pycom10g24170

HB1, ASXL, restriction endonuclease HTH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011708
Physical Location & Seq
Forward (+)
26060484 .. 26070068
9585 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5361 bp
ATGGAGGCGGCATCGGAGGGAGAAAATCAGAACAAAAATCATGAGAGCAATAGTAAATTTAACAATTCCAGTGAGGGGCAGAGCAAGCCCAAGCGTCAGATGAAGACCCCCTTTCAGCTCGAAACCCTCGAGAAAGCATATGCCTTGGATACCTACCCGTCGGAGGCGGTTAGGGCTGAGCTGTCGGAAAGATTAGGGCTTTCCGATCGGCAGTTGCAGATGTGGTTCTGTCACAGAAGGTTGAAGGACAAGAAGGAAGGGGGTCCACCCAAGAAGCAGCGGAAATTGGCACCCGCTTTGCCTGAGCCTCCTATGGATGATTTGGCTCATGGCTCAGAGCCGGGTAGTGATTATGGGTCGGGCTCCGGCTCCGGCTCAAGCCCCTTTGGTCATGCACAGTTGCGGAATGTGGTGGCTAGGGGTGTGGCTGATGACATTCCAATGAGGGGGAGGAGGTATTATGAGTCGCCACAGTCGAAATTGGAGCTCAGAGCCATTGCTTGTGTGGAAGCTCAATTGGGAGAGCCATTGAGGGAAAATGGCCCGCTTCTTGGGATAGAGTTTGATCCTTTGCCGCCAGATGCATTTGGGGCACCTATAGTTGCGGAGCAGCTGAAGCGGTCTGCCCATGCTTTGGAGGGCAAATATGAGCGTCATGATGCAAAACCAAATAAAGTTGCTCCAAGGACCCTCCATGAATATCCCTTTCCTCAAGATCACTCCAGTATTCGGTCTGATGCATATGGACAAGTCTCTCAATCTCATTTTCACGATTCAGCCATTGATGGTCCATCTGCAAGAACTTCATCATTTGCTGTTGGAAATGAGCCGTTGTCAAGGGTTCATGTTGTCCATGGTCATGCGTCGCGTGTTCGTCTTCTGTCTCAGCAGGAGAGGCAAGCGGTGCCCTACTCATCTCCTGTTGATGATGGAAGTCTACCACAAAGGGACTCCTTCACTAATGTAAGAGTAAATACTCAGTTTAGTGATCCCCCTGTTGTTGCACCGGAAAACTCTAATGTATTATCTGAAGATCAAATTAATGACTCTATTCTGCGAATGGAGAGGAAACGCAAGAGTGAAGAAGCAAGAATCGCTAAAGAAGTTGAAGCTCATGAGCTGCGGACCCGGAAGGAACTGGAGAAACAAGATATTCTGAGGAGAAAGAACGAGGAACGAATGAGGAAGGAAATGGAAAGGCAAGACCGTGAAAGACGAAAAGAAGAAGAGAGGTTGATGCGTGAAAGGCAGCGCGAGGAAGAGAGATTAAAGCGTGAGCAAAAACGTGAAATTGAACGAAGGGAAAAGTTTTTACAGAAAGAGTATATAAGAGCAGAGAAAAGGAGGCAGAAGGAAGAGCTTCGCAAAGAGAGAGAGGTAGTGAGACGCAAAGCTGCACTTGAGAAGGCAACTGCACGCAGGCTTGCTAAAGAATCTATGGAGCTTATTGAGGATGAGCAACTAGAACTAATGGAGTTGGCAGCTGCAAGCAAGGGTTTGTCCTCCATTATTTCTATTGATCTTGACACCCTGCAAAACCTTGATGCATTCAGAGATTCTTTGGTTGCATTCCCACCTACGTCTGTGCAATTAAAAAGACCATTTGCAATTCAGCCGTGGATCAATTCAGAGGCAAATATTGGTAACCTTCTCATGGTTTGGAGATTTTTGATTACCTTCGCTGATGTTCTCGAGTTATGGCCCTTTACTCTTGATGAGTTTATTCAAGCATTTCATGACTATGATTCAAGGTTGTTGGGTGAGATCCATCTTGCTCTCTTGAGGTTGATTATCAAAGATATTGAAGATGTTGCAAGGACTCCTACTGGATTAGGAATAAATCAAAATGGTGCTGCTAATCCTGGAGGTGGACATCCACAGATTGTTGAAGGAGCATATGCATGGGGCTTTGATATACGAAACTGGCAGCAGCACTTAAATCTGCTAACATGGCCTGAAATATTCCGGCAACTAGCACTCTCTGCCGGATTTGGGCCACAGCTGAAGAAAAGGAGTATTGCATGGTCATACACACCTGATAAGGATGAGGGTAAAGTTTGTCAGGATGCCATTTCTAATCTACGAAATGGTTCTGCAGCTGTAAATGCATTTGCAGTAATGCAAGAGAAAGGCTTACTGGCTCCAAGAAGATCTAGACACCGGTTGACTCCTGGAACTGTAAAGTTTGCAGCTTTTCATGTGCTTTCACTTGAGGGAAATAAGGGACTAACAGTGTTAGATCTTGCAGAAAAGATTCAGAAATCTGGCCTTCGGGACCTGACAACAAGCAAGACTCCCGAAGCTTCAATTTCTGTTGCTTTGACAAGAGATACTAAGCTCTTTGAAAGAATAGCTCCTTCAACATATCGTGTACGAGCTGCTTACAGAAAGATCGATCCTATTGATGCTGAGGCTGTACTTTCAGCGGCCAGGAAGAAAATTCAGATATTTGAAAATGGAATTTTAGCTGCGGAAGATGTCGATGATGTTGAAAGAGATGATGTTGAAAATGATGAGGTTGAAAGAGACGAAGACTTTGAATGTGAAGTTGATGAAGATCATGATGTTGATGACTTAGCTACTCCATCAGTTGCAAAGAAATCCCCTGATGATTACAGTGAAGTAATTAGTTTCTCAGAAAATGGAAAGGACAGTTTATGCGGCGATGTTGGACTTGCTGTGAAAAATGAGTTTGATAAGGATGTTTCATGTTCCCCCGTCACTGGTTCCAAAGATGCATTCTGTCCTAGTGCTTCCTCAAAACAGTGTGTTTCAGGTGTAGATATTAGTAATAGTAATCTTGACCAAGAAAATATGGAGATTGATGAAAGCAAGTCAGGTGAGTCATGGGTTCAAGGACTTACAGAAGGGGAATATTCTGATCTCAGCGTGGAAGAGCGTCTTAGAGGTCTTGTTGCCTTGATTGGTGTTGCAAATGAAGGAAACACTATTCGTGTTGTTCTTGAGGATCGATTAGAAGCTGCAAATGCTCTTAAGAAGCAAATGTTGGCAGAGGCACAGCTGGATAAAAGTCGCCTAAAAGAAGAGAATGTTGGTAGGTTAGATTTTCAATCTTTTATGGGAGGCAAAGCTGACGTACAAGCTACCGGAGTAGAGGACGGCCAAAGTCCCTTACGGGATGTTGATAACAGAAACATTGAAGCATCTCCAGTCACTGCAGAAAATCAAAAGTCTATCCATGGGTCACAAGGTGTTCAGAATCAGCTAAATGGTGTGCCTGTTGAAAGGACCTCAGCAGCCCAAGATTTCTCCATGGGTTCTGATAACTTTTTGTCTCAGCAACTTGCATATGCATCAAAAAGGTCACGCTCACAGTTAAAATCATATATTGCACATAGAGCAGAAGAGATGTATGCAGACAGGTCCCTGCCTCTTGGTCAAGATCGTAGGCATAATCGATACTGGCAATTTGTTGCATCTGCATCAAGAAATGATCCTGGTTCAGGCAGAATTTTTATTGAATTGAACAATGGAAGCTGGAGGCTTATTGACACTGAAGGGGCCTTTGATGCTCTTGTTACGGCTCTGGACACACGCGGGATTAGGGAATCTCATTTACGCTTAATGTTGCAAAAGATTGAGGCATCCTTCAAGGAGAATGGTCGGAAAAATGCACATTGTCCTAACAATGCAGGCCCGAGTAAAAACCATGTTAAAAATGAAGCCGGCGAAATGGATTCTAGTCCTGATTTCCCTTCTCGTTTTGACAGCCCTGAAAGTACAGTCTGTGCTTTGAATTCTGATACAACGCAGAGCTCCTCTTCTTTCAGAATTGAGCTCGACAGAAGTGAAACTGAGAAAAGGGCTGCCTTGAGAAGGTATCAAGATTTTCAGAAGTGGATGTGGAAAGAATGCTTTAATTCATCTACCTTAGGTGCCACGAAATATGGGAAGGAGAGATGCAGACTGCTGTTTGATGTTTGTGATGTCTGCCTCAGTTGTTATTACTTTGAGGATTCCCACTGCGATACTTGCCATCAGACTTTTGATGCTTTCAACAGAAATTTTGACTTTAGTGAACATGTAATTCAATGTAAAAGAAAAAGGACGTTGGAACCTTGGGATTTCCATGTTCCTTGTACTTCTGTTCCCTTGGCCAGGAGATTGCTCAAGGCATTAATAGCTCTTGTTGAGGTATCTACCCCACCAGAGGCTCTTCAATCGGTTTGGACTGAAGACCGTCGAAAGATGTGGGGAGAGAAACTGAATGCGTCATCATCAATGGAAGAACTTCTACAGACATTGACTCTGTTTGAGACTGCTGTGAAGAGAGACTTTCTGTCATCACACTTTGCACTGACAGAGGAATTACTGGGCTCCTGCAAACAGTCGGGGGTTGTTCGCGATTATCTTGACAGTGGATCCGTTCCTGTACTTCCTTGGATACCACTTACCACTGCAGCTGTGGCTCTGAGGCTTCATGAGATAGATTCAGCAATTACATATGTAAAGCAAGCGAAACCTGAGCCCACTGCGGACAAGGAAATGGGAGAATATCTTAATCAACCTATGAGACTTCTTACATCGAGGAGTGAGACTGAACTAACAGAAGCAGGCCTCAATGAGTACACGAGGGAAGACAACAGTACTCACCTGAAAAGTGGACGAAACAGCTTTAGACGTGGTAGAGGAGGTCGAGAGCAAGGACGCGGTAAAAGGTGGCAGAAAAAGGTGAATAATCCCAAATCAGTGGTAAGTCGTCGGAACATTAGGGGTAATGAGAATTTGAGTCAAGGGTTGAGAACTGTGGGAAAACGAACGCAGGGACAAGGGAGTGGACGGGGTCGCCGAACTGTTAGAAAGAGGAGAATGAAGAACAGGGCTGTTGAGGAGACACCTCTGGGCCGTATGAGTGACATACGCAGCAGCCCTGAGAGTGGAGGAGAATCGCCAAGAAACTTGGCTGAGGAATGGGATGATGAAAAGGTCGATATGAAAGGTGACGAGCAAGGAGAGGGATATGAACAAGGAGAGTTGGAAGAGGGATATGAACAAGGAGAGTTAGGAGAGGGATATGAACAAGGAGAGTTAGGAGAGGGATATGAACAAGGAGGCGCATTGGAATCTGAAGACGACGAACAAGCAGTGGGATATGAACAAGGTAACTGGGAAATTGGGTTTGATGGGACTACCAGCGGATGGAACAGGGGTTTAGTAGAAGCTAGTGACAACGATTTGGATACTTCTGAAGATGACAATAACGTCATGGAAGAAGTGAGAGATGAAGATTCAGAAGGGGATGTAGATGTCGATATGAGTGATGCCTCAGATGAAATGCCAAACGCGATGAGAAACGATGATGGCACAGAGTCGGAAACTTCAGATGACTATAGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000122 GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000982 GO:0000987 GO:0001012 GO:0001067 GO:0001077 GO:0001227 GO:0001228 GO:0001525 GO:0001568 GO:0001944 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009791 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030154 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0035239 GO:0035295 GO:0040034 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048506 GO:0048509 GO:0048510 GO:0048514 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0072358 GO:0072359 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1990837 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT HARE-HTH Homeobox WHIM1 WSD

Protein Analysis

1787

Amino Acids

201.0

Weight (kDa)

5.25

Isoelectric Point (pI)

52.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 29 - 85 1e-16 Homeodomain
DDT PF02791 546 - 600 1.2e-19 DDT domain
HARE-HTH PF05066 725 - 793 2e-18 HB1, ASXL, restriction endonuclease HTH domain
WHIM1 PF15612 945 - 989 7.3e-07 WSTF, HB1, Itc1p, MBD9 motif 1
WSD PF15613 1128 - 1200 1.9e-20 Williams-Beuren syndrome DDT (WSD), D-TOX E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011708)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28420
fragaria_vesca FvH4_3g08620
malus_domestica MD05G1310200.v1.1 MD10G1289100.v1.1
prunus_persica Prupe.4G052600_v2.0.a1
pyrus_communis pycom05g28660 pycom10g24170
rosa_chinensis RchiOBHm_Chr5g0009221
rosa_laevigata RLG00000031635 RLG00000031641
rosa_multiflora Rmu_sc0006233.1_g000030
rosa_roxburghii Rroxscaffold_1G00066840 Rroxscaffold_1G00067540
rosa_rugosa Rorug04G0441600
rosa_samantha Rh5AG071400 Rh5BG067900 Rh5CG080200 Rh5DG067900
rosa_wichuraiana Rw5G006670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 5222
AccB1I GGYRCC 4 cut(s) 289, 592, 904, 3896
AccB7I CCANNNNNTGG 2 cut(s) 634, 5160
AccI GTMKAC 1 cut(s) 937
AccII CGCG 6 cut(s) 868, 1254, 3558, 4365, 4671, 5306
AclWI GGATC 9 cut(s) 560, 983, 1628, 1759, 2390, 2976, 3449, 4377, 4390
AcoI YGGCCR 3 cut(s) 2427, 3121, 4116
AcsI RAATTY 7 cut(s) 56, 2439, 2460, 3471, 3757, 4024, 4744
