Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

SuperScaffold_111

Orthogroup ID

OG0009542

Physical Location & Seq

Genomic Coordinates Forward (+)

3520029 .. 3524358

Gene Structure 4330 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3318 bp

ATGGAGGAAGTGTGCGAGGGCAAGGACTTCTCCTTCCCCAAACAAGAAGAAACCATCCTCAAACTCTGGTCCGAAATCAAAGCCTTCGAAACCCAATTGGCGCGCACGGAGGGCCAGCCGGAATACGTCTTCTACGACGGCCCTCCGTTTGCCACCGGCCTCCCGCACTATGGCCACATCTTGGCCGGAACCATCAAAGACATCATCACCCGATTCTTCTCCATGACCGGCCACCACGTCATCCGGCGATTCGGGTGGGACTGCCACGGCCTGCCTGTGGAGAACGAGATCGACCGGACTCACGGTATCAAGCGCCGGGACGACGTGCTGAATATGGGGATCGATAAGTACAACGAGGCCTGTAGAAGCATTGTGACGCGGTACGTCGGCGAGTGGGAGAAGGTTATCACCCGGACGGGGCGTTGGATTGATTTCCAGAACGATTACAAGACGATGGATTTGAAGTTTATGGAATCTGTGTGGTGGGTTTTTGCGCAATTGTTTGAAAAGGGGCTGGTTTATAAAGGATTCAAGGTCATGCCATACAGCACTGGTTGCAAGACACCACTCTCAAATTTTGAGGCTGGTCAAGACTATAGGGATGTGCCTGATCCCGAAATTATGGTGACTTTTCCAATTGTGGGAGATCTGGAAAATGCAAGTTTTGTGGCTTGGACAACTACACCATGGACCCTCCCTAGTAACTTAGCCCTCTGTGTCAATGCGAACTTTACTTATGTGAAGGTTCGCAACAACTACTCGGGGAAAGTTTATGTTGTTGCTGAATCTCGGTTGTCAGCTCTTCCTAGTGAGAAACCAAAAGAGAAAAGTACTGTAGTAGAAAATGGGTCCAAGTCTAAGACCAAGGGAGGAGCTGGGAAAAAGGAAACTGTCGACAGTTCATCATATCAAGTATTGCAGAAATTCTCAGGGGCTTCATTAGTGGGAAACAAGTATGTGCCACTGTTTGACTACTTTAAGGAGTTCTCTGATGTGGCATTTAGAGTTGTTGCGGACAACTATGTTACTGATGATAGTGGGACTGGTATTGTTCATTGTGCTCCTGCCTTTGGCGAGGATGATTATCGTGTTTGCCTTGAAAACCAAGTAATCAATAAGGGAGACAACTTGATTGTAGCGGTTGATGATGATGGCTGCTTTACAGAAAGAATCACTGATTTCAGCCGGTTCTATGTCAAGGATGCCGATAAAGATATTATTGAAGCAGTGAAGGCAAAAGGAAGGCTAGTGAAGTCCGGAACTTTTACTCATTCTTATCCCTTTTGCTGGAGATCTAAAACTCCACTTATCTACAGAGCTGTTCCAAGTTGGTTTATCCGGGTGGAGCAGTTGAAAGAAAAATTGCTAGAAAATAACACACAGACTTACTGGGTTCCTGACTTTGTCAAGGAAAAGCGCTTCCACAATTGGCTGGAAAATGCAAGAGATTGGGCCGTCAGTCGAAGTAGGTTTTGGGGAACTCCTCTCCCTGTGTGGGTTAGTGAGGATGGTGAGGAGATACTAGTCATCGATTCTATTGATAAGCTTGAAAAGCTTTCCGGTGTTAAGGTTTTTGATCTGCACCGGCACAATATCGATCATATCACTATTCCATCTGCTCGTGGTCCGCAACATGGTGTTCTTCGACGTATAGATGATGTGTTTGATTGCTGGTTTGAGAGTGGATCCATGCCTTATGCTTATATCCACTATCCTTTCGAGAATGTTGAGCTTTTTGAGAAAAATTTTCCTGGGCATTTCGTTGCGGAAGGGCTAGATCAGACTCGTGGGTGGTTCTACACTCTTATGGTGTTATCTACTGCGTTATTTGGGAAACCTGCTTTTAGAAACCTGATTTGCAATGGACTTGTCCTTGCTGAGGATGGAAAAAAAATGAGTAAAAGTTTGAAGAACTATCCTTCACCAATGGAGGTTATTAATGACTATGGTGCTGATGCACTACGATTATACCTCATCAACTCTCCTGTTGTGCGTGCGGAGACATTACGTTTCAAGAAGGAAGGAGTTTTTGGTGTGGTCAAGGATGTATTTCTTCCTTGGTACAATGCATACAGGTTCCTTGTTCAGAATGCAAAGAGACTTGAAGATGAAGGGTTTGGCACTTTCCTCCCTATTGATCTAGCCACTGTACAAACATCGTCTAATGTGCTTGACCAGTGGATCAACTCAGCCACACAGAGTCTAGTGCATTTTGTCCAACAAGAGATGAATGGTTATCGACTTTACACTGTGGTTCCATATCTTTTGAAGTTTCTTGACAACTTGACAAATATTTATGTGCGGTTCAATCGCAAGAGGCTAAAGGGTCGTACTGGTGAAGAAGATTGTAGGATGGCACTCTCAACTCTTTACAATGTCCTCTTAGTGTCCTGTAAAGTGATGGCACCATTAACGCCATTCTTCACTGAGGTGCTTTATCAAAATTTGCGGAAAATTTTGAATGAGTCAGAGGAAAGCATTCACTTTTGTAGTTTTCCTAAAGCAGAAGGAAAGAGGGATGAACGAATGGAGCAGAGTGTTAGAAGAATGATGACCGTGATTGATCTTGGCCGTAACATTCGTGAGCGTCACAATAAGCCTCTCAAGACACCCCTTAAGGAGATGGTTATAGTTCATCCTGATGCAGACTTTCTTGATGATATTGCTGCAAAGCTGAGAGAGTATGTGTTGGAGGAACTTAATGTACGATCTCTTGTCACGTGTAATGATACATTGGAGTATGCTTCGTTACGTGCAGAGCCTGATTTTAGTGTACTGGGTAAGCGGCTTGGAAAATCGATGGGGGTTGTTGCCAAAGAAGTCAAGGCCATGTCCCAGGAAAGCATTTTAGCATTTGAGAAAGCTGGAGAAGTTACTGTTTCTGGTCATTGTTTAAAGGTTGCTGATATTAAGGTGGTTAGGGATTTCAAACGTCCTAATGGAACGACAGAAAAGGAGATAGATGCAGCGGGAGATGGTGATGTTTTGGTGGTTTTGGATTTACGTCCAGATGAGTCCTTGTTCGAGGCTGGTGTTGCTCGCGAACTTGTTAGGAGAGTTCAACAGTTACGGAAGACAGCTGCCCTTAAACCTACTGACGTGGTGGAGGCCTACTTTGAATCTTTAGATCAAGATAAATCAGTCTCACGAAGGGTTTTACAATCCCAGGAAGAATACATTACAGATGCCATTGGTTTTCCATTGCGTCCTTCTCAAGTGATGCCATCAGATGCTGTCCTTGTCGCAGAGGAGAGTTCCCATGGGGTATCGGGCATGTCATTTGTTATCAGATTGGTTCGCCCGACCAGTTCCAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004822 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006428 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0017101 GO:0019538 GO:0019752 GO:0019899 GO:0032991 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0051020 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1

