pycom111g00630

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0003475
Physical Location & Seq
Forward (+)
421117 .. 425860
4744 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3678 bp
ATGGCTCGAGGGCAGGTGGAAAAAGTAGGATTTGCTGCTGGACGAGGGAGGTCTAACACTAATGGAAGCCTACAGATTGGCAAACTTGTACTAGGAAAATCTGGCCGTTTTGCCAATATGTATTGCTATCCCAGAGGAAAGCTCCTTGATATTTATCGCAAGCAAAAGAAGGATTCGACTTTTGATATGCCTGATGGGATGGAGCATGTATCACCAATAACCCAAGTGAGCTCGATTGAGCCATTGGCTTTTTTTGCCCCTGATGCAGAGGAAGAGGCTGGTCTGGGTGACATATATAAGGGAAGAATTACTAGCAGTGAAGTGGTATACAACTCGTCTGGGGACATGAACGTATTAGATGAAGATGCTAAAGGATCTAGCAATGTCAGTTTAAGCAAGGAAGAAGGGGAAAATGTCCAATCTGCTGAAAAGGTCAATTTAAACAATTCTTTTCCGGTTACTGGTGCTGAGTCACCTATTTGTGGCTCACAAACACATATTTTAAAAGAATGTGTTGCTACTGAAGGTCAGAAAGTTCTGACGGTCACAGCAGTGGCTGATAGGGAGATTGATCCCAGTAATGATGACGCTGACTTAAAAAGTTCAAGATGCCAGGAGCAAACCGCAAGCATTGATGATCACTATTTGAAGAGCAATGAGGACGCCTATCTCGTGGAAAGAATTGTCACACCTGAGGATATGAGTTTGTGCTACCTCGATCCTCAAGGGAATATCCAAGGACCTTTCCTGGGGATCGATATAATTGCATGGTTTGAGCAAGGATTTTTCGGAATTGACTTGCCAGTTCGTTCATTTGATGCGCCCAATGGATCTCTTTTTCAAGAACTTGGTGAAGTGATGCCACATCTGAAAACTAAATTTGGGCCTGTCTCTAATTCTAGTTTGCCTACTCAATTAGAGCCATCGGATGCTGTTGGAGGCAGCTTGGGAGAGAGAACATCTGCTCCTAACTATGATGGCGGGTCTGCTATTCTAAACAATCATGGCATTCAGAATGTTGTAGCAGAAGATGAGGAAATTGTATTTCCTGGAAGGCCTAAAAGCAGCAGTGATTACCTATCGAGGTCTTCAGCTGACATTCATGGCTCATTTGCAAATCCTCCGAGCCTTCATTCCCTTGCAAATGAAGTTTCAAAAACTAGTGTGCCTGATCAGCAGGACGATAAGTTGCATCCTTTTGGCCTGTTGATGTCAGAGCTCAGAGGCAACTCACGTTTAAGGCCTGCTCAATCATCTCATGCGTCTTTCGGCATTGATGATCAGGATCATTTTAGAGATGCCTTCTTTGAGGAAGGCGCCATTATTGCTAATCAGAATTCCCTTGGTGCCATGATTGATCAACCTTGCTTTGTGGAGACACGGCCTGATAATTATATAAAAAATAACACTAATGTTAGTATGGGTTCTATAGATCCTCACCATTTATCTCACATGGGAGAACAAAACAGTGGCATTGACCCGGCAGAGCATATAATGTCACAGCAATTACTAAAAGAACGGTTTCAGCAGCTGAACCATCTTTCTCCACTTACTGTTGCACATAGTGCAGGAACAGGAGTGGACCGGTTCCCTGGATTTGTTTTTTCTGACAGCAAGAACCCTAATATCCAGCCGTCATTCCATCATCCAGTGGCAGATATGGAACATATTTTTGAAATGCAGCGTCAGCAGCAGCGTCAGTTGGAGCTTCGTCAACAGCAACACCAATTGGAGCTTCAGCAACAGCAGCGTCAGTTGGAGATTCAGGAGCACCAGCGTCAGTTGGAGCTGCAGCGCCAGCAGCAGTTGGAGCTGCAGCGCCAGCAGCAGTTGGAGCTGCAGCGCCAGCAGCAGTTGGAGCTGCAGCGCCAGCAGCAGTTGGAGCTGCAGCGCCAGCAGCAGTTGGAGCTGCATCACCAACGTCAATTGGAGCTTCAGCAGCAACAGCGTCAGCTGGAGCTTCAGCAACAGCGTCACTTGCAGGAGCAGCTTCACCAACACCAAATGAAATTGCAGCAACAACAGAGATCTCAAGCTCAACATGTGCTCCTTGAACAGTTTTTGCATCAACAAATATCGGATTCTGGTTATGGGCAGTGGAAGGCGGATGCTACAAGAGGCAACCCTTTCGATCAATTACATTCAAGGAAGCACCTTCTAGATGACTTGCACCAGATGTCCCATTCCTCAAGACACGATCCATTGTTAGAGCATATCATCCAAGAAAATATAGGACGCTCAGGACAGACTGATTTCTTTGACCTCATATCTCAGGCCAAGCAAGGGAATATGCACATTTCTGATCTGCAGGCACAGCAGTTATCCGCAGCATTGAGAGAGCAACTGCAGATGGAAGGGGAAAGGCGTGTCGGTGGGCCTTGGTTCGTAGATGAAGCAAGCCAAGTTGTCAGGAATCCTGTTGGTCATCACCAGGCTCAGATGGTAGGATTTAGCTCTTCAGAAAATTACCAGCAACAGCAGAGTTTTCCCACCCATGAACAGCAACTAAGCCATCTCAATTGGAATCATGCCACACGGGAGCAACCTCAGGGAGGGTTCTACGAACCAAACTCTCTGGAATTTGAGAGGTCATCTGTTCCTGCTGGTTCCCATGCAATGAACTTGGATATTCTGAGTGCTCGCGGGCTGGATGAGTTTTATTCTTCTCATCCAGTTGGTTTGGAGCACTTGCCCTTTGGGAACAATGGACATCTGGAAGATAGCTCCATTGAATCACTACATTGTCATGCTGAGCAAACCCGCGACTCAGAAGCTACCATGGCTTTTGCTGATTCAGATATATGGGCATCATCTGATGGTGATAAAGAACACTCAAAACAATTTTTGATGGACCTTCACCAAAAATTCAGTCAATCTATACAGTTATCAGAAGTTGATTATCAGCATCACCTATCGTCTTCCGGAAGCCGGGGAGAATCTGTACATCATCCATTTAATCTTCTTCCTGATCAACCAGTTGGTATGAACAACTCCTTCACAGAAGGGCCTTTTAGATCTAATTCCAGAGCATTTGTGGAAGATTTATCAGAGTTGGAGGGAAATAATTGGAAGAAACAGGGAGTGAAAAGCTATGAAGGCAACATGGGAGAACAAGAAGAAACATCAAGGGATCTTCGTAGTAATGCCCATAGCAGGCAGAGTTCACTTAGCAGTGCTGGTGGAGGTGGGAGTTTTTACAGCTCTGAGACAGGGGTAGATAAACCACTTGGAGAAGAGGTTTCTAATGGGAGACTGCCTTCTACTGTAACCAAAGGGTATGACAATGCTTTGAATAGACGCCGAGTTTTATCTTCCCAGGACGTTTTGTCTGAAGCAGCAGTTCCTTTACCTGTCATACAAAGAAATCCAGCTGCAACCCAGGTCTATGAAACCCAAGCATGTAGCAAGAATGATGCGGAATTTAGGAGGACTTCATCTTACAGCGACGCATCTGTGTCTGAGACATCATTCATAGACATGCTCAAGAAACCAGTCATTTCAGAGGCTGATGCAGCCAACAGAGCAGCTGCACTGGAGCCGTCTGATGGAGGTGGCCAAGCGGGGCGGACTGGCAAGAAGAAAGGGAAGAAAGGAAGACAGATTGATCCAGCTCTCCTCGGCTTTAAGGTTACCAGCAACCGGATATTGATGGGTGAGATCCATCGCCTTAACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1226

Amino Acids

136.79

Weight (kDa)

5.38

Isoelectric Point (pI)

56.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 238 - 271 2.1e-09 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 4
Acc36I ACCTGC 1 cut(s) 4
AccB1I GGYRCC 2 cut(s) 1316, 1346
AccI GTMKAC 1 cut(s) 327
AccII CGCG 2 cut(s) 2643, 2763
AccIII TCCGGA 1 cut(s) 2921
AciI CCGC 9 cut(s) 624, 981, 2105, 2325, 2643, 2761, 3416, 3560, 3565
AcoI YGGCCR 2 cut(s) 103, 3553
AcsI RAATTY 5 cut(s) 878, 1336, 2579, 2864, 3419
AcuI CTGAAG 7 cut(s) 543, 1074, 1721, 1919, 1946, 2442, 3351
AcyI GRCGYC 3 cut(s) 663, 1317, 3298
