Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0003661

Physical Location & Seq

Genomic Coordinates Reverse (-)

5784690 .. 5803531

Gene Structure 18842 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5055 bp

ATGGCTTCCTCAGAGGTTCCGACGGCGTCCACTGAGGCGATTAAGTTTGCTTTCTTAACTGACGAGGAGGTGCGACAGCACAGCTTTCTGAGGGTTACCGACCCATCTCTCCTTACTCAATTAGGAAACCCCGTGGCCGGTGGGCTATACGATAAGGCACTCGGCCCAATTGATGACAAACCCGGCGAAAGTTGCGGAACATGTGGTGAGAAGTCGCGTAATTGCCCGGGTCATTGTGGTCATATTGACCTTGCGTTGCCAGTTTATAATCCCTTGCTGTTCAATACTCTTTTCAAACTTCTACAAAGAACGTGTTTCTTCTGCTACCATTTTCGGACAGACCAAACACATGTTGAGAAAGCTGTTTCCCAATTAAAACTCATAATGGAAGGTGACGTCGTTGGGGCTAAAATGTTAGATTCTGACTCACCTAGTGAATACTTAGACTGCAACGAGGGAGATAGTCACAGTGACAAGCATGCAAAGCAGCAAGGATGGACTTCTCTTCAGTTTACGGAGCTAATGTCTGTTTTTGATAAGGCTTTGCCGAAACAAGGGAAGGACTGCAACAATTGCAAGGCGAAGAATCCCAAAATCATCAAACCTACTTTTGGATGGTTTCAAATGAGTGGTGCTGAAAATGCGAGAGAAAATGTTATCAGAGGGTGCAACCTGGTGGGACCCTTAACTGGTCAAGATGAAGGGGATGAGAATGAAAATGTTGACAATAATGATATGGAAAAGCACAATGGAGAAGGAAGCAAAGTGCCAAGTGAGTTCCTTAATCAGACAACTTCCTTGTCGGGAGATCTATTACCAAAACAGGTTCAAGACATCATAAAGCTTTTGTGGGACAATGAAGCTGAGTTCTGCTCTTTTATTGGTGATATCCAATGCCAAGGAATTGGAAGAAAAGCAGGTCACTCTATGTTTTTCTTAGAGACTGTACTTGTGCCACCAATCAAATTTCGTCCTCCATCCAAGGGTGGTGATTCTGTGATGGAGCATCCTCAAACCGTTTTATTAAGTAAGGTGGTGCAGGCAAATAATTCTTTGCAAGAGTCTGTTGTTACCAAGTCCGACCCCTCAAAGACAATTCGGCAGTGGATCGATCTTCAAGAGTACATAAATGTGATGTACAATAGCAAAACTGCTACTGGTAAAGGTCAAAAAGATGCGGATCCTGGAATATGTCAGTTGCTTGAGAAGAAGGAAGGCCTGTTCCGGCAGAAAATGATGGGCAAACGGGTTAATTATGCGTGTCGGTCTGTCATATCCCCAGATCCCTATTTAGCTGTTAATGAGATTGGAATTCCTCCGTATTTTGCAACTAGATTAACATATCCTGAGAGAGTTACTCCGTGGAATGTTGACAAGTTGAGGAATGCAATTGTCAACGGTCCTGAAATCCATCCTGGAGCTACACAGTATGTTGATAAGTCCTCTGTGAAGATGTTGAGACAAAACCGGAGAGAACGCATTTCAACAGCAAGAAAATTACCTTCTTCAAAAGGGCTTGCTACACAACACGGAAAAGGATCTGATAATAGCTATGAAAACAAAGTTGTTTTTCGTCACTTGCGAGATGGGGACATTGTGCTTGTCAATCGACAGCCTACACTTCACAAACCCAGTATAATGGCGCATGTAGTTCGTGTATTGAAGGGGGAGAAAACAATTCGTATGCACTATGCAAATTGCAGTACGTACAATGCTGATTTTGATGGTGATGAAATGAATGTTCATTTTCCACAAGATGAAATTTCTCGAGCAGAAGCTTACAATATCGTAAATGCAAATAACCAATATGTAAAGCCTACCAGCGGTGATCCAATTAGAGCCTTGATTCAGGACCATGTCATAAGTGCTGTCCTCCTTACAAAGAAGGATACCTTTTTGCGCTGGGAGGAATTTAATCAGCTTCTTTATAGTTCATGTGTTTCAGCCCGTCGGTCAGATGCTTTGTCTGGAAAACCAGATCAAAAGGTTTTCATGTGTAACATTGAAGGCACAATGCAGCCCCTTCTTCCTGCTATTTGGAAACCAGAGCGTCTTTGGACTGGAAAACAGGTCATCACTGCCTTGCTAAATCATATTACTAGGGGTTCTCCGCCAGTTACTGTAGAAAAGGATGCAAAAATCCCACGTGGTTTTTTTAACTGTAATCTTGAAGAAGAGAAATTTGATGAAGAGGAACTACAGAAAAAGAAGAAACGAAGCAAAGGAAAACAAGCTGATGAATGTGGATCACAGAATGAGAAGAAACCAGACAAAAAAGTAGATAACGATAAATCAAAGGACACAGGGGTTGTAGATGAGAACAGTTTATTAATCTATAAAAACAATTTGGTGCGCGGTGTGATTGACAAGGCTCAGTTTGGTGATTATGGTTTGGTCCATACAGTGCAGGAGCTCTATGGCTCAGATGCTGCAGGAATATTGCTTTCTGTATTGAGTCGTTTGTTTACTGTTTATTTGCAGATGCATGGGTTCACATGTGGTGTTGATGATCTTCTGGTAATGCCGAGCAAAGACACAAAAATGAAGGAACAACTTGAAAGTTGTGAAGAGATTGGTGAAAAAGTTTTCCGTGATTTTATCGAAGTCGAAGATGATAAAAGAAAAGATCTAGTGGCACTGCAATTGAACATTGAAAAATTTATACGGAGTAATGGAGAATCTGCCTTGGCAGCCTTGGATAGGAGAATGATTAGTCAGCTTAATAACAAGACCTCCAACTCTGACGTCTTCAAACAATTATTGTTGAAAGGGTTATCAAAACCGTCCGTTAAGAATTGTATCTATCTTATGACCACATCTGGGGCTAAAGGTAGTGTGGCCAACCTCCAACAAATAACTTCGTATTTGGGTCAGCAAGAGCTAGAAGGCAAACGGGTGCCTCAAATGGTTTCTGGAAAGACCTTACCGTGCTTTCACCCATGGGATTGGTCATCTCGATCTGGAGGCTTTATTATTGATCGTTTTCTTACTGGTCTTCGGCCTCAGGAGTATTATTTTCATTGCATGGCTGGTCGTGAAGGGTTGGTTGATACGGCTGTCAAAACATCTCGTAGTGGGTACCTGCAAAGGTGCCTAATAAAAAATCTTGAGTGCCTAAAAGTTTGTTATGACCATACCGTCCGTGATGCTGACGGTTCCATTGTTCAATTTTATTATGGAGAAGATGGTGTTGATGTCCATTTGACAAGCTTCCTACAAAAATTTGACTCCCTGGCAGCTAATAAAGAAATGTTCCAGAACAAATTCCATCGACAAATCGACAAATCCGATCCTTACATTGCTGAGCTGCCTTCAGCTCTCAAGTATAAAGCAGAGAAGTTCGTGCTTGATTTGTCCTTGAAGGAGGAAAGTCTTAACTTATTCAGCCAAGTGGATTTCTTGAAGTTAATGGAGCATAAATACCTCTCTAGTCTTGCTCAACCTGGAGAACCAGTTGGTGTTCTTGCTGCTCAGTCAGTAGGAGAACCGTCAACTCAGATGACACTGAATACTTTCCATCTTGCTGGAAGAGGTGAAATGAATGTGACACTTGGAATCCCTCGTCTTCAAGAAATCTTGATGACTGCTGCAAATGAGATTAAGACTCCGGTCATGACATGTCCATTGCGAAAAGGGAGATCAAAGGAGGAAGCTCAGCATCTAGCTAATAAGTTGAAGAAGATCACTGTGGCAGACATAATAGAGAGTATGGAAGTTACGTTGTTGCCATTTGTCCTTCAGGGTCTTGAAACTTGCCGTATTTATAAACTTCAAATGAAACTCCACATACCTGAGCATCTTCAAAACATCGTTCAAGATATATCAGAAGATTGGGAGGAAATCCTAGAGGTTATGTTTGTTAGGGCGTTGGAAGATGCCATACAAAGTCATGTGCTCTTGCTTTCCAAAATTAGTGGTATAAAAAATGTCACTGATGCACCACAAAAGGCATCAAATGAGACGGATGAAAATATTTCTAAAAGTGGACATCAACATCAGGAAGAAGACGATGACAATGATGGTGCAGACGATGGTGAAGGGGCTGAGGATTTTGGTATGGATGCACAAAAGCGAAAGCTTCAAGCTTTTGATGAGATGGATTATGAAGATGGATGTGAAGATGAAGTGAGAGATGGAGATCTGACAGATGGAGATGAAGTCGAAAATGATGTGGATAATGATGAGGAGAATGATGTTGAAACTGGTAAGGATGGTGCAATTGAGGTTGTAGATGCTACTGATGAAATGCCTGCAAGTCCTTTAGAAGAGGCTGGAGACCTGCCTAAACCCAAGTCAAAGGAGAAAAAGAACAAATCAGGAGCCACAATTAAAAAGAAAAAGATCAGAGCTCAGTTAGTAAGAAAAGACACCGACAGGTCAATATTTGTGTCAGCGAAGGGGTTCCACTTTGAGGTCCATTTTAAATTTACCGATGAACCTCATATTTTGCTGGCTCAGATTGCTCAGCAGACAGCCCAGAAGGTTTCTATGCAAAGATCTGGCAGTGTTGATGACTGTAAACAAATAACTTGTGACGAAAATCAAGTCCTCTTATACGGAAAGGATCCTGAGAATAAACAAAGTTATTCATCCAAGGAAAAAAAGGAGATGGCAGCTCTGCAGACTACTGGAGTGGATTTCCGTACATTTTGGAAATTGCAAGATGTTCTTGATGTCAGGTATTTATATTCCAACAACATACATGCTATGCTGAACACTTACGGAGTTGAAGCCGCCAGGGAAACTATCATCAGAGAAATTATGAACGTTTTTAAGTCTTACGGGATATCAGTCAACATTAGGCACCTTACGCTTATAGCTGACTACATGACTCACTCGGGTGGTTATCGACCAATGAATAGATTTGGAGGCATAGCAGAGTCGATATCTCCGTTTAATAAAATGTCTTTTGAGACAGCTTCTAAGTTCATTGTCGAAGCAGCATATCACGGGCAGACGGATGACTTGGAAACGCCATCCTCGAGGATCTGTCTTGGATTGCCAGTCAAGGTAGGCACTGGATGTTTTGATTTGATGCAGAAGATAGAGGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005736 GO:0005911 GO:0006139 GO:0006351 GO:0006360 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009987 GO:0010467 GO:0016070 GO:0018130 GO:0019438 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055029 GO:0055044 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1685

