Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0005424

Physical Location & Seq

Genomic Coordinates Reverse (-)

6888066 .. 6905811

Gene Structure 17746 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4860 bp

ATGGGGACCGGACGGAGGCAGTTGAAGATTATGCTATGGAAGAATTGGCTTCTCAAAGTTCGTCACCCTTTCGTCACCTGCGCTGAGATATTACTTCCTACAGTGGTGATGTTGTTGTTAATAGCTGTGAGGATGCATGTTGATACACAGATTCATCCAGCGCAGCCGTATATCCGGAATGGGATGTTTGTGGAAGTGGGCAAAGGAATGTCACCTAATTTTGAACAAGTATTGGAATTACTGTTGAATAAGGAAGAGTTTCTGGCTTTTGCACCAGATACGGAAGAAACAAGGTCAATGATTAACATAATGTCGGTTAAATTTCCCCTCTTGAAGCGTGTTTCTAGAGTTTACAAAGATGAACAGGAGCTGGAAACCTACATACGCTCAGATCTCTATGGTACCTGCAATCAAATCTTGAATTGCTCGAATCCAAAAATCAAGGGAGCAGTTGTATTTCATGATCAAGGTCCTCAATCATTTGATTATAGCATACGTCTTAATCACACATGGGCTTTCTCGGGATTTCCTGATGTCAAATCCATCATGGACACGAATGGCCCCTACCTGAATGACTTGGAACTTGGTGTAAATGCTGTTCCGACCATGCAATACAGCGCTAGTGGATTTTTAACTCTCCAGCAAGTACTGGATTCATTTATTATTTTTGCGGCCCAACAATCTGACACTAAAGATATAGAACTTCCTTCATCGCTGTCATTTGGTGAACCTTCCTTTCTAAACGGTCCATGGATGCATTACAGCCCATCAAACATTCGAATAGTCCCGTTTCCAACTCGTGAATACACTGATGATGAGTTCCAGTCCATCATAAAAAGTGTGATGGGTGTATTGTATCTTCTGGGATTCCTCTACCCAATTTCTCGGCTGATCAGCTATTCGGTGTTTGAGAAGGAACAGAAGATAAAAGAAGGTCTTTATATGATGGGTCTAAAAGACGGGATATTCCATCTCTCATGGTTCATTACATATGCTTTGCAGTTCGCTATTTCTTCTGCAATCATTACAGTCTCCACTATGGATAACCTTTTCAAATATAGTGATAAGTCAGTTGTGTTCATTTACTTCTTCTTCTTCGGACTGAGCGCGATTATGCTGTCATTTTTAATCTCGACATGCTTCACACGAGCAAAGACAGCTGTTGCTGTTGGGACGCTTGCTTTTCTGGGTGCTTTTTTCCCTTACTACTCTGTCAATGATGAGGCTGTCCCTATGATATTAAAGGTTGTTGCTTCTCTGCTTTCGCCCACGGCATTTGCTCTAGGGTCAATTAACTTTGCTGATTATGAGCGTGCTCATGTTGGCCTTCGTTGGAGCAACATATGGCGGGCATCGTCTGAAGTGAATTTTCTGGTCTGTCTACTGATGATGTTGCTTGATGCATTGCTCTACTGTTTAATTGGTCTTTATCTGGACAAGGTCCTTCCGAGGGAGAATGGAATTAGATATCCATGGAACTTCATTTTTCAGAAGTGCTTCTGGAAAAATCCAAGCATTAAGGAACTTCATAATCACAATTCCAGTCTAGAAGTTAATAGCCATGACAAGGACTGCAAGAAGGCAAGTATTTCTGGGAAAGATAATGCCAGAGCATCTGTGGAAGCGATAACCTTTGACATGAAACAACAAGAGCTTGACCATAGATGCATCCAAATAAGAAATCTGCATAAGGTTTATGCCAGTAAAAAGGGGAAATGCTGTGCTGTTAACTCATTAGAGCTCACCATGTATGAAAATCAGATTCTTGCTCTTCTGGGACACAACGGGGCTGGTAAAAGCACAACAATATCAATGCTTGTTGGCCTTCTTCGCCCTACTTCAGGGGATGCTTTGGTTTTCGGGAAGAATATTGTTACAGACATGGATGAGATACGAAAGGAATTGGGGGTGTGTCCTCAAAATGATATCCTTTTTCCAGAATTGACAGTGAGGGAACATTTGGAAATATTTGCTATACTGAAAGGTGTGCAGGAAGATCTTCTGAATGGCGCTGTTGTTGATATGGTTGATCAAGTAGGTTTGGCTGATAAAATGAACTCTGCTGTGAGGGCTCTATCTGGAGGCATGAAGAGGAAGTTGTCTCTTGGAATTGCACTGATAGGAAACAGTAAGGTTATAATTTTGGATGAACCTACTAGTGGAATGGATCCATACTCCATGCGCTTGACATGGCAGCTAATAAAAAAAATCAGAAAGGGAAGGATAGTGTTACTGACAACGCACTCAATGGATGAAGCTGAAGTACTTGGAGATCGTATAGCCATCATGGCTAATGGTTCCTTGAAATGTTGCGGAAGTTCCCTTTTCTTGAAGCGTCAGTATGGGGTTGGTTATACTTTAACCTTGGTGAAATCGACACCTACTGCCTGTGTAGCTTCTGACATTGTTTACCGCCATATCCCATCAGCAACATGTGTGAGCGAGGTAGGAACGGAGATTTCCTTTAAGCTTCCTTTGGCATCCGCATCATCCTTTGAATGTATGTTTAGGGAAATTGAGAATTGCATGAACCGATCTACGTCAAACTCAGAAACAAGTTCTGGCGAGGAGAAGGACTATTTGGGCATTGAAAGTTATGGAATATCTGTAACAACTTTGGAGGAGGTATTCTTGAGGGTTGCAGGATGCGACTATGCTGAAGCTGCTAGTTTTGAACGGAAGACTGGTCAACGGTGTCTTGATTCTTTGATTTCTCAGTCTTCTCATGATTCTGCTCCCAAAAAGATCTCTGAGTCAAAAAAATCCTTTGGATATTATAAAGAGATTCTTGGGTTCTTATTTAGAATAGTGGGAAGAGCCTGTGGTTTGGTTGTCGCTACAGTTTTAAGTTTCTTAAACTTTGTTGGTGGTCATTGCTGTAGCTGTTGTTTCATTTCGAGGTCAACTTTTTGGCGACATTCTAAAGCATTGCTCACAAAGAGGGCGATATCTGCTCGCAGAGACCGGAAAACAATTGTTTTCCAGCTTGTTATACCTGCAGTTTTCTTATTTTTTGGTCTTCTGTTTCTCAAGCTCAAGCCACATCCTGATCAGCAGTCTGTTACATTTACAACTTCATATTTTAATCCACTATTGCGAGGTGGTGGTGGTGGTGGTCCAATCCCTTTTAATCTGTCCTTGCCTATTGCAAAGGAGGTTGCACAGTATGTTAAAGGGGGTTGGATACAAGAATTCAGACCTAGTGCATATAGATTCCCTAATTCAGATAAGATATTGGATGATGCAGTTGAGGCAGCGGGGCCAACCCTGGGACCTGTTTTACTTTCAATGAGCGAATTTTTAATGTCTAGCTTTAACGAATCTTACCAGTCAAGGTATGGGGCAATTTTGATGGATGATCAGAATGATGATGGAAGTTTAGGGTACACTGTGCTACACAACAGTTCCTGTCAGCATGCTGCCCCAACCTTTATCAATTTAATGAATGCTGCAATTCTTAGGCTTGCTGCTCGTAATAAGAATATGACAATCCAAACGCGTAATCACCCTCTGCCAATGACAAATAGCCAACATCTGCAACGTCATGATTTGGATGCATTCTCAGCTGCAGTTATTGTTAGTATTGCATTTTCATTCATTCCTGCATCATTTGCCGTTCCTATTGTGAAGGAGCGTGAAGTAAAAGCCAAACACCAACAACTGATTAGTGGGGTTTCTATTCTTTCATATTGGGCTTCTACTTTCATATGGGACTTCATCAGCTTCCTTTTTCCTTCATCGTTTGCAATAATTCTTTTTTACATCTTTGGTCTGGAGCAGTTCATTGGAAGCGGATATTTATTGTCAACAGTTGTCATGTTTTTAGCGTATGGATTGGCAATTGCATCATCCACATATTGCCTTACATTTTTCTTCTCTGACCACAGTATGGCTCAGAATGTGGTTCTCTTGGTCCACTTCTTTACGGGACTCATTCTTATGGTTATCTCATTCATCATGGGGCTTATAAAGACAACAGCAAGTGCAAATTCTTTTCTTAAGATCTTTTTCAGATTATCTCCAGGGTTTTGCTTTGCTGATGGACTTGCGTCACTGGCTCTTCTCCGGCAAGATATGAAAGATAAATCCAGCAATCAGGCCCTTGATTGGAACGTTACTGGTGGCTCAATTTGTTATCTTGGTATTGAGAGCGTTTGTTACTTCCTTTTGACACTTGGGCTTGAACTCTTACTTTCTAACAAGTGGACACTAGCCACACTGAAGGAATGTTGGAACAAAATTAGAAGCATCGAACATGGGACCCCAAGTTATCTAGAACCCCTTCTGAAGAGTTCCTCAGATGTTACTCTTGACCTTGATGAAGATATCGATGTAAAGAGAGAGAGAACTAGGGTGCTTTCAGGTTCCATTGACAATGCCATAATTTATTTGCGGAATCTTTGGAAGGTGTTTCCAGGAGGAAAGCATCATAGTGCAAAAATCGCGGTACATTCATTGACTTTCTCAGTTCAGGAAGGAGAGTGTTTTGGTTTTTTAGGAACAAATGGAGCTGGAAAGACCACAACTCTGTCAATGCTGACTGGAGAGGAATCTCCAACTGATGGGACTGCTTATATATTTGGAAGGGATATATGCTCTAATCCAAAGGCTGCTCGTCGACATATCGGATTTTGCCCACAGTTTGATGCTTTGTTGGAATTCTTAACTGTTAAAGAGCATCTTGAGCTTTATGCCACAATAAAAGGAGTACCAGATCACCGGTTGGATGATGTTGTAATGGAAAAGTTAATGGAGTTTGACTTGTTAAAGCATGCCAACAAACCATCATTTTCTCTCAGTGGGGGAAACAAGCGTAAATTATCTGTCGCAATTGCGATGATTGGATCGATCCTCCTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006869 GO:0008150 GO:0010876 GO:0015399 GO:0015405 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0031090 GO:0033036 GO:0042623 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko02010 map02010

