pycom12g02370

Essential component of the cytosolic iron-sulfur (Fe S) protein assembly machinery. Required for the maturation of extramitochondrial Fe S proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007416
Physical Location & Seq
Reverse (-)
2047103 .. 2050879
3777 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1044 bp
ATGGAGTTAGTAAAAGAGCTGCGAGAGATTCAGAAGCTAGAGGGCCACACTGATAGGGTCTGGAGCCTCGCCTGGAATCCGGCCACCGGTGTCGCCGGAATTCCACTTTCCTTCGCTTCCTGCAGCGGCGATAAGACCGTTCGTATTTGGGAACAAAACCCATCCACTTCTTCTTGGGATTGTAAGGCAGTTCTGGATGAAACGCACACCAGAACAGTCCGATCGTGCGCTTGGTCCCCATCTGGGAAGTTATTGGCGACTGCAAGTTTCGATGCCACCACTGCAATTTGGGAAAATGTTGGAGGCGATTACGAATGTGTTGCCAGTCTAGAGGGTCATGAAAACGAAGTGAAAAGTGTGTGTTGGAATGCATCTGGGTCTATGCTTACGACTTGCGGTCGGGATAAAACTGTATGGGTATGGGAAGCGCTGCCCGGGAATGAGTTCGACTGTGTTTCAGTGTTGCAAGGCCATACACAGGATGTGAAGATGGTCCAGTGGCATCCCACTAGAAATCTTATATTTTCTTGTAGCTATGATAACACTGTCAAGATATGGGCAGATGATGGTGATGATGATGATTGGTCGTGTGTTCAAACATTAGGTGAAGCTAACAATGGTCACTCTTCTACCGTCTGGGCTCTATCGTTTAATGCCAGTGGAGACAAAATGGTTACCTGCAGTGATGATCTTATTTTAAAGATATGGGGATCAGACGATGCAAAGATGTCTGCTGATGGCTTTGCTCCTTGGCGACATCTTTGCACTCTTTCAGGTTATCATGACCGGACAATATTTTCAGTTCATTGGTCAAGGGACAATATCATAGCCACTGGAGCTGCTGATGATGCCATAAAATTTTTTATGGAGAACAATGATCAAGATGGTTTGGTTGATGGACCTTCTTATAAATTGCTTTTGAGGAAGGAGAATGCCCATGACATGGATATCAATTCTGTGCAATGGAGCCCTGGGGAGGACCGGGTTCTTGCTTCTGCATCCGATGACGGGACGATTAAAATATGGGAGCTGACATCTGAATAG

Protein Analysis

348

Amino Acids

38.46

Weight (kDa)

4.62

Isoelectric Point (pI)

28.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 5 - 99 1.3e-13 WDR5 beta-propeller domain
WD40_Gbeta PF25391 8 - 235 1.5e-09 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 8 - 97 8e-08 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 8 - 98 1.5e-10 Prp19 WD40 domain
WD40 PF00400 9 - 50 6.3e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 9 - 54 2.1e-06 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 10 - 238 1.7e-23 WDR3 second beta-propeller domain
Beta-prop_Aladin PF25460 19 - 142 9.6e-06 Aladin seven-bladed propeller
Beta-prop_EML_2 PF23414 20 - 187 1.5e-14 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 36 - 185 5.4e-13 CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st PF24817 56 - 123 2.7e-06 WDHD1 first WD40 domain
WD40 PF00400 61 - 97 1.4e-06 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 66 - 224 3.8e-15 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 67 - 242 1.8e-33 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 67 - 236 5.1e-25 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 68 - 287 7.2e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 69 - 146 6.2e-17 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 103 - 236 4e-08 TEP-1 second beta-propeller
WD40 PF00400 105 - 141 9.6e-10 WD domain, G-beta repeat
EIF3I PF24805 109 - 187 1.1e-07 EIF3I
Beta-prop_THOC3 PF25174 113 - 187 5.6e-17 THOC3 beta-propeller domain
Beta-prop_Aladin PF25460 114 - 197 7.5e-07 Aladin seven-bladed propeller
WD40_Prp19 PF24814 131 - 237 1.5e-15 Prp19 WD40 domain
WD40 PF00400 150 - 186 5.4e-10 WD domain, G-beta repeat
EIF3I PF24805 154 - 345 1.8e-07 EIF3I
WDR55 PF24796 160 - 287 6.3e-06 WDR55
WD40 PF00400 196 - 236 3.5e-06 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 204 - 288 1.4e-06 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 208 - 288 6.9e-08 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 212 - 346 3.7e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 259 - 345 7.2e-09 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 260 - 345 8.1e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 261 - 346 9.1e-10 THOC3 beta-propeller domain
WD40_Prp19 PF24814 266 - 345 2.9e-06 Prp19 WD40 domain
WD40 PF00400 309 - 342 2.8e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 909
Acc36I ACCTGC 1 cut(s) 686
AccB7I CCANNNNNTGG 1 cut(s) 943
AciI CCGC 2 cut(s) 126, 396
AclWI GGATC 1 cut(s) 718
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 2 cut(s) 99, 857
AfeI AGCGCT 1 cut(s) 429
AfiI CCNNNNNNNGG 6 cut(s) 86, 243, 478, 943, 976, 1008
AgeI ACCGGT 1 cut(s) 86
AgsI TTSAA 1 cut(s) 596
AhdI GACNNNNNGTC 1 cut(s) 396
AjnI CCWGG 2 cut(s) 71, 970
AluBI AGCT 6 cut(s) 19, 37, 534, 611, 839, 1030
AluI AGCT 6 cut(s) 19, 37, 534, 611, 839, 1030
Alw26I GTCTC 1 cut(s) 657
AlwI GGATC 1 cut(s) 718
Ama87I CYCGRG 1 cut(s) 434
