pycom12g00210

HEAT repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002980
Physical Location & Seq
Forward (+)
278138 .. 296541
18404 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5904 bp
ATGGCGAAGTATGTGACAACAGAGAACGCCCCCCTCTCTCAGTTCGGTGTACTGGTAGCGCAATTGGAGTCCATAGTCGCCTCAGCATCCCATAAGCCTCCGGAGCCACTTCTATGCTTCGATCTCCTCTCCGATCTCATCTCCGCCATTAACGAAGAGCCCAAGGAATCCATTTTGTTGTGGCAAAGGAGATGCGAGGACGCATTATATTCCTTGCTTATTCTTGGTGCTCGGCGACCTGTCCGCCATCTGGCTTCAGTTGCTATGGCGAGGGTTATATCTAAGGGAGATGGCATTTCTATATATTCTAGAGCGAGCAGTCTCCAAGGCTTTCTCTCTGATGGGAGAAGGAGTGAACCGCAAAAACTTGCTGGTGCTGCACAATGCTTAGGAGAATTATATAGACATTTTGGGAGGCGAATCACATCGGGTTTACTTGAAACGACTATCATTGCCATGAAACTTTTCAAGTTTAACGAGGAATTTGTGAGACAAGAAGCTTTATATATGCTTCAAAATGCTTTGGAAGGCTCTGGTGGAAACGCAGCGGCTTCAGCATATACAGAGGCATTTCGTCTCATCATGCGGTTTGCTGTTGGTGATAAATCATTTCTTGTTAGAATTGCTGCTGCCAGATGTCTGAAGGCATTTGCCATTATCGGAGGACCGGGTCTAGGGGTGGGAGAACTAGACAGTTCAGCTTCTTATTGTGTCAAGGCCCTTGAAGATCCTGTTTCATCTGTCCGTGATGCATTTGCTGAAGCATTGGGTTCGTTGCTTGCTCTTGGAATGAATCCTCATGCACAGGTTCAACCAAGGGGGAAAGGACCTTTTCCTCCAGCAAAGAAACTGGAAAGTGGTTTGCATAGGCATTTAGCTTTGCCTTTCAAAAAAGTTGGAGCTCGGTCAAAGGACGTGCGAGTTGGCATAACCTTGTCTTGGGTATTCTTTTTACAGGCTATTCGTCTAAAATACATGCATCCAGATAGTGAGCTACAAAACTATGCAATTCAGGTTATGGAAATGCTTTGTTCCGATAATTCTGTTGATGCCTATTCACTGCTTATGTCTCTAGATGCCAGTCCTTCCATGAAAATTGCTGCCCTACGTACTGCATCATATACCCTGAAAACGCTGGGAGAGGTTCCTGTTGAATTCAAGGAAGTTCTTGACGACACTGTTGTTGCAGCAGTATCTCATTCTTCACAACTTGTTCGAATTGAGGCTGCTTTGACATTACGTGCACTGGCTGAAGTTGATCCCACCTGTGTTGGTGGGTTAATTTCTTATGGAGTGACCATGCTGAATGCTTTGAGAGAAAATTTGGCATTTGAGAAGGGGAGCACTTTGCAATTAGAGCTTGATTCTTTGCATGGACAAGCTACTGTCTTGGCAGCTCTGGTGTCCATTTCCCCAAAATTACCTCTCGGTTTTCCTGCCAGATTGCCCAGGTCAATCCTTGAAGTTTCAAAGAAGATGCTGATTGAATCTAGTCGGAACCCTCTGGCAGCTACAATTGAAAAAGAAGCTGGGTGGTTACTTTTGTCATCCCTGTTAGCTTCTATGCCAAAGGAGGAACTTGAAGACCAGGTTTTTGATATTCTTTCCTTGTGGGCTTCCCTCTTTACTGGAAATCCAGAAGATGAAACTAACCATACTGGAGATTTGATAAGTAGGATTCGCATGTGGTCTGCTGCAGTTGATGCACTTACTGCATTTTTAAGATGCTTTCTTTCTCCTAATGATGGGAATAATGGGATTTTGCTTCAACCTGTACTTGTATACCTGAGTAGGGCTTTATCGTATATATCACTGATAGCAGCCAAGCAACTGCCAAATGTGAAGCCTGCTTTGGATATATTTATCGTCAGGACATTAATAGCTTACCAGTCTCTGATCGATCCAACAGCATATAAGAATGACCACCGCATGCTGGAACCAGTAAACAAGACATTGGTGAACCAGATGCTCCTCTGCTTTGGCATCTTGTTTGCCTCGCAGGATAGTGGTGGGATGCTGTCACTTCTTGGAACGATTGAGCAGTGCCTGAAAGCTGGCAAAAAGCAACCATGGCATGCAGGGAGCATAACTAATATATGTGTGGGGTTACTTTCAGGATTTAAGGCTTTGCTTTCCTTGCGTCCCCAACCATTAGCACTTGAGATACTAAACTCAGCACAGGCTATATTTCAGAGCATTCTGGCAGAGGGAGATATCTGTCCATCACAGCGTAGGGCTTCATCAGAATGTCTGGGTCTTTTAGCTCGTCTTGGAAATGATATCTTTACAGCAAGAATGACTAGATCTCTGCTTGGTGACTTAACTGGGGCAACTGATTCAAACTATGCTGGATCAATTGCTTTTGCTCTTGGCTGCATTCATCGCAGTGCAGGAGGCATGGCATTGTCAACTTTAGTGCCTTCAACTGTGAGCTCAATCTCGCTGCTTTCTAAAAGTTCAATAGCTGGTTTACAGATCTGGTCTTTGCATGGGCTTCTGTTAACCATTGAAGCTGCTGGCTTGTCCTATGTATCTCAAGTCCAGGCAACACTTGGTCTTGCTCTGGACATTCTTTTGTCTGAGGAGAATGGATGGGTTGCACTTCAGCAAGGTGTTGGCCGTCTTATAAATGCTATTGTTGCTGTTCTTGGTCCTGAACTTGCCCCTGGAAGTATTTTCTTTTCACGTTGCAAGTCTGTTGTTTCCGAGATAAGCTCTGGGCAAGAAACAGCAATGATGCTTGAGAGTGTACGCTTCACCCAGCAACTTGTTCTTTTTGCACCACAAGCTGTTTCCGTGCACACCCATGTGCAAACTCTTCTACCTACTTTGTCCTCAAGACAGGTTTCCATTGTTGTCGAGCAAATAGAAGAGAAGTTATTTCACATGCTGGATGAAGAAACAGATTCCGAGATAGGGGATCTGGTGCGCACCACAATCATGCGATTGCTCTATGCATCATGCCCTTCCTGTCCATCCCATTGGATATCAATTTGTCGTAATGTGATTCTTGCCACATCAATGCGAAGAAATGCTAATAGTAGTAGCAGCTTAGAAAATTATCCCTCAAAGGGTACAGAGGGTGACCCAAGTGTGAATTTTGGAGAAGACGATGAGAACATGGTTTCTAGCACCATAGGCCCCCATGGTATTCTGAATAGAGATAAACACCTCAGATACCGAACCAGAGTTTTTGCTGCTGAGTGTTTGAGTTACCTACCTAGAGCTGTTGGAAAGAATCGAGCTCATTTTGATCTTTGTACTGCTAGGAGCCAACCTACTAATGGACAGGCCTCTAGTGATTGGCTCGTCCTCCATATACAAGAACTTATAGCCCTTGCATACCAGTTAGAAAACTTGCAGCCAATTGGTGTGGGACTTCTGGGTACCATTACAGATAAGTTTGAAAAGATACCTGACCCGGAGCTTCCAGGGCACCTCCTACTGGAACAGTATCAGGCCCAGTTAGTATCTGCTGTCCGCACTGCCTTAGACTCGTCCTCTGGCCCTATTCTATTGGAAGCAGGCTTCCTGCTCGCTACAAAGATCAAGATAAGACTTCTTGCTGCTCATGCCTCTCTCAAATGTTATACATATGCGTTCTTGAGGAGACACCATAGTGGGGTTCCAGATGAATATCTAGCATTGTTACCATTATTTTCAAAGAGTTCAAGTATGCTTGGGAAGTACTGGATTAGTGTTTTGAAGGATTACAGTTATGTATTCTTGTGCTTGCATCTCAAGACAAAGTGGAATCCATTTCTTGATGGAATTCAGTCACCTCTGGTATCATCAAAGTTGCAACCTTGCTTAGAAGAATCATGGCCAGTAATATTGCAGGCAATTGCATTGGATGCAGTTCCTGTGAATATTGAAGAGGAGGAGTATTCAAACTCCACTATTCAAAATAAATCAAGAAATAGCTTATTGTCAGGACATCGCATGGTTGAAATGGAATCCGAAGAATATCAGTTTCTTTGGGGATTTGCTCTACTGGTCTTATTTCAGGGCCAATATTCTACCCTTGGTGAACTGAAAAGTCCAATTTATTTCATTAAAGCTAGTAGCGGTGAAGATTCAGCTAGTGAAGAATTATCTTTCCCAGGAATTAAGTTATATGAAATTGCATTACCGGTTTTCCAGTTTCTGGCGACCAAAAGGTTTGCCAGTGCAGGATTTCTCACTGTGGACATATGCAGAGAACTGCTACAGGTTTTCTCATATTCTATGTGCATGGATAATTCTTGGGATAGTCTTTCAGTGTCTGTTATATCACAGATTGTGAAGAACTGCCCTGAATCTTTTTATGAAGTGGACAACTTTGCGTATTTGGCAATGGAACTTTGTTTGGCTTATCTTTACAAAGTATTTCAAAGTAGTAAGGCAATATCTTTGGACAAACCCTCGGAGGATTTGATATCCACATTATTTATCACTGCTAAAACACTCGTAAACTGTTTTCAACCAAAGACACAGCTAATCTCTGCTGCATTGGCGTTTCTTTTAATTGGTTACAAGGGAATCAGAGAAGCCTCAACTGAGTTTTGTTTCTCCAAGGTGGACGATTTTTTCAAGTGCACAAGTCTATTATTGAAAAGCTTTATTGATGATAAATCCGGTGTCAGTGAAGATGGTCTTCTTCATATGCAAAAATTATTGGGAACTTGTCTGAGTGTGATAACTGATTTGACCGAGGATTGCATTAAGTCCATTCATCTGCAGGAAAATAAGAGATCTGACTTGCACATACTACTGCAAACAAAGCTTGCTTTCTCTCTTGAACAAACTATATCATTTGCCAAGTTGGGTTACGAAATAAATTATCCTGAAGAGAATAGAGATGGTGATTCTGTCTATTATACTATGTTTAAATACTGCACAAAATGTGTCCAAACTGTACTTACCGATTCAAATATACAGGTTCAATCAATTGGTTTACAAGTGCTAAAAAGTTTGGTACAGAAAACCCCAAATGTGGAAGGCAACAATTTTTTAATGCTCTTTGTTGGGGAGCTTACTGCAGATTTTTTTTTGATAATCCAGAATGCTTTAAAGAAACCTGTAACTGAAAAATCAGCAACTGTTGCTGGCGAATGCTTAAGACTCTTAGTAGTGCTGCAGACACTTTCAAAACCTAGTGAATGTCAGAGAGGTTTTATGAATTTACTCCTGGAAGCCGTAATCGTGGTTTTCAAGGCTTCAGAAGAAGGTTCTTCACTGGAAGTCGATACATTAAGAAGCACTGCTGTAAGGCTTGTTTCTCATCTTGCTCAGCTTCCTTCGTCAGCAGTTCACTTCAAGGATATATTGCTGTCTATGCCTGTAACACAACGACAGCAACTTCAGGGGTTCATCCGTGCGTCTGTGACACAAGAGCATAATGCAACCCAGCTGAAGCCCACAACTCCGTCATTAGAGATAAAATTACCAGTTCCAACAGAGGCAAGCAAAGAGAAACCTCCACCATCAGCCACCACAGCGCATTCTCTTTCTGATGACCAAGAAATCGAAGAGGCAGAAGAGGATGAAGATGATTGGGATGCTTTCCAATCTTTTCCTGCCACTTTAAAAGCAGCTGAAAGTGAATCAAAAGGAGAAAGCACAGCGGAAGAACCTGACTTGGAAGTGAGTACACAGAGTGATTACACTCATGGAGATTCTATTTCGCAACCTATCAACAACGTAGATGCAGTCGACGATACAGACCATCAAGAGGCTGGTGAAAGGGAGGTGATATCAGATGCCCCTGATGGTCTAAAATCCCCACAGGGTAACGCACCAACCAACGGTACTGAGGCAGAAGAACCCCATGATCGATCCAAACTAAGAGCGGTGTTAGTGGACCATGCGATGATCAGCAATTGGAGAGAGACGAGGAAGTGGTTTCCAGACAGGTGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1968

Amino Acids

215.87

Weight (kDa)

5.46

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Laa1_Sip1_HTR5 PF20210 1102 - 1175 2.3e-18 Laa1/Sip1/HEATR5 HEAT repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2627
Acc16I TGCGCA 1 cut(s) 2930
Acc65I GGTACC 1 cut(s) 3386
AccB1I GGYRCC 2 cut(s) 3386, 3435
AccB7I CCANNNNNTGG 1 cut(s) 4147
AccBSI CCGCTC 1 cut(s) 5831
AccI GTMKAC 2 cut(s) 1782, 5694
AccIII TCCGGA 1 cut(s) 100
AclWI GGATC 6 cut(s) 722, 1253, 1895, 2359, 2928, 5811
AcoI YGGCCR 2 cut(s) 2617, 3824
AcsI RAATTY 6 cut(s) 482, 1154, 1321, 3097, 3771, 5161
AdeI CACNNNGTG 1 cut(s) 5639
AflII CTTAAG 1 cut(s) 5098
AgeI ACCGGT 1 cut(s) 4132
AjiI CACGTC 1 cut(s) 916
AjnI CCWGG 7 cut(s) 1448, 1587, 2541, 2665, 3430, 4102, 5169
AjuI GAANNNNNNNTTGG 2 cut(s) 1835, 1867
AleI CACNNNNGTG 2 cut(s) 2807, 3618
Alw21I GWGCWC 8 cut(s) 232, 904, 1246, 1346, 2435, 2802, 3247, 4580
Alw26I GTCTC 7 cut(s) 326, 484, 581, 1074, 1896, 3604, 5864
Alw44I GTGCAC 3 cut(s) 1242, 2798, 4576
AlwI GGATC 6 cut(s) 722, 1253, 1895, 2359, 2928, 5811
AlwNI CAGNNNCTG 6 cut(s) 1894, 2047, 3863, 4147, 4265, 5081
Aor13HI TCCGGA 1 cut(s) 100
AoxI GGCC 8 cut(s) 717, 2617, 3139, 3291, 3459, 3505, 3824, 4009
