pycom12g07070

Glucan endo-1,3-beta-glucosidase, basic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0006449
Physical Location & Seq
Forward (+)
7373842 .. 7375810
1969 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1038 bp
ATGGCTAAATCCAATTCTTCGGTTGGCAGAGGCTCTTCTGTCATTTCGATGATACTTCTACTTGGGCAACTGATGGCTAGCTTTCAAACAACAGGTGCTCAAATTGGTGTATGTTATGGAATGCTTGGCGACGACCTACCATCCCAAGCAGAAGTTATTACTCTCTTCAACCAAAATAATATCCATAGAATGAGACTATATAATCCAAATCCAGCCGCTCTGGAGGCCCTTAGAGGCTCTAACATTGAGCTCATGTTAGGCATACCGGATGAAAACCTTCAAAACCTTGCCACAAGCCAAGATAATGCAAATACATGGGTTCAAAACAACGTGAGAAAGTATGACAATGTAAAATTCAAATATCTTGCGGTAGGAAATGAAATCAAGCTCTCGGACTCATTAGCACAATTTCTTGTCCCGGCAATGCGAAACATTCAAAATGCAATTTCTAGTGCTAGTCTTGGAAACCAAATCAAAGTTTCCACTGCTTTTGACACTACAGTACTTGGAGTGTCCTTTCCTCCATCAAAAGGCGTATTCAAGTCCGAATATAGAGCAGTTTTGGATCCCCTCATTGCTTTCCTCGTGGACAACTACTCTCCGCTACTCCTTAACATGTACCCTTATTTTGCTTACAGTGGCAGCAGTGGTGACGTTCGTCTTGATTATGCTCTTTTCACAGCTCCCTCAGTTGTAGTACAAGATGACAACCTCGGTTACAATAATCTTTTCGATGCAATTTTGGATAGTGCTTATGCAGCTCTTGAGAGGGCGGGTGCAGGGTCTTTGGCAATTGTTGTATCGGAGACTGGCTGGCCATCAGCTGGTGCAAATTCAACAACAATTGATAATGCAAGGACTTATAACTCAAATTTGATTAAACATGTGAAGAAAGGGACGCCGAGGAAGCCTGGAAAGCCCATAGAAACTTACATTTTCGCCATGTTTGATGAGAATAGAAAGACCCCAGAGCTTGAAAAACATTGGGGGCTGTTTTCCCCAAACAAACAGCCTAAATACCCAATTGATTTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_17

Protein Analysis

346

Amino Acids

37.77

Weight (kDa)

6.33

Isoelectric Point (pI)