Protein Analysis

1106

Amino Acids

126.07

Weight (kDa)

6.18

Isoelectric Point (pI)

40.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	19 - 669	3.1e-156	tRNA synthetases class I (I, L, M and V)
Anticodon_1	PF08264	725 - 874	3.8e-27	Anticodon-binding domain of tRNA ligase
DUF5915	PF19302	894 - 1061	1.1e-23	Domain of unknown function (DUF5915)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009542)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G10320
fragaria_vesca	FvH4_4g11470
malus_domestica	MD04G1037200.v1.1 MD09G1065200.v1.1
prunus_persica	Prupe.1G186600_v2.0.a1 Prupe.1G186600_v2.0.a1
pyrus_communis	pycom04g03030 pycom111g05510
rosa_chinensis	RchiOBHm_Chr4g0408181
rosa_laevigata	RLG00000008666 RLG00000035346
rosa_multiflora	Rmu_ssc0000039.1_g000001
rosa_roxburghii	Rroxscaffold_1G00020510 Rroxscaffold_5G00352680
rosa_rugosa	Rorug04G0083900 Rorug04G0084000 Rorug05G0328300
rosa_samantha	Rh4AG148700 Rh4BG145500 Rh4CG155200 Rh4DG142000
rosa_wichuraiana	Rw4G012140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	522
Acc16I	TGCGCA	1 cut(s)	495
Acc36I	ACCTGC	1 cut(s)	1846
AccB1I	GGYRCC	1 cut(s)	2404
AccB7I	CCANNNNNTGG	1 cut(s)	181
AccI	GTMKAC	1 cut(s)	894
AccII	CGCG	3 cut(s)	103, 379, 3042
AccIII	TCCGGA	1 cut(s)	1256
AclWI	GGATC	5 cut(s)	347, 605, 1680, 1693, 2189
AcoI	YGGCCR	4 cut(s)	172, 183, 229, 2569
AcsI	RAATTY	5 cut(s)	574, 923, 1744, 2443, 2454
AcvI	CACGTG	1 cut(s)	2721
AfaI	GTAC	8 cut(s)	350, 383, 832, 2063, 2151, 2332, 2706, 2775
AfeI	AGCGCT	1 cut(s)	1418
AflII	CTTAAG	1 cut(s)	2615
AflIII	ACRYGT	1 cut(s)	2720
AhlI	ACTAGT	1 cut(s)	1522
AjiI	CACGTC	3 cut(s)	238, 325, 3100
AjnI	CCWGG	3 cut(s)	1750, 2835, 3165
AjuI	GAANNNNNNNTTGG	4 cut(s)	1456, 1488, 2013, 2045
AleI	CACNNNNGTG	1 cut(s)	2429
AluBI	AGCT	9 cut(s)	800, 875, 1319, 1546, 1555, 1732, 2674, 2864, 3080
AluI	AGCT	9 cut(s)	800, 875, 1319, 1546, 1555, 1732, 2674, 2864, 3080
Alw21I	GWGCWC	1 cut(s)	1063
Alw26I	GTCTC	4 cut(s)	1116, 1994, 2092, 3148
AlwI	GGATC	5 cut(s)	347, 605, 1680, 1693, 2189
AlwNI	CAGNNNCTG	2 cut(s)	2762, 3233
Ama87I	CYCGRG	1 cut(s)	760
Aor13HI	TCCGGA	1 cut(s)	1256
Aor51HI	AGCGCT	1 cut(s)	1418
ApeKI	GCWGC	4 cut(s)	1155, 2666, 2966, 3080
ApoI	RAATTY	5 cut(s)	574, 923, 1744, 2443, 2454
ArsI	GACNNNNNNTTYG	2 cut(s)	442, 474
AseI	ATTAAT	1 cut(s)	1938
Asp700I	GAANNNNTTC	1 cut(s)	2478
AspLEI	GCGC	5 cut(s)	103, 105, 315, 496, 1419
AspS9I	GGNCC	7 cut(s)	69, 112, 140, 690, 849, 1452, 1625
AsuC2I	CCSGG	3 cut(s)	317, 412, 1340
AsuHPI	GGTGA	7 cut(s)	199, 400, 637, 1523, 1914, 2348, 2990
