pycom12g03170

Transcriptional repressor, ovate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0005069
Physical Location & Seq
Forward (+)
2854238 .. 2856192
1955 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 627 bp
ATGGGGAAGAAAATGAAGCTCCCTTTTCTCTCCAAGAACACATCAGAACTATCAAGATCTTCATCTTCTTCTTCCTCCCCATGGCCTTGGCCTTCTTGTGCTCAAACCAGAACCCTTTCTTTCAGAACAGGCAATGATATCTTCAAGACCATAAACTCAGCCTACTTTGACACCACAAACCCTACAACAGATTTGGTGCTGGTGGATAGCACACCGGACTCGTTCTTCACCAACTCATCATTTGACGAGTGTCCCAGCTTCTCCACCGTCTCTGAAGACTCAAAAGGCGGTGGAGGAGCTGGAGGAGGAGACGCAATAGAGAGTGTGATTAAAGGGTTGAGGTCAGAGAGGCTCTTCTTTGAGCCAGCTGGTCAGACAAGCTCTGTGTTAGTTGAGACCAAGGAGGCTGAGGCGGGGGCCGCGGCAGCAGCAACAGCTGATGAAGGTGGTGATGGGGTTAATAATGTTATGCCATTCAAGGAGAGTGTGGCTTTGTCAATTGACTCACCAGACCCGATTGTGGATTTCAGAAAATCTATGGAGGAAATGGTGGAAGCTCATGGCTTGAAGGATTGGGAGAATCTTGAGGACTTTTGTCGATCTCTTGAGATGATCTTGTGCAGCTGA

Protein Analysis

209

Amino Acids

22.31

Weight (kDa)

4.44

Isoelectric Point (pI)

