 cut(s) 62, 120, 177, 221, 261, 288, 314, 349
MroNI GCCGGC 1 cut(s) 192
MseI TTAA 1 cut(s) 301
MslI CAYNNNNRTG 1 cut(s) 348
MspI CCGG 5 cut(s) 153, 176, 193, 329, 440
MspR9I CCNGG 5 cut(s) 84, 154, 264, 273, 330
MvaI CCWGG 3 cut(s) 84, 264, 273
MvnI CGCG 1 cut(s) 123
MwoI GCNNNNNNNGC 4 cut(s) 189, 193, 330, 546
NaeI GCCGGC 1 cut(s) 194
NciI CCSGG 2 cut(s) 154, 330
NdeII GATC 3 cut(s) 384, 430, 436
NgoMIV GCCGGC 1 cut(s) 192
NlaIII CATG 3 cut(s) 47, 395, 413
NlaIV GGNNCC 2 cut(s) 333, 386
NmuCI GTSAC 1 cut(s) 74
PaeR7I CTCGAG 1 cut(s) 168
PasI CCCWGGG 1 cut(s) 272
PceI AGGCCT 1 cut(s) 324
PcsI WCGNNNNNNNCGW 1 cut(s) 127
PdiI GCCGGC 1 cut(s) 194
PkrI GCNGC 2 cut(s) 33, 491
Psp6I CCWGG 3 cut(s) 82, 262, 271
PspGI CCWGG 3 cut(s) 82, 262, 271
PspN4I GGNNCC 2 cut(s) 333, 386
PspOMI GGGCCC 1 cut(s) 331
PspPI GGNCC 4 cut(s) 134, 275, 331, 332
PsuI RGATCY 2 cut(s) 384, 436
RsaI GTAC 2 cut(s) 12, 151
RsaNI GTAC 2 cut(s) 11, 150
RseI CAYNNNNRTG 1 cut(s) 348
SalI GTCGAC 1 cut(s) 315
SaqAI TTAA 1 cut(s) 301
SatI GCNGC 2 cut(s) 32, 490
Sau3AI GATC 3 cut(s) 384, 430, 436
Sau96I GGNCC 4 cut(s) 134, 275, 331, 332
ScrFI CCNGG 5 cut(s) 84, 154, 264, 273, 330
SduI GDGCHC 1 cut(s) 335
SetI ASST 9 cut(s) 76, 103, 205, 217, 232, 246, 286, 352, 360
SfiI GGCCNNNNNGGCC 1 cut(s) 330
Sfr274I CTCGAG 1 cut(s) 168
SlaI CTCGAG 1 cut(s) 168
SmiMI CAYNNNNRTG 1 cut(s) 348
SmlI CTYRAG 2 cut(s) 168, 279
SmoI CTYRAG 2 cut(s) 168, 279
SseBI AGGCCT 1 cut(s) 324
SsiI CCGC 4 cut(s) 31, 79, 123, 489
SspMI CTAG 1 cut(s) 560
StuI AGGCCT 1 cut(s) 324
StyD4I CCNGG 5 cut(s) 82, 152, 262, 271, 328
TaaI ACNGT 2 cut(s) 90, 149
TaiI ACGT 3 cut(s) 76, 103, 205
TaqI TCGA 4 cut(s) 169, 226, 316, 417
TauI GCSGC 2 cut(s) 34, 492
Tru1I TTAA 1 cut(s) 301
Tru9I TTAA 1 cut(s) 301
TseFI GTSAC 1 cut(s) 74
Tsp45I GTSAC 1 cut(s) 74
TspGWI ACGGA 1 cut(s) 377
XhoI CTCGAG 1 cut(s) 168
XmiI GTMKAC 1 cut(s) 316
XspI CTAG 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.