pycom11g12760

FES

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0007319
Physical Location & Seq
Forward (+)
11720150 .. 11728731
8582 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4173 bp
ATGGCCGAACTCCAAGTATACCAGAGGAGAGAACCTGTTTCCAGAGACAATGCCATGGAAGAAGAAGCGAAAGAGGAGGTGGTTCAGAATCGGAAGCAGGGTTTGTGGGTGCCACAAACCCCTGCAAAGCCTGCTACAGGGAGGACACAAAAATCATATTTCAGAAGACGATTCAAGAGCAATTCGGGACAAAATAATAGCGTAGTGGTGAGTGATTCCCAAGTGGAATTGGAGCGTTCTGATGGGGTTTCTGTGTTTTCTGCTGCTGCAACTTCTTCAGAGGAAAACACTTGCCTGATCGACCCAATTGAAGAATGTCCTAGAGAGGAGGAAAAGGTACAGAATTTGGGTGGAATTGGAAGCACAAATTTGAGTTCTGTTGGGTTGTTCGATTTGAATAAGATTGTGTTGGAATGTGATGAGGGTTGTGTGAGTTTTTCTCATGGGGGTTCCGAGGATGCAAGCCAAGATTGTTTAGAGGGATTAGTGGCAGCAGAAGAGGTTGCGGCGAATGATGGTGAAATTTTGACAGTTGGGGAGGAGCTGGTGTTGCAGGGGATAATTGTAAAACCTCCAATTTCTCAGTCATCAACAAATGCAACTAAAAGGGGTTTATTTGAAAAGATAAACGGTTGTTTCATTCCGGTTGCACAAACTCGTAGCAATGAAGAAAAGCTGCAGCAGGGGTCTGCCATTCCAGATGGTATGTCGCAATGCAAGTCCGGTGATCAACAAAATCAAGAAAGTGTTAAGCAGGAGGATGTCATCCATCGGGCAGAAAACAAGAATCGGACGTCCAATGCGGCAAATTTTATGGGAGGCTCCAAATTTCCTAGCACTTCGAGCCCATCTCAGAAAAAGAAAAACTTGAAAAGAAGCCCAGATGGTGGTATAGATTTGAATGAAGGGCCACAAAAGAAACCGAAGAAGAAAGTATATAGGCCGAAAGTGGTATGTATAGGAAGACCAAGGCCTATAGAGCCTAAGACCCCAAAGAAGGCAACACCAAAGCGTCCTGCTTCAAAACCAAAGACACCAAAGCCTACAACTAAAATGCATGTTTCTTCCGAGCAAAATATGTCAGCCGAGAAGTCTTCAAGCAGCGCCAAACACTTGGCCCCCATAGAAGTCACCTTGGGTAAGGTTGCATTTTCTGTATCAGACGGAGCAACTCTAGATGGTGACCTGAAAATTAAATCAAACAAGGAGAATGCCATCATTCTAGCGACTCCTCCACAAAAAGTTAGAGCTAGTCACGGAACACTTATGAATCTCAGTTGTAGAAAAAATTTAGGACTGAACATTCCAGCTACATGCAGGAAACAAAGGTTGGCGAGGAGGAGGCGGGAAGAAGATAGTTGGTCGACAATAGCTAGATATTTTCTTGAGAGGGAAAGAGACCTTCTCAAAGGGCTTGGTGACAAAAATCAGGGACATGCTTCATTCCAAAAAACAGAAACCACAGATCTAATTGGTGCACTGCAGCCACTGATTATGACAAGGAAGAAAAGATCAAAGGGACATACTCGGGGACGGAATGGAGGGTTGGTGACAGAGAAATGGACTAACATGGAACATTCCCTCAATATAGAGCTTCACAATGGTGAATGTGAAGGACATCCATCTAAGCATGAGGGCCCTTGCTCCACCAACATAAGAGATTCACAGGAAGATAATATCAGTCCGTTTGTACAGAAATGTCCAGATATCAGCCATGGAGTTAACCATGACAAAGATGCACATGTTCCCAGCAATGACCATGGCATTCTAGTTCCATACAAGGACAAGCACAGTAAAAGGCGTCCAGAGCTTTCTCCCACAGACCTTGAACGAGTTAAGTCATGGAAACTTCAATTACAAGCGAAGGTTAATGAGGGTCAAGAGAAAGAAAGTGAAGAGGAATGGTGGAAGAATGAAAGAGAAGTCTTACATGGACGGATTAATCTACTTATGTCCCGGATGAACACAATCCAAGGAGATAGGAAATTTTCACCCTGGAAAGGTTCTGTGGTGGACTCAATAGTTGGAGTTTTCCTAACTCAAAATGTGTCGGATCATCTTTCAAGCAACGCCTTCTTGTCACTTGCTGCCAGATTCCCTTGTCAGTCCCATTCCAACGAAACAGATTGTGAATACACAAATATGGTTGTCACCCAAGAATCAGTTGGAAGTAATATTCTTGCTATCATGCCAAAATCTGAGGAGGAGTTACACACAGGTGAAGTGGCACTCTCAATGGACTATAGACACAAAGAGTCAAGCTCTGACAAACCCAATGACCTTGAAGGGTCGTCAACGCCTTTGTGTGATTTCCACAACCCTCCTGAGGTTTCCCAAATTGGTTTCAGTGAAAAGAATATGCTGGTGGATAAGCCTGAGAGCCCAGTTGATACTATAATCCAAAATCTTGTGGAACCACCTAATGAGGCCAACAACTTAAACCAGGTAAATCAGAAAGGAGAGAAACCGGATTTAAATGATCAACAATCAGACATGAAATTTGATAAGACGACGAAAGCTCCTCGAAGAAGAAAAAACAAGAAGGCTATAGTGAAGACTAAACTTAGTTGGGGGTGGTGTAAAAGTATATTTTCAACCAGTAGAGAAAGAAATAGGAATCACATGGATTCAGCAGACTGGGAGGCAGTTAGAATTGCAGAAGTTGGTCAGATTGCTGCGGCTATTAAAGTGCGTGGCCAACACAATATGATTGCAGGACGAATCAAGAAATTTCTTAACCAAGTACATGAGGATCATAAACAAATAGACCTCGAGTGGTTAAGAAATGCCCCGCCTCAGTTAGTAACGAAGTACCTAAGAGATATTGAGGGCATTGGATTGAAAAGCATGGAGTGTGTGAGACTTTTGGCACTCCAACATGTAGCTTTCCCGGTGGATGTAAATGTTGGCCGGATAGCGATTCGTCTAGGGTGGGCTCCCCTTGAACCATTGCCTGAACAAGTTCAACTACATGTATTAAAACAGATGCCACTGTTGGATACCATTCAGAAGTATCTTTGGCCACGGCTAAAAACCTTGGATCCAAAAACATTGTACGAACTACATTACCAAATGATAACCTTTGGAAAGGTCTTTTGTACAAAGCAAAAGCCAAATTGCAATTCTTGTCCAATGAAGGGAGAGTGCAGACATTACGCTAGTGCATTTGCAAGCGCCAGGCTTGCCTTACCAGGGCCAGAAAAGAAGCCAGGTTCAACAAAGAACGGTAGTTCTACCGATCCCTTGATGATGGCTCTTCAGGCACTCAATCCACCAACTGTCTCCTCTCTTGAGGTCAGCCTTGACTCAAAATACCAAAGCAAAAGTTGTGAACCTATTATTGAGGAACCATCATCACCGCAGCCTGAATATACAGAAACATCATTAAGAGACATTGAAGATCTTTTTCTTGATGACCCTAATGACATGCCAACAATTAAAATGAGAGATGAAAGATTTTCAACAATATTAGAGCCGTACACAGGTGTATTTCAAGAAAACGATATGGCAACAGGATTAGTTCCTTCCCGATATGCAAATTTTCAAGCTCCCAAATCGAAGGATGCTTTCCGCTTATGCACGAAGCACCTAGTCTATGTTCTTCCAGATTATCATCCTCTATTGCAGACTCTAGAAAACAGAGATCCCGATGATCCATGCCCATACCTTCTTGCTATATGGCCATCAGATGAAAAACGATGCGATCCTCAAGAGTCGAGAGAATTGAGCAATATTGTAGAGCCATCTTTTTCACTTAATAATCATTCACAAGCAGCTTCACAAATGGTTCGTGGAACACTTTTGATGCCGTGTCGAACTGCAACAAGGGGGAGCTTTCCACTCAACGGGACATACTTTCAAGTGAATGAGATATTTGCTGACTATGAAACCAGTATACGCCCCATTGAAGTTCCTAGATCAAGCTTATGGAGTCTAGCTAGGTTAACTGTATACTGTGGGACCTCAACATCTTCAATCCTTAGAGGCTTACAATTGAACGAAATTAAGGACTGCTTTGTGAAAGGTTTTATCTGTGTCCGAGCCTTCAACCGGAAAACACGCGCTCCTGAAGAACTTTCAGATAGATTCCACGTTCGACCAACTAAGAAAGGAGACAAGGGAGGCAAAGGAGGCAAGGGAGGCAAGAAGAACAAAAAAGAGGATGAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Perm-CXXC RRM_DME