Aor51HI AGCGCT 1 cut(s) 429
AoxI GGCC 3 cut(s) 43, 81, 469
ApeKI GCWGC 4 cut(s) 19, 123, 430, 839
ApoI RAATTY 2 cut(s) 99, 857
AsiGI ACCGGT 1 cut(s) 86
Asp700I GAANNNNTTC 1 cut(s) 443
AspLEI GCGC 2 cut(s) 230, 430
AspS9I GGNCC 5 cut(s) 43, 234, 493, 899, 979
AsuC2I CCSGG 3 cut(s) 435, 436, 983
AsuHPI GGTGA 2 cut(s) 581, 617
AvaI CYCGRG 1 cut(s) 434
AvaII GGWCC 4 cut(s) 234, 493, 899, 979
BanII GRGCYC 2 cut(s) 643, 971
BbvI GCAGC 4 cut(s) 6, 135, 417, 826
BccI CCATC 7 cut(s) 169, 247, 484, 560, 731, 878, 890
BcgI CGANNNNNNTGC 4 cut(s) 302, 336, 744, 778
BciT130I CCWGG 2 cut(s) 73, 972
BclI TGATCA 1 cut(s) 877
BcnI CCSGG 3 cut(s) 435, 436, 983
BcoDI GTCTC 1 cut(s) 657
BfaI CTAG 3 cut(s) 38, 329, 510
BfmI CTRYAG 2 cut(s) 121, 679
BfoI RGCGCY 1 cut(s) 431
BfuAI ACCTGC 1 cut(s) 686
BisI GCNGC 5 cut(s) 20, 124, 127, 431, 840
BlsI GCNGC 5 cut(s) 21, 125, 128, 432, 841
Bme1390I CCNGG 5 cut(s) 73, 435, 436, 972, 983
Bme18I GGWCC 4 cut(s) 234, 493, 899, 979
BmeRI GACNNNNNGTC 1 cut(s) 396
BmeT110I CYCGRG 1 cut(s) 434
BmgT120I GGNCC 5 cut(s) 43, 234, 493, 899, 979
BmiI GGNNCC 3 cut(s) 65, 236, 968
BmrFI CCNGG 5 cut(s) 73, 435, 436, 972, 983
BmsI GCATC 6 cut(s) 262, 380, 511, 709, 838, 1007
BpmI CTGGAG 2 cut(s) 82, 855
BpuMI CCSGG 3 cut(s) 435, 436, 983
BsaJI CCNNGG 4 cut(s) 434, 749, 970, 971
BsaWI WCCGGW 2 cut(s) 86, 786
Bsc4I CCNNNNNNNGG 6 cut(s) 86, 243, 478, 943, 976, 1008
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 4 cut(s) 324, 496, 657, 838
Bse3DI GCAATG 1 cut(s) 968
BseBI CCWGG 2 cut(s) 73, 972
BseDI CCNNGG 4 cut(s) 434, 749, 970, 971
BseGI GGATG 5 cut(s) 161, 202, 487, 502, 998
BseLI CCNNNNNNNGG 6 cut(s) 86, 243, 478, 943, 976, 1008
BseMI GCAATG 1 cut(s) 968
BseNI ACTGG 4 cut(s) 324, 496, 657, 838
BseXI GCAGC 4 cut(s) 6, 135, 417, 826
Bsh1285I CGRYCG 2 cut(s) 224, 400
BshFI GGCC 3 cut(s) 45, 83, 471
BshTI ACCGGT 1 cut(s) 86
BsiEI CGRYCG 2 cut(s) 224, 400
BsiHKCI CYCGRG 1 cut(s) 434
BsiSI CCGG 6 cut(s) 80, 87, 96, 435, 787, 982
BslFI GGGAC 3 cut(s) 220, 830, 1024
BslI CCNNNNNNNGG 6 cut(s) 86, 243, 478, 943, 976, 1008
BsmAI GTCTC 1 cut(s) 657
BsmFI GGGAC 3 cut(s) 220, 830, 1024
BsmI GAATGC 2 cut(s) 373, 937
BsnI GGCC 3 cut(s) 45, 83, 471
BsoBI CYCGRG 1 cut(s) 434
Bsp1286I GDGCHC 2 cut(s) 643, 971
Bsp143I GATC 4 cut(s) 221, 688, 710, 877
BspACI CCGC 2 cut(s) 126, 396
BspANI GGCC 3 cut(s) 45, 83, 471
BspHI TCATGA 2 cut(s) 337, 781
BspLI GGNNCC 3 cut(s) 65, 236, 968
BspMAI CTGCAG 2 cut(s) 125, 683
BspMI ACCTGC 1 cut(s) 686
BspPI GGATC 1 cut(s) 718
BsrDI GCAATG 1 cut(s) 968
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 4 cut(s) 324, 496, 657, 838
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 4 cut(s) 434, 749, 970, 971
BssMI GATC 4 cut(s) 221, 688, 710, 877
BssT1I CCWWGG 1 cut(s) 749
Bst2UI CCWGG 2 cut(s) 73, 972
Bst4CI ACNGT 6 cut(s) 139, 217, 412, 452, 547, 634