Pfam Domains

Protein Families

ABC2_membrane_3 ABC_tran

Protein Analysis

1620

Amino Acids

180.57

Weight (kDa)

8.09

Isoelectric Point (pI)

37.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC2_membrane_3	PF12698	191 - 477	4.9e-20	ABC-2 family transporter protein
ABC_tran	PF00005	577 - 720	4.8e-31	ABC transporter
ABC2_membrane_3	PF12698	990 - 1392	2.8e-38	ABC-2 family transporter protein
ABC_tran	PF00005	1484 - 1614	4.5e-21	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005424)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41700 AT2G41700
fragaria_vesca	FvH4_6g24100 FvH4_6g24100
malus_domestica	MD12G1079400.v1.1 MD14G1075700.v1.1
prunus_persica	Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1 Prupe.7G058100_v2.0.a1
pyrus_communis	pycom12g06660
rosa_chinensis	RchiOBHm_Chr3g0482761
rosa_laevigata	RLG00000023331
rosa_multiflora	Rmu_co8213162.1_g000001 Rmu_sc0001542.1_g000002 Rmu_sc0021223.1_g000001
rosa_roxburghii	Rroxscaffold_6G00398990
rosa_rugosa	Rorug03G0198800 Rorug03G0198900
rosa_samantha	Rh3AG249700 Rh3BG285700 Rh3CG283300 Rh3DG279300
rosa_wichuraiana	Rw3G022560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	2138, 2778, 3968
AarI	CACCTGC	1 cut(s)	86
Acc36I	ACCTGC	3 cut(s)	86, 413, 3004
Acc65I	GGTACC	1 cut(s)	401
AccB1I	GGYRCC	1 cut(s)	401
AccB7I	CCANNNNNTGG	2 cut(s)	3889, 4562
AccI	GTMKAC	2 cut(s)	1381, 4618
AccII	CGCG	3 cut(s)	1109, 3499, 4445
AccIII	TCCGGA	1 cut(s)	174
AciI	CCGC	9 cut(s)	671, 1348, 2313, 2413, 2484, 3257, 3792, 4393, 4445
AclI	AACGTT	1 cut(s)	4113
AclWI	GGATC	4 cut(s)	2162, 2175, 4843, 4852
AcsI	RAATTY	6 cut(s)	320, 1366, 3191, 3296, 3988, 4658
AcuI	CTGAAG	6 cut(s)	1380, 1824, 2280, 2679, 4241, 4307
AfaI	GTAC	6 cut(s)	403, 648, 2265, 3386, 4449, 4710
AfeI	AGCGCT	1 cut(s)	619
AfiI	CCNNNNNNNGG	9 cut(s)	15, 1450, 1567, 1841, 2159, 3269, 3889, 4107, 4562
AflII	CTTAAG	1 cut(s)	3998
AflIII	ACRYGT	2 cut(s)	2432, 3497
AgeI	ACCGGT	1 cut(s)	4719
AhlI	ACTAGT	1 cut(s)	2156
AjnI	CCWGG	3 cut(s)	3267, 4021, 4414
AjuI	GAANNNNNNNTTGG	2 cut(s)	2564, 2596
Alw21I	GWGCWC	2 cut(s)	1318, 1743
Alw26I	GTCTC	3 cut(s)	1036, 2106, 2955
AlwI	GGATC	4 cut(s)	2162, 2175, 4843, 4852
AlwNI	CAGNNNCTG	2 cut(s)	370, 3408
Ama87I	CYCGRG	1 cut(s)	520
Aor13HI	TCCGGA	1 cut(s)	174
Aor51HI	AGCGCT	1 cut(s)	619
AoxI	GGCC	6 cut(s)	559, 672, 1324, 1822, 3260, 4098
ApeKI	GCWGC	9 cut(s)	163, 2194, 2663, 3254, 3419, 3449, 3467, 3566, 4610