Protein Analysis

1391

Amino Acids

155.54

Weight (kDa)

8.57

Isoelectric Point (pI)

53.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_DME PF15628 1265 - 1365 3.5e-46 RRM in Demeter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 797
AccB1I GGYRCC 1 cut(s) 109
AccB7I CCANNNNNTGG 1 cut(s) 887
AccI GTMKAC 4 cut(s) 18, 1364, 3895, 3951
AccII CGCG 1 cut(s) 4062
AciI CCGC 7 cut(s) 506, 803, 1345, 2678, 2792, 3359, 3571
AclWI GGATC 8 cut(s) 2061, 2760, 3035, 3048, 3233, 3638, 3647, 3698
AcoI YGGCCR 5 cut(s) 3, 2695, 2908, 3020, 3680
AcuI CTGAAG 3 cut(s) 261, 3242, 4089
AcyI GRCGYC 2 cut(s) 794, 1801
AfaI GTAC 7 cut(s) 339, 1692, 2745, 2813, 3056, 3100, 3479
AflIII ACRYGT 3 cut(s) 1741, 2878, 2971
AjnI CCWGG 5 cut(s) 1994, 2442, 3176, 3190, 3208
AjuI GAANNNNNNNTTGG 6 cut(s) 1313, 1345, 1529, 1561, 3106, 3138
AleI CACNNNNGTG 2 cut(s) 1602, 2217
Alw21I GWGCWC 1 cut(s) 1480
Alw26I GTCTC 6 cut(s) 39, 1392, 2854, 3286, 3384, 4107
Alw44I GTGCAC 1 cut(s) 1476
AlwI GGATC 8 cut(s) 2061, 2760, 3035, 3048, 3233, 3638, 3647, 3698
AlwNI CAGNNNCTG 1 cut(s) 1489
Ama87I CYCGRG 2 cut(s) 1527, 2771
ApaI GGGCCC 1 cut(s) 1640
ApaLI GTGCAC 1 cut(s) 1476
ArsI GACNNNNNNTTYG 2 cut(s) 2483, 2515
AseI ATTAAT 1 cut(s) 1941
Asp700I GAANNNNTTC 1 cut(s) 2961
AspLEI GCGC 3 cut(s) 1106, 3176, 4064
AspS9I GGNCC 6 cut(s) 908, 1117, 1636, 1637, 3194, 3960
AsuC2I CCSGG 2 cut(s) 1957, 2891
AvaI CYCGRG 2 cut(s) 1527, 2771
AvaII GGWCC 1 cut(s) 3960
AxyI CCTNAGG 1 cut(s) 2325
BaeGI GKGCMC 2 cut(s) 1480, 1640
BalI TGGCCA 3 cut(s) 2697, 3022, 3682
BamHI GGATCC 1 cut(s) 3040
BanI GGYRCC 1 cut(s) 109
BanII GRGCYC 4 cut(s) 848, 1640, 2384, 2938
BbsI GAAGAC 4 cut(s) 172, 970, 1086, 2561
Bbv12I GWGCWC 1 cut(s) 1480
BceAI ACGGC 3 cut(s) 3041, 3460, 3793
BciT130I CCWGG 5 cut(s) 1996, 2444, 3178, 3192, 3210
BciVI GTATCC 1 cut(s) 2992
BclI TGATCA 2 cut(s) 727, 2479
BcnI CCSGG 2 cut(s) 1957, 2891
BcoDI GTCTC 6 cut(s) 39, 1392, 2854, 3286, 3384, 4107
BfmI CTRYAG 6 cut(s) 135, 677, 975, 1481, 2242, 2547
BfoI RGCGCY 2 cut(s) 1107, 3177
BfuI GTATCC 1 cut(s) 2992
