pycom12g10210

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0008872
Physical Location & Seq
Forward (+)
12218816 .. 12223975
5160 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3543 bp
ATGTTCAGGGTGCACAAGAACAGGCCTGCTAAATCAGGGGAGAGGGTGGATTTCAAGTTCTCCCACTTTAAGGCCCTCCAGGTCCCCAGAGGATGGGACAAGCTGTTTGTGTCTATCGTCTCTGTAGAAACTGGGAAACCAATTGCGAAATCAAGCAAAGCAGTGGTGCGCAACGGAAACTGTCAATGGACTGAGACTCTCTGCGAATCTATATGGATTTCCCAAGATGAGTCGTCGAAGGAGATGGAAGATTATTTCATCAAGCTTGTTATTTCCATGGGATCAGCAAGATCCGGTATCCTTGGAGAGGTTTCAGTCAATATGTCGGATTACATTAGTTCAACCTCTTCTGCTCCGGTTTCTCTTCCATTGAAAAAATGCAGTTTCGGGACAATTCTGCAGGTAAAAATTAACTGCCTCACACCAAGAAAAAGGCTCAGTGATGAAGAGTCAAAAGAGACAAGTGCTCATTTGGAAGAAGCAAATGCAAATGGTCATGATGCAGACAGCAAGTCAGATGGATCTTCCTTCGGAAAAGATTTTGGCTTGATCTCAAATCCTGGAGAGCCTGGGAGTCGAGGTAGCAGTTTCTCCACATCAGGTTCACATCGCAGCTATGACTCAGCGGAGGGCTCCATAAGAAGGGATAATATGTCCCCGAGAAGCAACCTTAGTGTTGAAGGCAACCACCTGATAGGAAGACAAGATTCAATCACCTCCCTAATTAGTACAACTCATGGAAACTTTCCTGCTGATATCCCTTCTCCATCAAATAATTCATCATTTAATTCCAGGATTAATGGTTTAGGAAATCATTCTCAGAATTCTCGGAAAGATTTTAAAGAATCATCTTTAAGAGCCACTGATTCTTCTAAAAACCTTCTGGATGCGGCAGAAGTTACGATTGAAGAATTACATGCGGAAGCTAAGATGTGGGAGAGGAACGCCAGGAAGCTAATGCTTGATTTGGATATACTGAGGACAGAATTCTCTGATCGGTCCAAAAAGCAGGCAAATATGAATGTGGAGCTTTCAGCAGCATATGCAGAACGCAATGGCTTGAAGAAAGAAGTTGAACACTTGCATTTGTTGCTTGAGAATTCAGTGGTTAAACAAACAGCCAGTGAGGATTTAACTTATCAGGGCGAAGGAACGCCAGAGATTGAGAAGGCGTTGCAAGATGAGCTGAAGTTTCAAAAAGAATCCGTTGCCAATCTGGCTCTGCAGTTGGAGAGAAGTCAAGAATCAAATATTGAGCTGGTTTCGGTTCTCCAGGAGCTGGAAGAGACAATTGAAAACCAAAAGGTGGAGTTGGAGAATCTTTCAGAACTACAGTCGACATTTGATGATGTGGAAAATTTGATCAAATTAACCAGAGAGGAAAACAGAAATTTAAAGCTTCAGCTGCAACAATTGCAGGAATCAGAGAACAAGTTGCAAGTCACGGTGCAACAGTTTGAACAAACTGTGGAAGAGAAAAACCATGAGATAGAGAATGGATCGAATCTGAACGAACACTTCGTTTTGGACATTGAAACAGAATACAAAAGTAAACTGCTTTTGAAAGAACAAGAAATTGTTAAACTGAAAGCAAAGGTATCCGAGTCTCTCAAAGAAAGGCATTCTACAGAAATGGATTCCATCACTGTGAGTGGGGGTGAAGCAGATCTGATCAGCGAAATTGAGGACTTGAAAGAGAAGGTACAGGAGCTAGAAAGAGATTGTAATGAGCTGACAGAAGAAAACTTAGAGCTCTTGTTCAAGCTTAAGGCAGGGAAGAAAAAATCTTCAGGGGGACACGCACCTGGTGAACTTCCATCCAGTGAGCTTTTATTCGATTCTTTTACCAGCTTTGAGTCCAAAATAACTGAAAATAAACCCCAAATACAAAATGTGGAGGACATGCTTAACAAGAAAGTGCTTGGGGAGATTACAAATAATAATGATGTTTCAGTTCAAGTACTTGACAGCTTGAAAATGGAACTAGAAAGTAAAGTCACAGAGCTGGGTAAAGAATTGACAGAGAAGAGGTTCGAAATAGAAAAACTTGAGACAAACTTGCTAACCAATGAAGAAGAAATCAGTCTTCTAAAGGGGGTCCAGAATGAATTAGAAGCTAAGGTTTCCGATCTTCAGAAGGAAAAAATACAGCTAGAGGAACACATGGAAGTTGTATTAAGGGAAAGCGATATCAGCTCAAAGTGTTTGAATGATTTGCGGCATGAATTAATGGTGCTTAGCAGAAGTGTGAATTCCCATGTTTCCACACATAGGGTACTTGAAAGGAAATCTTCGGAGCTAGAAGCTGATAAATGTGAACTGGACCGTCACATATCAGAGCTTGAACAAGAAAACATACAGTTGTCAGCAAGTGTTTCTGCACTCGAAGCTCAAATAAGATGTTTGACAGATGAAAAAGAGGCCAGTCAATTAGAATTAGAGAATTCCCAATCTTATTCTCTGAGTCTCCAAGATGAGATCATTAGATTGAAAACTGAGATGGAGTCTGATAAAGTTGAACTGAAACAGAAATTAAGGGACTTGGAGAGTCAGTGGTCAGAAGCTCGAGATGAATGTGAATTTCTAAAAAGGGCAAACCCGGAGCTACAAGCTACTGCCGAGAGCCTCATTGAAGAGTGCAATTCTCTTCAGAATTCAAATGACGAGTTGAGGAAACAAAAGCTGGAGTTGCATCAGCTCTGTTCCGTCTTGGAGGCAAAGTTGAATCAATCGCACAAGAGCTTTACAAATTGCTCCAAAAGAGTTGAAGTACTGGAACAGGATCTTTCTTTAATGCTGGAAAACATCGCCTCCAAAGAGGAGAGCCTGAATTTAGAATTAGATGCACTTCTTGATGAAAACATGAAATATAAGGAGAAGCTTACATTGGAAGAAAGTTTGTTCAACAAGATGTATTTGGAGAAGACGGTTGAAGTTGAGAGCCTTCAGGAAGAGGTAGACCGACTTACCAAGCTACTTGCTGCAACTCAAGAAGAAAGAGAAGAACTAGCTTCAGATGCCGTCCAGGAAGCATCCAGATTACGAACTGAGAAGGAAATGCTTGAATCTGCCCTTCGAGAGGTTCAATCTAAAGCAATACAGACTGAGGAAGAATTGAATATTATGAGAATAGAAACTGAAGCGAAGCTACAAGGCCTGGTCGCTGAGCTTGCTGCCTCTAAACAAAACCAGGAAGTAACGATGGCTGACCATGAAAGGCTATTGAAGTTGTTGGAGAATTACAAATCGAGTGAAGCAAAACTGAAGACCACTGTCAATGACCTTGAACTAAGGCTTACAGTTTCTGATTATGAACGTCAACAAGTGTTGGAAGAATCTATTAATATGAAGGTTCAGATTGAGAAACTAACTCATTGTCAGGAGGAAGTTTTGGCTTTCAACAATGAGCTGGATGCAACCAAGTTTGAGAAAGAAAAATTGGAAGTGTTACTGCATTCAATTTCTGAGGAATGTCGAGATCTGAAGGCAGAGAAAAGTTCATTCCATGAGATAGATCTCAACATTGGAGAAGGCCTTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1181

Amino Acids

133.92

Weight (kDa)

4.86

Isoelectric Point (pI)

52.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 24 - 140 7.8e-13 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 170
Acc36I ACCTGC 1 cut(s) 391
AccB7I CCANNNNNTGG 3 cut(s) 93, 1279, 1306
AccI GTMKAC 2 cut(s) 1337, 2958
AciI CCGC 4 cut(s) 626, 890, 920, 2218
AclWI GGATC 5 cut(s) 285, 289, 529, 1507, 2790
AdeI CACNNNGTG 1 cut(s) 1808
AfaI GTAC 5 cut(s) 730, 1704, 1962, 2277, 2772
AfiI CCNNNNNNNGG 8 cut(s) 70, 93, 307, 642, 1279, 1306, 2271, 3079
AflII CTTAAG 1 cut(s) 1766
AhdI GACNNNNNGTC 1 cut(s) 511
AjuI GAANNNNNNNTTGG 4 cut(s) 2870, 2902, 3422, 3454
