pycom12g09310
MYB Family

PHR1-LIKE 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0005519
Physical Location & Seq
Reverse (-)
11203347 .. 11204935
1589 bp
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UTR
Exon/CDS
Intron
pycom12g09310.1

Sequence Viewer

Length: 630 bp
ATGGGTGTTCCAGGACTAACTATTTATCATGTGAAGAGTCATTTACAGAAATACCGACTTGCAAAGTACCTGCCAGAGTCTCCAGCTGATGGTTCTAAGGATGAGAAGAAGGGTTCTGGAGACAGCCTATCTTGCTCAGATTCTTCTCCTGGAGTGCAAATTAATGAGGCATTGAGGATGCAAATGGAAGTTCAGAAGCGTCTTCATGAACAGCTTGAGGTTCAAAGACAGTTGCAAATGAGAATAGAAGCTCAGGGTAAATACTTGCAGAAGATCATCGAGGAGCAGCAAAAGTATGGTGGTTCACTCAAAGCGTCAGAAGCGTTACCATCAGCCGAGGAAAAGCAGAAGCCAGCTCAGTTGGAGACAATGGGCGATGCATCGGCCGCTCCCTCATCTCCCCGAAAGAAACAAAGGGTGGATGAAGGGTTGCCAGACGGTTGCACCACATCTAATCTGCCTCTAAAAGCTGACCAGAAGAACGAATTTGTGGGTCAGTGGGATCGAGACCTGTATGGAAGTGACGGTGGATATGGATTTGGCTTGCAAACAGAGTTTAAAGAAAGAGATGGCGGTGCTGCACAGAAAGCACCTATGGAGTTAGATCCCTTGTGTGGTTCAAAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_CC_LHEQLE Myb_DNA-binding

Protein Analysis

210

Amino Acids

23.02

Weight (kDa)

6.0

Isoelectric Point (pI)