ApoI	RAATTY	6 cut(s)	320, 1366, 3191, 3296, 3988, 4658
AsiGI	ACCGGT	1 cut(s)	4719
Asp700I	GAANNNNTTC	4 cut(s)	258, 1496, 1998, 4281
Asp718I	GGTACC	1 cut(s)	401
AspLEI	GCGC	6 cut(s)	83, 163, 620, 1109, 2012, 2184
AsuHPI	GGTGA	9 cut(s)	56, 67, 118, 204, 737, 1735, 2380, 3497, 4709
AsuII	TTCGAA	1 cut(s)	778
AvaI	CYCGRG	1 cut(s)	520
AvaII	GGWCC	8 cut(s)	6, 470, 746, 1441, 3116, 3272, 3913, 4260
BamHI	GGATCC	1 cut(s)	2167
BanI	GGYRCC	1 cut(s)	401
BanII	GRGCYC	2 cut(s)	1743, 2074
BarI	GAAGNNNNNNTAC	4 cut(s)	843, 875, 2247, 2279
BauI	CACGAG	2 cut(s)	798, 1146
BbsI	GAAGAC	3 cut(s)	2687, 2712, 3011
Bbv12I	GWGCWC	2 cut(s)	1318, 1743
BbvI	GCAGC	9 cut(s)	175, 2206, 2650, 3266, 3406, 3436, 3454, 3553, 4597
BceAI	ACGGC	3 cut(s)	151, 1287, 3599
BciT130I	CCWGG	3 cut(s)	3269, 4023, 4416
BciVI	GTATCC	1 cut(s)	3177
BclI	TGATCA	5 cut(s)	463, 891, 2029, 3049, 3358
BcoDI	GTCTC	3 cut(s)	1036, 2106, 2955
BcuI	ACTAGT	1 cut(s)	2156
BfmI	CTRYAG	6 cut(s)	99, 2838, 2878, 2997, 3567, 4856
BfoI	RGCGCY	2 cut(s)	621, 2013
BfrI	CTTAAG	1 cut(s)	3998
BfuAI	ACCTGC	3 cut(s)	86, 413, 3004
BfuI	GTATCC	1 cut(s)	3177
BglI	GCCNNNNNGGC	1 cut(s)	2288
BglII	AGATCT	4 cut(s)	391, 1996, 2745, 4002
BmcAI	AGTACT	2 cut(s)	648, 2265
Bme1390I	CCNGG	3 cut(s)	3269, 4023, 4416
Bme18I	GGWCC	8 cut(s)	6, 470, 746, 1441, 3116, 3272, 3913, 4260
BmeT110I	CYCGRG	1 cut(s)	520
BmrFI	CCNGG	3 cut(s)	3269, 4023, 4416
BoxI	GACNNNNGTC	1 cut(s)	4048
BpiI	GAAGAC	3 cut(s)	2687, 2712, 3011
BpmI	CTGGAG	5 cut(s)	623, 2100, 3794, 4005, 4563
Bpu14I	TTCGAA	1 cut(s)	778
BpuEI	CTTGAG	4 cut(s)	2653, 3014, 3020, 4703
Bsa29I	ATCGAT	2 cut(s)	4329, 4847
BsaI	GGTCTC	1 cut(s)	2955
BsaJI	CCNNGG	8 cut(s)	749, 1269, 1448, 1472, 2364, 3267, 3268, 4022
BsaWI	WCCGGW	4 cut(s)	8, 174, 2964, 4719
BsaXI	ACNNNNNCTCC	2 cut(s)	438, 468
Bsc4I	CCNNNNNNNGG	9 cut(s)	15, 1450, 1567, 1841, 2159, 3269, 3889, 4107, 4562
Bse118I	RCCGGY	1 cut(s)	4719
Bse1I	ACTGG	9 cut(s)	654, 823, 1542, 1701, 2689, 3328, 4059, 4123, 4546
Bse3DI	GCAATG	3 cut(s)	1403, 2872, 2927
BseAI	TCCGGA	1 cut(s)	174
BseBI	CCWGG	3 cut(s)	3269, 4023, 4416
BseCI	ATCGAT	2 cut(s)	4329, 4847
BseDI	CCNNGG	8 cut(s)	749, 1269, 1448, 1472, 2364, 3267, 3268, 4022
BseLI	CCNNNNNNNGG	9 cut(s)	15, 1450, 1567, 1841, 2159, 3269, 3889, 4107, 4562
BseMI	GCAATG	3 cut(s)	1403, 2872, 2927
BseNI	ACTGG	9 cut(s)	654, 823, 1542, 1701, 2689, 3328, 4059, 4123, 4546
BseRI	GAGGAG	2 cut(s)	2582, 2636
BseXI	GCAGC	9 cut(s)	175, 2206, 2650, 3266, 3406, 3436, 3454, 3553, 4597