AloI GAACNNNNNNTCC 2 cut(s) 41, 73
Alw21I GWGCWC 3 cut(s) 15, 469, 1755
Alw26I GTCTC 7 cut(s) 124, 188, 452, 1280, 1611, 2045, 2471
Alw44I GTGCAC 1 cut(s) 11
AlwI GGATC 5 cut(s) 285, 289, 529, 1507, 2790
AlwNI CAGNNNCTG 2 cut(s) 1279, 3305
Ama87I CYCGRG 2 cut(s) 658, 2565
AoxI GGCC 5 cut(s) 23, 72, 2421, 3154, 3532
ApaLI GTGCAC 1 cut(s) 11
ApeKI GCWGC 5 cut(s) 612, 1037, 1405, 2981, 3173
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
AseI ATTAAT 3 cut(s) 798, 2228, 3342
Asp700I GAANNNNTTC 2 cut(s) 814, 2030
AspLEI GCGC 1 cut(s) 171
AspS9I GGNCC 5 cut(s) 73, 82, 999, 2098, 2323
AsuC2I CCSGG 1 cut(s) 2600
AsuHPI GGTGA 3 cut(s) 706, 1670, 1820
AsuII TTCGAA 1 cut(s) 2034
AvaI CYCGRG 2 cut(s) 658, 2565
AvaII GGWCC 4 cut(s) 82, 999, 2098, 2323
BaeGI GKGCMC 1 cut(s) 15
BanII GRGCYC 2 cut(s) 635, 1755
BarI GAAGNNNNNNTAC 2 cut(s) 3132, 3164
BbsI GAAGAC 4 cut(s) 706, 2078, 2930, 3272
Bbv12I GWGCWC 3 cut(s) 15, 469, 1755
BbvI GCAGC 5 cut(s) 624, 1049, 1392, 2968, 3160
BccI CCATC 8 cut(s) 87, 238, 512, 775, 1649, 1825, 2494, 3196
BceAI ACGGC 1 cut(s) 3005
BciVI GTATCC 2 cut(s) 308, 1609
BclI TGATCA 2 cut(s) 1362, 1671
BcnI CCSGG 1 cut(s) 2600
BcoDI GTCTC 7 cut(s) 124, 188, 452, 1280, 1611, 2045, 2471
BfaI CTAG 5 cut(s) 1712, 1985, 2153, 2300, 3008
BfmI CTRYAG 5 cut(s) 123, 398, 1223, 1331, 1626
BfrI CTTAAG 1 cut(s) 1766
BfuAI ACCTGC 1 cut(s) 391
BfuI GTATCC 2 cut(s) 308, 1609
BglI GCCNNNNNGGC 1 cut(s) 1217
BglII AGATCT 3 cut(s) 1666, 3478, 3514
BisI GCNGC 7 cut(s) 613, 891, 1038, 1406, 2219, 2982, 3174
BlpI GCTNAGC 2 cut(s) 2237, 3165
BlsI GCNGC 7 cut(s) 614, 892, 1039, 1407, 2220, 2983, 3175
BmcAI AGTACT 2 cut(s) 1962, 2772
Bme18I GGWCC 4 cut(s) 82, 999, 2098, 2323
BmeRI GACNNNNNGTC 1 cut(s) 511
BmeT110I CYCGRG 2 cut(s) 658, 2565
BmgT120I GGNCC 5 cut(s) 73, 82, 999, 2098, 2323
BmiI GGNNCC 3 cut(s) 84, 634, 2099
BmrI ACTGGG 1 cut(s) 141
BmsI GCATC 7 cut(s) 490, 877, 2701, 2833, 3007, 3041, 3403
BmuI ACTGGG 1 cut(s) 141
BoxI GACNNNNGTC 1 cut(s) 3272
BpiI GAAGAC 4 cut(s) 706, 2078, 2930, 3272
BpmI CTGGAG 4 cut(s) 62, 582, 1256, 2705
Bpu10I CCTNAGC 1 cut(s) 2118
Bpu1102I GCTNAGC 2 cut(s) 2237, 3165
Bpu14I TTCGAA 1 cut(s) 2034
BpuEI CTTGAG 3 cut(s) 1115, 2069, 2973
BpuMI CCSGG 1 cut(s) 2600
