pycom10g29180

Protein ENDOSPERM DEFECTIVE 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0005101
Physical Location & Seq
Reverse (-)
29575723 .. 29578742
3020 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1647 bp
ATGGATCAATTGATCGTGGAAAATTGCGCATCTGCCACCGTAGAGGACGCAGAAGTTTGCGCTCCTCCCCATCCTCCTCCTCCTCCACCACCGACTGCTCAAAGGCGGCCAAGAGTGAGGGAGGTGAGCTCTAGGTTCATGTCTCCTGCGGCTTTCACCGGTGATCTCCCTCATCCCTTGCCTTCCAAATCGCCCCTTCCTAAACATCAACACACCATCTTAACTCCATCCTCGACCCTGTCGGAAATTCAGTCGAGGCAGCGGTCTTCCTCTGTGAATAGGCGGCAGAGGCACTTGGACTTGGAGCCCTTGCGCTCCTCCGACGAGAACAGGCCCACCGGATCCTCCTCCATTCACACTCAGAGTCAGATCTGGGGAACCCCTCTCCCACCGGACCAGATGCTTAATTTTAGGAAACAGCGTCCTGCCAAGCCTTTGAAAGAGAACGGAGGAGGAGGAGGAGGCAAAACCCAGCAGCAGCAGAAGCAGCATCTGCCAAAAACCGCACCCTCAAGACCTGACACACCAGTGGCGAGTGCTAGTCTAGATAGGATGTCGAGAGTCAGACTCACTCAGCAGCGGTCTACCAACATAACTGCCACAGCTGCTGCTAAGCTCTTGCAGGCCAGCGGGATGTCTTTACCTGCTCCGCCTGCCACTGCAGTTACCGAGAGTGACACCACTTGCCCGGATGTCAATAATCATAGTCAAACGCTAAGCTGTACCGCACGGTGTCTTCCTGACATTCGTTCTTCAATGCCTGAGGCTGATTTGTTGCCAACCGTTTCTAGCAGGCAGCTCTCTGAGAAAAGTAGCAGCAGGGGTAATGCAACTGTCGGTGCCAGTGGCGATTTTTCAAAGTATTCTGCTTCCCCTTGCTCCCGTTCTCTGAATTTGTCATTATCAAGTTCCGACCGCTTATTCTTCCTGACAGACAAAGGCAGTGAAAGAGGGAAGGTGGGTGGCCTATGCCTTCCCCCAGTCCCACCATGTGCTAGTGCAAAGCTGGGCCCAGATACAAGGAAAGGAAAGAAAGTTTCCAGTCATCTAGAAGATGTACATTCCTTGAGAGTGCTCCACAACCGCTATCTGCAGTGGAGATATGCCAATGCAAGAGCAGAGGCTTCCATGCGAGCTCAGCAGAGAGAGACCCAGAGAACACTTTATTCTCTCGCGGTAAAGATTGCTGAACTATATGATTCTGTGAAGAGGAAACGGATAGAACTCAGCATTTTGCAAAGAACAAAGACTTTATCCACAATTCTTGAAGCTCAAATTCCATATTTGGACCAGTGGTTTTCTCTTGAAGGGGATTATTCAGTTTCTCTGGCAGAAGCAACTCAAGCATTGTTGAATGCCTCAGTTCAACTTCCAAGCAGTGGCAGTGTTAGGGCCAATCTACGAGAGTTAGAAGAGGCACTGAGCACAGCAATAGAAGTGATGGACATAATAGTTTTCCATGTTCAGCGATCTATGCCAAAGGCAGAAGATACAGAACATTTGATTTCGGAACTAGCCAGAGTGATTGGTGGAGAAAGAGCTCTCATTGAAGAATGCGGAAATCTGCTGTCTAAGACATATACAACGCAGGTGGAAGAGTGGAGCTTAAGAAGCCAGATAATCCAATCGAAATTATTTCTTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

549

Amino Acids

60.1

Weight (kDa)

9.34

Isoelectric Point (pI)

63.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 242 - 517 5.6e-51 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1579