BsgI	GTGCAG	1 cut(s)	2009
Bsh1236I	CGCG	3 cut(s)	1109, 3499, 4445
BshFI	GGCC	6 cut(s)	561, 674, 1326, 1824, 3262, 4100
BshNI	GGYRCC	1 cut(s)	401
BshTI	ACCGGT	1 cut(s)	4719
BshVI	ATCGAT	2 cut(s)	4329, 4847
BsiHKAI	GWGCWC	2 cut(s)	1318, 1743
BsiHKCI	CYCGRG	1 cut(s)	520
BsiSI	CCGG	5 cut(s)	9, 175, 2965, 4066, 4720
BslI	CCNNNNNNNGG	9 cut(s)	15, 1450, 1567, 1841, 2159, 3269, 3889, 4107, 4562
BsmAI	GTCTC	3 cut(s)	1036, 2106, 2955
BsmI	GAATGC	2 cut(s)	3451, 3557
BsnI	GGCC	6 cut(s)	561, 674, 1326, 1824, 3262, 4100
Bso31I	GGTCTC	1 cut(s)	2955
BsoBI	CYCGRG	1 cut(s)	520
Bsp119I	TTCGAA	1 cut(s)	778
Bsp1286I	GDGCHC	3 cut(s)	1318, 1743, 2074
Bsp13I	TCCGGA	1 cut(s)	174
Bsp19I	CCATGG	2 cut(s)	749, 1472
BspACI	CCGC	9 cut(s)	671, 1348, 2313, 2413, 2484, 3257, 3792, 4393, 4445
BspANI	GGCC	6 cut(s)	561, 674, 1326, 1824, 3262, 4100
BspDI	ATCGAT	2 cut(s)	4329, 4847
BspEI	TCCGGA	1 cut(s)	174
BspFNI	CGCG	3 cut(s)	1109, 3499, 4445
BspHI	TCATGA	3 cut(s)	460, 2725, 3544
BspMAI	CTGCAG	2 cut(s)	3001, 3571
BspMI	ACCTGC	3 cut(s)	86, 413, 3004
BspPI	GGATC	4 cut(s)	2162, 2175, 4843, 4852
BspQI	GCTCTTC	3 cut(s)	1776, 2809, 4065
BspT104I	TTCGAA	1 cut(s)	778
BspT107I	GGYRCC	1 cut(s)	401
BspTI	CTTAAG	1 cut(s)	3998
BspTNI	GGTCTC	1 cut(s)	2955
BsrDI	GCAATG	3 cut(s)	1403, 2872, 2927
BsrFI	RCCGGY	1 cut(s)	4719
BsrI	ACTGG	9 cut(s)	654, 823, 1542, 1701, 2689, 3328, 4059, 4123, 4546
BssAI	RCCGGY	1 cut(s)	4719
BssECI	CCNNGG	8 cut(s)	749, 1269, 1448, 1472, 2364, 3267, 3268, 4022
BssSI	CACGAG	2 cut(s)	798, 1146
BssT1I	CCWWGG	3 cut(s)	749, 1472, 2364
Bst2BI	CACGAG	2 cut(s)	798, 1146
Bst2UI	CCWGG	3 cut(s)	3269, 4023, 4416
Bst6I	CTCTTC	6 cut(s)	249, 1776, 2084, 2809, 4065, 4283
BstAFI	CTTAAG	1 cut(s)	3998
BstAPI	GCANNNNNTGC	1 cut(s)	3611
BstBI	TTCGAA	1 cut(s)	778
BstC8I	GCNNGC	7 cut(s)	1179, 1314, 1350, 2956, 3417, 3465, 4773
BstDSI	CCRYGG	3 cut(s)	749, 1269, 1472
BstENI	CCTNNNNNAGG	1 cut(s)	1839
BstFNI	CGCG	3 cut(s)	1109, 3499, 4445
BstH2I	RGCGCY	2 cut(s)	621, 2013
BstHHI	GCGC	6 cut(s)	83, 163, 620, 1109, 2012, 2184
BstMAI	GTCTC	3 cut(s)	1036, 2106, 2955
BstNI	CCWGG	3 cut(s)	3269, 4023, 4416
BstNSI	RCATGY	5 cut(s)	140, 1140, 2436, 3419, 4775
BstPAI	GACNNNNGTC	1 cut(s)	4048
BstSCI	CCNGG	3 cut(s)	3267, 4021, 4414
BstSFI	CTRYAG	6 cut(s)	99, 2838, 2878, 2997, 3567, 4856
BstUI	CGCG	3 cut(s)	1109, 3499, 4445
BstV1I	GCAGC	9 cut(s)	175, 2206, 2650, 3266, 3406, 3436, 3454, 3553, 4597
BstV2I	GAAGAC	3 cut(s)	2687, 2712, 3011
