Rw3G017970

transcription repressor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0005069
Physical Location & Seq
Forward (+)
19808863 .. 19810349
1487 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G017970.1

Sequence Viewer

Length: 900 bp
ATGAAGCTTCTCCCTTCTTCTCTGTTCAAGAACACAGCACAAGCACCAGAGCCAAAGTCATCTTCTTCCTCTTGGCCCTGGCCTTCTTGCACACAAACCAGAACCATCTCCTTCAGAATTCCCGGCAATGATATTTTCAAGACCATAAACTCGGCCTACTTCGACACCAACACGCCCGAGTCCTTCTTCACTAACTCGTCTGAGGGCTGCCCCAGTTTCTCCACAACAGTTTCTGAAGAAGAGTCCTCGGGCGGAGGAGGAGATCCGATAGAGACTGTGATCAACAAAGGTTTGAGATCAGAGCGGCTCTTCTTCGAGCCCGGTGAGACCAGCTCTATTTTAGACAAGGAATCTATCAAGGATGATGATGATGAAGAAGAAGATGATGGGGTTGATGATGAACTTGCTGTTGGTGATAATAACATTCCATTCAAGGAGAGTGTGGCTTTGTCAATGGACTCGCCGGACCCGTATGTGGATTTCAGAAAATCCATGGAGGAAATGGTGGAAGCTCATGGTTTGAAGGACTGGGAACAACTTGAAGAGCTTTTGTGTTGGTATTTGAGGGTCAATGGAAAATCAAATCATGGATATATTGTTGGAGCTTTCGTTGATCTTTTGGTAGGTCTCGCATTTAGTACTACTATTTCTCCTCCTTCTTGTTCATGTAGTGAGTACTCTCCTTCTACTCCTTTGTCTTTTTACAACAGTACTACTTCTTGTTCATCTTCTTCTACCTCTACTCCTTGCGTGTCCTCCATTGGACCTGAGGAAGGATCGGAAATGAGTACCAGCATCATTCCTTATTTGTCTTCCCTTTTAGAAGCTGAGGAAGAGATTAATGAAGAACAAGATGATGACGATGATGATGATGATGATGATGATGGTGCTTCATCTTAG

Protein Analysis

299

Amino Acids

32.61

Weight (kDa)

4.05

Isoelectric Point (pI)

