Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0003973

Physical Location & Seq

Genomic Coordinates Reverse (-)

22534147 .. 22538134

Gene Structure 3988 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2775 bp

ATGGAAACTATGGTGTCCGGTTCAGATGAACCGGAGTTGAAGCATGGGAATCTGGTCATGCAGTGGAAGCATCTGTTGGACCACATTGACTTGCTCCAGACCCAGTTTGAAGAGCTTCAGGAACGGTTCGACTCGGAGTGGAACTCACTCCGGACCCGGCAGCGGGAGGTTGAGAAGCGCGAGGAGGAGTTGGGGGCTAAAGTTCTGAACTTTCAATCGGAATTGGAGTCCAAAGCTCAGGAATTGTATGAAATCGAGAGAATGGCGAAAGAGAAAAGAAGAGAGGTTGGTGGTAAGGAGAAATGGTTGTTGGAAATTGAGGAGTTGGTTAGGGGAAAGGAGAGGGAACGTGATTTGATAAAAAATGATATCGAAGAGCAAACGGAGAAATTGAATTCGGTTGTGAAAAGTTTGGATGAGAAGTCTAGGGAGCTGAAGGTGAAGAAGGAGGTCACTGAAGTGAACGGAAGGCGATTAAATGCGATGAAGGGGTCTATTGATGAGACCGTGAAAGATTTGGTTTCGAAACAAGAGCAGGTTAAAGCGGTGCAGAAATCTCTCGAAGAATGTCACAAGGAGATTCAATTGAAAAAGGTGTTACTTGGTGGCCTTGAGAAATCGATTAGGGAATGTTACAGTACACTCGAATCTAAAGAGAAAACCATCAGGGCAATGGAGTTGAAACAAAAACAATTTGAGTTGGTGGTTCAAGAGTGCCAAAAGCCTTTGGATTCAAAAGAAAAGTTGTTGCTAGAAGGTTGCCATGGACTCGAAATGAAGGAGAGGCAACTTGAAGAACAGGTCAGAGTGCTTGAATCGAAACAAAAACAATTTGAGTTGATGGTTCAAGAGCGCCAAAAGCATTTTGATTCAAAAGAAAAGTTGTTGCAAGAAGGTTTCCATGGACTCGAAATGAAAGAAGGGCGACTTGAAGAACAGGTCAGAGAATTTGAATCGAAACAAAAACAATTTGATTTGACGATTCAAGAACGCCGACAAATTTTGGATTCACAAGAGAAGTTGTTACAAGAAGGTTCCCATGGAGTTAAAACGAAAGAGAGGCAGCTTGAAGAACAGGTCAGAGAGCTCGAATCAAAACAAAAACAATTTGAGTTGCTGCAAAAATCCAAGGAAGACATCCAAAATCTGAAATCCAAGGAGAAGAATAATTGTTCCTGGGATGGCAGATGCTTGCAGTTGCTCATGAATGAGCACTGCACGAGAACCGATTTTGTATGTAGTGAAATTGAAGCTGTTCTTCAGGCATCATCTGACCCAGCAAAGCTCGTTTTGGATGCAATGCAAGGGTTTTACCCTTCAAATTGGACCGTGGACACCACTGAGTTTGTCTATGATTTGACAGTTATAAGGAGGAGTTGTATTCTTTTGTTACAGCAGTTGAAGAGATTCTCACTACAAATCAATCCTCAGGTGAGAAAAGAAGCAGGGGCTTTGGCGGCTGAATGGAAGGCTAAGATGACTGTGGCAACCGAGAATGGATTGGAGATTTTGGGTTTTCTGGAGCTTGTTTCTGTGTATGAAATCACAACTGTTTATGATTCGAAGGAGCTTCAGAGTTTTCTTGGTATAGTGGCTCAGCACGAGCAAGGAACTGAGCTATGCCAGGCCCTTGGTATCACAGATAGGGCACCTGGTAAGCTCTTTTCCAGTAACAGCATTTCTTTGCCTATCAAAATTGAGGAACCAGAATTTTCACCTGTTATAAATGTAGCAATTTCATCTTCTCTGGATCTTCAACCGAATGCAAACACAGATGCAAGGAATTTGCAGGGCTGTACAAATGAGCATTTAAGTGAGAACGAGTCAGTACAGACCAAAATGCTTTTTAATCTACAAATATCATTGGACCCAGGAGAAGTTGTTTTGAAGTTGATAAAAGAATCTTTGGCTCAGTGCTGGAGAAAAGGAGATGTTGGTTGTGAATCTGCTGTCATGGAGGATAGCATTTCCCTGTTGAATGATCTAATGGATGTCTCCACGCCTATTGGACCAAATGTGATAAAAAATGCAAAAGAGCTAGCAGTCCAGTGGAAAGCAAAAATGAGAGTTGATTCTGAAAATTCAATGGAGATTTTGGGGTTTTTACAGTTTATAGTTACATATGGATTGGTTTCTACCTTTAACAGAGATGAGATTGTAAAGCTTCTTGGGACGATTTGTCAGCATAAAAAGGCCTTGGAATCATGCCAGGAAATTGGTTTTGCAGATATGATCCCTGTTATAATTTGCAATTCTCCTTACGGAAATTCTCTGGAGCTGTTTGATGAATCCGTCAATTTTGTTTTACATGCTGCAGATTTTGTTCAGGATTTTATTGAAAGGAAGCAACTGATTGAAGCTGTCAGCTTGATTTGCACCTTCAAGTTAACTGACAGGTTCCCGCCGGTGTTACTCTTAAAAGAATACGTGGAAGTTGCAAGGAAGTCTTACTGCACAAAATGGCTGGTAAAAAGATCACTTGATGAAAAGAATGAGGTGCTAGACGACCACATAGCTGGTCTGAAGGCTGCGATTCAATGTATCAAAGACAACAACCTCGAGCCAGAATTCCCATACAAGGACATCGAAAAAGAGATAGTGAAGCTGGAAAAGCATAAGCTGAATTGGAGATGCTCGGTTCCACTTGCTGCCTTCAAGGTTCAACGAGAGCCCGAGAAAGGGAAGAAACCTGATACCACCCGTGTATCCTTCAAGATCAAACCGCCAGAACAGCGCAACAGTAAGCGCCTCAAACGGGGTGGCCTGATGGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Frigida

Protein Analysis

925

Amino Acids

106.54

Weight (kDa)

5.55

Isoelectric Point (pI)

50.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Frigida	PF07899	393 - 552	2.8e-30	Frigida-like protein
Frigida	PF07899	591 - 749	1.3e-22	Frigida-like protein
Frigida	PF07899	764 - 875	5.6e-24	Frigida-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27220 AT5G27220 AT5G27220 AT5G27220
fragaria_vesca	FvH4_6g04390
malus_domestica	MD04G1214300.v1.1 MD04G1214400.v1.1 MD04G1214500.v1.1 MD12G1228900.v1.1 MD12G1229100.v1.1
prunus_persica	Prupe.6G334100_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334400_v2.0.a1 Prupe.6G334500_v2.0.a1
pyrus_communis	pycom04g18950 pycom12g21350 pycom12g21360
rosa_chinensis	RchiOBHm_Chr3g0453001
rosa_laevigata	RLG00000025551
rosa_multiflora	Rmu_co8146304.1_g000001 Rmu_co8342113.1_g000001 Rmu_sc0005888.1_g000003
rosa_roxburghii	Rroxscaffold_6G00430100
rosa_rugosa	Rorug02G0645100 Rorug02G0645200 Rorug02G0645300
rosa_samantha	Rh3AG047500 Rh3BG050000 Rh3CG048800 Rh3DG049600
rosa_wichuraiana	Rw3G003720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1367, 1724, 2243
Acc36I	ACCTGC	1 cut(s)	526
AccB1I	GGYRCC	1 cut(s)	1648
AccII	CGCG	1 cut(s)	180
AccIII	TCCGGA	1 cut(s)	150
AciI	CCGC	5 cut(s)	163, 545, 1457, 2402, 2723
AclWI	GGATC	2 cut(s)	1758, 2227
AcsI	RAATTY	8 cut(s)	394, 947, 999, 1709, 1783, 2080, 2266, 2567
AcuI	CTGAAG	6 cut(s)	101, 455, 477, 1244, 1556, 2543
AfaI	GTAC	3 cut(s)	640, 1798, 1830
AfiI	CCNNNNNNNGG	6 cut(s)	162, 163, 2578, 2678, 2679, 2755
AhdI	GACNNNNNGTC	1 cut(s)	2178
AjnI	CCWGG	5 cut(s)	1175, 1623, 1651, 1870, 2208