AsuII	TTCGAA	1 cut(s)	87
AvaI	CYCGRG	1 cut(s)	760
AvaII	GGWCC	4 cut(s)	69, 690, 849, 1625
BalI	TGGCCA	1 cut(s)	174
BamHI	GGATCC	1 cut(s)	1685
BanI	GGYRCC	1 cut(s)	2404
BauI	CACGAG	2 cut(s)	1620, 1785
BbrPI	CACGTG	1 cut(s)	2721
BbsI	GAAGAC	2 cut(s)	121, 3080
Bbv12I	GWGCWC	1 cut(s)	1063
BbvCI	CCTCAGC	1 cut(s)	1878
BbvI	GCAGC	4 cut(s)	1142, 2653, 2978, 3067
BceAI	ACGGC	4 cut(s)	154, 283, 1439, 2556
BciT130I	CCWGG	3 cut(s)	1752, 2837, 3167
BcnI	CCSGG	3 cut(s)	317, 412, 1340
BcoDI	GTCTC	4 cut(s)	1116, 1994, 2092, 3148
BcuI	ACTAGT	1 cut(s)	1522
BfaI	CTAG	8 cut(s)	699, 807, 1247, 1367, 1523, 1775, 2141, 2204
BfmI	CTRYAG	4 cut(s)	361, 595, 834, 1312
BfoI	RGCGCY	2 cut(s)	316, 1420
BfrI	CTTAAG	1 cut(s)	2615
BfuAI	ACCTGC	1 cut(s)	1846
BglII	AGATCT	2 cut(s)	646, 1292
BisI	GCNGC	5 cut(s)	1156, 2667, 2786, 2967, 3081
BlsI	GCNGC	5 cut(s)	1157, 2668, 2787, 2968, 3082
BmcAI	AGTACT	1 cut(s)	832
Bme1390I	CCNGG	6 cut(s)	317, 412, 1340, 1752, 2837, 3167
Bme18I	GGWCC	4 cut(s)	69, 690, 849, 1625
BmeT110I	CYCGRG	1 cut(s)	760
BmgBI	CACGTC	3 cut(s)	238, 325, 3100
BmgT120I	GGNCC	7 cut(s)	69, 112, 140, 690, 849, 1452, 1625
BmiI	GGNNCC	8 cut(s)	190, 692, 850, 1395, 1687, 2078, 2256, 2406
BmrFI	CCNGG	6 cut(s)	317, 412, 1340, 1752, 2837, 3167
BmrI	ACTGGG	2 cut(s)	1399, 2786
BmsI	GCATC	7 cut(s)	1192, 1945, 2632, 2953, 3175, 3210, 3220
BmuI	ACTGGG	2 cut(s)	1399, 2786
BpiI	GAAGAC	2 cut(s)	121, 3080
BpmI	CTGGAG	2 cut(s)	1309, 2886
Bpu10I	CCTNAGC	1 cut(s)	1878
Bpu14I	TTCGAA	1 cut(s)	87
BpuEI	CTTGAG	2 cut(s)	2588, 3198
BpuMI	CCSGG	3 cut(s)	317, 412, 1340
Bsa29I	ATCGAT	4 cut(s)	342, 1530, 1596, 2798
BsaAI	YACGTR	2 cut(s)	2721, 2753
BsaBI	GATNNNNATC	1 cut(s)	1083
BsaJI	CCNNGG	8 cut(s)	265, 686, 864, 1751, 2057, 2835, 3165, 3259
BsaWI	WCCGGW	3 cut(s)	294, 1256, 1559
BsaXI	ACNNNNNCTCC	1 cut(s)	26
Bse118I	RCCGGY	4 cut(s)	155, 227, 1185, 1584
Bse1I	ACTGG	7 cut(s)	556, 1048, 1394, 2176, 2338, 2781, 3306
Bse3DI	GCAATG	2 cut(s)	1867, 3200
Bse8I	GATNNNNATC	1 cut(s)	1083
BseAI	TCCGGA	1 cut(s)	1256
BseBI	CCWGG	3 cut(s)	1752, 2837, 3167
BseCI	ATCGAT	4 cut(s)	342, 1530, 1596, 2798
BseDI	CCNNGG	8 cut(s)	265, 686, 864, 1751, 2057, 2835, 3165, 3259
BseJI	GATNNNNATC	1 cut(s)	1083
BseMI	GCAATG	2 cut(s)	1867, 3200
BseMII	CTCAG	5 cut(s)	942, 1869, 2202, 2418, 2666
BseNI	ACTGG	7 cut(s)	556, 1048, 1394, 2176, 2338, 2781, 3306
BsePI	GCGCGC	1 cut(s)	101
BseRI	GAGGAG	4 cut(s)	885, 1473, 1529, 3263
BseXI	GCAGC	4 cut(s)	1142, 2653, 2978, 3067
BseYI	CCCAGC	1 cut(s)	875
BsgI	GTGCAG	2 cut(s)	1565, 2775