Bst6I CTCTTC 1 cut(s) 631
BstEII GGTNACC 1 cut(s) 673
BstF5I GGATG 5 cut(s) 161, 202, 487, 502, 998
BstH2I RGCGCY 1 cut(s) 431
BstHHI GCGC 2 cut(s) 230, 430
BstKTI GATC 4 cut(s) 224, 691, 713, 880
BstMAI GTCTC 1 cut(s) 657
BstMBI GATC 4 cut(s) 221, 688, 710, 877
BstMCI CGRYCG 2 cut(s) 224, 400
BstMWI GCNNNNNNNGC 3 cut(s) 281, 836, 848
BstNI CCWGG 2 cut(s) 73, 972
BstPI GGTNACC 1 cut(s) 673
BstSCI CCNGG 5 cut(s) 71, 433, 434, 970, 981
BstSFI CTRYAG 2 cut(s) 121, 679
BstV1I GCAGC 4 cut(s) 6, 135, 417, 826
BsuRI GGCC 3 cut(s) 45, 83, 471
BtsCI GGATG 5 cut(s) 161, 202, 487, 502, 998
BtsI GCAGTG 2 cut(s) 279, 688
BtsIMutI CAGTG 8 cut(s) 48, 279, 465, 503, 543, 664, 688, 831
BveI ACCTGC 1 cut(s) 686
CciI TCATGA 2 cut(s) 337, 781
CfoI GCGC 2 cut(s) 230, 430
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 5 cut(s) 43, 234, 493, 899, 979
Cfr9I CCCGGG 1 cut(s) 434
CspAI ACCGGT 1 cut(s) 86
CviAII CATG 4 cut(s) 338, 782, 938, 943
DpnI GATC 4 cut(s) 223, 690, 712, 879
DpnII GATC 4 cut(s) 221, 688, 710, 877
DraI TTTAAA 1 cut(s) 699
DriI GACNNNNNGTC 1 cut(s) 396
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 1 cut(s) 631
Eam1105I GACNNNNNGTC 1 cut(s) 396
EarI CTCTTC 1 cut(s) 631
Eco130I CCWWGG 1 cut(s) 749
Eco24I GRGCYC 2 cut(s) 643, 971
Eco32I GATATC 1 cut(s) 949
Eco47I GGWCC 4 cut(s) 234, 493, 899, 979
Eco47III AGCGCT 1 cut(s) 429
Eco88I CYCGRG 1 cut(s) 434
Eco91I GGTNACC 1 cut(s) 673
EcoO65I GGTNACC 1 cut(s) 673
EcoRI GAATTC 1 cut(s) 99
EcoRII CCWGG 2 cut(s) 71, 970
EcoRV GATATC 1 cut(s) 949
EcoT14I CCWWGG 1 cut(s) 749
EcoT22I ATGCAT 1 cut(s) 373
EcoT38I GRGCYC 2 cut(s) 643, 971
ErhI CCWWGG 1 cut(s) 749
FaeI CATG 4 cut(s) 341, 785, 941, 946
FaqI GGGAC 3 cut(s) 220, 830, 1024
FatI CATG 4 cut(s) 337, 781, 937, 942
FbaI TGATCA 1 cut(s) 877
Fnu4HI GCNGC 5 cut(s) 20, 124, 127, 431, 840
FokI GGATG 5 cut(s) 148, 209, 489, 494, 985
FriOI GRGCYC 2 cut(s) 643, 971
Fsp4HI GCNGC 5 cut(s) 20, 124, 127, 431, 840
FspBI CTAG 3 cut(s) 38, 329, 510
GlaI GCGC 2 cut(s) 229, 429
GluI GCNGC 5 cut(s) 20, 124, 127, 431, 840
GsuI CTGGAG 2 cut(s) 82, 855
HaeII RGCGCY 1 cut(s) 431
HaeIII GGCC 3 cut(s) 45, 83, 471
HapII CCGG 6 cut(s) 80, 87, 96, 435, 787, 982
HhaI GCGC 2 cut(s) 230, 430
Hin1II CATG 4 cut(s) 341, 785, 941, 946
Hin6I GCGC 2 cut(s) 228, 428
HinP1I GCGC 2 cut(s) 228, 428
HinfI GANTC 2 cut(s) 28, 76
HpaII CCGG 6 cut(s) 80, 87, 96, 435, 787, 982
HphI GGTGA 2 cut(s) 581, 617
Hpy188I TCNGA 5 cut(s) 33, 221, 715, 1003, 1039
Hpy188III TCNNGA 8 cut(s) 61, 194, 329, 338, 401, 550, 782, 881
HpyAV CCTTC 3 cut(s) 121, 912, 919
HpyCH4III ACNGT 6 cut(s) 139, 217, 412, 452, 547, 634
HpyF10VI GCNNNNNNNGC 3 cut(s) 281, 836, 848
Hsp92II CATG 4 cut(s) 341, 785, 941, 946
HspAI GCGC 2 cut(s) 228, 428
Ksp22I TGATCA 1 cut(s) 877
Kzo9I GATC 4 cut(s) 221, 688, 710, 877
LmnI GCTCC 5 cut(s) 63, 751, 836, 966, 1027