50.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ovate PF04844 169 - 200 6.4e-09 Transcriptional repressor, ovate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 422
AciI CCGC 4 cut(s) 288, 413, 420, 422
AcuI CTGAAG 1 cut(s) 294
AfiI CCNNNNNNNGG 2 cut(s) 81, 520
AgsI TTSAA 3 cut(s) 145, 478, 568
AluBI AGCT 8 cut(s) 19, 258, 299, 368, 381, 437, 557, 624
AluI AGCT 8 cut(s) 19, 258, 299, 368, 381, 437, 557, 624
Alw21I GWGCWC 1 cut(s) 103
Alw26I GTCTC 3 cut(s) 274, 303, 389
AoxI GGCC 3 cut(s) 83, 89, 417
ApeKI GCWGC 3 cut(s) 425, 428, 621
Asp700I GAANNNNTTC 1 cut(s) 115
AspS9I GGNCC 1 cut(s) 417
AsuHPI GGTGA 3 cut(s) 220, 461, 498
BbsI GAAGAC 1 cut(s) 282
Bbv12I GWGCWC 1 cut(s) 103
BbvCI CCTCAGC 1 cut(s) 408
BbvI GCAGC 2 cut(s) 437, 440
BccI CCATC 1 cut(s) 446
BcoDI GTCTC 3 cut(s) 274, 303, 389
BglII AGATCT 1 cut(s) 56
BisI GCNGC 5 cut(s) 420, 423, 426, 429, 622
BlsI GCNGC 5 cut(s) 421, 424, 427, 430, 623
BmgT120I GGNCC 1 cut(s) 417
BmiI GGNNCC 1 cut(s) 418
BoxI GACNNNNGTC 2 cut(s) 249, 594
BpiI GAAGAC 1 cut(s) 282
BpmI CTGGAG 1 cut(s) 321
Bpu10I CCTNAGC 1 cut(s) 408
BpuEI CTTGAG 2 cut(s) 605, 626
BsaBI GATNNNNATC 1 cut(s) 61
BsaI GGTCTC 1 cut(s) 389
BsaJI CCNNGG 4 cut(s) 80, 86, 399, 420
BsaWI WCCGGW 1 cut(s) 214
Bsc4I CCNNNNNNNGG 2 cut(s) 81, 520
Bse3DI GCAATG 1 cut(s) 139
Bse8I GATNNNNATC 1 cut(s) 61
BseDI CCNNGG 4 cut(s) 80, 86, 399, 420
BseJI GATNNNNATC 1 cut(s) 61
BseLI CCNNNNNNNGG 2 cut(s) 81, 520
BseMI GCAATG 1 cut(s) 139
BseMII CTCAG 2 cut(s) 171, 399
BseRI GAGGAG 3 cut(s) 309, 318, 321
BseXI GCAGC 2 cut(s) 437, 440
BseYI CCCAGC 1 cut(s) 254
Bsh1236I CGCG 1 cut(s) 422
BshFI GGCC 3 cut(s) 85, 91, 419
BsiHKAI GWGCWC 1 cut(s) 103
BsiSI CCGG 1 cut(s) 215
BslFI GGGAC 1 cut(s) 237
BslI CCNNNNNNNGG 2 cut(s) 81, 520
BsmAI GTCTC 3 cut(s) 274, 303, 389
BsmBI CGTCTC 2 cut(s) 274, 303
BsmFI GGGAC 1 cut(s) 237
BsnI GGCC 3 cut(s) 85, 91, 419
Bso31I GGTCTC 1 cut(s) 389
Bsp1286I GDGCHC 1 cut(s) 103
Bsp143I GATC 3 cut(s) 56, 599, 612
Bsp19I CCATGG 1 cut(s) 80
BspACI CCGC 4 cut(s) 288, 413, 420, 422
BspANI GGCC 3 cut(s) 85, 91, 419
BspCNI CTCAG 2 cut(s) 170, 400
BspFNI CGCG 1 cut(s) 422
BspLI GGNNCC 1 cut(s) 418
BspQI GCTCTTC 1 cut(s) 359
BspTNI GGTCTC 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 139
BssECI CCNNGG 4 cut(s) 80, 86, 399, 420
BssMI GATC 3 cut(s) 56, 599, 612
BssT1I CCWWGG 3 cut(s) 80, 86, 399
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 1 cut(s) 359
BstC8I GCNNGC 1 cut(s) 366
BstDEI CTNAG 2 cut(s) 157, 408
BstDSI CCRYGG 2 cut(s) 80, 420
BstFNI CGCG 1 cut(s) 422
BstKTI GATC 3 cut(s) 59, 602, 615
BstMAI GTCTC 3 cut(s) 274, 303, 389
BstMBI GATC 3 cut(s) 56, 599, 612
BstMWI GCNNNNNNNGC 4 cut(s) 419, 425, 428, 434
BstPAI GACNNNNGTC 2 cut(s) 249, 594
BstUI CGCG 1 cut(s) 422
BstV1I GCAGC 2 cut(s) 437, 440
BstV2I GAAGAC 1 cut(s) 282
BstX2I RGATCY 1 cut(s) 56
BstXI CCANNNNNNTGG 1 cut(s) 87
BstYI RGATCY 1 cut(s) 56
BsuRI GGCC 3 cut(s) 85, 91, 419
BtgI CCRYGG 2 cut(s) 80, 420
Cac8I GCNNGC 1 cut(s) 366
Cfr13I GGNCC 1 cut(s) 417
Cfr42I CCGCGG 1 cut(s) 423
CseI GACGC 1 cut(s) 320
CviAII CATG 2 cut(s) 81, 560
DdeI CTNAG 2 cut(s) 157, 408
DpnI GATC 3 cut(s) 58, 601, 614