Bsh1236I	CGCG	3 cut(s)	103, 379, 3042
Bsh1285I	CGRYCG	1 cut(s)	295
BshNI	GGYRCC	1 cut(s)	2404
BshVI	ATCGAT	4 cut(s)	342, 1530, 1596, 2798
BsiEI	CGRYCG	1 cut(s)	295
BsiHKAI	GWGCWC	1 cut(s)	1063
BsiHKCI	CYCGRG	1 cut(s)	760
BslFI	GGGAC	4 cut(s)	272, 332, 1054, 2818
BsmAI	GTCTC	4 cut(s)	1116, 1994, 2092, 3148
BsmFI	GGGAC	4 cut(s)	272, 332, 1054, 2818
BsmI	GAATGC	2 cut(s)	2095, 2478
BsoBI	CYCGRG	1 cut(s)	760
Bsp119I	TTCGAA	1 cut(s)	87
Bsp1286I	GDGCHC	1 cut(s)	1063
Bsp13I	TCCGGA	1 cut(s)	1256
Bsp1407I	TGTACA	1 cut(s)	2149
Bsp19I	CCATGG	2 cut(s)	686, 3259
Bsp68I	TCGCGA	1 cut(s)	3042
BspCNI	CTCAG	5 cut(s)	941, 1870, 2201, 2419, 2667
BspDI	ATCGAT	4 cut(s)	342, 1530, 1596, 2798
BspEI	TCCGGA	1 cut(s)	1256
BspFNI	CGCG	3 cut(s)	103, 379, 3042
BspLI	GGNNCC	8 cut(s)	190, 692, 850, 1395, 1687, 2078, 2256, 2406
BspMI	ACCTGC	1 cut(s)	1846
BspPI	GGATC	5 cut(s)	347, 605, 1680, 1693, 2189
BspQI	GCTCTTC	1 cut(s)	807
BspT104I	TTCGAA	1 cut(s)	87
BspT107I	GGYRCC	1 cut(s)	2404
BspTI	CTTAAG	1 cut(s)	2615
BsrDI	GCAATG	2 cut(s)	1867, 3200
BsrFI	RCCGGY	4 cut(s)	155, 227, 1185, 1584
BsrGI	TGTACA	1 cut(s)	2149
BsrI	ACTGG	7 cut(s)	556, 1048, 1394, 2176, 2338, 2781, 3306
BssAI	RCCGGY	4 cut(s)	155, 227, 1185, 1584
BssECI	CCNNGG	8 cut(s)	265, 686, 864, 1751, 2057, 2835, 3165, 3259
BssHII	GCGCGC	1 cut(s)	101
BssSI	CACGAG	2 cut(s)	1620, 1785
BssT1I	CCWWGG	4 cut(s)	686, 864, 2057, 3259
Bst2BI	CACGAG	2 cut(s)	1620, 1785
Bst2UI	CCWGG	3 cut(s)	1752, 2837, 3167
Bst6I	CTCTTC	1 cut(s)	807
BstAFI	CTTAAG	1 cut(s)	2615
BstAPI	GCANNNNNTGC	1 cut(s)	555
BstAUI	TGTACA	1 cut(s)	2149
BstBAI	YACGTR	2 cut(s)	2721, 2753
BstBI	TTCGAA	1 cut(s)	87
BstC8I	GCNNGC	5 cut(s)	103, 116, 272, 1995, 3040
BstDEI	CTNAG	8 cut(s)	706, 858, 928, 1878, 2188, 2383, 2427, 2675
BstDSI	CCRYGG	3 cut(s)	265, 686, 3259
BstENI	CCTNNNNNAGG	1 cut(s)	1877
BstFNI	CGCG	3 cut(s)	103, 379, 3042
BstH2I	RGCGCY	2 cut(s)	316, 1420
BstHHI	GCGC	5 cut(s)	103, 105, 315, 496, 1419
BstMAI	GTCTC	4 cut(s)	1116, 1994, 2092, 3148
BstMCI	CGRYCG	1 cut(s)	295
BstMWI	GCNNNNNNNGC	5 cut(s)	111, 555, 1232, 1552, 3035
BstNI	CCWGG	3 cut(s)	1752, 2837, 3167
BstNSI	RCATGY	1 cut(s)	3277
BstSCI	CCNGG	6 cut(s)	315, 410, 1338, 1750, 2835, 3165
BstSFI	CTRYAG	4 cut(s)	361, 595, 834, 1312
BstUI	CGCG	3 cut(s)	103, 379, 3042
BstV1I	GCAGC	4 cut(s)	1142, 2653, 2978, 3067
BstV2I	GAAGAC	2 cut(s)	121, 3080
BstX2I	RGATCY	3 cut(s)	646, 1292, 1685
BstYI	RGATCY	3 cut(s)	646, 1292, 1685
Bsu15I	ATCGAT	4 cut(s)	342, 1530, 1596, 2798
BsuTUI	ATCGAT	4 cut(s)	342, 1530, 1596, 2798
