Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0004290

Physical Location & Seq

Genomic Coordinates Reverse (-)

20026033 .. 20031108

Gene Structure 5076 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1971 bp

ATGAAGGAAAAGCTTGGATTTTTAGTTTGTGTGTGGTTTCTGCTATGTGGGGTTTGTGTGGCGAGGTTCGTGGTGGAGAAGAGCATCTTGAAAGTGACTTCTCCGAGCTCGATGGAGGGTGTGTACGAATGTGCGATTGGGAACTTTGGGGTTCCTCAATACGGAGGAACTTTGGTGGGAACTGTGTACCATCCGAAGACCAATCAGATGGCATGCAAAGGGTTTGAAGATACCGACGAATCCTTCAAACCGAAGCCCGGTGGTCTGCCGAAGTTTGTTCTTGTTGATCGAGGAGATTGTTACTTCACATTGAAGGCATGGAATGCACAGAGAGGGGGAGCGGCCGCCATTCTTGTTATAGATAACAAGAATGAGCAGTTGATTACCATGGACACTCCTGAACAAGAAAATGCGGATGCTGATTATCTGCAGAATATCACAATTCCTTCGGCTCTCATTAGCAAAGAGTTGGGTGACAGTATAAAAAAATCTCTATCGGGTGATGAGATGGTCAACATAAAACTAGACTGGACGGAGGCTCTTCCACATCCTGATGAACGTGTTGAGTATGAGTTTTGGACGAATAGCAATGATGAGTGTGGGCCAAAGTGCGACAGCCAGATTGAGTTTGTGAAGAACTTTAAAGGAGCAGCTCAGGTACTTGAGCGGAAGGGGTACACCCAGTTTACTCCACACTATATAACATGGTACTGTCCAGAAGCTTTTGTTTCGAGCAAACAGTGCAAGTCTCAGTGTATCAATCATGGGAGGTACTGTGCTCCTGATCCAGAGCAAGATTTCAGTAAAGGTTATGATGGGAAAGATGTTGTGGTCCAAAATCTCCGTCAAGCTTGCTTCTATAAAGTGGCCAATGAAAGTGGAAAGCCATGGCTTTGGTGGGACTATGTCACGGACTTTGCAATACGTTGCCCAATGAAAGAGAATAAGTACAACCAAGAATGTGCAAATCAAGTTATTGAATCCCTTGGTGCTGATCTGAAGAAGATACAGAAATGTATTGGCGACCCTGAAGCAGATGAGGAGAATGCAGTTCTGAAGGCCGAACAGGATGCCCAGATTGGCAAGGGCTACCGTGGAGATGTTACAATATTGCCAACTCTTGTAATAAACCGCAGACAGTACAGAGGGAAACTGGACAAAGGAGCTGTTCTCAAAGCCATCTGTTCTGGTTTCCAAGAGACCACTGAGCCCGCTGTTTGTTTAAGTGAAGATGTACAAACAAATCAGTGTTTGGAAAACAATGGAGGTTGCTGGCATGACAAAGCTGCTAACCTTACCGCTTGCAGGGATACGTTCCGTGGACGAGTGTGTGAATGCCCTATTGTGCAAGGCGTAAAGTTTGTTGGCGATGGGTATACTCACTGTGAAGCTACAGGAGCATTACGTTGTGGAATTAACAATGGAGGTTGTTGGAAGGAAACCCAACTCGGCAGGACTTACTCTGCTTGTCGTGATGACCATACAAACGGTTGCAGTTGTCCACCAGGATTCAAGGGTGATGGAGAGAAAACATGTGAAGATGTGGATGAGTGCAAGGAGAAAACGGCTTGCCGATGTGCACAATGCAAATGCAAGAATACTTGGGGCAGTTACGATTGCAGTTGCGGTGGTGGCTTACTGTACATGCAAGGATATGATGCATGCATAAGTAAAAACGCCAGTGGTTCTGGGTCTAGCTGGGGATTAATTTGGATTATCATTCTCTGCTTGGGTGCTGTCGGAGTTGGGGGATATGCAGTTTACAAGTATCGAATCCGGAGATACATGGATTCAGAGATCCGGGCAATCATGGCGCAGTACATGCCGTTGGGTAATGAAGGAGAAGTTCCCGGCCATGTCTCCCGCGGGGATCGATCTGAAACTGGCGGAGACATATTCATAAGATGTAAGATGCTGTCCGAGAGAAAAGGGAAGACAAACGACGTCCTGAAATCCTATGTCCTCCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0005048 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005783 GO:0005794 GO:0005802 GO:0005886 GO:0006605 GO:0006623 GO:0006810 GO:0006886 GO:0006892 GO:0006896 GO:0007034 GO:0008104 GO:0008150 GO:0009940 GO:0010008 GO:0010209 GO:0012505 GO:0012506 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030135 GO:0030136 GO:0030659 GO:0030662 GO:0030665 GO:0031090 GO:0031410 GO:0031902 GO:0031982 GO:0031984 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0071944 GO:0072594 GO:0072665 GO:0072666 GO:0097708 GO:0098588 GO:0098791 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA cEGF

