Rroxscaffold_7G00186280

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010105
Physical Location & Seq
Reverse (-)
25619571 .. 25620320
750 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00186280.1

Sequence Viewer

Length: 447 bp
ATGTCGTCCTCATCAACCACAAGCGTCCACGTCACCGCCCTGGACGGCCTAGTCAACGTCAACTCCCTCTTCACCATCGCAGTCTTCGTGGGCCTGTCCCTGACCGTACCGGGACAAAAGAGCCTCGAGAACCGGACAGCCTGCGACGCCGGCAATGACGTGGCGAAGAAGCTTCTGGTGTTCGAGGTTGTCTCCTTCAGCTTCTTCCTCTTCTCGTCTCTGGTCGCCCAGGGCCTCAAGCTCGCCATCAACTTGCTTAACAGCAAGGATGTCGACGAGGCCTTCCGGCCCACATCAACCTCAAGATCCGGTTGGGGATGTTGTCGTGTGGGAGCAAATCTGCTGTCCACTCTGTTGCGGCTCTTATCAACAATTCGTGCTATTATTTTCTGGGGCAGAAGTACTACAACTTGCAAGAAGGCATTACGCTCAAGGCAGAAAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

15.95

Weight (kDa)

10.05

Isoelectric Point (pI)

34.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 50
AccI GTMKAC 1 cut(s) 273
AciI CCGC 2 cut(s) 36, 358
AclWI GGATC 1 cut(s) 300
AcuI CTGAAG 1 cut(s) 181
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 2 cut(s) 108, 403
AjiI CACGTC 2 cut(s) 31, 160
AjnI CCWGG 2 cut(s) 39, 228
AluBI AGCT 3 cut(s) 172, 201, 241
AluI AGCT 3 cut(s) 172, 201, 241
Alw26I GTCTC 2 cut(s) 196, 222
AlwI GGATC 1 cut(s) 300
Ama87I CYCGRG 1 cut(s) 125
AoxI GGCC 5 cut(s) 46, 91, 232, 279, 287
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
AspS9I GGNCC 3 cut(s) 91, 232, 288
AsuC2I CCSGG 1 cut(s) 111
AsuHPI GGTGA 2 cut(s) 25, 64
AvaI CYCGRG 1 cut(s) 125
BbsI GAAGAC 1 cut(s) 76
BccI CCATC 2 cut(s) 83, 254
BceAI ACGGC 1 cut(s) 61
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BciT130I CCWGG 2 cut(s) 41, 230
BcnI CCSGG 1 cut(s) 111
BcoDI GTCTC 2 cut(s) 196, 222
BfaI CTAG 2 cut(s) 50, 445
BisI GCNGC 1 cut(s) 359
BlsI GCNGC 1 cut(s) 360
BmcAI AGTACT 1 cut(s) 403
Bme1390I CCNGG 3 cut(s) 41, 111, 230
BmeT110I CYCGRG 1 cut(s) 125
BmgBI CACGTC 2 cut(s) 31, 160
BmgT120I GGNCC 3 cut(s) 91, 232, 288
BmrFI CCNGG 3 cut(s) 41, 111, 230
BpiI GAAGAC 1 cut(s) 76
BplI GAGNNNNNCTC 2 cut(s) 176, 208
BpuEI CTTGAG 3 cut(s) 221, 286, 415
BpuMI CCSGG 1 cut(s) 111
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 3 cut(s) 39, 228, 229
BsaWI WCCGGW 2 cut(s) 132, 308
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bse118I RCCGGY 1 cut(s) 149
Bse3DI GCAATG 1 cut(s) 160
BseBI CCWGG 2 cut(s) 41, 230
BseDI CCNNGG 3 cut(s) 39, 228, 229
BseGI GGATG 2 cut(s) 274, 323
