pycom11g17140

E3 ubiquitin ligase BIG BROTHER-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0010546
Physical Location & Seq
Reverse (-)
19165612 .. 19168807
3196 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 456 bp
ATGACTAATGAACAGAATGTGGCAGTGAACTCAGAATGGGAAGGAAATGCAAATACTTCTACACCCAACAACCCCATAGAATGTCCACGGAGCCATCAAAATTCCCATGATCATCAGGTTATTTGGCAAGACAACATCGATCCTGACAGCATGAGTTACGAGGAATTACTTGAGTTAGGTGAGGCAGTTGGAACTCAAAGTCGAGGTCTCTCCCAAGATCAGATCTCCTTGCTTCCAATCTCAAAGTACAAATGCAGCTTTTTCTTGAGAAAGAAATCACGAGACGAGAGATGTGTGATTTGCCAGATGGAATATAAACGAGGGGACCGGCGGATTGCTCTACCGTGCAAACACCTCTATCATGCTGGTTGTGGGACCAGATGGCTAAGCATCAACAAGGCTTGCCCAATGTGTTACACTGAGGTGTTTGGTGACGCGTCAAAGAGCGCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

152

Amino Acids

17.19

Weight (kDa)

6.51

Isoelectric Point (pI)

50.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 92 - 138 1.1e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 97 - 138 7.6e-13 Ring finger domain
zf-C3HC4_2 PF13923 97 - 138 1.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 98 - 124 1.7e-07 RING-like zinc finger
zf-C3HC4 PF00097 98 - 138 4.6e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 437
AciI CCGC 1 cut(s) 331
AclWI GGATC 1 cut(s) 134
AcsI RAATTY 1 cut(s) 100
AfaI GTAC 1 cut(s) 248
AflIII ACRYGT 1 cut(s) 435
AleI CACNNNNGTG 1 cut(s) 422
AluBI AGCT 1 cut(s) 258
AluI AGCT 1 cut(s) 258
Alw26I GTCTC 2 cut(s) 212, 276
AlwI GGATC 1 cut(s) 134
ApeKI GCWGC 1 cut(s) 255
ApoI RAATTY 1 cut(s) 100
ArsI GACNNNNNNTTYG 2 cut(s) 190, 222
AspLEI GCGC 1 cut(s) 449
AspS9I GGNCC 2 cut(s) 325, 375
AsuHPI GGTGA 2 cut(s) 191, 443
AvaII GGWCC 2 cut(s) 325, 375
BauI CACGAG 1 cut(s) 279
BbvI GCAGC 1 cut(s) 267
BccI CCATC 3 cut(s) 102, 301, 375
BclI TGATCA 1 cut(s) 109
BcoDI GTCTC 2 cut(s) 212, 276
BglII AGATCT 1 cut(s) 222
BisI GCNGC 1 cut(s) 256
BlpI GCTNAGC 1 cut(s) 386
BlsI GCNGC 1 cut(s) 257
Bme18I GGWCC 2 cut(s) 325, 375
BmgT120I GGNCC 2 cut(s) 325, 375
BmiI GGNNCC 3 cut(s) 92, 326, 376
BmsI GCATC 1 cut(s) 399
Bpu1102I GCTNAGC 1 cut(s) 386
BpuEI CTTGAG 2 cut(s) 191, 286
Bsa29I ATCGAT 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 1 cut(s) 86
Bse118I RCCGGY 1 cut(s) 327
BseCI ATCGAT 1 cut(s) 138
BseDI CCNNGG 1 cut(s) 86
BseMII CTCAG 2 cut(s) 45, 411
BseXI GCAGC 1 cut(s) 267
Bsh1236I CGCG 1 cut(s) 437
BshVI ATCGAT 1 cut(s) 138
BsiSI CCGG 1 cut(s) 328
BslFI GGGAC 2 cut(s) 338, 388
BsmAI GTCTC 2 cut(s) 212, 276
BsmBI CGTCTC 1 cut(s) 276
BsmFI GGGAC 2 cut(s) 338, 388
Bso31I GGTCTC 1 cut(s) 212
Bsp143I GATC 4 cut(s) 109, 139, 217, 222
Bsp1720I GCTNAGC 1 cut(s) 386
BspACI CCGC 1 cut(s) 331
BspCNI CTCAG 2 cut(s) 44, 412
BspDI ATCGAT 1 cut(s) 138
BspFNI CGCG 1 cut(s) 437
BspLI GGNNCC 3 cut(s) 92, 326, 376
BspPI GGATC 1 cut(s) 134
BspTNI GGTCTC 1 cut(s) 212
BsrFI RCCGGY 1 cut(s) 327
BssAI RCCGGY 1 cut(s) 327
BssECI CCNNGG 1 cut(s) 86
BssMI GATC 4 cut(s) 109, 139, 217, 222
BssSI CACGAG 1 cut(s) 279
Bst2BI CACGAG 1 cut(s) 279
Bst4CI ACNGT 1 cut(s) 345
BstC8I GCNNGC 1 cut(s) 403
BstDEI CTNAG 3 cut(s) 31, 386, 420
BstDSI CCRYGG 1 cut(s) 86
BstFNI CGCG 1 cut(s) 437
BstHHI GCGC 1 cut(s) 449
BstKTI GATC 4 cut(s) 112, 142, 220, 225
BstMAI GTCTC 2 cut(s) 212, 276
BstMBI GATC 4 cut(s) 109, 139, 217, 222
BstUI CGCG 1 cut(s) 437