AcuI CTGAAG 8 cut(s) 635, 1050, 2024, 3537, 4215, 5109, 5229, 5325
AdeI CACNNNGTG 1 cut(s) 3212
AfaI GTAC 8 cut(s) 2373, 2418, 3099, 3742, 4102, 4395, 4589, 4609
AflII CTTAAG 1 cut(s) 2993
AflIII ACRYGT 1 cut(s) 4042
AgeI ACCGGT 1 cut(s) 2160
AhdI GACNNNNNGTC 1 cut(s) 4300
AjiI CACGTC 1 cut(s) 4643
AjnI CCWGG 5 cut(s) 1861, 2170, 2429, 3457, 4118
AjuI GAANNNNNNNTTGG 6 cut(s) 2723, 2755, 2990, 3022, 3036, 3068
Alw21I GWGCWC 3 cut(s) 489, 3779, 3801
AlwI GGATC 9 cut(s) 560, 983, 1628, 1759, 2390, 2976, 3449, 4377, 4390
AlwNI CAGNNNCTG 1 cut(s) 3176
Ama87I CYCGRG 3 cut(s) 128, 1691, 3658
ApoI RAATTY 7 cut(s) 56, 2439, 2460, 3471, 3757, 4024, 4744
ArsI GACNNNNNNTTYG 2 cut(s) 2519, 2551
AseI ATTAAT 2 cut(s) 1041, 4139
AsiGI ACCGGT 1 cut(s) 2160
Asp700I GAANNNNTTC 4 cut(s) 1359, 2089, 2253, 4251
AspLEI GCGC 2 cut(s) 1254, 5078
AsuC2I CCSGG 2 cut(s) 342, 1129
AsuHPI GGTGA 5 cut(s) 1772, 2852, 4604, 4705, 4973
AvaI CYCGRG 3 cut(s) 128, 1691, 3658
AvaII GGWCC 7 cut(s) 263, 687, 788, 1125, 2275, 3249, 3384
AxyI CCTNAGG 1 cut(s) 3892
BaeGI GKGCMC 2 cut(s) 595, 909
BalI TGGCCA 1 cut(s) 4118
BamHI GGATCC 1 cut(s) 4382
BanI GGYRCC 4 cut(s) 289, 592, 904, 3896
BanII GRGCYC 6 cut(s) 365, 489, 3779, 3801, 4340, 4491
BarI GAAGNNNNNNTAC 2 cut(s) 4239, 4271
BauI CACGAG 1 cut(s) 4591
BbsI GAAGAC 6 cut(s) 110, 869, 2538, 4203, 4605, 5097
Bbv12I GWGCWC 3 cut(s) 489, 3779, 3801
BbvCI CCTCAGC 3 cut(s) 2409, 3253, 4926
BccI CCATC 9 cut(s) 779, 799, 923, 1776, 2593, 4005, 5138, 5154, 5315
BceAI ACGGC 5 cut(s) 814, 1600, 3136, 3558, 4851
BciT130I CCWGG 5 cut(s) 1863, 2172, 2431, 3459, 4120
BciVI GTATCC 3 cut(s) 142, 4398, 5194
BcnI CCSGG 2 cut(s) 342, 1129
BfaI CTAG 7 cut(s) 417, 1463, 1973, 2154, 2748, 3702, 5184
BfmI CTRYAG 6 cut(s) 597, 2094, 3177, 4256, 4419, 5350
BfrI CTTAAG 1 cut(s) 2993
BfuI GTATCC 3 cut(s) 142, 4398, 5194
BglII AGATCT 2 cut(s) 2150, 2239
BlpI GCTNAGC 1 cut(s) 177
BmcAI AGTACT 1 cut(s) 4609
Bme1390I CCNGG 7 cut(s) 342, 1129, 1863, 2172, 2431, 3459, 4120
Bme18I GGWCC 7 cut(s) 263, 687, 788, 1125, 2275, 3249, 3384
BmeRI GACNNNNNGTC 1 cut(s) 4300
BmeT110I CYCGRG 3 cut(s) 128, 1691, 3658
BmgBI CACGTC 1 cut(s) 4643
BmrFI CCNGG 7 cut(s) 342, 1129, 1863, 2172, 2431, 3459, 4120
BmrI ACTGGG 2 cut(s) 4343, 5137
BmuI ACTGGG 2 cut(s) 4343, 5137
BpiI GAAGAC 6 cut(s) 110, 869, 2538, 4203, 4605, 5097
BpmI CTGGAG 5 cut(s) 706, 1160, 1884, 3153, 3520
Bpu10I CCTNAGC 5 cut(s) 303, 2409, 3253, 4485, 4926
Bpu1102I GCTNAGC 1 cut(s) 177
BpuEI CTTGAG 8 cut(s) 361, 696, 1421, 1801, 2231, 2984, 3853, 4115
BpuMI CCSGG 2 cut(s) 342, 1129
Bsa29I ATCGAT 3 cut(s) 2394, 2971, 3418