AjuI	GAANNNNNNNTTGG	4 cut(s)	59, 91, 293, 325
AleI	CACNNNNGTG	1 cut(s)	458
Alw21I	GWGCWC	2 cut(s)	1089, 1215
Alw26I	GTCTC	2 cut(s)	497, 1999
AlwI	GGATC	2 cut(s)	1758, 2227
Ama87I	CYCGRG	2 cut(s)	2558, 2672
Aor13HI	TCCGGA	1 cut(s)	150
AoxI	GGCC	4 cut(s)	607, 1626, 2193, 2761
ApeKI	GCWGC	6 cut(s)	160, 1063, 1117, 2312, 2528, 2648
ApoI	RAATTY	8 cut(s)	394, 947, 999, 1709, 1783, 2080, 2266, 2567
Asp700I	GAANNNNTTC	3 cut(s)	114, 1254, 1406
AspLEI	GCGC	4 cut(s)	180, 855, 2736, 2748
AspS9I	GGNCC	6 cut(s)	79, 153, 1326, 1627, 1867, 2009
AsuC2I	CCSGG	1 cut(s)	157
AsuHPI	GGTGA	3 cut(s)	451, 1444, 1707
AsuII	TTCGAA	2 cut(s)	524, 1562
AsuNHI	GCTAGC	1 cut(s)	2038
AvaI	CYCGRG	2 cut(s)	2558, 2672
AvaII	GGWCC	5 cut(s)	79, 153, 1326, 1867, 2009
AxyI	CCTNAGG	1 cut(s)	1428
BaeGI	GKGCMC	1 cut(s)	1651
BaeI	ACNNNNGTAYC	2 cut(s)	2688, 2721
BanI	GGYRCC	1 cut(s)	1648
BanII	GRGCYC	2 cut(s)	1089, 2673
BauI	CACGAG	2 cut(s)	1219, 1601
BbsI	GAAGAC	1 cut(s)	1140
Bbv12I	GWGCWC	2 cut(s)	1089, 1215
BbvI	GCAGC	6 cut(s)	172, 1075, 1104, 2299, 2515, 2635
BccI	CCATC	4 cut(s)	671, 835, 1175, 2761
BciT130I	CCWGG	5 cut(s)	1177, 1625, 1653, 1872, 2210
BciVI	GTATCC	1 cut(s)	2716
BcnI	CCSGG	1 cut(s)	157
BcoDI	GTCTC	2 cut(s)	497, 1999
BfaI	CTAG	5 cut(s)	426, 752, 2039, 2501, 2773
BfmI	CTRYAG	1 cut(s)	2313
BfoI	RGCGCY	2 cut(s)	856, 2749
BfuAI	ACCTGC	1 cut(s)	526
BfuI	GTATCC	1 cut(s)	2716
BisI	GCNGC	7 cut(s)	161, 1064, 1118, 1458, 2313, 2529, 2649
BlpI	GCTNAGC	1 cut(s)	1596
BlsI	GCNGC	7 cut(s)	162, 1065, 1119, 1459, 2314, 2530, 2650
Bme1390I	CCNGG	6 cut(s)	157, 1177, 1625, 1653, 1872, 2210
Bme18I	GGWCC	5 cut(s)	79, 153, 1326, 1867, 2009
BmeRI	GACNNNNNGTC	1 cut(s)	2178
BmeT110I	CYCGRG	2 cut(s)	2558, 2672
BmgT120I	GGNCC	6 cut(s)	79, 153, 1326, 1627, 1867, 2009
BmiI	GGNNCC	7 cut(s)	155, 1036, 1650, 1704, 1869, 2399, 2640
BmrFI	CCNGG	6 cut(s)	157, 1177, 1625, 1653, 1872, 2210
BmrI	ACTGGG	1 cut(s)	97
BmsI	GCATC	6 cut(s)	79, 1178, 1274, 1285, 1765, 2621
BmtI	GCTAGC	1 cut(s)	2042
BmuI	ACTGGG	1 cut(s)	97
BpiI	GAAGAC	1 cut(s)	1140
BplI	GAGNNNNNCTC	2 cut(s)	128, 160
BpmI	CTGGAG	4 cut(s)	80, 1541, 1939, 2294
Bpu10I	CCTNAGC	1 cut(s)	237
Bpu1102I	GCTNAGC	1 cut(s)	1596
Bpu14I	TTCGAA	2 cut(s)	524, 1562
BpuEI	CTTGAG	1 cut(s)	632
BpuMI	CCSGG	1 cut(s)	157
Bsa29I	ATCGAT	1 cut(s)	620
BsaAI	YACGTR	1 cut(s)	2428