Acc16I TGCGCA 1 cut(s) 28
Acc36I ACCTGC 2 cut(s) 652, 1579
AccB1I GGYRCC 1 cut(s) 839
AccB7I CCANNNNNTGG 1 cut(s) 1379
AccI GTMKAC 1 cut(s) 584
AccII CGCG 1 cut(s) 1175
AclWI GGATC 3 cut(s) 12, 336, 349
AcoI YGGCCR 1 cut(s) 107
AcsI RAATTY 3 cut(s) 246, 892, 1275
AdeI CACNNNGTG 1 cut(s) 992
AfaI GTAC 2 cut(s) 724, 1059
AfiI CCNNNNNNNGG 1 cut(s) 1379
AflII CTTAAG 1 cut(s) 1606
AgeI ACCGGT 1 cut(s) 158
AgsI TTSAA 8 cut(s) 439, 756, 858, 1268, 1307, 1354, 1367, 1550
AleI CACNNNNGTG 1 cut(s) 527
Alw21I GWGCWC 5 cut(s) 131, 1077, 1138, 1427, 1543
Alw26I GTCTC 2 cut(s) 147, 1142
AlwI GGATC 3 cut(s) 12, 336, 349
AlwNI CAGNNNCTG 2 cut(s) 493, 608
AoxI GGCC 6 cut(s) 107, 332, 624, 964, 1009, 1392
ApaI GGGCCC 1 cut(s) 1013
ApeKI GCWGC 9 cut(s) 259, 475, 478, 487, 577, 605, 608, 796, 816
ApoI RAATTY 3 cut(s) 246, 892, 1275
AsiGI ACCGGT 1 cut(s) 158
AspLEI GCGC 3 cut(s) 29, 62, 315
AspS9I GGNCC 6 cut(s) 333, 394, 1009, 1010, 1288, 1392
AsuC2I CCSGG 1 cut(s) 689
AsuHPI GGTGA 3 cut(s) 136, 148, 173
AvaII GGWCC 2 cut(s) 394, 1288
AxyI CCTNAGG 1 cut(s) 762
BaeGI GKGCMC 1 cut(s) 1013
BamHI GGATCC 1 cut(s) 341
BanI GGYRCC 1 cut(s) 839
BanII GRGCYC 5 cut(s) 131, 309, 1013, 1138, 1543
BbsI GAAGAC 2 cut(s) 258, 728
Bbv12I GWGCWC 5 cut(s) 131, 1077, 1138, 1427, 1543
BbvI GCAGC 9 cut(s) 271, 487, 490, 499, 589, 592, 595, 808, 828
BccI CCATC 4 cut(s) 78, 224, 235, 1435
BcnI CCSGG 1 cut(s) 689
BcoDI GTCTC 2 cut(s) 147, 1142
BfaI CTAG 7 cut(s) 132, 540, 545, 789, 996, 1049, 1514
BfmI CTRYAG 2 cut(s) 660, 1091
BfrI CTTAAG 1 cut(s) 1606
BfuAI ACCTGC 2 cut(s) 652, 1579
BglII AGATCT 1 cut(s) 369
BlpI GCTNAGC 3 cut(s) 612, 716, 1137
Bme1390I CCNGG 1 cut(s) 689
Bme18I GGWCC 2 cut(s) 394, 1288
BmgT120I GGNCC 6 cut(s) 333, 394, 1009, 1010, 1288, 1392
BmiI GGNNCC 5 cut(s) 306, 343, 379, 841, 1011
BmrFI CCNGG 1 cut(s) 689
BmrI ACTGGG 1 cut(s) 974
BmsI GCATC 3 cut(s) 38, 390, 499
BmuI ACTGGG 1 cut(s) 974
BpiI GAAGAC 2 cut(s) 258, 728
BplI GAGNNNNNCTC 4 cut(s) 113, 145, 552, 584
Bpu1102I GCTNAGC 3 cut(s) 612, 716, 1137
BpuEI CTTGAG 3 cut(s) 496, 1087, 1326
BpuMI CCSGG 1 cut(s) 689
BsaI GGTCTC 1 cut(s) 1142