Amino Acids

188.95

Weight (kDa)

5.75

Isoelectric Point (pI)

42.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	12 - 411	1.1e-28	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	414 - 598	4.6e-60	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	602 - 838	9.1e-29	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	899 - 991	1.9e-20	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	998 - 1623	5.4e-85	RNA polymerase Rpb1, domain 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57660 AT3G57660
fragaria_vesca	FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g35030 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210
malus_domestica	MD12G1066600.v1.1 MD14G1066700.v1.1
prunus_persica	Prupe.7G067700_v2.0.a1 Prupe.7G067700_v2.0.a1 Prupe.7G067700_v2.0.a1
pyrus_communis	pycom12g05800 pycom14g05430
rosa_chinensis	RchiOBHm_Chr3g0481021
rosa_laevigata	RLG00000023431
rosa_multiflora	Rmu_sc0002927.1_g000058
rosa_roxburghii	Rroxscaffold_6G00400730
rosa_rugosa	Rorug03G0189100
rosa_samantha	Rh3AG240000 Rh3BG275100 Rh3CG270600 Rh3DG266500
rosa_wichuraiana	Rw0G008430 Rw3G021430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	267, 3765
AasI	GACNNNNNNGTC	1 cut(s)	3137
AatII	GACGTC	2 cut(s)	399, 2748
AbsI	CCTCGAGG	1 cut(s)	4981
Acc36I	ACCTGC	3 cut(s)	908, 3090, 4287
Acc65I	GGTACC	1 cut(s)	3078
AccB1I	GGYRCC	4 cut(s)	2896, 3078, 3090, 4803
AccII	CGCG	2 cut(s)	217, 2355
AciI	CCGC	6 cut(s)	195, 1178, 1824, 2111, 2355, 4734
AclI	AACGTT	1 cut(s)	4767
AcoI	YGGCCR	2 cut(s)	135, 2838
AcsI	RAATTY	9 cut(s)	965, 1311, 1761, 1910, 2180, 2657, 3221, 3263, 4424
AcuI	CTGAAG	3 cut(s)	491, 3297, 3722
AcvI	CACGTG	1 cut(s)	2147
AcyI	GRCGYC	3 cut(s)	26, 396, 2745
AdeI	CACNNNGTG	1 cut(s)	434
AfaI	GTAC	7 cut(s)	948, 1124, 1139, 1705, 1709, 3080, 4645
AfiI	CCNNNNNNNGG	9 cut(s)	137, 554, 611, 689, 983, 1823, 2700, 2820, 4411
AflIII	ACRYGT	5 cut(s)	200, 311, 349, 2495, 3617
AjnI	CCWGG	6 cut(s)	672, 1183, 1414, 3231, 3442, 4736
AleI	CACNNNNGTG	1 cut(s)	4839
AloI	GAACNNNNNNTCC	2 cut(s)	1205, 1237
Alw21I	GWGCWC	3 cut(s)	2415, 3897, 4351
Alw26I	GTCTC	5 cut(s)	935, 1453, 3953, 4269, 4907
AlwNI	CAGNNNCTG	2 cut(s)	2120, 2429
Ama87I	CYCGRG	4 cut(s)	226, 1767, 4837, 4981
AoxI	GGCC	5 cut(s)	135, 163, 1216, 2838, 2999
ApoI	RAATTY	9 cut(s)	965, 1311, 1761, 1910, 2180, 2657, 3221, 3263, 4424