BseMI GCAATG 1 cut(s) 160
BshFI GGCC 5 cut(s) 48, 93, 234, 281, 289
BsiHKCI CYCGRG 1 cut(s) 125
BsiSI CCGG 5 cut(s) 110, 133, 150, 286, 309
BslFI GGGAC 2 cut(s) 82, 126
BsmAI GTCTC 2 cut(s) 196, 222
BsmBI CGTCTC 1 cut(s) 222
BsmFI GGGAC 2 cut(s) 82, 126
BsnI GGCC 5 cut(s) 48, 93, 234, 281, 289
BsoBI CYCGRG 1 cut(s) 125
Bsp143I GATC 1 cut(s) 305
BspACI CCGC 2 cut(s) 36, 358
BspANI GGCC 5 cut(s) 48, 93, 234, 281, 289
BspPI GGATC 1 cut(s) 300
BsrDI GCAATG 1 cut(s) 160
BsrFI RCCGGY 1 cut(s) 149
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 3 cut(s) 39, 228, 229
BssMI GATC 1 cut(s) 305
BssNI GRCGYC 1 cut(s) 147
Bst2UI CCWGG 2 cut(s) 41, 230
Bst4CI ACNGT 1 cut(s) 106
Bst6I CTCTTC 2 cut(s) 74, 215
BstACI GRCGYC 1 cut(s) 147
BstC8I GCNNGC 3 cut(s) 142, 151, 243
BstF5I GGATG 2 cut(s) 274, 323
BstKTI GATC 1 cut(s) 308
BstMAI GTCTC 2 cut(s) 196, 222
BstMBI GATC 1 cut(s) 305
BstMWI GCNNNNNNNGC 2 cut(s) 146, 150
BstNI CCWGG 2 cut(s) 41, 230
BstSCI CCNGG 3 cut(s) 39, 109, 228
BstV2I GAAGAC 1 cut(s) 76
BstX2I RGATCY 1 cut(s) 305
BstYI RGATCY 1 cut(s) 305
BsuRI GGCC 5 cut(s) 48, 93, 234, 281, 289
BtgZI GCGATG 1 cut(s) 61
BtrI CACGTC 2 cut(s) 31, 160
BtsCI GGATG 2 cut(s) 274, 323
Cac8I GCNNGC 3 cut(s) 142, 151, 243
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 3 cut(s) 91, 232, 288
CseI GACGC 2 cut(s) 13, 155
Csp6I GTAC 2 cut(s) 107, 402
CviQI GTAC 2 cut(s) 107, 402
DpnI GATC 1 cut(s) 307
DpnII GATC 1 cut(s) 305
DrdI GACNNNNNNGTC 1 cut(s) 50
DseDI GACNNNNNNGTC 1 cut(s) 50
Eam1104I CTCTTC 2 cut(s) 74, 215
EarI CTCTTC 2 cut(s) 74, 215
Eco147I AGGCCT 1 cut(s) 281
Eco57I CTGAAG 1 cut(s) 181
Eco88I CYCGRG 1 cut(s) 125
EcoO109I RGGNCCY 1 cut(s) 232
EcoRII CCWGG 2 cut(s) 39, 228
Esp3I CGTCTC 1 cut(s) 222
FaqI GGGAC 2 cut(s) 82, 126
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 1 cut(s) 359
FokI GGATG 2 cut(s) 281, 330
Fsp4HI GCNGC 1 cut(s) 359
FspBI CTAG 2 cut(s) 50, 445
GluI GCNGC 1 cut(s) 359
HaeIII GGCC 5 cut(s) 48, 93, 234, 281, 289
HapII CCGG 5 cut(s) 110, 133, 150, 286, 309
HgaI GACGC 2 cut(s) 13, 155
Hin1I GRCGYC 1 cut(s) 147
HincII GTYRAC 3 cut(s) 55, 61, 274
HindII GTYRAC 3 cut(s) 55, 61, 274
HindIII AAGCTT 1 cut(s) 170
HpaII CCGG 5 cut(s) 110, 133, 150, 286, 309
HphI GGTGA 2 cut(s) 25, 64
Hpy166II GTNNAC 5 cut(s) 28, 55, 61, 274, 348
Hpy188III TCNNGA 2 cut(s) 127, 303