BsaBI GATNNNNATC 1 cut(s) 2556
BsaJI CCNNGG 9 cut(s) 144, 683, 853, 1616, 3199, 3273, 4079, 4113, 4400
BsaWI WCCGGW 3 cut(s) 1006, 2160, 3107
Bse118I RCCGGY 2 cut(s) 2160, 3686
Bse21I CCTNAGG 1 cut(s) 3892
Bse3DI GCAATG 1 cut(s) 495
Bse8I GATNNNNATC 1 cut(s) 2556
BseBI CCWGG 5 cut(s) 1863, 2172, 2431, 3459, 4120
BseCI ATCGAT 3 cut(s) 2394, 2971, 3418
BseDI CCNNGG 9 cut(s) 144, 683, 853, 1616, 3199, 3273, 4079, 4113, 4400
BseJI GATNNNNATC 1 cut(s) 2556
BseMI GCAATG 1 cut(s) 495
BseRI GAGGAG 8 cut(s) 466, 1174, 3769, 4564, 4665, 4840, 4865, 4917
BseSI GKGCMC 2 cut(s) 595, 909
BsgI GTGCAG 2 cut(s) 1380, 1398
Bsh1236I CGCG 6 cut(s) 868, 1254, 3558, 4365, 4671, 5306
Bsh1285I CGRYCG 1 cut(s) 208
BshNI GGYRCC 4 cut(s) 289, 592, 904, 3896
BshTI ACCGGT 1 cut(s) 2160
BshVI ATCGAT 3 cut(s) 2394, 2971, 3418
BsiEI CGRYCG 1 cut(s) 208
BsiHKAI GWGCWC 3 cut(s) 489, 3779, 3801
BsiHKCI CYCGRG 3 cut(s) 128, 1691, 3658
BslFI GGGAC 7 cut(s) 962, 2238, 2288, 3114, 3370, 4800, 5161
BsmBI CGTCTC 2 cut(s) 1378, 2520
BsmFI GGGAC 7 cut(s) 962, 2238, 2288, 3114, 3370, 4800, 5161
BsmI GAATGC 5 cut(s) 1547, 1568, 2738, 3878, 4234
BsoBI CYCGRG 3 cut(s) 128, 1691, 3658
Bsp1286I GDGCHC 8 cut(s) 365, 489, 595, 909, 3779, 3801, 4340, 4491
Bsp1720I GCTNAGC 1 cut(s) 177
Bsp19I CCATGG 3 cut(s) 853, 3199, 3273
Bsp68I TCGCGA 1 cut(s) 4365
BspDI ATCGAT 3 cut(s) 2394, 2971, 3418
BspFNI CGCG 6 cut(s) 868, 1254, 3558, 4365, 4671, 5306
BspHI TCATGA 6 cut(s) 40, 655, 1114, 1735, 2560, 4441
BspMAI CTGCAG 3 cut(s) 2098, 3181, 4423
BspPI GGATC 9 cut(s) 560, 983, 1628, 1759, 2390, 2976, 3449, 4377, 4390
BspQI GCTCTTC 3 cut(s) 1350, 2889, 4182
BspT107I GGYRCC 4 cut(s) 289, 592, 904, 3896
BspTI CTTAAG 1 cut(s) 2993
BsrDI GCAATG 1 cut(s) 495
BsrFI RCCGGY 2 cut(s) 2160, 3686
BssAI RCCGGY 2 cut(s) 2160, 3686
BssECI CCNNGG 9 cut(s) 144, 683, 853, 1616, 3199, 3273, 4079, 4113, 4400
BssSI CACGAG 1 cut(s) 4591
BssT1I CCWWGG 8 cut(s) 144, 683, 853, 3199, 3273, 4079, 4113, 4400
Bst2BI CACGAG 1 cut(s) 4591
Bst2UI CCWGG 5 cut(s) 1863, 2172, 2431, 3459, 4120
BstAFI CTTAAG 1 cut(s) 2993
BstAPI GCANNNNNTGC 2 cut(s) 904, 1982
BstDSI CCRYGG 4 cut(s) 853, 1616, 3199, 3273
BstEII GGTNACC 1 cut(s) 1643
BstENI CCTNNNNNAGG 3 cut(s) 3462, 3835, 4117
BstFNI CGCG 6 cut(s) 868, 1254, 3558, 4365, 4671, 5306
BstHHI GCGC 2 cut(s) 1254, 5078
BstMCI CGRYCG 1 cut(s) 208
BstNI CCWGG 5 cut(s) 1863, 2172, 2431, 3459, 4120
BstNSI RCATGY 1 cut(s) 4046
BstPI GGTNACC 1 cut(s) 1643
BstSCI CCNGG 7 cut(s) 340, 1127, 1861, 2170, 2429, 3457, 4118
BstSFI CTRYAG 6 cut(s) 597, 2094, 3177, 4256, 4419, 5350
BstSLI GKGCMC 2 cut(s) 595, 909