BtgI	CCRYGG	3 cut(s)	265, 686, 3259
BtrI	CACGTC	3 cut(s)	238, 325, 3100
BtsI	GCAGTG	1 cut(s)	1233
BtsIMutI	CAGTG	8 cut(s)	549, 962, 1173, 1233, 2145, 2183, 2247, 2424
BtuMI	TCGCGA	1 cut(s)	3042
BveI	ACCTGC	1 cut(s)	1846
Cac8I	GCNNGC	5 cut(s)	103, 116, 272, 1995, 3040
CaiI	CAGNNNCTG	2 cut(s)	2762, 3233
CfoI	GCGC	5 cut(s)	103, 105, 315, 496, 1419
Cfr10I	RCCGGY	4 cut(s)	155, 227, 1185, 1584
Cfr13I	GGNCC	7 cut(s)	69, 112, 140, 690, 849, 1452, 1625
ClaI	ATCGAT	4 cut(s)	342, 1530, 1596, 2798
CseI	GACGC	3 cut(s)	385, 2576, 3194
Csp6I	GTAC	8 cut(s)	349, 382, 831, 2062, 2150, 2331, 2705, 2774
CspCI	CAANNNNNGTGG	2 cut(s)	648, 683
CviAII	CATG	8 cut(s)	223, 538, 687, 1634, 1690, 2830, 3260, 3274
CviQI	GTAC	8 cut(s)	349, 382, 831, 2062, 2150, 2331, 2705, 2774
DdeI	CTNAG	8 cut(s)	706, 858, 928, 1878, 2188, 2383, 2427, 2675
DraI	TTTAAA	1 cut(s)	2895
EaeI	YGGCCR	4 cut(s)	172, 183, 229, 2569
Eam1104I	CTCTTC	1 cut(s)	807
EarI	CTCTTC	1 cut(s)	807
Eco130I	CCWWGG	4 cut(s)	686, 864, 2057, 3259
Eco147I	AGGCCT	2 cut(s)	359, 3110
Eco47I	GGWCC	4 cut(s)	69, 690, 849, 1625
Eco47III	AGCGCT	1 cut(s)	1418
Eco72I	CACGTG	1 cut(s)	2721
Eco88I	CYCGRG	1 cut(s)	760
EcoNI	CCTNNNNNAGG	1 cut(s)	1877
EcoRII	CCWGG	3 cut(s)	1750, 2835, 3165
EcoT14I	CCWWGG	4 cut(s)	686, 864, 2057, 3259
EcoT22I	ATGCAT	1 cut(s)	2071
ErhI	CCWWGG	4 cut(s)	686, 864, 2057, 3259
FaeI	CATG	8 cut(s)	226, 541, 690, 1637, 1693, 2833, 3263, 3277
FaqI	GGGAC	4 cut(s)	272, 332, 1054, 2818
FatI	CATG	8 cut(s)	222, 537, 686, 1633, 1689, 2829, 3259, 3273
FauI	CCCGC	2 cut(s)	171, 2962
FblI	GTMKAC	1 cut(s)	894
Fnu4HI	GCNGC	5 cut(s)	1156, 2667, 2786, 2967, 3081
Fsp4HI	GCNGC	5 cut(s)	1156, 2667, 2786, 2967, 3081
FspBI	CTAG	8 cut(s)	699, 807, 1247, 1367, 1523, 1775, 2141, 2204
FspI	TGCGCA	1 cut(s)	495
GlaI	GCGC	5 cut(s)	102, 104, 314, 495, 1418
GluI	GCNGC	5 cut(s)	1156, 2667, 2786, 2967, 3081
GsaI	CCCAGC	1 cut(s)	879
GsuI	CTGGAG	2 cut(s)	1309, 2886
HaeII	RGCGCY	2 cut(s)	316, 1420
HgaI	GACGC	3 cut(s)	385, 2576, 3194
HhaI	GCGC	5 cut(s)	103, 105, 315, 496, 1419
Hin1II	CATG	8 cut(s)	226, 541, 690, 1637, 1693, 2833, 3263, 3277
Hin6I	GCGC	5 cut(s)	101, 103, 313, 494, 1417
HinP1I	GCGC	5 cut(s)	101, 103, 313, 494, 1417
HincII	GTYRAC	1 cut(s)	895
HindII	GTYRAC	1 cut(s)	895
HindIII	AAGCTT	2 cut(s)	1544, 1553
HphI	GGTGA	7 cut(s)	199, 400, 637, 1523, 1914, 2348, 2990
Hpy166II	GTNNAC	2 cut(s)	895, 2774
Hpy188I	TCNGA	7 cut(s)	73, 991, 1782, 2088, 2470, 3229, 3290
Hpy8I	GTNNAC	2 cut(s)	895, 2774
Hpy99I	CGWCG	4 cut(s)	140, 326, 389, 1650
