Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0004714

Physical Location & Seq

Genomic Coordinates Reverse (-)

28470162 .. 28474152

Gene Structure 3991 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2694 bp

ATGGAAGTTTTGATAGTAGAACAAGGGCGGCTGGAGCGGTATAACGATAAAGATATTAGGGTGCCTAAGAGAAGACGTTCCAATTTCCTGCATCATGGAAAGGGAGGCGACAAATCAGAAGAGGATGAAAAGCACAAGGACTTCTCATCATTGACTGGAACAGAGTCTAAAAAGAGGCGGCTTACTCCAGAGAATGGAAAAAGGATTTCGAAAAAGCGACGCTCCTCCTCAAAAGTGGTAGAAAGTTCTGAAGATGAGTTTGATGGGGAAATTCTGTTTCTTGTTAAGGCACAAGCGCGCAAAAGGGGAGGAGTTGACTGTGAAGTGATAGGAAGAAGTTCCTTAAAGGATGGAACAGTAAGATATGAGTCAAGGAATGGAGCTTGCAATACCTCGTCTTCTTCACCACCGACTTCTCCTGGATCAACTTCAAGAGATATGAAGCAGGATAATGAAGAAGTTAATTTAAAGTGTCATCAGTGTATGAAAGAGGAGAAGAAGACTATTCTTTCCTGCTCCAAATGTAAAAAGAACTCCTATTGCATCCTCTGTATCAAGCAATGGTATCCTCACATGAAAGTAAAGGAAGTCAAGGAACTTTGCCCATTTTGCCGCAAGAATTGCAACTGTAATGCATGCCTGCACTCAACTGGTGTGATTAAGATACCAGAGACGGATTTAGATGACCGCAAAAAGGCTCAGCATCTGGAGCATTTACTTTCCAACTTGCTTCCATTTCTAAAGAAAATTTCCCAAGAACAAAATCAGGAGATAGAGATTGAAGCTAATCTTCGAGGGCTTTCACCTTCTGCAGTTGAAATACCGCAGACTCTCTGTTTTAATGATGAGCGTGTCTATTGCAACCACTGTGCAACTTCAATCATTGATCTACACCGAAGCTGCTCAAAATGTTCGTATGAACTTTGTCTAAATTGCTGCCAAGAAATTCGCCAGGGATGTCTTTTTGACCGTGGAGACATGAAATTTCAGTATAGGAGTAGGGGTTATGATTACATCCATGGTGGAGACCCGGCACCTGACTGTTGTCCCTTGAAAGAGTCAGAGGACCATATTAAGCCTTTGACTGAGTGGAAGGCAAACAGTGATGCCACTGTTAGTTGTGCTCCGAAAGAAATGGGTGGCTGTGGTGACTGCGTGTTGGATCTCAAGCGCATCCTTCCGGCCGACTGTGTCTCAAATTTAGTAGTGAAAGCAAAATATTTATTAGAGACTTTCCAAAAAGAGCGTTCTACATTCAAGCATAATTGTGAAACAAGAAGAGATATGTTACGAAAAGCAGCTTCCCGAGAAGGTTCTTGTGACAACTTCTTGTTTTGCCCTGATTCAAAGGACACTCTGAAAGAAGAAGAGCTTTTGCACTTTAAAGAACATTGGGTGAATGGTGAACCAGTTATTGTTAAAAATGTGCTCGAACAGGCAAATGGTCTGAGCTGGGAACCAATGGTAATGTGGCGTGCTCTAAGTGAAAATATGGATGCAACTAGTACACAATATTCAGAAGTAAAGACCATCGATTGCCTGGCTGGTTGTGAGGTGGAAATCAACACTCGTCAGTTTTTTCAAGGATACACTGAAGGGAGAATGTACAAAAACTTGTGGCCAGAGATGCTTAAGCTGAAGGATTGGCCCCCTTCTGATAAGTTTGAGGATCTTTCACCGCGTCATTGTGATGAATTTATCAGTGCCTTGCCATTTCTAGAGTACACAACGGCTGAGATCGATCCTAGAGCTGGTTTCCTTAACCTTGCTGTTAAGTTGCCGCGAGGTGTCTTGAAACCAGACATGGGTCCAAAAACATATATTGCTTATGGTTTTATGGAAGAGCTTGGAAGAGGAGACTCCGTAACTAAGCTCCATTGTGACATGTCTGATGCGGTGAATATTTTGACACATACCGCTGAAGTGCAGTCAAGTAATGAGCAGCAGTGTGCAATTTCTAGGTTGAAAAAGTTACATAGGGTGCAAGATGAAAGAGAATTTATGGATTGGGAGAATTCTCACAAGGATCGTGCTGGACAGTCTGGTGGACAGACAGAGGACAGAGAAACCCTAGACAGCAATTGCCCATCTGAAATTAATGGAGAATTGAAAGTTCGGAAAAATGAACTAGATGATTCGTCTTGTTCTTCCTCCAATGAAGCAACCGAGGAAACGGGTGGTGCTTTGTGGGACATCTTCCGCAGGGAGGATGTCCCTAAGTTAGAGGCTTATCTGATGAAGCATTATAAAGAATTTAGGCACACCTATTGCTCACTAGTTGAAAAGGTCATTCATCCAATTCATGACCAGTCTTTTTATTTAACGTTGGAGCACAAGCGGAGGCTGAAGGAGGAATTTGGTGTCGAACCATGGACTTTTGTACAAAGACTCGGAGAGGCGGTATTTATTCCAGCCGGATGCCCACACCAAGTCAGGAATCTCAAGTCGTGCACGAAAGTAGCAGCAGACTTCGTATCTCCTGAAAATGTCCATGAGTGCCTCCGTTTAACTGAGGAGTTTAGACAACTTCCGAAGAACCATAGAGCCAGAGAAGACAAACTTGAGATCAAGAAAATGATACTTTACGCTGTCGATCAAGCTGTTAAAGATTTTGAATCCTTGGCATCGACTCAAGAGTTGGAAGCCTTGGTATCAACTCGAGGTTTGAAGGCCTTGGAATCAACTGAAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008214 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031326 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0033169 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1903506 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04714 map04714

