pycom12g23190

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007620
Physical Location & Seq
Forward (+)
23842598 .. 23844290
1693 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1098 bp
ATGGCTAGCTCATTGGCCATTTTTGGTGTGCTTATGATCATGGCGTTGGGAATGTTGAGTTATGAGGCTGAGGCTCGTGCCTTTTTTGTCTTTGGAGACTCACTGGTCGACAATGGCAACAACAACTACTTAGCCACCACCGCTCGTGCCGACTCTCCTCCTTACGGCATTGACTATCCCACCGGTCGACCCACCGGCCGTTTCTCCAATGGCTTGAACATTCCTGACTTCATCAGTTACGGCCAGCAACTTGGCGCAGAACCTACATTGCCATACTTGAGTCCAGAGCTCACAGGGCAAAAGCTACTAGTTGGTGCAAACTTTGCCTCTGCTGGCATTGGAATTCTCAATGACACTGGGATTCAGTTTATAAATATAATCAGAATGACAAGGCAATTTGAATACTTCCAACAATACCAGCAAAGGCTGTCTTCCCTAATCGGACCTCAACGTACCAAACAACTCGTAAATCAAGCACTCGTCCTCCTCACCGTCGGCGGCAACGATATTGTCAACAACTACTACCTGGTGCCTTTCTCCGCAAGATCTCGCCAGTTTGCTCTGCCAGACTATGTCAGATACCTCATTTCCGAGCTCCGAAAACTTCTCTTGAAAATGTATGATCTTGGAGCACGAAGGGTTTTGGTGACGGGGACAGGACCACTAGGCTGTGTGCCGGCCGAATTGGCCATGAGAAGCAGGAACGGAGAATGCTCAGCGGAGCTGCAACAAGCTGCGTCTCTGTACAACCCCCAACTCATTCAGATGCTGAGATCAGTCAACAGCCAACTCGGTTCGGATGTCTTCATTGCTGCAAACACTCAGCAGACCCACAATGACTTCGTCAGCAACCCTAAAGCTTTTGGATTTTCTACGTCAAAGATTGCATGCTGTGGGCAAGGACCTTACAATGGCTTGGGGCTGTGCACGGTGGCCTCAAACTTGTGCCCGAACCGCAACCAATACGCTTTCTGGGATGCATTTCATCCATCTGAAAAGGCGAACAAAGTGATCGTTCAGAATATTTTGAAAGGGGATGAAAAGTACATGTACCCCATGAACCTCAGCACCATCTTGGCTTTGGATTCCAGGACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

366

Amino Acids

40.13

Weight (kDa)

8.64

Isoelectric Point (pI)

39.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 29 - 340 1.1e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 371
AasI GACNNNNNNGTC 1 cut(s) 104
AccB1I GGYRCC 1 cut(s) 529
AccBSI CCGCTC 1 cut(s) 143
AccI GTMKAC 2 cut(s) 108, 187
AciI CCGC 5 cut(s) 141, 498, 540, 719, 955
AcoI YGGCCR 5 cut(s) 15, 196, 241, 678, 687
AcsI RAATTY 1 cut(s) 342
AfaI GTAC 4 cut(s) 454, 746, 1046, 1052
AfiI CCNNNNNNNGG 2 cut(s) 164, 911
AflIII ACRYGT 1 cut(s) 1047
AgeI ACCGGT 1 cut(s) 182
AgsI TTSAA 4 cut(s) 217, 401, 613, 1030
AhlI ACTAGT 1 cut(s) 307
AjnI CCWGG 2 cut(s) 525, 1088
AluBI AGCT 7 cut(s) 9, 289, 304, 595, 724, 734, 860
AluI AGCT 7 cut(s) 9, 289, 304, 595, 724, 734, 860