BsaI	GGTCTC	1 cut(s)	497
BsaWI	WCCGGW	3 cut(s)	17, 31, 150
BsaXI	ACNNNNNCTCC	6 cut(s)	290, 320, 1363, 1393, 1866, 1896
Bsc4I	CCNNNNNNNGG	6 cut(s)	162, 163, 2578, 2678, 2679, 2755
Bse118I	RCCGGY	1 cut(s)	2404
Bse1I	ACTGG	3 cut(s)	103, 1668, 2047
Bse21I	CCTNAGG	1 cut(s)	1428
Bse3DI	GCAATG	2 cut(s)	678, 1305
BseAI	TCCGGA	1 cut(s)	150
BseBI	CCWGG	5 cut(s)	1177, 1625, 1653, 1872, 2210
BseCI	ATCGAT	1 cut(s)	620
BseGI	GGATG	5 cut(s)	421, 1137, 1186, 1300, 1996
BseLI	CCNNNNNNNGG	6 cut(s)	162, 163, 2578, 2678, 2679, 2755
BseMI	GCAATG	2 cut(s)	678, 1305
BseMII	CTCAG	6 cut(s)	251, 1332, 1442, 1605, 1610, 1925
BseNI	ACTGG	3 cut(s)	103, 1668, 2047
BseRI	GAGGAG	4 cut(s)	197, 200, 335, 1387
BseSI	GKGCMC	1 cut(s)	1651
BseXI	GCAGC	6 cut(s)	172, 1075, 1104, 2299, 2515, 2635
BseYI	CCCAGC	1 cut(s)	1276
BsgI	GTGCAG	3 cut(s)	569, 1201, 2437
Bsh1236I	CGCG	1 cut(s)	180
BshFI	GGCC	4 cut(s)	609, 1628, 2195, 2763
BshNI	GGYRCC	1 cut(s)	1648
BshVI	ATCGAT	1 cut(s)	620
BsiHKAI	GWGCWC	2 cut(s)	1089, 1215
BsiHKCI	CYCGRG	2 cut(s)	2558, 2672
BsiSI	CCGG	5 cut(s)	18, 32, 151, 157, 2405
BslFI	GGGAC	1 cut(s)	2185
BslI	CCNNNNNNNGG	6 cut(s)	162, 163, 2578, 2678, 2679, 2755
BsmAI	GTCTC	2 cut(s)	497, 1999
BsmFI	GGGAC	1 cut(s)	2185
BsmI	GAATGC	1 cut(s)	1768
BsnI	GGCC	4 cut(s)	609, 1628, 2195, 2763
Bso31I	GGTCTC	1 cut(s)	497
BsoBI	CYCGRG	2 cut(s)	2558, 2672
Bsp119I	TTCGAA	2 cut(s)	524, 1562
Bsp1286I	GDGCHC	4 cut(s)	1089, 1215, 1651, 2673
Bsp13I	TCCGGA	1 cut(s)	150
Bsp1407I	TGTACA	1 cut(s)	1796
Bsp143I	GATC	5 cut(s)	1750, 1981, 2232, 2474, 2715
Bsp1720I	GCTNAGC	1 cut(s)	1596
Bsp19I	CCATGG	3 cut(s)	763, 901, 1039
BspACI	CCGC	5 cut(s)	163, 545, 1457, 2402, 2723
BspANI	GGCC	4 cut(s)	609, 1628, 2195, 2763
BspCNI	CTCAG	6 cut(s)	250, 1333, 1441, 1606, 1609, 1924
BspDI	ATCGAT	1 cut(s)	620
BspEI	TCCGGA	1 cut(s)	150
BspFNI	CGCG	1 cut(s)	180
BspHI	TCATGA	1 cut(s)	1203
BspLI	GGNNCC	7 cut(s)	155, 1036, 1650, 1704, 1869, 2399, 2640
BspMAI	CTGCAG	1 cut(s)	2317
BspMI	ACCTGC	1 cut(s)	526
BspOI	GCTAGC	1 cut(s)	2042
BspPI	GGATC	2 cut(s)	1758, 2227
BspQI	GCTCTTC	2 cut(s)	105, 369
BspT104I	TTCGAA	2 cut(s)	524, 1562
BspT107I	GGYRCC	1 cut(s)	1648
BspTNI	GGTCTC	1 cut(s)	497
BsrDI	GCAATG	2 cut(s)	678, 1305
BsrFI	RCCGGY	1 cut(s)	2404
BsrGI	TGTACA	1 cut(s)	1796
BsrI	ACTGG	3 cut(s)	103, 1668, 2047
BssAI	RCCGGY	1 cut(s)	2404
BssMI	GATC	5 cut(s)	1750, 1981, 2232, 2474, 2715