Protein Analysis

657

Amino Acids

72.7

Weight (kDa)

5.74

Isoelectric Point (pI)

34.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PA	PF02225	68 - 161	2.5e-15	PA domain
VSR_TRX	PF25011	188 - 395	1.4e-105	Vacuolar sorting receptor thioredoxin-like domain
cEGF	PF12662	498 - 517	4.2e-07	Complement Clr-like EGF-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004290)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30290 AT2G30290
fragaria_vesca	FvH4_6g09810 FvH4_6g09810 FvH4_6g09810
malus_domestica	MD04G1175100.v1.1 MD12G1188800.v1.1
prunus_persica	Prupe.6G294400_v2.0.a1 Prupe.6G294400_v2.0.a1
pyrus_communis	pycom04g15450 pycom12g17630
rosa_chinensis	RchiOBHm_Chr3g0460421 RchiOBHm_Chr6g0252231
rosa_laevigata	RLG00000025013
rosa_multiflora	Rmu_co8220956.1_g000001 Rmu_co8306627.1_g000001 Rmu_sc0004267.1_g000012 Rmu_sc0006252.1_g000025 Rmu_sc0009799.1_g000013 Rmu_sc0021028.1_g000001 Rmu_sc0025932.1_g000001
rosa_roxburghii	Rroxscaffold_6G00419500 Rroxscaffold_7G00216220
rosa_rugosa	Rorug03G0042500
rosa_samantha	Rh3AG100500 Rh3BG104500 Rh3CG106300 Rh3DG105800 Rh6AG020100 Rh6BG015600
rosa_wichuraiana	Rw3G008550 Rw6G001650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1947
AccBSI	CCGCTC	2 cut(s)	341, 667
AccI	GTMKAC	1 cut(s)	1376
AccII	CGCG	1 cut(s)	1866
AccIII	TCCGGA	1 cut(s)	1776
AclWI	GGATC	3 cut(s)	779, 1792, 1878
AcoI	YGGCCR	3 cut(s)	342, 867, 1852
AcuI	CTGAAG	3 cut(s)	1019, 1050, 1076
AcyI	GRCGYC	1 cut(s)	1944
AfiI	CCNNNNNNNGG	3 cut(s)	161, 257, 1305
AflIII	ACRYGT	2 cut(s)	559, 1532
AgsI	TTSAA	6 cut(s)	91, 227, 247, 313, 980, 1513
AjnI	CCWGG	1 cut(s)	1504
AjuI	GAANNNNNNNTTGG	2 cut(s)	120, 152
AluBI	AGCT	9 cut(s)	13, 108, 653, 722, 851, 1166, 1286, 1391, 1698
AluI	AGCT	9 cut(s)	13, 108, 653, 722, 851, 1166, 1286, 1391, 1698
Alw21I	GWGCWC	3 cut(s)	110, 781, 1582
Alw26I	GTCTC	4 cut(s)	753, 1193, 1864, 1884
Alw44I	GTGCAC	1 cut(s)	1578
AlwI	GGATC	3 cut(s)	779, 1792, 1878
Aor13HI	TCCGGA	1 cut(s)	1776
AoxI	GGCC	5 cut(s)	342, 602, 867, 1059, 1852
ApaLI	GTGCAC	1 cut(s)	1578
ApeKI	GCWGC	2 cut(s)	650, 1286
ArsI	GACNNNNNNTTYG	2 cut(s)	829, 861
AseI	ATTAAT	1 cut(s)	1706
AspLEI	GCGC	1 cut(s)	1816
AspS9I	GGNCC	2 cut(s)	602, 832
