pycom111g05550

Alpha-amylase C-terminal beta-sheet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0003013
Physical Location & Seq
Reverse (-)
3546463 .. 3549897
3435 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2061 bp
ATGGCAGTTGGGGTTATTCACCACTGCCCCATTGCATCTTCAGGTCCACATTGCAAGTATGATAGGAGTGTATGCCGCCTGGGGCGGCGTCCTCTTGTCCTCAGAACTAATTCGAATAGAAAAAAGAACCTTTTCTACACGCCACATTGGCTATGTAAATCAAGAACTAGTATTTTTTCAAGCATGGATGATTCCACTGACACTTTTACCGACGTGGCTAACACTTCAGGTAGTAGTGAAGTGTTGAATATAGAAGAAGATGAATTGATGACAGCTAGGAAAGCTCTTTTGGAGGCCCAAGCTAGACAAGAAGCCATTGAGAAAGAGAGAGATCAATTGCTTGAAGAGTTAGCATGTTCCGAGGCTAAACAACAGGAATATGTTGCTACCATATTGCATGACAAGGAATTGATTATTGCAGAACTTGAGGGAGCCAAGTCACTGTTCCATCAGAAGCTGCTGGAATCAGTTGAAGAGAAGTTCAGCTTAGAGTCTAAGTTAGTTCTTGCAAAACAGGATGCGGTTGAACTTGCGGTACAAGTAGAAAAGTTAGCGGAAATTGCTTTCCAGCAGGCCACATCTCACATTCTACAAGATGCCCAGATGAGGGTTTCAGCGGCAGAAACTACAGCTGCTGAAGCAGCTTATCAGATAGAAAAACAAATTAAGGAGGTGACTGAAGGTAGTATATTGTTAATTGTGGAACAGTCAAAACTTGCAATAGAGAAGGCACTGGATGCGGCGGAAAAATCTGGTGAGTATGCATCAAAAGCAGTACTAGAATATACTGAAGGTGTGAGTCCACTGGATGAGCTTGCTTCCCTTCAGTCAAAAAATATTATGTTACAAGGTGCCGTAAATGATTTAGAATCTCAGTTGTTGCTGACAAGAAGTGACGTTGATAGGTTGAAGTTGGAGTTGGAAAAGGCCCTTGCACATGCAAATGCGTTTGAGGTCCGAGCTAATGATGCTGAGAAAGCATTGCTTGAATTTCAGGAATCCAGTAGGAAAAATACACTCCAGAAAGAGGAGGAAATTATGTCTTTGATAGAGAAAATAAAGAAAGATACGTCAGAAAGAAAGAAGTCTTCTTCTAAGGCGTTCAAGGCTGAGTTGCAAAGCATTAGGGATGCTATTGGAGCTGCCAAAGAAATGGCACGTACGAAAGATGATGCATACTTGAGAAGATGTGAAGCACTGCGAAGATCACTGAAGGCATCTGAAGCTGCTACAAAGATGTGGAGACAGAGAGCAGAAATGGCAGAATCATTATTATTGAAGGACAGATCACTGGGTGAGGGGGATGAAGATTCTATTTATGTTGTTAATGGTGGGCGGATAGACCTTTTGACAGATGATGATTCACAGAAATGGAAACTTATAAGTGATGGCCCTCGCCGAGAGATACCTCAATGGATGGCTAGGAAAATTCGTACCATCAGTCCCAGGTTCCCTCCAAGAAAGATCGATGTAGCTGAAGCCTCGAGTTCAAAGTTCAGATCTTTGAATTTGCCCAAACTTGATGAAGTATGGTCTATAGCTAAGGAAAAGCCAAAGGAGGGCGATACACTTATTGAGCATGTGCGTGAGAAAGAAACAATAGAAAAGAAACGAAAAGCTCTGGAGCACGTTCTTCAGAGGAAGACCATACAATGGCAGAGCACCCAGGAACAAACAAAGTTAGAGCCAGGAACTGGAACCGGACGTGAGATCGTGTTTCAAGGCTTCAATTGGGAAAGCTGGAGAAGGCAGTGGTACTTAGACTTAGCTCCTAAAGCTGCTGATTTATCTAAAATTGGGGTAACGGCAGTGTGGTTCCCACCACCAACAGAATCTGTCGCTCCTCAAGGTTATATGCCTTCGGACCTTTACAATTTGAACTCAGCATATGGTACTGTGGATGAACTTAAACACTGCATTGAGGAAATGCATTCTCAAGATCTTCTGGCCCTGGGAGATGTTGTCCTAAATCATAGATGTGCTCATAAACAGAGTCCAAACGGCATTTGGAACATTTTTGGTGGGAAGCTTGCTTGGGGGCCTGAAGCAATTGTATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko04973 map00500 map01100 map04973