BsaWI WCCGGW 3 cut(s) 158, 338, 391
Bsc4I CCNNNNNNNGG 1 cut(s) 1379
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 5 cut(s) 527, 843, 980, 1041, 1291
Bse21I CCTNAGG 1 cut(s) 762
BseGI GGATG 6 cut(s) 70, 172, 227, 558, 639, 697
BseLI CCNNNNNNNGG 1 cut(s) 1379
BseMII CTCAG 8 cut(s) 374, 587, 753, 795, 1151, 1240, 1374, 1412
BseNI ACTGG 5 cut(s) 527, 843, 980, 1041, 1291
BseSI GKGCMC 1 cut(s) 1013
BseXI GCAGC 9 cut(s) 271, 487, 490, 499, 589, 592, 595, 808, 828
BseYI CCCAGC 2 cut(s) 471, 1006
Bsh1236I CGCG 1 cut(s) 1175
Bsh1285I CGRYCG 1 cut(s) 916
BshFI GGCC 6 cut(s) 109, 334, 626, 966, 1011, 1394
BshNI GGYRCC 1 cut(s) 839
BshTI ACCGGT 1 cut(s) 158
BsiEI CGRYCG 1 cut(s) 916
BsiHKAI GWGCWC 5 cut(s) 131, 1077, 1138, 1427, 1543
BsiSI CCGG 4 cut(s) 159, 339, 392, 689
BslFI GGGAC 1 cut(s) 968
BslI CCNNNNNNNGG 1 cut(s) 1379
BsmAI GTCTC 2 cut(s) 147, 1142
BsmFI GGGAC 1 cut(s) 968
BsmI GAATGC 2 cut(s) 1360, 1559
BsnI GGCC 6 cut(s) 109, 334, 626, 966, 1011, 1394
Bso31I GGTCTC 1 cut(s) 1142
Bsp120I GGGCCC 1 cut(s) 1009
Bsp1286I GDGCHC 7 cut(s) 131, 309, 1013, 1077, 1138, 1427, 1543
Bsp1407I TGTACA 1 cut(s) 1057
Bsp143I GATC 6 cut(s) 4, 12, 163, 341, 369, 1469
Bsp1720I GCTNAGC 3 cut(s) 612, 716, 1137
BspANI GGCC 6 cut(s) 109, 334, 626, 966, 1011, 1394
BspCNI CTCAG 8 cut(s) 373, 586, 754, 796, 1150, 1239, 1373, 1413
BspFNI CGCG 1 cut(s) 1175
BspLI GGNNCC 5 cut(s) 306, 343, 379, 841, 1011
BspMAI CTGCAG 2 cut(s) 664, 1095
BspMI ACCTGC 2 cut(s) 652, 1579
BspPI GGATC 3 cut(s) 12, 336, 349
BspT107I GGYRCC 1 cut(s) 839
BspTI CTTAAG 1 cut(s) 1606
BspTNI GGTCTC 1 cut(s) 1142
BsrFI RCCGGY 1 cut(s) 158
BsrGI TGTACA 1 cut(s) 1057
BsrI ACTGG 5 cut(s) 527, 843, 980, 1041, 1291
BssAI RCCGGY 1 cut(s) 158
BssMI GATC 6 cut(s) 4, 12, 163, 341, 369, 1469
Bst4CI ACNGT 4 cut(s) 40, 732, 784, 835
Bst6I CTCTTC 3 cut(s) 1202, 1407, 1590
BstAFI CTTAAG 1 cut(s) 1606
BstAPI GCANNNNNTGC 1 cut(s) 493
BstAUI TGTACA 1 cut(s) 1057
BstC8I GCNNGC 5 cut(s) 624, 628, 654, 794, 1134
BstF5I GGATG 6 cut(s) 70, 172, 227, 558, 639, 697
BstFNI CGCG 1 cut(s) 1175
BstHHI GCGC 3 cut(s) 29, 62, 315