Pfam Domains

Protein Families

JmjC zf-4CXXC_R1

Protein Analysis

898

Amino Acids

102.66

Weight (kDa)

6.27

Isoelectric Point (pI)

54.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
JmjC	PF02373	725 - 824	2e-13	JmjC domain, hydroxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004714)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11950 AT1G11950 AT1G11950 AT1G62310
fragaria_vesca	FvH4_3g01550 FvH4_3g01550 FvH4_3g01550 FvH4_3g01550 FvH4_3g01550 FvH4_3g01550 FvH4_3g01550 FvH4_3g01550
malus_domestica	MD05G1351300.v1.1 MD10G1325700.v1.1
prunus_persica	Prupe.4G016300_v2.0.a1
pyrus_communis	pycom05g31840 pycom10g27540
rosa_chinensis	RchiOBHm_Chr5g0002371
rosa_laevigata	RLG00000031023
rosa_multiflora	Rmu_co8351855.1_g000001 Rmu_co8445921.1_g000001 Rmu_sc0006137.1_g000004 Rmu_sc0006137.1_g000005 Rmu_sc0017945.1_g000004
rosa_rugosa	Rorug04G0395600 Rorug04G0395700.1
rosa_samantha	Rh5AG018400 Rh5BG021100 Rh5CG020300 Rh5DG019300
rosa_wichuraiana	Rw5G001730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2247
AccB1I	GGYRCC	2 cut(s)	61, 1033
AccB7I	CCANNNNNTGG	1 cut(s)	194
AccBSI	CCGCTC	1 cut(s)	37
AccII	CGCG	3 cut(s)	298, 1681, 1783
AclI	AACGTT	1 cut(s)	2324
AclWI	GGATC	5 cut(s)	430, 1170, 1677, 1736, 2034
AcoI	YGGCCR	2 cut(s)	1182, 1619
AcuI	CTGAAG	5 cut(s)	270, 1614, 1658, 1941, 2366
AfaI	GTAC	4 cut(s)	1507, 1607, 1724, 2382
AfiI	CCNNNNNNNGG	5 cut(s)	194, 694, 1751, 1805, 2206
AflII	CTTAAG	1 cut(s)	1631
AflIII	ACRYGT	1 cut(s)	1884
AhlI	ACTAGT	2 cut(s)	1502, 2275
AjnI	CCWGG	3 cut(s)	418, 951, 1539
Alw21I	GWGCWC	5 cut(s)	1126, 1431, 1480, 2334, 2453
Alw26I	GTCTC	6 cut(s)	665, 969, 1020, 1198, 1223, 1851
Alw44I	GTGCAC	1 cut(s)	2449
AlwI	GGATC	5 cut(s)	430, 1170, 1677, 1736, 2034
AlwNI	CAGNNNCTG	1 cut(s)	706
Ama87I	CYCGRG	2 cut(s)	1305, 2658
AoxI	GGCC	4 cut(s)	1182, 1619, 1646, 2670
ApaLI	GTGCAC	1 cut(s)	2449
ApeKI	GCWGC	5 cut(s)	900, 936, 1298, 1942, 2462
AseI	ATTAAT	1 cut(s)	2097
Asp700I	GAANNNNTTC	2 cut(s)	76, 337
AspLEI	GCGC	3 cut(s)	298, 300, 1173
AspS9I	GGNCC	3 cut(s)	1066, 1647, 1808
AsuC2I	CCSGG	1 cut(s)	1031