AsuC2I	CCSGG	3 cut(s)	258, 1802, 1851
AsuHPI	GGTGA	3 cut(s)	485, 512, 1529
AvaII	GGWCC	1 cut(s)	832
BaeGI	GKGCMC	1 cut(s)	1582
BalI	TGGCCA	1 cut(s)	869
BanII	GRGCYC	2 cut(s)	110, 1212
BbsI	GAAGAC	2 cut(s)	203, 1940
Bbv12I	GWGCWC	3 cut(s)	110, 781, 1582
BbvI	GCAGC	2 cut(s)	662, 1273
BccI	CCATC	8 cut(s)	106, 198, 202, 502, 809, 1187, 1364, 1514
BceAI	ACGGC	2 cut(s)	1581, 1810
BcgI	CGANNNNNNTGC	2 cut(s)	1052, 1086
BciT130I	CCWGG	1 cut(s)	1506
BciVI	GTATCC	1 cut(s)	1303
BcnI	CCSGG	3 cut(s)	258, 1802, 1851
BcoDI	GTCTC	4 cut(s)	753, 1193, 1864, 1884
BfaI	CTAG	2 cut(s)	524, 1695
BfmI	CTRYAG	2 cut(s)	428, 1392
BfuI	GTATCC	1 cut(s)	1303
BisI	GCNGC	4 cut(s)	342, 345, 651, 1287
BlsI	GCNGC	4 cut(s)	343, 346, 652, 1288
Bme1390I	CCNGG	4 cut(s)	258, 1506, 1802, 1851
Bme18I	GGWCC	1 cut(s)	832
BmgT120I	GGNCC	2 cut(s)	602, 832
BmiI	GGNNCC	1 cut(s)	153
BmrFI	CCNGG	4 cut(s)	258, 1506, 1802, 1851
BmrI	ACTGGG	1 cut(s)	676
BmsI	GCATC	5 cut(s)	93, 406, 1060, 1648, 1902
BmuI	ACTGGG	1 cut(s)	676
BpiI	GAAGAC	2 cut(s)	203, 1940
BplI	GAGNNNNNCTC	2 cut(s)	1155, 1187
Bpu10I	CCTNAGC	1 cut(s)	654
BpuEI	CTTGAG	1 cut(s)	683
BpuMI	CCSGG	3 cut(s)	258, 1802, 1851
Bsa29I	ATCGAT	1 cut(s)	1873
BsaHI	GRCGYC	1 cut(s)	1944
BsaI	GGTCTC	1 cut(s)	1193
BsaJI	CCNNGG	6 cut(s)	387, 887, 985, 1093, 1318, 1864
BsaWI	WCCGGW	1 cut(s)	1776
BsaXI	ACNNNNNCTCC	4 cut(s)	107, 137, 760, 790
Bsc4I	CCNNNNNNNGG	3 cut(s)	161, 257, 1305
Bse1I	ACTGG	5 cut(s)	533, 682, 1158, 1680, 1888
Bse3DI	GCAATG	1 cut(s)	595
BseAI	TCCGGA	1 cut(s)	1776
BseBI	CCWGG	1 cut(s)	1506
BseCI	ATCGAT	1 cut(s)	1873
BseDI	CCNNGG	6 cut(s)	387, 887, 985, 1093, 1318, 1864
BseGI	GGATG	5 cut(s)	190, 421, 547, 1075, 1552
BseLI	CCNNNNNNNGG	3 cut(s)	161, 257, 1305
BseMI	GCAATG	1 cut(s)	595
BseMII	CTCAG	3 cut(s)	668, 764, 1197
BseNI	ACTGG	5 cut(s)	533, 682, 1158, 1680, 1888
BseRI	GAGGAG	3 cut(s)	306, 1055, 1955
BseSI	GKGCMC	1 cut(s)	1582
BseX3I	CGGCCG	1 cut(s)	342
BseXI	GCAGC	2 cut(s)	662, 1273
BseYI	CCCAGC	1 cut(s)	1698
Bsh1236I	CGCG	1 cut(s)	1866
Bsh1285I	CGRYCG	1 cut(s)	345
BshFI	GGCC	5 cut(s)	344, 604, 869, 1061, 1854
BshVI	ATCGAT	1 cut(s)	1873
BsiEI	CGRYCG	1 cut(s)	345
BsiHKAI	GWGCWC	3 cut(s)	110, 781, 1582
BsiSI	CCGG	4 cut(s)	258, 1777, 1801, 1851