ArsI	GACNNNNNNTTYG	2 cut(s)	4530, 4562
AseI	ATTAAT	1 cut(s)	2330
Asp700I	GAANNNNTTC	3 cut(s)	3263, 3512, 4400
Asp718I	GGTACC	1 cut(s)	3078
AspLEI	GCGC	3 cut(s)	1645, 1902, 2355
AspS9I	GGNCC	6 cut(s)	164, 680, 1400, 1852, 2395, 4414
AsuC2I	CCSGG	3 cut(s)	183, 227, 228
AvaI	CYCGRG	4 cut(s)	226, 1767, 4837, 4981
AvaII	GGWCC	5 cut(s)	680, 1400, 1852, 2395, 4414
AxyI	CCTNAGG	1 cut(s)	3003
BaeI	ACNNNNGTAYC	2 cut(s)	3042, 3075
BalI	TGGCCA	1 cut(s)	2840
BamHI	GGATCC	2 cut(s)	1180, 4564
BanI	GGYRCC	4 cut(s)	2896, 3078, 3090, 4803
BanII	GRGCYC	2 cut(s)	2415, 4351
BarI	GAAGNNNNNNTAC	4 cut(s)	2181, 2213, 3864, 3896
BbrPI	CACGTG	1 cut(s)	2147
BbsI	GAAGAC	4 cut(s)	2740, 2987, 3557, 4011
Bbv12I	GWGCWC	3 cut(s)	2415, 3897, 4351
BbvCI	CCTCAGC	1 cut(s)	4044
BceAI	ACGGC	3 cut(s)	39, 3069, 3741
BcgI	CGANNNNNNTGC	2 cut(s)	1634, 1668
BciT130I	CCWGG	6 cut(s)	674, 1185, 1416, 3233, 3444, 4738
BciVI	GTATCC	1 cut(s)	1882
BcnI	CCSGG	3 cut(s)	183, 227, 228
BcoDI	GTCTC	5 cut(s)	935, 1453, 3953, 4269, 4907
BfaI	CTAG	8 cut(s)	432, 1332, 2100, 2630, 2882, 3429, 3662, 3845
BfmI	CTRYAG	4 cut(s)	2121, 2198, 2430, 4619
BfuAI	ACCTGC	3 cut(s)	908, 3090, 4287
BfuI	GTATCC	1 cut(s)	1882
BglII	AGATCT	4 cut(s)	808, 2626, 4138, 4496
BlpI	GCTNAGC	3 cut(s)	3303, 3654, 4464
Bme1390I	CCNGG	9 cut(s)	183, 227, 228, 674, 1185, 1416, 3233, 3444, 4738
Bme18I	GGWCC	5 cut(s)	680, 1400, 1852, 2395, 4414
BmeT110I	CYCGRG	4 cut(s)	226, 1767, 4837, 4981
BmgT120I	GGNCC	6 cut(s)	164, 680, 1400, 1852, 2395, 4414
BmrFI	CCNGG	9 cut(s)	183, 227, 228, 674, 1185, 1416, 3233, 3444, 4738
BmrI	ACTGGG	1 cut(s)	1626
BmuI	ACTGGG	1 cut(s)	1626
BoxI	GACNNNNGTC	1 cut(s)	3608
BpiI	GAAGAC	4 cut(s)	2740, 2987, 3557, 4011
BplI	GAGNNNNNCTC	4 cut(s)	857, 889, 1342, 1374
BpmI	CTGGAG	5 cut(s)	1437, 2982, 3465, 4293, 4650
Bpu10I	CCTNAGC	2 cut(s)	3792, 4044
Bpu1102I	GCTNAGC	3 cut(s)	3303, 3654, 4464
BpuEI	CTTGAG	3 cut(s)	1223, 3128, 3305
BpuMI	CCSGG	3 cut(s)	183, 227, 228
Bsa29I	ATCGAT	1 cut(s)	1110
BsaAI	YACGTR	2 cut(s)	1707, 2147
BsaBI	GATNNNNATC	2 cut(s)	1179, 4137
BsaHI	GRCGYC	3 cut(s)	26, 396, 2745
BsaI	GGTCTC	1 cut(s)	4269
BsaWI	WCCGGW	2 cut(s)	1467, 3607
BsaXI	ACNNNNNCTCC	8 cut(s)	509, 539, 2717, 2747, 3438, 3468, 4143, 4173
Bsc4I	CCNNNNNNNGG	9 cut(s)	137, 554, 611, 689, 983, 1823, 2700, 2820, 4411
Bse118I	RCCGGY	1 cut(s)	137
Bse21I	CCTNAGG	1 cut(s)	3003
Bse3DI	GCAATG	3 cut(s)	3019, 3297, 3623
Bse8I	GATNNNNATC	2 cut(s)	1179, 4137
BseBI	CCWGG	6 cut(s)	674, 1185, 1416, 3233, 3444, 4738
BseCI	ATCGAT	1 cut(s)	1110
BseJI	GATNNNNATC	2 cut(s)	1179, 4137
BseLI	CCNNNNNNNGG	9 cut(s)	137, 554, 611, 689, 983, 1823, 2700, 2820, 4411
BseMI	GCAATG	3 cut(s)	3019, 3297, 3623
BseRI	GAGGAG	2 cut(s)	80, 4199