BsaJI CCNNGG 3 cut(s) 276, 301, 569
BsaWI WCCGGW 2 cut(s) 293, 355
BsaXI ACNNNNNCTCC 8 cut(s) 565, 595, 2537, 2567, 2705, 2735, 2795, 2825
Bsc4I CCNNNNNNNGG 8 cut(s) 70, 93, 307, 642, 1279, 1306, 2271, 3079
Bse1I ACTGG 6 cut(s) 136, 1122, 1821, 2325, 2424, 2778
Bse3DI GCAATG 1 cut(s) 1060
BseDI CCNNGG 3 cut(s) 276, 301, 569
BseGI GGATG 5 cut(s) 98, 892, 1817, 3032, 3418
BseLI CCNNNNNNNGG 8 cut(s) 70, 93, 307, 642, 1279, 1306, 2271, 3079
BseMI GCAATG 1 cut(s) 1060
BseNI ACTGG 6 cut(s) 136, 1122, 1821, 2325, 2424, 2778
BseRI GAGGAG 1 cut(s) 2834
BseSI GKGCMC 1 cut(s) 15
BseXI GCAGC 5 cut(s) 624, 1049, 1392, 2968, 3160
BseYI CCCAGC 1 cut(s) 2005
BsgI GTGCAG 1 cut(s) 2364
BshFI GGCC 5 cut(s) 25, 74, 2423, 3156, 3534
BsiHKAI GWGCWC 3 cut(s) 15, 469, 1755
BsiHKCI CYCGRG 2 cut(s) 658, 2565
BsiSI CCGG 3 cut(s) 294, 356, 2600
BslFI GGGAC 6 cut(s) 68, 110, 403, 640, 1809, 2552
BslI CCNNNNNNNGG 8 cut(s) 70, 93, 307, 642, 1279, 1306, 2271, 3079
BsmAI GTCTC 7 cut(s) 124, 188, 452, 1280, 1611, 2045, 2471
BsmBI CGTCTC 1 cut(s) 124
BsmFI GGGAC 6 cut(s) 68, 110, 403, 640, 1809, 2552
BsmI GAATGC 2 cut(s) 1621, 3454
BsnI GGCC 5 cut(s) 25, 74, 2423, 3156, 3534
BsoBI CYCGRG 2 cut(s) 658, 2565
Bsp119I TTCGAA 1 cut(s) 2034
Bsp1286I GDGCHC 4 cut(s) 15, 469, 635, 1755
Bsp1720I GCTNAGC 2 cut(s) 2237, 3165
Bsp19I CCATGG 1 cut(s) 276
BspACI CCGC 4 cut(s) 626, 890, 920, 2218
BspANI GGCC 5 cut(s) 25, 74, 2423, 3156, 3534
BspHI TCATGA 1 cut(s) 496
BspLI GGNNCC 3 cut(s) 84, 634, 2099
BspMAI CTGCAG 2 cut(s) 402, 1227
BspMI ACCTGC 1 cut(s) 391
BspPI GGATC 5 cut(s) 285, 289, 529, 1507, 2790
BspT104I TTCGAA 1 cut(s) 2034
BspTI CTTAAG 1 cut(s) 1766
BsrDI GCAATG 1 cut(s) 1060
BsrI ACTGG 6 cut(s) 136, 1122, 1821, 2325, 2424, 2778
BssECI CCNNGG 3 cut(s) 276, 301, 569
BssT1I CCWWGG 2 cut(s) 276, 301
Bst6I CTCTTC 9 cut(s) 352, 369, 441, 1278, 1467, 2021, 2628, 2652, 2946
BstAFI CTTAAG 1 cut(s) 1766
BstAPI GCANNNNNTGC 3 cut(s) 1043, 1090, 1414
BstBI TTCGAA 1 cut(s) 2034
BstC8I GCNNGC 3 cut(s) 27, 1011, 3171
BstDSI CCRYGG 1 cut(s) 276
BstENI CCTNNNNNAGG 2 cut(s) 305, 3077
BstF5I GGATG 5 cut(s) 98, 892, 1817, 3032, 3418
BstHHI GCGC 1 cut(s) 171
BstMAI GTCTC 7 cut(s) 124, 188, 452, 1280, 1611, 2045, 2471
BstNSI RCATGY 2 cut(s) 920, 1906