BslFI	GGGAC	1 cut(s)	914
BslI	CCNNNNNNNGG	3 cut(s)	161, 257, 1305
BsmAI	GTCTC	4 cut(s)	753, 1193, 1864, 1884
BsmFI	GGGAC	1 cut(s)	914
BsmI	GAATGC	3 cut(s)	328, 1051, 1340
BsnI	GGCC	5 cut(s)	344, 604, 869, 1061, 1854
Bso31I	GGTCTC	1 cut(s)	1193
Bsp1286I	GDGCHC	4 cut(s)	110, 781, 1212, 1582
Bsp13I	TCCGGA	1 cut(s)	1776
Bsp1407I	TGTACA	2 cut(s)	1234, 1641
Bsp143I	GATC	6 cut(s)	286, 784, 994, 1797, 1870, 1874
Bsp19I	CCATGG	2 cut(s)	387, 887
BspANI	GGCC	5 cut(s)	344, 604, 869, 1061, 1854
BspCNI	CTCAG	3 cut(s)	667, 763, 1198
BspDI	ATCGAT	1 cut(s)	1873
BspEI	TCCGGA	1 cut(s)	1776
BspFNI	CGCG	1 cut(s)	1866
BspLI	GGNNCC	1 cut(s)	153
BspMAI	CTGCAG	1 cut(s)	432
BspPI	GGATC	3 cut(s)	779, 1792, 1878
BspQI	GCTCTTC	2 cut(s)	74, 546
BspTNI	GGTCTC	1 cut(s)	1193
BsrBI	CCGCTC	2 cut(s)	341, 667
BsrDI	GCAATG	1 cut(s)	595
BsrGI	TGTACA	2 cut(s)	1234, 1641
BsrI	ACTGG	5 cut(s)	533, 682, 1158, 1680, 1888
BssECI	CCNNGG	6 cut(s)	387, 887, 985, 1093, 1318, 1864
BssMI	GATC	6 cut(s)	286, 784, 994, 1797, 1870, 1874
BssNAI	GTATAC	1 cut(s)	1377
BssNI	GRCGYC	1 cut(s)	1944
BssT1I	CCWWGG	3 cut(s)	387, 887, 985
Bst1107I	GTATAC	1 cut(s)	1377
Bst2UI	CCWGG	1 cut(s)	1506
Bst6I	CTCTTC	2 cut(s)	74, 546
BstACI	GRCGYC	1 cut(s)	1944
BstAPI	GCANNNNNTGC	3 cut(s)	323, 741, 1822
BstAUI	TGTACA	2 cut(s)	1234, 1641
BstC8I	GCNNGC	7 cut(s)	214, 853, 1212, 1274, 1303, 1570, 1663
BstDEI	CTNAG	3 cut(s)	654, 750, 1206
BstDSI	CCRYGG	5 cut(s)	387, 887, 1093, 1318, 1864
BstF5I	GGATG	5 cut(s)	190, 421, 547, 1075, 1552
BstFNI	CGCG	1 cut(s)	1866
BstHHI	GCGC	1 cut(s)	1816
BstKTI	GATC	6 cut(s)	289, 787, 997, 1800, 1873, 1877
BstMAI	GTCTC	4 cut(s)	753, 1193, 1864, 1884
BstMBI	GATC	6 cut(s)	286, 784, 994, 1797, 1870, 1874
BstMCI	CGRYCG	1 cut(s)	345
BstMWI	GCNNNNNNNGC	6 cut(s)	323, 741, 1397, 1632, 1811, 1822
BstNI	CCWGG	1 cut(s)	1506
BstNSI	RCATGY	5 cut(s)	216, 1536, 1648, 1665, 1825
BstSCI	CCNGG	4 cut(s)	256, 1504, 1800, 1849
BstSFI	CTRYAG	2 cut(s)	428, 1392
BstSLI	GKGCMC	1 cut(s)	1582
BstUI	CGCG	1 cut(s)	1866
BstV1I	GCAGC	2 cut(s)	662, 1273
BstV2I	GAAGAC	2 cut(s)	203, 1940
BstX2I	RGATCY	1 cut(s)	1797
BstXI	CCANNNNNNTGG	2 cut(s)	208, 894
BstYI	RGATCY	1 cut(s)	1797
BstZ17I	GTATAC	1 cut(s)	1377