BstUI CGCG 6 cut(s) 868, 1254, 3558, 4365, 4671, 5306
BstV2I GAAGAC 6 cut(s) 110, 869, 2538, 4203, 4605, 5097
BstX2I RGATCY 4 cut(s) 1764, 2150, 2239, 4382
BstXI CCANNNNNNTGG 1 cut(s) 4711
BstYI RGATCY 4 cut(s) 1764, 2150, 2239, 4382
Bsu15I ATCGAT 3 cut(s) 2394, 2971, 3418
Bsu36I CCTNAGG 1 cut(s) 3892
BsuI GTATCC 3 cut(s) 142, 4398, 5194
BsuTUI ATCGAT 3 cut(s) 2394, 2971, 3418
BtgI CCRYGG 4 cut(s) 853, 1616, 3199, 3273
BtgZI GCGATG 2 cut(s) 2679, 5321
BtrI CACGTC 1 cut(s) 4643
BtsI GCAGTG 5 cut(s) 3174, 3982, 4416, 4491, 5112
BtuMI TCGCGA 1 cut(s) 4365
CaiI CAGNNNCTG 1 cut(s) 3176
CciI TCATGA 6 cut(s) 40, 655, 1114, 1735, 2560, 4441
CfoI GCGC 2 cut(s) 1254, 5078
Cfr10I RCCGGY 2 cut(s) 2160, 3686
ClaI ATCGAT 3 cut(s) 2394, 2971, 3418
CseI GACGC 7 cut(s) 83, 641, 852, 1395, 2888, 4221, 4677
Csp6I GTAC 8 cut(s) 2372, 2417, 3098, 3741, 4101, 4394, 4588, 4608
CspAI ACCGGT 1 cut(s) 2160
CviQI GTAC 8 cut(s) 2372, 2417, 3098, 3741, 4101, 4394, 4588, 4608
DraIII CACNNNGTG 1 cut(s) 3212
DrdI GACNNNNNNGTC 1 cut(s) 5222
DriI GACNNNNNGTC 1 cut(s) 4300
DseDI GACNNNNNNGTC 1 cut(s) 5222
EaeI YGGCCR 3 cut(s) 2427, 3121, 4116
Eam1105I GACNNNNNGTC 1 cut(s) 4300
Ecl136II GAGCTC 3 cut(s) 487, 3777, 3799
Eco130I CCWWGG 8 cut(s) 144, 683, 853, 3199, 3273, 4079, 4113, 4400
Eco147I AGGCCT 1 cut(s) 4578
Eco24I GRGCYC 6 cut(s) 365, 489, 3779, 3801, 4340, 4491
Eco47I GGWCC 7 cut(s) 263, 687, 788, 1125, 2275, 3249, 3384
Eco53kI GAGCTC 3 cut(s) 487, 3777, 3799
Eco57I CTGAAG 8 cut(s) 635, 1050, 2024, 3537, 4215, 5109, 5229, 5325
Eco81I CCTNAGG 1 cut(s) 3892
Eco88I CYCGRG 3 cut(s) 128, 1691, 3658
Eco91I GGTNACC 1 cut(s) 1643
EcoICRI GAGCTC 3 cut(s) 487, 3777, 3799
EcoNI CCTNNNNNAGG 3 cut(s) 3462, 3835, 4117
EcoO109I RGGNCCY 5 cut(s) 687, 2275, 3249, 3384, 3522
EcoO65I GGTNACC 1 cut(s) 1643
EcoRI GAATTC 1 cut(s) 3757
EcoRII CCWGG 5 cut(s) 1861, 2170, 2429, 3457, 4118
EcoT14I CCWWGG 8 cut(s) 144, 683, 853, 3199, 3273, 4079, 4113, 4400
EcoT22I ATGCAT 7 cut(s) 586, 742, 1549, 1903, 2110, 2740, 3316
EcoT38I GRGCYC 6 cut(s) 365, 489, 3779, 3801, 4340, 4491
ErhI CCWWGG 8 cut(s) 144, 683, 853, 3199, 3273, 4079, 4113, 4400
Esp3I CGTCTC 2 cut(s) 1378, 2520
FalI AAGNNNNNCTT 8 cut(s) 95, 127, 1383, 1415, 2886, 2918, 4280, 4312
FaqI GGGAC 7 cut(s) 962, 2238, 2288, 3114, 3370, 4800, 5161
FauI CCCGC 3 cut(s) 301, 552, 3551
FauNDI CATATG 5 cut(s) 139, 742, 1897, 3310, 4465
FblI GTMKAC 1 cut(s) 937
FriOI GRGCYC 6 cut(s) 365, 489, 3779, 3801, 4340, 4491
FspBI CTAG 7 cut(s) 417, 1463, 1973, 2154, 2748, 3702, 5184
GlaI GCGC 2 cut(s) 1253, 5077
GsuI CTGGAG 5 cut(s) 706, 1160, 1884, 3153, 3520