BglII AGATCT 2 cut(s) 1465, 3400
Bme1390I CCNGG 7 cut(s) 1957, 1996, 2444, 2891, 3178, 3192, 3210
Bme18I GGWCC 1 cut(s) 3960
BmeT110I CYCGRG 2 cut(s) 1527, 2771
BmgT120I GGNCC 6 cut(s) 908, 1117, 1636, 1637, 3194, 3960
BmrFI CCNGG 7 cut(s) 1957, 1996, 2444, 2891, 3178, 3192, 3210
BmrI ACTGGG 2 cut(s) 2378, 2647
BmsI GCATC 6 cut(s) 448, 1726, 2976, 3553, 3689, 3795
BmuI ACTGGG 2 cut(s) 2378, 2647
BpiI GAAGAC 4 cut(s) 172, 970, 1086, 2561
BplI GAGNNNNNCTC 8 cut(s) 424, 456, 835, 867, 1389, 1421, 2246, 2278
BpuEI CTTGAG 3 cut(s) 1406, 3311, 3693
BpuMI CCSGG 2 cut(s) 1957, 2891
BsaBI GATNNNNATC 1 cut(s) 3612
BsaHI GRCGYC 2 cut(s) 794, 1801
BsaI GGTCTC 1 cut(s) 1392
BsaWI WCCGGW 4 cut(s) 643, 722, 2467, 4050
BsaXI ACNNNNNCTCC 8 cut(s) 533, 563, 1533, 1563, 2503, 2533, 4048, 4078
Bse1I ACTGG 4 cut(s) 2384, 2598, 2642, 3891
Bse21I CCTNAGG 1 cut(s) 2325
Bse3DI GCAATG 4 cut(s) 670, 719, 1759, 2948
Bse8I GATNNNNATC 1 cut(s) 3612
BseBI CCWGG 5 cut(s) 1996, 2444, 3178, 3192, 3210
BseGI GGATG 9 cut(s) 463, 765, 766, 1618, 1965, 2902, 3568, 3613, 4168
BseJI GATNNNNATC 1 cut(s) 3612
BseMI GCAATG 4 cut(s) 670, 719, 1759, 2948
BseMII CTCAG 7 cut(s) 596, 866, 1288, 2190, 2316, 2367, 2810
BseNI ACTGG 4 cut(s) 2384, 2598, 2642, 3891
BseSI GKGCMC 2 cut(s) 1480, 1640
BseYI CCCAGC 1 cut(s) 1748
BsgI GTGCAG 1 cut(s) 3166
Bsh1236I CGCG 1 cut(s) 4062
BshNI GGYRCC 1 cut(s) 109
BsiHKAI GWGCWC 1 cut(s) 1480
BsiHKCI CYCGRG 2 cut(s) 1527, 2771
BsiSI CCGG 7 cut(s) 644, 723, 1957, 2468, 2891, 2911, 4051
BslFI GGGAC 8 cut(s) 201, 1446, 1533, 1545, 1939, 2092, 3862, 3973
BsmAI GTCTC 6 cut(s) 39, 1392, 2854, 3286, 3384, 4107
BsmFI GGGAC 8 cut(s) 201, 1446, 1533, 1545, 1939, 2092, 3862, 3973
BsmI GAATGC 2 cut(s) 1216, 1764
Bso31I GGTCTC 1 cut(s) 1392
BsoBI CYCGRG 2 cut(s) 1527, 2771
Bsp120I GGGCCC 1 cut(s) 1636
Bsp1286I GDGCHC 5 cut(s) 848, 1480, 1640, 2384, 2938
Bsp1407I TGTACA 2 cut(s) 1690, 3098
Bsp19I CCATGG 3 cut(s) 54, 1714, 1759
BspACI CCGC 7 cut(s) 506, 803, 1345, 2678, 2792, 3359, 3571
BspCNI CTCAG 7 cut(s) 595, 865, 1287, 2191, 2317, 2368, 2809
BspFNI CGCG 1 cut(s) 4062
BspMAI CTGCAG 2 cut(s) 681, 1485
BspPI GGATC 8 cut(s) 2061, 2760, 3035, 3048, 3233, 3638, 3647, 3698