Alw21I GWGCWC 4 cut(s) 291, 597, 634, 929
Alw26I GTCTC 2 cut(s) 90, 744
Alw44I GTGCAC 1 cut(s) 925
AlwNI CAGNNNCTG 1 cut(s) 769
AoxI GGCC 6 cut(s) 15, 196, 241, 678, 687, 933
ApaLI GTGCAC 1 cut(s) 925
ApeKI GCWGC 3 cut(s) 724, 734, 812
ApoI RAATTY 1 cut(s) 342
AsiGI ACCGGT 1 cut(s) 182
AspLEI GCGC 1 cut(s) 257
AspS9I GGNCC 4 cut(s) 443, 659, 902, 1092
AsuHPI GGTGA 2 cut(s) 481, 658
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 4 cut(s) 443, 659, 902, 1092
BaeGI GKGCMC 2 cut(s) 929, 950
BalI TGGCCA 2 cut(s) 17, 689
BanI GGYRCC 1 cut(s) 529
BanII GRGCYC 2 cut(s) 291, 597
BauI CACGAG 2 cut(s) 75, 144
BbsI GAAGAC 2 cut(s) 423, 796
Bbv12I GWGCWC 4 cut(s) 291, 597, 634, 929
BbvCI CCTCAGC 2 cut(s) 69, 1064
BbvI GCAGC 3 cut(s) 711, 721, 799
BccI CCATC 2 cut(s) 997, 1079
BceAI ACGGC 3 cut(s) 181, 183, 256
BciT130I CCWGG 2 cut(s) 527, 1090
BclI TGATCA 1 cut(s) 36
BcoDI GTCTC 2 cut(s) 90, 744
BcuI ACTAGT 1 cut(s) 307
BfaI CTAG 3 cut(s) 6, 308, 665
BglI GCCNNNNNGGC 1 cut(s) 686
BglII AGATCT 1 cut(s) 545
BisI GCNGC 4 cut(s) 499, 725, 735, 813
BlpI GCTNAGC 1 cut(s) 715
BlsI GCNGC 4 cut(s) 500, 726, 736, 814
Bme1390I CCNGG 2 cut(s) 527, 1090
Bme18I GGWCC 4 cut(s) 443, 659, 902, 1092
BmgT120I GGNCC 4 cut(s) 443, 659, 902, 1092
BmiI GGNNCC 1 cut(s) 531
BmrFI CCNGG 2 cut(s) 527, 1090
BmrI ACTGGG 1 cut(s) 366
BmsI GCATC 2 cut(s) 756, 967
BmtI GCTAGC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 366
BpiI GAAGAC 2 cut(s) 423, 796
Bpu10I CCTNAGC 2 cut(s) 69, 1064
Bpu1102I GCTNAGC 1 cut(s) 715
BpuEI CTTGAG 1 cut(s) 298
BsaWI WCCGGW 1 cut(s) 182
BsaXI ACNNNNNCTCC 2 cut(s) 468, 498
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 911
Bse118I RCCGGY 3 cut(s) 182, 194, 676
Bse1I ACTGG 3 cut(s) 108, 361, 553
Bse3DI GCAATG 2 cut(s) 266, 807
BseBI CCWGG 2 cut(s) 527, 1090
BseGI GGATG 4 cut(s) 805, 982, 985, 1042
BseLI CCNNNNNNNGG 2 cut(s) 164, 911
BseMI GCAATG 2 cut(s) 266, 807
BseMII CTCAG 5 cut(s) 60, 729, 761, 836, 1078
BseNI ACTGG 3 cut(s) 108, 361, 553
BseRI GAGGAG 2 cut(s) 147, 476
BseSI GKGCMC 2 cut(s) 929, 950
BseX3I CGGCCG 2 cut(s) 196, 678