HgaI GACGC 7 cut(s) 83, 641, 852, 1395, 2888, 4221, 4677
HhaI GCGC 2 cut(s) 1254, 5078
Hin6I GCGC 2 cut(s) 1252, 5076
HinP1I GCGC 2 cut(s) 1252, 5076
HincII GTYRAC 1 cut(s) 2166
HindII GTYRAC 1 cut(s) 2166
HindIII AAGCTT 1 cut(s) 2301
HphI GGTGA 5 cut(s) 1772, 2852, 4604, 4705, 4973
Hpy166II GTNNAC 9 cut(s) 266, 938, 1871, 2166, 2372, 4040, 4590, 4625, 4799
Hpy8I GTNNAC 9 cut(s) 266, 938, 1871, 2166, 2372, 4040, 4590, 4625, 4799
Hpy99I CGWCG 5 cut(s) 163, 868, 4206, 4725, 5099
HpyCH4IV ACGT 6 cut(s) 1285, 1580, 3096, 4070, 4642, 5223
HpySE526I ACGT 6 cut(s) 1285, 1580, 3096, 4070, 4642, 5223
HspAI GCGC 2 cut(s) 1252, 5076
KroI GCCGGC 1 cut(s) 3686
KroNI GCCGGC 1 cut(s) 3688
LguI GCTCTTC 3 cut(s) 1350, 2889, 4182
MaeI CTAG 7 cut(s) 417, 1463, 1973, 2154, 2748, 3702, 5184
MaeII ACGT 6 cut(s) 1285, 1580, 3096, 4070, 4642, 5223
MfeI CAATTG 1 cut(s) 515
MflI RGATCY 4 cut(s) 1764, 2150, 2239, 4382
MhlI GDGCHC 8 cut(s) 365, 489, 595, 909, 3779, 3801, 4340, 4491
MlsI TGGCCA 1 cut(s) 4118
MluNI TGGCCA 1 cut(s) 4118
MmeI TCCRAC 9 cut(s) 141, 165, 799, 2650, 3605, 4053, 4703, 4977, 5313
Mox20I TGGCCA 1 cut(s) 4118
Mph1103I ATGCAT 7 cut(s) 586, 742, 1549, 1903, 2110, 2740, 3316
MroNI GCCGGC 1 cut(s) 3686
MroXI GAANNNNTTC 4 cut(s) 1359, 2089, 2253, 4251
MscI TGGCCA 1 cut(s) 4118
MslI CAYNNNNRTG 4 cut(s) 440, 858, 1740, 1900
Msp20I TGGCCA 1 cut(s) 4118
MspA1I CMGCKG 9 cut(s) 280, 613, 1484, 2002, 2099, 2426, 3022, 4424, 5157
MspCI CTTAAG 1 cut(s) 2993
MspR9I CCNGG 7 cut(s) 342, 1129, 1863, 2172, 2431, 3459, 4120
MunI CAATTG 1 cut(s) 515
Mva1269I GAATGC 5 cut(s) 1547, 1568, 2738, 3878, 4234
MvaI CCWGG 5 cut(s) 1863, 2172, 2431, 3459, 4120
MvnI CGCG 6 cut(s) 868, 1254, 3558, 4365, 4671, 5306
NaeI GCCGGC 1 cut(s) 3688
NciI CCSGG 2 cut(s) 342, 1129
NcoI CCATGG 3 cut(s) 853, 3199, 3273
NdeI CATATG 5 cut(s) 139, 742, 1897, 3310, 4465
NgoMIV GCCGGC 1 cut(s) 3686
NmuCI GTSAC 8 cut(s) 230, 2719, 3172, 3204, 3324, 4874, 4961, 5186
NruI TCGCGA 1 cut(s) 4365
NsiI ATGCAT 7 cut(s) 586, 742, 1549, 1903, 2110, 2740, 3316
NspI RCATGY 1 cut(s) 4046
PaeR7I CTCGAG 2 cut(s) 128, 1691
PagI TCATGA 6 cut(s) 40, 655, 1114, 1735, 2560, 4441
PceI AGGCCT 1 cut(s) 4578
PciI ACATGT 1 cut(s) 4042
PciSI GCTCTTC 3 cut(s) 1350, 2889, 4182
PcsI WCGNNNNNNNCGW 3 cut(s) 126, 473, 4807
PctI GAATGC 5 cut(s) 1547, 1568, 2738, 3878, 4234
PdiI GCCGGC 1 cut(s) 3688
PdmI GAANNNNTTC 4 cut(s) 1359, 2089, 2253, 4251
PflFI GACNNNGTC 1 cut(s) 4803
PflMI CCANNNNNTGG 2 cut(s) 634, 5160
PfoI TCCNGGA 2 cut(s) 1861, 2170
PinAI ACCGGT 1 cut(s) 2160
Ple19I CGATCG 1 cut(s) 208