BseYI	CCCAGC	1 cut(s)	1902
BsgI	GTGCAG	3 cut(s)	1058, 2426, 4044
Bsh1236I	CGCG	2 cut(s)	217, 2355
BshFI	GGCC	5 cut(s)	137, 165, 1218, 2840, 3001
BshNI	GGYRCC	4 cut(s)	2896, 3078, 3090, 4803
BshVI	ATCGAT	1 cut(s)	1110
BsiHKAI	GWGCWC	3 cut(s)	2415, 3897, 4351
BsiHKCI	CYCGRG	4 cut(s)	226, 1767, 4837, 4981
BsiSI	CCGG	6 cut(s)	138, 183, 227, 1225, 1468, 3608
BslFI	GGGAC	3 cut(s)	693, 866, 1604
BslI	CCNNNNNNNGG	9 cut(s)	137, 554, 611, 689, 983, 1823, 2700, 2820, 4411
BsmAI	GTCTC	5 cut(s)	935, 1453, 3953, 4269, 4907
BsmBI	CGTCTC	1 cut(s)	3953
BsmFI	GGGAC	3 cut(s)	693, 866, 1604
BsmI	GAATGC	1 cut(s)	1390
BsnI	GGCC	5 cut(s)	137, 165, 1218, 2840, 3001
Bso31I	GGTCTC	1 cut(s)	4269
BsoBI	CYCGRG	4 cut(s)	226, 1767, 4837, 4981
Bsp1286I	GDGCHC	3 cut(s)	2415, 3897, 4351
Bsp1407I	TGTACA	1 cut(s)	1137
Bsp1720I	GCTNAGC	3 cut(s)	3303, 3654, 4464
Bsp19I	CCATGG	1 cut(s)	2939
BspACI	CCGC	6 cut(s)	195, 1178, 1824, 2111, 2355, 4734
BspANI	GGCC	5 cut(s)	137, 165, 1218, 2840, 3001
BspDI	ATCGAT	1 cut(s)	1110
BspFNI	CGCG	2 cut(s)	217, 2355
BspHI	TCATGA	1 cut(s)	3612
BspMAI	CTGCAG	2 cut(s)	2434, 4623
BspMI	ACCTGC	3 cut(s)	908, 3090, 4287
BspT107I	GGYRCC	4 cut(s)	2896, 3078, 3090, 4803
BspTNI	GGTCTC	1 cut(s)	4269
BsrDI	GCAATG	3 cut(s)	3019, 3297, 3623
BsrFI	RCCGGY	1 cut(s)	137
BsrGI	TGTACA	1 cut(s)	1137
BssAI	RCCGGY	1 cut(s)	137
BssNI	GRCGYC	3 cut(s)	26, 396, 2745
BssT1I	CCWWGG	6 cut(s)	898, 981, 2685, 2694, 2939, 4593
Bst2UI	CCWGG	6 cut(s)	674, 1185, 1416, 3233, 3444, 4738
Bst6I	CTCTTC	6 cut(s)	510, 2169, 2184, 2562, 3523, 4260
BstACI	GRCGYC	3 cut(s)	26, 396, 2745
BstAPI	GCANNNNNTGC	1 cut(s)	573
BstAUI	TGTACA	1 cut(s)	1137
BstBAI	YACGTR	2 cut(s)	1707, 2147
BstC8I	GCNNGC	5 cut(s)	480, 1041, 1518, 4251, 4452
BstDSI	CCRYGG	3 cut(s)	132, 1361, 2939
BstEII	GGTNACC	1 cut(s)	94
BstENI	CCTNNNNNAGG	1 cut(s)	2698
BstFNI	CGCG	2 cut(s)	217, 2355
BstHHI	GCGC	3 cut(s)	1645, 1902, 2355
BstMAI	GTCTC	5 cut(s)	935, 1453, 3953, 4269, 4907
BstMWI	GCNNNNNNNGC	7 cut(s)	192, 484, 573, 641, 2690, 4460, 4810
BstNI	CCWGG	6 cut(s)	674, 1185, 1416, 3233, 3444, 4738
BstNSI	RCATGY	7 cut(s)	204, 353, 482, 1649, 2499, 3621, 4706
BstPAI	GACNNNNGTC	1 cut(s)	3608
BstPI	GGTNACC	1 cut(s)	94
BstSCI	CCNGG	9 cut(s)	181, 225, 226, 672, 1183, 1414, 3231, 3442, 4736
BstSFI	CTRYAG	4 cut(s)	2121, 2198, 2430, 4619
BstSNI	TACGTA	1 cut(s)	1707
BstUI	CGCG	2 cut(s)	217, 2355
BstV2I	GAAGAC	4 cut(s)	2740, 2987, 3557, 4011
BstX2I	RGATCY	9 cut(s)	808, 1180, 1282, 1538, 2626, 4138, 4496, 4564, 4986
BstXI	CCANNNNNNTGG	4 cut(s)	905, 1639, 2946, 3524
BstYI	RGATCY	9 cut(s)	808, 1180, 1282, 1538, 2626, 4138, 4496, 4564, 4986
Bsu15I	ATCGAT	1 cut(s)	1110
Bsu36I	CCTNAGG	1 cut(s)	3003
