pycom10g17130

Snapin/Pallidin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009347
Physical Location & Seq
Reverse (-)
20310581 .. 20311102
522 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 429 bp
ATGGAAAGCCAGAAAACCGAGCTGGAGTCATCAGAGGAGACGAGTGTGACTGTGAACCAGAACGAGAACGCGAAGAACAGATTACAGTCGACGGAGGCAAAGGCGCTTTCGTCGATGCTCACCACCGTCATCAGAGACTTCGATTACAAAGCTCAGCAGACCTTCGCCAGCCAGGACCACCTCTCTCAGTCGCTCCATCGCCTCACCCGCGTCTTGTGGGCAGAGCTGGATAAGTTGTTGGACGCTGCACCGTCTCCGTTCATCATGCAACACGCCGCCAAGAATTCCGCCGTTAGGAAGAGAGTTTCGTCGTTGAATTCGGTTCTCAAGTCCGTACAGCGCCGGCTTGATAACATCGACAGAATGCTGTTTGTCGGTGTTCCAAATCAGCATCAGCAGCAACAGCAACATGGTATTTGCAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Snapin_Pallidin

Protein Analysis

143

Amino Acids

16.16

Weight (kDa)

9.41

Isoelectric Point (pI)

57.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Snapin_Pallidin PF14712 33 - 119 8.9e-15 Snapin/Pallidin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 89
AccII CGCG 2 cut(s) 71, 210
AciI CCGC 3 cut(s) 208, 276, 288
AcsI RAATTY 2 cut(s) 283, 316
AfaI GTAC 1 cut(s) 336
AfiI CCNNNNNNNGG 1 cut(s) 294
AgsI TTSAA 1 cut(s) 316
AjnI CCWGG 1 cut(s) 171
AluBI AGCT 3 cut(s) 22, 152, 226
AluI AGCT 3 cut(s) 22, 152, 226
Alw26I GTCTC 3 cut(s) 32, 129, 258
ApeKI GCWGC 2 cut(s) 245, 397
ApoI RAATTY 2 cut(s) 283, 316
AspLEI GCGC 2 cut(s) 106, 342
AspS9I GGNCC 1 cut(s) 175
AsuHPI GGTGA 2 cut(s) 112, 196
AvaII GGWCC 1 cut(s) 175
BbvI GCAGC 2 cut(s) 232, 409
BccI CCATC 1 cut(s) 204
BceAI ACGGC 1 cut(s) 275
BciT130I CCWGG 1 cut(s) 173
BcoDI GTCTC 3 cut(s) 32, 129, 258
BfoI RGCGCY 2 cut(s) 107, 343
BisI GCNGC 3 cut(s) 246, 276, 398
BlpI GCTNAGC 1 cut(s) 153
BlsI GCNGC 3 cut(s) 247, 277, 399
Bme1390I CCNGG 1 cut(s) 173
Bme18I GGWCC 1 cut(s) 175
BmgT120I GGNCC 1 cut(s) 175
BmrFI CCNGG 1 cut(s) 173
BmsI GCATC 2 cut(s) 105, 400
BpmI CTGGAG 1 cut(s) 44
Bpu1102I GCTNAGC 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 311
Bsc4I CCNNNNNNNGG 1 cut(s) 294
Bse118I RCCGGY 1 cut(s) 342
BseBI CCWGG 1 cut(s) 173
BseLI CCNNNNNNNGG 1 cut(s) 294
BseMII CTCAG 2 cut(s) 167, 200
BseRI GAGGAG 1 cut(s) 50
BseXI GCAGC 2 cut(s) 232, 409
BsgI GTGCAG 1 cut(s) 231
Bsh1236I CGCG 2 cut(s) 71, 210
BsiSI CCGG 1 cut(s) 343
BslI CCNNNNNNNGG 1 cut(s) 294
BsmAI GTCTC 3 cut(s) 32, 129, 258
BsmBI CGTCTC 2 cut(s) 32, 258
BsmI GAATGC 1 cut(s) 369
Bsp1720I GCTNAGC 1 cut(s) 153
BspACI CCGC 3 cut(s) 208, 276, 288
BspCNI CTCAG 2 cut(s) 166, 199
BspFNI CGCG 2 cut(s) 71, 210
BsrFI RCCGGY 1 cut(s) 342
BssAI RCCGGY 1 cut(s) 342
Bst2UI CCWGG 1 cut(s) 173
Bst4CI ACNGT 4 cut(s) 52, 87, 127, 252
Bst6I CTCTTC 1 cut(s) 293
BstC8I GCNNGC 2 cut(s) 169, 344
BstDEI CTNAG 2 cut(s) 153, 186
BstFNI CGCG 2 cut(s) 71, 210
BstH2I RGCGCY 2 cut(s) 107, 343
BstHHI GCGC 2 cut(s) 106, 342
BstMAI GTCTC 3 cut(s) 32, 129, 258
BstMWI GCNNNNNNNGC 3 cut(s) 207, 397, 403
BstNI CCWGG 1 cut(s) 173
BstSCI CCNGG 1 cut(s) 171
BstUI CGCG 2 cut(s) 71, 210
BstV1I GCAGC 2 cut(s) 232, 409
BtgZI GCGATG 1 cut(s) 182
Cac8I GCNNGC 2 cut(s) 169, 344
CfoI GCGC 2 cut(s) 106, 342
Cfr10I RCCGGY 1 cut(s) 342
Cfr13I GGNCC 1 cut(s) 175
CseI GACGC 2 cut(s) 199, 251