BssSI	CACGAG	2 cut(s)	1219, 1601
BssT1I	CCWWGG	7 cut(s)	763, 901, 1039, 1128, 1155, 1630, 2196
Bst2BI	CACGAG	2 cut(s)	1219, 1601
Bst2UI	CCWGG	5 cut(s)	1177, 1625, 1653, 1872, 2210
Bst4CI	ACNGT	9 cut(s)	126, 508, 638, 1330, 1363, 1483, 1552, 2109, 2741
Bst6I	CTCTTC	4 cut(s)	105, 274, 369, 1397
BstAUI	TGTACA	1 cut(s)	1796
BstBAI	YACGTR	1 cut(s)	2428
BstBI	TTCGAA	2 cut(s)	524, 1562
BstC8I	GCNNGC	2 cut(s)	1193, 2040
BstDEI	CTNAG	7 cut(s)	237, 1341, 1428, 1473, 1596, 1614, 1911
BstDSI	CCRYGG	4 cut(s)	763, 901, 1039, 1329
BstF5I	GGATG	5 cut(s)	421, 1137, 1186, 1300, 1996
BstFNI	CGCG	1 cut(s)	180
BstH2I	RGCGCY	2 cut(s)	856, 2749
BstHHI	GCGC	4 cut(s)	180, 855, 2736, 2748
BstKTI	GATC	5 cut(s)	1753, 1984, 2235, 2477, 2718
BstMAI	GTCTC	2 cut(s)	497, 1999
BstMBI	GATC	5 cut(s)	1750, 1981, 2232, 2474, 2715
BstMWI	GCNNNNNNNGC	6 cut(s)	67, 859, 1457, 2373, 2611, 2731
BstNI	CCWGG	5 cut(s)	1177, 1625, 1653, 1872, 2210
BstNSI	RCATGY	1 cut(s)	2312
BstSCI	CCNGG	6 cut(s)	155, 1175, 1623, 1651, 1870, 2208
BstSFI	CTRYAG	1 cut(s)	2313
BstSLI	GKGCMC	1 cut(s)	1651
BstUI	CGCG	1 cut(s)	180
BstV1I	GCAGC	6 cut(s)	172, 1075, 1104, 2299, 2515, 2635
BstV2I	GAAGAC	1 cut(s)	1140
BstX2I	RGATCY	1 cut(s)	1750
BstXI	CCANNNNNNTGG	3 cut(s)	1631, 2216, 2516
BstYI	RGATCY	1 cut(s)	1750
Bsu15I	ATCGAT	1 cut(s)	620
Bsu36I	CCTNAGG	1 cut(s)	1428
BsuI	GTATCC	1 cut(s)	2716
BsuRI	GGCC	4 cut(s)	609, 1628, 2195, 2763
BsuTUI	ATCGAT	1 cut(s)	620
BtgI	CCRYGG	4 cut(s)	763, 901, 1039, 1329
BtgZI	GCGATG	1 cut(s)	497
BtsCI	GGATG	5 cut(s)	421, 1137, 1186, 1300, 1996
BtsI	GCAGTG	2 cut(s)	68, 1213
BtsIMutI	CAGTG	6 cut(s)	68, 453, 1213, 1338, 1919, 2054
BveI	ACCTGC	1 cut(s)	526
Cac8I	GCNNGC	2 cut(s)	1193, 2040
CciI	TCATGA	1 cut(s)	1203
CfoI	GCGC	4 cut(s)	180, 855, 2736, 2748
Cfr10I	RCCGGY	1 cut(s)	2404
Cfr13I	GGNCC	6 cut(s)	79, 153, 1326, 1627, 1867, 2009
ClaI	ATCGAT	1 cut(s)	620
CsiI	ACCWGGT	1 cut(s)	1651
Csp6I	GTAC	3 cut(s)	639, 1797, 1829
CspCI	CAANNNNNGTGG	6 cut(s)	1327, 1362, 1987, 2022, 2740, 2775
CviAII	CATG	9 cut(s)	44, 58, 764, 902, 1040, 1204, 1954, 2205, 2309
CviQI	GTAC	3 cut(s)	639, 1797, 1829
DdeI	CTNAG	7 cut(s)	237, 1341, 1428, 1473, 1596, 1614, 1911
DpnI	GATC	5 cut(s)	1752, 1983, 2234, 2476, 2717
DpnII	GATC	5 cut(s)	1750, 1981, 2232, 2474, 2715
DriI	GACNNNNNGTC	1 cut(s)	2178
Eam1104I	CTCTTC	4 cut(s)	105, 274, 369, 1397