BstPAI GACNNNNGTC 1 cut(s) 3272
BstSFI CTRYAG 5 cut(s) 123, 398, 1223, 1331, 1626
BstSLI GKGCMC 1 cut(s) 15
BstV1I GCAGC 5 cut(s) 624, 1049, 1392, 2968, 3160
BstV2I GAAGAC 4 cut(s) 706, 2078, 2930, 3272
BstX2I RGATCY 6 cut(s) 290, 521, 1666, 2782, 3478, 3514
BstYI RGATCY 6 cut(s) 290, 521, 1666, 2782, 3478, 3514
BsuI GTATCC 2 cut(s) 308, 1609
BsuRI GGCC 5 cut(s) 25, 74, 2423, 3156, 3534
BtgI CCRYGG 1 cut(s) 276
BtgZI GCGATG 2 cut(s) 593, 2791
BtsCI GGATG 5 cut(s) 98, 892, 1817, 3032, 3418
BtsI GCAGTG 1 cut(s) 168
BtsIMutI CAGTG 9 cut(s) 168, 445, 861, 1110, 1129, 1644, 1828, 2558, 3270
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 3 cut(s) 27, 1011, 3171
CaiI CAGNNNCTG 2 cut(s) 1279, 3305
CciI TCATGA 1 cut(s) 496
CfoI GCGC 1 cut(s) 171
Cfr13I GGNCC 5 cut(s) 73, 82, 999, 2098, 2323
CsiI ACCWGGT 1 cut(s) 1804
Csp6I GTAC 5 cut(s) 729, 1703, 1961, 2276, 2771
CviQI GTAC 5 cut(s) 729, 1703, 1961, 2276, 2771
DraI TTTAAA 2 cut(s) 841, 1395
DraIII CACNNNGTG 1 cut(s) 1808
DriI GACNNNNNGTC 1 cut(s) 511
Eam1104I CTCTTC 9 cut(s) 352, 369, 441, 1278, 1467, 2021, 2628, 2652, 2946
Eam1105I GACNNNNNGTC 1 cut(s) 511
EarI CTCTTC 9 cut(s) 352, 369, 441, 1278, 1467, 2021, 2628, 2652, 2946
Ecl136II GAGCTC 1 cut(s) 1753
Eco130I CCWWGG 2 cut(s) 276, 301
Eco147I AGGCCT 3 cut(s) 25, 3156, 3534
Eco24I GRGCYC 2 cut(s) 635, 1755
Eco32I GATATC 2 cut(s) 757, 2191
Eco47I GGWCC 4 cut(s) 82, 999, 2098, 2323
Eco53kI GAGCTC 1 cut(s) 1753
Eco88I CYCGRG 2 cut(s) 658, 2565
EcoICRI GAGCTC 1 cut(s) 1753
EcoNI CCTNNNNNAGG 2 cut(s) 305, 3077
EcoO109I RGGNCCY 2 cut(s) 73, 82
EcoRI GAATTC 6 cut(s) 823, 986, 1099, 2251, 2443, 2653
EcoRV GATATC 2 cut(s) 757, 2191
EcoT14I CCWWGG 2 cut(s) 276, 301
EcoT38I GRGCYC 2 cut(s) 635, 1755
ErhI CCWWGG 2 cut(s) 276, 301
Esp3I CGTCTC 1 cut(s) 124
FalI AAGNNNNNCTT 2 cut(s) 2275, 2307
FaqI GGGAC 6 cut(s) 68, 110, 403, 640, 1809, 2552
FauNDI CATATG 1 cut(s) 1042
FbaI TGATCA 2 cut(s) 1362, 1671
FblI GTMKAC 2 cut(s) 1337, 2958
Fnu4HI GCNGC 7 cut(s) 613, 891, 1038, 1406, 2219, 2982, 3174
FokI GGATG 5 cut(s) 105, 899, 1804, 3019, 3425
FriOI GRGCYC 2 cut(s) 635, 1755
Fsp4HI GCNGC 7 cut(s) 613, 891, 1038, 1406, 2219, 2982, 3174
FspBI CTAG 5 cut(s) 1712, 1985, 2153, 2300, 3008
FspI TGCGCA 1 cut(s) 170