Pfam Domains
Protein Families
Alpha-amyl_C2 Alpha-amylase

Protein Analysis

687

Amino Acids

77.3

Weight (kDa)

5.81

Isoelectric Point (pI)

55.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha-amylase PF00128 596 - 660 2.8e-09 Alpha amylase, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1382
AccB1I GGYRCC 1 cut(s) 851
AccB7I CCANNNNNTGG 2 cut(s) 1653, 1694
AciI CCGC 9 cut(s) 76, 85, 521, 533, 554, 617, 740, 743, 1336
AcsI RAATTY 3 cut(s) 989, 1428, 1507
AcyI GRCGYC 1 cut(s) 88
AdeI CACNNNGTG 1 cut(s) 1295
AfaI GTAC 6 cut(s) 537, 777, 1162, 1435, 1757, 1894
AfiI CCNNNNNNNGG 6 cut(s) 606, 607, 1027, 1559, 1653, 1694
AhlI ACTAGT 1 cut(s) 167
AjiI CACGTC 2 cut(s) 214, 1706
AjnI CCWGG 5 cut(s) 78, 1445, 1665, 1687, 1950
AjuI GAANNNNNNNTTGG 6 cut(s) 272, 304, 428, 460, 902, 934
Alw21I GWGCWC 3 cut(s) 1629, 1664, 1984
Alw26I GTCTC 1 cut(s) 1237
AlwNI CAGNNNCTG 4 cut(s) 457, 635, 1694, 1835
Ama87I CYCGRG 1 cut(s) 1483
AoxI GGCC 6 cut(s) 294, 573, 927, 1390, 1947, 2039
ApeKI GCWGC 6 cut(s) 457, 632, 641, 1142, 1226, 1778
ApoI RAATTY 3 cut(s) 989, 1428, 1507
Asp700I GAANNNNTTC 2 cut(s) 109, 131
AspS9I GGNCC 8 cut(s) 44, 295, 928, 955, 1391, 1864, 1948, 2039
AsuHPI GGTGA 4 cut(s) 11, 685, 767, 1307
AsuII TTCGAA 1 cut(s) 113
AvaI CYCGRG 1 cut(s) 1483
AvaII GGWCC 3 cut(s) 44, 955, 1864
BanI GGYRCC 1 cut(s) 851
BbsI GAAGAC 2 cut(s) 1080, 1649
Bbv12I GWGCWC 3 cut(s) 1629, 1664, 1984
BbvI GCAGC 6 cut(s) 444, 619, 653, 1129, 1213, 1765
BccI CCATC 4 cut(s) 456, 1382, 1411, 1445
BceAI ACGGC 3 cut(s) 839, 1821, 2017
BciT130I CCWGG 5 cut(s) 80, 1447, 1667, 1689, 1952
BcoDI GTCTC 1 cut(s) 1237
BcuI ACTAGT 1 cut(s) 167
BfaI CTAG 5 cut(s) 168, 276, 303, 779, 1422
BfmI CTRYAG 2 cut(s) 627, 1536
BglI GCCNNNNNGGC 1 cut(s) 148
BglII AGATCT 2 cut(s) 1499, 1939
BmcAI AGTACT 1 cut(s) 777
Bme1390I CCNGG 5 cut(s) 80, 1447, 1667, 1689, 1952
Bme18I GGWCC 3 cut(s) 44, 955, 1864
BmeT110I CYCGRG 1 cut(s) 1483
BmgBI CACGTC 2 cut(s) 214, 1706
BmgT120I GGNCC 8 cut(s) 44, 295, 928, 955, 1391, 1864, 1948, 2039
BmiI GGNNCC 6 cut(s) 433, 853, 1451, 1699, 1817, 2040
BmrFI CCNGG 5 cut(s) 80, 1447, 1667, 1689, 1952
BmrI ACTGGG 1 cut(s) 1301
BmsI GCATC 9 cut(s) 44, 508, 586, 727, 773, 958, 1120, 1162, 1226