PpuMI RGGWCCY 4 cut(s) 687, 2275, 3249, 3384
PscI ACATGT 1 cut(s) 4042
PshBI ATTAAT 2 cut(s) 1041, 4139
Psp124BI GAGCTC 3 cut(s) 489, 3779, 3801
Psp5II RGGWCCY 4 cut(s) 687, 2275, 3249, 3384
Psp6I CCWGG 5 cut(s) 1861, 2170, 2429, 3457, 4118
PspEI GGTNACC 1 cut(s) 1643
PspGI CCWGG 5 cut(s) 1861, 2170, 2429, 3457, 4118
PspPPI RGGWCCY 4 cut(s) 687, 2275, 3249, 3384
PstI CTGCAG 3 cut(s) 2098, 3181, 4423
PstNI CAGNNNCTG 1 cut(s) 3176
PsuI RGATCY 4 cut(s) 1764, 2150, 2239, 4382
PsyI GACNNNGTC 1 cut(s) 4803
PvuI CGATCG 1 cut(s) 208
PvuII CAGCTG 6 cut(s) 613, 1484, 2002, 2099, 3022, 4424
RruI TCGCGA 1 cut(s) 4365
RsaI GTAC 8 cut(s) 2373, 2418, 3099, 3742, 4102, 4395, 4589, 4609
RsaNI GTAC 8 cut(s) 2372, 2417, 3098, 3741, 4101, 4394, 4588, 4608
RseI CAYNNNNRTG 4 cut(s) 440, 858, 1740, 1900
SacI GAGCTC 3 cut(s) 489, 3779, 3801
SapI GCTCTTC 3 cut(s) 1350, 2889, 4182
ScaI AGTACT 1 cut(s) 4609
ScrFI CCNGG 7 cut(s) 342, 1129, 1863, 2172, 2431, 3459, 4120
SduI GDGCHC 8 cut(s) 365, 489, 595, 909, 3779, 3801, 4340, 4491
SfcI CTRYAG 6 cut(s) 597, 2094, 3177, 4256, 4419, 5350
Sfr274I CTCGAG 2 cut(s) 128, 1691
SinI GGWCC 7 cut(s) 263, 687, 788, 1125, 2275, 3249, 3384
SlaI CTCGAG 2 cut(s) 128, 1691
SmiMI CAYNNNNRTG 4 cut(s) 440, 858, 1740, 1900
SseBI AGGCCT 1 cut(s) 4578
SspI AATATT 3 cut(s) 1639, 1962, 2876
SspMI CTAG 7 cut(s) 417, 1463, 1973, 2154, 2748, 3702, 5184
SstI GAGCTC 3 cut(s) 489, 3779, 3801
StuI AGGCCT 1 cut(s) 4578
StyD4I CCNGG 7 cut(s) 340, 1127, 1861, 2170, 2429, 3457, 4118
StyI CCWWGG 8 cut(s) 144, 683, 853, 3199, 3273, 4079, 4113, 4400
TaiI ACGT 6 cut(s) 1288, 1583, 3099, 4073, 4645, 5226
TaqII GACCGA 1 cut(s) 720
TatI WGTACW 6 cut(s) 2416, 3740, 4100, 4393, 4587, 4607
TauI GCSGC 4 cut(s) 11, 577, 2429, 2664
TseFI GTSAC 8 cut(s) 230, 2719, 3172, 3204, 3324, 4874, 4961, 5186
Tsp45I GTSAC 8 cut(s) 230, 2719, 3172, 3204, 3324, 4874, 4961, 5186
TspGWI ACGGA 1 cut(s) 4375
Tth111I GACNNNGTC 1 cut(s) 4803
Van91I CCANNNNNTGG 2 cut(s) 634, 5160
Vha464I CTTAAG 1 cut(s) 2993
VpaK11BI GGWCC 7 cut(s) 263, 687, 788, 1125, 2275, 3249, 3384
VspI ATTAAT 2 cut(s) 1041, 4139
XagI CCTNNNNNAGG 3 cut(s) 3462, 3835, 4117
XapI RAATTY 7 cut(s) 56, 2439, 2460, 3471, 3757, 4024, 4744
XbaI TCTAGA 1 cut(s) 2153
XceI RCATGY 1 cut(s) 4046
XcmI CCANNNNNNNNNTGG 2 cut(s) 502, 4423
XhoI CTCGAG 2 cut(s) 128, 1691
XmiI GTMKAC 1 cut(s) 937
XmnI GAANNNNTTC 4 cut(s) 1359, 2089, 2253, 4251
XspI CTAG 7 cut(s) 417, 1463, 1973, 2154, 2748, 3702, 5184
ZrmI AGTACT 1 cut(s) 4609
Zsp2I ATGCAT 7 cut(s) 586, 742, 1549, 1903, 2110, 2740, 3316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.