BseXI GCAGC 3 cut(s) 711, 721, 799
Bsh1285I CGRYCG 3 cut(s) 187, 199, 681
BshFI GGCC 6 cut(s) 17, 198, 243, 680, 689, 935
BshNI GGYRCC 1 cut(s) 529
BshTI ACCGGT 1 cut(s) 182
BsiEI CGRYCG 3 cut(s) 187, 199, 681
BsiHKAI GWGCWC 4 cut(s) 291, 597, 634, 929
BsiSI CCGG 3 cut(s) 183, 195, 677
BslFI GGGAC 1 cut(s) 667
BslI CCNNNNNNNGG 2 cut(s) 164, 911
BsmAI GTCTC 2 cut(s) 90, 744
BsmBI CGTCTC 1 cut(s) 744
BsmFI GGGAC 1 cut(s) 667
BsmI GAATGC 1 cut(s) 716
BsnI GGCC 6 cut(s) 17, 198, 243, 680, 689, 935
Bsp1286I GDGCHC 5 cut(s) 291, 597, 634, 929, 950
Bsp1407I TGTACA 1 cut(s) 744
Bsp143I GATC 5 cut(s) 36, 545, 622, 773, 1011
Bsp1720I GCTNAGC 1 cut(s) 715
BspACI CCGC 5 cut(s) 141, 498, 540, 719, 955
BspANI GGCC 6 cut(s) 17, 198, 243, 680, 689, 935
BspCNI CTCAG 5 cut(s) 61, 728, 762, 835, 1077
BspLI GGNNCC 1 cut(s) 531
BspOI GCTAGC 1 cut(s) 9
BspT107I GGYRCC 1 cut(s) 529
BsrBI CCGCTC 1 cut(s) 143
BsrDI GCAATG 2 cut(s) 266, 807
BsrFI RCCGGY 3 cut(s) 182, 194, 676
BsrGI TGTACA 1 cut(s) 744
BsrI ACTGG 3 cut(s) 108, 361, 553
BssAI RCCGGY 3 cut(s) 182, 194, 676
BssMI GATC 5 cut(s) 36, 545, 622, 773, 1011
BssSI CACGAG 2 cut(s) 75, 144
Bst2BI CACGAG 2 cut(s) 75, 144
Bst2UI CCWGG 2 cut(s) 527, 1090
Bst4CI ACNGT 2 cut(s) 493, 931
BstAPI GCANNNNNTGC 1 cut(s) 323
BstAUI TGTACA 1 cut(s) 744
BstC8I GCNNGC 5 cut(s) 7, 245, 334, 678, 889
BstDEI CTNAG 6 cut(s) 69, 130, 715, 770, 822, 1064
BstF5I GGATG 4 cut(s) 805, 982, 985, 1042
BstHHI GCGC 1 cut(s) 257
BstKTI GATC 5 cut(s) 39, 548, 625, 776, 1014
BstMAI GTCTC 2 cut(s) 90, 744
BstMBI GATC 5 cut(s) 36, 545, 622, 773, 1011
BstMCI CGRYCG 3 cut(s) 187, 199, 681
BstMWI GCNNNNNNNGC 5 cut(s) 140, 295, 323, 686, 954
BstNI CCWGG 2 cut(s) 527, 1090
BstNSI RCATGY 2 cut(s) 891, 1051
BstSCI CCNGG 2 cut(s) 525, 1088
BstSLI GKGCMC 2 cut(s) 929, 950
BstV1I GCAGC 3 cut(s) 711, 721, 799
BstV2I GAAGAC 2 cut(s) 423, 796
BstX2I RGATCY 1 cut(s) 545
BstXI CCANNNNNNTGG 1 cut(s) 251
BstYI RGATCY 1 cut(s) 545
BstZI CGGCCG 2 cut(s) 196, 678
BsuRI GGCC 6 cut(s) 17, 198, 243, 680, 689, 935
BtsCI GGATG 4 cut(s) 805, 982, 985, 1042
BtsIMutI CAGTG 2 cut(s) 101, 354