BspQI GCTCTTC 1 cut(s) 3261
BspT107I GGYRCC 1 cut(s) 109
BspTNI GGTCTC 1 cut(s) 1392
BsrDI GCAATG 4 cut(s) 670, 719, 1759, 2948
BsrGI TGTACA 2 cut(s) 1690, 3098
BsrI ACTGG 4 cut(s) 2384, 2598, 2642, 3891
BssNAI GTATAC 3 cut(s) 19, 3896, 3952
BssNI GRCGYC 2 cut(s) 794, 1801
BssT1I CCWWGG 7 cut(s) 54, 968, 1134, 1714, 1759, 1972, 3036
Bst1107I GTATAC 3 cut(s) 19, 3896, 3952
Bst2UI CCWGG 5 cut(s) 1996, 2444, 3178, 3192, 3210
Bst4CI ACNGT 8 cut(s) 532, 632, 1793, 2994, 3227, 3280, 3949, 3956
Bst6I CTCTTC 3 cut(s) 492, 1890, 3261
BstACI GRCGYC 2 cut(s) 794, 1801
BstAPI GCANNNNNTGC 1 cut(s) 131
BstAUI TGTACA 2 cut(s) 1690, 3098
BstC8I GCNNGC 4 cut(s) 132, 463, 3172, 3183
BstDSI CCRYGG 4 cut(s) 54, 1714, 1759, 3023
BstEII GGTNACC 1 cut(s) 1181
BstENI CCTNNNNNAGG 1 cut(s) 135
BstF5I GGATG 9 cut(s) 463, 765, 766, 1618, 1965, 2902, 3568, 3613, 4168
BstFNI CGCG 1 cut(s) 4062
BstH2I RGCGCY 2 cut(s) 1107, 3177
BstHHI GCGC 3 cut(s) 1106, 3176, 4064
BstMAI GTCTC 6 cut(s) 39, 1392, 2854, 3286, 3384, 4107
BstMWI GCNNNNNNNGC 9 cut(s) 131, 550, 843, 979, 1807, 3182, 3260, 4131, 4140
BstNI CCWGG 5 cut(s) 1996, 2444, 3178, 3192, 3210
BstNSI RCATGY 7 cut(s) 1061, 1317, 1439, 1745, 2882, 2975, 3430
BstPI GGTNACC 1 cut(s) 1181
BstSCI CCNGG 7 cut(s) 1955, 1994, 2442, 2889, 3176, 3190, 3208
BstSFI CTRYAG 6 cut(s) 135, 677, 975, 1481, 2242, 2547
BstSLI GKGCMC 2 cut(s) 1480, 1640
BstUI CGCG 1 cut(s) 4062
BstV2I GAAGAC 4 cut(s) 172, 970, 1086, 2561
BstX2I RGATCY 4 cut(s) 1465, 3040, 3400, 3643
BstXI CCANNNNNNTGG 1 cut(s) 1114
BstYI RGATCY 4 cut(s) 1465, 3040, 3400, 3643
BstZ17I GTATAC 3 cut(s) 19, 3896, 3952
Bsu36I CCTNAGG 1 cut(s) 2325
BsuI GTATCC 1 cut(s) 2992
BtgI CCRYGG 4 cut(s) 54, 1714, 1759, 3023
BtsCI GGATG 9 cut(s) 463, 765, 766, 1618, 1965, 2902, 3568, 3613, 4168
BtsI GCAGTG 1 cut(s) 1478
BtsIMutI CAGTG 4 cut(s) 1478, 1487, 2353, 2990
Cac8I GCNNGC 4 cut(s) 132, 463, 3172, 3183
CaiI CAGNNNCTG 1 cut(s) 1489
CfoI GCGC 3 cut(s) 1106, 3176, 4064
Cfr13I GGNCC 6 cut(s) 908, 1117, 1636, 1637, 3194, 3960
CseI GACGC 2 cut(s) 1001, 1790
CsiI ACCWGGT 1 cut(s) 2442
Csp6I GTAC 7 cut(s) 338, 1691, 2744, 2812, 3055, 3099, 3478