HpyF10VI	GCNNNNNNNGC	5 cut(s)	111, 555, 1232, 1552, 3035
HpyF3I	CTNAG	8 cut(s)	706, 858, 928, 1878, 2188, 2383, 2427, 2675
Hsp92II	CATG	8 cut(s)	226, 541, 690, 1637, 1693, 2833, 3263, 3277
HspAI	GCGC	5 cut(s)	101, 103, 313, 494, 1417
Kpn2I	TCCGGA	1 cut(s)	1256
LguI	GCTCTTC	1 cut(s)	807
LmnI	GCTCC	4 cut(s)	872, 1066, 1345, 2530
Lsp1109I	GCAGC	4 cut(s)	1142, 2653, 2978, 3067
LweI	GCATC	7 cut(s)	1192, 1945, 2632, 2953, 3175, 3210, 3220
MaeI	CTAG	8 cut(s)	699, 807, 1247, 1367, 1523, 1775, 2141, 2204
MfeI	CAATTG	4 cut(s)	95, 497, 636, 1426
MflI	RGATCY	3 cut(s)	646, 1292, 1685
MhlI	GDGCHC	1 cut(s)	1063
MlsI	TGGCCA	1 cut(s)	174
MluNI	TGGCCA	1 cut(s)	174
MlyI	GAGTC	5 cut(s)	292, 1777, 2209, 2474, 3023
MmeI	TCCRAC	3 cut(s)	404, 2242, 2670
Mox20I	TGGCCA	1 cut(s)	174
Mph1103I	ATGCAT	1 cut(s)	2071
MroI	TCCGGA	1 cut(s)	1256
MroXI	GAANNNNTTC	1 cut(s)	2478
MscI	TGGCCA	1 cut(s)	174
MseI	TTAA	9 cut(s)	978, 1566, 1938, 2411, 2616, 2700, 2894, 2910, 3087
MslI	CAYNNNNRTG	3 cut(s)	1805, 2429, 2640
Msp20I	TGGCCA	1 cut(s)	174
MspA1I	CMGCKG	2 cut(s)	2969, 3080
MspCI	CTTAAG	1 cut(s)	2615
MspR9I	CCNGG	6 cut(s)	317, 412, 1340, 1752, 2837, 3167
MunI	CAATTG	4 cut(s)	95, 497, 636, 1426
Mva1269I	GAATGC	2 cut(s)	2095, 2478
MvaI	CCWGG	3 cut(s)	1752, 2837, 3167
MvnI	CGCG	3 cut(s)	103, 379, 3042
MwoI	GCNNNNNNNGC	5 cut(s)	111, 555, 1232, 1552, 3035
NciI	CCSGG	3 cut(s)	317, 412, 1340
NcoI	CCATGG	2 cut(s)	686, 3259
NlaIII	CATG	8 cut(s)	226, 541, 690, 1637, 1693, 2833, 3263, 3277
NlaIV	GGNNCC	8 cut(s)	190, 692, 850, 1395, 1687, 2078, 2256, 2406
NmuCI	GTSAC	4 cut(s)	373, 625, 2588, 2716
NruI	TCGCGA	1 cut(s)	3042
NsbI	TGCGCA	1 cut(s)	495
NsiI	ATGCAT	1 cut(s)	2071
NspI	RCATGY	1 cut(s)	3277
NspV	TTCGAA	1 cut(s)	87
OliI	CACNNNNGTG	1 cut(s)	2429
PauI	GCGCGC	1 cut(s)	101
PceI	AGGCCT	2 cut(s)	359, 3110
PciSI	GCTCTTC	1 cut(s)	807
PcsI	WCGNNNNNNNCGW	1 cut(s)	132
PctI	GAATGC	2 cut(s)	2095, 2478
PdmI	GAANNNNTTC	1 cut(s)	2478
PfeI	GAWTC	8 cut(s)	213, 249, 473, 528, 785, 1170, 1532, 3119
PflFI	GACNNNGTC	1 cut(s)	1403
PflMI	CCANNNNNTGG	1 cut(s)	181
PkrI	GCNGC	5 cut(s)	1157, 2668, 2787, 2968, 3082
PleI	GAGTC	5 cut(s)	292, 1777, 2208, 2473, 3022
PmaCI	CACGTG	1 cut(s)	2721
PmlI	CACGTG	1 cut(s)	2721
PpsI	GAGTC	5 cut(s)	292, 1777, 2208, 2473, 3022
Ppu21I	YACGTR	2 cut(s)	2721, 2753
PshBI	ATTAAT	1 cut(s)	1938
PsiI	TTATAA	1 cut(s)	522
Psp6I	CCWGG	3 cut(s)	1750, 2835, 3165
PspCI	CACGTG	1 cut(s)	2721
PspFI	CCCAGC	1 cut(s)	875
PspGI	CCWGG	3 cut(s)	1750, 2835, 3165
PspN4I	GGNNCC	8 cut(s)	190, 692, 850, 1395, 1687, 2078, 2256, 2406