ApaLI GTGCAC 3 cut(s) 1242, 2798, 4576
ApoI RAATTY 6 cut(s) 482, 1154, 1321, 3097, 3771, 5161
ArsI GACNNNNNNTTYG 2 cut(s) 1577, 1609
AseI ATTAAT 1 cut(s) 1877
AsiGI ACCGGT 1 cut(s) 4132
Asp700I GAANNNNTTC 1 cut(s) 464
Asp718I GGTACC 1 cut(s) 3386
AspLEI GCGC 3 cut(s) 61, 2931, 5482
AspS9I GGNCC 9 cut(s) 665, 718, 827, 2651, 3140, 3460, 3506, 4009, 5842
AsuC2I CCSGG 2 cut(s) 669, 3422
AsuII TTCGAA 1 cut(s) 1216
AvaII GGWCC 4 cut(s) 665, 827, 2651, 5842
BaeGI GKGCMC 4 cut(s) 1246, 2802, 3438, 4580
BalI TGGCCA 1 cut(s) 3826
BanI GGYRCC 2 cut(s) 3386, 3435
BanII GRGCYC 4 cut(s) 162, 904, 2435, 3247
BbsI GAAGAC 3 cut(s) 1590, 3114, 4628
Bbv12I GWGCWC 8 cut(s) 232, 904, 1246, 1346, 2435, 2802, 3247, 4580
BbvCI CCTCAGC 1 cut(s) 82
BceAI ACGGC 2 cut(s) 2604, 5162
BcgI CGANNNNNNTGC 2 cut(s) 753, 787
BciT130I CCWGG 7 cut(s) 1450, 1589, 2543, 2667, 3432, 4104, 5171
BclI TGATCA 1 cut(s) 5853
BcnI CCSGG 2 cut(s) 669, 3422
BcoDI GTCTC 7 cut(s) 326, 484, 581, 1074, 1896, 3604, 5864
BfmI CTRYAG 5 cut(s) 1695, 4208, 4718, 5019, 5117
BfrI CTTAAG 1 cut(s) 5098
BglII AGATCT 3 cut(s) 2303, 2475, 4733
BlpI GCTNAGC 1 cut(s) 5271
BmcAI AGTACT 1 cut(s) 3689
Bme1390I CCNGG 9 cut(s) 669, 1450, 1589, 2543, 2667, 3422, 3432, 4104, 5171
Bme18I GGWCC 4 cut(s) 665, 827, 2651, 5842
BmgBI CACGTC 1 cut(s) 916
BmgT120I GGNCC 9 cut(s) 665, 718, 827, 2651, 3140, 3460, 3506, 4009, 5842
BmiI GGNNCC 9 cut(s) 105, 1146, 1499, 1938, 3142, 3272, 3388, 3437, 3627
BmrFI CCNGG 9 cut(s) 669, 1450, 1589, 2543, 2667, 3422, 3432, 4104, 5171
BmrI ACTGGG 2 cut(s) 2334, 3457
BmuI ACTGGG 2 cut(s) 2334, 3457
BpiI GAAGAC 3 cut(s) 1590, 3114, 4628
BplI GAGNNNNNCTC 2 cut(s) 2699, 2731
BpmI CTGGAG 2 cut(s) 822, 1680
Bpu10I CCTNAGC 2 cut(s) 82, 388
Bpu1102I GCTNAGC 1 cut(s) 5271
Bpu14I TTCGAA 1 cut(s) 1216
BpuEI CTTGAG 6 cut(s) 2180, 2520, 2762, 2821, 3625, 3725
BpuMI CCSGG 2 cut(s) 669, 3422
Bsa29I ATCGAT 2 cut(s) 1899, 5815
BsaAI YACGTR 2 cut(s) 1109, 1241
BsaBI GATNNNNATC 1 cut(s) 3636
BsaWI WCCGGW 3 cut(s) 100, 4132, 4616
BsaXI ACNNNNNCTCC 2 cut(s) 337, 367
Bse118I RCCGGY 1 cut(s) 4132
Bse3DI GCAATG 3 cut(s) 450, 2739, 4341
Bse8I GATNNNNATC 1 cut(s) 3636
BseAI TCCGGA 1 cut(s) 100
BseBI CCWGG 7 cut(s) 1450, 1589, 2543, 2667, 3432, 4104, 5171
BseCI ATCGAT 2 cut(s) 1899, 5815
BseJI GATNNNNATC 1 cut(s) 3636
BseMI GCAATG 3 cut(s) 450, 2739, 4341
BseRI GAGGAG 6 cut(s) 116, 1961, 2597, 3622, 3893, 3896
BseSI GKGCMC 4 cut(s) 1246, 2802, 3438, 4580
BseYI CCCAGC 4 cut(s) 1135, 1529, 2760, 5388