31.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 35 - 344 6.6e-117 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 864
AccB7I CCANNNNNTGG 1 cut(s) 824
AccBSI CCGCTC 1 cut(s) 218
AciI CCGC 4 cut(s) 216, 368, 602, 773
AclWI GGATC 2 cut(s) 560, 573
AcoI YGGCCR 1 cut(s) 815
AcsI RAATTY 3 cut(s) 353, 832, 871
AcyI GRCGYC 1 cut(s) 899
AfaI GTAC 3 cut(s) 504, 620, 699
AfiI CCNNNNNNNGG 2 cut(s) 530, 824
AflIII ACRYGT 2 cut(s) 615, 883
AjnI CCWGG 1 cut(s) 910
AluBI AGCT 7 cut(s) 81, 250, 388, 683, 761, 824, 973
AluI AGCT 7 cut(s) 81, 250, 388, 683, 761, 824, 973
Alw21I GWGCWC 2 cut(s) 100, 252
Alw26I GTCTC 2 cut(s) 187, 800
AlwI GGATC 2 cut(s) 560, 573
AoxI GGCC 2 cut(s) 225, 815
ApeKI GCWGC 2 cut(s) 642, 758
ApoI RAATTY 3 cut(s) 353, 832, 871
Asp700I GAANNNNTTC 1 cut(s) 276
AspS9I GGNCC 1 cut(s) 226
AsuC2I CCSGG 1 cut(s) 419
AsuHPI GGTGA 1 cut(s) 662
AsuNHI GCTAGC 1 cut(s) 77
BalI TGGCCA 1 cut(s) 817
BamHI GGATCC 1 cut(s) 565
BanII GRGCYC 1 cut(s) 252
BauI CACGAG 1 cut(s) 584
Bbv12I GWGCWC 2 cut(s) 100, 252
BbvI GCAGC 2 cut(s) 654, 770
BccI CCATC 4 cut(s) 67, 148, 532, 826
BciT130I CCWGG 1 cut(s) 912
BcnI CCSGG 1 cut(s) 419
BcoDI GTCTC 2 cut(s) 187, 800
BfaI CTAG 3 cut(s) 78, 450, 456
BfmI CTRYAG 1 cut(s) 498
BisI GCNGC 3 cut(s) 216, 643, 759
BlsI GCNGC 3 cut(s) 217, 644, 760
BmcAI AGTACT 1 cut(s) 504
Bme1390I CCNGG 2 cut(s) 419, 912
BmgT120I GGNCC 1 cut(s) 226
BmiI GGNNCC 1 cut(s) 567
BmrFI CCNGG 2 cut(s) 419, 912
BmsI GCATC 1 cut(s) 724
BmtI GCTAGC 1 cut(s) 81
BoxI GACNNNNGTC 1 cut(s) 657
BpmI CTGGAG 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 785
BpuMI CCSGG 1 cut(s) 419
BsaHI GRCGYC 1 cut(s) 899
BsaJI CCNNGG 2 cut(s) 712, 902
BsaWI WCCGGW 1 cut(s) 265
Bsc4I CCNNNNNNNGG 2 cut(s) 530, 824
Bse1I ACTGG 1 cut(s) 814
Bse3DI GCAATG 2 cut(s) 429, 573
BseBI CCWGG 1 cut(s) 912
BseDI CCNNGG 2 cut(s) 712, 902
BseGI GGATG 2 cut(s) 140, 274
BseLI CCNNNNNNNGG 2 cut(s) 530, 824
BseMI GCAATG 2 cut(s) 429, 573
BseMII CTCAG 1 cut(s) 702
BseNI ACTGG 1 cut(s) 814
BseXI GCAGC 2 cut(s) 654, 770
BsgI GTGCAG 1 cut(s) 798
BshFI GGCC 2 cut(s) 227, 817
BsiHKAI GWGCWC 2 cut(s) 100, 252
BsiSI CCGG 2 cut(s) 266, 419
BslFI GGGAC 2 cut(s) 401, 910
BslI CCNNNNNNNGG 2 cut(s) 530, 824
BsmAI GTCTC 2 cut(s) 187, 800
BsmFI GGGAC 2 cut(s) 401, 910
BsmI GAATGC 1 cut(s) 126
BsnI GGCC 2 cut(s) 227, 817
Bsp1286I GDGCHC 2 cut(s) 100, 252
Bsp143I GATC 1 cut(s) 565
BspACI CCGC 4 cut(s) 216, 368, 602, 773
BspANI GGCC 2 cut(s) 227, 817
BspCNI CTCAG 1 cut(s) 701
BspLI GGNNCC 1 cut(s) 567
BspOI GCTAGC 1 cut(s) 81
BspPI GGATC 2 cut(s) 560, 573
BspQI GCTCTTC 1 cut(s) 40
BsrBI CCGCTC 1 cut(s) 218
BsrDI GCAATG 2 cut(s) 429, 573
BsrI ACTGG 1 cut(s) 814
BssECI CCNNGG 2 cut(s) 712, 902