Lsp1109I GCAGC 4 cut(s) 6, 135, 417, 826
LweI GCATC 6 cut(s) 262, 380, 511, 709, 838, 1007
MaeI CTAG 3 cut(s) 38, 329, 510
MaeIII GTNAC 2 cut(s) 620, 673
MalI GATC 4 cut(s) 223, 690, 712, 879
MboI GATC 4 cut(s) 221, 688, 710, 877
MboII GAAGA 3 cut(s) 162, 499, 618
MhlI GDGCHC 2 cut(s) 643, 971
MluCI AATT 5 cut(s) 99, 285, 857, 911, 952
MmeI TCCRAC 2 cut(s) 280, 344
MnlI CCTC 6 cut(s) 34, 77, 296, 325, 915, 970
Mph1103I ATGCAT 1 cut(s) 373
MroXI GAANNNNTTC 1 cut(s) 443
MseI TTAA 3 cut(s) 651, 698, 1017
MspA1I CMGCKG 1 cut(s) 126
MspI CCGG 6 cut(s) 80, 87, 96, 435, 787, 982
MspR9I CCNGG 5 cut(s) 73, 435, 436, 972, 983
Mva1269I GAATGC 2 cut(s) 373, 937
MvaI CCWGG 2 cut(s) 73, 972
MwoI GCNNNNNNNGC 3 cut(s) 281, 836, 848
NciI CCSGG 3 cut(s) 435, 436, 983
NdeII GATC 4 cut(s) 221, 688, 710, 877
NlaIII CATG 4 cut(s) 341, 785, 941, 946
NlaIV GGNNCC 3 cut(s) 65, 236, 968
NmuCI GTSAC 1 cut(s) 620
NsiI ATGCAT 1 cut(s) 373
PagI TCATGA 2 cut(s) 337, 781
PasI CCCWGGG 1 cut(s) 971
PctI GAATGC 2 cut(s) 373, 937
PdmI GAANNNNTTC 1 cut(s) 443
PfeI GAWTC 2 cut(s) 28, 76
PflMI CCANNNNNTGG 1 cut(s) 943
PinAI ACCGGT 1 cut(s) 86
PkrI GCNGC 5 cut(s) 21, 125, 128, 432, 841
Ple19I CGATCG 1 cut(s) 224
PsiI TTATAA 1 cut(s) 909
Psp6I CCWGG 2 cut(s) 71, 970
PspEI GGTNACC 1 cut(s) 673
PspGI CCWGG 2 cut(s) 71, 970
PspN4I GGNNCC 3 cut(s) 65, 236, 968
PspPI GGNCC 5 cut(s) 43, 234, 493, 899, 979
PstI CTGCAG 2 cut(s) 125, 683
PvuI CGATCG 1 cut(s) 224
SaqAI TTAA 3 cut(s) 651, 698, 1017
SatI GCNGC 5 cut(s) 20, 124, 127, 431, 840
Sau3AI GATC 4 cut(s) 221, 688, 710, 877
Sau96I GGNCC 5 cut(s) 43, 234, 493, 899, 979
ScrFI CCNGG 5 cut(s) 73, 435, 436, 972, 983
SduI GDGCHC 2 cut(s) 643, 971
SfaNI GCATC 6 cut(s) 262, 380, 511, 709, 838, 1007
SfcI CTRYAG 2 cut(s) 121, 679
SgrAI CRCCGGYG 1 cut(s) 86
SinI GGWCC 4 cut(s) 234, 493, 899, 979
SmaI CCCGGG 1 cut(s) 436
Sse9I AATT 5 cut(s) 99, 285, 857, 911, 952
SsiI CCGC 2 cut(s) 126, 396
SspI AATATT 1 cut(s) 795
SspMI CTAG 3 cut(s) 38, 329, 510
StyD4I CCNGG 5 cut(s) 71, 433, 434, 970, 981
StyI CCWWGG 1 cut(s) 749
TaaI ACNGT 6 cut(s) 139, 217, 412, 452, 547, 634
TaqI TCGA 2 cut(s) 270, 447
TasI AATT 5 cut(s) 99, 285, 857, 911, 952
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 2 cut(s) 28, 76
Tru1I TTAA 3 cut(s) 651, 698, 1017
Tru9I TTAA 3 cut(s) 651, 698, 1017
TscAI CASTG 8 cut(s) 55, 286, 465, 503, 550, 664, 688, 838
TseFI GTSAC 1 cut(s) 620
TseI GCWGC 4 cut(s) 19, 123, 430, 839
Tsp45I GTSAC 1 cut(s) 620
TspDTI ATGAA 3 cut(s) 213, 354, 794
TspMI CCCGGG 1 cut(s) 434
TspRI CASTG 8 cut(s) 55, 286, 465, 503, 550, 664, 688, 838
Van91I CCANNNNNTGG 1 cut(s) 943
VpaK11BI GGWCC 4 cut(s) 234, 493, 899, 979
XapI RAATTY 2 cut(s) 99, 857
XbaI TCTAGA 1 cut(s) 328
XmaI CCCGGG 1 cut(s) 434
XmnI GAANNNNTTC 1 cut(s) 443
XspI CTAG 3 cut(s) 38, 329, 510
Zsp2I ATGCAT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.