BsgI GTGCAG 4 cut(s) 363, 2409, 4191, 4861
BshFI GGCC 8 cut(s) 719, 2619, 3141, 3293, 3461, 3507, 3826, 4011
BshNI GGYRCC 2 cut(s) 3386, 3435
BshTI ACCGGT 1 cut(s) 4132
BshVI ATCGAT 2 cut(s) 1899, 5815
BsiHKAI GWGCWC 8 cut(s) 232, 904, 1246, 1346, 2435, 2802, 3247, 4580
BsiSI CCGG 5 cut(s) 101, 668, 3422, 4133, 4617
BslFI GGGAC 2 cut(s) 2127, 3390
BsmAI GTCTC 7 cut(s) 326, 484, 581, 1074, 1896, 3604, 5864
BsmBI CGTCTC 2 cut(s) 581, 5864
BsmFI GGGAC 2 cut(s) 2127, 3390
BsmI GAATGC 6 cut(s) 1312, 2196, 2376, 5050, 5099, 5482
BsnI GGCC 8 cut(s) 719, 2619, 3141, 3293, 3461, 3507, 3826, 4011
Bsp119I TTCGAA 1 cut(s) 1216
Bsp13I TCCGGA 1 cut(s) 100
Bsp1720I GCTNAGC 1 cut(s) 5271
Bsp19I CCATGG 2 cut(s) 2069, 3145
BspANI GGCC 8 cut(s) 719, 2619, 3141, 3293, 3461, 3507, 3826, 4011
BspDI ATCGAT 2 cut(s) 1899, 5815
BspEI TCCGGA 1 cut(s) 100
BspLI GGNNCC 9 cut(s) 105, 1146, 1499, 1938, 3142, 3272, 3388, 3437, 3627
BspMAI CTGCAG 4 cut(s) 1699, 4722, 5023, 5121
BspPI GGATC 6 cut(s) 722, 1253, 1895, 2359, 2928, 5811
BspQI GCTCTTC 1 cut(s) 150
BspT104I TTCGAA 1 cut(s) 1216
BspT107I GGYRCC 2 cut(s) 3386, 3435
BspTI CTTAAG 1 cut(s) 5098
BsrBI CCGCTC 1 cut(s) 5831
BsrDI GCAATG 3 cut(s) 450, 2739, 4341
BsrFI RCCGGY 1 cut(s) 4132
BssAI RCCGGY 1 cut(s) 4132
BssNAI GTATAC 1 cut(s) 1783
BssT1I CCWWGG 7 cut(s) 162, 325, 815, 2069, 3145, 4024, 4554
Bst1107I GTATAC 1 cut(s) 1783
Bst2UI CCWGG 7 cut(s) 1450, 1589, 2543, 2667, 3432, 4104, 5171
Bst6I CTCTTC 7 cut(s) 150, 2823, 2865, 3870, 4824, 5505, 5514
BstAFI CTTAAG 1 cut(s) 5098
BstAPI GCANNNNNTGC 4 cut(s) 1703, 1712, 3854, 5084
BstBAI YACGTR 2 cut(s) 1109, 1241
BstBI TTCGAA 1 cut(s) 1216
BstDSI CCRYGG 2 cut(s) 2069, 3145
BstEII GGTNACC 1 cut(s) 3083
BstHHI GCGC 3 cut(s) 61, 2931, 5482
BstMAI GTCTC 7 cut(s) 326, 484, 581, 1074, 1896, 3604, 5864
BstNI CCWGG 7 cut(s) 1450, 1589, 2543, 2667, 3432, 4104, 5171
BstNSI RCATGY 5 cut(s) 979, 1687, 1933, 2078, 2890
BstPI GGTNACC 1 cut(s) 3083
BstSCI CCNGG 9 cut(s) 667, 1448, 1587, 2541, 2665, 3420, 3430, 4102, 5169
BstSFI CTRYAG 5 cut(s) 1695, 4208, 4718, 5019, 5117
BstSLI GKGCMC 4 cut(s) 1246, 2802, 3438, 4580
BstSNI TACGTA 1 cut(s) 1109
BstV2I GAAGAC 3 cut(s) 1590, 3114, 4628
BstX2I RGATCY 5 cut(s) 727, 2303, 2475, 2920, 4733
BstXI CCANNNNNNTGG 2 cut(s) 2982, 5895
BstYI RGATCY 5 cut(s) 727, 2303, 2475, 2920, 4733
BstZ17I GTATAC 1 cut(s) 1783
Bsu15I ATCGAT 2 cut(s) 1899, 5815
BsuRI GGCC 8 cut(s) 719, 2619, 3141, 3293, 3461, 3507, 3826, 4011