BssMI GATC 1 cut(s) 565
BssNI GRCGYC 1 cut(s) 899
BssSI CACGAG 1 cut(s) 584
Bst2BI CACGAG 1 cut(s) 584
Bst2UI CCWGG 1 cut(s) 912
Bst4CI ACNGT 2 cut(s) 502, 638
Bst6I CTCTTC 2 cut(s) 40, 170
BstACI GRCGYC 1 cut(s) 899
BstC8I GCNNGC 2 cut(s) 79, 815
BstDEI CTNAG 2 cut(s) 230, 688
BstF5I GGATG 2 cut(s) 140, 274
BstKTI GATC 1 cut(s) 568
BstMAI GTCTC 2 cut(s) 187, 800
BstMBI GATC 1 cut(s) 565
BstMWI GCNNNNNNNGC 4 cut(s) 224, 758, 907, 916
BstNI CCWGG 1 cut(s) 912
BstNSI RCATGY 2 cut(s) 619, 887
BstPAI GACNNNNGTC 1 cut(s) 657
BstSCI CCNGG 2 cut(s) 417, 910
BstSFI CTRYAG 1 cut(s) 498
BstV1I GCAGC 2 cut(s) 654, 770
BstX2I RGATCY 1 cut(s) 565
BstYI RGATCY 1 cut(s) 565
BsuRI GGCC 2 cut(s) 227, 817
BtsCI GGATG 2 cut(s) 140, 274
BtsI GCAGTG 2 cut(s) 483, 652
BtsIMutI CAGTG 3 cut(s) 483, 643, 652
Cac8I GCNNGC 2 cut(s) 79, 815
Cfr13I GGNCC 1 cut(s) 226
CseI GACGC 1 cut(s) 907
Csp6I GTAC 3 cut(s) 503, 619, 698
CspCI CAANNNNNGTGG 2 cut(s) 472, 507
CviAII CATG 5 cut(s) 253, 315, 616, 884, 943
CviQI GTAC 3 cut(s) 503, 619, 698
DdeI CTNAG 2 cut(s) 230, 688
DpnI GATC 1 cut(s) 567
DpnII GATC 1 cut(s) 565
EaeI YGGCCR 1 cut(s) 815
Eam1104I CTCTTC 2 cut(s) 40, 170
EarI CTCTTC 2 cut(s) 40, 170
Ecl136II GAGCTC 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 250
EcoICRI GAGCTC 1 cut(s) 250
EcoO109I RGGNCCY 1 cut(s) 226
EcoRII CCWGG 1 cut(s) 910
EcoT38I GRGCYC 1 cut(s) 252
FaeI CATG 5 cut(s) 256, 318, 619, 887, 946
FaqI GGGAC 2 cut(s) 401, 910
FatI CATG 5 cut(s) 252, 314, 615, 883, 942
FauI CCCGC 1 cut(s) 766
Fnu4HI GCNGC 3 cut(s) 216, 643, 759
FokI GGATG 2 cut(s) 127, 281
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 3 cut(s) 216, 643, 759
FspBI CTAG 3 cut(s) 78, 450, 456
GluI GCNGC 3 cut(s) 216, 643, 759
GsuI CTGGAG 1 cut(s) 242
HaeIII GGCC 2 cut(s) 227, 817
HapII CCGG 2 cut(s) 266, 419
HgaI GACGC 1 cut(s) 907
Hin1I GRCGYC 1 cut(s) 899
Hin1II CATG 5 cut(s) 256, 318, 619, 887, 946
HinfI GANTC 1 cut(s) 395
HpaII CCGG 2 cut(s) 266, 419
HphI GGTGA 1 cut(s) 662
Hpy166II GTNNAC 1 cut(s) 589
Hpy188I TCNGA 3 cut(s) 394, 547, 805
Hpy188III TCNNGA 3 cut(s) 221, 662, 764
Hpy8I GTNNAC 1 cut(s) 589
Hpy99I CGWCG 1 cut(s) 134
HpyAV CCTTC 1 cut(s) 287
HpyCH4III ACNGT 2 cut(s) 502, 638
HpyCH4IV ACGT 2 cut(s) 330, 654
HpyCH4V TGCA 7 cut(s) 308, 443, 737, 758, 779, 830, 854
HpyF10VI GCNNNNNNNGC 4 cut(s) 224, 758, 907, 916
HpyF3I CTNAG 2 cut(s) 230, 688
HpySE526I ACGT 2 cut(s) 330, 654
Hsp92I GRCGYC 1 cut(s) 899
Hsp92II CATG 5 cut(s) 256, 318, 619, 887, 946
Kzo9I GATC 1 cut(s) 565
LguI GCTCTTC 1 cut(s) 40
LmnI GCTCC 1 cut(s) 688
Lsp1109I GCAGC 2 cut(s) 654, 770
LweI GCATC 1 cut(s) 724
MaeI CTAG 3 cut(s) 78, 450, 456
MaeII ACGT 2 cut(s) 330, 654