CspCI CAANNNNNGTGG 2 cut(s) 1807, 1842
CviQI GTAC 7 cut(s) 338, 1691, 2744, 2812, 3055, 3099, 3478
DraI TTTAAA 1 cut(s) 2475
EaeI YGGCCR 5 cut(s) 3, 2695, 2908, 3020, 3680
Eam1104I CTCTTC 3 cut(s) 492, 1890, 3261
EarI CTCTTC 3 cut(s) 492, 1890, 3261
Eco130I CCWWGG 7 cut(s) 54, 968, 1134, 1714, 1759, 1972, 3036
Eco147I AGGCCT 1 cut(s) 973
Eco24I GRGCYC 4 cut(s) 848, 1640, 2384, 2938
Eco31I GGTCTC 1 cut(s) 1392
Eco32I GATATC 1 cut(s) 1708
Eco47I GGWCC 1 cut(s) 3960
Eco57I CTGAAG 3 cut(s) 261, 3242, 4089
Eco81I CCTNAGG 1 cut(s) 2325
Eco88I CYCGRG 2 cut(s) 1527, 2771
Eco91I GGTNACC 1 cut(s) 1181
EcoNI CCTNNNNNAGG 1 cut(s) 135
EcoO109I RGGNCCY 3 cut(s) 1636, 1637, 3960
EcoO65I GGTNACC 1 cut(s) 1181
EcoRII CCWGG 5 cut(s) 1994, 2442, 3176, 3190, 3208
EcoRV GATATC 1 cut(s) 1708
EcoT14I CCWWGG 7 cut(s) 54, 968, 1134, 1714, 1759, 1972, 3036
EcoT22I ATGCAT 1 cut(s) 1059
EcoT38I GRGCYC 4 cut(s) 848, 1640, 2384, 2938
ErhI CCWWGG 7 cut(s) 54, 968, 1134, 1714, 1759, 1972, 3036
FalI AAGNNNNNCTT 4 cut(s) 851, 883, 3998, 4030
FaqI GGGAC 8 cut(s) 201, 1446, 1533, 1545, 1939, 2092, 3862, 3973
FauI CCCGC 2 cut(s) 1338, 2799
FbaI TGATCA 2 cut(s) 727, 2479
FblI GTMKAC 4 cut(s) 18, 1364, 3895, 3951
FokI GGATG 8 cut(s) 470, 752, 773, 1605, 1972, 2909, 3575, 3600
FriOI GRGCYC 4 cut(s) 848, 1640, 2384, 2938
GlaI GCGC 3 cut(s) 1105, 3175, 4063
GsaI CCCAGC 1 cut(s) 1752
HaeII RGCGCY 2 cut(s) 1107, 3177
HapII CCGG 7 cut(s) 644, 723, 1957, 2468, 2891, 2911, 4051
HgaI GACGC 2 cut(s) 1001, 1790
HhaI GCGC 3 cut(s) 1106, 3176, 4064
Hin1I GRCGYC 2 cut(s) 794, 1801
Hin6I GCGC 3 cut(s) 1104, 3174, 4062
HinP1I GCGC 3 cut(s) 1104, 3174, 4062
HincII GTYRAC 4 cut(s) 1365, 1723, 2295, 3945
HindII GTYRAC 4 cut(s) 1365, 1723, 2295, 3945
HindIII AAGCTT 1 cut(s) 3922
HpaI GTTAAC 2 cut(s) 1723, 3945
HpaII CCGG 7 cut(s) 644, 723, 1957, 2468, 2891, 2911, 4051
Hpy99I CGWCG 1 cut(s) 2515
HpyCH4III ACNGT 8 cut(s) 532, 632, 1793, 2994, 3227, 3280, 3949, 3956
HpyCH4IV ACGT 2 cut(s) 794, 4092
HpyF10VI GCNNNNNNNGC 9 cut(s) 131, 550, 843, 979, 1807, 3182, 3260, 4131, 4140
HpySE526I ACGT 2 cut(s) 794, 4092
Hsp92I GRCGYC 2 cut(s) 794, 1801
HspAI GCGC 3 cut(s) 1104, 3174, 4062