Hpy8I GTNNAC 5 cut(s) 28, 55, 61, 274, 348
Hpy99I CGWCG 2 cut(s) 149, 278
HpyAV CCTTC 3 cut(s) 205, 292, 412
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4IV ACGT 3 cut(s) 30, 57, 159
HpyCH4V TGCA 1 cut(s) 414
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 150
HpySE526I ACGT 3 cut(s) 30, 57, 159
Hsp92I GRCGYC 1 cut(s) 147
KroI GCCGGC 1 cut(s) 149
KroNI GCCGGC 1 cut(s) 151
Kzo9I GATC 1 cut(s) 305
LmnI GCTCC 1 cut(s) 332
MaeI CTAG 2 cut(s) 50, 445
MaeII ACGT 3 cut(s) 30, 57, 159
MaeIII GTNAC 1 cut(s) 31
MalI GATC 1 cut(s) 307
MboI GATC 1 cut(s) 305
MboII GAAGA 5 cut(s) 61, 76, 178, 196, 202
MflI RGATCY 1 cut(s) 305
MluCI AATT 1 cut(s) 372
MnlI CCTC 8 cut(s) 19, 77, 134, 178, 218, 245, 271, 310
MroNI GCCGGC 1 cut(s) 149
MseI TTAA 1 cut(s) 258
MspI CCGG 5 cut(s) 110, 133, 150, 286, 309
MspR9I CCNGG 3 cut(s) 41, 111, 230
MvaI CCWGG 2 cut(s) 41, 230
MwoI GCNNNNNNNGC 2 cut(s) 146, 150
NaeI GCCGGC 1 cut(s) 151
NciI CCSGG 1 cut(s) 111
NdeII GATC 1 cut(s) 305
NgoMIV GCCGGC 1 cut(s) 149
NmuCI GTSAC 1 cut(s) 31
PaeR7I CTCGAG 1 cut(s) 125
PasI CCCWGGG 1 cut(s) 229
PceI AGGCCT 1 cut(s) 281
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PdiI GCCGGC 1 cut(s) 151
PkrI GCNGC 1 cut(s) 360
Psp6I CCWGG 2 cut(s) 39, 228
PspGI CCWGG 2 cut(s) 39, 228
PspPI GGNCC 3 cut(s) 91, 232, 288
PsuI RGATCY 1 cut(s) 305
RsaI GTAC 2 cut(s) 108, 403
RsaNI GTAC 2 cut(s) 107, 402
SalI GTCGAC 1 cut(s) 272
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 1 cut(s) 359
Sau3AI GATC 1 cut(s) 305
Sau96I GGNCC 3 cut(s) 91, 232, 288
ScaI AGTACT 1 cut(s) 403
ScrFI CCNGG 3 cut(s) 41, 111, 230
SetI ASST 9 cut(s) 33, 60, 162, 174, 189, 203, 243, 302, 446
Sfr274I CTCGAG 1 cut(s) 125
SlaI CTCGAG 1 cut(s) 125
SmlI CTYRAG 4 cut(s) 125, 236, 301, 430
SmoI CTYRAG 4 cut(s) 125, 236, 301, 430
Sse9I AATT 1 cut(s) 372
SseBI AGGCCT 1 cut(s) 281
SsiI CCGC 2 cut(s) 36, 358
SspMI CTAG 2 cut(s) 50, 445
StuI AGGCCT 1 cut(s) 281
StyD4I CCNGG 3 cut(s) 39, 109, 228
TaaI ACNGT 1 cut(s) 106
TaiI ACGT 3 cut(s) 33, 60, 162
TaqI TCGA 3 cut(s) 126, 183, 273
TasI AATT 1 cut(s) 372
TatI WGTACW 1 cut(s) 401
TauI GCSGC 1 cut(s) 361
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TseFI GTSAC 1 cut(s) 31
Tsp45I GTSAC 1 cut(s) 31
XhoI CTCGAG 1 cut(s) 125
XmiI GTMKAC 1 cut(s) 273
XspI CTAG 2 cut(s) 50, 445
ZrmI AGTACT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.