BmuI ACTGGG 1 cut(s) 1301
BpiI GAAGAC 2 cut(s) 1080, 1649
BpmI CTGGAG 3 cut(s) 1004, 1643, 1762
Bpu10I CCTNAGC 1 cut(s) 1542
Bpu14I TTCGAA 1 cut(s) 113
BpuEI CTTGAG 4 cut(s) 446, 1201, 1830, 1920
Bsa29I ATCGAT 1 cut(s) 1467
BsaAI YACGTR 1 cut(s) 1160
BsaHI GRCGYC 1 cut(s) 88
BsaJI CCNNGG 6 cut(s) 79, 360, 1445, 1665, 1950, 1951
BsaWI WCCGGW 1 cut(s) 1700
BsaXI ACNNNNNCTCC 2 cut(s) 1947, 1977
Bsc4I CCNNNNNNNGG 6 cut(s) 606, 607, 1027, 1559, 1653, 1694
Bse1I ACTGG 5 cut(s) 738, 810, 1002, 1296, 1699
Bse3DI GCAATG 3 cut(s) 30, 49, 980
BseBI CCWGG 5 cut(s) 80, 1447, 1667, 1689, 1952
BseCI ATCGAT 1 cut(s) 1467
BseDI CCNNGG 6 cut(s) 79, 360, 1445, 1665, 1950, 1951
BseGI GGATG 8 cut(s) 193, 523, 742, 814, 1135, 1309, 1422, 1906
BseLI CCNNNNNNNGG 6 cut(s) 606, 607, 1027, 1559, 1653, 1694
BseMI GCAATG 3 cut(s) 30, 49, 980
BseMII CTCAG 5 cut(s) 115, 887, 963, 1101, 1896
BseNI ACTGG 5 cut(s) 738, 810, 1002, 1296, 1699
BseRI GAGGAG 2 cut(s) 1043, 1833
BseXI GCAGC 6 cut(s) 444, 619, 653, 1129, 1213, 1765
BshFI GGCC 6 cut(s) 296, 575, 929, 1392, 1949, 2041
BshNI GGYRCC 1 cut(s) 851
BshVI ATCGAT 1 cut(s) 1467
BsiHKAI GWGCWC 3 cut(s) 1629, 1664, 1984
BsiHKCI CYCGRG 1 cut(s) 1483
BsiSI CCGG 1 cut(s) 1701
BsiWI CGTACG 1 cut(s) 1160
BslFI GGGAC 1 cut(s) 1428
BslI CCNNNNNNNGG 6 cut(s) 606, 607, 1027, 1559, 1653, 1694
BsmAI GTCTC 1 cut(s) 1237
BsmFI GGGAC 1 cut(s) 1428
BsmI GAATGC 1 cut(s) 1930
BsnI GGCC 6 cut(s) 296, 575, 929, 1392, 1949, 2041
BsoBI CYCGRG 1 cut(s) 1483
Bsp119I TTCGAA 1 cut(s) 113
Bsp1286I GDGCHC 3 cut(s) 1629, 1664, 1984
Bsp143I GATC 7 cut(s) 331, 1205, 1286, 1464, 1499, 1710, 1939
BspACI CCGC 9 cut(s) 76, 85, 521, 533, 554, 617, 740, 743, 1336
BspANI GGCC 6 cut(s) 296, 575, 929, 1392, 1949, 2041
BspCNI CTCAG 5 cut(s) 114, 886, 964, 1102, 1895
BspDI ATCGAT 1 cut(s) 1467
BspLI GGNNCC 6 cut(s) 433, 853, 1451, 1699, 1817, 2040
BspT104I TTCGAA 1 cut(s) 113
BspT107I GGYRCC 1 cut(s) 851
BsrDI GCAATG 3 cut(s) 30, 49, 980
BsrI ACTGG 5 cut(s) 738, 810, 1002, 1296, 1699
BssECI CCNNGG 6 cut(s) 79, 360, 1445, 1665, 1950, 1951
BssMI GATC 7 cut(s) 331, 1205, 1286, 1464, 1499, 1710, 1939
BssNI GRCGYC 1 cut(s) 88
Bst2UI CCWGG 5 cut(s) 80, 1447, 1667, 1689, 1952
Bst4CI ACNGT 3 cut(s) 444, 708, 1897
Bst6I CTCTTC 2 cut(s) 339, 468
BstACI GRCGYC 1 cut(s) 88