AdeI CACNNNGTG 1 cut(s) 1565
AfaI GTAC 2 cut(s) 90, 2943
AfiI CCNNNNNNNGG 9 cut(s) 461, 482, 749, 2283, 2552, 2928, 3153, 3545, 3639
AflIII ACRYGT 1 cut(s) 2041
AgeI ACCGGT 1 cut(s) 1584
AgsI TTSAA 9 cut(s) 606, 649, 842, 1155, 1676, 2054, 2145, 2732, 3292
AhdI GACNNNNNGTC 1 cut(s) 3325
AhlI ACTAGT 1 cut(s) 1160
AjnI CCWGG 7 cut(s) 612, 747, 1048, 1591, 2430, 3315, 3378
AjuI GAANNNNNNNTTGG 2 cut(s) 1719, 1751
AleI CACNNNNGTG 1 cut(s) 551
AloI GAACNNNNNNTCC 2 cut(s) 949, 981
Alw21I GWGCWC 6 cut(s) 233, 1221, 1773, 2049, 2641, 2688
Alw26I GTCTC 5 cut(s) 895, 1370, 3200, 3244, 3455
AlwNI CAGNNNCTG 3 cut(s) 557, 1531, 3506
Ama87I CYCGRG 1 cut(s) 6
Aor13HI TCCGGA 1 cut(s) 2921
ApoI RAATTY 5 cut(s) 878, 1336, 2579, 2864, 3419
ArsI GACNNNNNNTTYG 2 cut(s) 2854, 2886
AsiGI ACCGGT 1 cut(s) 1584
Asp700I GAANNNNTTC 1 cut(s) 1521
AspLEI GCGC 7 cut(s) 823, 1319, 1797, 1821, 1845, 1869, 1893
AspS9I GGNCC 6 cut(s) 740, 884, 1582, 2375, 2851, 3005
AsuC2I CCSGG 2 cut(s) 1481, 2930
AvaI CYCGRG 1 cut(s) 6
AvaII GGWCC 3 cut(s) 740, 1582, 2851
AxyI CCTNAGG 2 cut(s) 693, 2547
BalI TGGCCA 1 cut(s) 3555
BanI GGYRCC 2 cut(s) 1316, 1346
BanII GRGCYC 2 cut(s) 233, 1221
BauI CACGAG 1 cut(s) 671
BbsI GAAGAC 3 cut(s) 1080, 2910, 3601
Bbv12I GWGCWC 6 cut(s) 233, 1221, 1773, 2049, 2641, 2688
BceAI ACGGC 4 cut(s) 90, 1397, 1618, 3523
BcgI CGANNNNNNTGC 4 cut(s) 746, 780, 2110, 2144
BciT130I CCWGG 7 cut(s) 614, 749, 1050, 1593, 2432, 3317, 3380
BclI TGATCA 5 cut(s) 637, 1171, 1279, 1357, 2968
BcnI CCSGG 2 cut(s) 1481, 2930
BcoDI GTCTC 5 cut(s) 895, 1370, 3200, 3244, 3455
BcuI ACTAGT 1 cut(s) 1160
BfaI CTAG 6 cut(s) 92, 312, 378, 900, 1161, 2159
BfmI CTRYAG 9 cut(s) 71, 1428, 1790, 1814, 1838, 1862, 1886, 2306, 2345
BfoI RGCGCY 6 cut(s) 1320, 1798, 1822, 1846, 1870, 1894
BfuAI ACCTGC 1 cut(s) 4
BglII AGATCT 2 cut(s) 2027, 3014
BlpI GCTNAGC 1 cut(s) 2751
Bme1390I CCNGG 9 cut(s) 614, 749, 1050, 1481, 1593, 2432, 2930, 3317, 3380
Bme18I GGWCC 3 cut(s) 740, 1582, 2851
BmeRI GACNNNNNGTC 1 cut(s) 3325
BmeT110I CYCGRG 1 cut(s) 6
BmgT120I GGNCC 6 cut(s) 740, 884, 1582, 2375, 2851, 3005
BmiI GGNNCC 5 cut(s) 1318, 1348, 1589, 2608, 3537
BmrFI CCNGG 9 cut(s) 614, 749, 1050, 1481, 1593, 2432, 2930, 3317, 3380
BmrI ACTGGG 1 cut(s) 570
BmuI ACTGGG 1 cut(s) 570
BpiI GAAGAC 3 cut(s) 1080, 2910, 3601