AsuHPI	GGTGA	7 cut(s)	396, 795, 1160, 1408, 1415, 1668, 1909
AsuII	TTCGAA	1 cut(s)	209
AvaI	CYCGRG	2 cut(s)	1305, 2658
AvaII	GGWCC	2 cut(s)	1066, 1808
BaeGI	GKGCMC	1 cut(s)	2453
BalI	TGGCCA	1 cut(s)	1621
BanI	GGYRCC	2 cut(s)	61, 1033
BbsI	GAAGAC	4 cut(s)	79, 390, 506, 2559
Bbv12I	GWGCWC	5 cut(s)	1126, 1431, 1480, 2334, 2453
BbvI	GCAGC	5 cut(s)	887, 923, 1310, 1954, 2474
BccI	CCATC	4 cut(s)	257, 344, 1538, 2095
BceAI	ACGGC	1 cut(s)	1746
BciT130I	CCWGG	3 cut(s)	420, 953, 1541
BciVI	GTATCC	2 cut(s)	576, 1580
BcnI	CCSGG	1 cut(s)	1031
BcoDI	GTCTC	6 cut(s)	665, 969, 1020, 1198, 1223, 1851
BcuI	ACTAGT	2 cut(s)	1502, 2275
BfaI	CTAG	7 cut(s)	1503, 1718, 1746, 1959, 2072, 2129, 2276
BfmI	CTRYAG	1 cut(s)	810
BfrI	CTTAAG	1 cut(s)	1631
BfuI	GTATCC	2 cut(s)	576, 1580
BisI	GCNGC	9 cut(s)	29, 179, 613, 901, 937, 1299, 1781, 1943, 2463
BlpI	GCTNAGC	1 cut(s)	699
BlsI	GCNGC	9 cut(s)	30, 180, 614, 902, 938, 1300, 1782, 1944, 2464
Bme1390I	CCNGG	4 cut(s)	420, 953, 1031, 1541
Bme18I	GGWCC	2 cut(s)	1066, 1808
BmeT110I	CYCGRG	2 cut(s)	1305, 2658
BmgT120I	GGNCC	3 cut(s)	1066, 1647, 1808
BmiI	GGNNCC	5 cut(s)	63, 1035, 1458, 1649, 1809
BmrFI	CCNGG	4 cut(s)	420, 953, 1031, 1541
BoxI	GACNNNNGTC	2 cut(s)	1044, 1806
BpiI	GAAGAC	4 cut(s)	79, 390, 506, 2559
BpmI	CTGGAG	3 cut(s)	53, 171, 728
Bpu1102I	GCTNAGC	1 cut(s)	699
Bpu14I	TTCGAA	1 cut(s)	209
BpuEI	CTTGAG	4 cut(s)	1151, 2426, 2582, 2616
BpuMI	CCSGG	1 cut(s)	1031
Bsa29I	ATCGAT	2 cut(s)	1533, 1740
BsaI	GGTCTC	1 cut(s)	1020
BsaJI	CCNNGG	8 cut(s)	952, 970, 1018, 2166, 2369, 2619, 2646, 2673
BsaXI	ACNNNNNCTCC	6 cut(s)	988, 1018, 1848, 1878, 2385, 2415
Bsc4I	CCNNNNNNNGG	5 cut(s)	194, 694, 1751, 1805, 2206
Bse1I	ACTGG	4 cut(s)	160, 655, 1409, 2308
Bse3DI	GCAATG	1 cut(s)	566
BseBI	CCWGG	3 cut(s)	420, 953, 1541
BseCI	ATCGAT	2 cut(s)	1533, 1740
BseDI	CCNNGG	8 cut(s)	952, 970, 1018, 2166, 2369, 2619, 2646, 2673
BseLI	CCNNNNNNNGG	5 cut(s)	194, 694, 1751, 1805, 2206
BseMI	GCAATG	1 cut(s)	566
BseMII	CTCAG	5 cut(s)	713, 1077, 1439, 1725, 2502
BseNI	ACTGG	4 cut(s)	160, 655, 1409, 2308
BsePI	GCGCGC	1 cut(s)	296
BseRI	GAGGAG	6 cut(s)	214, 217, 324, 506, 1869, 2528