BsuTUI ATCGAT 2 cut(s) 1899, 5815
BtgI CCRYGG 2 cut(s) 2069, 3145
BtgZI GCGATG 3 cut(s) 2366, 3923, 5864
BtrI CACGTC 1 cut(s) 916
BtsI GCAGTG 6 cut(s) 1058, 2048, 2392, 3483, 4434, 5241
CaiI CAGNNNCTG 6 cut(s) 1894, 2047, 3863, 4147, 4265, 5081
CfoI GCGC 3 cut(s) 61, 2931, 5482
Cfr10I RCCGGY 1 cut(s) 4132
Cfr13I GGNCC 9 cut(s) 665, 718, 827, 2651, 3140, 3460, 3506, 4009, 5842
ClaI ATCGAT 2 cut(s) 1899, 5815
CseI GACGC 3 cut(s) 209, 2129, 5349
CsiI ACCWGGT 1 cut(s) 1587
CspAI ACCGGT 1 cut(s) 4132
CspCI CAANNNNNGTGG 4 cut(s) 1252, 1287, 3354, 3389
DraI TTTAAA 3 cut(s) 4870, 5052, 5568
DraIII CACNNNGTG 1 cut(s) 5639
EaeI YGGCCR 2 cut(s) 2617, 3824
Eam1104I CTCTTC 7 cut(s) 150, 2823, 2865, 3870, 4824, 5505, 5514
EarI CTCTTC 7 cut(s) 150, 2823, 2865, 3870, 4824, 5505, 5514
EciI GGCGGA 2 cut(s) 133, 233
Ecl136II GAGCTC 3 cut(s) 902, 2433, 3245
Eco105I TACGTA 1 cut(s) 1109
Eco130I CCWWGG 7 cut(s) 162, 325, 815, 2069, 3145, 4024, 4554
Eco147I AGGCCT 1 cut(s) 3293
Eco24I GRGCYC 4 cut(s) 162, 904, 2435, 3247
Eco32I GATATC 5 cut(s) 2215, 2281, 2988, 4419, 5736
Eco47I GGWCC 4 cut(s) 665, 827, 2651, 5842
Eco53kI GAGCTC 3 cut(s) 902, 2433, 3245
Eco91I GGTNACC 1 cut(s) 3083
EcoICRI GAGCTC 3 cut(s) 902, 2433, 3245
EcoO109I RGGNCCY 3 cut(s) 718, 827, 3140
EcoO65I GGTNACC 1 cut(s) 3083
EcoRI GAATTC 2 cut(s) 1154, 3771
EcoRII CCWGG 7 cut(s) 1448, 1587, 2541, 2665, 3430, 4102, 5169
EcoRV GATATC 5 cut(s) 2215, 2281, 2988, 4419, 5736
EcoT14I CCWWGG 7 cut(s) 162, 325, 815, 2069, 3145, 4024, 4554
EcoT22I ATGCAT 3 cut(s) 754, 981, 2959
EcoT38I GRGCYC 4 cut(s) 162, 904, 2435, 3247
ErhI CCWWGG 7 cut(s) 162, 325, 815, 2069, 3145, 4024, 4554
Esp3I CGTCTC 2 cut(s) 581, 5864
FalI AAGNNNNNCTT 2 cut(s) 4620, 4652
FaqI GGGAC 2 cut(s) 2127, 3390
FauNDI CATATG 3 cut(s) 3595, 4193, 4644
FbaI TGATCA 1 cut(s) 5853
FblI GTMKAC 2 cut(s) 1782, 5694
FriOI GRGCYC 4 cut(s) 162, 904, 2435, 3247
FspAI RTGCGCAY 1 cut(s) 2930
FspI TGCGCA 1 cut(s) 2930
GlaI GCGC 3 cut(s) 60, 2930, 5481
GsaI CCCAGC 4 cut(s) 1139, 1533, 2764, 5392
GsuI CTGGAG 2 cut(s) 822, 1680
HaeIII GGCC 8 cut(s) 719, 2619, 3141, 3293, 3461, 3507, 3826, 4011
HapII CCGG 5 cut(s) 101, 668, 3422, 4133, 4617
HgaI GACGC 3 cut(s) 209, 2129, 5349
HhaI GCGC 3 cut(s) 61, 2931, 5482
Hin6I GCGC 3 cut(s) 59, 2929, 5480
HinP1I GCGC 3 cut(s) 59, 2929, 5480
HincII GTYRAC 3 cut(s) 2409, 2502, 5695
HindII GTYRAC 3 cut(s) 2409, 2502, 5695
HindIII AAGCTT 3 cut(s) 498, 4597, 4763
HpaI GTTAAC 1 cut(s) 2502