Csp6I GTAC 1 cut(s) 335
CviAII CATG 2 cut(s) 265, 410
CviJI RGCY 6 cut(s) 9, 22, 152, 171, 226, 346
CviKI_1 RGCY 6 cut(s) 9, 22, 152, 171, 226, 346
CviQI GTAC 1 cut(s) 335
DdeI CTNAG 2 cut(s) 153, 186
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
EciI GGCGGA 1 cut(s) 277
Eco47I GGWCC 1 cut(s) 175
EcoRI GAATTC 2 cut(s) 283, 316
EcoRII CCWGG 1 cut(s) 171
Esp3I CGTCTC 2 cut(s) 32, 258
FaeI CATG 2 cut(s) 268, 413
FaiI YATR 2 cut(s) 266, 411
FatI CATG 2 cut(s) 264, 409
FauI CCCGC 1 cut(s) 215
FblI GTMKAC 1 cut(s) 89
Fnu4HI GCNGC 3 cut(s) 246, 276, 398
Fsp4HI GCNGC 3 cut(s) 246, 276, 398
GlaI GCGC 2 cut(s) 105, 341
GluI GCNGC 3 cut(s) 246, 276, 398
GsuI CTGGAG 1 cut(s) 44
HaeII RGCGCY 2 cut(s) 107, 343
HapII CCGG 1 cut(s) 343
HgaI GACGC 2 cut(s) 199, 251
HhaI GCGC 2 cut(s) 106, 342
Hin1II CATG 2 cut(s) 268, 413
Hin6I GCGC 2 cut(s) 104, 340
HinP1I GCGC 2 cut(s) 104, 340
HincII GTYRAC 1 cut(s) 90
HindII GTYRAC 1 cut(s) 90
HinfI GANTC 1 cut(s) 26
HpaII CCGG 1 cut(s) 343
HphI GGTGA 2 cut(s) 112, 196
Hpy166II GTNNAC 2 cut(s) 55, 90
Hpy188I TCNGA 2 cut(s) 34, 134
Hpy8I GTNNAC 2 cut(s) 55, 90
Hpy99I CGWCG 3 cut(s) 94, 115, 313
HpyAV CCTTC 1 cut(s) 172
HpyCH4III ACNGT 4 cut(s) 52, 87, 127, 252
HpyCH4V TGCA 3 cut(s) 248, 268, 420
HpyF10VI GCNNNNNNNGC 3 cut(s) 207, 397, 403
HpyF3I CTNAG 2 cut(s) 153, 186
Hsp92II CATG 2 cut(s) 268, 413
HspAI GCGC 2 cut(s) 104, 340
KroI GCCGGC 1 cut(s) 342
KroNI GCCGGC 1 cut(s) 344
LmnI GCTCC 1 cut(s) 198
LpnPI CCDG 8 cut(s) 8, 23, 71, 158, 181, 185, 212, 356
Lsp1109I GCAGC 2 cut(s) 232, 409
LweI GCATC 2 cut(s) 105, 400
MaeIII GTNAC 1 cut(s) 46
MboII GAAGA 2 cut(s) 85, 310
MluCI AATT 2 cut(s) 283, 316
MlyI GAGTC 1 cut(s) 35
MmeI TCCRAC 1 cut(s) 219
MnlI CCTC 4 cut(s) 28, 88, 191, 212
MroNI GCCGGC 1 cut(s) 342
MspI CCGG 1 cut(s) 343
MspR9I CCNGG 1 cut(s) 173
Mva1269I GAATGC 1 cut(s) 369
MvaI CCWGG 1 cut(s) 173
MvnI CGCG 2 cut(s) 71, 210
MwoI GCNNNNNNNGC 3 cut(s) 207, 397, 403
NaeI GCCGGC 1 cut(s) 344
NgoMIV GCCGGC 1 cut(s) 342
NlaIII CATG 2 cut(s) 268, 413
NmuCI GTSAC 1 cut(s) 46
PctI GAATGC 1 cut(s) 369
PdiI GCCGGC 1 cut(s) 344
PkrI GCNGC 3 cut(s) 247, 277, 399
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
Psp6I CCWGG 1 cut(s) 171
PspGI CCWGG 1 cut(s) 171
PspPI GGNCC 1 cut(s) 175
RsaI GTAC 1 cut(s) 336
RsaNI GTAC 1 cut(s) 335
SalI GTCGAC 1 cut(s) 88
SatI GCNGC 3 cut(s) 246, 276, 398
Sau96I GGNCC 1 cut(s) 175
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 1 cut(s) 173
SetI ASST 5 cut(s) 24, 154, 164, 183, 228
SfaNI GCATC 2 cut(s) 105, 400
SinI GGWCC 1 cut(s) 175
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
Sse9I AATT 2 cut(s) 283, 316
SsiI CCGC 3 cut(s) 208, 276, 288
StyD4I CCNGG 1 cut(s) 171
TaaI ACNGT 4 cut(s) 52, 87, 127, 252
TaqI TCGA 4 cut(s) 89, 113, 141, 357
TasI AATT 2 cut(s) 283, 316
TauI GCSGC 1 cut(s) 278
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 2 cut(s) 245, 397
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 1 cut(s) 250
TspGWI ACGGA 3 cut(s) 107, 246, 322
VpaK11BI GGWCC 1 cut(s) 175
XapI RAATTY 2 cut(s) 283, 316
XmiI GTMKAC 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.