BstV1I GCAGC 1 cut(s) 267
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
Bsu15I ATCGAT 1 cut(s) 138
BsuTUI ATCGAT 1 cut(s) 138
BtgI CCRYGG 1 cut(s) 86
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 2 cut(s) 30, 417
Cac8I GCNNGC 1 cut(s) 403
CfoI GCGC 1 cut(s) 449
Cfr10I RCCGGY 1 cut(s) 327
Cfr13I GGNCC 2 cut(s) 325, 375
ClaI ATCGAT 1 cut(s) 138
CseI GACGC 2 cut(s) 426, 443
Csp6I GTAC 1 cut(s) 247
CviAII CATG 3 cut(s) 107, 151, 362
CviJI RGCY 4 cut(s) 93, 258, 385, 401
CviKI_1 RGCY 4 cut(s) 93, 258, 385, 401
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 3 cut(s) 31, 386, 420
DpnI GATC 4 cut(s) 111, 141, 219, 224
DpnII GATC 4 cut(s) 109, 139, 217, 222
EciI GGCGGA 1 cut(s) 346
Eco31I GGTCTC 1 cut(s) 212
Eco47I GGWCC 2 cut(s) 325, 375
Esp3I CGTCTC 1 cut(s) 276
FaeI CATG 3 cut(s) 110, 154, 365
FaiI YATR 5 cut(s) 77, 108, 152, 315, 363
FaqI GGGAC 2 cut(s) 338, 388
FatI CATG 3 cut(s) 106, 150, 361
FbaI TGATCA 1 cut(s) 109
Fnu4HI GCNGC 1 cut(s) 256
Fsp4HI GCNGC 1 cut(s) 256
GlaI GCGC 1 cut(s) 448
GluI GCNGC 1 cut(s) 256
HapII CCGG 1 cut(s) 328
HgaI GACGC 2 cut(s) 426, 443
HhaI GCGC 1 cut(s) 449
Hin1II CATG 3 cut(s) 110, 154, 365
Hin6I GCGC 1 cut(s) 447
HinP1I GCGC 1 cut(s) 447
HpaII CCGG 1 cut(s) 328
HphI GGTGA 2 cut(s) 191, 443
Hpy166II GTNNAC 2 cut(s) 28, 86
Hpy188I TCNGA 2 cut(s) 34, 222
Hpy188III TCNNGA 3 cut(s) 143, 265, 279
Hpy8I GTNNAC 2 cut(s) 28, 86
HpyAV CCTTC 1 cut(s) 35
HpyCH4III ACNGT 1 cut(s) 345
HpyCH4V TGCA 3 cut(s) 50, 255, 348
HpyF3I CTNAG 3 cut(s) 31, 386, 420
Hsp92II CATG 3 cut(s) 110, 154, 365
HspAI GCGC 1 cut(s) 447
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 4 cut(s) 109, 139, 217, 222
LmnI GCTCC 1 cut(s) 90
LpnPI CCDG 6 cut(s) 101, 156, 317, 341, 351, 391
Lsp1109I GCAGC 1 cut(s) 267
LweI GCATC 1 cut(s) 399
MaeIII GTNAC 3 cut(s) 155, 413, 431
MalI GATC 4 cut(s) 111, 141, 219, 224
MboI GATC 4 cut(s) 109, 139, 217, 222
MflI RGATCY 1 cut(s) 222
MluCI AATT 2 cut(s) 100, 164
MluI ACGCGT 1 cut(s) 435
MmeI TCCRAC 1 cut(s) 169
MnlI CCTC 6 cut(s) 154, 175, 197, 314, 365, 415
MslI CAYNNNNRTG 1 cut(s) 422
MspI CCGG 1 cut(s) 328
MvnI CGCG 1 cut(s) 437
NdeII GATC 4 cut(s) 109, 139, 217, 222
NlaIII CATG 3 cut(s) 110, 154, 365
NlaIV GGNNCC 3 cut(s) 92, 326, 376
NmuCI GTSAC 1 cut(s) 431
OliI CACNNNNGTG 1 cut(s) 422
PkrI GCNGC 1 cut(s) 257
PspN4I GGNNCC 3 cut(s) 92, 326, 376
PspPI GGNCC 2 cut(s) 325, 375
PsuI RGATCY 1 cut(s) 222
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
RseI CAYNNNNRTG 1 cut(s) 422
SatI GCNGC 1 cut(s) 256
Sau3AI GATC 4 cut(s) 109, 139, 217, 222
Sau96I GGNCC 2 cut(s) 325, 375
SetI ASST 6 cut(s) 120, 181, 208, 260, 357, 426
SfaNI GCATC 1 cut(s) 399
SinI GGWCC 2 cut(s) 325, 375
SmiMI CAYNNNNRTG 1 cut(s) 422
SmlI CTYRAG 2 cut(s) 170, 265
SmoI CTYRAG 2 cut(s) 170, 265
Sse9I AATT 2 cut(s) 100, 164
SsiI CCGC 1 cut(s) 331
TaaI ACNGT 1 cut(s) 345
TaqI TCGA 2 cut(s) 138, 202
TasI AATT 2 cut(s) 100, 164
TatI WGTACW 1 cut(s) 246
TscAI CASTG 2 cut(s) 30, 424
TseFI GTSAC 1 cut(s) 431
TseI GCWGC 1 cut(s) 255
Tsp45I GTSAC 1 cut(s) 431
TspDTI ATGAA 1 cut(s) 24
TspGWI ACGGA 1 cut(s) 103
TspRI CASTG 2 cut(s) 30, 424
VpaK11BI GGWCC 2 cut(s) 325, 375
XapI RAATTY 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.