BstKTI GATC 6 cut(s) 7, 15, 166, 344, 372, 1472
BstMAI GTCTC 2 cut(s) 147, 1142
BstMBI GATC 6 cut(s) 4, 12, 163, 341, 369, 1469
BstMCI CGRYCG 1 cut(s) 916
BstSCI CCNGG 1 cut(s) 687
BstSFI CTRYAG 2 cut(s) 660, 1091
BstSLI GKGCMC 1 cut(s) 1013
BstUI CGCG 1 cut(s) 1175
BstV1I GCAGC 9 cut(s) 271, 487, 490, 499, 589, 592, 595, 808, 828
BstV2I GAAGAC 2 cut(s) 258, 728
BstX2I RGATCY 2 cut(s) 341, 369
BstYI RGATCY 2 cut(s) 341, 369
Bsu36I CCTNAGG 1 cut(s) 762
BsuRI GGCC 6 cut(s) 109, 334, 626, 966, 1011, 1394
BtsCI GGATG 6 cut(s) 70, 172, 227, 558, 639, 697
BtsI GCAGTG 5 cut(s) 657, 949, 1100, 1384, 1390
BtsIMutI CAGTG 9 cut(s) 534, 657, 850, 949, 1100, 1298, 1384, 1390, 1418
BveI ACCTGC 2 cut(s) 652, 1579
Cac8I GCNNGC 5 cut(s) 624, 628, 654, 794, 1134
CaiI CAGNNNCTG 2 cut(s) 493, 608
CfoI GCGC 3 cut(s) 29, 62, 315
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 6 cut(s) 333, 394, 1009, 1010, 1288, 1392
CseI GACGC 2 cut(s) 56, 410
Csp6I GTAC 2 cut(s) 723, 1058
CspAI ACCGGT 1 cut(s) 158
CspCI CAANNNNNGTGG 4 cut(s) 1246, 1281, 1572, 1607
CviAII CATG 4 cut(s) 139, 990, 1129, 1460
CviQI GTAC 2 cut(s) 723, 1058
DpnI GATC 6 cut(s) 6, 14, 165, 343, 371, 1471
DpnII GATC 6 cut(s) 4, 12, 163, 341, 369, 1469
DraIII CACNNNGTG 1 cut(s) 992
EaeI YGGCCR 1 cut(s) 107
Eam1104I CTCTTC 3 cut(s) 1202, 1407, 1590
EarI CTCTTC 3 cut(s) 1202, 1407, 1590
EciI GGCGGA 1 cut(s) 639
Ecl136II GAGCTC 3 cut(s) 129, 1136, 1541
Eco24I GRGCYC 5 cut(s) 131, 309, 1013, 1138, 1543
Eco31I GGTCTC 1 cut(s) 1142
Eco47I GGWCC 2 cut(s) 394, 1288
Eco53kI GAGCTC 3 cut(s) 129, 1136, 1541
Eco81I CCTNAGG 1 cut(s) 762
EcoICRI GAGCTC 3 cut(s) 129, 1136, 1541
EcoT38I GRGCYC 5 cut(s) 131, 309, 1013, 1138, 1543
FaeI CATG 4 cut(s) 142, 993, 1132, 1463
FaqI GGGAC 1 cut(s) 968
FatI CATG 4 cut(s) 138, 989, 1128, 1459
FauI CCCGC 1 cut(s) 623
FblI GTMKAC 1 cut(s) 584
FokI GGATG 6 cut(s) 57, 159, 214, 565, 646, 704
FriOI GRGCYC 5 cut(s) 131, 309, 1013, 1138, 1543
FspBI CTAG 7 cut(s) 132, 540, 545, 789, 996, 1049, 1514
FspI TGCGCA 1 cut(s) 28
GlaI GCGC 3 cut(s) 28, 61, 314
GsaI CCCAGC 2 cut(s) 475, 1010
HaeIII GGCC 6 cut(s) 109, 334, 626, 966, 1011, 1394