MaeIII GTNAC 2 cut(s) 650, 716
MalI GATC 1 cut(s) 567
MbiI CCGCTC 1 cut(s) 218
MboI GATC 1 cut(s) 565
MboII GAAGA 4 cut(s) 9, 27, 157, 901
MfeI CAATTG 4 cut(s) 792, 843, 1023, 1033
MflI RGATCY 1 cut(s) 565
MhlI GDGCHC 2 cut(s) 100, 252
MlsI TGGCCA 1 cut(s) 817
MluNI TGGCCA 1 cut(s) 817
MlyI GAGTC 1 cut(s) 389
Mox20I TGGCCA 1 cut(s) 817
MroXI GAANNNNTTC 1 cut(s) 276
MscI TGGCCA 1 cut(s) 817
MseI TTAA 2 cut(s) 612, 879
MslI CAYNNNNRTG 1 cut(s) 47
Msp20I TGGCCA 1 cut(s) 817
MspA1I CMGCKG 1 cut(s) 824
MspI CCGG 2 cut(s) 266, 419
MspR9I CCNGG 2 cut(s) 419, 912
MunI CAATTG 4 cut(s) 792, 843, 1023, 1033
Mva1269I GAATGC 1 cut(s) 126
MvaI CCWGG 1 cut(s) 912
MwoI GCNNNNNNNGC 4 cut(s) 224, 758, 907, 916
NciI CCSGG 1 cut(s) 419
NdeII GATC 1 cut(s) 565
NheI GCTAGC 1 cut(s) 77
NlaIII CATG 5 cut(s) 256, 318, 619, 887, 946
NlaIV GGNNCC 1 cut(s) 567
NmeAIII GCCGAG 1 cut(s) 927
NmuCI GTSAC 1 cut(s) 650
NspI RCATGY 2 cut(s) 619, 887
PciI ACATGT 2 cut(s) 615, 883
PciSI GCTCTTC 1 cut(s) 40
PctI GAATGC 1 cut(s) 126
PdmI GAANNNNTTC 1 cut(s) 276
PflMI CCANNNNNTGG 1 cut(s) 824
PkrI GCNGC 3 cut(s) 217, 644, 760
PleI GAGTC 1 cut(s) 389
PpsI GAGTC 1 cut(s) 389
PscI ACATGT 2 cut(s) 615, 883
PshAI GACNNNNGTC 1 cut(s) 657
PsiI TTATAA 1 cut(s) 864
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 1 cut(s) 910
PspGI CCWGG 1 cut(s) 910
PspN4I GGNNCC 1 cut(s) 567
PspPI GGNCC 1 cut(s) 226
PsuI RGATCY 1 cut(s) 565
PvuII CAGCTG 1 cut(s) 824
RsaI GTAC 3 cut(s) 504, 620, 699
RsaNI GTAC 3 cut(s) 503, 619, 698
RseI CAYNNNNRTG 1 cut(s) 47
SacI GAGCTC 1 cut(s) 252
SapI GCTCTTC 1 cut(s) 40
SaqAI TTAA 2 cut(s) 612, 879
SatI GCNGC 3 cut(s) 216, 643, 759
Sau3AI GATC 1 cut(s) 565
Sau96I GGNCC 1 cut(s) 226
ScaI AGTACT 1 cut(s) 504
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 2 cut(s) 419, 912
SduI GDGCHC 2 cut(s) 100, 252
SfaNI GCATC 1 cut(s) 724
SfcI CTRYAG 1 cut(s) 498
SmiMI CAYNNNNRTG 1 cut(s) 47
SmlI CTYRAG 1 cut(s) 764
SmoI CTYRAG 1 cut(s) 764
SsiI CCGC 4 cut(s) 216, 368, 602, 773
SspMI CTAG 3 cut(s) 78, 450, 456
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 2 cut(s) 417, 910
TaaI ACNGT 2 cut(s) 502, 638
TaiI ACGT 2 cut(s) 333, 657
TaqI TCGA 2 cut(s) 47, 732
TatI WGTACW 2 cut(s) 502, 697
TauI GCSGC 1 cut(s) 218
Tru1I TTAA 2 cut(s) 612, 879
Tru9I TTAA 2 cut(s) 612, 879
TscAI CASTG 3 cut(s) 490, 643, 652
TseFI GTSAC 1 cut(s) 650
TseI GCWGC 2 cut(s) 642, 758
Tsp45I GTSAC 1 cut(s) 650
TspDTI ATGAA 2 cut(s) 285, 393
TspRI CASTG 3 cut(s) 490, 643, 652
Van91I CCANNNNNTGG 1 cut(s) 824
XapI RAATTY 3 cut(s) 353, 832, 871
XceI RCATGY 2 cut(s) 619, 887
XmnI GAANNNNTTC 1 cut(s) 276
XspI CTAG 3 cut(s) 78, 450, 456
ZrmI AGTACT 1 cut(s) 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.