BplI GAGNNNNNCTC 2 cut(s) 126, 158
BpmI CTGGAG 2 cut(s) 1976, 3554
Bpu10I CCTNAGC 1 cut(s) 2239
Bpu1102I GCTNAGC 1 cut(s) 2751
BpuEI CTTGAG 4 cut(s) 708, 2016, 2173, 3467
BpuMI CCSGG 2 cut(s) 1481, 2930
Bsa29I ATCGAT 1 cut(s) 756
BsaBI GATNNNNATC 2 cut(s) 153, 1284
BsaHI GRCGYC 3 cut(s) 663, 1317, 3298
BsaWI WCCGGW 4 cut(s) 454, 1584, 2921, 3639
BsaXI ACNNNNNCTCC 8 cut(s) 949, 979, 2031, 2061, 2925, 2955, 3222, 3252
Bsc4I CCNNNNNNNGG 9 cut(s) 461, 482, 749, 2283, 2552, 2928, 3153, 3545, 3639
Bse118I RCCGGY 1 cut(s) 1584
Bse1I ACTGG 9 cut(s) 466, 576, 803, 1649, 2672, 2975, 3491, 3537, 3574
Bse21I CCTNAGG 2 cut(s) 693, 2547
Bse3DI GCAATG 3 cut(s) 388, 661, 2622
Bse8I GATNNNNATC 2 cut(s) 153, 1284
BseAI TCCGGA 1 cut(s) 2921
BseBI CCWGG 7 cut(s) 614, 749, 1050, 1593, 2432, 3317, 3380
BseCI ATCGAT 1 cut(s) 756
BseGI GGATG 9 cut(s) 204, 934, 1192, 1645, 2113, 2217, 2656, 2668, 2947
BseJI GATNNNNATC 2 cut(s) 153, 1284
BseLI CCNNNNNNNGG 9 cut(s) 461, 482, 749, 2283, 2552, 2928, 3153, 3545, 3639
BseMI GCAATG 3 cut(s) 388, 661, 2622
BseNI ACTGG 9 cut(s) 466, 576, 803, 1649, 2672, 2975, 3491, 3537, 3574
BseRI GAGGAG 1 cut(s) 3605
BsgI GTGCAG 2 cut(s) 1587, 3513
Bsh1236I CGCG 2 cut(s) 2643, 2763
BshNI GGYRCC 2 cut(s) 1316, 1346
BshTI ACCGGT 1 cut(s) 1584
BshVI ATCGAT 1 cut(s) 756
BsiHKAI GWGCWC 6 cut(s) 233, 1221, 1773, 2049, 2641, 2688
BsiHKCI CYCGRG 1 cut(s) 6
BsiSI CCGG 6 cut(s) 455, 1481, 1585, 2922, 2929, 3640
BslFI GGGAC 2 cut(s) 356, 2164
BslI CCNNNNNNNGG 9 cut(s) 461, 482, 749, 2283, 2552, 2928, 3153, 3545, 3639
BsmAI GTCTC 5 cut(s) 895, 1370, 3200, 3244, 3455
BsmFI GGGAC 2 cut(s) 356, 2164
BsmI GAATGC 1 cut(s) 1008
BsoBI CYCGRG 1 cut(s) 6
Bsp1286I GDGCHC 6 cut(s) 233, 1221, 1773, 2049, 2641, 2688
Bsp13I TCCGGA 1 cut(s) 2921
Bsp1407I TGTACA 1 cut(s) 2941
Bsp1720I GCTNAGC 1 cut(s) 2751
Bsp19I CCATGG 1 cut(s) 2778
BspACI CCGC 9 cut(s) 624, 981, 2105, 2325, 2643, 2761, 3416, 3560, 3565
BspDI ATCGAT 1 cut(s) 756
BspEI TCCGGA 1 cut(s) 2921
BspFNI CGCG 2 cut(s) 2643, 2763
BspLI GGNNCC 5 cut(s) 1318, 1348, 1589, 2608, 3537
BspMAI CTGCAG 7 cut(s) 1794, 1818, 1842, 1866, 1890, 2310, 2349
BspMI ACCTGC 1 cut(s) 4
BspQI GCTCTTC 2 cut(s) 644, 2461
BspT107I GGYRCC 2 cut(s) 1316, 1346
BsrDI GCAATG 3 cut(s) 388, 661, 2622