HpaII CCGG 5 cut(s) 101, 668, 3422, 4133, 4617
Hpy99I CGWCG 1 cut(s) 5699
HpyCH4IV ACGT 5 cut(s) 915, 1108, 1240, 2686, 5682
HpySE526I ACGT 5 cut(s) 915, 1108, 1240, 2686, 5682
HspAI GCGC 3 cut(s) 59, 2929, 5480
Kpn2I TCCGGA 1 cut(s) 100
KpnI GGTACC 1 cut(s) 3390
Ksp22I TGATCA 1 cut(s) 5853
KspAI GTTAAC 1 cut(s) 2502
LguI GCTCTTC 1 cut(s) 150
LmnI GCTCC 8 cut(s) 103, 899, 1341, 1974, 2082, 3270, 3424, 5011
MabI ACCWGGT 1 cut(s) 1587
MaeII ACGT 5 cut(s) 915, 1108, 1240, 2686, 5682
MbiI CCGCTC 1 cut(s) 5831
MfeI CAATTG 7 cut(s) 62, 1515, 2355, 3366, 3843, 4929, 5860
MflI RGATCY 5 cut(s) 727, 2303, 2475, 2920, 4733
MlsI TGGCCA 1 cut(s) 3826
MluNI TGGCCA 1 cut(s) 3826
MlyI GAGTC 3 cut(s) 77, 3488, 5097
MmeI TCCRAC 5 cut(s) 877, 1475, 1928, 3211, 5459
Mox20I TGGCCA 1 cut(s) 3826
Mph1103I ATGCAT 3 cut(s) 754, 981, 2959
MroI TCCGGA 1 cut(s) 100
MroXI GAANNNNTTC 1 cut(s) 464
MscI TGGCCA 1 cut(s) 3826
MslI CAYNNNNRTG 9 cut(s) 112, 455, 2245, 2385, 2805, 2807, 2939, 3020, 3618
Msp20I TGGCCA 1 cut(s) 3826
MspA1I CMGCKG 4 cut(s) 548, 5392, 5576, 5606
MspCI CTTAAG 1 cut(s) 5098
MspI CCGG 5 cut(s) 101, 668, 3422, 4133, 4617
MspR9I CCNGG 9 cut(s) 669, 1450, 1589, 2543, 2667, 3422, 3432, 4104, 5171
MunI CAATTG 7 cut(s) 62, 1515, 2355, 3366, 3843, 4929, 5860
Mva1269I GAATGC 6 cut(s) 1312, 2196, 2376, 5050, 5099, 5482
MvaI CCWGG 7 cut(s) 1450, 1589, 2543, 2667, 3432, 4104, 5171
NciI CCSGG 2 cut(s) 669, 3422
NcoI CCATGG 2 cut(s) 2069, 3145
NdeI CATATG 3 cut(s) 3595, 4193, 4644
NlaIV GGNNCC 9 cut(s) 105, 1146, 1499, 1938, 3142, 3272, 3388, 3437, 3627
NmeAIII GCCGAG 1 cut(s) 211
NmuCI GTSAC 7 cut(s) 13, 1294, 2019, 2315, 3083, 3777, 5365
NsbI TGCGCA 1 cut(s) 2930
NsiI ATGCAT 3 cut(s) 754, 981, 2959
NspI RCATGY 5 cut(s) 979, 1687, 1933, 2078, 2890
NspV TTCGAA 1 cut(s) 1216
OliI CACNNNNGTG 2 cut(s) 2807, 3618
PaeI GCATGC 2 cut(s) 1933, 2078
PceI AGGCCT 1 cut(s) 3293
PciSI GCTCTTC 1 cut(s) 150
PctI GAATGC 6 cut(s) 1312, 2196, 2376, 5050, 5099, 5482
PdmI GAANNNNTTC 1 cut(s) 464
PflFI GACNNNGTC 1 cut(s) 669
PflMI CCANNNNNTGG 1 cut(s) 4147
PfoI TCCNGGA 1 cut(s) 5169
PinAI ACCGGT 1 cut(s) 4132
PleI GAGTC 3 cut(s) 76, 3488, 5097
PpsI GAGTC 3 cut(s) 76, 3488, 5097
Ppu21I YACGTR 2 cut(s) 1109, 1241
PpuMI RGGWCCY 1 cut(s) 827
PshBI ATTAAT 1 cut(s) 1877
PsiI TTATAA 1 cut(s) 2627
Psp124BI GAGCTC 3 cut(s) 904, 2435, 3247
Psp5II RGGWCCY 1 cut(s) 827
Psp6I CCWGG 7 cut(s) 1448, 1587, 2541, 2665, 3430, 4102, 5169
PspEI GGTNACC 1 cut(s) 3083
PspFI CCCAGC 4 cut(s) 1135, 1529, 2760, 5388