DpnII GATC 3 cut(s) 56, 599, 612
Eam1104I CTCTTC 1 cut(s) 359
EarI CTCTTC 1 cut(s) 359
Eco130I CCWWGG 3 cut(s) 80, 86, 399
Eco31I GGTCTC 1 cut(s) 389
Eco32I GATATC 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 294
EcoRV GATATC 1 cut(s) 139
EcoT14I CCWWGG 3 cut(s) 80, 86, 399
ErhI CCWWGG 3 cut(s) 80, 86, 399
Esp3I CGTCTC 2 cut(s) 274, 303
FaeI CATG 2 cut(s) 84, 563
FaiI YATR 5 cut(s) 82, 152, 470, 539, 561
FaqI GGGAC 1 cut(s) 237
FatI CATG 2 cut(s) 80, 559
FauI CCCGC 1 cut(s) 406
Fnu4HI GCNGC 5 cut(s) 420, 423, 426, 429, 622
Fsp4HI GCNGC 5 cut(s) 420, 423, 426, 429, 622
GluI GCNGC 5 cut(s) 420, 423, 426, 429, 622
GsaI CCCAGC 1 cut(s) 258
GsuI CTGGAG 1 cut(s) 321
HaeIII GGCC 3 cut(s) 85, 91, 419
HapII CCGG 1 cut(s) 215
HgaI GACGC 1 cut(s) 320
Hin1II CATG 2 cut(s) 84, 563
HinfI GANTC 4 cut(s) 218, 278, 503, 580
HpaII CCGG 1 cut(s) 215
HphI GGTGA 3 cut(s) 220, 461, 498
Hpy188I TCNGA 6 cut(s) 46, 125, 274, 346, 375, 530
Hpy188III TCNNGA 4 cut(s) 54, 145, 584, 605
HpyAV CCTTC 3 cut(s) 102, 437, 562
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4V TGCA 1 cut(s) 621
HpyF10VI GCNNNNNNNGC 4 cut(s) 419, 425, 428, 434
HpyF3I CTNAG 2 cut(s) 157, 408
Hsp92II CATG 2 cut(s) 84, 563
KspI CCGCGG 1 cut(s) 423
Kzo9I GATC 3 cut(s) 56, 599, 612
LguI GCTCTTC 1 cut(s) 359
LmnI GCTCC 2 cut(s) 24, 296
LpnPI CCDG 9 cut(s) 114, 121, 185, 228, 268, 285, 354, 378, 522
Lsp1109I GCAGC 2 cut(s) 437, 440
MalI GATC 3 cut(s) 58, 601, 614
MboI GATC 3 cut(s) 56, 599, 612
MboII GAAGA 9 cut(s) 19, 51, 57, 60, 63, 133, 217, 287, 346
MfeI CAATTG 1 cut(s) 498
MflI RGATCY 1 cut(s) 56
MhlI GDGCHC 1 cut(s) 103
MluCI AATT 1 cut(s) 498
MlyI GAGTC 3 cut(s) 212, 272, 497
MroXI GAANNNNTTC 1 cut(s) 115
MseI TTAA 2 cut(s) 330, 459
MspA1I CMGCKG 4 cut(s) 368, 422, 437, 624
MspI CCGG 1 cut(s) 215
MunI CAATTG 1 cut(s) 498
MvnI CGCG 1 cut(s) 422
MwoI GCNNNNNNNGC 4 cut(s) 419, 425, 428, 434
NcoI CCATGG 1 cut(s) 80
NdeII GATC 3 cut(s) 56, 599, 612
NlaIII CATG 2 cut(s) 84, 563
NlaIV GGNNCC 1 cut(s) 418
PciSI GCTCTTC 1 cut(s) 359
PdmI GAANNNNTTC 1 cut(s) 115
PfeI GAWTC 1 cut(s) 580
PkrI GCNGC 5 cut(s) 421, 424, 427, 430, 623
PleI GAGTC 3 cut(s) 212, 272, 497
PpsI GAGTC 3 cut(s) 212, 272, 497
PshAI GACNNNNGTC 2 cut(s) 249, 594
PspFI CCCAGC 1 cut(s) 254
PspN4I GGNNCC 1 cut(s) 418
PspPI GGNCC 1 cut(s) 417
PsuI RGATCY 1 cut(s) 56
PvuII CAGCTG 3 cut(s) 368, 437, 624
SacII CCGCGG 1 cut(s) 423
SapI GCTCTTC 1 cut(s) 359
SaqAI TTAA 2 cut(s) 330, 459
SatI GCNGC 5 cut(s) 420, 423, 426, 429, 622
Sau3AI GATC 3 cut(s) 56, 599, 612
Sau96I GGNCC 1 cut(s) 417
SchI GAGTC 3 cut(s) 212, 272, 497
SduI GDGCHC 1 cut(s) 103
Sfr303I CCGCGG 1 cut(s) 423
SgrBI CCGCGG 1 cut(s) 423
SmlI CTYRAG 2 cut(s) 584, 605
SmoI CTYRAG 2 cut(s) 584, 605
Sse9I AATT 1 cut(s) 498
SsiI CCGC 4 cut(s) 288, 413, 420, 422
StyI CCWWGG 3 cut(s) 80, 86, 399
TaaI ACNGT 1 cut(s) 268
TaqI TCGA 1 cut(s) 598
TasI AATT 1 cut(s) 498
TauI GCSGC 2 cut(s) 422, 425
TfiI GAWTC 1 cut(s) 580
Tru1I TTAA 2 cut(s) 330, 459
Tru9I TTAA 2 cut(s) 330, 459
TseI GCWGC 3 cut(s) 425, 428, 621
TspDTI ATGAA 3 cut(s) 29, 51, 456
XmnI GAANNNNTTC 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.