BseSI	GKGCMC	1 cut(s)	2453
BseX3I	CGGCCG	1 cut(s)	1182
BseXI	GCAGC	5 cut(s)	887, 923, 1310, 1954, 2474
BseYI	CCCAGC	1 cut(s)	1452
BsgI	GTGCAG	2 cut(s)	626, 1946
Bsh1236I	CGCG	3 cut(s)	298, 1681, 1783
Bsh1285I	CGRYCG	1 cut(s)	1185
BshFI	GGCC	4 cut(s)	1184, 1621, 1648, 2672
BshNI	GGYRCC	2 cut(s)	61, 1033
BshVI	ATCGAT	2 cut(s)	1533, 1740
BsiEI	CGRYCG	1 cut(s)	1185
BsiHKAI	GWGCWC	5 cut(s)	1126, 1431, 1480, 2334, 2453
BsiHKCI	CYCGRG	2 cut(s)	1305, 2658
BsiSI	CCGG	3 cut(s)	1031, 1181, 2415
BslFI	GGGAC	3 cut(s)	1032, 2198, 2204
BslI	CCNNNNNNNGG	5 cut(s)	194, 694, 1751, 1805, 2206
BsmAI	GTCTC	6 cut(s)	665, 969, 1020, 1198, 1223, 1851
BsmBI	CGTCTC	1 cut(s)	665
BsmFI	GGGAC	3 cut(s)	1032, 2198, 2204
BsnI	GGCC	4 cut(s)	1184, 1621, 1648, 2672
Bso31I	GGTCTC	1 cut(s)	1020
BsoBI	CYCGRG	2 cut(s)	1305, 2658
Bsp119I	TTCGAA	1 cut(s)	209
Bsp1286I	GDGCHC	5 cut(s)	1126, 1431, 1480, 2334, 2453
Bsp1407I	TGTACA	2 cut(s)	1605, 2380
Bsp143I	GATC	9 cut(s)	422, 886, 1162, 1669, 1737, 1741, 2026, 2565, 2593
Bsp1720I	GCTNAGC	1 cut(s)	699
Bsp19I	CCATGG	2 cut(s)	1018, 2369
BspANI	GGCC	4 cut(s)	1184, 1621, 1648, 2672
BspCNI	CTCAG	5 cut(s)	712, 1078, 1440, 1726, 2503
BspDI	ATCGAT	2 cut(s)	1533, 1740
BspFNI	CGCG	3 cut(s)	298, 1681, 1783
BspHI	TCATGA	1 cut(s)	2302
BspLI	GGNNCC	5 cut(s)	63, 1035, 1458, 1649, 1809
BspMAI	CTGCAG	1 cut(s)	814
BspPI	GGATC	5 cut(s)	430, 1170, 1677, 1736, 2034
BspQI	GCTCTTC	2 cut(s)	1362, 1836
BspT104I	TTCGAA	1 cut(s)	209
BspT107I	GGYRCC	2 cut(s)	61, 1033
BspTI	CTTAAG	1 cut(s)	1631
BspTNI	GGTCTC	1 cut(s)	1020
BsrBI	CCGCTC	1 cut(s)	37
BsrDI	GCAATG	1 cut(s)	566
BsrGI	TGTACA	2 cut(s)	1605, 2380
BsrI	ACTGG	4 cut(s)	160, 655, 1409, 2308
BssECI	CCNNGG	8 cut(s)	952, 970, 1018, 2166, 2369, 2619, 2646, 2673
BssHII	GCGCGC	1 cut(s)	296
BssMI	GATC	9 cut(s)	422, 886, 1162, 1669, 1737, 1741, 2026, 2565, 2593
BssT1I	CCWWGG	5 cut(s)	1018, 2369, 2619, 2646, 2673
Bst2UI	CCWGG	3 cut(s)	420, 953, 1541
Bst6I	CTCTTC	5 cut(s)	114, 1273, 1362, 1836, 1846
BstAFI	CTTAAG	1 cut(s)	1631
BstAPI	GCANNNNNTGC	1 cut(s)	621
BstAUI	TGTACA	2 cut(s)	1605, 2380
BstBI	TTCGAA	1 cut(s)	209