Eam1105I	GACNNNNNGTC	1 cut(s)	2178
EarI	CTCTTC	4 cut(s)	105, 274, 369, 1397
Ecl136II	GAGCTC	1 cut(s)	1087
Eco130I	CCWWGG	7 cut(s)	763, 901, 1039, 1128, 1155, 1630, 2196
Eco147I	AGGCCT	1 cut(s)	2195
Eco24I	GRGCYC	2 cut(s)	1089, 2673
Eco31I	GGTCTC	1 cut(s)	497
Eco32I	GATATC	1 cut(s)	370
Eco47I	GGWCC	5 cut(s)	79, 153, 1326, 1867, 2009
Eco53kI	GAGCTC	1 cut(s)	1087
Eco57I	CTGAAG	6 cut(s)	101, 455, 477, 1244, 1556, 2543
Eco81I	CCTNAGG	1 cut(s)	1428
Eco88I	CYCGRG	2 cut(s)	2558, 2672
EcoICRI	GAGCTC	1 cut(s)	1087
EcoO109I	RGGNCCY	1 cut(s)	1627
EcoRI	GAATTC	2 cut(s)	394, 2567
EcoRII	CCWGG	5 cut(s)	1175, 1623, 1651, 1870, 2208
EcoRV	GATATC	1 cut(s)	370
EcoT14I	CCWWGG	7 cut(s)	763, 901, 1039, 1128, 1155, 1630, 2196
EcoT38I	GRGCYC	2 cut(s)	1089, 2673
ErhI	CCWWGG	7 cut(s)	763, 901, 1039, 1128, 1155, 1630, 2196
FaeI	CATG	9 cut(s)	47, 61, 767, 905, 1043, 1207, 1957, 2208, 2312
FalI	AAGNNNNNCTT	6 cut(s)	912, 944, 1242, 1274, 2431, 2463
FaqI	GGGAC	1 cut(s)	2185
FatI	CATG	9 cut(s)	43, 57, 763, 901, 1039, 1203, 1953, 2204, 2308
FauI	CCCGC	2 cut(s)	156, 2409
FauNDI	CATATG	1 cut(s)	2122
Fnu4HI	GCNGC	7 cut(s)	161, 1064, 1118, 1458, 2313, 2529, 2649
FokI	GGATG	5 cut(s)	428, 1124, 1193, 1307, 2003
FriOI	GRGCYC	2 cut(s)	1089, 2673
Fsp4HI	GCNGC	7 cut(s)	161, 1064, 1118, 1458, 2313, 2529, 2649
FspBI	CTAG	5 cut(s)	426, 752, 2039, 2501, 2773
GlaI	GCGC	4 cut(s)	179, 854, 2735, 2747
GluI	GCNGC	7 cut(s)	161, 1064, 1118, 1458, 2313, 2529, 2649
GsaI	CCCAGC	1 cut(s)	1280
GsuI	CTGGAG	4 cut(s)	80, 1541, 1939, 2294
HaeII	RGCGCY	2 cut(s)	856, 2749
HaeIII	GGCC	4 cut(s)	609, 1628, 2195, 2763
HapII	CCGG	5 cut(s)	18, 32, 151, 157, 2405
HhaI	GCGC	4 cut(s)	180, 855, 2736, 2748
Hin1II	CATG	9 cut(s)	47, 61, 767, 905, 1043, 1207, 1957, 2208, 2312
Hin6I	GCGC	4 cut(s)	178, 853, 2734, 2746
HinP1I	GCGC	4 cut(s)	178, 853, 2734, 2746
HincII	GTYRAC	1 cut(s)	2388
HindII	GTYRAC	1 cut(s)	2388
HindIII	AAGCTT	1 cut(s)	2162
HpaI	GTTAAC	1 cut(s)	2388
HpaII	CCGG	5 cut(s)	18, 32, 151, 157, 2405
HphI	GGTGA	3 cut(s)	451, 1444, 1707
Hpy166II	GTNNAC	4 cut(s)	463, 641, 1333, 2388
Hpy8I	GTNNAC	4 cut(s)	463, 641, 1333, 2388
HpyCH4III	ACNGT	9 cut(s)	126, 508, 638, 1330, 1363, 1483, 1552, 2109, 2741
HpyCH4IV	ACGT	2 cut(s)	349, 2427
HpyF10VI	GCNNNNNNNGC	6 cut(s)	67, 859, 1457, 2373, 2611, 2731
HpyF3I	CTNAG	7 cut(s)	237, 1341, 1428, 1473, 1596, 1614, 1911