BsrFI RCCGGY 1 cut(s) 1584
BsrGI TGTACA 1 cut(s) 2941
BsrI ACTGG 9 cut(s) 466, 576, 803, 1649, 2672, 2975, 3491, 3537, 3574
BssAI RCCGGY 1 cut(s) 1584
BssNAI GTATAC 1 cut(s) 328
BssNI GRCGYC 3 cut(s) 663, 1317, 3298
BssSI CACGAG 1 cut(s) 671
BssT1I CCWWGG 4 cut(s) 736, 1342, 2378, 2778
Bst1107I GTATAC 1 cut(s) 328
Bst2BI CACGAG 1 cut(s) 671
Bst2UI CCWGG 7 cut(s) 614, 749, 1050, 1593, 2432, 3317, 3380
Bst4CI ACNGT 7 cut(s) 544, 1469, 1521, 1555, 2058, 2883, 3265
Bst6I CTCTTC 4 cut(s) 267, 644, 2461, 3228
BstACI GRCGYC 3 cut(s) 663, 1317, 3298
BstAPI GCANNNNNTGC 1 cut(s) 1565
BstAUI TGTACA 1 cut(s) 2941
BstDSI CCRYGG 1 cut(s) 2778
BstEII GGTNACC 1 cut(s) 3628
BstENI CCTNNNNNAGG 1 cut(s) 2550
BstF5I GGATG 9 cut(s) 204, 934, 1192, 1645, 2113, 2217, 2656, 2668, 2947
BstFNI CGCG 2 cut(s) 2643, 2763
BstH2I RGCGCY 6 cut(s) 1320, 1798, 1822, 1846, 1870, 1894
BstHHI GCGC 7 cut(s) 823, 1319, 1797, 1821, 1845, 1869, 1893
BstMAI GTCTC 5 cut(s) 895, 1370, 3200, 3244, 3455
BstNI CCWGG 7 cut(s) 614, 749, 1050, 1593, 2432, 3317, 3380
BstNSI RCATGY 4 cut(s) 209, 2045, 3402, 3481
BstPI GGTNACC 1 cut(s) 3628
BstSCI CCNGG 9 cut(s) 612, 747, 1048, 1479, 1591, 2430, 2928, 3315, 3378
BstSFI CTRYAG 9 cut(s) 71, 1428, 1790, 1814, 1838, 1862, 1886, 2306, 2345
BstUI CGCG 2 cut(s) 2643, 2763
BstV2I GAAGAC 3 cut(s) 1080, 2910, 3601
BstX2I RGATCY 7 cut(s) 374, 830, 1432, 2027, 3014, 3130, 3657
BstYI RGATCY 7 cut(s) 374, 830, 1432, 2027, 3014, 3130, 3657
BstZ17I GTATAC 1 cut(s) 328
Bsu15I ATCGAT 1 cut(s) 756
Bsu36I CCTNAGG 2 cut(s) 693, 2547
BsuTUI ATCGAT 1 cut(s) 756
BtgI CCRYGG 1 cut(s) 2778
BtgZI GCGATG 1 cut(s) 3647
BtsCI GGATG 9 cut(s) 204, 934, 1192, 1645, 2113, 2217, 2656, 2668, 2947
BtsI GCAGTG 5 cut(s) 322, 558, 1075, 2102, 3178
BtsIMutI CAGTG 8 cut(s) 322, 558, 1075, 1474, 1656, 2102, 3178, 3530
BveI ACCTGC 1 cut(s) 4
CaiI CAGNNNCTG 3 cut(s) 557, 1531, 3506
CfoI GCGC 7 cut(s) 823, 1319, 1797, 1821, 1845, 1869, 1893
Cfr10I RCCGGY 1 cut(s) 1584
Cfr13I GGNCC 6 cut(s) 740, 884, 1582, 2375, 2851, 3005
ClaI ATCGAT 1 cut(s) 756
Csp6I GTAC 2 cut(s) 89, 2942
CspAI ACCGGT 1 cut(s) 1584
CviQI GTAC 2 cut(s) 89, 2942
DinI GGCGCC 1 cut(s) 1318
DraI TTTAAA 2 cut(s) 441, 504
DraIII CACNNNGTG 1 cut(s) 1565
DriI GACNNNNNGTC 1 cut(s) 3325
EaeI YGGCCR 2 cut(s) 103, 3553