BstX2I	RGATCY	5 cut(s)	391, 1996, 2167, 2745, 4002
BstYI	RGATCY	5 cut(s)	391, 1996, 2167, 2745, 4002
Bsu15I	ATCGAT	2 cut(s)	4329, 4847
BsuI	GTATCC	1 cut(s)	3177
BsuRI	GGCC	6 cut(s)	561, 674, 1326, 1824, 3262, 4100
BsuTUI	ATCGAT	2 cut(s)	4329, 4847
BtgI	CCRYGG	3 cut(s)	749, 1269, 1472
BtgZI	GCGATG	2 cut(s)	696, 4850
BtsIMutI	CAGTG	8 cut(s)	108, 807, 1953, 2114, 3387, 4052, 4217, 4804
BveI	ACCTGC	3 cut(s)	86, 413, 3004
Cac8I	GCNNGC	7 cut(s)	1179, 1314, 1350, 2956, 3417, 3465, 4773
CaiI	CAGNNNCTG	2 cut(s)	370, 3408
CciI	TCATGA	3 cut(s)	460, 2725, 3544
CfoI	GCGC	6 cut(s)	83, 163, 620, 1109, 2012, 2184
Cfr10I	RCCGGY	1 cut(s)	4719
ClaI	ATCGAT	2 cut(s)	4329, 4847
CseI	GACGC	3 cut(s)	1183, 2324, 4038
Csp6I	GTAC	6 cut(s)	402, 647, 2264, 3385, 4448, 4709
CspAI	ACCGGT	1 cut(s)	4719
CspCI	CAANNNNNGTGG	2 cut(s)	1258, 1293
CviQI	GTAC	6 cut(s)	402, 647, 2264, 3385, 4448, 4709
Eam1104I	CTCTTC	6 cut(s)	249, 1776, 2084, 2809, 4065, 4283
EarI	CTCTTC	6 cut(s)	249, 1776, 2084, 2809, 4065, 4283
Ecl136II	GAGCTC	1 cut(s)	1741
Eco130I	CCWWGG	3 cut(s)	749, 1472, 2364
Eco24I	GRGCYC	2 cut(s)	1743, 2074
Eco31I	GGTCTC	1 cut(s)	2955
Eco32I	GATATC	4 cut(s)	1469, 1927, 2949, 4327
Eco47I	GGWCC	8 cut(s)	6, 470, 746, 1441, 3116, 3272, 3913, 4260
Eco47III	AGCGCT	1 cut(s)	619
Eco53kI	GAGCTC	1 cut(s)	1741
Eco57I	CTGAAG	6 cut(s)	1380, 1824, 2280, 2679, 4241, 4307
Eco88I	CYCGRG	1 cut(s)	520
EcoICRI	GAGCTC	1 cut(s)	1741
EcoNI	CCTNNNNNAGG	1 cut(s)	1839
EcoO109I	RGGNCCY	5 cut(s)	470, 1441, 3272, 4099, 4260
EcoRI	GAATTC	2 cut(s)	3191, 4658
EcoRII	CCWGG	3 cut(s)	3267, 4021, 4414
EcoRV	GATATC	4 cut(s)	1469, 1927, 2949, 4327
EcoT14I	CCWWGG	3 cut(s)	749, 1472, 2364
EcoT22I	ATGCAT	5 cut(s)	138, 759, 1405, 1670, 3559
EcoT38I	GRGCYC	2 cut(s)	1743, 2074
ErhI	CCWWGG	3 cut(s)	749, 1472, 2364
FalI	AAGNNNNNCTT	6 cut(s)	921, 953, 2772, 2804, 4665, 4697
FauI	CCCGC	2 cut(s)	1341, 3250
FauNDI	CATATG	3 cut(s)	991, 1343, 3707
FbaI	TGATCA	5 cut(s)	463, 891, 2029, 3049, 3358
FblI	GTMKAC	2 cut(s)	1381, 4618
FriOI	GRGCYC	2 cut(s)	1743, 2074
GlaI	GCGC	6 cut(s)	82, 162, 619, 1108, 2011, 2183
GsuI	CTGGAG	5 cut(s)	623, 2100, 3794, 4005, 4563
HaeII	RGCGCY	2 cut(s)	621, 2013
HaeIII	GGCC	6 cut(s)	561, 674, 1326, 1824, 3262, 4100
HapII	CCGG	5 cut(s)	9, 175, 2965, 4066, 4720
HgaI	GACGC	3 cut(s)	1183, 2324, 4038
HhaI	GCGC	6 cut(s)	83, 163, 620, 1109, 2012, 2184
Hin6I	GCGC	6 cut(s)	81, 161, 618, 1107, 2010, 2182
HinP1I	GCGC	6 cut(s)	81, 161, 618, 1107, 2010, 2182