58.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ovate PF04844 154 - 210 5e-25 Transcriptional repressor, ovate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 304
AciI CCGC 2 cut(s) 252, 304
AclWI GGATC 2 cut(s) 257, 784
AcsI RAATTY 1 cut(s) 117
AcuI CTGAAG 2 cut(s) 97, 255
AfaI GTAC 4 cut(s) 640, 677, 712, 790
AfiI CCNNNNNNNGG 2 cut(s) 475, 773
AgsI TTSAA 5 cut(s) 28, 139, 433, 523, 542
AjnI CCWGG 1 cut(s) 77
AjuI GAANNNNNNNTTGG 4 cut(s) 46, 78, 393, 425
AluBI AGCT 6 cut(s) 7, 333, 512, 547, 605, 827
AluI AGCT 6 cut(s) 7, 333, 512, 547, 605, 827
Alw26I GTCTC 3 cut(s) 266, 320, 632
AlwI GGATC 2 cut(s) 257, 784
AlwNI CAGNNNCTG 1 cut(s) 233
Ama87I CYCGRG 2 cut(s) 176, 247
AoxI GGCC 3 cut(s) 74, 80, 153
ApeKI GCWGC 1 cut(s) 207
ApoI RAATTY 1 cut(s) 117
AseI ATTAAT 1 cut(s) 840
AspS9I GGNCC 3 cut(s) 75, 466, 764
AsuC2I CCSGG 2 cut(s) 123, 321
AsuHPI GGTGA 2 cut(s) 335, 425
AvaI CYCGRG 2 cut(s) 176, 247
AvaII GGWCC 2 cut(s) 466, 764
AxyI CCTNAGG 1 cut(s) 768
BanII GRGCYC 1 cut(s) 321
BbsI GAAGAC 1 cut(s) 804
BbvCI CCTCAGC 1 cut(s) 828
BbvI GCAGC 1 cut(s) 194
BccI CCATC 3 cut(s) 113, 380, 878
BciT130I CCWGG 1 cut(s) 79
BclI TGATCA 1 cut(s) 279
BcnI CCSGG 2 cut(s) 123, 321
BcoDI GTCTC 3 cut(s) 266, 320, 632
BisI GCNGC 2 cut(s) 208, 305
BlsI GCNGC 2 cut(s) 209, 306
BmcAI AGTACT 3 cut(s) 640, 677, 712
Bme1390I CCNGG 3 cut(s) 79, 123, 321
Bme18I GGWCC 2 cut(s) 466, 764
BmeT110I CYCGRG 2 cut(s) 176, 247
BmgT120I GGNCC 3 cut(s) 75, 466, 764
BmiI GGNNCC 1 cut(s) 468
BmrFI CCNGG 3 cut(s) 79, 123, 321
BmrI ACTGGG 2 cut(s) 207, 538
BmsI GCATC 1 cut(s) 804
BmuI ACTGGG 2 cut(s) 207, 538
BpiI GAAGAC 1 cut(s) 804
BplI GAGNNNNNCTC 2 cut(s) 317, 349
Bpu10I CCTNAGC 1 cut(s) 828
BpuMI CCSGG 2 cut(s) 123, 321
BsaI GGTCTC 2 cut(s) 320, 632
BsaJI CCNNGG 3 cut(s) 77, 246, 492
BsaXI ACNNNNNCTCC 6 cut(s) 594, 624, 634, 664, 727, 757
Bsc4I CCNNNNNNNGG 2 cut(s) 475, 773
Bse1I ACTGG 2 cut(s) 213, 533
Bse21I CCTNAGG 1 cut(s) 768
Bse3DI GCAATG 1 cut(s) 133
BseBI CCWGG 1 cut(s) 79
BseDI CCNNGG 3 cut(s) 77, 246, 492
BseGI GGATG 1 cut(s) 367
BseLI CCNNNNNNNGG 2 cut(s) 475, 773
BseMI GCAATG 1 cut(s) 133
BseMII CTCAG 3 cut(s) 192, 759, 819
BseNI ACTGG 2 cut(s) 213, 533
BseRI GAGGAG 3 cut(s) 270, 273, 642
BseXI GCAGC 1 cut(s) 194
BshFI GGCC 3 cut(s) 76, 82, 155
BsiHKCI CYCGRG 2 cut(s) 176, 247
BsiSI CCGG 3 cut(s) 123, 321, 464
BslI CCNNNNNNNGG 2 cut(s) 475, 773
BsmAI GTCTC 3 cut(s) 266, 320, 632
BsnI GGCC 3 cut(s) 76, 82, 155
Bso31I GGTCTC 2 cut(s) 320, 632
BsoBI CYCGRG 2 cut(s) 176, 247