Eam1104I CTCTTC 4 cut(s) 267, 644, 2461, 3228
Eam1105I GACNNNNNGTC 1 cut(s) 3325
EarI CTCTTC 4 cut(s) 267, 644, 2461, 3228
EciI GGCGGA 2 cut(s) 2120, 3580
Ecl136II GAGCTC 2 cut(s) 231, 1219
Eco130I CCWWGG 4 cut(s) 736, 1342, 2378, 2778
Eco147I AGGCCT 2 cut(s) 1057, 1243
Eco24I GRGCYC 2 cut(s) 233, 1221
Eco47I GGWCC 3 cut(s) 740, 1582, 2851
Eco53kI GAGCTC 2 cut(s) 231, 1219
Eco57I CTGAAG 7 cut(s) 543, 1074, 1721, 1919, 1946, 2442, 3351
Eco81I CCTNAGG 2 cut(s) 693, 2547
Eco88I CYCGRG 1 cut(s) 6
Eco91I GGTNACC 1 cut(s) 3628
EcoICRI GAGCTC 2 cut(s) 231, 1219
EcoNI CCTNNNNNAGG 1 cut(s) 2550
EcoO109I RGGNCCY 2 cut(s) 740, 3005
EcoO65I GGTNACC 1 cut(s) 3628
EcoRI GAATTC 1 cut(s) 1336
EcoRII CCWGG 7 cut(s) 612, 747, 1048, 1591, 2430, 3315, 3378
EcoT14I CCWWGG 4 cut(s) 736, 1342, 2378, 2778
EcoT38I GRGCYC 2 cut(s) 233, 1221
EgeI GGCGCC 1 cut(s) 1318
EheI GGCGCC 1 cut(s) 1318
ErhI CCWWGG 4 cut(s) 736, 1342, 2378, 2778
FaqI GGGAC 2 cut(s) 356, 2164
FauI CCCGC 4 cut(s) 974, 2636, 2768, 3553
FbaI TGATCA 5 cut(s) 637, 1171, 1279, 1357, 2968
FblI GTMKAC 1 cut(s) 327
FokI GGATG 9 cut(s) 211, 941, 1179, 1632, 2120, 2204, 2655, 2663, 2934
FriOI GRGCYC 2 cut(s) 233, 1221
FspBI CTAG 6 cut(s) 92, 312, 378, 900, 1161, 2159
GlaI GCGC 7 cut(s) 822, 1318, 1796, 1820, 1844, 1868, 1892
GsuI CTGGAG 2 cut(s) 1976, 3554
HaeII RGCGCY 6 cut(s) 1320, 1798, 1822, 1846, 1870, 1894
HapII CCGG 6 cut(s) 455, 1481, 1585, 2922, 2929, 3640
HhaI GCGC 7 cut(s) 823, 1319, 1797, 1821, 1845, 1869, 1893
Hin1I GRCGYC 3 cut(s) 663, 1317, 3298
Hin6I GCGC 7 cut(s) 821, 1317, 1795, 1819, 1843, 1867, 1891
HinP1I GCGC 7 cut(s) 821, 1317, 1795, 1819, 1843, 1867, 1891
HincII GTYRAC 1 cut(s) 1715
HindII GTYRAC 1 cut(s) 1715
HpaII CCGG 6 cut(s) 455, 1481, 1585, 2922, 2929, 3640
Hpy166II GTNNAC 4 cut(s) 328, 1582, 1715, 3164
Hpy8I GTNNAC 4 cut(s) 328, 1582, 1715, 3164
Hpy99I CGWCG 1 cut(s) 3449
HpyCH4III ACNGT 7 cut(s) 544, 1469, 1521, 1555, 2058, 2883, 3265
HpyCH4IV ACGT 4 cut(s) 351, 1234, 1921, 3321
HpySE526I ACGT 4 cut(s) 351, 1234, 1921, 3321
Hsp92I GRCGYC 3 cut(s) 663, 1317, 3298
HspAI GCGC 7 cut(s) 821, 1317, 1795, 1819, 1843, 1867, 1891
KasI GGCGCC 1 cut(s) 1316
Kpn2I TCCGGA 1 cut(s) 2921
Ksp22I TGATCA 5 cut(s) 637, 1171, 1279, 1357, 2968
LguI GCTCTTC 2 cut(s) 644, 2461