BstAPI GCANNNNNTGC 1 cut(s) 737
BstBAI YACGTR 1 cut(s) 1160
BstBI TTCGAA 1 cut(s) 113
BstC8I GCNNGC 3 cut(s) 573, 816, 2031
BstF5I GGATG 8 cut(s) 193, 523, 742, 814, 1135, 1309, 1422, 1906
BstKTI GATC 7 cut(s) 334, 1208, 1289, 1467, 1502, 1713, 1942
BstMAI GTCTC 1 cut(s) 1237
BstMBI GATC 7 cut(s) 331, 1205, 1286, 1464, 1499, 1710, 1939
BstNI CCWGG 5 cut(s) 80, 1447, 1667, 1689, 1952
BstNSI RCATGY 3 cut(s) 357, 941, 1583
BstSCI CCNGG 5 cut(s) 78, 1445, 1665, 1687, 1950
BstSFI CTRYAG 2 cut(s) 627, 1536
BstV1I GCAGC 6 cut(s) 444, 619, 653, 1129, 1213, 1765
BstV2I GAAGAC 2 cut(s) 1080, 1649
BstX2I RGATCY 2 cut(s) 1499, 1939
BstXI CCANNNNNNTGG 1 cut(s) 1153
BstYI RGATCY 2 cut(s) 1499, 1939
Bsu15I ATCGAT 1 cut(s) 1467
BsuRI GGCC 6 cut(s) 296, 575, 929, 1392, 1949, 2041
BsuTUI ATCGAT 1 cut(s) 1467
BtrI CACGTC 2 cut(s) 214, 1706
BtsCI GGATG 8 cut(s) 193, 523, 742, 814, 1135, 1309, 1422, 1906
BtsI GCAGTG 5 cut(s) 22, 1196, 1757, 1815, 1912
Cac8I GCNNGC 3 cut(s) 573, 816, 2031
CaiI CAGNNNCTG 4 cut(s) 457, 635, 1694, 1835
Cfr13I GGNCC 8 cut(s) 44, 295, 928, 955, 1391, 1864, 1948, 2039
ClaI ATCGAT 1 cut(s) 1467
CseI GACGC 1 cut(s) 77
Csp6I GTAC 6 cut(s) 536, 776, 1161, 1434, 1756, 1893
CviAII CATG 5 cut(s) 184, 354, 398, 938, 1580
CviQI GTAC 6 cut(s) 536, 776, 1161, 1434, 1756, 1893
DpnI GATC 7 cut(s) 333, 1207, 1288, 1466, 1501, 1712, 1941
DpnII GATC 7 cut(s) 331, 1205, 1286, 1464, 1499, 1710, 1939
DraIII CACNNNGTG 1 cut(s) 1295
Eam1104I CTCTTC 2 cut(s) 339, 468
EarI CTCTTC 2 cut(s) 339, 468
EciI GGCGGA 2 cut(s) 758, 1351
Eco47I GGWCC 3 cut(s) 44, 955, 1864
Eco88I CYCGRG 1 cut(s) 1483
EcoO109I RGGNCCY 2 cut(s) 928, 2039
EcoRII CCWGG 5 cut(s) 78, 1445, 1665, 1687, 1950
EcoT22I ATGCAT 3 cut(s) 766, 1177, 1932
FaeI CATG 5 cut(s) 187, 357, 401, 941, 1583
FalI AAGNNNNNCTT 4 cut(s) 470, 502, 969, 1001
FaqI GGGAC 1 cut(s) 1428
FatI CATG 5 cut(s) 183, 353, 397, 937, 1579
FauNDI CATATG 1 cut(s) 1888
FokI GGATG 8 cut(s) 200, 530, 749, 821, 1142, 1316, 1429, 1913
FspBI CTAG 5 cut(s) 168, 276, 303, 779, 1422
GsuI CTGGAG 3 cut(s) 1004, 1643, 1762
HaeIII GGCC 6 cut(s) 296, 575, 929, 1392, 1949, 2041
HapII CCGG 1 cut(s) 1701
HgaI GACGC 1 cut(s) 77
Hin1I GRCGYC 1 cut(s) 88
Hin1II CATG 5 cut(s) 187, 357, 401, 941, 1583
HindIII AAGCTT 1 cut(s) 2027
HpaII CCGG 1 cut(s) 1701