HpySE526I	ACGT	2 cut(s)	349, 2427
Hsp92II	CATG	9 cut(s)	47, 61, 767, 905, 1043, 1207, 1957, 2208, 2312
HspAI	GCGC	4 cut(s)	178, 853, 2734, 2746
Kpn2I	TCCGGA	1 cut(s)	150
KspAI	GTTAAC	1 cut(s)	2388
Kzo9I	GATC	5 cut(s)	1750, 1981, 2232, 2474, 2715
LguI	GCTCTTC	2 cut(s)	105, 369
LmnI	GCTCC	5 cut(s)	99, 430, 1522, 1567, 2275
Lsp1109I	GCAGC	6 cut(s)	172, 1075, 1104, 2299, 2515, 2635
LweI	GCATC	6 cut(s)	79, 1178, 1274, 1285, 1765, 2621
MabI	ACCWGGT	1 cut(s)	1651
MaeI	CTAG	5 cut(s)	426, 752, 2039, 2501, 2773
MaeII	ACGT	2 cut(s)	349, 2427
MaeIII	GTNAC	9 cut(s)	451, 569, 597, 632, 1023, 1389, 1670, 2116, 2409
MalI	GATC	5 cut(s)	1752, 1983, 2234, 2476, 2717
MboI	GATC	5 cut(s)	1750, 1981, 2232, 2474, 2715
MfeI	CAATTG	1 cut(s)	584
MflI	RGATCY	1 cut(s)	1750
MhlI	GDGCHC	4 cut(s)	1089, 1215, 1651, 2673
MlyI	GAGTC	5 cut(s)	125, 236, 762, 900, 1832
MmeI	TCCRAC	2 cut(s)	57, 291
MroI	TCCGGA	1 cut(s)	150
MroXI	GAANNNNTTC	3 cut(s)	114, 1254, 1406
MseI	TTAA	8 cut(s)	476, 540, 1047, 1811, 1848, 2142, 2387, 2417
MslI	CAYNNNNRTG	2 cut(s)	458, 1812
MspA1I	CMGCKG	1 cut(s)	163
MspI	CCGG	5 cut(s)	18, 32, 151, 157, 2405
MspR9I	CCNGG	6 cut(s)	157, 1177, 1625, 1653, 1872, 2210
MunI	CAATTG	1 cut(s)	584
Mva1269I	GAATGC	1 cut(s)	1768
MvaI	CCWGG	5 cut(s)	1177, 1625, 1653, 1872, 2210
MvnI	CGCG	1 cut(s)	180
MwoI	GCNNNNNNNGC	6 cut(s)	67, 859, 1457, 2373, 2611, 2731
NciI	CCSGG	1 cut(s)	157
NcoI	CCATGG	3 cut(s)	763, 901, 1039
NdeI	CATATG	1 cut(s)	2122
NdeII	GATC	5 cut(s)	1750, 1981, 2232, 2474, 2715
NheI	GCTAGC	1 cut(s)	2038
NlaIII	CATG	9 cut(s)	47, 61, 767, 905, 1043, 1207, 1957, 2208, 2312
NlaIV	GGNNCC	7 cut(s)	155, 1036, 1650, 1704, 1869, 2399, 2640
NmuCI	GTSAC	2 cut(s)	451, 569
NspI	RCATGY	1 cut(s)	2312
NspV	TTCGAA	2 cut(s)	524, 1562
OliI	CACNNNNGTG	1 cut(s)	458
PaeR7I	CTCGAG	1 cut(s)	2558
PagI	TCATGA	1 cut(s)	1203
PceI	AGGCCT	1 cut(s)	2195
PciSI	GCTCTTC	2 cut(s)	105, 369
PctI	GAATGC	1 cut(s)	1768
PdmI	GAANNNNTTC	3 cut(s)	114, 1254, 1406
PkrI	GCNGC	7 cut(s)	162, 1065, 1119, 1459, 2314, 2530, 2650
PleI	GAGTC	5 cut(s)	125, 235, 762, 900, 1831
PpsI	GAGTC	5 cut(s)	125, 235, 762, 900, 1831
Ppu21I	YACGTR	1 cut(s)	2428
PsiI	TTATAA	3 cut(s)	1367, 1724, 2243
Psp124BI	GAGCTC	1 cut(s)	1089
Psp6I	CCWGG	5 cut(s)	1175, 1623, 1651, 1870, 2208
PspFI	CCCAGC	1 cut(s)	1276
