pycom13g00160

recombinational repair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003214
Physical Location & Seq
Forward (+)
106102 .. 108830
2729 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1374 bp
ATGTGTATTACTTATGCTGTTAGCAAAGACCTGCTTCTGGGAATTGATACCAATAGCTCTTGGATGAGTGCAGTCCTATGTAATGCTACTAATGAAGGGGGGATGCACATGTTTGGTCATGCGGATCATCTTTATGACTTCTTTGATTCAACTTGGAGATATGGATGGATTGGCAGTTTAATCTCAAAAAACCGCACGGATGTGATTGGCTTACCTGTGTGGAACTCACTTTCTAAGTTGGTTGAAGAAAGGGAAACTCAATTTTCAACCTTCATGGATCAAAGGGAATTTATAGATATTAGTTCATCGGATAGTGAATTAGAGATAGAAGAAAGGGAATATGGCAACAGCAGAGTTCTTCCAAGTTCGCTTTCTGGACCTAGCTCAAATCCAAGGAGTAGAGATTATTCTGGGCAGTTTAGAAAGATGCCTTCTCCTAGAAGAGCATATGCTTCTAATGGCAACTCTCCTAATGTTAATCAGCACACACAGGTGAAACAGAATTTTAATCCCAGTTCAAGTGATGATATAAGAACCTCAAATCGACATGCTGCTCGAGCACATAATGATAATGTGGAACGGCCTCAAAGTAATCATTCACAAAGTTCTAATATTTCTCTTAAGGACTACGAGGCTCAGAAGAGGACCCTTCCACAATCTATGCAGCTATCGGGACCAAGTACTTATGCAAGCAATGGCAAGGGCCTTATGAGAGACTATTCTAGTAGGGGCTATGACAACGAATTTATTAGGTCTGAAAGCAGTGGGAGTAGGGGCCAGCCTCCCTCTTTCATGCATGGAAAGTCATTTTCTGCTTCACAGTTTGCGAGTTCAAGTGACCCTGCATACCATTCTGGGACAGGTGACGAAAGGGTTGCTGGCAGTGATGAGAGACTGATATATCAAGCAGCATTAGAGCAGAGATCGATCAATCAACCAAAAGTTGAAGCTACTTTACCTGATGGTCTTCTGTCGGTCCCTCTTCTACGACATCAGAAAATTGCTCTGGCATGGATGCTTCAAAAGGAAACTAGAAGTCTGCATTGTCTGGGTGGAATCTTAGCAGATGATCAGGGCCTTGGTAAGACCATTTCAATGATTGCCCTTATACAAATGCAGAAGTATTTGGATTCAAAGTCAAAATCTAGAGATTTAGGCAATCAGAAAACTGAGGCTTTAAATTTGGATGACGATGAGGACAATCCCAACGGTGGTTTGGATAAATTGAATAAAACTGAGGCACCTGATCATCTTCGATCAACTCCAGAAGTTAGTACATCTTCAAAAATGATTGGATCGATCAATCAGAAAGCAGAGGCCAGCAGCAGGTACATTGGTTGTGTGTCCAGCAAGTGTTCTTCGACAGTGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000278 GO:0000724 GO:0000725 GO:0000785 GO:0000790 GO:0003674 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006304 GO:0006305 GO:0006306 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007154 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008270 GO:0009058 GO:0009059 GO:0009292 GO:0009294 GO:0009605 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0009991 GO:0010468 GO:0010605 GO:0010629 GO:0016043 GO:0016070 GO:0016458 GO:0016462 GO:0016567 GO:0016740 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019538 GO:0019787 GO:0022402 GO:0030162 GO:0031047 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031668 GO:0031974 GO:0031981 GO:0032259 GO:0032268 GO:0032269 GO:0032392 GO:0032434 GO:0032435 GO:0032446 GO:0032508 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0042176 GO:0042177 GO:0042262 GO:0042623 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0044764 GO:0044786 GO:0045003 GO:0045005 GO:0045861 GO:0046483 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051276 GO:0051704 GO:0051716 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0070013 GO:0070035 GO:0070647 GO:0071103 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0140083 GO:0140096 GO:0140097 GO:1901360 GO:1901564 GO:1901576 GO:1901799 GO:1902298 GO:1902969 GO:1903047 GO:1903050 GO:1903051 GO:1903362 GO:1903363 GO:1990505 GO:2000058 GO:2000059
KEGG Pathways
Metabolic & Signaling
ko04918 map04918
Pfam Domains
Protein Families
Helicase_C SNF2_N zf-C3HC4 zf-C3HC4_2 zf-C3HC4_3