HphI GGTGA 4 cut(s) 11, 685, 767, 1307
Hpy166II GTNNAC 2 cut(s) 47, 803
Hpy188III TCNNGA 5 cut(s) 162, 995, 1021, 1622, 1937
Hpy8I GTNNAC 2 cut(s) 47, 803
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 8 cut(s) 674, 721, 785, 833, 1207, 1273, 1740, 1869
HpyCH4III ACNGT 3 cut(s) 444, 708, 1897
HpyCH4IV ACGT 6 cut(s) 213, 897, 1070, 1159, 1629, 1705
HpySE526I ACGT 6 cut(s) 213, 897, 1070, 1159, 1629, 1705
Hsp92I GRCGYC 1 cut(s) 88
Hsp92II CATG 5 cut(s) 187, 357, 401, 941, 1583
Kzo9I GATC 7 cut(s) 331, 1205, 1286, 1464, 1499, 1710, 1939
LmnI GCTCC 5 cut(s) 431, 1139, 1624, 1774, 1846
Lsp1109I GCAGC 6 cut(s) 444, 619, 653, 1129, 1213, 1765
LweI GCATC 9 cut(s) 44, 508, 586, 727, 773, 958, 1120, 1162, 1226
MaeI CTAG 5 cut(s) 168, 276, 303, 779, 1422
MaeII ACGT 6 cut(s) 213, 897, 1070, 1159, 1629, 1705
MaeIII GTNAC 5 cut(s) 438, 673, 843, 893, 1801
MalI GATC 7 cut(s) 333, 1207, 1288, 1466, 1501, 1712, 1941
MboI GATC 7 cut(s) 331, 1205, 1286, 1464, 1499, 1710, 1939
MfeI CAATTG 3 cut(s) 335, 1729, 2049
MflI RGATCY 2 cut(s) 1499, 1939
MhlI GDGCHC 3 cut(s) 1629, 1664, 1984
MlyI GAGTC 3 cut(s) 500, 808, 2002
MmeI TCCRAC 2 cut(s) 894, 900
Mph1103I ATGCAT 3 cut(s) 766, 1177, 1932
MroXI GAANNNNTTC 2 cut(s) 109, 131
MseI TTAA 4 cut(s) 666, 695, 1326, 1908
MslI CAYNNNNRTG 4 cut(s) 942, 1369, 1584, 1977
MspA1I CMGCKG 2 cut(s) 617, 632
MspI CCGG 1 cut(s) 1701
MspR9I CCNGG 5 cut(s) 80, 1447, 1667, 1689, 1952
MunI CAATTG 3 cut(s) 335, 1729, 2049
Mva1269I GAATGC 1 cut(s) 1930
MvaI CCWGG 5 cut(s) 80, 1447, 1667, 1689, 1952
NdeI CATATG 1 cut(s) 1888
NdeII GATC 7 cut(s) 331, 1205, 1286, 1464, 1499, 1710, 1939
NlaIII CATG 5 cut(s) 187, 357, 401, 941, 1583
NlaIV GGNNCC 6 cut(s) 433, 853, 1451, 1699, 1817, 2040
NmeAIII GCCGAG 1 cut(s) 1424
NmuCI GTSAC 3 cut(s) 438, 673, 893
NsiI ATGCAT 3 cut(s) 766, 1177, 1932
NspI RCATGY 3 cut(s) 357, 941, 1583
NspV TTCGAA 1 cut(s) 113
PaeR7I CTCGAG 1 cut(s) 1483
PasI CCCWGGG 1 cut(s) 1951
PctI GAATGC 1 cut(s) 1930
PdmI GAANNNNTTC 2 cut(s) 109, 131
PfeI GAWTC 8 cut(s) 191, 464, 869, 998, 1265, 1310, 1361, 1832
Pfl23II CGTACG 1 cut(s) 1160
PflMI CCANNNNNTGG 2 cut(s) 1653, 1694
PleI GAGTC 3 cut(s) 499, 807, 2001
PpsI GAGTC 3 cut(s) 499, 807, 2001
Ppu21I YACGTR 1 cut(s) 1160
PsiI TTATAA 1 cut(s) 1382
Psp6I CCWGG 5 cut(s) 78, 1445, 1665, 1687, 1950