PspGI	CCWGG	5 cut(s)	1175, 1623, 1651, 1870, 2208
PspN4I	GGNNCC	7 cut(s)	155, 1036, 1650, 1704, 1869, 2399, 2640
PspPI	GGNCC	6 cut(s)	79, 153, 1326, 1627, 1867, 2009
PspXI	VCTCGAGB	1 cut(s)	2558
PstI	CTGCAG	1 cut(s)	2317
PsuI	RGATCY	1 cut(s)	1750
RsaI	GTAC	3 cut(s)	640, 1798, 1830
RsaNI	GTAC	3 cut(s)	639, 1797, 1829
RseI	CAYNNNNRTG	2 cut(s)	458, 1812
SacI	GAGCTC	1 cut(s)	1089
SapI	GCTCTTC	2 cut(s)	105, 369
SaqAI	TTAA	8 cut(s)	476, 540, 1047, 1811, 1848, 2142, 2387, 2417
SatI	GCNGC	7 cut(s)	161, 1064, 1118, 1458, 2313, 2529, 2649
Sau3AI	GATC	5 cut(s)	1750, 1981, 2232, 2474, 2715
Sau96I	GGNCC	6 cut(s)	79, 153, 1326, 1627, 1867, 2009
SchI	GAGTC	5 cut(s)	125, 236, 762, 900, 1832
ScrFI	CCNGG	6 cut(s)	157, 1177, 1625, 1653, 1872, 2210
SduI	GDGCHC	4 cut(s)	1089, 1215, 1651, 2673
SexAI	ACCWGGT	1 cut(s)	1651
SfaNI	GCATC	6 cut(s)	79, 1178, 1274, 1285, 1765, 2621
SfcI	CTRYAG	1 cut(s)	2313
Sfr274I	CTCGAG	1 cut(s)	2558
SfuI	TTCGAA	2 cut(s)	524, 1562
SgrAI	CRCCGGYG	1 cut(s)	2404
SinI	GGWCC	5 cut(s)	79, 153, 1326, 1867, 2009
SlaI	CTCGAG	1 cut(s)	2558
SmiMI	CAYNNNNRTG	2 cut(s)	458, 1812
SmlI	CTYRAG	2 cut(s)	611, 2558
SmoI	CTYRAG	2 cut(s)	611, 2558
SseBI	AGGCCT	1 cut(s)	2195
SsiI	CCGC	5 cut(s)	163, 545, 1457, 2402, 2723
SspMI	CTAG	5 cut(s)	426, 752, 2039, 2501, 2773
SstI	GAGCTC	1 cut(s)	1089
StuI	AGGCCT	1 cut(s)	2195
StyD4I	CCNGG	6 cut(s)	155, 1175, 1623, 1651, 1870, 2208
StyI	CCWWGG	7 cut(s)	763, 901, 1039, 1128, 1155, 1630, 2196
TaaI	ACNGT	9 cut(s)	126, 508, 638, 1330, 1363, 1483, 1552, 2109, 2741
TaiI	ACGT	2 cut(s)	352, 2430
TatI	WGTACW	3 cut(s)	638, 1796, 1828
TauI	GCSGC	1 cut(s)	1460
Tru1I	TTAA	8 cut(s)	476, 540, 1047, 1811, 1848, 2142, 2387, 2417
Tru9I	TTAA	8 cut(s)	476, 540, 1047, 1811, 1848, 2142, 2387, 2417
TscAI	CASTG	6 cut(s)	68, 460, 1220, 1345, 1919, 2054
TseFI	GTSAC	2 cut(s)	451, 569
TseI	GCWGC	6 cut(s)	160, 1063, 1117, 2312, 2528, 2648
Tsp45I	GTSAC	2 cut(s)	451, 569
TspGWI	ACGGA	4 cut(s)	398, 480, 2277, 2281
TspRI	CASTG	6 cut(s)	68, 460, 1220, 1345, 1919, 2054
VpaK11BI	GGWCC	5 cut(s)	79, 153, 1326, 1867, 2009
XapI	RAATTY	8 cut(s)	394, 947, 999, 1709, 1783, 2080, 2266, 2567
XceI	RCATGY	1 cut(s)	2312
XcmI	CCANNNNNNNNNTGG	1 cut(s)	670
XhoI	CTCGAG	1 cut(s)	2558
XmnI	GAANNNNTTC	3 cut(s)	114, 1254, 1406
XspI	CTAG	5 cut(s)	426, 752, 2039, 2501, 2773

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.