BstZI	CGGCCG	1 cut(s)	342
Bsu15I	ATCGAT	1 cut(s)	1873
BsuI	GTATCC	1 cut(s)	1303
BsuRI	GGCC	5 cut(s)	344, 604, 869, 1061, 1854
BsuTUI	ATCGAT	1 cut(s)	1873
BtgI	CCRYGG	5 cut(s)	387, 887, 1093, 1318, 1864
BtgZI	GCGATG	1 cut(s)	1383
BtsCI	GGATG	5 cut(s)	190, 421, 547, 1075, 1552
BtsIMutI	CAGTG	6 cut(s)	746, 758, 1203, 1253, 1381, 1687
Cac8I	GCNNGC	7 cut(s)	214, 853, 1212, 1274, 1303, 1570, 1663
CciNI	GCGGCCGC	1 cut(s)	342
CfoI	GCGC	1 cut(s)	1816
Cfr13I	GGNCC	2 cut(s)	602, 832
Cfr42I	CCGCGG	1 cut(s)	1867
ClaI	ATCGAT	1 cut(s)	1873
CspCI	CAANNNNNGTGG	2 cut(s)	859, 894
DdeI	CTNAG	3 cut(s)	654, 750, 1206
DpnI	GATC	6 cut(s)	288, 786, 996, 1799, 1872, 1876
DpnII	GATC	6 cut(s)	286, 784, 994, 1797, 1870, 1874
DraI	TTTAAA	1 cut(s)	643
EaeI	YGGCCR	3 cut(s)	342, 867, 1852
EagI	CGGCCG	1 cut(s)	342
Eam1104I	CTCTTC	2 cut(s)	74, 546
EarI	CTCTTC	2 cut(s)	74, 546
EciI	GGCGGA	1 cut(s)	1902
Ecl136II	GAGCTC	1 cut(s)	108
EclXI	CGGCCG	1 cut(s)	342
Eco130I	CCWWGG	3 cut(s)	387, 887, 985
Eco24I	GRGCYC	2 cut(s)	110, 1212
Eco31I	GGTCTC	1 cut(s)	1193
Eco47I	GGWCC	1 cut(s)	832
Eco52I	CGGCCG	1 cut(s)	342
Eco53kI	GAGCTC	1 cut(s)	108
Eco57I	CTGAAG	3 cut(s)	1019, 1050, 1076
EcoICRI	GAGCTC	1 cut(s)	108
EcoRII	CCWGG	1 cut(s)	1504
EcoT14I	CCWWGG	3 cut(s)	387, 887, 985
EcoT22I	ATGCAT	2 cut(s)	1663, 1667
EcoT38I	GRGCYC	2 cut(s)	110, 1212
ErhI	CCWWGG	3 cut(s)	387, 887, 985
FalI	AAGNNNNNCTT	2 cut(s)	71, 103
FaqI	GGGAC	1 cut(s)	914
FauI	CCCGC	3 cut(s)	1219, 1859, 1871
FblI	GTMKAC	1 cut(s)	1376
Fnu4HI	GCNGC	4 cut(s)	342, 345, 651, 1287
FokI	GGATG	5 cut(s)	177, 428, 534, 1082, 1559
FriOI	GRGCYC	2 cut(s)	110, 1212
Fsp4HI	GCNGC	4 cut(s)	342, 345, 651, 1287
FspBI	CTAG	2 cut(s)	524, 1695
GlaI	GCGC	1 cut(s)	1815
GluI	GCNGC	4 cut(s)	342, 345, 651, 1287
GsaI	CCCAGC	1 cut(s)	1702
HaeIII	GGCC	5 cut(s)	344, 604, 869, 1061, 1854
HapII	CCGG	4 cut(s)	258, 1777, 1801, 1851
HhaI	GCGC	1 cut(s)	1816
Hin1I	GRCGYC	1 cut(s)	1944
Hin6I	GCGC	1 cut(s)	1814
HinP1I	GCGC	1 cut(s)	1814
HincII	GTYRAC	1 cut(s)	514
HindII	GTYRAC	1 cut(s)	514
HindIII	AAGCTT	3 cut(s)	11, 720, 849
HinfI	GANTC	5 cut(s)	239, 980, 1509, 1773, 1790
HpaII	CCGG	4 cut(s)	258, 1777, 1801, 1851
HphI	GGTGA	3 cut(s)	485, 512, 1529