Protein Analysis

458

Amino Acids

50.51

Weight (kDa)

6.33

Isoelectric Point (pI)

53.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 331 - 379 1.4e-09 SNF2-related domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 39, 1317
AccB1I GGYRCC 1 cut(s) 1240
AciI CCGC 2 cut(s) 122, 193
AclWI GGATC 3 cut(s) 132, 285, 1303
AcsI RAATTY 4 cut(s) 287, 502, 743, 1180
AfaI GTAC 3 cut(s) 682, 1276, 1331
AfiI CCNNNNNNNGG 3 cut(s) 37, 1211, 1326
AflII CTTAAG 1 cut(s) 620
AflIII ACRYGT 1 cut(s) 108
AjuI GAANNNNNNNTTGG 2 cut(s) 355, 387
AleI CACNNNNGTG 2 cut(s) 200, 491
AluBI AGCT 4 cut(s) 57, 384, 667, 950
AluI AGCT 4 cut(s) 57, 384, 667, 950
Alw21I GWGCWC 1 cut(s) 562
Alw26I GTCTC 2 cut(s) 708, 886
AlwI GGATC 3 cut(s) 132, 285, 1303
Ama87I CYCGRG 1 cut(s) 555
AoxI GGCC 5 cut(s) 581, 703, 775, 1075, 1317
ApeKI GCWGC 4 cut(s) 551, 664, 908, 1323
ApoI RAATTY 4 cut(s) 287, 502, 743, 1180
AspS9I GGNCC 7 cut(s) 377, 645, 674, 703, 775, 976, 1075
AsuHPI GGTGA 2 cut(s) 505, 875
AvaI CYCGRG 1 cut(s) 555
AvaII GGWCC 4 cut(s) 377, 645, 674, 976
BaeI ACNNNNGTAYC 2 cut(s) 1321, 1354
BanI GGYRCC 1 cut(s) 1240
BarI GAAGNNNNNNTAC 2 cut(s) 939, 971
BbsI GAAGAC 1 cut(s) 959
Bbv12I GWGCWC 1 cut(s) 562
BbvI GCAGC 4 cut(s) 538, 676, 920, 1335
BccI CCATC 2 cut(s) 159, 956
BceAI ACGGC 1 cut(s) 596
BcgI CGANNNNNNTGC 2 cut(s) 857, 891
BclI TGATCA 2 cut(s) 1069, 1246
BcoDI GTCTC 2 cut(s) 708, 886
BfaI CTAG 6 cut(s) 381, 438, 723, 1032, 1146, 1372
BfrI CTTAAG 1 cut(s) 620
BfuAI ACCTGC 2 cut(s) 39, 1317
BisI GCNGC 4 cut(s) 552, 665, 909, 1324
BlsI GCNGC 4 cut(s) 553, 666, 910, 1325
BmcAI AGTACT 1 cut(s) 682
Bme18I GGWCC 4 cut(s) 377, 645, 674, 976
BmeT110I CYCGRG 1 cut(s) 555
BmgT120I GGNCC 7 cut(s) 377, 645, 674, 703, 775, 976, 1075
BmiI GGNNCC 5 cut(s) 647, 675, 776, 978, 1242
BmrI ACTGGG 1 cut(s) 507
BmsI GCATC 3 cut(s) 93, 417, 1005
BmuI ACTGGG 1 cut(s) 507
BpiI GAAGAC 1 cut(s) 959
BpmI CTGGAG 1 cut(s) 1248
Bsa29I ATCGAT 2 cut(s) 926, 1298
BsaJI CCNNGG 2 cut(s) 392, 1078
Bsc4I CCNNNNNNNGG 3 cut(s) 37, 1211, 1326
Bse1I ACTGG 1 cut(s) 513
Bse3DI GCAATG 1 cut(s) 700
BseCI ATCGAT 2 cut(s) 926, 1298
BseDI CCNNGG 2 cut(s) 392, 1078
BseGI GGATG 6 cut(s) 69, 108, 170, 205, 1020, 1192
BseLI CCNNNNNNNGG 3 cut(s) 37, 1211, 1326
BseMI GCAATG 1 cut(s) 700
BseMII CTCAG 3 cut(s) 650, 1161, 1227