52.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_CC_LHEQLE PF14379 52 - 98 5.2e-25 MYB-CC type transfactor, LHEQLE motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 78
AccB7I CCANNNNNTGG 1 cut(s) 89
AccBSI CCGCTC 1 cut(s) 389
AciI CCGC 2 cut(s) 387, 573
AclWI GGATC 2 cut(s) 510, 599
AcoI YGGCCR 1 cut(s) 384
AcsI RAATTY 1 cut(s) 485
AfaI GTAC 1 cut(s) 68
AfiI CCNNNNNNNGG 2 cut(s) 89, 614
AgsI TTSAA 2 cut(s) 224, 621
AjnI CCWGG 2 cut(s) 10, 148
AluBI AGCT 5 cut(s) 86, 214, 251, 356, 470
AluI AGCT 5 cut(s) 86, 214, 251, 356, 470
Alw26I GTCTC 4 cut(s) 84, 114, 359, 501
AlwI GGATC 2 cut(s) 510, 599
AoxI GGCC 1 cut(s) 384
ApeKI GCWGC 2 cut(s) 286, 578
ApoI RAATTY 1 cut(s) 485
AseI ATTAAT 1 cut(s) 162
BbsI GAAGAC 1 cut(s) 194
BbvI GCAGC 2 cut(s) 298, 565
BccI CCATC 3 cut(s) 83, 337, 563
BciT130I CCWGG 2 cut(s) 12, 150
BcoDI GTCTC 4 cut(s) 84, 114, 359, 501
BfuAI ACCTGC 1 cut(s) 78
BisI GCNGC 3 cut(s) 287, 387, 579
BlsI GCNGC 3 cut(s) 288, 388, 580
Bme1390I CCNGG 2 cut(s) 12, 150
BmrFI CCNGG 2 cut(s) 12, 150
BmsI GCATC 3 cut(s) 168, 367, 389
BpiI GAAGAC 1 cut(s) 194
BpmI CTGGAG 3 cut(s) 66, 138, 171
Bpu10I CCTNAGC 1 cut(s) 252
BpuEI CTTGAG 1 cut(s) 236
BsaI GGTCTC 1 cut(s) 501
BsaJI CCNNGG 1 cut(s) 336
Bsc4I CCNNNNNNNGG 2 cut(s) 89, 614
BseBI CCWGG 2 cut(s) 12, 150
BseDI CCNNGG 1 cut(s) 336
BseGI GGATG 3 cut(s) 106, 183, 427
BseLI CCNNNNNNNGG 2 cut(s) 89, 614
BseMII CTCAG 3 cut(s) 150, 266, 371
BseRI GAGGAG 1 cut(s) 296
BseX3I CGGCCG 1 cut(s) 384
BseXI GCAGC 2 cut(s) 298, 565
BsgI GTGCAG 1 cut(s) 564
Bsh1285I CGRYCG 1 cut(s) 387
BshFI GGCC 1 cut(s) 386
BsiEI CGRYCG 1 cut(s) 387
BslI CCNNNNNNNGG 2 cut(s) 89, 614
BsmAI GTCTC 4 cut(s) 84, 114, 359, 501
BsnI GGCC 1 cut(s) 386
Bso31I GGTCTC 1 cut(s) 501
Bsp143I GATC 3 cut(s) 273, 502, 604
BspACI CCGC 2 cut(s) 387, 573
BspANI GGCC 1 cut(s) 386
BspCNI CTCAG 3 cut(s) 149, 265, 370
BspHI TCATGA 1 cut(s) 205
BspMI ACCTGC 1 cut(s) 78
BspPI GGATC 2 cut(s) 510, 599
BspTNI GGTCTC 1 cut(s) 501
BsrBI CCGCTC 1 cut(s) 389
BssECI CCNNGG 1 cut(s) 336
BssMI GATC 3 cut(s) 273, 502, 604
Bst2UI CCWGG 2 cut(s) 12, 150
Bst4CI ACNGT 3 cut(s) 231, 440, 527
Bst6I CTCTTC 1 cut(s) 29
BstC8I GCNNGC 2 cut(s) 354, 545
BstDEI CTNAG 4 cut(s) 96, 136, 252, 357
BstF5I GGATG 3 cut(s) 106, 183, 427
BstKTI GATC 3 cut(s) 276, 505, 607
BstMAI GTCTC 4 cut(s) 84, 114, 359, 501
BstMBI GATC 3 cut(s) 273, 502, 604
BstMCI CGRYCG 1 cut(s) 387
BstMWI GCNNNNNNNGC 4 cut(s) 132, 320, 386, 587
BstNI CCWGG 2 cut(s) 12, 150
BstSCI CCNGG 2 cut(s) 10, 148
BstV1I GCAGC 2 cut(s) 298, 565
BstV2I GAAGAC 1 cut(s) 194
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
BstZI CGGCCG 1 cut(s) 384
BsuRI GGCC 1 cut(s) 386
BtgZI GCGATG 1 cut(s) 390
BtsCI GGATG 3 cut(s) 106, 183, 427
BtsIMutI CAGTG 1 cut(s) 503
BveI ACCTGC 1 cut(s) 78
Cac8I GCNNGC 2 cut(s) 354, 545
CciI TCATGA 1 cut(s) 205
CseI GACGC 2 cut(s) 188, 303
Csp6I GTAC 1 cut(s) 67
CviAII CATG 2 cut(s) 29, 206
CviQI GTAC 1 cut(s) 67
DdeI CTNAG 4 cut(s) 96, 136, 252, 357
DpnI GATC 3 cut(s) 275, 504, 606
DpnII GATC 3 cut(s) 273, 502, 604
DraI TTTAAA 1 cut(s) 559