Hpy188I	TCNGA	9 cut(s)	105, 195, 207, 999, 1056, 1742, 1795, 1879, 1921
Hpy188III	TCNNGA	9 cut(s)	88, 398, 551, 716, 782, 788, 1472, 1777, 1948
Hpy99I	CGWCG	2 cut(s)	239, 1946
HpyAV	CCTTC	7 cut(s)	253, 307, 456, 664, 1051, 1429, 1832
HpyCH4IV	ACGT	5 cut(s)	559, 925, 1313, 1405, 1944
HpyF10VI	GCNNNNNNNGC	6 cut(s)	323, 741, 1397, 1632, 1811, 1822
HpyF3I	CTNAG	3 cut(s)	654, 750, 1206
HpySE526I	ACGT	5 cut(s)	559, 925, 1313, 1405, 1944
Hsp92I	GRCGYC	1 cut(s)	1944
HspAI	GCGC	1 cut(s)	1814
Kpn2I	TCCGGA	1 cut(s)	1776
KspI	CCGCGG	1 cut(s)	1867
Kzo9I	GATC	6 cut(s)	286, 784, 994, 1797, 1870, 1874
LguI	GCTCTTC	2 cut(s)	74, 546
LmnI	GCTCC	5 cut(s)	338, 647, 784, 1163, 1397
Lsp1109I	GCAGC	2 cut(s)	662, 1273
LweI	GCATC	5 cut(s)	93, 406, 1060, 1648, 1902
MaeI	CTAG	2 cut(s)	524, 1695
MaeII	ACGT	5 cut(s)	559, 925, 1313, 1405, 1944
MaeIII	GTNAC	6 cut(s)	94, 299, 473, 907, 1102, 1610
MalI	GATC	6 cut(s)	288, 786, 996, 1799, 1872, 1876
MbiI	CCGCTC	2 cut(s)	341, 667
MboI	GATC	6 cut(s)	286, 784, 994, 1797, 1870, 1874
MflI	RGATCY	1 cut(s)	1797
MhlI	GDGCHC	4 cut(s)	110, 781, 1212, 1582
MlsI	TGGCCA	1 cut(s)	869
MluCI	AATT	3 cut(s)	441, 1413, 1707
MluNI	TGGCCA	1 cut(s)	869
MmeI	TCCRAC	2 cut(s)	1412, 1720
Mox20I	TGGCCA	1 cut(s)	869
Mph1103I	ATGCAT	2 cut(s)	1663, 1667
MroI	TCCGGA	1 cut(s)	1776
MscI	TGGCCA	1 cut(s)	869
MseI	TTAA	4 cut(s)	642, 1223, 1416, 1706
MslI	CAYNNNNRTG	1 cut(s)	552
Msp20I	TGGCCA	1 cut(s)	869
MspA1I	CMGCKG	2 cut(s)	1214, 1866
MspI	CCGG	4 cut(s)	258, 1777, 1801, 1851
MspR9I	CCNGG	4 cut(s)	258, 1506, 1802, 1851
Mva1269I	GAATGC	3 cut(s)	328, 1051, 1340
MvaI	CCWGG	1 cut(s)	1506
MvnI	CGCG	1 cut(s)	1866
MwoI	GCNNNNNNNGC	6 cut(s)	323, 741, 1397, 1632, 1811, 1822
NciI	CCSGG	3 cut(s)	258, 1802, 1851
NcoI	CCATGG	2 cut(s)	387, 887
NdeII	GATC	6 cut(s)	286, 784, 994, 1797, 1870, 1874
NlaIV	GGNNCC	1 cut(s)	153
NmeAIII	GCCGAG	1 cut(s)	1428
NmuCI	GTSAC	3 cut(s)	94, 473, 907
NotI	GCGGCCGC	1 cut(s)	342
NsiI	ATGCAT	2 cut(s)	1663, 1667
NspI	RCATGY	5 cut(s)	216, 1536, 1648, 1665, 1825
PaeI	GCATGC	2 cut(s)	216, 1665
PciI	ACATGT	1 cut(s)	1532
PciSI	GCTCTTC	2 cut(s)	74, 546
PctI	GAATGC	3 cut(s)	328, 1051, 1340
PfeI	GAWTC	5 cut(s)	239, 980, 1509, 1773, 1790
PflFI	GACNNNGTC	1 cut(s)	905