MaeI CTAG 6 cut(s) 92, 312, 378, 900, 1161, 2159
MaeII ACGT 4 cut(s) 351, 1234, 1921, 3321
MaeIII GTNAC 9 cut(s) 287, 457, 471, 544, 685, 1497, 1973, 3265, 3628
MfeI CAATTG 3 cut(s) 1727, 1925, 2518
MflI RGATCY 7 cut(s) 374, 830, 1432, 2027, 3014, 3130, 3657
MhlI GDGCHC 6 cut(s) 233, 1221, 1773, 2049, 2641, 2688
MlsI TGGCCA 1 cut(s) 3555
MluNI TGGCCA 1 cut(s) 3555
Mly113I GGCGCC 1 cut(s) 1317
MlyI GAGTC 2 cut(s) 479, 2759
Mox20I TGGCCA 1 cut(s) 3555
MroI TCCGGA 1 cut(s) 2921
MroXI GAANNNNTTC 1 cut(s) 1521
MscI TGGCCA 1 cut(s) 3555
MseI TTAA 8 cut(s) 392, 440, 503, 596, 1238, 2955, 3624, 3669
MslI CAYNNNNRTG 3 cut(s) 551, 2745, 3476
Msp20I TGGCCA 1 cut(s) 3555
MspA1I CMGCKG 5 cut(s) 1094, 1531, 1954, 3371, 3527
MspI CCGG 6 cut(s) 455, 1481, 1585, 2922, 2929, 3640
MspR9I CCNGG 9 cut(s) 614, 749, 1050, 1481, 1593, 2432, 2930, 3317, 3380
MunI CAATTG 3 cut(s) 1727, 1925, 2518
Mva1269I GAATGC 1 cut(s) 1008
MvaI CCWGG 7 cut(s) 614, 749, 1050, 1593, 2432, 3317, 3380
MvnI CGCG 2 cut(s) 2643, 2763
NarI GGCGCC 1 cut(s) 1317
NciI CCSGG 2 cut(s) 1481, 2930
NcoI CCATGG 1 cut(s) 2778
NlaIV GGNNCC 5 cut(s) 1318, 1348, 1589, 2608, 3537
NmeAIII GCCGAG 2 cut(s) 3326, 3597
NmuCI GTSAC 6 cut(s) 287, 471, 544, 685, 1497, 1973
NspI RCATGY 4 cut(s) 209, 2045, 3402, 3481
OliI CACNNNNGTG 1 cut(s) 551
PaeR7I CTCGAG 1 cut(s) 6
PaqCI CACCTGC 1 cut(s) 4
PceI AGGCCT 2 cut(s) 1057, 1243
PciI ACATGT 1 cut(s) 2041
PciSI GCTCTTC 2 cut(s) 644, 2461
PcsI WCGNNNNNNNCGW 1 cut(s) 669
PctI GAATGC 1 cut(s) 1008
PdmI GAANNNNTTC 1 cut(s) 1521
PfeI GAWTC 8 cut(s) 173, 1762, 2081, 2413, 2524, 2732, 2792, 2936
PfoI TCCNGGA 1 cut(s) 1048
PinAI ACCGGT 1 cut(s) 1584
PleI GAGTC 2 cut(s) 478, 2759
PluTI GGCGCC 1 cut(s) 1320
PpsI GAGTC 2 cut(s) 478, 2759
PpuMI RGGWCCY 1 cut(s) 740
PscI ACATGT 1 cut(s) 2041
Psp124BI GAGCTC 2 cut(s) 233, 1221
Psp5II RGGWCCY 1 cut(s) 740
Psp6I CCWGG 7 cut(s) 612, 747, 1048, 1591, 2430, 3315, 3378
PspEI GGTNACC 1 cut(s) 3628
PspGI CCWGG 7 cut(s) 612, 747, 1048, 1591, 2430, 3315, 3378
PspN4I GGNNCC 5 cut(s) 1318, 1348, 1589, 2608, 3537
PspPI GGNCC 6 cut(s) 740, 884, 1582, 2375, 2851, 3005
PspPPI RGGWCCY 1 cut(s) 740
PspXI VCTCGAGB 1 cut(s) 6
PstI CTGCAG 7 cut(s) 1794, 1818, 1842, 1866, 1890, 2310, 2349
PstNI CAGNNNCTG 3 cut(s) 557, 1531, 3506