HincII	GTYRAC	5 cut(s)	1729, 2690, 2904, 3807, 4619
HindII	GTYRAC	5 cut(s)	1729, 2690, 2904, 3807, 4619
HindIII	AAGCTT	1 cut(s)	2468
HpaI	GTTAAC	1 cut(s)	1729
HpaII	CCGG	5 cut(s)	9, 175, 2965, 4066, 4720
HphI	GGTGA	9 cut(s)	56, 67, 118, 204, 737, 1735, 2380, 3497, 4709
Hpy99I	CGWCG	1 cut(s)	4620
HpyCH4IV	ACGT	4 cut(s)	496, 2540, 3541, 4113
HpySE526I	ACGT	4 cut(s)	496, 2540, 3541, 4113
HspAI	GCGC	6 cut(s)	81, 161, 618, 1107, 2010, 2182
KflI	GGGWCCC	1 cut(s)	4260
Kpn2I	TCCGGA	1 cut(s)	174
KpnI	GGTACC	1 cut(s)	405
Ksp22I	TGATCA	5 cut(s)	463, 891, 2029, 3049, 3358
KspAI	GTTAAC	1 cut(s)	1729
LguI	GCTCTTC	3 cut(s)	1776, 2809, 4065
LmnI	GCTCC	7 cut(s)	367, 446, 1335, 2740, 3631, 3775, 4508
Lsp1109I	GCAGC	9 cut(s)	175, 2206, 2650, 3266, 3406, 3436, 3454, 3553, 4597
MaeII	ACGT	4 cut(s)	496, 2540, 3541, 4113
MfeI	CAATTG	3 cut(s)	2973, 3840, 4830
MflI	RGATCY	5 cut(s)	391, 1996, 2167, 2745, 4002
MhlI	GDGCHC	3 cut(s)	1318, 1743, 2074
MluI	ACGCGT	1 cut(s)	3497
MlyI	GAGTC	2 cut(s)	2762, 3924
MmeI	TCCRAC	8 cut(s)	626, 818, 1313, 3161, 4211, 4580, 4635, 4704
Mph1103I	ATGCAT	5 cut(s)	138, 759, 1405, 1670, 3559
MroI	TCCGGA	1 cut(s)	174
MroXI	GAANNNNTTC	4 cut(s)	258, 1496, 1998, 4281
MslI	CAYNNNNRTG	2 cut(s)	3938, 4431
MspA1I	CMGCKG	3 cut(s)	1160, 3257, 3566
MspCI	CTTAAG	1 cut(s)	3998
MspI	CCGG	5 cut(s)	9, 175, 2965, 4066, 4720
MspR9I	CCNGG	3 cut(s)	3269, 4023, 4416
MunI	CAATTG	3 cut(s)	2973, 3840, 4830
Mva1269I	GAATGC	2 cut(s)	3451, 3557
MvaI	CCWGG	3 cut(s)	3269, 4023, 4416
MvnI	CGCG	3 cut(s)	1109, 3499, 4445
NcoI	CCATGG	2 cut(s)	749, 1472
NdeI	CATATG	3 cut(s)	991, 1343, 3707
NmeAIII	GCCGAG	1 cut(s)	865
NmuCI	GTSAC	4 cut(s)	62, 73, 210, 4050
NsiI	ATGCAT	5 cut(s)	138, 759, 1405, 1670, 3559
NspI	RCATGY	5 cut(s)	140, 1140, 2436, 3419, 4775
NspV	TTCGAA	1 cut(s)	778
PaeI	GCATGC	2 cut(s)	3419, 4775
PagI	TCATGA	3 cut(s)	460, 2725, 3544
PaqCI	CACCTGC	1 cut(s)	86
PasI	CCCWGGG	1 cut(s)	3268
PciI	ACATGT	1 cut(s)	2432
PciSI	GCTCTTC	3 cut(s)	1776, 2809, 4065
PctI	GAATGC	2 cut(s)	3451, 3557
PdmI	GAANNNNTTC	4 cut(s)	258, 1496, 1998, 4281
PflFI	GACNNNGTC	1 cut(s)	1439
PflMI	CCANNNNNTGG	2 cut(s)	3889, 4562
PfoI	TCCNGGA	1 cut(s)	4414
PinAI	ACCGGT	1 cut(s)	4719
PleI	GAGTC	2 cut(s)	2761, 3924
PpsI	GAGTC	2 cut(s)	2761, 3924
PpuMI	RGGWCCY	4 cut(s)	470, 1441, 3272, 4260
PscI	ACATGT	1 cut(s)	2432
PshAI	GACNNNNGTC	1 cut(s)	4048
PsiI	TTATAA	3 cut(s)	2138, 2778, 3968
Psp124BI	GAGCTC	1 cut(s)	1743