BsuI	GTATCC	1 cut(s)	1882
BsuRI	GGCC	5 cut(s)	137, 165, 1218, 2840, 3001
BsuTUI	ATCGAT	1 cut(s)	1110
BtgI	CCRYGG	3 cut(s)	132, 1361, 2939
BtsI	GCAGTG	4 cut(s)	1109, 2076, 2636, 4510
BveI	ACCTGC	3 cut(s)	908, 3090, 4287
Cac8I	GCNNGC	5 cut(s)	480, 1041, 1518, 4251, 4452
CaiI	CAGNNNCTG	2 cut(s)	2120, 2429
CciI	TCATGA	1 cut(s)	3612
CfoI	GCGC	3 cut(s)	1645, 1902, 2355
Cfr10I	RCCGGY	1 cut(s)	137
Cfr13I	GGNCC	6 cut(s)	164, 680, 1400, 1852, 2395, 4414
Cfr9I	CCCGGG	1 cut(s)	226
ClaI	ATCGAT	1 cut(s)	1110
CseI	GACGC	2 cut(s)	15, 2039
CsiI	ACCWGGT	1 cut(s)	672
Csp6I	GTAC	7 cut(s)	947, 1123, 1138, 1704, 1708, 3079, 4644
CspCI	CAANNNNNGTGG	2 cut(s)	3895, 3930
CviQI	GTAC	7 cut(s)	947, 1123, 1138, 1704, 1708, 3079, 4644
DraI	TTTAAA	1 cut(s)	4423
DraIII	CACNNNGTG	1 cut(s)	434
DrdI	GACNNNNNNGTC	1 cut(s)	3137
DseDI	GACNNNNNNGTC	1 cut(s)	3137
EaeI	YGGCCR	2 cut(s)	135, 2838
Eam1104I	CTCTTC	6 cut(s)	510, 2169, 2184, 2562, 3523, 4260
EarI	CTCTTC	6 cut(s)	510, 2169, 2184, 2562, 3523, 4260
EciI	GGCGGA	1 cut(s)	2100
Ecl136II	GAGCTC	2 cut(s)	2413, 4349
Eco105I	TACGTA	1 cut(s)	1707
Eco130I	CCWWGG	6 cut(s)	898, 981, 2685, 2694, 2939, 4593
Eco147I	AGGCCT	1 cut(s)	1218
Eco24I	GRGCYC	2 cut(s)	2415, 4351
Eco31I	GGTCTC	1 cut(s)	4269
Eco32I	GATATC	3 cut(s)	889, 4788, 4887
Eco47I	GGWCC	5 cut(s)	680, 1400, 1852, 2395, 4414
Eco53kI	GAGCTC	2 cut(s)	2413, 4349
Eco57I	CTGAAG	3 cut(s)	491, 3297, 3722
Eco72I	CACGTG	1 cut(s)	2147
Eco81I	CCTNAGG	1 cut(s)	3003
Eco88I	CYCGRG	4 cut(s)	226, 1767, 4837, 4981
Eco91I	GGTNACC	1 cut(s)	94
EcoICRI	GAGCTC	2 cut(s)	2413, 4349
EcoNI	CCTNNNNNAGG	1 cut(s)	2698
EcoO109I	RGGNCCY	1 cut(s)	680
EcoO65I	GGTNACC	1 cut(s)	94
EcoRI	GAATTC	1 cut(s)	1311
EcoRII	CCWGG	6 cut(s)	672, 1183, 1414, 3231, 3442, 4736
EcoRV	GATATC	3 cut(s)	889, 4788, 4887
EcoT14I	CCWWGG	6 cut(s)	898, 981, 2685, 2694, 2939, 4593
EcoT22I	ATGCAT	1 cut(s)	2487
EcoT38I	GRGCYC	2 cut(s)	2415, 4351
ErhI	CCWWGG	6 cut(s)	898, 981, 2685, 2694, 2939, 4593
Esp3I	CGTCTC	1 cut(s)	3953
FalI	AAGNNNNNCTT	4 cut(s)	1877, 1909, 4653, 4685
FaqI	GGGAC	3 cut(s)	693, 866, 1604
FriOI	GRGCYC	2 cut(s)	2415, 4351
FspBI	CTAG	8 cut(s)	432, 1332, 2100, 2630, 2882, 3429, 3662, 3845
GlaI	GCGC	3 cut(s)	1644, 1901, 2354
GsaI	CCCAGC	1 cut(s)	1906
GsuI	CTGGAG	5 cut(s)	1437, 2982, 3465, 4293, 4650
HaeIII	GGCC	5 cut(s)	137, 165, 1218, 2840, 3001
HapII	CCGG	6 cut(s)	138, 183, 227, 1225, 1468, 3608
HgaI	GACGC	2 cut(s)	15, 2039
HhaI	GCGC	3 cut(s)	1645, 1902, 2355
Hin1I	GRCGYC	3 cut(s)	26, 396, 2745
Hin6I	GCGC	3 cut(s)	1643, 1900, 2353
HinP1I	GCGC	3 cut(s)	1643, 1900, 2353
HincII	GTYRAC	5 cut(s)	724, 1372, 1396, 3492, 4795
HindII	GTYRAC	5 cut(s)	724, 1372, 1396, 3492, 4795