EaeI YGGCCR 1 cut(s) 384
EagI CGGCCG 1 cut(s) 384
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
EclXI CGGCCG 1 cut(s) 384
Eco31I GGTCTC 1 cut(s) 501
Eco52I CGGCCG 1 cut(s) 384
EcoRII CCWGG 2 cut(s) 10, 148
EcoT22I ATGCAT 1 cut(s) 382
FaeI CATG 2 cut(s) 32, 209
FaiI YATR 6 cut(s) 30, 207, 297, 516, 534, 596
FatI CATG 2 cut(s) 28, 205
Fnu4HI GCNGC 3 cut(s) 287, 387, 579
FokI GGATG 3 cut(s) 113, 190, 434
Fsp4HI GCNGC 3 cut(s) 287, 387, 579
GluI GCNGC 3 cut(s) 287, 387, 579
GsuI CTGGAG 3 cut(s) 66, 138, 171
HaeIII GGCC 1 cut(s) 386
HgaI GACGC 2 cut(s) 188, 303
Hin1II CATG 2 cut(s) 32, 209
HinfI GANTC 3 cut(s) 37, 77, 140
Hpy166II GTNNAC 1 cut(s) 305
Hpy188I TCNGA 3 cut(s) 139, 195, 319
Hpy188III TCNNGA 3 cut(s) 117, 206, 506
Hpy8I GTNNAC 1 cut(s) 305
HpyAV CCTTC 2 cut(s) 103, 419
HpyCH4III ACNGT 3 cut(s) 231, 440, 527
HpyCH4V TGCA 9 cut(s) 62, 157, 181, 235, 268, 380, 444, 547, 581
HpyF10VI GCNNNNNNNGC 4 cut(s) 132, 320, 386, 587
HpyF3I CTNAG 4 cut(s) 96, 136, 252, 357
Hsp92II CATG 2 cut(s) 32, 209
Kzo9I GATC 3 cut(s) 273, 502, 604
LmnI GCTCC 2 cut(s) 283, 394
Lsp1109I GCAGC 2 cut(s) 298, 565
LweI GCATC 3 cut(s) 168, 367, 389
MaeIII GTNAC 2 cut(s) 324, 521
MalI GATC 3 cut(s) 275, 504, 606
MbiI CCGCTC 1 cut(s) 389
MboI GATC 3 cut(s) 273, 502, 604
MboII GAAGA 6 cut(s) 46, 118, 135, 194, 283, 490
MflI RGATCY 1 cut(s) 604
MluCI AATT 2 cut(s) 159, 485
MlyI GAGTC 2 cut(s) 46, 86
MmeI TCCRAC 1 cut(s) 342
MnlI CCTC 7 cut(s) 160, 168, 211, 274, 331, 403, 471
Mph1103I ATGCAT 1 cut(s) 382
MseI TTAA 2 cut(s) 162, 558
MspA1I CMGCKG 1 cut(s) 86
MspR9I CCNGG 2 cut(s) 12, 150
MvaI CCWGG 2 cut(s) 12, 150
MwoI GCNNNNNNNGC 4 cut(s) 132, 320, 386, 587
NdeII GATC 3 cut(s) 273, 502, 604
NlaIII CATG 2 cut(s) 32, 209
NmeAIII GCCGAG 1 cut(s) 361
NmuCI GTSAC 1 cut(s) 521
NsiI ATGCAT 1 cut(s) 382
PagI TCATGA 1 cut(s) 205
PfeI GAWTC 1 cut(s) 140
PflMI CCANNNNNTGG 1 cut(s) 89
PfoI TCCNGGA 2 cut(s) 10, 148
PkrI GCNGC 3 cut(s) 288, 388, 580
PleI GAGTC 2 cut(s) 45, 85
PpsI GAGTC 2 cut(s) 45, 85
PshBI ATTAAT 1 cut(s) 162
Psp6I CCWGG 2 cut(s) 10, 148
PspGI CCWGG 2 cut(s) 10, 148
PsuI RGATCY 1 cut(s) 604
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 1 cut(s) 68
RsaNI GTAC 1 cut(s) 67
SaqAI TTAA 2 cut(s) 162, 558
SatI GCNGC 3 cut(s) 287, 387, 579
Sau3AI GATC 3 cut(s) 273, 502, 604
SchI GAGTC 2 cut(s) 46, 86
ScrFI CCNGG 2 cut(s) 12, 150
SetI ASST 9 cut(s) 72, 88, 216, 222, 253, 358, 472, 513, 595
SfaNI GCATC 3 cut(s) 168, 367, 389
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 2 cut(s) 159, 485
SsiI CCGC 2 cut(s) 387, 573
StyD4I CCNGG 2 cut(s) 10, 148
TaaI ACNGT 3 cut(s) 231, 440, 527
TaqI TCGA 2 cut(s) 279, 505
TasI AATT 2 cut(s) 159, 485
TauI GCSGC 1 cut(s) 389
TfiI GAWTC 1 cut(s) 140
Tru1I TTAA 2 cut(s) 162, 558
Tru9I TTAA 2 cut(s) 162, 558
TscAI CASTG 1 cut(s) 503
TseFI GTSAC 1 cut(s) 521
TseI GCWGC 2 cut(s) 286, 578
Tsp45I GTSAC 1 cut(s) 521
TspDTI ATGAA 3 cut(s) 194, 222, 438
TspRI CASTG 1 cut(s) 503
Van91I CCANNNNNTGG 1 cut(s) 89
VspI ATTAAT 1 cut(s) 162
XapI RAATTY 1 cut(s) 485
Zsp2I ATGCAT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.