Bsp1286I GDGCHC 1 cut(s) 321
Bsp143I GATC 5 cut(s) 262, 279, 296, 613, 776
Bsp19I CCATGG 1 cut(s) 492
BspACI CCGC 2 cut(s) 252, 304
BspANI GGCC 3 cut(s) 76, 82, 155
BspCNI CTCAG 3 cut(s) 193, 760, 820
BspLI GGNNCC 1 cut(s) 468
BspPI GGATC 2 cut(s) 257, 784
BspQI GCTCTTC 2 cut(s) 314, 537
BspTNI GGTCTC 2 cut(s) 320, 632
BsrBI CCGCTC 1 cut(s) 304
BsrDI GCAATG 1 cut(s) 133
BsrI ACTGG 2 cut(s) 213, 533
BssECI CCNNGG 3 cut(s) 77, 246, 492
BssMI GATC 5 cut(s) 262, 279, 296, 613, 776
BssT1I CCWWGG 1 cut(s) 492
Bst2UI CCWGG 1 cut(s) 79
Bst4CI ACNGT 3 cut(s) 229, 277, 710
Bst6I CTCTTC 4 cut(s) 234, 314, 537, 828
BstDEI CTNAG 4 cut(s) 201, 768, 828, 897
BstDSI CCRYGG 1 cut(s) 492
BstENI CCTNNNNNAGG 1 cut(s) 771
BstF5I GGATG 1 cut(s) 367
BstKTI GATC 5 cut(s) 265, 282, 299, 616, 779
BstMAI GTCTC 3 cut(s) 266, 320, 632
BstMBI GATC 5 cut(s) 262, 279, 296, 613, 776
BstNI CCWGG 1 cut(s) 79
BstSCI CCNGG 3 cut(s) 77, 121, 319
BstV1I GCAGC 1 cut(s) 194
BstV2I GAAGAC 1 cut(s) 804
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
Bsu36I CCTNAGG 1 cut(s) 768
BsuRI GGCC 3 cut(s) 76, 82, 155
BtgI CCRYGG 1 cut(s) 492
BtsCI GGATG 1 cut(s) 367
CaiI CAGNNNCTG 1 cut(s) 233
Cfr13I GGNCC 3 cut(s) 75, 466, 764
Csp6I GTAC 4 cut(s) 639, 676, 711, 789
CviAII CATG 4 cut(s) 493, 515, 587, 666
CviQI GTAC 4 cut(s) 639, 676, 711, 789
DdeI CTNAG 4 cut(s) 201, 768, 828, 897
DpnI GATC 5 cut(s) 264, 281, 298, 615, 778
DpnII GATC 5 cut(s) 262, 279, 296, 613, 776
Eam1104I CTCTTC 4 cut(s) 234, 314, 537, 828
EarI CTCTTC 4 cut(s) 234, 314, 537, 828
EciI GGCGGA 1 cut(s) 267
Eco130I CCWWGG 1 cut(s) 492
Eco24I GRGCYC 1 cut(s) 321
Eco31I GGTCTC 2 cut(s) 320, 632
Eco47I GGWCC 2 cut(s) 466, 764
Eco57I CTGAAG 2 cut(s) 97, 255
Eco81I CCTNAGG 1 cut(s) 768
Eco88I CYCGRG 2 cut(s) 176, 247
EcoNI CCTNNNNNAGG 1 cut(s) 771
EcoRI GAATTC 1 cut(s) 117
EcoRII CCWGG 1 cut(s) 77
EcoT14I CCWWGG 1 cut(s) 492
EcoT38I GRGCYC 1 cut(s) 321
ErhI CCWWGG 1 cut(s) 492
FaeI CATG 4 cut(s) 496, 518, 590, 669
FaiI YATR 7 cut(s) 146, 474, 494, 516, 588, 594, 667
FatI CATG 4 cut(s) 492, 514, 586, 665
FbaI TGATCA 1 cut(s) 279
Fnu4HI GCNGC 2 cut(s) 208, 305
FokI GGATG 1 cut(s) 374
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 2 cut(s) 208, 305
GluI GCNGC 2 cut(s) 208, 305
HaeIII GGCC 3 cut(s) 76, 82, 155
HapII CCGG 3 cut(s) 123, 321, 464
Hin1II CATG 4 cut(s) 496, 518, 590, 669
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 4 cut(s) 179, 242, 350, 458
HpaII CCGG 3 cut(s) 123, 321, 464
HphI GGTGA 2 cut(s) 335, 425