PspPI	GGNCC	7 cut(s)	69, 112, 140, 690, 849, 1452, 1625
PsrI	GAACNNNNNNTAC	2 cut(s)	2688, 2720
PstNI	CAGNNNCTG	2 cut(s)	2762, 3233
PsuI	RGATCY	3 cut(s)	646, 1292, 1685
PsyI	GACNNNGTC	1 cut(s)	1403
PteI	GCGCGC	1 cut(s)	101
PvuII	CAGCTG	1 cut(s)	3080
RruI	TCGCGA	1 cut(s)	3042
RsaI	GTAC	8 cut(s)	350, 383, 832, 2063, 2151, 2332, 2706, 2775
RsaNI	GTAC	8 cut(s)	349, 382, 831, 2062, 2150, 2331, 2705, 2774
RseI	CAYNNNNRTG	3 cut(s)	1805, 2429, 2640
SalI	GTCGAC	1 cut(s)	893
SapI	GCTCTTC	1 cut(s)	807
SaqAI	TTAA	9 cut(s)	978, 1566, 1938, 2411, 2616, 2700, 2894, 2910, 3087
SatI	GCNGC	5 cut(s)	1156, 2667, 2786, 2967, 3081
Sau96I	GGNCC	7 cut(s)	69, 112, 140, 690, 849, 1452, 1625
ScaI	AGTACT	1 cut(s)	832
SchI	GAGTC	5 cut(s)	292, 1777, 2209, 2474, 3023
ScrFI	CCNGG	6 cut(s)	317, 412, 1340, 1752, 2837, 3167
SduI	GDGCHC	1 cut(s)	1063
SfaNI	GCATC	7 cut(s)	1192, 1945, 2632, 2953, 3175, 3210, 3220
SfcI	CTRYAG	4 cut(s)	361, 595, 834, 1312
SfuI	TTCGAA	1 cut(s)	87
SinI	GGWCC	4 cut(s)	69, 690, 849, 1625
SmiMI	CAYNNNNRTG	3 cut(s)	1805, 2429, 2640
SmlI	CTYRAG	3 cut(s)	2603, 2615, 3213
SmoI	CTYRAG	3 cut(s)	2603, 2615, 3213
SpeI	ACTAGT	1 cut(s)	1522
SseBI	AGGCCT	2 cut(s)	359, 3110
SspI	AATATT	1 cut(s)	2293
SspMI	CTAG	8 cut(s)	699, 807, 1247, 1367, 1523, 1775, 2141, 2204
StuI	AGGCCT	2 cut(s)	359, 3110
StyD4I	CCNGG	6 cut(s)	315, 410, 1338, 1750, 2835, 3165
StyI	CCWWGG	4 cut(s)	686, 864, 2057, 3259
TatI	WGTACW	4 cut(s)	348, 830, 2149, 2773
TauI	GCSGC	1 cut(s)	2788
TfiI	GAWTC	8 cut(s)	213, 249, 473, 528, 785, 1170, 1532, 3119
Tru1I	TTAA	9 cut(s)	978, 1566, 1938, 2411, 2616, 2700, 2894, 2910, 3087
Tru9I	TTAA	9 cut(s)	978, 1566, 1938, 2411, 2616, 2700, 2894, 2910, 3087
TscAI	CASTG	8 cut(s)	556, 969, 1180, 1233, 2152, 2183, 2254, 2431
TseFI	GTSAC	4 cut(s)	373, 625, 2588, 2716
TseI	GCWGC	4 cut(s)	1155, 2666, 2966, 3080
Tsp45I	GTSAC	4 cut(s)	373, 625, 2588, 2716
TspDTI	ATGAA	7 cut(s)	891, 927, 1043, 2124, 2243, 2535, 2624
TspGWI	ACGGA	3 cut(s)	122, 135, 3085
TspRI	CASTG	8 cut(s)	556, 969, 1180, 1233, 2152, 2183, 2254, 2431
Tth111I	GACNNNGTC	1 cut(s)	1403
Van91I	CCANNNNNTGG	1 cut(s)	181
Vha464I	CTTAAG	1 cut(s)	2615
VpaK11BI	GGWCC	4 cut(s)	69, 690, 849, 1625
VspI	ATTAAT	1 cut(s)	1938
XagI	CCTNNNNNAGG	1 cut(s)	1877
XapI	RAATTY	5 cut(s)	574, 923, 1744, 2443, 2454
XceI	RCATGY	1 cut(s)	3277
XmiI	GTMKAC	1 cut(s)	894
XmnI	GAANNNNTTC	1 cut(s)	2478
XspI	CTAG	8 cut(s)	699, 807, 1247, 1367, 1523, 1775, 2141, 2204
ZrmI	AGTACT	1 cut(s)	832
Zsp2I	ATGCAT	1 cut(s)	2071

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.