Ksp22I TGATCA 2 cut(s) 727, 2479
KspAI GTTAAC 2 cut(s) 1723, 3945
LguI GCTCTTC 1 cut(s) 3261
LweI GCATC 6 cut(s) 448, 1726, 2976, 3553, 3689, 3795
MabI ACCWGGT 1 cut(s) 2442
MaeII ACGT 2 cut(s) 794, 4092
MaeIII GTNAC 9 cut(s) 1129, 1181, 1253, 1418, 1549, 2079, 2149, 2208, 2803
MfeI CAATTG 2 cut(s) 306, 3992
MflI RGATCY 4 cut(s) 1465, 3040, 3400, 3643
MhlI GDGCHC 5 cut(s) 848, 1480, 1640, 2384, 2938
MlsI TGGCCA 3 cut(s) 2697, 3022, 3682
MluNI TGGCCA 3 cut(s) 2697, 3022, 3682
MlyI GAGTC 7 cut(s) 1222, 2009, 2264, 3299, 3622, 3722, 3940
MmeI TCCRAC 7 cut(s) 390, 2005, 2031, 2139, 2146, 2899, 2976
Mox20I TGGCCA 3 cut(s) 2697, 3022, 3682
Mph1103I ATGCAT 1 cut(s) 1059
MroXI GAANNNNTTC 1 cut(s) 2961
MscI TGGCCA 3 cut(s) 2697, 3022, 3682
MslI CAYNNNNRTG 3 cut(s) 1602, 2141, 2217
Msp20I TGGCCA 3 cut(s) 2697, 3022, 3682
MspI CCGG 7 cut(s) 644, 723, 1957, 2468, 2891, 2911, 4051
MspR9I CCNGG 7 cut(s) 1957, 1996, 2444, 2891, 3178, 3192, 3210
MunI CAATTG 2 cut(s) 306, 3992
Mva1269I GAATGC 2 cut(s) 1216, 1764
MvaI CCWGG 5 cut(s) 1996, 2444, 3178, 3192, 3210
MvnI CGCG 1 cut(s) 4062
MwoI GCNNNNNNNGC 9 cut(s) 131, 550, 843, 979, 1807, 3182, 3260, 4131, 4140
NciI CCSGG 2 cut(s) 1957, 2891
NcoI CCATGG 3 cut(s) 54, 1714, 1759
NmeAIII GCCGAG 1 cut(s) 1111
NmuCI GTSAC 7 cut(s) 1129, 1181, 1253, 1418, 1549, 2079, 2149
NsiI ATGCAT 1 cut(s) 1059
NspI RCATGY 7 cut(s) 1061, 1317, 1439, 1745, 2882, 2975, 3430
OliI CACNNNNGTG 2 cut(s) 1602, 2217
PaeR7I CTCGAG 1 cut(s) 2771
PceI AGGCCT 1 cut(s) 973
PciI ACATGT 3 cut(s) 1741, 2878, 2971
PciSI GCTCTTC 1 cut(s) 3261
PctI GAATGC 2 cut(s) 1216, 1764
PdmI GAANNNNTTC 1 cut(s) 2961
PflMI CCANNNNNTGG 1 cut(s) 887
PfoI TCCNGGA 1 cut(s) 1955
PleI GAGTC 7 cut(s) 1222, 2009, 2263, 3299, 3622, 3721, 3939
PpsI GAGTC 7 cut(s) 1222, 2009, 2263, 3299, 3622, 3721, 3939
PpuMI RGGWCCY 1 cut(s) 3960
PscI ACATGT 3 cut(s) 1741, 2878, 2971
PshBI ATTAAT 1 cut(s) 1941
Psp5II RGGWCCY 1 cut(s) 3960
Psp6I CCWGG 5 cut(s) 1994, 2442, 3176, 3190, 3208
PspEI GGTNACC 1 cut(s) 1181
PspFI CCCAGC 1 cut(s) 1748
PspGI CCWGG 5 cut(s) 1994, 2442, 3176, 3190, 3208
PspOMI GGGCCC 1 cut(s) 1636
PspPI GGNCC 6 cut(s) 908, 1117, 1636, 1637, 3194, 3960
PspPPI RGGWCCY 1 cut(s) 3960