HindIII	AAGCTT	5 cut(s)	842, 1776, 3208, 4076, 4083
HpaII	CCGG	6 cut(s)	138, 183, 227, 1225, 1468, 3608
Hpy99I	CGWCG	3 cut(s)	25, 401, 1953
HpyCH4IV	ACGT	7 cut(s)	311, 396, 1706, 2146, 2745, 3719, 4767
HpyF10VI	GCNNNNNNNGC	7 cut(s)	192, 484, 573, 641, 2690, 4460, 4810
HpySE526I	ACGT	7 cut(s)	311, 396, 1706, 2146, 2745, 3719, 4767
Hsp92I	GRCGYC	3 cut(s)	26, 396, 2745
HspAI	GCGC	3 cut(s)	1643, 1900, 2353
KflI	GGGWCCC	1 cut(s)	680
KpnI	GGTACC	1 cut(s)	3082
LmnI	GCTCC	6 cut(s)	517, 1003, 1418, 2410, 3412, 4319
MabI	ACCWGGT	1 cut(s)	672
MaeI	CTAG	8 cut(s)	432, 1332, 2100, 2630, 2882, 3429, 3662, 3845
MaeII	ACGT	7 cut(s)	311, 396, 1706, 2146, 2745, 3719, 4767
MfeI	CAATTG	5 cut(s)	168, 571, 1389, 2642, 4218
MflI	RGATCY	9 cut(s)	808, 1180, 1282, 1538, 2626, 4138, 4496, 4564, 4986
MhlI	GDGCHC	3 cut(s)	2415, 3897, 4351
MlsI	TGGCCA	1 cut(s)	2840
MluNI	TGGCCA	1 cut(s)	2840
MlyI	GAGTC	7 cut(s)	419, 1070, 2464, 3221, 3598, 4825, 4889
MmeI	TCCRAC	6 cut(s)	44, 1104, 2760, 2872, 3849, 4716
Mox20I	TGGCCA	1 cut(s)	2840
Mph1103I	ATGCAT	1 cut(s)	2487
MroXI	GAANNNNTTC	3 cut(s)	3263, 3512, 4400
MscI	TGGCCA	1 cut(s)	2840
MslI	CAYNNNNRTG	3 cut(s)	1130, 2540, 4839
Msp20I	TGGCCA	1 cut(s)	2840
MspA1I	CMGCKG	1 cut(s)	1824
MspI	CCGG	6 cut(s)	138, 183, 227, 1225, 1468, 3608
MspR9I	CCNGG	9 cut(s)	183, 227, 228, 674, 1185, 1416, 3233, 3444, 4738
MunI	CAATTG	5 cut(s)	168, 571, 1389, 2642, 4218
Mva1269I	GAATGC	1 cut(s)	1390
MvaI	CCWGG	6 cut(s)	674, 1185, 1416, 3233, 3444, 4738
MvnI	CGCG	2 cut(s)	217, 2355
MwoI	GCNNNNNNNGC	7 cut(s)	192, 484, 573, 641, 2690, 4460, 4810
NciI	CCSGG	3 cut(s)	183, 227, 228
NcoI	CCATGG	1 cut(s)	2939
NmeAIII	GCCGAG	2 cut(s)	141, 2550
NmuCI	GTSAC	8 cut(s)	392, 464, 470, 920, 1574, 3544, 3928, 4532
NsiI	ATGCAT	1 cut(s)	2487
NspI	RCATGY	7 cut(s)	204, 353, 482, 1649, 2499, 3621, 4706
OliI	CACNNNNGTG	1 cut(s)	4839
PaeI	GCATGC	1 cut(s)	482
PaeR7I	CTCGAG	2 cut(s)	1767, 4981
PagI	TCATGA	1 cut(s)	3612
PceI	AGGCCT	1 cut(s)	1218
PciI	ACATGT	4 cut(s)	200, 349, 2495, 3617
PcsI	WCGNNNNNNNCGW	4 cut(s)	1579, 3285, 4889, 4979
PctI	GAATGC	1 cut(s)	1390
PdmI	GAANNNNTTC	3 cut(s)	3263, 3512, 4400
PfeI	GAWTC	6 cut(s)	419, 586, 992, 1846, 2678, 3555
PflFI	GACNNNGTC	2 cut(s)	25, 1856
PfoI	TCCNGGA	2 cut(s)	1183, 1414
PleI	GAGTC	7 cut(s)	419, 1069, 2463, 3221, 3598, 4825, 4888
PmaCI	CACGTG	1 cut(s)	2147
PmlI	CACGTG	1 cut(s)	2147
PpsI	GAGTC	7 cut(s)	419, 1069, 2463, 3221, 3598, 4825, 4888
Ppu21I	YACGTR	2 cut(s)	1707, 2147
PpuMI	RGGWCCY	1 cut(s)	680
PscI	ACATGT	4 cut(s)	200, 349, 2495, 3617
PshAI	GACNNNNGTC	1 cut(s)	3608
PshBI	ATTAAT	1 cut(s)	2330
PsiI	TTATAA	2 cut(s)	267, 3765
Psp124BI	GAGCTC	2 cut(s)	2415, 4351
Psp1406I	AACGTT	1 cut(s)	4767
Psp5II	RGGWCCY	1 cut(s)	680