Psp1406I	AACGTT	1 cut(s)	4113
Psp5II	RGGWCCY	4 cut(s)	470, 1441, 3272, 4260
Psp6I	CCWGG	3 cut(s)	3267, 4021, 4414
PspGI	CCWGG	3 cut(s)	3267, 4021, 4414
PspPPI	RGGWCCY	4 cut(s)	470, 1441, 3272, 4260
PsrI	GAACNNNNNNTAC	2 cut(s)	582, 614
PstI	CTGCAG	2 cut(s)	3001, 3571
PstNI	CAGNNNCTG	2 cut(s)	370, 3408
PsuI	RGATCY	5 cut(s)	391, 1996, 2167, 2745, 4002
PsyI	GACNNNGTC	1 cut(s)	1439
PvuII	CAGCTG	2 cut(s)	1160, 3566
RsaI	GTAC	6 cut(s)	403, 648, 2265, 3386, 4449, 4710
RsaNI	GTAC	6 cut(s)	402, 647, 2264, 3385, 4448, 4709
RseI	CAYNNNNRTG	2 cut(s)	3938, 4431
SacI	GAGCTC	1 cut(s)	1743
SalI	GTCGAC	1 cut(s)	4617
SapI	GCTCTTC	3 cut(s)	1776, 2809, 4065
ScaI	AGTACT	2 cut(s)	648, 2265
SchI	GAGTC	2 cut(s)	2762, 3924
ScrFI	CCNGG	3 cut(s)	3269, 4023, 4416
SduI	GDGCHC	3 cut(s)	1318, 1743, 2074
SfcI	CTRYAG	6 cut(s)	99, 2838, 2878, 2997, 3567, 4856
SfuI	TTCGAA	1 cut(s)	778
SinI	GGWCC	8 cut(s)	6, 470, 746, 1441, 3116, 3272, 3913, 4260
SmiMI	CAYNNNNRTG	2 cut(s)	3938, 4431
SmlI	CTYRAG	5 cut(s)	2632, 3029, 3035, 3998, 4682
SmoI	CTYRAG	5 cut(s)	2632, 3029, 3035, 3998, 4682
SpeI	ACTAGT	1 cut(s)	2156
SphI	GCATGC	2 cut(s)	3419, 4775
SsiI	CCGC	9 cut(s)	671, 1348, 2313, 2413, 2484, 3257, 3792, 4393, 4445
SspI	AATATT	2 cut(s)	1870, 1968
SstI	GAGCTC	1 cut(s)	1743
StyD4I	CCNGG	3 cut(s)	3267, 4021, 4414
StyI	CCWWGG	3 cut(s)	749, 1472, 2364
TaiI	ACGT	4 cut(s)	499, 2543, 3544, 4116
TaqI	TCGA	9 cut(s)	428, 778, 1133, 2375, 2897, 4251, 4329, 4618, 4847
TatI	WGTACW	2 cut(s)	646, 2263
TauI	GCSGC	1 cut(s)	674
TscAI	CASTG	8 cut(s)	108, 814, 1953, 2121, 3394, 4059, 4224, 4804
TseFI	GTSAC	4 cut(s)	62, 73, 210, 4050
TseI	GCWGC	9 cut(s)	163, 2194, 2663, 3254, 3419, 3449, 3467, 3566, 4610
Tsp45I	GTSAC	4 cut(s)	62, 73, 210, 4050
TspGWI	ACGGA	4 cut(s)	28, 296, 2468, 2692
TspRI	CASTG	8 cut(s)	108, 814, 1953, 2121, 3394, 4059, 4224, 4804
Tth111I	GACNNNGTC	1 cut(s)	1439
Van91I	CCANNNNNTGG	2 cut(s)	3889, 4562
Vha464I	CTTAAG	1 cut(s)	3998
VpaK11BI	GGWCC	8 cut(s)	6, 470, 746, 1441, 3116, 3272, 3913, 4260
XagI	CCTNNNNNAGG	1 cut(s)	1839
XapI	RAATTY	6 cut(s)	320, 1366, 3191, 3296, 3988, 4658
XbaI	TCTAGA	3 cut(s)	344, 1546, 4273
XceI	RCATGY	5 cut(s)	140, 1140, 2436, 3419, 4775
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1615, 3335
XmiI	GTMKAC	2 cut(s)	1381, 4618
XmnI	GAANNNNTTC	4 cut(s)	258, 1496, 1998, 4281
ZrmI	AGTACT	2 cut(s)	648, 2265
Zsp2I	ATGCAT	5 cut(s)	138, 759, 1405, 1670, 3559

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.