PspXI VCTCGAGB 1 cut(s) 2771
PstI CTGCAG 2 cut(s) 681, 1485
PstNI CAGNNNCTG 1 cut(s) 1489
PsuI RGATCY 4 cut(s) 1465, 3040, 3400, 3643
RsaI GTAC 7 cut(s) 339, 1692, 2745, 2813, 3056, 3100, 3479
RsaNI GTAC 7 cut(s) 338, 1691, 2744, 2812, 3055, 3099, 3478
RseI CAYNNNNRTG 3 cut(s) 1602, 2141, 2217
SalI GTCGAC 1 cut(s) 1363
SapI GCTCTTC 1 cut(s) 3261
Sau96I GGNCC 6 cut(s) 908, 1117, 1636, 1637, 3194, 3960
SchI GAGTC 7 cut(s) 1222, 2009, 2264, 3299, 3622, 3722, 3940
ScrFI CCNGG 7 cut(s) 1957, 1996, 2444, 2891, 3178, 3192, 3210
SduI GDGCHC 5 cut(s) 848, 1480, 1640, 2384, 2938
SexAI ACCWGGT 1 cut(s) 2442
SfaNI GCATC 6 cut(s) 448, 1726, 2976, 3553, 3689, 3795
SfcI CTRYAG 6 cut(s) 135, 677, 975, 1481, 2242, 2547
Sfr274I CTCGAG 1 cut(s) 2771
SinI GGWCC 1 cut(s) 3960
SlaI CTCGAG 1 cut(s) 2771
SmiI ATTTAAAT 1 cut(s) 2475
SmiMI CAYNNNNRTG 3 cut(s) 1602, 2141, 2217
SmlI CTYRAG 4 cut(s) 1385, 2771, 3290, 3708
SmoI CTYRAG 4 cut(s) 1385, 2771, 3290, 3708
SseBI AGGCCT 1 cut(s) 973
SsiI CCGC 7 cut(s) 506, 803, 1345, 2678, 2792, 3359, 3571
SspI AATATT 3 cut(s) 2176, 3468, 3733
StuI AGGCCT 1 cut(s) 973
StyD4I CCNGG 7 cut(s) 1955, 1994, 2442, 2889, 3176, 3190, 3208
StyI CCWWGG 7 cut(s) 54, 968, 1134, 1714, 1759, 1972, 3036
SwaI ATTTAAAT 1 cut(s) 2475
TaaI ACNGT 8 cut(s) 532, 632, 1793, 2994, 3227, 3280, 3949, 3956
TaiI ACGT 2 cut(s) 797, 4095
TatI WGTACW 3 cut(s) 1690, 2743, 3098
TauI GCSGC 3 cut(s) 509, 806, 2681
TscAI CASTG 4 cut(s) 1485, 1494, 2353, 2997
TseFI GTSAC 7 cut(s) 1129, 1181, 1253, 1418, 1549, 2079, 2149
Tsp45I GTSAC 7 cut(s) 1129, 1181, 1253, 1418, 1549, 2079, 2149
TspGWI ACGGA 5 cut(s) 1179, 1272, 1549, 1674, 1951
TspRI CASTG 4 cut(s) 1485, 1494, 2353, 2997
Van91I CCANNNNNTGG 1 cut(s) 887
VneI GTGCAC 1 cut(s) 1476
VpaK11BI GGWCC 1 cut(s) 3960
VspI ATTAAT 1 cut(s) 1941
XagI CCTNNNNNAGG 1 cut(s) 135
XbaI TCTAGA 2 cut(s) 1174, 3631
XceI RCATGY 7 cut(s) 1061, 1317, 1439, 1745, 2882, 2975, 3430
XcmI CCANNNNNNNNNTGG 1 cut(s) 2162
XhoI CTCGAG 1 cut(s) 2771
XmiI GTMKAC 4 cut(s) 18, 1364, 3895, 3951
XmnI GAANNNNTTC 1 cut(s) 2961
ZraI GACGTC 1 cut(s) 795
Zsp2I ATGCAT 1 cut(s) 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.