Psp6I	CCWGG	6 cut(s)	672, 1183, 1414, 3231, 3442, 4736
PspCI	CACGTG	1 cut(s)	2147
PspEI	GGTNACC	1 cut(s)	94
PspFI	CCCAGC	1 cut(s)	1902
PspGI	CCWGG	6 cut(s)	672, 1183, 1414, 3231, 3442, 4736
PspPI	GGNCC	6 cut(s)	164, 680, 1400, 1852, 2395, 4414
PspPPI	RGGWCCY	1 cut(s)	680
PspXI	VCTCGAGB	1 cut(s)	4981
PstI	CTGCAG	2 cut(s)	2434, 4623
PstNI	CAGNNNCTG	2 cut(s)	2120, 2429
PsuI	RGATCY	9 cut(s)	808, 1180, 1282, 1538, 2626, 4138, 4496, 4564, 4986
PsyI	GACNNNGTC	2 cut(s)	25, 1856
RsaI	GTAC	7 cut(s)	948, 1124, 1139, 1705, 1709, 3080, 4645
RsaNI	GTAC	7 cut(s)	947, 1123, 1138, 1704, 1708, 3079, 4644
RseI	CAYNNNNRTG	3 cut(s)	1130, 2540, 4839
SacI	GAGCTC	2 cut(s)	2415, 4351
Sau96I	GGNCC	6 cut(s)	164, 680, 1400, 1852, 2395, 4414
SchI	GAGTC	7 cut(s)	419, 1070, 2464, 3221, 3598, 4825, 4889
ScrFI	CCNGG	9 cut(s)	183, 227, 228, 674, 1185, 1416, 3233, 3444, 4738
SduI	GDGCHC	3 cut(s)	2415, 3897, 4351
SexAI	ACCWGGT	1 cut(s)	672
SfcI	CTRYAG	4 cut(s)	2121, 2198, 2430, 4619
Sfr274I	CTCGAG	2 cut(s)	1767, 4981
SinI	GGWCC	5 cut(s)	680, 1400, 1852, 2395, 4414
SlaI	CTCGAG	2 cut(s)	1767, 4981
SmaI	CCCGGG	1 cut(s)	228
SmiMI	CAYNNNNRTG	3 cut(s)	1130, 2540, 4839
SmlI	CTYRAG	5 cut(s)	1202, 1767, 3107, 3320, 4981
SmoI	CTYRAG	5 cut(s)	1202, 1767, 3107, 3320, 4981
SnaBI	TACGTA	1 cut(s)	1707
SphI	GCATGC	1 cut(s)	482
SseBI	AGGCCT	1 cut(s)	1218
SsiI	CCGC	6 cut(s)	195, 1178, 1824, 2111, 2355, 4734
SspI	AATATT	3 cut(s)	2439, 3973, 4383
SspMI	CTAG	8 cut(s)	432, 1332, 2100, 2630, 2882, 3429, 3662, 3845
SstI	GAGCTC	2 cut(s)	2415, 4351
StuI	AGGCCT	1 cut(s)	1218
StyD4I	CCNGG	9 cut(s)	181, 225, 226, 672, 1183, 1414, 3231, 3442, 4736
StyI	CCWWGG	6 cut(s)	898, 981, 2685, 2694, 2939, 4593
TaiI	ACGT	7 cut(s)	314, 399, 1709, 2149, 2748, 3722, 4770
TaqII	GACCGA	2 cut(s)	1254, 1941
TatI	WGTACW	3 cut(s)	946, 1122, 1137
TauI	GCSGC	1 cut(s)	4736
TfiI	GAWTC	6 cut(s)	419, 586, 992, 1846, 2678, 3555
TseFI	GTSAC	8 cut(s)	392, 464, 470, 920, 1574, 3544, 3928, 4532
Tsp45I	GTSAC	8 cut(s)	392, 464, 470, 920, 1574, 3544, 3928, 4532
TspMI	CCCGGG	1 cut(s)	226
Tth111I	GACNNNGTC	2 cut(s)	25, 1856
VpaK11BI	GGWCC	5 cut(s)	680, 1400, 1852, 2395, 4414
VspI	ATTAAT	1 cut(s)	2330
XagI	CCTNNNNNAGG	1 cut(s)	2698
XapI	RAATTY	9 cut(s)	965, 1311, 1761, 1910, 2180, 2657, 3221, 3263, 4424
XceI	RCATGY	7 cut(s)	204, 353, 482, 1649, 2499, 3621, 4706
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2052
XhoI	CTCGAG	2 cut(s)	1767, 4981
XmaI	CCCGGG	1 cut(s)	226
XmnI	GAANNNNTTC	3 cut(s)	3263, 3512, 4400
XspI	CTAG	8 cut(s)	432, 1332, 2100, 2630, 2882, 3429, 3662, 3845
ZraI	GACGTC	2 cut(s)	397, 2746
Zsp2I	ATGCAT	1 cut(s)	2487

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.