Cac8I GCNNGC 5 cut(s) 7, 245, 334, 678, 889
CaiI CAGNNNCTG 1 cut(s) 769
CfoI GCGC 1 cut(s) 257
Cfr10I RCCGGY 3 cut(s) 182, 194, 676
Cfr13I GGNCC 4 cut(s) 443, 659, 902, 1092
CseI GACGC 1 cut(s) 726
CsiI ACCWGGT 1 cut(s) 525
Csp6I GTAC 4 cut(s) 453, 745, 1045, 1051
CspAI ACCGGT 1 cut(s) 182
CviAII CATG 5 cut(s) 40, 691, 888, 1048, 1057
CviQI GTAC 4 cut(s) 453, 745, 1045, 1051
DdeI CTNAG 6 cut(s) 69, 130, 715, 770, 822, 1064
DpnI GATC 5 cut(s) 38, 547, 624, 775, 1013
DpnII GATC 5 cut(s) 36, 545, 622, 773, 1011
DrdI GACNNNNNNGTC 1 cut(s) 104
DseDI GACNNNNNNGTC 1 cut(s) 104
EaeI YGGCCR 5 cut(s) 15, 196, 241, 678, 687
EagI CGGCCG 2 cut(s) 196, 678
Ecl136II GAGCTC 2 cut(s) 289, 595
EclXI CGGCCG 2 cut(s) 196, 678
Eco24I GRGCYC 2 cut(s) 291, 597
Eco47I GGWCC 4 cut(s) 443, 659, 902, 1092
Eco52I CGGCCG 2 cut(s) 196, 678
Eco53kI GAGCTC 2 cut(s) 289, 595
EcoICRI GAGCTC 2 cut(s) 289, 595
EcoO109I RGGNCCY 2 cut(s) 902, 1092
EcoRI GAATTC 1 cut(s) 342
EcoRII CCWGG 2 cut(s) 525, 1088
EcoT22I ATGCAT 1 cut(s) 982
EcoT38I GRGCYC 2 cut(s) 291, 597
Esp3I CGTCTC 1 cut(s) 744
FaeI CATG 5 cut(s) 43, 694, 891, 1051, 1060
FalI AAGNNNNNCTT 2 cut(s) 415, 447
FaqI GGGAC 1 cut(s) 667
FatI CATG 5 cut(s) 39, 690, 887, 1047, 1056
FbaI TGATCA 1 cut(s) 36
FblI GTMKAC 2 cut(s) 108, 187
Fnu4HI GCNGC 4 cut(s) 499, 725, 735, 813
FokI GGATG 4 cut(s) 812, 972, 989, 1049
FriOI GRGCYC 2 cut(s) 291, 597
Fsp4HI GCNGC 4 cut(s) 499, 725, 735, 813
FspBI CTAG 3 cut(s) 6, 308, 665
GlaI GCGC 1 cut(s) 256
GluI GCNGC 4 cut(s) 499, 725, 735, 813
HaeIII GGCC 6 cut(s) 17, 198, 243, 680, 689, 935
HapII CCGG 3 cut(s) 183, 195, 677
HgaI GACGC 1 cut(s) 726
HhaI GCGC 1 cut(s) 257
Hin1II CATG 5 cut(s) 43, 694, 891, 1051, 1060
Hin6I GCGC 1 cut(s) 255
HinP1I GCGC 1 cut(s) 255
HincII GTYRAC 4 cut(s) 109, 188, 514, 781
HindII GTYRAC 4 cut(s) 109, 188, 514, 781
HindIII AAGCTT 1 cut(s) 858
HinfI GANTC 5 cut(s) 98, 152, 280, 361, 1085
HpaII CCGG 3 cut(s) 183, 195, 677
HphI GGTGA 2 cut(s) 481, 658
Hpy166II GTNNAC 5 cut(s) 109, 188, 514, 781, 927
Hpy188I TCNGA 9 cut(s) 383, 443, 578, 592, 599, 765, 799, 994, 1020
Hpy188III TCNNGA 3 cut(s) 224, 284, 610