BstC8I	GCNNGC	5 cut(s)	298, 385, 637, 641, 1476
BstDEI	CTNAG	9 cut(s)	66, 699, 1086, 1448, 1481, 1734, 1869, 2217, 2511
BstDSI	CCRYGG	3 cut(s)	970, 1018, 2369
BstFNI	CGCG	3 cut(s)	298, 1681, 1783
BstHHI	GCGC	3 cut(s)	298, 300, 1173
BstKTI	GATC	9 cut(s)	425, 889, 1165, 1672, 1740, 1744, 2029, 2568, 2596
BstMAI	GTCTC	6 cut(s)	665, 969, 1020, 1198, 1223, 1851
BstMBI	GATC	9 cut(s)	422, 886, 1162, 1669, 1737, 1741, 2026, 2565, 2593
BstMCI	CGRYCG	1 cut(s)	1185
BstMWI	GCNNNNNNNGC	5 cut(s)	34, 609, 621, 709, 1627
BstNI	CCWGG	3 cut(s)	420, 953, 1541
BstNSI	RCATGY	2 cut(s)	639, 1888
BstPAI	GACNNNNGTC	2 cut(s)	1044, 1806
BstSCI	CCNGG	4 cut(s)	418, 951, 1029, 1539
BstSFI	CTRYAG	1 cut(s)	810
BstSLI	GKGCMC	1 cut(s)	2453
BstUI	CGCG	3 cut(s)	298, 1681, 1783
BstV1I	GCAGC	5 cut(s)	887, 923, 1310, 1954, 2474
BstV2I	GAAGAC	4 cut(s)	79, 390, 506, 2559
BstX2I	RGATCY	2 cut(s)	1162, 1669
BstYI	RGATCY	2 cut(s)	1162, 1669
BstZI	CGGCCG	1 cut(s)	1182
Bsu15I	ATCGAT	2 cut(s)	1533, 1740
BsuI	GTATCC	2 cut(s)	576, 1580
BsuRI	GGCC	4 cut(s)	1184, 1621, 1648, 2672
BsuTUI	ATCGAT	2 cut(s)	1533, 1740
BtgI	CCRYGG	3 cut(s)	970, 1018, 2369
BtsI	GCAGTG	1 cut(s)	1952
BtsIMutI	CAGTG	7 cut(s)	485, 865, 1108, 1110, 1590, 1708, 1952
Cac8I	GCNNGC	5 cut(s)	298, 385, 637, 641, 1476
CaiI	CAGNNNCTG	1 cut(s)	706
CciI	TCATGA	1 cut(s)	2302
CfoI	GCGC	3 cut(s)	298, 300, 1173
Cfr13I	GGNCC	3 cut(s)	1066, 1647, 1808
ClaI	ATCGAT	2 cut(s)	1533, 1740
CseI	GACGC	2 cut(s)	228, 1670
Csp6I	GTAC	4 cut(s)	1506, 1606, 1723, 2381
CviQI	GTAC	4 cut(s)	1506, 1606, 1723, 2381
DdeI	CTNAG	9 cut(s)	66, 699, 1086, 1448, 1481, 1734, 1869, 2217, 2511
DpnI	GATC	9 cut(s)	424, 888, 1164, 1671, 1739, 1743, 2028, 2567, 2595
DpnII	GATC	9 cut(s)	422, 886, 1162, 1669, 1737, 1741, 2026, 2565, 2593
DraI	TTTAAA	2 cut(s)	468, 1384
EaeI	YGGCCR	2 cut(s)	1182, 1619
EagI	CGGCCG	1 cut(s)	1182
Eam1104I	CTCTTC	5 cut(s)	114, 1273, 1362, 1836, 1846
EarI	CTCTTC	5 cut(s)	114, 1273, 1362, 1836, 1846
EclXI	CGGCCG	1 cut(s)	1182
Eco130I	CCWWGG	5 cut(s)	1018, 2369, 2619, 2646, 2673
Eco147I	AGGCCT	1 cut(s)	2672
Eco31I	GGTCTC	1 cut(s)	1020
Eco47I	GGWCC	2 cut(s)	1066, 1808