BseNI ACTGG 1 cut(s) 513
BseXI GCAGC 4 cut(s) 538, 676, 920, 1335
BsgI GTGCAG 1 cut(s) 90
BshFI GGCC 5 cut(s) 583, 705, 777, 1077, 1319
BshNI GGYRCC 1 cut(s) 1240
BshVI ATCGAT 2 cut(s) 926, 1298
BsiHKAI GWGCWC 1 cut(s) 562
BsiHKCI CYCGRG 1 cut(s) 555
BslFI GGGAC 3 cut(s) 687, 871, 962
BslI CCNNNNNNNGG 3 cut(s) 37, 1211, 1326
BsmAI GTCTC 2 cut(s) 708, 886
BsmFI GGGAC 3 cut(s) 687, 871, 962
BsnI GGCC 5 cut(s) 583, 705, 777, 1077, 1319
BsoBI CYCGRG 1 cut(s) 555
Bsp1286I GDGCHC 1 cut(s) 562
Bsp143I GATC 9 cut(s) 124, 277, 923, 927, 1069, 1246, 1256, 1295, 1299
BspACI CCGC 2 cut(s) 122, 193
BspANI GGCC 5 cut(s) 583, 705, 777, 1077, 1319
BspCNI CTCAG 3 cut(s) 649, 1162, 1228
BspDI ATCGAT 2 cut(s) 926, 1298
BspLI GGNNCC 5 cut(s) 647, 675, 776, 978, 1242
BspMI ACCTGC 2 cut(s) 39, 1317
BspPI GGATC 3 cut(s) 132, 285, 1303
BspQI GCTCTTC 1 cut(s) 436
BspT107I GGYRCC 1 cut(s) 1240
BspTI CTTAAG 1 cut(s) 620
BsrDI GCAATG 1 cut(s) 700
BsrI ACTGG 1 cut(s) 513
BssECI CCNNGG 2 cut(s) 392, 1078
BssMI GATC 9 cut(s) 124, 277, 923, 927, 1069, 1246, 1256, 1295, 1299
BssT1I CCWWGG 2 cut(s) 392, 1078
Bst4CI ACNGT 3 cut(s) 822, 1211, 1366
Bst6I CTCTTC 3 cut(s) 436, 635, 987
BstAFI CTTAAG 1 cut(s) 620
BstC8I GCNNGC 4 cut(s) 691, 779, 880, 1321
BstDEI CTNAG 5 cut(s) 234, 636, 1060, 1170, 1236
BstF5I GGATG 6 cut(s) 69, 108, 170, 205, 1020, 1192
BstKTI GATC 9 cut(s) 127, 280, 926, 930, 1072, 1249, 1259, 1298, 1302
BstMAI GTCTC 2 cut(s) 708, 886
BstMBI GATC 9 cut(s) 124, 277, 923, 927, 1069, 1246, 1256, 1295, 1299
BstMWI GCNNNNNNNGC 1 cut(s) 557
BstNSI RCATGY 2 cut(s) 112, 551
BstV1I GCAGC 4 cut(s) 538, 676, 920, 1335
BstV2I GAAGAC 1 cut(s) 959
Bsu15I ATCGAT 2 cut(s) 926, 1298
BsuRI GGCC 5 cut(s) 583, 705, 777, 1077, 1319
BsuTUI ATCGAT 2 cut(s) 926, 1298
BtsCI GGATG 6 cut(s) 69, 108, 170, 205, 1020, 1192
BtsI GCAGTG 2 cut(s) 769, 889
BtsIMutI CAGTG 3 cut(s) 769, 889, 1371
BveI ACCTGC 2 cut(s) 39, 1317
Cac8I GCNNGC 4 cut(s) 691, 779, 880, 1321
Cfr13I GGNCC 7 cut(s) 377, 645, 674, 703, 775, 976, 1075
ClaI ATCGAT 2 cut(s) 926, 1298
Csp6I GTAC 3 cut(s) 681, 1275, 1330
CviAII CATG 7 cut(s) 109, 119, 274, 548, 793, 797, 1011
CviQI GTAC 3 cut(s) 681, 1275, 1330
DdeI CTNAG 5 cut(s) 234, 636, 1060, 1170, 1236
DpnI GATC 9 cut(s) 126, 279, 925, 929, 1071, 1248, 1258, 1297, 1301