Hpy188I TCNGA 7 cut(s) 116, 202, 235, 267, 301, 485, 781
Hpy188III TCNNGA 2 cut(s) 28, 139
HpyAV CCTTC 8 cut(s) 24, 93, 121, 193, 517, 666, 693, 767
HpyCH4III ACNGT 3 cut(s) 229, 277, 710
HpyCH4V TGCA 1 cut(s) 90
HpyF3I CTNAG 4 cut(s) 201, 768, 828, 897
Hsp92II CATG 4 cut(s) 496, 518, 590, 669
Ksp22I TGATCA 1 cut(s) 279
Kzo9I GATC 5 cut(s) 262, 279, 296, 613, 776
LguI GCTCTTC 2 cut(s) 314, 537
LmnI GCTCC 1 cut(s) 602
Lsp1109I GCAGC 1 cut(s) 194
LweI GCATC 1 cut(s) 804
MalI GATC 5 cut(s) 264, 281, 298, 615, 778
MbiI CCGCTC 1 cut(s) 304
MboI GATC 5 cut(s) 262, 279, 296, 613, 776
MflI RGATCY 1 cut(s) 262
MhlI GDGCHC 1 cut(s) 321
MluCI AATT 1 cut(s) 117
MlyI GAGTC 3 cut(s) 188, 251, 452
MmeI TCCRAC 1 cut(s) 580
MseI TTAA 1 cut(s) 840
MspI CCGG 3 cut(s) 123, 321, 464
MspR9I CCNGG 3 cut(s) 79, 123, 321
MvaI CCWGG 1 cut(s) 79
NciI CCSGG 2 cut(s) 123, 321
NcoI CCATGG 1 cut(s) 492
NdeII GATC 5 cut(s) 262, 279, 296, 613, 776
NlaIII CATG 4 cut(s) 496, 518, 590, 669
NlaIV GGNNCC 1 cut(s) 468
NmeAIII GCCGAG 1 cut(s) 131
PciSI GCTCTTC 2 cut(s) 314, 537
PcsI WCGNNNNNNNCGW 1 cut(s) 467
PfeI GAWTC 1 cut(s) 350
PkrI GCNGC 2 cut(s) 209, 306
PleI GAGTC 3 cut(s) 187, 250, 452
PpsI GAGTC 3 cut(s) 187, 250, 452
PshBI ATTAAT 1 cut(s) 840
Psp6I CCWGG 1 cut(s) 77
PspGI CCWGG 1 cut(s) 77
PspN4I GGNNCC 1 cut(s) 468
PspPI GGNCC 3 cut(s) 75, 466, 764
PstNI CAGNNNCTG 1 cut(s) 233
PsuI RGATCY 1 cut(s) 262
RsaI GTAC 4 cut(s) 640, 677, 712, 790
RsaNI GTAC 4 cut(s) 639, 676, 711, 789
SapI GCTCTTC 2 cut(s) 314, 537
SaqAI TTAA 1 cut(s) 840
SatI GCNGC 2 cut(s) 208, 305
Sau3AI GATC 5 cut(s) 262, 279, 296, 613, 776
Sau96I GGNCC 3 cut(s) 75, 466, 764
ScaI AGTACT 3 cut(s) 640, 677, 712
SchI GAGTC 3 cut(s) 188, 251, 452
ScrFI CCNGG 3 cut(s) 79, 123, 321
SduI GDGCHC 1 cut(s) 321
SfaNI GCATC 1 cut(s) 804
SinI GGWCC 2 cut(s) 466, 764
Sse9I AATT 1 cut(s) 117
SsiI CCGC 2 cut(s) 252, 304
StyD4I CCNGG 3 cut(s) 77, 121, 319
StyI CCWWGG 1 cut(s) 492
TaaI ACNGT 3 cut(s) 229, 277, 710
TaqI TCGA 2 cut(s) 162, 315
TasI AATT 1 cut(s) 117
TatI WGTACW 3 cut(s) 638, 675, 710
TauI GCSGC 1 cut(s) 307
TfiI GAWTC 1 cut(s) 350
Tru1I TTAA 1 cut(s) 840
Tru9I TTAA 1 cut(s) 840
TseI GCWGC 1 cut(s) 207
TspDTI ATGAA 7 cut(s) 17, 387, 414, 654, 714, 858, 882
VpaK11BI GGWCC 2 cut(s) 466, 764
VspI ATTAAT 1 cut(s) 840
XagI CCTNNNNNAGG 1 cut(s) 771
XapI RAATTY 1 cut(s) 117
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
ZrmI AGTACT 3 cut(s) 640, 677, 712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.