Hpy8I GTNNAC 5 cut(s) 109, 188, 514, 781, 927
Hpy99I CGWCG 1 cut(s) 497
HpyAV CCTTC 1 cut(s) 630
HpyCH4III ACNGT 2 cut(s) 493, 931
HpyCH4IV ACGT 2 cut(s) 451, 875
HpyCH4V TGCA 6 cut(s) 317, 727, 815, 887, 927, 980
HpyF10VI GCNNNNNNNGC 5 cut(s) 140, 295, 323, 686, 954
HpyF3I CTNAG 6 cut(s) 69, 130, 715, 770, 822, 1064
HpySE526I ACGT 2 cut(s) 451, 875
Hsp92II CATG 5 cut(s) 43, 694, 891, 1051, 1060
HspAI GCGC 1 cut(s) 255
KroI GCCGGC 1 cut(s) 676
KroNI GCCGGC 1 cut(s) 678
Ksp22I TGATCA 1 cut(s) 36
Kzo9I GATC 5 cut(s) 36, 545, 622, 773, 1011
LmnI GCTCC 3 cut(s) 600, 629, 721
Lsp1109I GCAGC 3 cut(s) 711, 721, 799
LweI GCATC 2 cut(s) 756, 967
MabI ACCWGGT 1 cut(s) 525
MaeI CTAG 3 cut(s) 6, 308, 665
MaeII ACGT 2 cut(s) 451, 875
MaeIII GTNAC 2 cut(s) 236, 646
MalI GATC 5 cut(s) 38, 547, 624, 775, 1013
MbiI CCGCTC 1 cut(s) 143
MboI GATC 5 cut(s) 36, 545, 622, 773, 1011
MboII GAAGA 2 cut(s) 423, 796
MflI RGATCY 1 cut(s) 545
MhlI GDGCHC 5 cut(s) 291, 597, 634, 929, 950
MlsI TGGCCA 2 cut(s) 17, 689
MluCI AATT 3 cut(s) 342, 395, 683
MluNI TGGCCA 2 cut(s) 17, 689
MlyI GAGTC 3 cut(s) 92, 146, 289
MmeI TCCRAC 1 cut(s) 433
Mox20I TGGCCA 2 cut(s) 17, 689
Mph1103I ATGCAT 1 cut(s) 982
MroNI GCCGGC 1 cut(s) 676
MscI TGGCCA 2 cut(s) 17, 689
MslI CAYNNNNRTG 1 cut(s) 764
Msp20I TGGCCA 2 cut(s) 17, 689
MspA1I CMGCKG 1 cut(s) 719
MspI CCGG 3 cut(s) 183, 195, 677
MspR9I CCNGG 2 cut(s) 527, 1090
Mva1269I GAATGC 1 cut(s) 716
MvaI CCWGG 2 cut(s) 527, 1090
MwoI GCNNNNNNNGC 5 cut(s) 140, 295, 323, 686, 954
NaeI GCCGGC 1 cut(s) 678
NdeII GATC 5 cut(s) 36, 545, 622, 773, 1011
NgoMIV GCCGGC 1 cut(s) 676
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 5 cut(s) 43, 694, 891, 1051, 1060
NlaIV GGNNCC 1 cut(s) 531
NmuCI GTSAC 1 cut(s) 646
NsiI ATGCAT 1 cut(s) 982
NspI RCATGY 2 cut(s) 891, 1051
PaeI GCATGC 1 cut(s) 891
PciI ACATGT 1 cut(s) 1047
PcsI WCGNNNNNNNCGW 1 cut(s) 501
PctI GAATGC 1 cut(s) 716
PdiI GCCGGC 1 cut(s) 678
PfeI GAWTC 2 cut(s) 361, 1085
PflFI GACNNNGTC 2 cut(s) 572, 842
PfoI TCCNGGA 1 cut(s) 1088
PinAI ACCGGT 1 cut(s) 182
PkrI GCNGC 4 cut(s) 500, 726, 736, 814