PsuI RGATCY 7 cut(s) 374, 830, 1432, 2027, 3014, 3130, 3657
PvuII CAGCTG 5 cut(s) 1094, 1531, 1954, 3371, 3527
RsaI GTAC 2 cut(s) 90, 2943
RsaNI GTAC 2 cut(s) 89, 2942
RseI CAYNNNNRTG 3 cut(s) 551, 2745, 3476
SacI GAGCTC 2 cut(s) 233, 1221
SapI GCTCTTC 2 cut(s) 644, 2461
SaqAI TTAA 8 cut(s) 392, 440, 503, 596, 1238, 2955, 3624, 3669
Sau96I GGNCC 6 cut(s) 740, 884, 1582, 2375, 2851, 3005
SchI GAGTC 2 cut(s) 479, 2759
ScrFI CCNGG 9 cut(s) 614, 749, 1050, 1481, 1593, 2432, 2930, 3317, 3380
SduI GDGCHC 6 cut(s) 233, 1221, 1773, 2049, 2641, 2688
SfcI CTRYAG 9 cut(s) 71, 1428, 1790, 1814, 1838, 1862, 1886, 2306, 2345
SfoI GGCGCC 1 cut(s) 1318
Sfr274I CTCGAG 1 cut(s) 6
SinI GGWCC 3 cut(s) 740, 1582, 2851
SlaI CTCGAG 1 cut(s) 6
SmiMI CAYNNNNRTG 3 cut(s) 551, 2745, 3476
SmlI CTYRAG 5 cut(s) 6, 723, 2031, 2188, 3482
SmoI CTYRAG 5 cut(s) 6, 723, 2031, 2188, 3482
SpeI ACTAGT 1 cut(s) 1160
SseBI AGGCCT 2 cut(s) 1057, 1243
SsiI CCGC 9 cut(s) 624, 981, 2105, 2325, 2643, 2761, 3416, 3560, 3565
SspDI GGCGCC 1 cut(s) 1316
SspMI CTAG 6 cut(s) 92, 312, 378, 900, 1161, 2159
SstI GAGCTC 2 cut(s) 233, 1221
StuI AGGCCT 2 cut(s) 1057, 1243
StyD4I CCNGG 9 cut(s) 612, 747, 1048, 1479, 1591, 2430, 2928, 3315, 3378
StyI CCWWGG 4 cut(s) 736, 1342, 2378, 2778
TaaI ACNGT 7 cut(s) 544, 1469, 1521, 1555, 2058, 2883, 3265
TaiI ACGT 4 cut(s) 354, 1237, 1924, 3324
TaqI TCGA 7 cut(s) 7, 176, 233, 717, 756, 1082, 2130
TatI WGTACW 2 cut(s) 88, 2941
TfiI GAWTC 8 cut(s) 173, 1762, 2081, 2413, 2524, 2732, 2792, 2936
Tru1I TTAA 8 cut(s) 392, 440, 503, 596, 1238, 2955, 3624, 3669
Tru9I TTAA 8 cut(s) 392, 440, 503, 596, 1238, 2955, 3624, 3669
TscAI CASTG 8 cut(s) 322, 558, 1075, 1474, 1656, 2102, 3178, 3537
TseFI GTSAC 6 cut(s) 287, 471, 544, 685, 1497, 1973
Tsp45I GTSAC 6 cut(s) 287, 471, 544, 685, 1497, 1973
TspRI CASTG 8 cut(s) 322, 558, 1075, 1474, 1656, 2102, 3178, 3537
VpaK11BI GGWCC 3 cut(s) 740, 1582, 2851
XagI CCTNNNNNAGG 1 cut(s) 2550
XapI RAATTY 5 cut(s) 878, 1336, 2579, 2864, 3419
XbaI TCTAGA 1 cut(s) 2158
XceI RCATGY 4 cut(s) 209, 2045, 3402, 3481
XcmI CCANNNNNNNNNTGG 1 cut(s) 3031
XhoI CTCGAG 1 cut(s) 6
XmiI GTMKAC 1 cut(s) 327
XmnI GAANNNNTTC 1 cut(s) 1521
XspI CTAG 6 cut(s) 92, 312, 378, 900, 1161, 2159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.