HapII CCGG 4 cut(s) 159, 339, 392, 689
HgaI GACGC 2 cut(s) 56, 410
HhaI GCGC 3 cut(s) 29, 62, 315
Hin1II CATG 4 cut(s) 142, 993, 1132, 1463
Hin6I GCGC 3 cut(s) 27, 60, 313
HinP1I GCGC 3 cut(s) 27, 60, 313
HinfI GANTC 4 cut(s) 364, 561, 567, 1199
HpaII CCGG 4 cut(s) 159, 339, 392, 689
HphI GGTGA 3 cut(s) 136, 148, 173
Hpy166II GTNNAC 1 cut(s) 585
Hpy188I TCNGA 9 cut(s) 244, 322, 363, 369, 566, 805, 891, 913, 1510
Hpy188III TCNNGA 8 cut(s) 513, 545, 558, 740, 928, 1049, 1265, 1304
Hpy8I GTNNAC 1 cut(s) 585
Hpy99I CGWCG 1 cut(s) 326
HpyAV CCTTC 5 cut(s) 192, 206, 949, 983, 1301
HpyCH4III ACNGT 4 cut(s) 40, 732, 784, 835
HpyCH4V TGCA 7 cut(s) 622, 662, 830, 1001, 1093, 1112, 1237
Hsp92II CATG 4 cut(s) 142, 993, 1132, 1463
HspAI GCGC 3 cut(s) 27, 60, 313
Kzo9I GATC 6 cut(s) 4, 12, 163, 341, 369, 1469
LmnI GCTCC 7 cut(s) 67, 304, 320, 652, 884, 1080, 1602
Lsp1109I GCAGC 9 cut(s) 271, 487, 490, 499, 589, 592, 595, 808, 828
LweI GCATC 3 cut(s) 38, 390, 499
MaeI CTAG 7 cut(s) 132, 540, 545, 789, 996, 1049, 1514
MaeIII GTNAC 2 cut(s) 664, 674
MalI GATC 6 cut(s) 6, 14, 165, 343, 371, 1471
MboI GATC 6 cut(s) 4, 12, 163, 341, 369, 1469
MfeI CAATTG 1 cut(s) 8
MflI RGATCY 2 cut(s) 341, 369
MhlI GDGCHC 7 cut(s) 131, 309, 1013, 1077, 1138, 1427, 1543
MluCI AATT 8 cut(s) 8, 22, 246, 406, 892, 1260, 1275, 1631
MlyI GAGTC 3 cut(s) 373, 561, 570
MmeI TCCRAC 3 cut(s) 222, 345, 936
MseI TTAA 3 cut(s) 221, 405, 1607
MslI CAYNNNNRTG 1 cut(s) 527
MspA1I CMGCKG 4 cut(s) 262, 580, 605, 630
MspCI CTTAAG 1 cut(s) 1606
MspI CCGG 4 cut(s) 159, 339, 392, 689
MspR9I CCNGG 1 cut(s) 689
MunI CAATTG 1 cut(s) 8
Mva1269I GAATGC 2 cut(s) 1360, 1559
MvnI CGCG 1 cut(s) 1175
NciI CCSGG 1 cut(s) 689
NdeII GATC 6 cut(s) 4, 12, 163, 341, 369, 1469
NlaIII CATG 4 cut(s) 142, 993, 1132, 1463
NlaIV GGNNCC 5 cut(s) 306, 343, 379, 841, 1011
NmuCI GTSAC 1 cut(s) 674
NsbI TGCGCA 1 cut(s) 28
OliI CACNNNNGTG 1 cut(s) 527
PaqCI CACCTGC 1 cut(s) 1579
PctI GAATGC 2 cut(s) 1360, 1559
PfeI GAWTC 1 cut(s) 1199
PflFI GACNNNGTC 1 cut(s) 238
PflMI CCANNNNNTGG 1 cut(s) 1379
PinAI ACCGGT 1 cut(s) 158
PleI GAGTC 3 cut(s) 372, 561, 569