Eco52I	CGGCCG	1 cut(s)	1182
Eco57I	CTGAAG	5 cut(s)	270, 1614, 1658, 1941, 2366
Eco88I	CYCGRG	2 cut(s)	1305, 2658
EcoRI	GAATTC	1 cut(s)	2014
EcoRII	CCWGG	3 cut(s)	418, 951, 1539
EcoT14I	CCWWGG	5 cut(s)	1018, 2369, 2619, 2646, 2673
EcoT22I	ATGCAT	1 cut(s)	637
ErhI	CCWWGG	5 cut(s)	1018, 2369, 2619, 2646, 2673
Esp3I	CGTCTC	1 cut(s)	665
FalI	AAGNNNNNCTT	6 cut(s)	774, 806, 1356, 1388, 2544, 2576
FaqI	GGGAC	3 cut(s)	1032, 2198, 2204
Fnu4HI	GCNGC	9 cut(s)	29, 179, 613, 901, 937, 1299, 1781, 1943, 2463
Fsp4HI	GCNGC	9 cut(s)	29, 179, 613, 901, 937, 1299, 1781, 1943, 2463
FspBI	CTAG	7 cut(s)	1503, 1718, 1746, 1959, 2072, 2129, 2276
GlaI	GCGC	3 cut(s)	297, 299, 1172
GluI	GCNGC	9 cut(s)	29, 179, 613, 901, 937, 1299, 1781, 1943, 2463
GsaI	CCCAGC	1 cut(s)	1456
GsuI	CTGGAG	3 cut(s)	53, 171, 728
HaeIII	GGCC	4 cut(s)	1184, 1621, 1648, 2672
HapII	CCGG	3 cut(s)	1031, 1181, 2415
HgaI	GACGC	2 cut(s)	228, 1670
HhaI	GCGC	3 cut(s)	298, 300, 1173
Hin6I	GCGC	3 cut(s)	296, 298, 1171
HinP1I	GCGC	3 cut(s)	296, 298, 1171
HincII	GTYRAC	1 cut(s)	316
HindII	GTYRAC	1 cut(s)	316
HpaII	CCGG	3 cut(s)	1031, 1181, 2415
HphI	GGTGA	7 cut(s)	396, 795, 1160, 1408, 1415, 1668, 1909
Hpy166II	GTNNAC	6 cut(s)	316, 1406, 1508, 1725, 2048, 2451
Hpy8I	GTNNAC	6 cut(s)	316, 1406, 1508, 1725, 2048, 2451
Hpy99I	CGWCG	1 cut(s)	222
HpyAV	CCTTC	9 cut(s)	816, 1087, 1187, 1304, 1589, 1633, 1662, 2341, 2662
HpyCH4IV	ACGT	2 cut(s)	76, 2324
HpyF10VI	GCNNNNNNNGC	5 cut(s)	34, 609, 621, 709, 1627
HpyF3I	CTNAG	9 cut(s)	66, 699, 1086, 1448, 1481, 1734, 1869, 2217, 2511
HpySE526I	ACGT	2 cut(s)	76, 2324
HspAI	GCGC	3 cut(s)	296, 298, 1171
Kzo9I	GATC	9 cut(s)	422, 886, 1162, 1669, 1737, 1741, 2026, 2565, 2593
LguI	GCTCTTC	2 cut(s)	1362, 1836
LmnI	GCTCC	8 cut(s)	34, 227, 380, 521, 709, 1129, 1878, 2329
Lsp1109I	GCAGC	5 cut(s)	887, 923, 1310, 1954, 2474
MaeI	CTAG	7 cut(s)	1503, 1718, 1746, 1959, 2072, 2129, 2276
MaeII	ACGT	2 cut(s)	76, 2324
MaeIII	GTNAC	6 cut(s)	1148, 1287, 1319, 1864, 1880, 1971
MalI	GATC	9 cut(s)	424, 888, 1164, 1671, 1739, 1743, 2028, 2567, 2595
MbiI	CCGCTC	1 cut(s)	37
MboI	GATC	9 cut(s)	422, 886, 1162, 1669, 1737, 1741, 2026, 2565, 2593