PspGI CCWGG 5 cut(s) 78, 1445, 1665, 1687, 1950
PspLI CGTACG 1 cut(s) 1160
PspN4I GGNNCC 6 cut(s) 433, 853, 1451, 1699, 1817, 2040
PspPI GGNCC 8 cut(s) 44, 295, 928, 955, 1391, 1864, 1948, 2039
PspXI VCTCGAGB 1 cut(s) 1483
PsrI GAACNNNNNNTAC 4 cut(s) 119, 151, 519, 551
PstNI CAGNNNCTG 4 cut(s) 457, 635, 1694, 1835
PsuI RGATCY 2 cut(s) 1499, 1939
PvuII CAGCTG 1 cut(s) 632
RsaI GTAC 6 cut(s) 537, 777, 1162, 1435, 1757, 1894
RsaNI GTAC 6 cut(s) 536, 776, 1161, 1434, 1756, 1893
RseI CAYNNNNRTG 4 cut(s) 942, 1369, 1584, 1977
SaqAI TTAA 4 cut(s) 666, 695, 1326, 1908
Sau3AI GATC 7 cut(s) 331, 1205, 1286, 1464, 1499, 1710, 1939
Sau96I GGNCC 8 cut(s) 44, 295, 928, 955, 1391, 1864, 1948, 2039
ScaI AGTACT 1 cut(s) 777
SchI GAGTC 3 cut(s) 500, 808, 2002
ScrFI CCNGG 5 cut(s) 80, 1447, 1667, 1689, 1952
SduI GDGCHC 3 cut(s) 1629, 1664, 1984
SfaNI GCATC 9 cut(s) 44, 508, 586, 727, 773, 958, 1120, 1162, 1226
SfcI CTRYAG 2 cut(s) 627, 1536
Sfr274I CTCGAG 1 cut(s) 1483
SfuI TTCGAA 1 cut(s) 113
SinI GGWCC 3 cut(s) 44, 955, 1864
SlaI CTCGAG 1 cut(s) 1483
SmiMI CAYNNNNRTG 4 cut(s) 942, 1369, 1584, 1977
SmlI CTYRAG 5 cut(s) 425, 1180, 1483, 1845, 1935
SmoI CTYRAG 5 cut(s) 425, 1180, 1483, 1845, 1935
SpeI ACTAGT 1 cut(s) 167
SsiI CCGC 9 cut(s) 76, 85, 521, 533, 554, 617, 740, 743, 1336
SspI AATATT 1 cut(s) 838
SspMI CTAG 5 cut(s) 168, 276, 303, 779, 1422
StyD4I CCNGG 5 cut(s) 78, 1445, 1665, 1687, 1950
TaaI ACNGT 3 cut(s) 444, 708, 1897
TaiI ACGT 6 cut(s) 216, 900, 1073, 1162, 1632, 1708
TaqI TCGA 3 cut(s) 113, 1467, 1484
TatI WGTACW 1 cut(s) 775
TauI GCSGC 4 cut(s) 78, 88, 620, 743
TfiI GAWTC 8 cut(s) 191, 464, 869, 998, 1265, 1310, 1361, 1832
Tru1I TTAA 4 cut(s) 666, 695, 1326, 1908
Tru9I TTAA 4 cut(s) 666, 695, 1326, 1908
TseFI GTSAC 3 cut(s) 438, 673, 893
TseI GCWGC 6 cut(s) 457, 632, 641, 1142, 1226, 1778
Tsp45I GTSAC 3 cut(s) 438, 673, 893
TspDTI ATGAA 4 cut(s) 276, 1320, 1539, 1917
Van91I CCANNNNNTGG 2 cut(s) 1653, 1694
VpaK11BI GGWCC 3 cut(s) 44, 955, 1864
XapI RAATTY 3 cut(s) 989, 1428, 1507
XceI RCATGY 3 cut(s) 357, 941, 1583
XcmI CCANNNNNNNNNTGG 1 cut(s) 2004
XhoI CTCGAG 1 cut(s) 1483
XmnI GAANNNNTTC 2 cut(s) 109, 131
XspI CTAG 5 cut(s) 168, 276, 303, 779, 1422
ZrmI AGTACT 1 cut(s) 777
Zsp2I ATGCAT 3 cut(s) 766, 1177, 1932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.