PkrI	GCNGC	4 cut(s)	343, 346, 652, 1288
PscI	ACATGT	1 cut(s)	1532
PshBI	ATTAAT	1 cut(s)	1706
Psp124BI	GAGCTC	1 cut(s)	110
Psp6I	CCWGG	1 cut(s)	1504
PspFI	CCCAGC	1 cut(s)	1698
PspGI	CCWGG	1 cut(s)	1504
PspN4I	GGNNCC	1 cut(s)	153
PspPI	GGNCC	2 cut(s)	602, 832
PstI	CTGCAG	1 cut(s)	432
PsuI	RGATCY	1 cut(s)	1797
PsyI	GACNNNGTC	1 cut(s)	905
RseI	CAYNNNNRTG	1 cut(s)	552
SacI	GAGCTC	1 cut(s)	110
SacII	CCGCGG	1 cut(s)	1867
SapI	GCTCTTC	2 cut(s)	74, 546
SaqAI	TTAA	4 cut(s)	642, 1223, 1416, 1706
SatI	GCNGC	4 cut(s)	342, 345, 651, 1287
Sau3AI	GATC	6 cut(s)	286, 784, 994, 1797, 1870, 1874
Sau96I	GGNCC	2 cut(s)	602, 832
ScrFI	CCNGG	4 cut(s)	258, 1506, 1802, 1851
SduI	GDGCHC	4 cut(s)	110, 781, 1212, 1582
SfaNI	GCATC	5 cut(s)	93, 406, 1060, 1648, 1902
SfcI	CTRYAG	2 cut(s)	428, 1392
Sfr303I	CCGCGG	1 cut(s)	1867
SgrBI	CCGCGG	1 cut(s)	1867
SinI	GGWCC	1 cut(s)	832
SmiMI	CAYNNNNRTG	1 cut(s)	552
SmlI	CTYRAG	1 cut(s)	662
SmoI	CTYRAG	1 cut(s)	662
SphI	GCATGC	2 cut(s)	216, 1665
Sse9I	AATT	3 cut(s)	441, 1413, 1707
SspI	AATATT	1 cut(s)	1110
SspMI	CTAG	2 cut(s)	524, 1695
SstI	GAGCTC	1 cut(s)	110
StyD4I	CCNGG	4 cut(s)	256, 1504, 1800, 1849
StyI	CCWWGG	3 cut(s)	387, 887, 985
TaiI	ACGT	5 cut(s)	562, 928, 1316, 1408, 1947
TaqI	TCGA	5 cut(s)	110, 289, 731, 1771, 1873
TasI	AATT	3 cut(s)	441, 1413, 1707
TatI	WGTACW	5 cut(s)	948, 1140, 1234, 1641, 1818
TauI	GCSGC	2 cut(s)	344, 347
TfiI	GAWTC	5 cut(s)	239, 980, 1509, 1773, 1790
Tru1I	TTAA	4 cut(s)	642, 1223, 1416, 1706
Tru9I	TTAA	4 cut(s)	642, 1223, 1416, 1706
TscAI	CASTG	6 cut(s)	746, 758, 1210, 1253, 1388, 1687
TseFI	GTSAC	3 cut(s)	94, 473, 907
TseI	GCWGC	2 cut(s)	650, 1286
Tsp45I	GTSAC	3 cut(s)	94, 473, 907
TspDTI	ATGAA	6 cut(s)	17, 570, 888, 950, 1851, 1888
TspGWI	ACGGA	5 cut(s)	177, 548, 833, 926, 1307
TspRI	CASTG	6 cut(s)	746, 758, 1210, 1253, 1388, 1687
Tth111I	GACNNNGTC	1 cut(s)	905
VneI	GTGCAC	1 cut(s)	1578
VpaK11BI	GGWCC	1 cut(s)	832
VspI	ATTAAT	1 cut(s)	1706
XceI	RCATGY	5 cut(s)	216, 1536, 1648, 1665, 1825
XcmI	CCANNNNNNNNNTGG	1 cut(s)	894
XmiI	GTMKAC	1 cut(s)	1376
XspI	CTAG	2 cut(s)	524, 1695
ZraI	GACGTC	1 cut(s)	1945
Zsp2I	ATGCAT	2 cut(s)	1663, 1667

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.