MfeI	CAATTG	1 cut(s)	2080
MflI	RGATCY	2 cut(s)	1162, 1669
MhlI	GDGCHC	5 cut(s)	1126, 1431, 1480, 2334, 2453
MlsI	TGGCCA	1 cut(s)	1621
MluNI	TGGCCA	1 cut(s)	1621
MlyI	GAGTC	7 cut(s)	173, 377, 823, 1067, 1853, 2382, 2623
MmeI	TCCRAC	4 cut(s)	747, 1140, 2307, 2619
Mox20I	TGGCCA	1 cut(s)	1621
Mph1103I	ATGCAT	1 cut(s)	637
MroXI	GAANNNNTTC	2 cut(s)	76, 337
MscI	TGGCCA	1 cut(s)	1621
MslI	CAYNNNNRTG	2 cut(s)	1266, 1689
Msp20I	TGGCCA	1 cut(s)	1621
MspA1I	CMGCKG	1 cut(s)	1919
MspCI	CTTAAG	1 cut(s)	1631
MspI	CCGG	3 cut(s)	1031, 1181, 2415
MspR9I	CCNGG	4 cut(s)	420, 953, 1031, 1541
MunI	CAATTG	1 cut(s)	2080
MvaI	CCWGG	3 cut(s)	420, 953, 1541
MvnI	CGCG	3 cut(s)	298, 1681, 1783
MwoI	GCNNNNNNNGC	5 cut(s)	34, 609, 621, 709, 1627
NciI	CCSGG	1 cut(s)	1031
NcoI	CCATGG	2 cut(s)	1018, 2369
NdeII	GATC	9 cut(s)	422, 886, 1162, 1669, 1737, 1741, 2026, 2565, 2593
NlaIV	GGNNCC	5 cut(s)	63, 1035, 1458, 1649, 1809
NmuCI	GTSAC	3 cut(s)	1148, 1319, 1880
NsiI	ATGCAT	1 cut(s)	637
NspI	RCATGY	2 cut(s)	639, 1888
NspV	TTCGAA	1 cut(s)	209
PaeI	GCATGC	1 cut(s)	639
PaeR7I	CTCGAG	1 cut(s)	2658
PagI	TCATGA	1 cut(s)	2302
PauI	GCGCGC	1 cut(s)	296
PceI	AGGCCT	1 cut(s)	2672
PciI	ACATGT	1 cut(s)	1884
PciSI	GCTCTTC	2 cut(s)	1362, 1836
PdmI	GAANNNNTTC	2 cut(s)	76, 337
PfeI	GAWTC	5 cut(s)	1343, 2135, 2437, 2615, 2678
PflFI	GACNNNGTC	1 cut(s)	1190
PflMI	CCANNNNNTGG	1 cut(s)	194
PfoI	TCCNGGA	1 cut(s)	418
PkrI	GCNGC	9 cut(s)	30, 180, 614, 902, 938, 1300, 1782, 1944, 2464
PleI	GAGTC	7 cut(s)	172, 376, 823, 1066, 1853, 2382, 2623
PpsI	GAGTC	7 cut(s)	172, 376, 823, 1066, 1853, 2382, 2623
PscI	ACATGT	1 cut(s)	1884
PshAI	GACNNNNGTC	2 cut(s)	1044, 1806
PshBI	ATTAAT	1 cut(s)	2097
PsiI	TTATAA	1 cut(s)	2247
Psp1406I	AACGTT	1 cut(s)	2324
Psp6I	CCWGG	3 cut(s)	418, 951, 1539
PspFI	CCCAGC	1 cut(s)	1452
PspGI	CCWGG	3 cut(s)	418, 951, 1539
PspN4I	GGNNCC	5 cut(s)	63, 1035, 1458, 1649, 1809
PspPI	GGNCC	3 cut(s)	1066, 1647, 1808
PspXI	VCTCGAGB	1 cut(s)	2658
PstI	CTGCAG	1 cut(s)	814
PstNI	CAGNNNCTG	1 cut(s)	706
PsuI	RGATCY	2 cut(s)	1162, 1669
PsyI	GACNNNGTC	1 cut(s)	1190
PteI	GCGCGC	1 cut(s)	296