GlaI GCGC 1 cut(s) 170
GluI GCNGC 7 cut(s) 613, 891, 1038, 1406, 2219, 2982, 3174
GsaI CCCAGC 1 cut(s) 2009
GsuI CTGGAG 4 cut(s) 62, 582, 1256, 2705
HaeIII GGCC 5 cut(s) 25, 74, 2423, 3156, 3534
HapII CCGG 3 cut(s) 294, 356, 2600
HhaI GCGC 1 cut(s) 171
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HincII GTYRAC 2 cut(s) 1338, 3320
HindII GTYRAC 2 cut(s) 1338, 3320
HindIII AAGCTT 4 cut(s) 263, 1397, 1763, 2879
HpaII CCGG 3 cut(s) 294, 356, 2600
HphI GGTGA 3 cut(s) 706, 1670, 1820
Hpy166II GTNNAC 8 cut(s) 13, 605, 1338, 1553, 1811, 2318, 2959, 3320
Hpy8I GTNNAC 8 cut(s) 13, 605, 1338, 1553, 1811, 2318, 2959, 3320
Hpy99I CGWCG 1 cut(s) 238
HpyCH4IV ACGT 1 cut(s) 3316
HpySE526I ACGT 1 cut(s) 3316
HspAI GCGC 1 cut(s) 169
Ksp22I TGATCA 2 cut(s) 1362, 1671
LmnI GCTCC 8 cut(s) 358, 638, 1027, 1276, 1708, 2296, 2602, 2759
Lsp1109I GCAGC 5 cut(s) 624, 1049, 1392, 2968, 3160
LweI GCATC 7 cut(s) 490, 877, 2701, 2833, 3007, 3041, 3403
MabI ACCWGGT 1 cut(s) 1804
MaeI CTAG 5 cut(s) 1712, 1985, 2153, 2300, 3008
MaeII ACGT 1 cut(s) 3316
MaeIII GTNAC 6 cut(s) 898, 1441, 1996, 2327, 3196, 3447
MfeI CAATTG 3 cut(s) 141, 1290, 1412
MflI RGATCY 6 cut(s) 290, 521, 1666, 2782, 3478, 3514
MhlI GDGCHC 4 cut(s) 15, 469, 635, 1755
MmeI TCCRAC 5 cut(s) 306, 1209, 1293, 3213, 3309
MroXI GAANNNNTTC 2 cut(s) 814, 2030
MslI CAYNNNNRTG 1 cut(s) 1646
MspA1I CMGCKG 2 cut(s) 626, 1405
MspCI CTTAAG 1 cut(s) 1766
MspI CCGG 3 cut(s) 294, 356, 2600
MunI CAATTG 3 cut(s) 141, 1290, 1412
Mva1269I GAATGC 2 cut(s) 1621, 3454
NciI CCSGG 1 cut(s) 2600
NcoI CCATGG 1 cut(s) 276
NdeI CATATG 1 cut(s) 1042
NlaIV GGNNCC 3 cut(s) 84, 634, 2099
NmeAIII GCCGAG 1 cut(s) 2644
NmuCI GTSAC 3 cut(s) 1441, 1996, 2327
NsbI TGCGCA 1 cut(s) 170
NspI RCATGY 2 cut(s) 920, 1906
NspV TTCGAA 1 cut(s) 2034
PaeR7I CTCGAG 1 cut(s) 2565
PagI TCATGA 1 cut(s) 496
PceI AGGCCT 3 cut(s) 25, 3156, 3534
PcsI WCGNNNNNNNCGW 1 cut(s) 1518
PctI GAATGC 2 cut(s) 1621, 3454
PdmI GAANNNNTTC 2 cut(s) 814, 2030
PflMI CCANNNNNTGG 3 cut(s) 93, 1279, 1306
PfoI TCCNGGA 4 cut(s) 559, 791, 1272, 3024
PkrI GCNGC 7 cut(s) 614, 892, 1039, 1407, 2220, 2983, 3175
PpuMI RGGWCCY 1 cut(s) 82
PshAI GACNNNNGTC 1 cut(s) 3272
PshBI ATTAAT 3 cut(s) 798, 2228, 3342