DpnII GATC 9 cut(s) 124, 277, 923, 927, 1069, 1246, 1256, 1295, 1299
DraI TTTAAA 1 cut(s) 1179
Eam1104I CTCTTC 3 cut(s) 436, 635, 987
EarI CTCTTC 3 cut(s) 436, 635, 987
Eco130I CCWWGG 2 cut(s) 392, 1078
Eco47I GGWCC 4 cut(s) 377, 645, 674, 976
Eco88I CYCGRG 1 cut(s) 555
EcoO109I RGGNCCY 3 cut(s) 645, 703, 1075
EcoT14I CCWWGG 2 cut(s) 392, 1078
EcoT22I ATGCAT 1 cut(s) 798
ErhI CCWWGG 2 cut(s) 392, 1078
FaeI CATG 7 cut(s) 112, 122, 277, 551, 796, 800, 1014
FalI AAGNNNNNCTT 2 cut(s) 18, 50
FaqI GGGAC 3 cut(s) 687, 871, 962
FatI CATG 7 cut(s) 108, 118, 273, 547, 792, 796, 1010
FauNDI CATATG 1 cut(s) 448
FbaI TGATCA 2 cut(s) 1069, 1246
Fnu4HI GCNGC 4 cut(s) 552, 665, 909, 1324
FokI GGATG 6 cut(s) 76, 115, 177, 212, 1027, 1199
Fsp4HI GCNGC 4 cut(s) 552, 665, 909, 1324
FspBI CTAG 6 cut(s) 381, 438, 723, 1032, 1146, 1372
GluI GCNGC 4 cut(s) 552, 665, 909, 1324
GsuI CTGGAG 1 cut(s) 1248
HaeIII GGCC 5 cut(s) 583, 705, 777, 1077, 1319
Hin1II CATG 7 cut(s) 112, 122, 277, 551, 796, 800, 1014
HinfI GANTC 3 cut(s) 146, 1056, 1130
HphI GGTGA 2 cut(s) 505, 875
Hpy188I TCNGA 6 cut(s) 310, 639, 757, 996, 1164, 1308
Hpy188III TCNNGA 4 cut(s) 375, 672, 1146, 1265
HpyAV CCTTC 4 cut(s) 89, 280, 441, 659
HpyCH4III ACNGT 3 cut(s) 822, 1211, 1366
HpyCH4V TGCA 8 cut(s) 71, 106, 664, 689, 796, 845, 1042, 1117
HpyF10VI GCNNNNNNNGC 1 cut(s) 557
HpyF3I CTNAG 5 cut(s) 234, 636, 1060, 1170, 1236
Hsp92II CATG 7 cut(s) 112, 122, 277, 551, 796, 800, 1014
Ksp22I TGATCA 2 cut(s) 1069, 1246
Kzo9I GATC 9 cut(s) 124, 277, 923, 927, 1069, 1246, 1256, 1295, 1299
LguI GCTCTTC 1 cut(s) 436
Lsp1109I GCAGC 4 cut(s) 538, 676, 920, 1335
LweI GCATC 3 cut(s) 93, 417, 1005
MaeI CTAG 6 cut(s) 381, 438, 723, 1032, 1146, 1372
MaeIII GTNAC 2 cut(s) 836, 863
MalI GATC 9 cut(s) 126, 279, 925, 929, 1071, 1248, 1258, 1297, 1301
MboI GATC 9 cut(s) 124, 277, 923, 927, 1069, 1246, 1256, 1295, 1299
MhlI GDGCHC 1 cut(s) 562
MluCI AATT 9 cut(s) 42, 260, 287, 317, 502, 743, 999, 1180, 1223
Mph1103I ATGCAT 1 cut(s) 798
MseI TTAA 5 cut(s) 179, 477, 507, 621, 1178
MslI CAYNNNNRTG 4 cut(s) 132, 200, 491, 1094
MspCI CTTAAG 1 cut(s) 620
MwoI GCNNNNNNNGC 1 cut(s) 557
NdeI CATATG 1 cut(s) 448
NdeII GATC 9 cut(s) 124, 277, 923, 927, 1069, 1246, 1256, 1295, 1299
NlaIII CATG 7 cut(s) 112, 122, 277, 551, 796, 800, 1014
NlaIV GGNNCC 5 cut(s) 647, 675, 776, 978, 1242