RsaI	GTAC	4 cut(s)	1507, 1607, 1724, 2382
RsaNI	GTAC	4 cut(s)	1506, 1606, 1723, 2381
RseI	CAYNNNNRTG	2 cut(s)	1266, 1689
SapI	GCTCTTC	2 cut(s)	1362, 1836
SatI	GCNGC	9 cut(s)	29, 179, 613, 901, 937, 1299, 1781, 1943, 2463
Sau3AI	GATC	9 cut(s)	422, 886, 1162, 1669, 1737, 1741, 2026, 2565, 2593
Sau96I	GGNCC	3 cut(s)	1066, 1647, 1808
SchI	GAGTC	7 cut(s)	173, 377, 823, 1067, 1853, 2382, 2623
ScrFI	CCNGG	4 cut(s)	420, 953, 1031, 1541
SduI	GDGCHC	5 cut(s)	1126, 1431, 1480, 2334, 2453
SfcI	CTRYAG	1 cut(s)	810
Sfr274I	CTCGAG	1 cut(s)	2658
SfuI	TTCGAA	1 cut(s)	209
SinI	GGWCC	2 cut(s)	1066, 1808
SlaI	CTCGAG	1 cut(s)	2658
SmiMI	CAYNNNNRTG	2 cut(s)	1266, 1689
SmlI	CTYRAG	6 cut(s)	1166, 1631, 2441, 2561, 2631, 2658
SmoI	CTYRAG	6 cut(s)	1166, 1631, 2441, 2561, 2631, 2658
SpeI	ACTAGT	2 cut(s)	1502, 2275
SphI	GCATGC	1 cut(s)	639
SseBI	AGGCCT	1 cut(s)	2672
SspI	AATATT	3 cut(s)	1220, 1514, 1903
SspMI	CTAG	7 cut(s)	1503, 1718, 1746, 1959, 2072, 2129, 2276
StuI	AGGCCT	1 cut(s)	2672
StyD4I	CCNGG	4 cut(s)	418, 951, 1029, 1539
StyI	CCWWGG	5 cut(s)	1018, 2369, 2619, 2646, 2673
TaiI	ACGT	2 cut(s)	79, 2327
TaqI	TCGA	9 cut(s)	209, 793, 1431, 1533, 1740, 2364, 2592, 2627, 2659
TatI	WGTACW	4 cut(s)	1505, 1605, 1722, 2380
TauI	GCSGC	4 cut(s)	31, 181, 615, 1783
TfiI	GAWTC	5 cut(s)	1343, 2135, 2437, 2615, 2678
TscAI	CASTG	7 cut(s)	485, 872, 1108, 1117, 1597, 1708, 1952
TseFI	GTSAC	3 cut(s)	1148, 1319, 1880
TseI	GCWGC	5 cut(s)	900, 936, 1298, 1942, 2462
Tsp45I	GTSAC	3 cut(s)	1148, 1319, 1880
TspGWI	ACGGA	3 cut(s)	689, 1852, 2492
TspRI	CASTG	7 cut(s)	485, 872, 1108, 1117, 1597, 1708, 1952
Tth111I	GACNNNGTC	1 cut(s)	1190
Van91I	CCANNNNNTGG	1 cut(s)	194
Vha464I	CTTAAG	1 cut(s)	1631
VneI	GTGCAC	1 cut(s)	2449
VpaK11BI	GGWCC	2 cut(s)	1066, 1808
VspI	ATTAAT	1 cut(s)	2097
XbaI	TCTAGA	1 cut(s)	1717
XceI	RCATGY	2 cut(s)	639, 1888
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1467, 1537
XhoI	CTCGAG	1 cut(s)	2658
XmnI	GAANNNNTTC	2 cut(s)	76, 337
XspI	CTAG	7 cut(s)	1503, 1718, 1746, 1959, 2072, 2129, 2276
Zsp2I	ATGCAT	1 cut(s)	637

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.