PleI GAGTC 3 cut(s) 92, 146, 288
PpsI GAGTC 3 cut(s) 92, 146, 288
PpuMI RGGWCCY 2 cut(s) 902, 1092
PscI ACATGT 1 cut(s) 1047
PsiI TTATAA 1 cut(s) 371
Psp124BI GAGCTC 2 cut(s) 291, 597
Psp5II RGGWCCY 2 cut(s) 902, 1092
Psp6I CCWGG 2 cut(s) 525, 1088
PspGI CCWGG 2 cut(s) 525, 1088
PspN4I GGNNCC 1 cut(s) 531
PspPI GGNCC 4 cut(s) 443, 659, 902, 1092
PspPPI RGGWCCY 2 cut(s) 902, 1092
PstNI CAGNNNCTG 1 cut(s) 769
PsuI RGATCY 1 cut(s) 545
PsyI GACNNNGTC 2 cut(s) 572, 842
RsaI GTAC 4 cut(s) 454, 746, 1046, 1052
RsaNI GTAC 4 cut(s) 453, 745, 1045, 1051
RseI CAYNNNNRTG 1 cut(s) 764
SacI GAGCTC 2 cut(s) 291, 597
SalI GTCGAC 2 cut(s) 107, 186
SatI GCNGC 4 cut(s) 499, 725, 735, 813
Sau3AI GATC 5 cut(s) 36, 545, 622, 773, 1011
Sau96I GGNCC 4 cut(s) 443, 659, 902, 1092
SchI GAGTC 3 cut(s) 92, 146, 289
ScrFI CCNGG 2 cut(s) 527, 1090
SduI GDGCHC 5 cut(s) 291, 597, 634, 929, 950
SexAI ACCWGGT 1 cut(s) 525
SfaNI GCATC 2 cut(s) 756, 967
SfiI GGCCNNNNNGGCC 1 cut(s) 686
SinI GGWCC 4 cut(s) 443, 659, 902, 1092
SmiMI CAYNNNNRTG 1 cut(s) 764
SmlI CTYRAG 1 cut(s) 277
SmoI CTYRAG 1 cut(s) 277
SpeI ACTAGT 1 cut(s) 307
SphI GCATGC 1 cut(s) 891
Sse9I AATT 3 cut(s) 342, 395, 683
SsiI CCGC 5 cut(s) 141, 498, 540, 719, 955
SspI AATATT 1 cut(s) 1024
SspMI CTAG 3 cut(s) 6, 308, 665
SstI GAGCTC 2 cut(s) 291, 597
StyD4I CCNGG 2 cut(s) 525, 1088
TaaI ACNGT 2 cut(s) 493, 931
TaiI ACGT 2 cut(s) 454, 878
TaqI TCGA 2 cut(s) 108, 187
TasI AATT 3 cut(s) 342, 395, 683
TatI WGTACW 2 cut(s) 744, 1044
TauI GCSGC 1 cut(s) 501
TfiI GAWTC 2 cut(s) 361, 1085
TscAI CASTG 2 cut(s) 108, 361
TseFI GTSAC 1 cut(s) 646
TseI GCWGC 3 cut(s) 724, 734, 812
Tsp45I GTSAC 1 cut(s) 646
TspDTI ATGAA 5 cut(s) 220, 796, 974, 1053, 1073
TspGWI ACGGA 1 cut(s) 720
TspRI CASTG 2 cut(s) 108, 361
Tth111I GACNNNGTC 2 cut(s) 572, 842
VneI GTGCAC 1 cut(s) 925
VpaK11BI GGWCC 4 cut(s) 443, 659, 902, 1092
XapI RAATTY 1 cut(s) 342
XceI RCATGY 2 cut(s) 891, 1051
XcmI CCANNNNNNNNNTGG 1 cut(s) 1078
XmiI GTMKAC 2 cut(s) 108, 187
XspI CTAG 3 cut(s) 6, 308, 665
Zsp2I ATGCAT 1 cut(s) 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.