PspGI CCWGG 7 cut(s) 1448, 1587, 2541, 2665, 3430, 4102, 5169
PspN4I GGNNCC 9 cut(s) 105, 1146, 1499, 1938, 3142, 3272, 3388, 3437, 3627
PspPI GGNCC 9 cut(s) 665, 718, 827, 2651, 3140, 3460, 3506, 4009, 5842
PspPPI RGGWCCY 1 cut(s) 827
PstI CTGCAG 4 cut(s) 1699, 4722, 5023, 5121
PstNI CAGNNNCTG 6 cut(s) 1894, 2047, 3863, 4147, 4265, 5081
PsuI RGATCY 5 cut(s) 727, 2303, 2475, 2920, 4733
PsyI GACNNNGTC 1 cut(s) 669
PvuII CAGCTG 2 cut(s) 5392, 5576
RseI CAYNNNNRTG 9 cut(s) 112, 455, 2245, 2385, 2805, 2807, 2939, 3020, 3618
SacI GAGCTC 3 cut(s) 904, 2435, 3247
SalI GTCGAC 1 cut(s) 5693
SapI GCTCTTC 1 cut(s) 150
Sau96I GGNCC 9 cut(s) 665, 718, 827, 2651, 3140, 3460, 3506, 4009, 5842
ScaI AGTACT 1 cut(s) 3689
SchI GAGTC 3 cut(s) 77, 3488, 5097
ScrFI CCNGG 9 cut(s) 669, 1450, 1589, 2543, 2667, 3422, 3432, 4104, 5171
SexAI ACCWGGT 1 cut(s) 1587
SfcI CTRYAG 5 cut(s) 1695, 4208, 4718, 5019, 5117
SfuI TTCGAA 1 cut(s) 1216
SinI GGWCC 4 cut(s) 665, 827, 2651, 5842
SmiMI CAYNNNNRTG 9 cut(s) 112, 455, 2245, 2385, 2805, 2807, 2939, 3020, 3618
SmlI CTYRAG 7 cut(s) 2159, 2535, 2741, 2836, 3604, 3740, 5098
SmoI CTYRAG 7 cut(s) 2159, 2535, 2741, 2836, 3604, 3740, 5098
SnaBI TACGTA 1 cut(s) 1109
SphI GCATGC 2 cut(s) 1933, 2078
SseBI AGGCCT 1 cut(s) 3293
SspI AATATT 3 cut(s) 3834, 3871, 4016
SstI GAGCTC 3 cut(s) 904, 2435, 3247
StuI AGGCCT 1 cut(s) 3293
StyD4I CCNGG 9 cut(s) 667, 1448, 1587, 2541, 2665, 3420, 3430, 4102, 5169
StyI CCWWGG 7 cut(s) 162, 325, 815, 2069, 3145, 4024, 4554
TaiI ACGT 5 cut(s) 918, 1111, 1243, 2689, 5685
TaqI TCGA 9 cut(s) 120, 1216, 1899, 2859, 3241, 5226, 5508, 5694, 5815
TaqII GACCGA 2 cut(s) 894, 4706
TatI WGTACW 6 cut(s) 49, 1774, 3260, 3687, 4897, 5630
TauI GCSGC 1 cut(s) 551
TseFI GTSAC 7 cut(s) 13, 1294, 2019, 2315, 3083, 3777, 5365
Tsp45I GTSAC 7 cut(s) 13, 1294, 2019, 2315, 3083, 3777, 5365
TspGWI ACGGA 4 cut(s) 734, 2785, 5345, 5397
Tth111I GACNNNGTC 1 cut(s) 669
Van91I CCANNNNNTGG 1 cut(s) 4147
Vha464I CTTAAG 1 cut(s) 5098
VneI GTGCAC 3 cut(s) 1242, 2798, 4576
VpaK11BI GGWCC 4 cut(s) 665, 827, 2651, 5842
VspI ATTAAT 1 cut(s) 1877
XapI RAATTY 6 cut(s) 482, 1154, 1321, 3097, 3771, 5161
XbaI TCTAGA 2 cut(s) 308, 1072
XceI RCATGY 5 cut(s) 979, 1687, 1933, 2078, 2890
XcmI CCANNNNNNNNNTGG 3 cut(s) 2549, 3281, 5714
XmiI GTMKAC 2 cut(s) 1782, 5694
XmnI GAANNNNTTC 1 cut(s) 464
ZrmI AGTACT 1 cut(s) 3689
Zsp2I ATGCAT 3 cut(s) 754, 981, 2959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.