Psp124BI GAGCTC 1 cut(s) 1755
Psp5II RGGWCCY 1 cut(s) 82
PspFI CCCAGC 1 cut(s) 2005
PspN4I GGNNCC 3 cut(s) 84, 634, 2099
PspPI GGNCC 5 cut(s) 73, 82, 999, 2098, 2323
PspPPI RGGWCCY 1 cut(s) 82
PstI CTGCAG 2 cut(s) 402, 1227
PstNI CAGNNNCTG 2 cut(s) 1279, 3305
PsuI RGATCY 6 cut(s) 290, 521, 1666, 2782, 3478, 3514
PvuII CAGCTG 1 cut(s) 1405
RsaI GTAC 5 cut(s) 730, 1704, 1962, 2277, 2772
RsaNI GTAC 5 cut(s) 729, 1703, 1961, 2276, 2771
RseI CAYNNNNRTG 1 cut(s) 1646
SacI GAGCTC 1 cut(s) 1755
SalI GTCGAC 1 cut(s) 1336
SatI GCNGC 7 cut(s) 613, 891, 1038, 1406, 2219, 2982, 3174
Sau96I GGNCC 5 cut(s) 73, 82, 999, 2098, 2323
ScaI AGTACT 2 cut(s) 1962, 2772
SduI GDGCHC 4 cut(s) 15, 469, 635, 1755
SexAI ACCWGGT 1 cut(s) 1804
SfaNI GCATC 7 cut(s) 490, 877, 2701, 2833, 3007, 3041, 3403
SfcI CTRYAG 5 cut(s) 123, 398, 1223, 1331, 1626
Sfr274I CTCGAG 1 cut(s) 2565
SfuI TTCGAA 1 cut(s) 2034
SinI GGWCC 4 cut(s) 82, 999, 2098, 2323
SlaI CTCGAG 1 cut(s) 2565
SmiMI CAYNNNNRTG 1 cut(s) 1646
SmlI CTYRAG 5 cut(s) 1094, 1766, 2048, 2565, 2988
SmoI CTYRAG 5 cut(s) 1094, 1766, 2048, 2565, 2988
SseBI AGGCCT 3 cut(s) 25, 3156, 3534
SsiI CCGC 4 cut(s) 626, 890, 920, 2218
SspI AATATT 2 cut(s) 1252, 3121
SspMI CTAG 5 cut(s) 1712, 1985, 2153, 2300, 3008
SstI GAGCTC 1 cut(s) 1755
StuI AGGCCT 3 cut(s) 25, 3156, 3534
StyI CCWWGG 2 cut(s) 276, 301
TaiI ACGT 1 cut(s) 3319
TaqII GACCGA 2 cut(s) 987, 2976
TatI WGTACW 3 cut(s) 728, 1960, 2770
TauI GCSGC 2 cut(s) 893, 2221
TscAI CASTG 9 cut(s) 168, 445, 868, 1110, 1129, 1651, 1828, 2558, 3277
TseFI GTSAC 3 cut(s) 1441, 1996, 2327
TseI GCWGC 5 cut(s) 612, 1037, 1405, 2981, 3173
Tsp45I GTSAC 3 cut(s) 1441, 1996, 2327
TspGWI ACGGA 3 cut(s) 189, 1195, 2695
TspRI CASTG 9 cut(s) 168, 445, 868, 1110, 1129, 1651, 1828, 2558, 3277
Van91I CCANNNNNTGG 3 cut(s) 93, 1279, 1306
Vha464I CTTAAG 1 cut(s) 1766
VneI GTGCAC 1 cut(s) 11
VpaK11BI GGWCC 4 cut(s) 82, 999, 2098, 2323
VspI ATTAAT 3 cut(s) 798, 2228, 3342
XagI CCTNNNNNAGG 2 cut(s) 305, 3077
XceI RCATGY 2 cut(s) 920, 1906
XhoI CTCGAG 1 cut(s) 2565
XmiI GTMKAC 2 cut(s) 1337, 2958
XmnI GAANNNNTTC 2 cut(s) 814, 2030
XspI CTAG 5 cut(s) 1712, 1985, 2153, 2300, 3008
ZrmI AGTACT 2 cut(s) 1962, 2772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.