NmuCI GTSAC 2 cut(s) 836, 863
NsiI ATGCAT 1 cut(s) 798
NspI RCATGY 2 cut(s) 112, 551
OliI CACNNNNGTG 2 cut(s) 200, 491
PaeR7I CTCGAG 1 cut(s) 555
PciI ACATGT 1 cut(s) 108
PciSI GCTCTTC 1 cut(s) 436
PfeI GAWTC 3 cut(s) 146, 1056, 1130
PkrI GCNGC 4 cut(s) 553, 666, 910, 1325
PpuMI RGGWCCY 1 cut(s) 645
PscI ACATGT 1 cut(s) 108
Psp5II RGGWCCY 1 cut(s) 645
PspN4I GGNNCC 5 cut(s) 647, 675, 776, 978, 1242
PspPI GGNCC 7 cut(s) 377, 645, 674, 703, 775, 976, 1075
PspPPI RGGWCCY 1 cut(s) 645
PspXI VCTCGAGB 1 cut(s) 555
RsaI GTAC 3 cut(s) 682, 1276, 1331
RsaNI GTAC 3 cut(s) 681, 1275, 1330
RseI CAYNNNNRTG 4 cut(s) 132, 200, 491, 1094
SapI GCTCTTC 1 cut(s) 436
SaqAI TTAA 5 cut(s) 179, 477, 507, 621, 1178
SatI GCNGC 4 cut(s) 552, 665, 909, 1324
Sau3AI GATC 9 cut(s) 124, 277, 923, 927, 1069, 1246, 1256, 1295, 1299
Sau96I GGNCC 7 cut(s) 377, 645, 674, 703, 775, 976, 1075
ScaI AGTACT 1 cut(s) 682
SduI GDGCHC 1 cut(s) 562
SfaNI GCATC 3 cut(s) 93, 417, 1005
Sfr274I CTCGAG 1 cut(s) 555
SinI GGWCC 4 cut(s) 377, 645, 674, 976
SlaI CTCGAG 1 cut(s) 555
SmiMI CAYNNNNRTG 4 cut(s) 132, 200, 491, 1094
SmlI CTYRAG 2 cut(s) 555, 620
SmoI CTYRAG 2 cut(s) 555, 620
Sse9I AATT 9 cut(s) 42, 260, 287, 317, 502, 743, 999, 1180, 1223
SsiI CCGC 2 cut(s) 122, 193
SspI AATATT 1 cut(s) 613
SspMI CTAG 6 cut(s) 381, 438, 723, 1032, 1146, 1372
StyI CCWWGG 2 cut(s) 392, 1078
TaaI ACNGT 3 cut(s) 822, 1211, 1366
TaqI TCGA 6 cut(s) 544, 556, 926, 1255, 1298, 1361
TaqII GACCGA 1 cut(s) 964
TasI AATT 9 cut(s) 42, 260, 287, 317, 502, 743, 999, 1180, 1223
TatI WGTACW 2 cut(s) 680, 1274
TfiI GAWTC 3 cut(s) 146, 1056, 1130
Tru1I TTAA 5 cut(s) 179, 477, 507, 621, 1178
Tru9I TTAA 5 cut(s) 179, 477, 507, 621, 1178
TscAI CASTG 3 cut(s) 769, 889, 1371
TseFI GTSAC 2 cut(s) 836, 863
TseI GCWGC 4 cut(s) 551, 664, 908, 1323
Tsp45I GTSAC 2 cut(s) 836, 863
TspDTI ATGAA 4 cut(s) 108, 262, 294, 781
TspGWI ACGGA 1 cut(s) 212
TspRI CASTG 3 cut(s) 769, 889, 1371
Vha464I CTTAAG 1 cut(s) 620
VpaK11BI GGWCC 4 cut(s) 377, 645, 674, 976
XapI RAATTY 4 cut(s) 287, 502, 743, 1180
XbaI TCTAGA 1 cut(s) 1145
XceI RCATGY 2 cut(s) 112, 551
XcmI CCANNNNNNNNNTGG 1 cut(s) 1213
XhoI CTCGAG 1 cut(s) 555
XspI CTAG 6 cut(s) 381, 438, 723, 1032, 1146, 1372
ZrmI AGTACT 1 cut(s) 682
Zsp2I ATGCAT 1 cut(s) 798
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.