PpsI GAGTC 3 cut(s) 372, 561, 569
Psp124BI GAGCTC 3 cut(s) 131, 1138, 1543
PspFI CCCAGC 2 cut(s) 471, 1006
PspN4I GGNNCC 5 cut(s) 306, 343, 379, 841, 1011
PspOMI GGGCCC 1 cut(s) 1009
PspPI GGNCC 6 cut(s) 333, 394, 1009, 1010, 1288, 1392
PstI CTGCAG 2 cut(s) 664, 1095
PstNI CAGNNNCTG 2 cut(s) 493, 608
PsuI RGATCY 2 cut(s) 341, 369
PsyI GACNNNGTC 1 cut(s) 238
PvuII CAGCTG 1 cut(s) 605
RsaI GTAC 2 cut(s) 724, 1059
RsaNI GTAC 2 cut(s) 723, 1058
RseI CAYNNNNRTG 1 cut(s) 527
SacI GAGCTC 3 cut(s) 131, 1138, 1543
SaqAI TTAA 3 cut(s) 221, 405, 1607
Sau3AI GATC 6 cut(s) 4, 12, 163, 341, 369, 1469
Sau96I GGNCC 6 cut(s) 333, 394, 1009, 1010, 1288, 1392
SchI GAGTC 3 cut(s) 373, 561, 570
ScrFI CCNGG 1 cut(s) 689
SduI GDGCHC 7 cut(s) 131, 309, 1013, 1077, 1138, 1427, 1543
SfaNI GCATC 3 cut(s) 38, 390, 499
SfcI CTRYAG 2 cut(s) 660, 1091
SgrAI CRCCGGYG 1 cut(s) 158
SinI GGWCC 2 cut(s) 394, 1288
SmiMI CAYNNNNRTG 1 cut(s) 527
SmlI CTYRAG 4 cut(s) 511, 1066, 1341, 1606
SmoI CTYRAG 4 cut(s) 511, 1066, 1341, 1606
Sse9I AATT 8 cut(s) 8, 22, 246, 406, 892, 1260, 1275, 1631
SspMI CTAG 7 cut(s) 132, 540, 545, 789, 996, 1049, 1514
SstI GAGCTC 3 cut(s) 131, 1138, 1543
StyD4I CCNGG 1 cut(s) 687
TaaI ACNGT 4 cut(s) 40, 732, 784, 835
TaqI TCGA 4 cut(s) 233, 254, 557, 1628
TasI AATT 8 cut(s) 8, 22, 246, 406, 892, 1260, 1275, 1631
TatI WGTACW 1 cut(s) 1057
TauI GCSGC 3 cut(s) 109, 152, 286
TfiI GAWTC 1 cut(s) 1199
Tru1I TTAA 3 cut(s) 221, 405, 1607
Tru9I TTAA 3 cut(s) 221, 405, 1607
TscAI CASTG 9 cut(s) 534, 664, 850, 949, 1100, 1298, 1384, 1390, 1425
TseFI GTSAC 1 cut(s) 674
TseI GCWGC 9 cut(s) 259, 475, 478, 487, 577, 605, 608, 796, 816
Tsp45I GTSAC 1 cut(s) 674
TspDTI ATGAA 1 cut(s) 127
TspGWI ACGGA 2 cut(s) 462, 1231
TspRI CASTG 9 cut(s) 534, 664, 850, 949, 1100, 1298, 1384, 1390, 1425
Tth111I GACNNNGTC 1 cut(s) 238
Van91I CCANNNNNTGG 1 cut(s) 1379
Vha464I CTTAAG 1 cut(s) 1606
VpaK11BI GGWCC 2 cut(s) 394, 1288
XapI RAATTY 3 cut(s) 246, 892, 1275
XbaI TCTAGA 2 cut(s) 544, 1048
XmiI GTMKAC 1 cut(s) 584
XspI CTAG 7 cut(s) 132, 540, 545, 789, 996, 1049, 1514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.